AlfalfaGEDB | Msa1066630

M. sativa cultivar zhongmu-4 / Msa1066630

KEGG: K09377
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0030029 GO:0030036 GO:0044085 GO:0044877 GO:0051015 GO:0051017 GO:0061572 GO:0071840 GO:0097435
PFAM: PF00412.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066630	XP_003623892.1	97.642	212	5	0	1	212	1	212	1.17e-151	429

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066630	sp\|O80839\|PLI2A_ARATH	70.192	208	62	0	1	208	1	208	3.97e-105	305

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066630	G7L3J0	97.642	212	5	0	1	212	1	212	5.59e-152	429

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TFs/TRs:

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Gene ID	Type	Classification
Msa1066630	TF	LIM

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1066630	Msa1155400	0.925114	2.841428e-90	-8.615850e-47
Msa1066630	Msa1155490	0.901200	3.408515e-78	-8.615850e-47
Msa1066630	Msa1155870	0.942635	5.063867e-102	-8.615850e-47
Msa1066630	Msa1305370	0.932108	1.395502e-94	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066630	MtrunA17_Chr7g0247111	97.642	212	5	0	1	212	1	212	1.08e-155	429
Msa1066630	MtrunA17_Chr8g0349391	77.295	207	36	4	1	199	1	204	8.71e-111	315
Msa1066630	MtrunA17_Chr3g0113051	61.053	190	70	2	1	186	1	190	4.84e-86	252
Msa1066630	MtrunA17_Chr8g0386501	52.830	212	96	2	1	212	1	208	9.21e-74	222
Msa1066630	MtrunA17_Chr1g0152431	50.857	175	82	2	1	171	1	175	1.43e-60	188
Msa1066630	MtrunA17_Chr4g0059371	47.977	173	83	2	2	169	3	173	3.00e-56	176
Msa1066630	MtrunA17_Chr4g0059371	43.056	72	41	0	3	74	103	174	5.72e-15	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1066630	AT2G45800.1	70.192	208	62	0	1	208	1	208	4.03e-106	305
Msa1066630	AT1G01780.1	71.144	201	53	2	1	198	1	199	5.97e-106	303
Msa1066630	AT3G61230.1	69.811	212	59	3	2	209	3	213	1.83e-104	300
Msa1066630	AT2G39900.1	64.286	182	62	1	1	179	1	182	4.19e-90	263
Msa1066630	AT3G55770.3	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1066630	AT3G55770.5	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1066630	AT3G55770.2	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1066630	AT3G55770.1	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1066630	AT3G55770.7	54.167	216	63	2	1	180	1	216	3.34e-81	242
Msa1066630	AT2G45800.2	66.258	163	55	0	46	208	15	177	5.19e-75	225

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Find 44 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAAAATGTCATTCAC+AGG	0.544997	7_2:-60706103	None:intergenic
AACATAGAGGAGAGTCTACT+GGG	0.541859	7_2:+60704919	None:intergenic
AACTGTTTATCCTTTGGAAA+AGG	0.417247	7_2:-60704860	Msa1066630:intron
AATTTCAGCAAGAACTTTCA+AGG	0.447985	7_2:-60705396	Msa1066630:intron
ACAGGCAGAAGAAAAGTCTG+AGG	0.636569	7_2:-60704184	Msa1066630:CDS
ACCACCTGAACCAGCTGATG+AGG	0.566047	7_2:-60704238	Msa1066630:CDS
ACCAGAAGAAACTGAGGAAC+AGG	0.395424	7_2:-60704202	Msa1066630:CDS
ACTTGCTTTAGGTGTGCTCA+TGG	0.497010	7_2:-60704402	Msa1066630:CDS
AGACTCTCCTCTATGTTCAG+TGG	0.619328	7_2:-60704913	Msa1066630:CDS
AGCTGGTTCAGGTGGTGGAG+TGG	0.554754	7_2:+60704245	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTTGTGTATAAAGGATATC+CGG	+	chr7_2:60704481-60704500	None:intergenic	30.0%
CTTTATACACAAGTTTGGTA+GGG	-	chr7_2:60704487-60704506	Msa1066630:intron	30.0%
TCTAAACTTGTGTCTAAGTT+GGG	-	chr7_2:60704751-60704770	Msa1066630:intron	30.0%
CCTTGAAAAGTTGTTCAAAA+TGG	+	chr7_2:60704840-60704859	None:intergenic	30.0%
AATTTCAGCAAGAACTTTCA+AGG	-	chr7_2:60704868-60704887	Msa1066630:CDS	30.0%
CACAAAAACTGTTTATCCTT+TGG	-	chr7_2:60705398-60705417	Msa1066630:CDS	30.0%
AGTAATCTCAACCATTCATA+AGG	-	chr7_2:60705528-60705547	Msa1066630:intron	30.0%
ACTCGATTGAAAATAGTGTT+AGG	-	chr7_2:60705592-60705611	Msa1066630:intron	30.0%
CTCGATTGAAAATAGTGTTA+GGG	-	chr7_2:60705593-60705612	Msa1066630:intron	30.0%
CTTTGTGAAATAGATGTCAT+TGG	-	chr7_2:60705819-60705838	Msa1066630:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	60704170	60706116	60704170	ID=Msa1066630;Name=Msa1066630
chr7_2	mRNA	60704170	60706116	60704170	ID=Msa1066630-mRNA-1;Parent=Msa1066630;Name=Msa1066630-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|1\|1\|5\|0\|212
chr7_2	exon	60705982	60706116	60705982	ID=Msa1066630-mRNA-1:exon:12023;Parent=Msa1066630-mRNA-1
chr7_2	exon	60705397	60705493	60705397	ID=Msa1066630-mRNA-1:exon:12022;Parent=Msa1066630-mRNA-1
chr7_2	exon	60705207	60705238	60705207	ID=Msa1066630-mRNA-1:exon:12021;Parent=Msa1066630-mRNA-1
chr7_2	exon	60704861	60704950	60704861	ID=Msa1066630-mRNA-1:exon:12020;Parent=Msa1066630-mRNA-1
chr7_2	exon	60704170	60704454	60704170	ID=Msa1066630-mRNA-1:exon:12019;Parent=Msa1066630-mRNA-1
chr7_2	CDS	60705982	60706116	60705982	ID=Msa1066630-mRNA-1:cds;Parent=Msa1066630-mRNA-1
chr7_2	CDS	60705397	60705493	60705397	ID=Msa1066630-mRNA-1:cds;Parent=Msa1066630-mRNA-1
chr7_2	CDS	60705207	60705238	60705207	ID=Msa1066630-mRNA-1:cds;Parent=Msa1066630-mRNA-1
chr7_2	CDS	60704861	60704950	60704861	ID=Msa1066630-mRNA-1:cds;Parent=Msa1066630-mRNA-1
chr7_2	CDS	60704170	60704454	60704170	ID=Msa1066630-mRNA-1:cds;Parent=Msa1066630-mRNA-1

Gene Sequence

>Msa1066630

ATGTCATTCACAGGAACTCTTGATAAATGCAAGGCTTGTGACAAGACTGTTTATGTGGTTGATTTGTTAACTCTTGAAGGTATACCTTACCATAAAAGTTGCTTAAAATGCACTCACTGCAAGGGAAATCTTACGATGAGTACTTACTCGTCAATGGATGGTGTCCTCTATTGCAAGCCCCATTTTGAACAACTTTTCAAGGAATCTGGAAATTTCAGCAAGAACTTTCAAGCAAAGTCTTCTGAGAAGATAAATGAGTTGATGAACAGGACACCCAGTAGACTCTCCTCTATGTTCAGTGGAACTTTAGACAAATGTGCAGTTTGCACAAAAACTGTTTATCCTTTGGAAAAGATGTCATTGGAAGGGGAATGTTACCACAAGACTTGCTTTAGGTGTGCTCATGGAGGTTGCCACCTTACACATTCTTCCTATGCTGCACTAGATGGTGTCCTCTATTGCAAGCATCATTTTCAACAGCTTTTCATGGAGAAAGGAAACTACAATCATGTGCTTCAAGCTGCTGCAAACAAAAAGAATTCCACTCCACCACCTGAACCAGCTGATGAGGATGAGGCATCAAAACCAGAAGAAACTGAGGAACAGGCAGAAGAAAAGTCTGAGGCAGCAGAAACATGA

Protein sequence

>Msa1066630

MSFTGTLDKCKACDKTVYVVDLLTLEGIPYHKSCLKCTHCKGNLTMSTYSSMDGVLYCKPHFEQLFKESGNFSKNFQAKSSEKINELMNRTPSRLSSMFSGTLDKCAVCTKTVYPLEKMSLEGECYHKTCFRCAHGGCHLTHSSYAALDGVLYCKHHFQQLFMEKGNYNHVLQAAANKKNSTPPPEPADEDEASKPEETEEQAEEKSEAAET*