AlfalfaGEDB | Msa1305370

M. sativa cultivar zhongmu-4 / Msa1305370

KEGG: K09377
GO: GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0030029 GO:0030036 GO:0044085 GO:0044877 GO:0051015 GO:0051017 GO:0061572 GO:0071840 GO:0097435
PFAM: PF00412.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305370	XP_003623892.1	97.642	212	5	0	1	212	1	212	1.17e-151	429

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305370	sp\|O80839\|PLI2A_ARATH	70.192	208	62	0	1	208	1	208	3.97e-105	305

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305370	G7L3J0	97.642	212	5	0	1	212	1	212	5.59e-152	429

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TFs/TRs:

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Gene ID	Type	Classification
Msa1305370	TF	LIM

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1066630	Msa1305370	0.932108	1.395502e-94	-8.615850e-47
Msa1155400	Msa1305370	0.965778	4.766326e-125	-8.615850e-47
Msa1155490	Msa1305370	0.913892	3.631293e-84	-8.615850e-47
Msa1155870	Msa1305370	0.971613	1.933082e-133	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305370	MtrunA17_Chr7g0247111	97.642	212	5	0	1	212	1	212	1.08e-155	429
Msa1305370	MtrunA17_Chr8g0349391	77.295	207	36	4	1	199	1	204	8.71e-111	315
Msa1305370	MtrunA17_Chr3g0113051	61.053	190	70	2	1	186	1	190	4.84e-86	252
Msa1305370	MtrunA17_Chr8g0386501	52.830	212	96	2	1	212	1	208	9.21e-74	222
Msa1305370	MtrunA17_Chr1g0152431	50.857	175	82	2	1	171	1	175	1.43e-60	188
Msa1305370	MtrunA17_Chr4g0059371	47.977	173	83	2	2	169	3	173	3.00e-56	176
Msa1305370	MtrunA17_Chr4g0059371	43.056	72	41	0	3	74	103	174	5.72e-15	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1305370	AT2G45800.1	70.192	208	62	0	1	208	1	208	4.03e-106	305
Msa1305370	AT1G01780.1	71.144	201	53	2	1	198	1	199	5.97e-106	303
Msa1305370	AT3G61230.1	69.811	212	59	3	2	209	3	213	1.83e-104	300
Msa1305370	AT2G39900.1	64.286	182	62	1	1	179	1	182	4.19e-90	263
Msa1305370	AT3G55770.3	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1305370	AT3G55770.5	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1305370	AT3G55770.2	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1305370	AT3G55770.1	64.286	182	63	1	1	180	1	182	3.54e-89	261
Msa1305370	AT3G55770.7	54.167	216	63	2	1	180	1	216	3.34e-81	242
Msa1305370	AT2G45800.2	66.258	163	55	0	46	208	15	177	5.19e-75	225

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Find 44 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAAAATGTCATTCAC+AGG	0.544997	8_3:-68831156	None:intergenic
AACATAGAGGAGAGTCTACT+GGG	0.541859	8_3:+68829972	None:intergenic
AACTGTTTATCCTTTGGAAA+AGG	0.417247	8_3:-68829913	Msa1305370:intron
AATTTCAGCAAGAACTTTCA+AGG	0.447985	8_3:-68830449	Msa1305370:intron
ACAGGCAGAAGAAAAGTCTG+AGG	0.636569	8_3:-68829241	Msa1305370:CDS
ACCACCTGAACCAGCTGATG+AGG	0.566047	8_3:-68829295	Msa1305370:CDS
ACCAGAAGAAACTGAGGAAC+AGG	0.395424	8_3:-68829259	Msa1305370:CDS
ACTTGCTTTAGGTGTGCTCA+TGG	0.497010	8_3:-68829459	Msa1305370:CDS
AGACTCTCCTCTATGTTCAG+TGG	0.619328	8_3:-68829966	Msa1305370:CDS
AGCTGGTTCAGGTGGTGGAG+TGG	0.554754	8_3:+68829302	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTTGTGTATAAAGGATATC+CGG	+	chr8_3:68829538-68829557	None:intergenic	30.0%
CTTTATACACAAGTTTGGTA+GGG	-	chr8_3:68829544-68829563	Msa1305370:intron	30.0%
TCTAAACTTGTGTCTAAGTT+GGG	-	chr8_3:68829808-68829827	Msa1305370:intron	30.0%
CCTTGAAAAGTTGTTCAAAA+TGG	+	chr8_3:68829897-68829916	None:intergenic	30.0%
AATTTCAGCAAGAACTTTCA+AGG	-	chr8_3:68829925-68829944	Msa1305370:CDS	30.0%
CACAAAAACTGTTTATCCTT+TGG	-	chr8_3:68830455-68830474	Msa1305370:CDS	30.0%
AGTAATCTCAACCATTCATA+AGG	-	chr8_3:68830585-68830604	Msa1305370:intron	30.0%
ACTCGATTGAAAATAGTGTT+AGG	-	chr8_3:68830649-68830668	Msa1305370:intron	30.0%
CTCGATTGAAAATAGTGTTA+GGG	-	chr8_3:68830650-68830669	Msa1305370:intron	30.0%
CTTTGTGAAATAGATGTCAT+TGG	-	chr8_3:68830872-68830891	Msa1305370:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	68829227	68831169	68829227	ID=Msa1305370;Name=Msa1305370
chr8_3	mRNA	68829227	68831169	68829227	ID=Msa1305370-mRNA-1;Parent=Msa1305370;Name=Msa1305370-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|1\|1\|5\|0\|212
chr8_3	exon	68831035	68831169	68831035	ID=Msa1305370-mRNA-1:exon:21331;Parent=Msa1305370-mRNA-1
chr8_3	exon	68830450	68830546	68830450	ID=Msa1305370-mRNA-1:exon:21330;Parent=Msa1305370-mRNA-1
chr8_3	exon	68830260	68830291	68830260	ID=Msa1305370-mRNA-1:exon:21329;Parent=Msa1305370-mRNA-1
chr8_3	exon	68829914	68830003	68829914	ID=Msa1305370-mRNA-1:exon:21328;Parent=Msa1305370-mRNA-1
chr8_3	exon	68829227	68829511	68829227	ID=Msa1305370-mRNA-1:exon:21327;Parent=Msa1305370-mRNA-1
chr8_3	CDS	68831035	68831169	68831035	ID=Msa1305370-mRNA-1:cds;Parent=Msa1305370-mRNA-1
chr8_3	CDS	68830450	68830546	68830450	ID=Msa1305370-mRNA-1:cds;Parent=Msa1305370-mRNA-1
chr8_3	CDS	68830260	68830291	68830260	ID=Msa1305370-mRNA-1:cds;Parent=Msa1305370-mRNA-1
chr8_3	CDS	68829914	68830003	68829914	ID=Msa1305370-mRNA-1:cds;Parent=Msa1305370-mRNA-1
chr8_3	CDS	68829227	68829511	68829227	ID=Msa1305370-mRNA-1:cds;Parent=Msa1305370-mRNA-1

Gene Sequence

>Msa1305370

ATGTCATTCACAGGAACTCTTGATAAATGCAAGGCTTGTGACAAGACTGTTTATGTGGTTGATTTGTTAACTCTTGAAGGTATACCTTACCATAAAAGTTGCTTAAAATGCACTCACTGCAAGGGAAATCTTACGATGAGTACTTACTCGTCAATGGATGGTGTCCTCTATTGCAAGCCCCATTTTGAACAACTTTTCAAGGAATCTGGAAATTTCAGCAAGAACTTTCAAGCAAAGTCTTCTGAGAAGATAAATGAGTTGATGAACAGGACACCCAGTAGACTCTCCTCTATGTTCAGTGGAACTTTAGACAAATGTGCAGTTTGCACAAAAACTGTTTATCCTTTGGAAAAGATGTCATTGGAAGGGGAATGTTACCACAAGACTTGCTTTAGGTGTGCTCATGGAGGTTGCCACCTTACACATTCTTCCTATGCTGCACTAGATGGTGTCCTCTATTGCAAGCATCATTTTCAACAGCTTTTCATGGAGAAAGGAAACTACAATCATGTGCTTCAAGCTGCTGCAAACAAAAAGAATTCCACTCCACCACCTGAACCAGCTGATGAGGATGAGGCATCAAAACCAGAAGAAACTGAGGAACAGGCAGAAGAAAAGTCTGAGGCAGCAGAAACATGA

Protein sequence

>Msa1305370

MSFTGTLDKCKACDKTVYVVDLLTLEGIPYHKSCLKCTHCKGNLTMSTYSSMDGVLYCKPHFEQLFKESGNFSKNFQAKSSEKINELMNRTPSRLSSMFSGTLDKCAVCTKTVYPLEKMSLEGECYHKTCFRCAHGGCHLTHSSYAALDGVLYCKHHFQQLFMEKGNYNHVLQAAANKKNSTPPPEPADEDEASKPEETEEQAEEKSEAAET*