AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1066350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1066350 MtrunA17_Chr7g0246911 97.531 81 2 0 1 81 26 106 5.02e-57 170
Msa1066350 MtrunA17_Chr2g0295971 96.296 81 3 0 1 81 1 81 4.33e-56 167
Msa1066350 MtrunA17_Chr4g0001451 66.667 51 15 2 6 54 18 68 3.14e-15 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1066350 AT5G27700.1 86.420 81 11 0 1 81 1 81 1.77e-51 155
Msa1066350 AT3G53890.2 81.481 81 15 0 1 81 1 81 2.77e-49 150
Msa1066350 AT3G53890.1 81.481 81 15 0 1 81 1 81 2.77e-49 150

Find 35 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTACGAAAGAGACTATAAT+TGG 0.219965 7_2:-60314583 Msa1066350:five_prime_UTR
CTGAAATATCAAAATCTGTT+TGG 0.308490 7_2:+60314541 Msa1066340:intron
AGCTCTACATTCCCAGAAAA+TGG 0.339849 7_2:-60314409 Msa1066350:intron
TCAGCTTAACATCGGTCATT+TGG 0.359324 7_2:-60314257 Msa1066350:CDS
TTTGGGCCGGGTTTCCGATA+AGG 0.361056 7_2:+60314559 Msa1066340:intron
TTTGATATTTCAGGGTTTAT+GGG 0.363565 7_2:-60314531 Msa1066350:five_prime_UTR
AGACTATAATTGGGCCTTAT+CGG 0.369470 7_2:-60314573 Msa1066350:five_prime_UTR
TGAAATATCAAAATCTGTTT+GGG 0.391013 7_2:+60314542 Msa1066340:intron
AGGGCAGTGATAATAAAGTT+AGG 0.392952 7_2:+60312720 Msa1066340:intron
ATAAGAGCAACTTTAGTTGA+AGG 0.410555 7_2:+60312794 Msa1066340:intron
ATGGATATGACCAGCGGGGC+AGG 0.415695 7_2:+60312700 Msa1066340:intron
CTGTGGCTTCACTCGCGCAC+AGG 0.458896 7_2:-60314191 Msa1066350:intron
ATATCAAAATCTGTTTGGGC+CGG 0.487729 7_2:+60314546 Msa1066340:intron
TACTGCCAAGGATCACGCTT+CGG 0.492236 7_2:-60314281 Msa1066350:CDS
CTACGAAAGAGACTATAATT+GGG 0.506090 7_2:-60314582 Msa1066350:five_prime_UTR
TATCAAAATCTGTTTGGGCC+GGG 0.513720 7_2:+60314547 Msa1066340:intron
GAAGCCACAGAGGGCAAAAG+TGG 0.538865 7_2:+60314204 Msa1066340:intron
CCAATTATAGTCTCTTTCGT+AGG 0.541435 7_2:+60314583 Msa1066340:intron
CTGAACCGAAGCGTGATCCT+TGG 0.542452 7_2:+60314276 Msa1066340:intron
GGGCAGTGATAATAAAGTTA+GGG 0.549706 7_2:+60312721 Msa1066340:intron
GAGTAATGGATATGACCAGC+GGG 0.552850 7_2:+60312695 Msa1066340:intron
AAATCGATTGATTACTGCCA+AGG 0.573907 7_2:-60314293 Msa1066350:CDS
TGGATATGACCAGCGGGGCA+GGG 0.578095 7_2:+60312701 Msa1066340:intron
TGATGGATGTAATGGAGTAA+TGG 0.584506 7_2:+60312681 Msa1066340:intron
TGCGCGAGTGAAGCCACAGA+GGG 0.586789 7_2:+60314195 Msa1066340:intron
TCAACACATAATAAAGAAAG+CGG 0.615160 7_2:+60312892 Msa1066340:intron
GATGAAAGCGGTGTCTACAA+TGG 0.621212 7_2:-60314235 Msa1066350:CDS
TTCCAGGGAGATGCTGACAG+TGG 0.628839 7_2:-60312970 Msa1066350:intron
ATTGGGCCTTATCGGAAACC+CGG 0.637805 7_2:-60314565 Msa1066350:five_prime_UTR
ATCGGTCATTTGGATGAAAG+CGG 0.639908 7_2:-60314247 Msa1066350:CDS
GCTTCGGTTCAGCTTAACAT+CGG 0.677252 7_2:-60314265 Msa1066350:CDS
ACAGTGGAATTGATCGCTTG+TGG 0.696079 7_2:-60312954 Msa1066350:CDS
GGAGTAATGGATATGACCAG+CGG 0.702187 7_2:+60312694 Msa1066340:intron
GTGCGCGAGTGAAGCCACAG+AGG 0.703435 7_2:+60314194 Msa1066340:intron
AGTAATGGATATGACCAGCG+GGG 0.780033 7_2:+60312696 Msa1066340:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTTTTAATTTCTTTT+TGG - chr7_2:60314504-60314523 Msa1066350:five_prime_UTR 10.0%
!!! ATCTTTTTTTAATTTCTTTT+TGG - chr7_2:60314504-60314523 Msa1066350:five_prime_UTR 10.0%
!!! ACATAATTTTAATTAGCTTT+AGG - chr7_2:60313291-60313310 Msa1066350:intron 15.0%
!! ATATAAATCAGAATACAATA+TGG + chr7_2:60314231-60314250 Msa1066340:intron 15.0%
!!! ACATAATTTTAATTAGCTTT+AGG - chr7_2:60313291-60313310 Msa1066350:intron 15.0%
!! ATATAAATCAGAATACAATA+TGG + chr7_2:60314231-60314250 Msa1066340:intron 15.0%
!! TGAAATATCAAAATCTGTTT+GGG + chr7_2:60312784-60312803 Msa1066340:intron 20.0%
!!! AAACAGATTTTGATATTTCA+GGG - chr7_2:60312784-60312803 Msa1066350:three_prime_UTR 20.0%
!! ATTCAATACTAATCATGTTT+AGG - chr7_2:60313184-60313203 Msa1066350:intron 20.0%
!! TTCAATACTAATCATGTTTA+GGG - chr7_2:60313185-60313204 Msa1066350:intron 20.0%
!! ATCAGGAAATAAAGTTAAAA+TGG - chr7_2:60313340-60313359 Msa1066350:intron 20.0%
!! ATCTTAATTAAGGAACTATT+TGG + chr7_2:60313658-60313677 Msa1066340:intron 20.0%
!! AAATAGTTCCTTAATTAAGA+TGG - chr7_2:60313657-60313676 Msa1066350:intron 20.0%
!!! CAAAAAAGAGCTTTTTATAA+GGG + chr7_2:60313708-60313727 Msa1066340:intron 20.0%
!!! CTTATAAAAAGCTCTTTTTT+GGG - chr7_2:60313707-60313726 Msa1066350:intron 20.0%
!!! TTATAAAAAGCTCTTTTTTG+GGG - chr7_2:60313708-60313727 Msa1066350:intron 20.0%
!!! TTTATATTTTGTGTAGTATC+TGG - chr7_2:60313741-60313760 Msa1066350:intron 20.0%
!!! TTTTATGAATTGCATTTCTA+AGG - chr7_2:60314302-60314321 Msa1066350:CDS 20.0%
!!! TTATGTCATTTTTATTTCCA+GGG - chr7_2:60314338-60314357 Msa1066350:intron 20.0%
!!! TAAATTTTTGCTAGAGATAT+TGG - chr7_2:60314456-60314475 Msa1066350:exon 20.0%
!!! ATGCAATTAATTTTGTTTGA+AGG + chr7_2:60312695-60312714 Msa1066340:intron 20.0%
!! TGAAATATCAAAATCTGTTT+GGG + chr7_2:60312784-60312803 Msa1066340:intron 20.0%
!!! AAACAGATTTTGATATTTCA+GGG - chr7_2:60312784-60312803 Msa1066350:three_prime_UTR 20.0%
!! ATTCAATACTAATCATGTTT+AGG - chr7_2:60313184-60313203 Msa1066350:intron 20.0%
!! TTCAATACTAATCATGTTTA+GGG - chr7_2:60313185-60313204 Msa1066350:intron 20.0%
!! ATCAGGAAATAAAGTTAAAA+TGG - chr7_2:60313340-60313359 Msa1066350:intron 20.0%
!! ATCTTAATTAAGGAACTATT+TGG + chr7_2:60313658-60313677 Msa1066340:intron 20.0%
!! AAATAGTTCCTTAATTAAGA+TGG - chr7_2:60313657-60313676 Msa1066350:intron 20.0%
!!! CAAAAAAGAGCTTTTTATAA+GGG + chr7_2:60313708-60313727 Msa1066340:intron 20.0%
!!! CTTATAAAAAGCTCTTTTTT+GGG - chr7_2:60313707-60313726 Msa1066350:intron 20.0%
!!! TTATAAAAAGCTCTTTTTTG+GGG - chr7_2:60313708-60313727 Msa1066350:intron 20.0%
!!! TTTATATTTTGTGTAGTATC+TGG - chr7_2:60313741-60313760 Msa1066350:intron 20.0%
!!! TTTTATGAATTGCATTTCTA+AGG - chr7_2:60314302-60314321 Msa1066350:CDS 20.0%
!!! TTATGTCATTTTTATTTCCA+GGG - chr7_2:60314338-60314357 Msa1066350:intron 20.0%
!!! TAAATTTTTGCTAGAGATAT+TGG - chr7_2:60314456-60314475 Msa1066350:exon 20.0%
! CTGAAATATCAAAATCTGTT+TGG + chr7_2:60312785-60312804 Msa1066340:intron 25.0%
!!! CAAACAGATTTTGATATTTC+AGG - chr7_2:60312783-60312802 Msa1066350:three_prime_UTR 25.0%
!! TTTTGATATTTCAGGGTTTA+TGG - chr7_2:60312791-60312810 Msa1066350:three_prime_UTR 25.0%
! TTTGATATTTCAGGGTTTAT+GGG - chr7_2:60312792-60312811 Msa1066350:three_prime_UTR 25.0%
!! CAAAGTATATACCATTTTCT+GGG + chr7_2:60312928-60312947 Msa1066340:intron 25.0%
!! TCAAAGTATATACCATTTTC+TGG + chr7_2:60312929-60312948 Msa1066340:intron 25.0%
!!! CGTTATTTGTTGATTTGATT+TGG - chr7_2:60312958-60312977 Msa1066350:CDS 25.0%
!!! TTGTTGATTTGATTTGGTTA+TGG - chr7_2:60312964-60312983 Msa1066350:CDS 25.0%
!! CATGTTTAGGGTATTTAAAT+TGG - chr7_2:60313197-60313216 Msa1066350:intron 25.0%
! AGTAAACAACATAAATGATC+AGG - chr7_2:60313323-60313342 Msa1066350:intron 25.0%
! CATATAGCTAATATAGCTAT+AGG + chr7_2:60313370-60313389 Msa1066340:intron 25.0%
! TTAACAAATTATTATCGACG+TGG - chr7_2:60313537-60313556 Msa1066350:intron 25.0%
! AGAATCAACCATCTTAATTA+AGG + chr7_2:60313668-60313687 Msa1066340:intron 25.0%
!! CCAAAAAAGAGCTTTTTATA+AGG + chr7_2:60313709-60313728 Msa1066340:intron 25.0%
!!! CCTTATAAAAAGCTCTTTTT+TGG - chr7_2:60313706-60313725 Msa1066350:intron 25.0%
! CAACTAATACTTATAATCTG+CGG - chr7_2:60313945-60313964 Msa1066350:intron 25.0%
! AGTGAAAAAGTAAAAGTGAA+TGG - chr7_2:60313988-60314007 Msa1066350:intron 25.0%
! TGAAAATTGATATGTTGCAA+TGG - chr7_2:60314049-60314068 Msa1066350:intron 25.0%
! TGTTGAAAGTTAATGAAGTT+GGG - chr7_2:60314148-60314167 Msa1066350:intron 25.0%
! TTTAACCTTTATAAACACGT+TGG - chr7_2:60314191-60314210 Msa1066350:intron 25.0%
! TAATTCCAACGTGTTTATAA+AGG + chr7_2:60314199-60314218 Msa1066340:intron 25.0%
!!! GTTATGTCATTTTTATTTCC+AGG - chr7_2:60314337-60314356 Msa1066350:intron 25.0%
! TCAACACATAATAAAGAAAG+CGG + chr7_2:60314434-60314453 Msa1066340:intron 25.0%
! CTGAAATATCAAAATCTGTT+TGG + chr7_2:60312785-60312804 Msa1066340:intron 25.0%
!!! CAAACAGATTTTGATATTTC+AGG - chr7_2:60312783-60312802 Msa1066350:three_prime_UTR 25.0%
!! TTTTGATATTTCAGGGTTTA+TGG - chr7_2:60312791-60312810 Msa1066350:three_prime_UTR 25.0%
! TTTGATATTTCAGGGTTTAT+GGG - chr7_2:60312792-60312811 Msa1066350:three_prime_UTR 25.0%
!! CAAAGTATATACCATTTTCT+GGG + chr7_2:60312928-60312947 Msa1066340:intron 25.0%
!! TCAAAGTATATACCATTTTC+TGG + chr7_2:60312929-60312948 Msa1066340:intron 25.0%
!!! CGTTATTTGTTGATTTGATT+TGG - chr7_2:60312958-60312977 Msa1066350:CDS 25.0%
!!! TTGTTGATTTGATTTGGTTA+TGG - chr7_2:60312964-60312983 Msa1066350:CDS 25.0%
!! CATGTTTAGGGTATTTAAAT+TGG - chr7_2:60313197-60313216 Msa1066350:intron 25.0%
! AGTAAACAACATAAATGATC+AGG - chr7_2:60313323-60313342 Msa1066350:intron 25.0%
! CATATAGCTAATATAGCTAT+AGG + chr7_2:60313370-60313389 Msa1066340:intron 25.0%
! TTAACAAATTATTATCGACG+TGG - chr7_2:60313537-60313556 Msa1066350:intron 25.0%
! AGAATCAACCATCTTAATTA+AGG + chr7_2:60313668-60313687 Msa1066340:intron 25.0%
!! CCAAAAAAGAGCTTTTTATA+AGG + chr7_2:60313709-60313728 Msa1066340:intron 25.0%
!!! CCTTATAAAAAGCTCTTTTT+TGG - chr7_2:60313706-60313725 Msa1066350:intron 25.0%
! CAACTAATACTTATAATCTG+CGG - chr7_2:60313945-60313964 Msa1066350:intron 25.0%
! AGTGAAAAAGTAAAAGTGAA+TGG - chr7_2:60313988-60314007 Msa1066350:intron 25.0%
! TGAAAATTGATATGTTGCAA+TGG - chr7_2:60314049-60314068 Msa1066350:intron 25.0%
! TGTTGAAAGTTAATGAAGTT+GGG - chr7_2:60314148-60314167 Msa1066350:intron 25.0%
! TTTAACCTTTATAAACACGT+TGG - chr7_2:60314191-60314210 Msa1066350:intron 25.0%
! TAATTCCAACGTGTTTATAA+AGG + chr7_2:60314199-60314218 Msa1066340:intron 25.0%
!!! GTTATGTCATTTTTATTTCC+AGG - chr7_2:60314337-60314356 Msa1066350:intron 25.0%
! TCAACACATAATAAAGAAAG+CGG + chr7_2:60314434-60314453 Msa1066340:intron 25.0%
CTACGAAAGAGACTATAATT+GGG - chr7_2:60312741-60312760 Msa1066350:three_prime_UTR 30.0%
! TTCTTCGTTCTGCATTTTTA+GGG + chr7_2:60312881-60312900 Msa1066340:intron 30.0%
TAAAAATGCAGAACGAAGAA+GGG - chr7_2:60312881-60312900 Msa1066350:three_prime_UTR 30.0%
! ATTGGATTTTGCATTTGTCT+TGG - chr7_2:60313215-60313234 Msa1066350:intron 30.0%
! TTGGATTTTGCATTTGTCTT+GGG - chr7_2:60313216-60313235 Msa1066350:intron 30.0%
GTGTTGAAAGTTAATGAAGT+TGG - chr7_2:60314147-60314166 Msa1066350:intron 30.0%
GAACAACATAGTATGTTCAA+TGG + chr7_2:60314257-60314276 Msa1066340:intron 30.0%
TCATAAAACCTGAATCATCA+AGG + chr7_2:60314290-60314309 Msa1066340:intron 30.0%
ATAAGAGCAACTTTAGTTGA+AGG + chr7_2:60314532-60314551 Msa1066340:intron 30.0%
CTACGAAAGAGACTATAATT+GGG - chr7_2:60312741-60312760 Msa1066350:three_prime_UTR 30.0%
! TTCTTCGTTCTGCATTTTTA+GGG + chr7_2:60312881-60312900 Msa1066340:intron 30.0%
TAAAAATGCAGAACGAAGAA+GGG - chr7_2:60312881-60312900 Msa1066350:three_prime_UTR 30.0%
! ATTGGATTTTGCATTTGTCT+TGG - chr7_2:60313215-60313234 Msa1066350:intron 30.0%
! TTGGATTTTGCATTTGTCTT+GGG - chr7_2:60313216-60313235 Msa1066350:intron 30.0%
GTGTTGAAAGTTAATGAAGT+TGG - chr7_2:60314147-60314166 Msa1066350:intron 30.0%
GAACAACATAGTATGTTCAA+TGG + chr7_2:60314257-60314276 Msa1066340:intron 30.0%
TCATAAAACCTGAATCATCA+AGG + chr7_2:60314290-60314309 Msa1066340:intron 30.0%
ATAAGAGCAACTTTAGTTGA+AGG + chr7_2:60314532-60314551 Msa1066340:intron 30.0%
CCAATTATAGTCTCTTTCGT+AGG + chr7_2:60312743-60312762 Msa1066340:intron 35.0%
CCTACGAAAGAGACTATAAT+TGG - chr7_2:60312740-60312759 Msa1066350:three_prime_UTR 35.0%
! AGACTATAATTGGGCCTTAT+CGG - chr7_2:60312750-60312769 Msa1066350:three_prime_UTR 35.0%
ATATCAAAATCTGTTTGGGC+CGG + chr7_2:60312780-60312799 Msa1066340:intron 35.0%
! CTTCTTCGTTCTGCATTTTT+AGG + chr7_2:60312882-60312901 Msa1066340:intron 35.0%
CTAAAAATGCAGAACGAAGA+AGG - chr7_2:60312880-60312899 Msa1066350:three_prime_UTR 35.0%
AAATCGATTGATTACTGCCA+AGG - chr7_2:60313030-60313049 Msa1066350:intron 35.0%
TCATAGGTTGTGAGACTATT+TGG + chr7_2:60313424-60313443 Msa1066340:intron 35.0%
ACAACCATTTGCAAAGTTGT+GGG + chr7_2:60313611-60313630 Msa1066340:intron 35.0%
!!! GCTCTTTTTTGGGGAAAATT+TGG - chr7_2:60313717-60313736 Msa1066350:intron 35.0%
TTGTCCTGTATTTCATTCTG+TGG - chr7_2:60313775-60313794 Msa1066350:intron 35.0%
TGATTCCAAGTGAACAAAGA+AGG - chr7_2:60313854-60313873 Msa1066350:intron 35.0%
ATTGTCCTTCTTTGTTCACT+TGG + chr7_2:60313862-60313881 Msa1066340:intron 35.0%
AAGTGAACAAAGAAGGACAA+TGG - chr7_2:60313861-60313880 Msa1066350:intron 35.0%
ACTTTCTCATACTCTTCTTG+CGG + chr7_2:60313971-60313990 Msa1066340:intron 35.0%
GTTAATGAAGTTGGGAACTA+TGG - chr7_2:60314156-60314175 Msa1066350:intron 35.0%
! TTGATACACCTTGATGATTC+AGG - chr7_2:60314279-60314298 Msa1066350:CDS 35.0%
GGGCAGTGATAATAAAGTTA+GGG + chr7_2:60314605-60314624 Msa1066340:intron 35.0%
AGGGCAGTGATAATAAAGTT+AGG + chr7_2:60314606-60314625 Msa1066340:intron 35.0%
CCAATTATAGTCTCTTTCGT+AGG + chr7_2:60312743-60312762 Msa1066340:intron 35.0%
CCTACGAAAGAGACTATAAT+TGG - chr7_2:60312740-60312759 Msa1066350:three_prime_UTR 35.0%
! AGACTATAATTGGGCCTTAT+CGG - chr7_2:60312750-60312769 Msa1066350:three_prime_UTR 35.0%
ATATCAAAATCTGTTTGGGC+CGG + chr7_2:60312780-60312799 Msa1066340:intron 35.0%
! CTTCTTCGTTCTGCATTTTT+AGG + chr7_2:60312882-60312901 Msa1066340:intron 35.0%
CTAAAAATGCAGAACGAAGA+AGG - chr7_2:60312880-60312899 Msa1066350:three_prime_UTR 35.0%
AAATCGATTGATTACTGCCA+AGG - chr7_2:60313030-60313049 Msa1066350:intron 35.0%
TCATAGGTTGTGAGACTATT+TGG + chr7_2:60313424-60313443 Msa1066340:intron 35.0%
ACAACCATTTGCAAAGTTGT+GGG + chr7_2:60313611-60313630 Msa1066340:intron 35.0%
!!! GCTCTTTTTTGGGGAAAATT+TGG - chr7_2:60313717-60313736 Msa1066350:intron 35.0%
TTGTCCTGTATTTCATTCTG+TGG - chr7_2:60313775-60313794 Msa1066350:intron 35.0%
TGATTCCAAGTGAACAAAGA+AGG - chr7_2:60313854-60313873 Msa1066350:intron 35.0%
ATTGTCCTTCTTTGTTCACT+TGG + chr7_2:60313862-60313881 Msa1066340:intron 35.0%
AAGTGAACAAAGAAGGACAA+TGG - chr7_2:60313861-60313880 Msa1066350:intron 35.0%
ACTTTCTCATACTCTTCTTG+CGG + chr7_2:60313971-60313990 Msa1066340:intron 35.0%
GTTAATGAAGTTGGGAACTA+TGG - chr7_2:60314156-60314175 Msa1066350:intron 35.0%
! TTGATACACCTTGATGATTC+AGG - chr7_2:60314279-60314298 Msa1066350:CDS 35.0%
GGGCAGTGATAATAAAGTTA+GGG + chr7_2:60314605-60314624 Msa1066340:intron 35.0%
AGGGCAGTGATAATAAAGTT+AGG + chr7_2:60314606-60314625 Msa1066340:intron 35.0%
TATCAAAATCTGTTTGGGCC+GGG + chr7_2:60312779-60312798 Msa1066340:intron 40.0%
! CGATTTTGTGTGTTTTGCGA+AGG - chr7_2:60312837-60312856 Msa1066350:three_prime_UTR 40.0%
!! TTTTCTGGGAATGTAGAGCT+CGG + chr7_2:60312914-60312933 Msa1066340:intron 40.0%
AGCTCTACATTCCCAGAAAA+TGG - chr7_2:60312914-60312933 Msa1066350:exon 40.0%
TCAGCTTAACATCGGTCATT+TGG - chr7_2:60313066-60313085 Msa1066350:intron 40.0%
ATCGGTCATTTGGATGAAAG+CGG - chr7_2:60313076-60313095 Msa1066350:intron 40.0%
TATTATCGACGTGGACTTGT+CGG - chr7_2:60313546-60313565 Msa1066350:intron 40.0%
ATAGCCCACAACTTTGCAAA+TGG - chr7_2:60313604-60313623 Msa1066350:intron 40.0%
CACAACCATTTGCAAAGTTG+TGG + chr7_2:60313612-60313631 Msa1066340:intron 40.0%
CAACTTTGCAAATGGTTGTG+TGG - chr7_2:60313612-60313631 Msa1066350:intron 40.0%
AGCTCCACAGAATGAAATAC+AGG + chr7_2:60313782-60313801 Msa1066340:intron 40.0%
TATCAAAATCTGTTTGGGCC+GGG + chr7_2:60312779-60312798 Msa1066340:intron 40.0%
! CGATTTTGTGTGTTTTGCGA+AGG - chr7_2:60312837-60312856 Msa1066350:three_prime_UTR 40.0%
!! TTTTCTGGGAATGTAGAGCT+CGG + chr7_2:60312914-60312933 Msa1066340:intron 40.0%
AGCTCTACATTCCCAGAAAA+TGG - chr7_2:60312914-60312933 Msa1066350:exon 40.0%
TCAGCTTAACATCGGTCATT+TGG - chr7_2:60313066-60313085 Msa1066350:intron 40.0%
ATCGGTCATTTGGATGAAAG+CGG - chr7_2:60313076-60313095 Msa1066350:intron 40.0%
TATTATCGACGTGGACTTGT+CGG - chr7_2:60313546-60313565 Msa1066350:intron 40.0%
ATAGCCCACAACTTTGCAAA+TGG - chr7_2:60313604-60313623 Msa1066350:intron 40.0%
CACAACCATTTGCAAAGTTG+TGG + chr7_2:60313612-60313631 Msa1066340:intron 40.0%
CAACTTTGCAAATGGTTGTG+TGG - chr7_2:60313612-60313631 Msa1066350:intron 40.0%
AGCTCCACAGAATGAAATAC+AGG + chr7_2:60313782-60313801 Msa1066340:intron 40.0%
GCTTCGGTTCAGCTTAACAT+CGG - chr7_2:60313058-60313077 Msa1066350:intron 45.0%
GATGAAAGCGGTGTCTACAA+TGG - chr7_2:60313088-60313107 Msa1066350:intron 45.0%
!! TTTTCCACTTTTGCCCTCTG+TGG - chr7_2:60313115-60313134 Msa1066350:intron 45.0%
GTCTCACAACCTATGAAGCA+CGG - chr7_2:60313428-60313447 Msa1066350:intron 45.0%
ATGAAGCACGGAAACAAACC+CGG + chr7_2:60313586-60313605 Msa1066340:intron 45.0%
! AAGTTGTGGGCTATGAAGCA+CGG + chr7_2:60313598-60313617 Msa1066340:intron 45.0%
ACAGTGGAATTGATCGCTTG+TGG - chr7_2:60314369-60314388 Msa1066350:intron 45.0%
!! AGTAATGGATATGACCAGCG+GGG + chr7_2:60314630-60314649 Msa1066340:intron 45.0%
!! GAGTAATGGATATGACCAGC+GGG + chr7_2:60314631-60314650 Msa1066340:intron 45.0%
!! GGAGTAATGGATATGACCAG+CGG + chr7_2:60314632-60314651 Msa1066340:intron 45.0%
GCTTCGGTTCAGCTTAACAT+CGG - chr7_2:60313058-60313077 Msa1066350:intron 45.0%
GATGAAAGCGGTGTCTACAA+TGG - chr7_2:60313088-60313107 Msa1066350:intron 45.0%
!! TTTTCCACTTTTGCCCTCTG+TGG - chr7_2:60313115-60313134 Msa1066350:intron 45.0%
GTCTCACAACCTATGAAGCA+CGG - chr7_2:60313428-60313447 Msa1066350:intron 45.0%
ATGAAGCACGGAAACAAACC+CGG + chr7_2:60313586-60313605 Msa1066340:intron 45.0%
! AAGTTGTGGGCTATGAAGCA+CGG + chr7_2:60313598-60313617 Msa1066340:intron 45.0%
ACAGTGGAATTGATCGCTTG+TGG - chr7_2:60314369-60314388 Msa1066350:intron 45.0%
!! AGTAATGGATATGACCAGCG+GGG + chr7_2:60314630-60314649 Msa1066340:intron 45.0%
!! GAGTAATGGATATGACCAGC+GGG + chr7_2:60314631-60314650 Msa1066340:intron 45.0%
!! GGAGTAATGGATATGACCAG+CGG + chr7_2:60314632-60314651 Msa1066340:intron 45.0%
! ATTGGGCCTTATCGGAAACC+CGG - chr7_2:60312758-60312777 Msa1066350:three_prime_UTR 50.0%
TACTGCCAAGGATCACGCTT+CGG - chr7_2:60313042-60313061 Msa1066350:intron 50.0%
TATGAAGCACGGACACTCCT+CGG - chr7_2:60313439-60313458 Msa1066350:intron 50.0%
! GTCGGTGTCTATGTCATGTC+CGG - chr7_2:60313564-60313583 Msa1066350:intron 50.0%
! TCGGTGTCTATGTCATGTCC+GGG - chr7_2:60313565-60313584 Msa1066350:intron 50.0%
! ATTGGGCCTTATCGGAAACC+CGG - chr7_2:60312758-60312777 Msa1066350:three_prime_UTR 50.0%
TACTGCCAAGGATCACGCTT+CGG - chr7_2:60313042-60313061 Msa1066350:intron 50.0%
TATGAAGCACGGACACTCCT+CGG - chr7_2:60313439-60313458 Msa1066350:intron 50.0%
! GTCGGTGTCTATGTCATGTC+CGG - chr7_2:60313564-60313583 Msa1066350:intron 50.0%
! TCGGTGTCTATGTCATGTCC+GGG - chr7_2:60313565-60313584 Msa1066350:intron 50.0%
TTTGGGCCGGGTTTCCGATA+AGG + chr7_2:60312767-60312786 Msa1066340:intron 55.0%
CTGAACCGAAGCGTGATCCT+TGG + chr7_2:60313050-60313069 Msa1066340:intron 55.0%
GAAGCCACAGAGGGCAAAAG+TGG + chr7_2:60313122-60313141 Msa1066340:intron 55.0%
!! GAGGAGTGTCCGTGCTTCAT+AGG + chr7_2:60313440-60313459 Msa1066340:intron 55.0%
! TCCTCGGATTAGGTCTGTCC+CGG - chr7_2:60313455-60313474 Msa1066350:intron 55.0%
GATTAGGTCTGTCCCGGTGT+CGG - chr7_2:60313461-60313480 Msa1066350:intron 55.0%
TTCCAGGGAGATGCTGACAG+TGG - chr7_2:60314353-60314372 Msa1066350:intron 55.0%
TTCCACTGTCAGCATCTCCC+TGG + chr7_2:60314358-60314377 Msa1066340:intron 55.0%
TTTGGGCCGGGTTTCCGATA+AGG + chr7_2:60312767-60312786 Msa1066340:intron 55.0%
CTGAACCGAAGCGTGATCCT+TGG + chr7_2:60313050-60313069 Msa1066340:intron 55.0%
GAAGCCACAGAGGGCAAAAG+TGG + chr7_2:60313122-60313141 Msa1066340:intron 55.0%
!! GAGGAGTGTCCGTGCTTCAT+AGG + chr7_2:60313440-60313459 Msa1066340:intron 55.0%
! TCCTCGGATTAGGTCTGTCC+CGG - chr7_2:60313455-60313474 Msa1066350:intron 55.0%
GATTAGGTCTGTCCCGGTGT+CGG - chr7_2:60313461-60313480 Msa1066350:intron 55.0%
TTCCAGGGAGATGCTGACAG+TGG - chr7_2:60314353-60314372 Msa1066350:intron 55.0%
TTCCACTGTCAGCATCTCCC+TGG + chr7_2:60314358-60314377 Msa1066340:intron 55.0%
TGCGCGAGTGAAGCCACAGA+GGG + chr7_2:60313131-60313150 Msa1066340:intron 60.0%
GCACGGACACTCCTCGGATT+AGG - chr7_2:60313445-60313464 Msa1066350:intron 60.0%
ACCGGGACAGACCTAATCCG+AGG + chr7_2:60313459-60313478 Msa1066340:intron 60.0%
TGGATATGACCAGCGGGGCA+GGG + chr7_2:60314625-60314644 Msa1066340:intron 60.0%
ATGGATATGACCAGCGGGGC+AGG + chr7_2:60314626-60314645 Msa1066340:intron 60.0%
TGCGCGAGTGAAGCCACAGA+GGG + chr7_2:60313131-60313150 Msa1066340:intron 60.0%
GCACGGACACTCCTCGGATT+AGG - chr7_2:60313445-60313464 Msa1066350:intron 60.0%
ACCGGGACAGACCTAATCCG+AGG + chr7_2:60313459-60313478 Msa1066340:intron 60.0%
TGGATATGACCAGCGGGGCA+GGG + chr7_2:60314625-60314644 Msa1066340:intron 60.0%
ATGGATATGACCAGCGGGGC+AGG + chr7_2:60314626-60314645 Msa1066340:intron 60.0%
GTGCGCGAGTGAAGCCACAG+AGG + chr7_2:60313132-60313151 Msa1066340:intron 65.0%
CTGTGGCTTCACTCGCGCAC+AGG - chr7_2:60313132-60313151 Msa1066350:intron 65.0%
ACCAACGCGTGTCCGACACC+GGG + chr7_2:60313476-60313495 Msa1066340:intron 65.0%
CACCAACGCGTGTCCGACAC+CGG + chr7_2:60313477-60313496 Msa1066340:intron 65.0%
TTATCACTGCCCTGCCCCGC+TGG - chr7_2:60314613-60314632 Msa1066350:five_prime_UTR 65.0%
GTGCGCGAGTGAAGCCACAG+AGG + chr7_2:60313132-60313151 Msa1066340:intron 65.0%
CTGTGGCTTCACTCGCGCAC+AGG - chr7_2:60313132-60313151 Msa1066350:intron 65.0%
ACCAACGCGTGTCCGACACC+GGG + chr7_2:60313476-60313495 Msa1066340:intron 65.0%
CACCAACGCGTGTCCGACAC+CGG + chr7_2:60313477-60313496 Msa1066340:intron 65.0%
TTATCACTGCCCTGCCCCGC+TGG - chr7_2:60314613-60314632 Msa1066350:five_prime_UTR 65.0%
! TCCCGGTGTCGGACACGCGT+TGG - chr7_2:60313472-60313491 Msa1066350:intron 70.0%
! TCCCGGTGTCGGACACGCGT+TGG - chr7_2:60313472-60313491 Msa1066350:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 60312694 60314651 60312694 ID=Msa1066350;Name=Msa1066350
chr7_2 mRNA 60312694 60314651 60312694 ID=Msa1066350-mRNA-1;Parent=Msa1066350;Name=Msa1066350-mRNA-1;_AED=0.08;_eAED=0.08;_QI=192|1|1|1|1|1|3|230|81
chr7_2 exon 60312694 60312986 60312694 ID=Msa1066350-mRNA-1:exon:11853;Parent=Msa1066350-mRNA-1
chr7_2 exon 60314192 60314324 60314192 ID=Msa1066350-mRNA-1:exon:11852;Parent=Msa1066350-mRNA-1
chr7_2 exon 60314410 60314651 60314410 ID=Msa1066350-mRNA-1:exon:11851;Parent=Msa1066350-mRNA-1
chr7_2 five_prime_UTR 60314460 60314651 60314460 ID=Msa1066350-mRNA-1:five_prime_utr;Parent=Msa1066350-mRNA-1
chr7_2 CDS 60314410 60314459 60314410 ID=Msa1066350-mRNA-1:cds;Parent=Msa1066350-mRNA-1
chr7_2 CDS 60314192 60314324 60314192 ID=Msa1066350-mRNA-1:cds;Parent=Msa1066350-mRNA-1
chr7_2 CDS 60312924 60312986 60312924 ID=Msa1066350-mRNA-1:cds;Parent=Msa1066350-mRNA-1
chr7_2 three_prime_UTR 60312694 60312923 60312694 ID=Msa1066350-mRNA-1:three_prime_utr;Parent=Msa1066350-mRNA-1
Gene Sequence

>Msa1066350

ATGCAGAACGAAGAAGGGCAAATCACCGAGCTCTACATTCCCAGAAAATGTTCTGCTACAAATCGATTGATTACTGCCAAGGATCACGCTTCGGTTCAGCTTAACATCGGTCATTTGGATGAAAGCGGTGTCTACAATGGTCAATTTTCCACTTTTGCCCTCTGTGGCTTCACTCGCGCACAGGGAGATGCTGACAGTGGAATTGATCGCTTGTGGCAGAAAAAGAAAGCTGAAATTAAGCAATAG
Protein sequence

>Msa1066350

MQNEEGQITELYIPRKCSATNRLITAKDHASVQLNIGHLDESGVYNGQFSTFALCGFTRAQGDADSGIDRLWQKKKAEIKQ*