AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1115510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1115510 MtrunA17_Chr7g0246911 97.727 88 2 0 18 105 19 106 7.93e-62 183
Msa1115510 MtrunA17_Chr2g0295971 96.296 81 3 0 25 105 1 81 6.67e-56 167
Msa1115510 MtrunA17_Chr4g0001451 66.667 51 15 2 30 78 18 68 8.98e-15 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1115510 AT5G27700.1 86.420 81 11 0 25 105 1 81 3.00e-51 156
Msa1115510 AT3G53890.2 81.481 81 15 0 25 105 1 81 5.62e-49 150
Msa1115510 AT3G53890.1 81.481 81 15 0 25 105 1 81 5.62e-49 150

Find 36 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATGAAAGAGACTATAAT+TGG 0.221286 7_3:-62961032 Msa1115510:five_prime_UTR
CTGAAATATCAAAATCTGTT+TGG 0.308490 7_3:+62960990 Msa1115500:intron
AGCTCTACATTCCCAGAAAA+TGG 0.339849 7_3:-62960862 Msa1115510:intron
GGGCAGTGATAATAAAAGTT+AGG 0.348961 7_3:+62959168 Msa1115500:intron
TCAGCTTAACATCGGTCATT+TGG 0.359324 7_3:-62960720 Msa1115510:CDS
TTTGGGCCGGGTTTCCGATA+AGG 0.361056 7_3:+62961008 Msa1115500:intron
TTTGATATTTCAGGGTTTAT+GGG 0.363565 7_3:-62960980 Msa1115510:exon
AGACTATAATTGGGCCTTAT+CGG 0.369470 7_3:-62961022 Msa1115510:five_prime_UTR
GAATGTAGAGCTCGGTGATT+TGG 0.379996 7_3:+62960873 Msa1115500:intron
TGAAATATCAAAATCTGTTT+GGG 0.391013 7_3:+62960991 Msa1115500:intron
CAATTATAGTCTCTTTCATA+AGG 0.392799 7_3:+62961033 Msa1115500:intron
ATAAGAGCAACTTTAGTTGA+AGG 0.410555 7_3:+62959242 Msa1115500:intron
ATGGATATGACCAGCGGGGC+AGG 0.415695 7_3:+62959147 Msa1115500:intron
CTGTGGCTTCACTCGCGCAC+AGG 0.458896 7_3:-62960654 Msa1115510:intron
GGCAGTGATAATAAAAGTTA+GGG 0.471434 7_3:+62959169 Msa1115500:intron
ATATCAAAATCTGTTTGGGC+CGG 0.487729 7_3:+62960995 Msa1115500:intron
TACTGCCAAGGATCACGCTT+CGG 0.492236 7_3:-62960744 Msa1115510:CDS
TATCAAAATCTGTTTGGGCC+GGG 0.513720 7_3:+62960996 Msa1115500:intron
TTATGAAAGAGACTATAATT+GGG 0.530904 7_3:-62961031 Msa1115510:five_prime_UTR
GAAGCCACAGAGGGCAAAAG+TGG 0.538865 7_3:+62960667 Msa1115500:intron
CTGAACCGAAGCGTGATCCT+TGG 0.542452 7_3:+62960739 Msa1115500:intron
GAGTAATGGATATGACCAGC+GGG 0.552850 7_3:+62959142 Msa1115500:intron
TGATGGATGTAATGGAGTAA+TGG 0.562609 7_3:+62959128 Msa1115500:intron
TGGATATGACCAGCGGGGCA+GGG 0.578095 7_3:+62959148 Msa1115500:intron
TGCGCGAGTGAAGCCACAGA+GGG 0.586789 7_3:+62960658 Msa1115500:intron
AAACAGATTGATTACTGCCA+AGG 0.589977 7_3:-62960756 Msa1115510:CDS
TCAACACATAATAAAGAAAG+CGG 0.615160 7_3:+62959340 Msa1115500:intron
GATGAAAGCGGTGTCTACAA+TGG 0.621212 7_3:-62960698 Msa1115510:CDS
TTCCAGGGAGATGCTGACAG+TGG 0.628839 7_3:-62959418 Msa1115510:intron
ATTGGGCCTTATCGGAAACC+CGG 0.637805 7_3:-62961014 Msa1115510:five_prime_UTR
ATCGGTCATTTGGATGAAAG+CGG 0.639908 7_3:-62960710 Msa1115510:CDS
GCTTCGGTTCAGCTTAACAT+CGG 0.677252 7_3:-62960728 Msa1115510:CDS
ACAGTGGAATTGATCGCTTG+TGG 0.696079 7_3:-62959402 Msa1115510:CDS
GGAGTAATGGATATGACCAG+CGG 0.702187 7_3:+62959141 Msa1115500:intron
GTGCGCGAGTGAAGCCACAG+AGG 0.703435 7_3:+62960657 Msa1115500:intron
AGTAATGGATATGACCAGCG+GGG 0.780033 7_3:+62959143 Msa1115500:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTTTTAATTTCTTTT+TGG - chr7_3:62960918-62960937 Msa1115510:CDS 10.0%
!!! ATCTTTTTTTAATTTCTTTT+TGG - chr7_3:62960918-62960937 Msa1115510:CDS 10.0%
!!! ACATAATTTTAATTAGCTTT+AGG - chr7_3:62959705-62959724 Msa1115510:intron 15.0%
!! ATCATGAAATAAAGTTAAAA+TGG - chr7_3:62959754-62959773 Msa1115510:intron 15.0%
!!! TTTATATTTTGTTTAGTATC+TGG - chr7_3:62960155-62960174 Msa1115510:intron 15.0%
!! ATATAAATCAGAATACAATA+TGG + chr7_3:62960645-62960664 Msa1115500:intron 15.0%
!!! ACATAATTTTAATTAGCTTT+AGG - chr7_3:62959705-62959724 Msa1115510:intron 15.0%
!! ATCATGAAATAAAGTTAAAA+TGG - chr7_3:62959754-62959773 Msa1115510:intron 15.0%
!!! TTTATATTTTGTTTAGTATC+TGG - chr7_3:62960155-62960174 Msa1115510:intron 15.0%
!! ATATAAATCAGAATACAATA+TGG + chr7_3:62960645-62960664 Msa1115500:intron 15.0%
!! TTATGAAAGAGACTATAATT+GGG - chr7_3:62959154-62959173 Msa1115510:three_prime_UTR 20.0%
!! TGAAATATCAAAATCTGTTT+GGG + chr7_3:62959197-62959216 Msa1115500:intron 20.0%
!!! AAACAGATTTTGATATTTCA+GGG - chr7_3:62959197-62959216 Msa1115510:three_prime_UTR 20.0%
!! ATTCAATACTAATCATGTTT+AGG - chr7_3:62959598-62959617 Msa1115510:intron 20.0%
!! TTCAATACTAATCATGTTTA+GGG - chr7_3:62959599-62959618 Msa1115510:intron 20.0%
!! ATCTTAATTAAGGAACTATT+TGG + chr7_3:62960068-62960087 Msa1115500:intron 20.0%
!! AAATAGTTCCTTAATTAAGA+TGG - chr7_3:62960067-62960086 Msa1115510:intron 20.0%
!!! CAAAAAAGAGCTTTTTATAA+GGG + chr7_3:62960122-62960141 Msa1115500:intron 20.0%
!!! CTTATAAAAAGCTCTTTTTT+GGG - chr7_3:62960121-62960140 Msa1115510:intron 20.0%
!!! TTATAAAAAGCTCTTTTTTG+GGG - chr7_3:62960122-62960141 Msa1115510:intron 20.0%
!!! CATATTGAAATATTGCTTTT+AGG - chr7_3:62960503-62960522 Msa1115510:intron 20.0%
!!! TTTTATGAATTGCATTTCTA+AGG - chr7_3:62960716-62960735 Msa1115510:CDS 20.0%
!!! TTATGTCATTTTTATTTCCA+GGG - chr7_3:62960752-62960771 Msa1115510:CDS 20.0%
!!! TAAATTTTTGCTAGAGATAT+TGG - chr7_3:62960870-62960889 Msa1115510:CDS 20.0%
!!! TTTCATAAGGATTTTTTTCT+TGG + chr7_3:62959142-62959161 Msa1115500:intron 20.0%
!! TTATGAAAGAGACTATAATT+GGG - chr7_3:62959154-62959173 Msa1115510:three_prime_UTR 20.0%
!! TGAAATATCAAAATCTGTTT+GGG + chr7_3:62959197-62959216 Msa1115500:intron 20.0%
!!! AAACAGATTTTGATATTTCA+GGG - chr7_3:62959197-62959216 Msa1115510:three_prime_UTR 20.0%
!! ATTCAATACTAATCATGTTT+AGG - chr7_3:62959598-62959617 Msa1115510:intron 20.0%
!! TTCAATACTAATCATGTTTA+GGG - chr7_3:62959599-62959618 Msa1115510:intron 20.0%
!! ATCTTAATTAAGGAACTATT+TGG + chr7_3:62960068-62960087 Msa1115500:intron 20.0%
!! AAATAGTTCCTTAATTAAGA+TGG - chr7_3:62960067-62960086 Msa1115510:intron 20.0%
!!! CAAAAAAGAGCTTTTTATAA+GGG + chr7_3:62960122-62960141 Msa1115500:intron 20.0%
!!! CTTATAAAAAGCTCTTTTTT+GGG - chr7_3:62960121-62960140 Msa1115510:intron 20.0%
!!! TTATAAAAAGCTCTTTTTTG+GGG - chr7_3:62960122-62960141 Msa1115510:intron 20.0%
!!! CATATTGAAATATTGCTTTT+AGG - chr7_3:62960503-62960522 Msa1115510:intron 20.0%
!!! TTTTATGAATTGCATTTCTA+AGG - chr7_3:62960716-62960735 Msa1115510:CDS 20.0%
!!! TTATGTCATTTTTATTTCCA+GGG - chr7_3:62960752-62960771 Msa1115510:CDS 20.0%
!!! TAAATTTTTGCTAGAGATAT+TGG - chr7_3:62960870-62960889 Msa1115510:CDS 20.0%
! CAATTATAGTCTCTTTCATA+AGG + chr7_3:62959155-62959174 Msa1115500:intron 25.0%
! CTTATGAAAGAGACTATAAT+TGG - chr7_3:62959153-62959172 Msa1115510:three_prime_UTR 25.0%
! CTGAAATATCAAAATCTGTT+TGG + chr7_3:62959198-62959217 Msa1115500:intron 25.0%
!!! CAAACAGATTTTGATATTTC+AGG - chr7_3:62959196-62959215 Msa1115510:three_prime_UTR 25.0%
!! TTTTGATATTTCAGGGTTTA+TGG - chr7_3:62959204-62959223 Msa1115510:three_prime_UTR 25.0%
! TTTGATATTTCAGGGTTTAT+GGG - chr7_3:62959205-62959224 Msa1115510:three_prime_UTR 25.0%
!! CAAAGTTTATACCATTTTCT+GGG + chr7_3:62959337-62959356 Msa1115500:intron 25.0%
!! TCAAAGTTTATACCATTTTC+TGG + chr7_3:62959338-62959357 Msa1115500:intron 25.0%
!!! TCTCGTTATTTTCATTGTTA+TGG - chr7_3:62959364-62959383 Msa1115510:exon 25.0%
! TTTAGTGTAATCATATGTGT+CGG + chr7_3:62959913-62959932 Msa1115500:intron 25.0%
! TTAACAAATTATTATCGACG+TGG - chr7_3:62959943-62959962 Msa1115510:intron 25.0%
! AGAATCAACCATCTTAATTA+AGG + chr7_3:62960078-62960097 Msa1115500:intron 25.0%
!! CCAAAAAAGAGCTTTTTATA+AGG + chr7_3:62960123-62960142 Msa1115500:intron 25.0%
!!! CCTTATAAAAAGCTCTTTTT+TGG - chr7_3:62960120-62960139 Msa1115510:intron 25.0%
! CAACTAATACTTATAATCTG+CGG - chr7_3:62960359-62960378 Msa1115510:intron 25.0%
! AGTGAAAAAGTAAAAGTGAA+TGG - chr7_3:62960402-62960421 Msa1115510:intron 25.0%
! TGAAAATTGATATGTTGCAA+TGG - chr7_3:62960463-62960482 Msa1115510:intron 25.0%
! TGTTGAAAGTTAATGAAGTT+GGG - chr7_3:62960562-62960581 Msa1115510:intron 25.0%
! TTTAACCTTTATAAACACGT+TGG - chr7_3:62960605-62960624 Msa1115510:intron 25.0%
! TAATTCCAACGTGTTTATAA+AGG + chr7_3:62960613-62960632 Msa1115500:intron 25.0%
!!! GTTATGTCATTTTTATTTCC+AGG - chr7_3:62960751-62960770 Msa1115510:CDS 25.0%
! TCAACACATAATAAAGAAAG+CGG + chr7_3:62960848-62960867 Msa1115500:intron 25.0%
! CAATTATAGTCTCTTTCATA+AGG + chr7_3:62959155-62959174 Msa1115500:intron 25.0%
! CTTATGAAAGAGACTATAAT+TGG - chr7_3:62959153-62959172 Msa1115510:three_prime_UTR 25.0%
! CTGAAATATCAAAATCTGTT+TGG + chr7_3:62959198-62959217 Msa1115500:intron 25.0%
!!! CAAACAGATTTTGATATTTC+AGG - chr7_3:62959196-62959215 Msa1115510:three_prime_UTR 25.0%
!! TTTTGATATTTCAGGGTTTA+TGG - chr7_3:62959204-62959223 Msa1115510:three_prime_UTR 25.0%
! TTTGATATTTCAGGGTTTAT+GGG - chr7_3:62959205-62959224 Msa1115510:three_prime_UTR 25.0%
!! CAAAGTTTATACCATTTTCT+GGG + chr7_3:62959337-62959356 Msa1115500:intron 25.0%
!! TCAAAGTTTATACCATTTTC+TGG + chr7_3:62959338-62959357 Msa1115500:intron 25.0%
!!! TCTCGTTATTTTCATTGTTA+TGG - chr7_3:62959364-62959383 Msa1115510:exon 25.0%
! TTTAGTGTAATCATATGTGT+CGG + chr7_3:62959913-62959932 Msa1115500:intron 25.0%
! TTAACAAATTATTATCGACG+TGG - chr7_3:62959943-62959962 Msa1115510:intron 25.0%
! AGAATCAACCATCTTAATTA+AGG + chr7_3:62960078-62960097 Msa1115500:intron 25.0%
!! CCAAAAAAGAGCTTTTTATA+AGG + chr7_3:62960123-62960142 Msa1115500:intron 25.0%
!!! CCTTATAAAAAGCTCTTTTT+TGG - chr7_3:62960120-62960139 Msa1115510:intron 25.0%
! CAACTAATACTTATAATCTG+CGG - chr7_3:62960359-62960378 Msa1115510:intron 25.0%
! AGTGAAAAAGTAAAAGTGAA+TGG - chr7_3:62960402-62960421 Msa1115510:intron 25.0%
! TGAAAATTGATATGTTGCAA+TGG - chr7_3:62960463-62960482 Msa1115510:intron 25.0%
! TGTTGAAAGTTAATGAAGTT+GGG - chr7_3:62960562-62960581 Msa1115510:intron 25.0%
! TTTAACCTTTATAAACACGT+TGG - chr7_3:62960605-62960624 Msa1115510:intron 25.0%
! TAATTCCAACGTGTTTATAA+AGG + chr7_3:62960613-62960632 Msa1115500:intron 25.0%
!!! GTTATGTCATTTTTATTTCC+AGG - chr7_3:62960751-62960770 Msa1115510:CDS 25.0%
! TCAACACATAATAAAGAAAG+CGG + chr7_3:62960848-62960867 Msa1115500:intron 25.0%
! TTCTTCGTTCTGCATTTTTA+GGG + chr7_3:62959290-62959309 Msa1115500:intron 30.0%
!! CATGTTTAGGGTATTGAAAT+TGG - chr7_3:62959611-62959630 Msa1115510:intron 30.0%
ATTGGATTGTGTATTTGTCT+TGG - chr7_3:62959629-62959648 Msa1115510:intron 30.0%
TTGGATTGTGTATTTGTCTT+GGG - chr7_3:62959630-62959649 Msa1115510:intron 30.0%
! GTTGATTCTGTTTCTCATAA+TGG - chr7_3:62960089-62960108 Msa1115510:intron 30.0%
GTGTTGAAAGTTAATGAAGT+TGG - chr7_3:62960561-62960580 Msa1115510:intron 30.0%
GAACAACATAGTATGTTCAA+TGG + chr7_3:62960671-62960690 Msa1115500:intron 30.0%
TCATAAAACCTGAATCATCA+AGG + chr7_3:62960704-62960723 Msa1115500:intron 30.0%
ATAAGAGCAACTTTAGTTGA+AGG + chr7_3:62960946-62960965 Msa1115500:intron 30.0%
GGCAGTGATAATAAAAGTTA+GGG + chr7_3:62961019-62961038 Msa1115500:intron 30.0%
! TTCTTCGTTCTGCATTTTTA+GGG + chr7_3:62959290-62959309 Msa1115500:intron 30.0%
!! CATGTTTAGGGTATTGAAAT+TGG - chr7_3:62959611-62959630 Msa1115510:intron 30.0%
ATTGGATTGTGTATTTGTCT+TGG - chr7_3:62959629-62959648 Msa1115510:intron 30.0%
TTGGATTGTGTATTTGTCTT+GGG - chr7_3:62959630-62959649 Msa1115510:intron 30.0%
! GTTGATTCTGTTTCTCATAA+TGG - chr7_3:62960089-62960108 Msa1115510:intron 30.0%
GTGTTGAAAGTTAATGAAGT+TGG - chr7_3:62960561-62960580 Msa1115510:intron 30.0%
GAACAACATAGTATGTTCAA+TGG + chr7_3:62960671-62960690 Msa1115500:intron 30.0%
TCATAAAACCTGAATCATCA+AGG + chr7_3:62960704-62960723 Msa1115500:intron 30.0%
ATAAGAGCAACTTTAGTTGA+AGG + chr7_3:62960946-62960965 Msa1115500:intron 30.0%
GGCAGTGATAATAAAAGTTA+GGG + chr7_3:62961019-62961038 Msa1115500:intron 30.0%
! AGACTATAATTGGGCCTTAT+CGG - chr7_3:62959163-62959182 Msa1115510:three_prime_UTR 35.0%
ATATCAAAATCTGTTTGGGC+CGG + chr7_3:62959193-62959212 Msa1115500:intron 35.0%
! CTTCTTCGTTCTGCATTTTT+AGG + chr7_3:62959291-62959310 Msa1115500:intron 35.0%
CTAAAAATGCAGAACGAAGA+AGG - chr7_3:62959289-62959308 Msa1115510:three_prime_UTR 35.0%
AAACAGATTGATTACTGCCA+AGG - chr7_3:62959429-62959448 Msa1115510:intron 35.0%
GCACAGGTCTCTAATTAATT+TGG - chr7_3:62959547-62959566 Msa1115510:intron 35.0%
TCATAGGTTGTGAGACTATT+TGG + chr7_3:62959830-62959849 Msa1115500:intron 35.0%
!!! GCTCTTTTTTGGGGAAAATT+TGG - chr7_3:62960131-62960150 Msa1115510:intron 35.0%
TTGTCCTGTATTTCATTCTG+TGG - chr7_3:62960189-62960208 Msa1115510:intron 35.0%
TGTCCTGTATTTCATTCTGT+GGG - chr7_3:62960190-62960209 Msa1115510:intron 35.0%
TGATTCCAAGTGAACAAAGA+AGG - chr7_3:62960268-62960287 Msa1115510:intron 35.0%
ATTGTCCTTCTTTGTTCACT+TGG + chr7_3:62960276-62960295 Msa1115500:intron 35.0%
AAGTGAACAAAGAAGGACAA+TGG - chr7_3:62960275-62960294 Msa1115510:intron 35.0%
ACTTTCTCATACTCTTCTTG+CGG + chr7_3:62960385-62960404 Msa1115500:intron 35.0%
GTTAATGAAGTTGGGAACTA+TGG - chr7_3:62960570-62960589 Msa1115510:intron 35.0%
! TTGATACACCTTGATGATTC+AGG - chr7_3:62960693-62960712 Msa1115510:CDS 35.0%
GGGCAGTGATAATAAAAGTT+AGG + chr7_3:62961020-62961039 Msa1115500:intron 35.0%
! AGACTATAATTGGGCCTTAT+CGG - chr7_3:62959163-62959182 Msa1115510:three_prime_UTR 35.0%
ATATCAAAATCTGTTTGGGC+CGG + chr7_3:62959193-62959212 Msa1115500:intron 35.0%
! CTTCTTCGTTCTGCATTTTT+AGG + chr7_3:62959291-62959310 Msa1115500:intron 35.0%
CTAAAAATGCAGAACGAAGA+AGG - chr7_3:62959289-62959308 Msa1115510:three_prime_UTR 35.0%
AAACAGATTGATTACTGCCA+AGG - chr7_3:62959429-62959448 Msa1115510:intron 35.0%
GCACAGGTCTCTAATTAATT+TGG - chr7_3:62959547-62959566 Msa1115510:intron 35.0%
TCATAGGTTGTGAGACTATT+TGG + chr7_3:62959830-62959849 Msa1115500:intron 35.0%
!!! GCTCTTTTTTGGGGAAAATT+TGG - chr7_3:62960131-62960150 Msa1115510:intron 35.0%
TTGTCCTGTATTTCATTCTG+TGG - chr7_3:62960189-62960208 Msa1115510:intron 35.0%
TGTCCTGTATTTCATTCTGT+GGG - chr7_3:62960190-62960209 Msa1115510:intron 35.0%
TGATTCCAAGTGAACAAAGA+AGG - chr7_3:62960268-62960287 Msa1115510:intron 35.0%
ATTGTCCTTCTTTGTTCACT+TGG + chr7_3:62960276-62960295 Msa1115500:intron 35.0%
AAGTGAACAAAGAAGGACAA+TGG - chr7_3:62960275-62960294 Msa1115510:intron 35.0%
ACTTTCTCATACTCTTCTTG+CGG + chr7_3:62960385-62960404 Msa1115500:intron 35.0%
GTTAATGAAGTTGGGAACTA+TGG - chr7_3:62960570-62960589 Msa1115510:intron 35.0%
! TTGATACACCTTGATGATTC+AGG - chr7_3:62960693-62960712 Msa1115510:CDS 35.0%
GGGCAGTGATAATAAAAGTT+AGG + chr7_3:62961020-62961039 Msa1115500:intron 35.0%
TATCAAAATCTGTTTGGGCC+GGG + chr7_3:62959192-62959211 Msa1115500:intron 40.0%
! TACGATTGTGTGTTTTGCGA+AGG - chr7_3:62959246-62959265 Msa1115510:three_prime_UTR 40.0%
!! TTTTCTGGGAATGTAGAGCT+CGG + chr7_3:62959323-62959342 Msa1115500:intron 40.0%
AGCTCTACATTCCCAGAAAA+TGG - chr7_3:62959323-62959342 Msa1115510:three_prime_UTR 40.0%
TCAGCTTAACATCGGTCATT+TGG - chr7_3:62959465-62959484 Msa1115510:intron 40.0%
ATCGGTCATTTGGATGAAAG+CGG - chr7_3:62959475-62959494 Msa1115510:intron 40.0%
TATTATCGACGTGGACTTGT+CGG - chr7_3:62959952-62959971 Msa1115510:intron 40.0%
ACAACCACTTGCAAAGTTGT+GGG + chr7_3:62960017-62960036 Msa1115500:intron 40.0%
TTTGCAAGTGGTTGTGTTTG+TGG - chr7_3:62960022-62960041 Msa1115510:intron 40.0%
GTCCTGTATTTCATTCTGTG+GGG - chr7_3:62960191-62960210 Msa1115510:intron 40.0%
TATCAAAATCTGTTTGGGCC+GGG + chr7_3:62959192-62959211 Msa1115500:intron 40.0%
! TACGATTGTGTGTTTTGCGA+AGG - chr7_3:62959246-62959265 Msa1115510:three_prime_UTR 40.0%
!! TTTTCTGGGAATGTAGAGCT+CGG + chr7_3:62959323-62959342 Msa1115500:intron 40.0%
AGCTCTACATTCCCAGAAAA+TGG - chr7_3:62959323-62959342 Msa1115510:three_prime_UTR 40.0%
TCAGCTTAACATCGGTCATT+TGG - chr7_3:62959465-62959484 Msa1115510:intron 40.0%
ATCGGTCATTTGGATGAAAG+CGG - chr7_3:62959475-62959494 Msa1115510:intron 40.0%
TATTATCGACGTGGACTTGT+CGG - chr7_3:62959952-62959971 Msa1115510:intron 40.0%
ACAACCACTTGCAAAGTTGT+GGG + chr7_3:62960017-62960036 Msa1115500:intron 40.0%
TTTGCAAGTGGTTGTGTTTG+TGG - chr7_3:62960022-62960041 Msa1115510:intron 40.0%
GTCCTGTATTTCATTCTGTG+GGG - chr7_3:62960191-62960210 Msa1115510:intron 40.0%
! GAATGTAGAGCTCGGTGATT+TGG + chr7_3:62959315-62959334 Msa1115500:intron 45.0%
GCTTCGGTTCAGCTTAACAT+CGG - chr7_3:62959457-62959476 Msa1115510:intron 45.0%
GATGAAAGCGGTGTCTACAA+TGG - chr7_3:62959487-62959506 Msa1115510:intron 45.0%
!! TTTTCCACTTTTGCCCTCTG+TGG - chr7_3:62959514-62959533 Msa1115510:intron 45.0%
GTCTCACAACCTATGATGCA+CGG - chr7_3:62959834-62959853 Msa1115510:intron 45.0%
ATGAAGCACGGAAACAAACC+CGG + chr7_3:62959992-62960011 Msa1115500:intron 45.0%
! AAGTTGTGGGCTATGAAGCA+CGG + chr7_3:62960004-62960023 Msa1115500:intron 45.0%
ATAGCCCACAACTTTGCAAG+TGG - chr7_3:62960010-62960029 Msa1115510:intron 45.0%
CACAACCACTTGCAAAGTTG+TGG + chr7_3:62960018-62960037 Msa1115500:intron 45.0%
AGCCCCACAGAATGAAATAC+AGG + chr7_3:62960196-62960215 Msa1115500:intron 45.0%
ACAGTGGAATTGATCGCTTG+TGG - chr7_3:62960783-62960802 Msa1115510:intron 45.0%
!! AGTAATGGATATGACCAGCG+GGG + chr7_3:62961045-62961064 Msa1115500:intron 45.0%
!! GAGTAATGGATATGACCAGC+GGG + chr7_3:62961046-62961065 Msa1115500:intron 45.0%
!! GGAGTAATGGATATGACCAG+CGG + chr7_3:62961047-62961066 Msa1115500:intron 45.0%
! GAATGTAGAGCTCGGTGATT+TGG + chr7_3:62959315-62959334 Msa1115500:intron 45.0%
GCTTCGGTTCAGCTTAACAT+CGG - chr7_3:62959457-62959476 Msa1115510:intron 45.0%
GATGAAAGCGGTGTCTACAA+TGG - chr7_3:62959487-62959506 Msa1115510:intron 45.0%
!! TTTTCCACTTTTGCCCTCTG+TGG - chr7_3:62959514-62959533 Msa1115510:intron 45.0%
GTCTCACAACCTATGATGCA+CGG - chr7_3:62959834-62959853 Msa1115510:intron 45.0%
ATGAAGCACGGAAACAAACC+CGG + chr7_3:62959992-62960011 Msa1115500:intron 45.0%
! AAGTTGTGGGCTATGAAGCA+CGG + chr7_3:62960004-62960023 Msa1115500:intron 45.0%
ATAGCCCACAACTTTGCAAG+TGG - chr7_3:62960010-62960029 Msa1115510:intron 45.0%
CACAACCACTTGCAAAGTTG+TGG + chr7_3:62960018-62960037 Msa1115500:intron 45.0%
AGCCCCACAGAATGAAATAC+AGG + chr7_3:62960196-62960215 Msa1115500:intron 45.0%
ACAGTGGAATTGATCGCTTG+TGG - chr7_3:62960783-62960802 Msa1115510:intron 45.0%
!! AGTAATGGATATGACCAGCG+GGG + chr7_3:62961045-62961064 Msa1115500:intron 45.0%
!! GAGTAATGGATATGACCAGC+GGG + chr7_3:62961046-62961065 Msa1115500:intron 45.0%
!! GGAGTAATGGATATGACCAG+CGG + chr7_3:62961047-62961066 Msa1115500:intron 45.0%
! ATTGGGCCTTATCGGAAACC+CGG - chr7_3:62959171-62959190 Msa1115510:three_prime_UTR 50.0%
TACTGCCAAGGATCACGCTT+CGG - chr7_3:62959441-62959460 Msa1115510:intron 50.0%
TATGATGCACGGACACTCCT+CGG - chr7_3:62959845-62959864 Msa1115510:intron 50.0%
! GTCGGTGTCTATGTCATGTC+CGG - chr7_3:62959970-62959989 Msa1115510:intron 50.0%
! TCGGTGTCTATGTCATGTCC+GGG - chr7_3:62959971-62959990 Msa1115510:intron 50.0%
! ATTGGGCCTTATCGGAAACC+CGG - chr7_3:62959171-62959190 Msa1115510:three_prime_UTR 50.0%
TACTGCCAAGGATCACGCTT+CGG - chr7_3:62959441-62959460 Msa1115510:intron 50.0%
TATGATGCACGGACACTCCT+CGG - chr7_3:62959845-62959864 Msa1115510:intron 50.0%
! GTCGGTGTCTATGTCATGTC+CGG - chr7_3:62959970-62959989 Msa1115510:intron 50.0%
! TCGGTGTCTATGTCATGTCC+GGG - chr7_3:62959971-62959990 Msa1115510:intron 50.0%
TTTGGGCCGGGTTTCCGATA+AGG + chr7_3:62959180-62959199 Msa1115500:intron 55.0%
CTGAACCGAAGCGTGATCCT+TGG + chr7_3:62959449-62959468 Msa1115500:intron 55.0%
GAAGCCACAGAGGGCAAAAG+TGG + chr7_3:62959521-62959540 Msa1115500:intron 55.0%
!! GAGGAGTGTCCGTGCATCAT+AGG + chr7_3:62959846-62959865 Msa1115500:intron 55.0%
! TCCTCGGATTAGGTCTGTCC+CGG - chr7_3:62959861-62959880 Msa1115510:intron 55.0%
GATTAGGTCTGTCCCGGTGT+CGG - chr7_3:62959867-62959886 Msa1115510:intron 55.0%
TTCCAGGGAGATGCTGACAG+TGG - chr7_3:62960767-62960786 Msa1115510:CDS 55.0%
TTCCACTGTCAGCATCTCCC+TGG + chr7_3:62960772-62960791 Msa1115500:intron 55.0%
TTTGGGCCGGGTTTCCGATA+AGG + chr7_3:62959180-62959199 Msa1115500:intron 55.0%
CTGAACCGAAGCGTGATCCT+TGG + chr7_3:62959449-62959468 Msa1115500:intron 55.0%
GAAGCCACAGAGGGCAAAAG+TGG + chr7_3:62959521-62959540 Msa1115500:intron 55.0%
!! GAGGAGTGTCCGTGCATCAT+AGG + chr7_3:62959846-62959865 Msa1115500:intron 55.0%
! TCCTCGGATTAGGTCTGTCC+CGG - chr7_3:62959861-62959880 Msa1115510:intron 55.0%
GATTAGGTCTGTCCCGGTGT+CGG - chr7_3:62959867-62959886 Msa1115510:intron 55.0%
TTCCAGGGAGATGCTGACAG+TGG - chr7_3:62960767-62960786 Msa1115510:CDS 55.0%
TTCCACTGTCAGCATCTCCC+TGG + chr7_3:62960772-62960791 Msa1115500:intron 55.0%
TGCGCGAGTGAAGCCACAGA+GGG + chr7_3:62959530-62959549 Msa1115500:intron 60.0%
GCACGGACACTCCTCGGATT+AGG - chr7_3:62959851-62959870 Msa1115510:intron 60.0%
ACCGGGACAGACCTAATCCG+AGG + chr7_3:62959865-62959884 Msa1115500:intron 60.0%
TGGATATGACCAGCGGGGCA+GGG + chr7_3:62961040-62961059 Msa1115500:intron 60.0%
ATGGATATGACCAGCGGGGC+AGG + chr7_3:62961041-62961060 Msa1115500:intron 60.0%
TGCGCGAGTGAAGCCACAGA+GGG + chr7_3:62959530-62959549 Msa1115500:intron 60.0%
GCACGGACACTCCTCGGATT+AGG - chr7_3:62959851-62959870 Msa1115510:intron 60.0%
ACCGGGACAGACCTAATCCG+AGG + chr7_3:62959865-62959884 Msa1115500:intron 60.0%
TGGATATGACCAGCGGGGCA+GGG + chr7_3:62961040-62961059 Msa1115500:intron 60.0%
ATGGATATGACCAGCGGGGC+AGG + chr7_3:62961041-62961060 Msa1115500:intron 60.0%
GTGCGCGAGTGAAGCCACAG+AGG + chr7_3:62959531-62959550 Msa1115500:intron 65.0%
CTGTGGCTTCACTCGCGCAC+AGG - chr7_3:62959531-62959550 Msa1115510:intron 65.0%
ACCAACGCGTGTCCGACACC+GGG + chr7_3:62959882-62959901 Msa1115500:intron 65.0%
CACCAACGCGTGTCCGACAC+CGG + chr7_3:62959883-62959902 Msa1115500:intron 65.0%
TTATCACTGCCCTGCCCCGC+TGG - chr7_3:62961028-62961047 Msa1115510:five_prime_UTR 65.0%
GTGCGCGAGTGAAGCCACAG+AGG + chr7_3:62959531-62959550 Msa1115500:intron 65.0%
CTGTGGCTTCACTCGCGCAC+AGG - chr7_3:62959531-62959550 Msa1115510:intron 65.0%
ACCAACGCGTGTCCGACACC+GGG + chr7_3:62959882-62959901 Msa1115500:intron 65.0%
CACCAACGCGTGTCCGACAC+CGG + chr7_3:62959883-62959902 Msa1115500:intron 65.0%
TTATCACTGCCCTGCCCCGC+TGG - chr7_3:62961028-62961047 Msa1115510:five_prime_UTR 65.0%
! TCCCGGTGTCGGACACGCGT+TGG - chr7_3:62959878-62959897 Msa1115510:intron 70.0%
! TCCCGGTGTCGGACACGCGT+TGG - chr7_3:62959878-62959897 Msa1115510:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 62959141 62961066 62959141 ID=Msa1115510;Name=Msa1115510
chr7_3 mRNA 62959141 62961066 62959141 ID=Msa1115510-mRNA-1;Parent=Msa1115510;Name=Msa1115510-mRNA-1;_AED=0.07;_eAED=0.07;_QI=82|1|1|1|1|1|3|231|105
chr7_3 exon 62959141 62959434 62959141 ID=Msa1115510-mRNA-1:exon:14615;Parent=Msa1115510-mRNA-1
chr7_3 exon 62960655 62960787 62960655 ID=Msa1115510-mRNA-1:exon:14614;Parent=Msa1115510-mRNA-1
chr7_3 exon 62960863 62961066 62960863 ID=Msa1115510-mRNA-1:exon:14613;Parent=Msa1115510-mRNA-1
chr7_3 five_prime_UTR 62960985 62961066 62960985 ID=Msa1115510-mRNA-1:five_prime_utr;Parent=Msa1115510-mRNA-1
chr7_3 CDS 62960863 62960984 62960863 ID=Msa1115510-mRNA-1:cds;Parent=Msa1115510-mRNA-1
chr7_3 CDS 62960655 62960787 62960655 ID=Msa1115510-mRNA-1:cds;Parent=Msa1115510-mRNA-1
chr7_3 CDS 62959372 62959434 62959372 ID=Msa1115510-mRNA-1:cds;Parent=Msa1115510-mRNA-1
chr7_3 three_prime_UTR 62959141 62959371 62959141 ID=Msa1115510-mRNA-1:three_prime_utr;Parent=Msa1115510-mRNA-1
Gene Sequence

>Msa1115510

ATGGGATTTTATGTTGATTTTTCTACGATTGTGTGTTTTGCGAAGGTGATTGATTCAGAAGAAACCCTAAAAATGCAGAACGAAGAAGGCCAAATCACCGAGCTCTACATTCCCAGAAAATGTTCTGCTACAAACAGATTGATTACTGCCAAGGATCACGCTTCGGTTCAGCTTAACATCGGTCATTTGGATGAAAGCGGTGTCTACAATGGTCAATTTTCCACTTTTGCCCTCTGTGGCTTCACTCGCGCACAGGGAGATGCTGACAGTGGAATTGATCGCTTGTGGCAGAAAAAGAAAGCTGAAATTAAGCAATAG
Protein sequence

>Msa1115510

MGFYVDFSTIVCFAKVIDSEETLKMQNEEGQITELYIPRKCSATNRLITAKDHASVQLNIGHLDESGVYNGQFSTFALCGFTRAQGDADSGIDRLWQKKKAEIKQ*