AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0466780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0466780 MtrunA17_Chr3g0120311 99.675 308 1 0 1 308 1 308 0.0 645
Msa0466780 MtrunA17_Chr2g0297021 83.673 245 36 1 15 255 13 257 9.53e-157 439
Msa0466780 MtrunA17_Chr4g0032051 65.269 167 50 3 115 277 23 185 9.34e-59 187
Msa0466780 MtrunA17_Chr4g0032041 80.822 73 14 0 68 140 1 73 1.30e-38 135
Msa0466780 MtrunA17_Chr8g0389111 42.759 145 75 3 18 158 65 205 1.33e-27 110
Msa0466780 MtrunA17_Chr8g0389111 33.129 163 102 4 11 171 249 406 4.93e-18 84.0
Msa0466780 MtrunA17_Chr7g0245591 40.141 142 77 3 16 153 53 190 8.63e-27 108
Msa0466780 MtrunA17_Chr7g0245591 36.893 103 59 3 11 111 251 349 2.63e-13 70.1
Msa0466780 MtrunA17_Chr4g0032031 72.340 47 12 1 15 61 13 58 8.41e-19 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0466780 AT2G32260.1 75.887 282 53 3 12 292 29 296 1.09e-157 444
Msa0466780 AT2G32260.2 74.000 250 50 3 44 292 12 247 3.65e-133 379
Msa0466780 AT2G38670.1 37.037 162 91 4 16 173 53 207 7.72e-26 106
Msa0466780 AT2G38670.1 32.927 164 103 4 11 171 250 409 8.72e-17 80.9

Find 91 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTAGTGTTTCAATTTA+AGG 0.078305 3_3:+67782602 Msa0466780:five_prime_UTR
TTGTGTCTTTCCCATTATTC+TGG 0.120099 3_3:-67785864 None:intergenic
TAAGGCAGTTGGAAGATTTA+AGG 0.165025 3_3:+67784370 Msa0466780:CDS
CTACAACAAGCAAATTTAAT+AGG 0.175743 3_3:-67786351 None:intergenic
AAAACATAGGCGTACATAAA+TGG 0.237318 3_3:+67785889 Msa0466780:exon
GAGCTTAATCCCCAGAATAA+TGG 0.309971 3_3:+67785853 Msa0466780:CDS
TGCAGATATTCTCACCCTTC+AGG 0.311214 3_3:-67786256 None:intergenic
ACACGAACGGGGCGGTCTTC+AGG 0.322066 3_3:-67782801 None:intergenic
TGTGTCTTTCCCATTATTCT+GGG 0.326421 3_3:-67785863 None:intergenic
CCGAATACGTATCTTCTTGT+TGG 0.342507 3_3:+67782986 Msa0466780:CDS
CAGCATTCTTTGCAACAGTT+TGG 0.345244 3_3:-67785468 None:intergenic
CGGAGGGAGGTGAGGGATGG+TGG 0.352026 3_3:-67782778 None:intergenic
CTAATCAGAAACAGAAGTTC+AGG 0.367574 3_3:-67786100 None:intergenic
TAGGAGACTGAAAACTTTGC+AGG 0.370109 3_3:+67784687 Msa0466780:CDS
TACAACAAGCAAATTTAATA+GGG 0.374556 3_3:-67786350 None:intergenic
AGCTTAATCCCCAGAATAAT+GGG 0.375260 3_3:+67785854 Msa0466780:CDS
CATCCACACCAACTTGAGAT+AGG 0.376912 3_3:+67786231 Msa0466780:three_prime_UTR
CGAATTCAAGAAAGTTTAAG+AGG 0.377769 3_3:+67785706 Msa0466780:CDS
TAAATTAGCTCACCATCTTA+TGG 0.378937 3_3:-67785567 None:intergenic
CCAGTATGTGCTGCGTAACT+TGG 0.384421 3_3:+67784457 Msa0466780:CDS
AATGGGAATGAAATATTTGA+TGG 0.404313 3_3:+67786406 Msa0466780:three_prime_UTR
TTCTTGAATTCGATCACATA+TGG 0.409337 3_3:-67785695 None:intergenic
AAAAGCGATTGCGCGTGAAT+AGG 0.423820 3_3:+67784668 Msa0466780:CDS
ATGTTCAGATTACTAGTAAT+GGG 0.426716 3_3:+67786389 Msa0466780:three_prime_UTR
AATGCTGATCGTATGGTTGC+TGG 0.428726 3_3:+67785517 Msa0466780:CDS
CCATCGGCGTAGACACGAAC+GGG 0.432123 3_3:-67782813 None:intergenic
TATGTTCAGATTACTAGTAA+TGG 0.442651 3_3:+67786388 Msa0466780:three_prime_UTR
TTTCTAGAAATGTTTGAAGA+AGG 0.447857 3_3:+67785541 Msa0466780:CDS
GGCGGTCTTCAGGCGGAGGG+AGG 0.449702 3_3:-67782791 None:intergenic
GCAGATATTCTCACCCTTCA+GGG 0.454665 3_3:-67786255 None:intergenic
TCCGATCCCGATTGATTGAT+CGG 0.464162 3_3:-67782739 None:intergenic
CCAACAAGAAGATACGTATT+CGG 0.465493 3_3:-67782986 None:intergenic
CCAAGTTACGCAGCACATAC+TGG 0.466391 3_3:-67784457 None:intergenic
ACGGGGCGGTCTTCAGGCGG+AGG 0.467160 3_3:-67782795 None:intergenic
TTAATAACCCAAGGTGCATC+AGG 0.470523 3_3:-67783219 None:intergenic
TCTTCAGGCGGAGGGAGGTG+AGG 0.478400 3_3:-67782786 None:intergenic
TTTAAGGCAAGGAAAATAAA+CGG 0.487971 3_3:+67782618 Msa0466780:five_prime_UTR
CCCGTTCGTGTCTACGCCGA+TGG 0.493933 3_3:+67782813 Msa0466780:CDS
CTTCAGGCGGAGGGAGGTGA+GGG 0.499646 3_3:-67782785 None:intergenic
TCGATGCAGGTTAAGGCAGT+TGG 0.503231 3_3:+67784359 Msa0466780:intron
AGTAAGAGAACAGCAAGAAA+AGG 0.505554 3_3:+67784714 Msa0466780:CDS
TAGTGTTTCAATTTAAGGCA+AGG 0.522430 3_3:+67782607 Msa0466780:five_prime_UTR
AACTTGAGATAGGGCCCTGA+AGG 0.523113 3_3:+67786241 Msa0466780:three_prime_UTR
CAAACTGTTGCAAAGAATGC+TGG 0.524421 3_3:+67785469 Msa0466780:CDS
GACCACACCAGCAAAATTGA+AGG 0.525013 3_3:-67786023 None:intergenic
AGTGATACCTGATGCACCTT+GGG 0.526304 3_3:+67783212 Msa0466780:CDS
ATCTACGATCTCTTTCACTT+CGG 0.528928 3_3:+67782837 Msa0466780:CDS
TGAAGAAGGTTGCCATAAGA+TGG 0.533046 3_3:+67785555 Msa0466780:CDS
AGGCGGAGGGAGGTGAGGGA+TGG 0.533317 3_3:-67782781 None:intergenic
ACATGTTTCAATGTTGCAGA+AGG 0.535526 3_3:+67785998 Msa0466780:three_prime_UTR
GGCCCTATCTCAAGTTGGTG+TGG 0.535808 3_3:-67786234 None:intergenic
ATCTGCATTTCATTGCTGCA+TGG 0.537462 3_3:+67786272 Msa0466780:three_prime_UTR
TAATCAGAAACAGAAGTTCA+GGG 0.552670 3_3:-67786099 None:intergenic
GCGGTGACACATAAGTATAA+AGG 0.555192 3_3:+67783022 Msa0466780:CDS
GTGTCTTTCCCATTATTCTG+GGG 0.556779 3_3:-67785862 None:intergenic
ATCCACACCAACTTGAGATA+GGG 0.562958 3_3:+67786232 Msa0466780:three_prime_UTR
CCTTGGTGTGAGCTATGTTA+AGG 0.570180 3_3:+67784502 Msa0466780:CDS
CGGGGCGGTCTTCAGGCGGA+GGG 0.571575 3_3:-67782794 None:intergenic
AAGGAAACTCAACGGACTGA+AGG 0.571851 3_3:+67784389 Msa0466780:CDS
ACATAAGTATAAAGGCAAAA+CGG 0.573897 3_3:+67783030 Msa0466780:CDS
ACCGATCAATCAATCGGGAT+CGG 0.575733 3_3:+67782738 Msa0466780:five_prime_UTR
CAGAAACAGAAGTTCAGGGC+AGG 0.577238 3_3:-67786095 None:intergenic
TTCAGGGCCCTATCTCAAGT+TGG 0.580744 3_3:-67786239 None:intergenic
ATGGGAATGAAATATTTGAT+GGG 0.581098 3_3:+67786407 Msa0466780:three_prime_UTR
CATGTTTCAATGTTGCAGAA+GGG 0.585696 3_3:+67785999 Msa0466780:three_prime_UTR
AACTCTTGATTAATAACCCA+AGG 0.585926 3_3:-67783228 None:intergenic
TGCTGCGTAACTTGGATCGT+GGG 0.587350 3_3:+67784465 Msa0466780:CDS
CAAAGAATGCTGGTATGCAT+CGG 0.589636 3_3:+67785479 Msa0466780:CDS
GTGCTGCGTAACTTGGATCG+TGG 0.590786 3_3:+67784464 Msa0466780:CDS
CGAACGGGGCGGTCTTCAGG+CGG 0.603007 3_3:-67782798 None:intergenic
CTACATCAGATATAATAATG+AGG 0.605020 3_3:+67784417 Msa0466780:CDS
GAAAGACACAAACAAAACAT+AGG 0.607776 3_3:+67785876 Msa0466780:CDS
CTGGTATGCATCGGAATGAG+TGG 0.622437 3_3:+67785488 Msa0466780:CDS
TCCATCGGCGTAGACACGAA+CGG 0.624413 3_3:-67782814 None:intergenic
CTTGTTCAAGAGAACGAGCG+TGG 0.627702 3_3:-67782860 None:intergenic
GGTGGAAAATGCTGATCGTA+TGG 0.628102 3_3:+67785510 Msa0466780:CDS
AGAGTATTATGATGATCATG+AGG 0.628279 3_3:+67785786 Msa0466780:CDS
ACTTGAGATAGGGCCCTGAA+GGG 0.630602 3_3:+67786242 Msa0466780:three_prime_UTR
AAGTGATACCTGATGCACCT+TGG 0.631700 3_3:+67783211 Msa0466780:CDS
AAAGAGATCGTAGATTCCAT+CGG 0.647768 3_3:-67782829 None:intergenic
GAAGATTTAAGGAAACTCAA+CGG 0.652437 3_3:+67784381 Msa0466780:CDS
GTATTATGATGATCATGAGG+AGG 0.659585 3_3:+67785789 Msa0466780:CDS
GACAGATATGCTGATACAAG+TGG 0.674347 3_3:+67784225 Msa0466780:intron
TCAAAATGGTAAGGATGACG+AGG 0.681849 3_3:+67785759 Msa0466780:CDS
GGGTACTCAAGAAAAGACCT+TGG 0.686439 3_3:+67784485 Msa0466780:CDS
TGGTATGCATCGGAATGAGT+GGG 0.694195 3_3:+67785489 Msa0466780:CDS
TGTTGGATGCTGCAGCGATG+CGG 0.696674 3_3:+67783003 Msa0466780:CDS
CGGCGTAGACACGAACGGGG+CGG 0.716260 3_3:-67782809 None:intergenic
CATCGGCGTAGACACGAACG+GGG 0.729172 3_3:-67782812 None:intergenic
CCTTAACATAGCTCACACCA+AGG 0.731539 3_3:-67784502 None:intergenic
TATGCATCGGAATGAGTGGG+TGG 0.732959 3_3:+67785492 Msa0466780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATAATCACTTTTTTTTTA+TGG + chr3_3:67782951-67782970 Msa0466780:intron 10.0%
!!! TGATGTATATATATTTTTTT+TGG + chr3_3:67783169-67783188 Msa0466780:intron 10.0%
!!! GATGTATATATATTTTTTTT+GGG + chr3_3:67783170-67783189 Msa0466780:intron 10.0%
!! TAAAAGTTATATTTGAAAAT+TGG + chr3_3:67783571-67783590 Msa0466780:intron 10.0%
!! TGAAATTTAAAAGAAAATTA+AGG - chr3_3:67784307-67784326 None:intergenic 10.0%
!!! AATTTTCTTTTAAATTTCAT+TGG + chr3_3:67784308-67784327 Msa0466780:intron 10.0%
!!! ATTTTCTTTTAAATTTCATT+GGG + chr3_3:67784309-67784328 Msa0466780:intron 10.0%
!! TAAAATAATCGATTAATTGA+GGG - chr3_3:67782932-67782951 None:intergenic 15.0%
!! ATAAAATAATCGATTAATTG+AGG - chr3_3:67782933-67782952 None:intergenic 15.0%
!! ATAAATATACAAAAACGAAA+TGG - chr3_3:67783307-67783326 None:intergenic 15.0%
!! CTTATATTAATGAATTGAAT+TGG - chr3_3:67783494-67783513 None:intergenic 15.0%
!! ATTTGATGTTAGTATAAAAT+TGG + chr3_3:67783885-67783904 Msa0466780:intron 15.0%
!! ATGTAAAATCTATGATTTAA+AGG - chr3_3:67783931-67783950 None:intergenic 15.0%
!!! ATTAATACAATTTTTTCGTT+CGG - chr3_3:67783967-67783986 None:intergenic 15.0%
!!! AAAATATATTGAACATTTTG+AGG - chr3_3:67786202-67786221 None:intergenic 15.0%
!!! CGTTTTTTTGTTAATGTAAT+TGG + chr3_3:67783139-67783158 Msa0466780:intron 20.0%
!! AATGAATTGAATTGGAATAA+AGG - chr3_3:67783486-67783505 None:intergenic 20.0%
!!! AGGTGTATTTTAGTAAATTT+TGG + chr3_3:67783623-67783642 Msa0466780:intron 20.0%
!!! GCTGTATTTTTTAGTTTAAA+GGG + chr3_3:67783735-67783754 Msa0466780:intron 20.0%
!! ATTTAAAGGAAATACATTGT+TGG - chr3_3:67783917-67783936 None:intergenic 20.0%
!! AAATACAAATGTAAGTTTGA+CGG - chr3_3:67784995-67785014 None:intergenic 20.0%
!! AAGTAGATTATCAAAACTTA+AGG + chr3_3:67785314-67785333 Msa0466780:intron 20.0%
!! GAAAAAAGTTTAAAAGCTAT+AGG - chr3_3:67785635-67785654 None:intergenic 20.0%
!!! AGAAAAATTGATTCATCATT+TGG - chr3_3:67785739-67785758 None:intergenic 20.0%
!!! GAATCAATTTTTCTTCAAAA+TGG + chr3_3:67785745-67785764 Msa0466780:CDS 20.0%
!!! AATTTTTCTTCAAAATGGTA+AGG + chr3_3:67785750-67785769 Msa0466780:CDS 20.0%
!! TACAACAAGCAAATTTAATA+GGG - chr3_3:67786353-67786372 None:intergenic 20.0%
!! TTTAAGGCAAGGAAAATAAA+CGG + chr3_3:67782618-67782637 Msa0466780:five_prime_UTR 25.0%
!!! TAATGGAGATTACGATTTTT+TGG - chr3_3:67782886-67782905 None:intergenic 25.0%
! ACATAAGTATAAAGGCAAAA+CGG + chr3_3:67783030-67783049 Msa0466780:CDS 25.0%
! AAAAACGAAATGGAATCTTA+CGG - chr3_3:67783297-67783316 None:intergenic 25.0%
!!! TACTTATAAACTCTAAAGCA+CGG - chr3_3:67783449-67783468 None:intergenic 25.0%
! TTAATATAAGTACCTTTGTC+CGG + chr3_3:67783504-67783523 Msa0466780:intron 25.0%
! GCTTTAATATGAGTTATTCA+TGG + chr3_3:67783527-67783546 Msa0466780:intron 25.0%
!! AATTGTTGTTATACCATACA+TGG - chr3_3:67783696-67783715 None:intergenic 25.0%
! TGGTATAACAACAATTGAAA+TGG + chr3_3:67783700-67783719 Msa0466780:intron 25.0%
! ATTGAAATGGATATCTTCTT+AGG + chr3_3:67783713-67783732 Msa0466780:intron 25.0%
!!! GGCTGTATTTTTTAGTTTAA+AGG + chr3_3:67783734-67783753 Msa0466780:intron 25.0%
!! ACCCATGATTTCTATTTTAT+TGG + chr3_3:67784139-67784158 Msa0466780:intron 25.0%
! GCCAATAAAATAGAAATCAT+GGG - chr3_3:67784143-67784162 None:intergenic 25.0%
! TGCCAATAAAATAGAAATCA+TGG - chr3_3:67784144-67784163 None:intergenic 25.0%
! CTACATCAGATATAATAATG+AGG + chr3_3:67784417-67784436 Msa0466780:CDS 25.0%
! CAAATGGTAGACAATATTAA+GGG - chr3_3:67784603-67784622 None:intergenic 25.0%
! TCAAATGGTAGACAATATTA+AGG - chr3_3:67784604-67784623 None:intergenic 25.0%
! TTAATATTGTCTACCATTTG+AGG + chr3_3:67784603-67784622 Msa0466780:intron 25.0%
!! GTTCATTTTAAATACCAACA+CGG - chr3_3:67784645-67784664 None:intergenic 25.0%
!! TGTTGGTATTTAAAATGAAC+AGG + chr3_3:67784645-67784664 Msa0466780:intron 25.0%
! TACAACTTAGTCAAATGATA+TGG - chr3_3:67784771-67784790 None:intergenic 25.0%
! AGTGAAAAAAGATCATAGAA+TGG + chr3_3:67784840-67784859 Msa0466780:intron 25.0%
! TTCCCTATTTATTACTTTGA+TGG + chr3_3:67784918-67784937 Msa0466780:intron 25.0%
! CTCCATCAAAGTAATAAATA+GGG - chr3_3:67784923-67784942 None:intergenic 25.0%
! TTTCTTGCAATGTTTGTTTA+AGG + chr3_3:67785014-67785033 Msa0466780:intron 25.0%
! TCTATATTCATTGGGTATTT+GGG - chr3_3:67785134-67785153 None:intergenic 25.0%
! TTCTATATTCATTGGGTATT+TGG - chr3_3:67785135-67785154 None:intergenic 25.0%
! AAAATTATGGCTACCTTATA+TGG - chr3_3:67785218-67785237 None:intergenic 25.0%
!!! GCCATAATTTTAACTTTGTT+TGG + chr3_3:67785227-67785246 Msa0466780:intron 25.0%
! GCCAAACAAAGTTAAAATTA+TGG - chr3_3:67785231-67785250 None:intergenic 25.0%
! ATATATGAAGGCAATGTTTA+AGG + chr3_3:67785254-67785273 Msa0466780:intron 25.0%
! TATATGAAGGCAATGTTTAA+GGG + chr3_3:67785255-67785274 Msa0466780:intron 25.0%
!! TTTCTAGAAATGTTTGAAGA+AGG + chr3_3:67785541-67785560 Msa0466780:CDS 25.0%
!! TTTTCTACCTACATTTAACA+TGG - chr3_3:67786304-67786323 None:intergenic 25.0%
!! AGGTAGAAAATTTTGTTCAA+AGG + chr3_3:67786314-67786333 Msa0466780:three_prime_UTR 25.0%
!! GGTAGAAAATTTTGTTCAAA+GGG + chr3_3:67786315-67786334 Msa0466780:three_prime_UTR 25.0%
! CTACAACAAGCAAATTTAAT+AGG - chr3_3:67786354-67786373 None:intergenic 25.0%
! TATGTTCAGATTACTAGTAA+TGG + chr3_3:67786388-67786407 Msa0466780:three_prime_UTR 25.0%
! ATGTTCAGATTACTAGTAAT+GGG + chr3_3:67786389-67786408 Msa0466780:three_prime_UTR 25.0%
! AATGGGAATGAAATATTTGA+TGG + chr3_3:67786406-67786425 Msa0466780:three_prime_UTR 25.0%
! ATGGGAATGAAATATTTGAT+GGG + chr3_3:67786407-67786426 Msa0466780:three_prime_UTR 25.0%
GCAACTAGTGTTTCAATTTA+AGG + chr3_3:67782602-67782621 Msa0466780:five_prime_UTR 30.0%
! TAGTGTTTCAATTTAAGGCA+AGG + chr3_3:67782607-67782626 Msa0466780:five_prime_UTR 30.0%
!! TTGATTGATCGGTTTTTGTT+CGG - chr3_3:67782731-67782750 None:intergenic 30.0%
ACAAAAACCGATCAATCAAT+CGG + chr3_3:67782732-67782751 Msa0466780:five_prime_UTR 30.0%
AAGTAGAGAATGAAGAGTAA+TGG - chr3_3:67782903-67782922 None:intergenic 30.0%
!!! ATTTTTTTTGGGTGTGTAGA+TGG + chr3_3:67783181-67783200 Msa0466780:intron 30.0%
!!! TTTTTTTTGGGTGTGTAGAT+GGG + chr3_3:67783182-67783201 Msa0466780:intron 30.0%
AACTCTTGATTAATAACCCA+AGG - chr3_3:67783231-67783250 None:intergenic 30.0%
! GAATGATGTCATTTGCTTAA+AGG + chr3_3:67783396-67783415 Msa0466780:intron 30.0%
TAATATAAGTACCTTTGTCC+GGG + chr3_3:67783505-67783524 Msa0466780:intron 30.0%
GAATAACTCATATTAAAGCC+CGG - chr3_3:67783526-67783545 None:intergenic 30.0%
!! AGGATAAGTTGAAAAGCATT+CGG - chr3_3:67783672-67783691 None:intergenic 30.0%
TTAAAACTGATGATTTGACG+TGG - chr3_3:67783827-67783846 None:intergenic 30.0%
TAAAATGTAGTTACACGAGT+CGG + chr3_3:67783844-67783863 Msa0466780:intron 30.0%
TCCTGCTTATTTAATGTCTA+TGG + chr3_3:67784001-67784020 Msa0466780:intron 30.0%
TCCATAGACATTAAATAAGC+AGG - chr3_3:67784005-67784024 None:intergenic 30.0%
TACCTAAGAACTAAGAGTTA+GGG - chr3_3:67784105-67784124 None:intergenic 30.0%
ACATTTCATTGCAAAAACCA+AGG - chr3_3:67784204-67784223 None:intergenic 30.0%
GAAGATTTAAGGAAACTCAA+CGG + chr3_3:67784381-67784400 Msa0466780:CDS 30.0%
GCTCCATCAAAGTAATAAAT+AGG - chr3_3:67784924-67784943 None:intergenic 30.0%
! CACAAAATTTTACAGCTCAT+GGG - chr3_3:67785085-67785104 None:intergenic 30.0%
! CATGAGCTGTAAAATTTTGT+GGG + chr3_3:67785084-67785103 Msa0466780:intron 30.0%
! ATGAGCTGTAAAATTTTGTG+GGG + chr3_3:67785085-67785104 Msa0466780:intron 30.0%
ATACCCAATGAATATAGAAG+TGG + chr3_3:67785136-67785155 Msa0466780:intron 30.0%
TCTCCACTTCTATATTCATT+GGG - chr3_3:67785142-67785161 None:intergenic 30.0%
TTGTTTGGCAGTATATATGA+AGG + chr3_3:67785242-67785261 Msa0466780:intron 30.0%
TCACTCGCAACTATAATTTA+TGG + chr3_3:67785370-67785389 Msa0466780:intron 30.0%
!!! GCAGGTAATTTTAAAATGCA+TGG - chr3_3:67785398-67785417 None:intergenic 30.0%
!!! CATTTTAAAATTACCTGCAG+TGG + chr3_3:67785400-67785419 Msa0466780:intron 30.0%
TAAATTAGCTCACCATCTTA+TGG - chr3_3:67785570-67785589 None:intergenic 30.0%
TTCTTGAATTCGATCACATA+TGG - chr3_3:67785698-67785717 None:intergenic 30.0%
CGAATTCAAGAAAGTTTAAG+AGG + chr3_3:67785706-67785725 Msa0466780:CDS 30.0%
! AGAGTATTATGATGATCATG+AGG + chr3_3:67785786-67785805 Msa0466780:CDS 30.0%
GAAAGACACAAACAAAACAT+AGG + chr3_3:67785876-67785895 Msa0466780:CDS 30.0%
AAAACATAGGCGTACATAAA+TGG + chr3_3:67785889-67785908 Msa0466780:exon 30.0%
TAATCAGAAACAGAAGTTCA+GGG - chr3_3:67786102-67786121 None:intergenic 30.0%
! GTTTTTGCCATGTTAAATGT+AGG + chr3_3:67786294-67786313 Msa0466780:three_prime_UTR 30.0%
ATAAAAAAGCGGAAACAACG+CGG + chr3_3:67782663-67782682 Msa0466780:five_prime_UTR 35.0%
CAAAAACCGATCAATCAATC+GGG + chr3_3:67782733-67782752 Msa0466780:five_prime_UTR 35.0%
AAAGAGATCGTAGATTCCAT+CGG - chr3_3:67782832-67782851 None:intergenic 35.0%
ATCTACGATCTCTTTCACTT+CGG + chr3_3:67782837-67782856 Msa0466780:CDS 35.0%
CCAACAAGAAGATACGTATT+CGG - chr3_3:67782989-67783008 None:intergenic 35.0%
TGTAAAACTAAGTTGCACTG+TGG - chr3_3:67783775-67783794 None:intergenic 35.0%
AGAACACTCTCATTAGATGT+CGG - chr3_3:67783798-67783817 None:intergenic 35.0%
AAAATGTAGTTACACGAGTC+GGG + chr3_3:67783845-67783864 Msa0466780:intron 35.0%
GTACCTAAGAACTAAGAGTT+AGG - chr3_3:67784106-67784125 None:intergenic 35.0%
TATGATTTCGATGCAGGTTA+AGG + chr3_3:67784352-67784371 Msa0466780:intron 35.0%
TAAGGCAGTTGGAAGATTTA+AGG + chr3_3:67784370-67784389 Msa0466780:CDS 35.0%
! GCTATGTTAAGGTGTGATTT+TGG + chr3_3:67784513-67784532 Msa0466780:intron 35.0%
ATATTGTCTACCATTTGAGG+AGG + chr3_3:67784606-67784625 Msa0466780:intron 35.0%
AGTAAGAGAACAGCAAGAAA+AGG + chr3_3:67784714-67784733 Msa0466780:CDS 35.0%
!! CAAGAAAAGGTTGGTGTAAA+AGG + chr3_3:67784727-67784746 Msa0466780:intron 35.0%
CTTCCACAAGTTCAACTATT+AGG + chr3_3:67784951-67784970 Msa0466780:intron 35.0%
GAACCTAATAGTTGAACTTG+TGG - chr3_3:67784957-67784976 None:intergenic 35.0%
! CCACAAAATTTTACAGCTCA+TGG - chr3_3:67785086-67785105 None:intergenic 35.0%
! CCATGAGCTGTAAAATTTTG+TGG + chr3_3:67785083-67785102 Msa0466780:intron 35.0%
TTGTGGGGTAAATAAAAGAG+TGG + chr3_3:67785100-67785119 Msa0466780:intron 35.0%
TGTGGGGTAAATAAAAGAGT+GGG + chr3_3:67785101-67785120 Msa0466780:intron 35.0%
GTCTCCACTTCTATATTCAT+TGG - chr3_3:67785143-67785162 None:intergenic 35.0%
ACCTCAAATATTTGTGCAGT+AGG + chr3_3:67785337-67785356 Msa0466780:intron 35.0%
TAAAATTACCTGCAGTGGAT+TGG + chr3_3:67785405-67785424 Msa0466780:intron 35.0%
GTATTATGATGATCATGAGG+AGG + chr3_3:67785789-67785808 Msa0466780:CDS 35.0%
AGCTTAATCCCCAGAATAAT+GGG + chr3_3:67785854-67785873 Msa0466780:CDS 35.0%
TGTGTCTTTCCCATTATTCT+GGG - chr3_3:67785866-67785885 None:intergenic 35.0%
TTGTGTCTTTCCCATTATTC+TGG - chr3_3:67785867-67785886 None:intergenic 35.0%
ACATGTTTCAATGTTGCAGA+AGG + chr3_3:67785998-67786017 Msa0466780:three_prime_UTR 35.0%
CATGTTTCAATGTTGCAGAA+GGG + chr3_3:67785999-67786018 Msa0466780:three_prime_UTR 35.0%
CTAATCAGAAACAGAAGTTC+AGG - chr3_3:67786103-67786122 None:intergenic 35.0%
CCGAATACGTATCTTCTTGT+TGG + chr3_3:67782986-67783005 Msa0466780:CDS 40.0%
GCGGTGACACATAAGTATAA+AGG + chr3_3:67783022-67783041 Msa0466780:CDS 40.0%
TTAATAACCCAAGGTGCATC+AGG - chr3_3:67783222-67783241 None:intergenic 40.0%
TACGTATGTCATTGTCGTGA+TGG - chr3_3:67783368-67783387 None:intergenic 40.0%
TCATATTAAAGCCCGGACAA+AGG - chr3_3:67783519-67783538 None:intergenic 40.0%
TTGACGTAAACGAGTGTGTT+AGG + chr3_3:67783603-67783622 Msa0466780:intron 40.0%
TCAACTTATCCTGCCATGTA+TGG + chr3_3:67783680-67783699 Msa0466780:intron 40.0%
GTTGTTATACCATACATGGC+AGG - chr3_3:67783692-67783711 None:intergenic 40.0%
TAGGGAGTTGCAACAAGAAA+TGG - chr3_3:67784087-67784106 None:intergenic 40.0%
! CTCCCTAACTCTTAGTTCTT+AGG + chr3_3:67784100-67784119 Msa0466780:intron 40.0%
GCATTATGTAGCTAATGCCT+TGG + chr3_3:67784184-67784203 Msa0466780:intron 40.0%
GACAGATATGCTGATACAAG+TGG + chr3_3:67784225-67784244 Msa0466780:intron 40.0%
GTGTTGTATGATTTCGATGC+AGG + chr3_3:67784346-67784365 Msa0466780:intron 40.0%
AACATAAGCTCCTCCTCAAA+TGG - chr3_3:67784619-67784638 None:intergenic 40.0%
! GAGCTTATGTTTTTCCGTGT+TGG + chr3_3:67784628-67784647 Msa0466780:intron 40.0%
TAGGAGACTGAAAACTTTGC+AGG + chr3_3:67784687-67784706 Msa0466780:CDS 40.0%
AGAGAACAGCAAGAAAAGGT+TGG + chr3_3:67784718-67784737 Msa0466780:intron 40.0%
!! TAGAATGGTAGTTCTAGCTG+TGG + chr3_3:67784855-67784874 Msa0466780:intron 40.0%
TCATGGGATGCGAACAAAAA+AGG - chr3_3:67785069-67785088 None:intergenic 40.0%
GCTAAATGTAGATCGTGAGA+AGG + chr3_3:67785172-67785191 Msa0466780:intron 40.0%
TGCATGGGTACTACCATATA+AGG + chr3_3:67785202-67785221 Msa0466780:intron 40.0%
GCCTACTGCACAAATATTTG+AGG - chr3_3:67785341-67785360 None:intergenic 40.0%
TCAAAATTCCAATCCACTGC+AGG - chr3_3:67785416-67785435 None:intergenic 40.0%
CAGCATTCTTTGCAACAGTT+TGG - chr3_3:67785471-67785490 None:intergenic 40.0%
CAAACTGTTGCAAAGAATGC+TGG + chr3_3:67785469-67785488 Msa0466780:CDS 40.0%
CAAAGAATGCTGGTATGCAT+CGG + chr3_3:67785479-67785498 Msa0466780:CDS 40.0%
TGAAGAAGGTTGCCATAAGA+TGG + chr3_3:67785555-67785574 Msa0466780:CDS 40.0%
TCACTGCATGGGTATATTTC+AGG + chr3_3:67785666-67785685 Msa0466780:intron 40.0%
CACTGCATGGGTATATTTCA+GGG + chr3_3:67785667-67785686 Msa0466780:intron 40.0%
ACTGCATGGGTATATTTCAG+GGG + chr3_3:67785668-67785687 Msa0466780:intron 40.0%
TCAAAATGGTAAGGATGACG+AGG + chr3_3:67785759-67785778 Msa0466780:CDS 40.0%
GAGCTTAATCCCCAGAATAA+TGG + chr3_3:67785853-67785872 Msa0466780:CDS 40.0%
GTGTCTTTCCCATTATTCTG+GGG - chr3_3:67785865-67785884 None:intergenic 40.0%
ATCCACACCAACTTGAGATA+GGG + chr3_3:67786232-67786251 Msa0466780:three_prime_UTR 40.0%
ATCTGCATTTCATTGCTGCA+TGG + chr3_3:67786272-67786291 Msa0466780:three_prime_UTR 40.0%
ACCGATCAATCAATCGGGAT+CGG + chr3_3:67782738-67782757 Msa0466780:five_prime_UTR 45.0%
TCCGATCCCGATTGATTGAT+CGG - chr3_3:67782742-67782761 None:intergenic 45.0%
!!! TTTTTGGGTGTGTAGATGGG+TGG + chr3_3:67783185-67783204 Msa0466780:intron 45.0%
AAGTGATACCTGATGCACCT+TGG + chr3_3:67783211-67783230 Msa0466780:CDS 45.0%
AGTGATACCTGATGCACCTT+GGG + chr3_3:67783212-67783231 Msa0466780:CDS 45.0%
!! AAGGAAACTCAACGGACTGA+AGG + chr3_3:67784389-67784408 Msa0466780:CDS 45.0%
GGGTACTCAAGAAAAGACCT+TGG + chr3_3:67784485-67784504 Msa0466780:CDS 45.0%
CCTTAACATAGCTCACACCA+AGG - chr3_3:67784505-67784524 None:intergenic 45.0%
! CCTTGGTGTGAGCTATGTTA+AGG + chr3_3:67784502-67784521 Msa0466780:CDS 45.0%
AAAAGCGATTGCGCGTGAAT+AGG + chr3_3:67784668-67784687 Msa0466780:CDS 45.0%
TAGTTCACTGTGCATGCACT+TGG + chr3_3:67784791-67784810 Msa0466780:intron 45.0%
AGTTCACTGTGCATGCACTT+GGG + chr3_3:67784792-67784811 Msa0466780:intron 45.0%
TGTAGATCGTGAGAAGGAGT+AGG + chr3_3:67785178-67785197 Msa0466780:intron 45.0%
TGAGAAGGAGTAGGATGCAT+GGG + chr3_3:67785187-67785206 Msa0466780:intron 45.0%
TGGTATGCATCGGAATGAGT+GGG + chr3_3:67785489-67785508 Msa0466780:CDS 45.0%
GGTGGAAAATGCTGATCGTA+TGG + chr3_3:67785510-67785529 Msa0466780:CDS 45.0%
AATGCTGATCGTATGGTTGC+TGG + chr3_3:67785517-67785536 Msa0466780:CDS 45.0%
! TCTGTTGCTTGTCACTGCAT+GGG + chr3_3:67785655-67785674 Msa0466780:intron 45.0%
! GAAGGGACCTTCAATTTTGC+TGG + chr3_3:67786016-67786035 Msa0466780:three_prime_UTR 45.0%
! GACCTTCAATTTTGCTGGTG+TGG + chr3_3:67786021-67786040 Msa0466780:three_prime_UTR 45.0%
GACCACACCAGCAAAATTGA+AGG - chr3_3:67786026-67786045 None:intergenic 45.0%
CATCCACACCAACTTGAGAT+AGG + chr3_3:67786231-67786250 Msa0466780:three_prime_UTR 45.0%
GCAGATATTCTCACCCTTCA+GGG - chr3_3:67786258-67786277 None:intergenic 45.0%
TGCAGATATTCTCACCCTTC+AGG - chr3_3:67786259-67786278 None:intergenic 45.0%
!! TTTAAAAATAAATAAAAAAG+CGG + chr3_3:67782652-67782671 Msa0466780:five_prime_UTR 5.0%
CTTGTTCAAGAGAACGAGCG+TGG - chr3_3:67782863-67782882 None:intergenic 50.0%
TCGATGCAGGTTAAGGCAGT+TGG + chr3_3:67784359-67784378 Msa0466780:intron 50.0%
CCAAGTTACGCAGCACATAC+TGG - chr3_3:67784460-67784479 None:intergenic 50.0%
CCAGTATGTGCTGCGTAACT+TGG + chr3_3:67784457-67784476 Msa0466780:CDS 50.0%
TGCTGCGTAACTTGGATCGT+GGG + chr3_3:67784465-67784484 Msa0466780:CDS 50.0%
GTTCACTGTGCATGCACTTG+GGG + chr3_3:67784793-67784812 Msa0466780:intron 50.0%
GTGAGAAGGAGTAGGATGCA+TGG + chr3_3:67785186-67785205 Msa0466780:intron 50.0%
CTGGTATGCATCGGAATGAG+TGG + chr3_3:67785488-67785507 Msa0466780:CDS 50.0%
TATGCATCGGAATGAGTGGG+TGG + chr3_3:67785492-67785511 Msa0466780:CDS 50.0%
! CTCTGTTGCTTGTCACTGCA+TGG + chr3_3:67785654-67785673 Msa0466780:intron 50.0%
CAGAAACAGAAGTTCAGGGC+AGG - chr3_3:67786098-67786117 None:intergenic 50.0%
TTCAGGGCCCTATCTCAAGT+TGG - chr3_3:67786242-67786261 None:intergenic 50.0%
AACTTGAGATAGGGCCCTGA+AGG + chr3_3:67786241-67786260 Msa0466780:three_prime_UTR 50.0%
ACTTGAGATAGGGCCCTGAA+GGG + chr3_3:67786242-67786261 Msa0466780:three_prime_UTR 50.0%
TCCATCGGCGTAGACACGAA+CGG - chr3_3:67782817-67782836 None:intergenic 55.0%
TGTTGGATGCTGCAGCGATG+CGG + chr3_3:67783003-67783022 Msa0466780:CDS 55.0%
GTGCTGCGTAACTTGGATCG+TGG + chr3_3:67784464-67784483 Msa0466780:CDS 55.0%
ACTGTGCATGCACTTGGGGA+CGG + chr3_3:67784797-67784816 Msa0466780:intron 55.0%
!! GGCCCTATCTCAAGTTGGTG+TGG - chr3_3:67786237-67786256 None:intergenic 55.0%
CATCGGCGTAGACACGAACG+GGG - chr3_3:67782815-67782834 None:intergenic 60.0%
CCATCGGCGTAGACACGAAC+GGG - chr3_3:67782816-67782835 None:intergenic 60.0%
CTGTGCATGCACTTGGGGAC+GGG + chr3_3:67784798-67784817 Msa0466780:intron 60.0%
TGTGCATGCACTTGGGGACG+GGG + chr3_3:67784799-67784818 Msa0466780:intron 60.0%
CTTCAGGCGGAGGGAGGTGA+GGG - chr3_3:67782788-67782807 None:intergenic 65.0%
TCTTCAGGCGGAGGGAGGTG+AGG - chr3_3:67782789-67782808 None:intergenic 65.0%
ACACGAACGGGGCGGTCTTC+AGG - chr3_3:67782804-67782823 None:intergenic 65.0%
CCCGTTCGTGTCTACGCCGA+TGG + chr3_3:67782813-67782832 Msa0466780:CDS 65.0%
ATGCACTTGGGGACGGGGCA+AGG + chr3_3:67784804-67784823 Msa0466780:intron 65.0%
TGCACTTGGGGACGGGGCAA+GGG + chr3_3:67784805-67784824 Msa0466780:intron 65.0%
CGGAGGGAGGTGAGGGATGG+TGG - chr3_3:67782781-67782800 None:intergenic 70.0%
AGGCGGAGGGAGGTGAGGGA+TGG - chr3_3:67782784-67782803 None:intergenic 70.0%
CGAACGGGGCGGTCTTCAGG+CGG - chr3_3:67782801-67782820 None:intergenic 70.0%
CGGCGTAGACACGAACGGGG+CGG - chr3_3:67782812-67782831 None:intergenic 70.0%
TGGGGACGGGGCAAGGGAAG+AGG + chr3_3:67784811-67784830 Msa0466780:intron 70.0%
GGCGGTCTTCAGGCGGAGGG+AGG - chr3_3:67782794-67782813 None:intergenic 75.0%
CGGGGCGGTCTTCAGGCGGA+GGG - chr3_3:67782797-67782816 None:intergenic 75.0%
ACGGGGCGGTCTTCAGGCGG+AGG - chr3_3:67782798-67782817 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 67782584 67786471 67782584 ID=Msa0466780;Name=Msa0466780
chr3_3 mRNA 67782584 67786471 67782584 ID=Msa0466780-mRNA-1;Parent=Msa0466780;Name=Msa0466780-mRNA-1;_AED=0.05;_eAED=0.05;_QI=181|1|1|1|1|1|8|574|308
chr3_3 exon 67782584 67782892 67782584 ID=Msa0466780-mRNA-1:exon:15597;Parent=Msa0466780-mRNA-1
chr3_3 exon 67782982 67783095 67782982 ID=Msa0466780-mRNA-1:exon:15598;Parent=Msa0466780-mRNA-1
chr3_3 exon 67783200 67783295 67783200 ID=Msa0466780-mRNA-1:exon:15599;Parent=Msa0466780-mRNA-1
chr3_3 exon 67784230 67784272 67784230 ID=Msa0466780-mRNA-1:exon:15600;Parent=Msa0466780-mRNA-1
chr3_3 exon 67784368 67784523 67784368 ID=Msa0466780-mRNA-1:exon:15601;Parent=Msa0466780-mRNA-1
chr3_3 exon 67784667 67784735 67784667 ID=Msa0466780-mRNA-1:exon:15602;Parent=Msa0466780-mRNA-1
chr3_3 exon 67785466 67785576 67785466 ID=Msa0466780-mRNA-1:exon:15603;Parent=Msa0466780-mRNA-1
chr3_3 exon 67785688 67786471 67785688 ID=Msa0466780-mRNA-1:exon:15604;Parent=Msa0466780-mRNA-1
chr3_3 five_prime_UTR 67782584 67782764 67782584 ID=Msa0466780-mRNA-1:five_prime_utr;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67782765 67782892 67782765 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67782982 67783095 67782982 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67783200 67783295 67783200 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67784230 67784272 67784230 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67784368 67784523 67784368 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67784667 67784735 67784667 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67785466 67785576 67785466 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 CDS 67785688 67785897 67785688 ID=Msa0466780-mRNA-1:cds;Parent=Msa0466780-mRNA-1
chr3_3 three_prime_UTR 67785898 67786471 67785898 ID=Msa0466780-mRNA-1:three_prime_utr;Parent=Msa0466780-mRNA-1
Gene Sequence

>Msa0466780

ATGACGTCATCATCCACCATCCCTCACCTCCCTCCGCCTGAAGACCGCCCCGTTCGTGTCTACGCCGATGGAATCTACGATCTCTTTCACTTCGGCCACGCTCGTTCTCTTGAACAAGCCAAAAAATCATTTCCGAATACGTATCTTCTTGTTGGATGCTGCAGCGATGCGGTGACACATAAGTATAAAGGCAAAACGGTTATGACTGAAGATGAACGTTATGAGTCTCTTCGTCACTGCAAATGGGTGGATGAAGTGATACCTGATGCACCTTGGGTTATTAATCAAGAGTTTCTTGATAAAAATAATATTGACTTTGTTGCTCATGATTCTCTTCCATATGCTGATACAAGTGGTGCTGCAAATGATGTTTATGAATTTGTTAAGGCAGTTGGAAGATTTAAGGAAACTCAACGGACTGAAGGTATATCTACATCAGATATAATAATGAGGATTGTCAAAGACTATAACCAGTATGTGCTGCGTAACTTGGATCGTGGGTACTCAAGAAAAGACCTTGGTGTGAGCTATGTTAAGGAAAAGCGATTGCGCGTGAATAGGAGACTGAAAACTTTGCAGGAGAAAGTAAGAGAACAGCAAGAAAAGATCCAAACTGTTGCAAAGAATGCTGGTATGCATCGGAATGAGTGGGTGGAAAATGCTGATCGTATGGTTGCTGGATTTCTAGAAATGTTTGAAGAAGGTTGCCATAAGATGGGGACTGCCATATGTGATCGAATTCAAGAAAGTTTAAGAGGTCAGCAACCAAATGATGAATCAATTTTTCTTCAAAATGGTAAGGATGACGAGGATGAAGAGTATTATGATGATCATGAGGAGGATAGTGATGAAGAATTTTTTGAAGAATATTTTGATGACAATGAGCTTAATCCCCAGAATAATGGGAAAGACACAAACAAAACATAG
Protein sequence

>Msa0466780

MTSSSTIPHLPPPEDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTYLLVGCCSDAVTHKYKGKTVMTEDERYESLRHCKWVDEVIPDAPWVINQEFLDKNNIDFVAHDSLPYADTSGAANDVYEFVKAVGRFKETQRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKDLGVSYVKEKRLRVNRRLKTLQEKVREQQEKIQTVAKNAGMHRNEWVENADRMVAGFLEMFEEGCHKMGTAICDRIQESLRGQQPNDESIFLQNGKDDEDEEYYDDHEEDSDEEFFEEYFDDNELNPQNNGKDTNKT*