AlfalfaGEDB | Msa1096850

M. sativa cultivar zhongmu-4 / Msa1096850

KEGG: K16297
GO: GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009505 GO:0009506 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030054 GO:0030163 GO:0030312 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0051603 GO:0055044 GO:0070008 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901565 GO:1901575
PFAM: PF00450.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1096850	XP_003621641.2	92.531	482	14	1	1	482	1	460	0.0	921

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1096850	sp\|Q93Y09\|SCP45_ARATH	75.877	456	88	1	27	482	28	461	0.0	728

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1096850	G7KSX5	92.531	482	14	1	1	482	1	460	0.0	921

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017950	Msa1096850	0.809093	2.243876e-50	-8.615850e-47
Msa0548470	Msa1096850	0.810573	1.077680e-50	-8.615850e-47
Msa0581470	Msa1096850	0.807571	4.735947e-50	-8.615850e-47
Msa0644410	Msa1096850	0.804214	2.405730e-49	-8.615850e-47
Msa1011530	Msa1096850	0.837185	6.033766e-57	-8.615850e-47
Msa1051900	Msa1096850	0.882483	1.003257e-70	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1096850	MtrunA17_Chr7g0221551	92.531	482	14	1	1	482	1	460	0.0	921
Msa1096850	MtrunA17_Chr3g0131731	66.667	486	128	5	9	482	1	464	0.0	669
Msa1096850	MtrunA17_Chr8g0385871	65.217	483	135	4	8	482	8	465	0.0	660
Msa1096850	MtrunA17_Chr3g0094811	60.965	456	153	2	24	479	24	454	0.0	590
Msa1096850	MtrunA17_Chr2g0304931	56.427	459	169	6	24	479	32	462	0.0	519
Msa1096850	MtrunA17_Chr1g0204191	46.491	456	192	6	26	480	87	491	6.79e-142	416
Msa1096850	MtrunA17_Chr3g0126051	45.633	458	214	7	31	480	34	464	1.12e-140	412
Msa1096850	MtrunA17_Chr5g0431301	44.518	456	222	7	31	479	30	461	5.16e-135	398
Msa1096850	MtrunA17_Chr3g0126031	44.298	456	219	8	31	479	29	456	1.15e-134	397
Msa1096850	MtrunA17_Chr7g0219051	40.987	466	227	9	29	480	34	465	3.08e-115	347

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1096850	AT1G28110.1	75.877	456	88	1	27	482	28	461	0.0	728
Msa1096850	AT1G28110.2	75.877	456	88	1	27	482	28	461	0.0	728
Msa1096850	AT2G33530.1	67.967	487	129	3	1	482	1	465	0.0	701
Msa1096850	AT1G43780.1	47.609	460	207	8	31	481	38	472	8.49e-144	421
Msa1096850	AT2G12480.3	43.354	489	235	11	5	481	1	459	4.51e-134	395
Msa1096850	AT5G42240.1	44.635	466	225	8	23	479	23	464	2.76e-133	394
Msa1096850	AT5G42230.1	42.469	478	242	8	13	481	9	462	5.28e-132	390
Msa1096850	AT5G42230.2	42.469	478	242	8	13	481	9	462	5.28e-132	390
Msa1096850	AT2G12480.4	42.300	487	219	11	5	481	1	435	3.08e-127	377
Msa1096850	AT2G12480.1	41.684	487	220	11	5	481	1	433	1.44e-124	370

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Find 96 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAAGACAAATCTTTA+AGG	0.238128	7_3:-21923570	None:intergenic
AAAGAGAATTACCTGTTGCA+GGG	0.615032	7_3:-21925125	None:intergenic
AAAGTTCTACTTCCAGTCAA+TGG	0.487618	7_3:-21926227	None:intergenic
AAATCAACATGTGGTTGACC+AGG	0.571157	7_3:-21922264	None:intergenic
AAATGTAACATGACAGGGAA+AGG	0.562297	7_3:-21926761	None:intergenic
AACACTAATCAAAATTCTTC+TGG	0.409071	7_3:-21926642	None:intergenic
AACAGAAGCTTGTTCATCGT+AGG	0.672452	7_3:+21923676	Msa1096850:CDS
AACAGCACAAGCGATCTCTC+AGG	0.518018	7_3:-21926591	None:intergenic
AACCAAAGTCCTCAATACTC+AGG	0.621880	7_3:+21924691	Msa1096850:CDS
AACCAGTTCAGCTGGAACAC+AGG	0.532001	7_3:+21922579	Msa1096850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTGATGAAAATCAACATG+TGG	-	chr7_3:21922276-21922295	None:intergenic	30.0%
AGACTGTTTGGAAGAATTTA+TGG	+	chr7_3:21922732-21922751	Msa1096850:intron	30.0%
AACTTTATGGAAACGACTTA+TGG	-	chr7_3:21922776-21922795	None:intergenic	30.0%
TCCAATTTCCATTAATGTGT+TGG	+	chr7_3:21923090-21923109	Msa1096850:intron	30.0%
ACCAACACATTAATGGAAAT+TGG	-	chr7_3:21923094-21923113	None:intergenic	30.0%
CAATTCACATTTACACACAA+TGG	-	chr7_3:21923150-21923169	None:intergenic	30.0%
TAAGAAAATCCTACTCCAAT+TGG	-	chr7_3:21923202-21923221	None:intergenic	30.0%
AAAACAAGACTATTATGCAG+TGG	-	chr7_3:21923530-21923549	None:intergenic	30.0%
TGTACCTTAAAGATTTGTCT+TGG	+	chr7_3:21923566-21923585	Msa1096850:CDS	30.0%
ATCTAATCTTCTTGCAAAAC+TGG	+	chr7_3:21923629-21923648	Msa1096850:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	21922126	21926857	21922126	ID=Msa1096850;Name=Msa1096850
chr7_3	mRNA	21922126	21926857	21922126	ID=Msa1096850-mRNA-1;Parent=Msa1096850;Name=Msa1096850-mRNA-1;_AED=0.24;_eAED=0.24;_QI=36\|0.88\|0.8\|1\|1\|1\|10\|201\|482
chr7_3	exon	21922126	21922405	21922126	ID=Msa1096850-mRNA-1:exon:4784;Parent=Msa1096850-mRNA-1
chr7_3	exon	21922499	21922600	21922499	ID=Msa1096850-mRNA-1:exon:4785;Parent=Msa1096850-mRNA-1
chr7_3	exon	21923173	21923272	21923173	ID=Msa1096850-mRNA-1:exon:4786;Parent=Msa1096850-mRNA-1
chr7_3	exon	21923558	21923712	21923558	ID=Msa1096850-mRNA-1:exon:4787;Parent=Msa1096850-mRNA-1
chr7_3	exon	21924018	21924097	21924018	ID=Msa1096850-mRNA-1:exon:4788;Parent=Msa1096850-mRNA-1
chr7_3	exon	21924431	21924712	21924431	ID=Msa1096850-mRNA-1:exon:4789;Parent=Msa1096850-mRNA-1
chr7_3	exon	21925011	21925135	21925011	ID=Msa1096850-mRNA-1:exon:4790;Parent=Msa1096850-mRNA-1
chr7_3	exon	21925573	21925665	21925573	ID=Msa1096850-mRNA-1:exon:4791;Parent=Msa1096850-mRNA-1
chr7_3	exon	21926205	21926322	21926205	ID=Msa1096850-mRNA-1:exon:4792;Parent=Msa1096850-mRNA-1
chr7_3	exon	21926507	21926857	21926507	ID=Msa1096850-mRNA-1:exon:4793;Parent=Msa1096850-mRNA-1
chr7_3	five_prime_UTR	21922126	21922161	21922126	ID=Msa1096850-mRNA-1:five_prime_utr;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21922162	21922405	21922162	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21922499	21922600	21922499	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21923173	21923272	21923173	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21923558	21923712	21923558	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21924018	21924097	21924018	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21924431	21924712	21924431	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21925011	21925135	21925011	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21925573	21925665	21925573	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21926205	21926322	21926205	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	CDS	21926507	21926656	21926507	ID=Msa1096850-mRNA-1:cds;Parent=Msa1096850-mRNA-1
chr7_3	three_prime_UTR	21926657	21926857	21926657	ID=Msa1096850-mRNA-1:three_prime_utr;Parent=Msa1096850-mRNA-1

Gene Sequence

>Msa1096850

ATGTCTTCACTACTTTGTTGTTCCATTGCAATGTGTGTTGCTGCAATTGTACTTCAACAATGTTCCTTTTCAGTTTCACACCCTAATAAGATCACAAATTTACCTGGTCAACCACATGTTGATTTTCATCAGTTTTCAGGCTATGTAAATGTTGATGATCAAAACAAAAAAGCACTCTTTTTCTACTTTGTTGAAGCTCAGAATGATGATGTTTCCAAACCTCTTGTTCTTTGGCTCAATGGAGGGCCTGGTTGTTCTTCTCTTGGTGTTGGTGCATTTTCAGAAAATGGGCCATTTAGGCCTAAAGGAGAGGCTTTGGTTAGGAACCAGTTCAGCTGGAACACAGAGGCAAATATGTTGTACTTGGAATCACCAATTGGAGTAGGATTTTCTTATTCGACCGATACTTCAACTTATGAAGGTGTAAATGACAAGATTACAGGCAAACATGTCATGTACCTTAAAGATTTGTCTTGGTTTTCTGAAATTATTGTCTTTCTCTTTATAGCCAGGGATAATCTAATCTTCTTGCAAAACTGGTTTGTGAAGTTCCCTGAATACAGAAACAGAAGCTTGTTCATCGTAGGAGAGAGCTATGCCGGTCATTATGTTCCTCAATTGGCTGAGCTTATGCTCCAATTCAACAAAAAGGAGAAGTTGTTTAATTTAAAAGGCATTGCTCTTGGTAATCCAGTTCTAGAATTTGCAACAGACTTTAATTCAAGAGCTGAGTTCTTTTGGTCTCATGGATTGATCTCAGACTTAACATTTAAAATGTTCACTTCAGTTTGTAACTATTCAAGATATGTGAGAGAATACTACAACGGTGCCGTTTCTCCCATTTGTTCAAGTGTAATGAGCCAAGTTAGTACAGAAACAAGTAGATTTGTTGACAAGTATGATGTGACCCTTGATGTTTGTATATCTTCTGTGTTTTCACAAACCAAAGTCCTCAATACTCAGCAAGTGACTGAAACGGTTGATGTATGCGTTGAAGACGAGACTGTCAATTATCTTAACAGGAAAGATGTTCAATCAGCTTTACATGCACATCTGATTGGAGTACATAGATGGTCTCCCTGCAACAGTGTGTTGGATTATGAGTTGCGTGACTTGGAGATACCAACAATCACGGTTGTCGGCAAGCTAGTCAAGGCAGGAGTTCCAGTTTTGGTCTACAGTGGTGATCAGGATTCAGTTATTCCATTGACTGGAAGTAGAACTTTAGTACATCAATTGGCAAAACAGCTGAGAATGAATACCACTGTGCCTTACAGGGTGTGGTTTGCAGGCCAACAGGTTGGTGGTTGGACTCAAGTTTATGGCAACATACTATCATTTGCAACAGTTAGGGGAGCATCACATGAAGTACCCTTTTCACAGCCTGAGAGATCGCTTGTGCTGTTTAAGTCGTTCTTAGAAGGAAGACCTCTACCAGAAGAATTTTGA

Protein sequence

>Msa1096850

MSSLLCCSIAMCVAAIVLQQCSFSVSHPNKITNLPGQPHVDFHQFSGYVNVDDQNKKALFFYFVEAQNDDVSKPLVLWLNGGPGCSSLGVGAFSENGPFRPKGEALVRNQFSWNTEANMLYLESPIGVGFSYSTDTSTYEGVNDKITGKHVMYLKDLSWFSEIIVFLFIARDNLIFLQNWFVKFPEYRNRSLFIVGESYAGHYVPQLAELMLQFNKKEKLFNLKGIALGNPVLEFATDFNSRAEFFWSHGLISDLTFKMFTSVCNYSRYVREYYNGAVSPICSSVMSQVSTETSRFVDKYDVTLDVCISSVFSQTKVLNTQQVTETVDVCVEDETVNYLNRKDVQSALHAHLIGVHRWSPCNSVLDYELRDLEIPTITVVGKLVKAGVPVLVYSGDQDSVIPLTGSRTLVHQLAKQLRMNTTVPYRVWFAGQQVGGWTQVYGNILSFATVRGASHEVPFSQPERSLVLFKSFLEGRPLPEEF*