Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1115760 | XP_003625187.2 | 95.349 | 172 | 7 | 1 | 1 | 171 | 1 | 172 | 1.83e-117 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1115760 | sp|A3BDI8|SAP8_ORYSJ | 59.649 | 171 | 64 | 2 | 4 | 171 | 3 | 171 | 3.63e-71 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1115760 | A2Q3D5 | 95.349 | 172 | 7 | 1 | 1 | 171 | 1 | 172 | 8.76e-118 | 339 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0196530 | Msa1115760 | 0.816520 | 5.301647e-52 | -8.615850e-47 |
| Msa1034870 | Msa1115760 | 0.803969 | 2.704262e-49 | -8.615850e-47 |
| Msa1074910 | Msa1115760 | 0.813806 | 2.124528e-51 | -8.615850e-47 |
| Msa1115760 | Msa1162220 | 0.869057 | 4.117964e-66 | -8.615850e-47 |
| Msa1115760 | Msa1258600 | 0.812689 | 3.737121e-51 | -8.615850e-47 |
| Msa1115760 | Msa1298440 | 0.826763 | 2.269341e-54 | -8.615850e-47 |
| Msa1115760 | Msa1400940 | 0.812653 | 3.804109e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1115760 | MtrunA17_Chr7g0258071 | 95.349 | 172 | 7 | 1 | 1 | 171 | 89 | 260 | 3.93e-121 | 342 |
| Msa1115760 | MtrunA17_Chr1g0173971 | 71.512 | 172 | 45 | 2 | 1 | 171 | 1 | 169 | 7.45e-89 | 257 |
| Msa1115760 | MtrunA17_Chr2g0328941 | 60.000 | 170 | 66 | 2 | 4 | 171 | 3 | 172 | 2.27e-72 | 215 |
| Msa1115760 | MtrunA17_Chr4g0032621 | 55.135 | 185 | 66 | 3 | 4 | 171 | 3 | 187 | 1.67e-68 | 206 |
| Msa1115760 | MtrunA17_Chr7g0266121 | 40.341 | 176 | 86 | 3 | 5 | 171 | 3 | 168 | 3.87e-43 | 141 |
| Msa1115760 | MtrunA17_Chr2g0321261 | 42.675 | 157 | 74 | 4 | 15 | 171 | 14 | 154 | 2.12e-38 | 128 |
| Msa1115760 | MtrunA17_Chr1g0178851 | 42.604 | 169 | 80 | 2 | 5 | 171 | 3 | 156 | 3.47e-38 | 128 |
| Msa1115760 | MtrunA17_Chr4g0026561 | 39.535 | 172 | 95 | 4 | 5 | 171 | 3 | 170 | 6.46e-37 | 125 |
| Msa1115760 | MtrunA17_Chr8g0351971 | 61.765 | 68 | 26 | 0 | 104 | 171 | 173 | 240 | 4.16e-27 | 102 |
| Msa1115760 | MtrunA17_Chr1g0203591 | 36.774 | 155 | 79 | 4 | 15 | 169 | 3 | 138 | 4.48e-27 | 99.4 |
| Msa1115760 | MtrunA17_Chr3g0087831 | 37.419 | 155 | 78 | 2 | 17 | 171 | 28 | 163 | 7.97e-27 | 99.4 |
| Msa1115760 | MtrunA17_Chr7g0274001 | 33.766 | 154 | 76 | 3 | 15 | 168 | 3 | 130 | 8.27e-25 | 93.2 |
| Msa1115760 | MtrunA17_Chr2g0306911 | 30.323 | 155 | 75 | 4 | 15 | 169 | 4 | 125 | 8.27e-18 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1115760 | AT1G51200.4 | 53.073 | 179 | 65 | 4 | 4 | 171 | 3 | 173 | 1.05e-51 | 163 |
| Msa1115760 | AT1G51200.1 | 53.073 | 179 | 65 | 4 | 4 | 171 | 3 | 173 | 1.05e-51 | 163 |
| Msa1115760 | AT1G51200.3 | 53.073 | 179 | 65 | 4 | 4 | 171 | 3 | 173 | 1.05e-51 | 163 |
| Msa1115760 | AT1G51200.2 | 53.073 | 179 | 65 | 4 | 4 | 171 | 3 | 173 | 1.05e-51 | 163 |
| Msa1115760 | AT2G36320.1 | 43.452 | 168 | 85 | 2 | 5 | 171 | 3 | 161 | 2.61e-44 | 144 |
| Msa1115760 | AT3G52800.2 | 42.690 | 171 | 91 | 3 | 5 | 171 | 3 | 170 | 1.52e-39 | 132 |
| Msa1115760 | AT3G52800.1 | 42.690 | 171 | 91 | 3 | 5 | 171 | 3 | 170 | 1.52e-39 | 132 |
| Msa1115760 | AT2G27580.2 | 37.500 | 168 | 84 | 3 | 13 | 171 | 8 | 163 | 1.58e-37 | 127 |
| Msa1115760 | AT2G27580.1 | 37.500 | 168 | 84 | 3 | 13 | 171 | 8 | 163 | 1.58e-37 | 127 |
| Msa1115760 | AT4G12040.2 | 36.585 | 164 | 99 | 2 | 12 | 171 | 13 | 175 | 1.95e-36 | 124 |
| Msa1115760 | AT4G12040.1 | 36.585 | 164 | 99 | 2 | 12 | 171 | 13 | 175 | 1.95e-36 | 124 |
| Msa1115760 | AT1G12440.2 | 38.788 | 165 | 89 | 2 | 11 | 171 | 12 | 168 | 2.03e-36 | 124 |
| Msa1115760 | AT1G12440.1 | 38.788 | 165 | 89 | 2 | 11 | 171 | 12 | 168 | 2.03e-36 | 124 |
| Msa1115760 | AT1G12440.3 | 38.788 | 165 | 89 | 2 | 11 | 171 | 12 | 168 | 2.03e-36 | 124 |
| Msa1115760 | AT4G22820.1 | 38.750 | 160 | 93 | 3 | 15 | 171 | 19 | 176 | 1.23e-34 | 120 |
| Msa1115760 | AT4G22820.2 | 38.750 | 160 | 93 | 3 | 15 | 171 | 19 | 176 | 1.23e-34 | 120 |
| Msa1115760 | AT3G12630.1 | 38.926 | 149 | 72 | 3 | 23 | 171 | 31 | 160 | 5.25e-31 | 110 |
| Msa1115760 | AT4G14225.1 | 35.032 | 157 | 66 | 4 | 15 | 171 | 5 | 125 | 5.16e-26 | 96.7 |
| Msa1115760 | AT4G25380.1 | 31.013 | 158 | 72 | 3 | 16 | 171 | 8 | 130 | 1.29e-24 | 93.2 |
Find 44 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTAATCCAACACGTTTC+CGG | 0.268508 | 7_3:-63283924 | None:intergenic |
| AGTTTATCCGCCTTAATTAC+TGG | 0.271098 | 7_3:-63284060 | None:intergenic |
| CGGAAACGTGTTGGATTAAC+TGG | 0.273573 | 7_3:+63283925 | Msa1115760:CDS |
| TGAATCCAACCCAGTAATTA+AGG | 0.350084 | 7_3:+63284050 | Msa1115760:CDS |
| CAATTATTAACGCACAGAAT+TGG | 0.359796 | 7_3:-63283622 | None:intergenic |
| ATCTCCATCTCATTAGAGAT+TGG | 0.379259 | 7_3:-63283865 | None:intergenic |
| CCAGCAGATGCACTACTTGC+CGG | 0.396434 | 7_3:+63283905 | Msa1115760:CDS |
| GAATTGGACGTTCAGGTGTC+TGG | 0.402600 | 7_3:-63283606 | None:intergenic |
| GTTAATAATTGTGGTTTCTT+CGG | 0.432078 | 7_3:+63283634 | Msa1115760:CDS |
| GCATCTGCTGGGACCAGTCT+TGG | 0.436198 | 7_3:-63283893 | None:intergenic |
| CCGGCAAGTAGTGCATCTGC+TGG | 0.450708 | 7_3:-63283905 | None:intergenic |
| ACGCACAGAATTGGACGTTC+AGG | 0.451830 | 7_3:-63283613 | None:intergenic |
| CCAGCAGTTCTATAATCAAA+AGG | 0.452505 | 7_3:-63284012 | None:intergenic |
| ATCCGCCTTAATTACTGGGT+TGG | 0.457754 | 7_3:-63284055 | None:intergenic |
| TTCCAGCGGCAATGGAAAGC+TGG | 0.475284 | 7_3:+63283765 | Msa1115760:CDS |
| AATGGCAATTCCAGCGGCAA+TGG | 0.485507 | 7_3:+63283757 | Msa1115760:CDS |
| GTTTATCCGCCTTAATTACT+GGG | 0.487636 | 7_3:-63284059 | None:intergenic |
| AGCCAGCTTTCCATTGCCGC+TGG | 0.489376 | 7_3:-63283767 | None:intergenic |
| CATGTGTTCCAAGTGTTACA+AGG | 0.489662 | 7_3:+63283675 | Msa1115760:CDS |
| ACACTTGGAACACATGTTCA+TGG | 0.501814 | 7_3:-63283668 | None:intergenic |
| TACTGGGTTGGATTCAGCTA+TGG | 0.503220 | 7_3:-63284043 | None:intergenic |
| AATGGAGTCTCATGATGAGA+TGG | 0.509058 | 7_3:+63283579 | None:intergenic |
| ATAATTGTGGTTTCTTCGGA+AGG | 0.510786 | 7_3:+63283638 | Msa1115760:CDS |
| ATTCTGTGCGTTAATAATTG+TGG | 0.511147 | 7_3:+63283625 | Msa1115760:CDS |
| CAGCAGTTCTATAATCAAAA+GGG | 0.523968 | 7_3:-63284011 | None:intergenic |
| CAGTGTGTCCTTGTAACACT+TGG | 0.534504 | 7_3:-63283683 | None:intergenic |
| AGCGATGCATTGAGCAAAAG+AGG | 0.535711 | 7_3:-63283969 | None:intergenic |
| TCAGTTGAAAACATCGTGAA+TGG | 0.542840 | 7_3:+63283739 | Msa1115760:CDS |
| CAAGGACACACTGCTGAAGC+AGG | 0.543963 | 7_3:+63283693 | Msa1115760:CDS |
| ATGGAGATGAAAGCCAAGAC+TGG | 0.550708 | 7_3:+63283880 | Msa1115760:CDS |
| AAATCCAATCTCTAATGAGA+TGG | 0.551991 | 7_3:+63283861 | Msa1115760:CDS |
| CACACTGCTGAAGCAGGAGC+AGG | 0.565527 | 7_3:+63283699 | Msa1115760:CDS |
| CGGCAAGTAGTGCATCTGCT+GGG | 0.567957 | 7_3:-63283904 | None:intergenic |
| ACTGGTTTCAGCTGCAAATG+TGG | 0.568603 | 7_3:+63283943 | Msa1115760:CDS |
| TTATAGAACTGCTGGTCAGA+AGG | 0.572119 | 7_3:+63284020 | Msa1115760:CDS |
| ACTACTTGCCGGAAACGTGT+TGG | 0.596630 | 7_3:+63283916 | Msa1115760:CDS |
| TAATTGTGGTTTCTTCGGAA+GGG | 0.609724 | 7_3:+63283639 | Msa1115760:CDS |
| GAAAGCTGGCTATGACCGCA+AGG | 0.621304 | 7_3:+63283779 | Msa1115760:CDS |
| ATGGAGTCTCATGATGAGAT+GGG | 0.645569 | 7_3:+63283580 | Msa1115760:CDS |
| ATCGTGAATGGCAATTCCAG+CGG | 0.654212 | 7_3:+63283751 | Msa1115760:CDS |
| TCGCACATCAACAGTCCTTG+CGG | 0.667104 | 7_3:-63283794 | None:intergenic |
| GCTCAATACAGTGTCTCCCG+AGG | 0.676644 | 7_3:+63283831 | Msa1115760:CDS |
| ATCCAACCCAGTAATTAAGG+CGG | 0.681546 | 7_3:+63284053 | Msa1115760:CDS |
| TGATGTGCGAGTTGAAAGTG+TGG | 0.723729 | 7_3:+63283807 | Msa1115760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTAATAATTGTGGTTTCTT+CGG | + | chr7_3:63283634-63283653 | Msa1115760:CDS | 25.0% |
| CAATTATTAACGCACAGAAT+TGG | - | chr7_3:63283625-63283644 | None:intergenic | 30.0% | |
| ATTCTGTGCGTTAATAATTG+TGG | + | chr7_3:63283625-63283644 | Msa1115760:CDS | 30.0% | |
| ! | TCATTAGAGATTGGATTTTC+TGG | - | chr7_3:63283859-63283878 | None:intergenic | 30.0% |
| AAATCCAATCTCTAATGAGA+TGG | + | chr7_3:63283861-63283880 | Msa1115760:CDS | 30.0% | |
| CAGCAGTTCTATAATCAAAA+GGG | - | chr7_3:63284014-63284033 | None:intergenic | 30.0% | |
| ATAATTGTGGTTTCTTCGGA+AGG | + | chr7_3:63283638-63283657 | Msa1115760:CDS | 35.0% | |
| TAATTGTGGTTTCTTCGGAA+GGG | + | chr7_3:63283639-63283658 | Msa1115760:CDS | 35.0% | |
| TCAGTTGAAAACATCGTGAA+TGG | + | chr7_3:63283739-63283758 | Msa1115760:CDS | 35.0% | |
| ATCTCCATCTCATTAGAGAT+TGG | - | chr7_3:63283868-63283887 | None:intergenic | 35.0% | |
| CCAGCAGTTCTATAATCAAA+AGG | - | chr7_3:63284015-63284034 | None:intergenic | 35.0% | |
| !! | CCTTTTGATTATAGAACTGC+TGG | + | chr7_3:63284012-63284031 | Msa1115760:CDS | 35.0% |
| TGAATCCAACCCAGTAATTA+AGG | + | chr7_3:63284050-63284069 | Msa1115760:CDS | 35.0% | |
| GTTTATCCGCCTTAATTACT+GGG | - | chr7_3:63284062-63284081 | None:intergenic | 35.0% | |
| AGTTTATCCGCCTTAATTAC+TGG | - | chr7_3:63284063-63284082 | None:intergenic | 35.0% | |
| ATGGAGTCTCATGATGAGAT+GGG | + | chr7_3:63283580-63283599 | Msa1115760:CDS | 40.0% | |
| ACACTTGGAACACATGTTCA+TGG | - | chr7_3:63283671-63283690 | None:intergenic | 40.0% | |
| CATGTGTTCCAAGTGTTACA+AGG | + | chr7_3:63283675-63283694 | Msa1115760:CDS | 40.0% | |
| !!! | ATTGGATTTTCTGGAACCTC+GGG | - | chr7_3:63283850-63283869 | None:intergenic | 40.0% |
| !!! | GATTGGATTTTCTGGAACCT+CGG | - | chr7_3:63283851-63283870 | None:intergenic | 40.0% |
| CAGTTAATCCAACACGTTTC+CGG | - | chr7_3:63283927-63283946 | None:intergenic | 40.0% | |
| TTATAGAACTGCTGGTCAGA+AGG | + | chr7_3:63284020-63284039 | Msa1115760:CDS | 40.0% | |
| ATCCAACCCAGTAATTAAGG+CGG | + | chr7_3:63284053-63284072 | Msa1115760:CDS | 40.0% | |
| CAGTGTGTCCTTGTAACACT+TGG | - | chr7_3:63283686-63283705 | None:intergenic | 45.0% | |
| ATCGTGAATGGCAATTCCAG+CGG | + | chr7_3:63283751-63283770 | Msa1115760:CDS | 45.0% | |
| ! | TGATGTGCGAGTTGAAAGTG+TGG | + | chr7_3:63283807-63283826 | Msa1115760:CDS | 45.0% |
| ATGGAGATGAAAGCCAAGAC+TGG | + | chr7_3:63283880-63283899 | Msa1115760:CDS | 45.0% | |
| CGGAAACGTGTTGGATTAAC+TGG | + | chr7_3:63283925-63283944 | Msa1115760:CDS | 45.0% | |
| ! | ACTGGTTTCAGCTGCAAATG+TGG | + | chr7_3:63283943-63283962 | Msa1115760:CDS | 45.0% |
| AGCGATGCATTGAGCAAAAG+AGG | - | chr7_3:63283972-63283991 | None:intergenic | 45.0% | |
| ! | TACTGGGTTGGATTCAGCTA+TGG | - | chr7_3:63284046-63284065 | None:intergenic | 45.0% |
| ATCCGCCTTAATTACTGGGT+TGG | - | chr7_3:63284058-63284077 | None:intergenic | 45.0% | |
| GAATTGGACGTTCAGGTGTC+TGG | - | chr7_3:63283609-63283628 | None:intergenic | 50.0% | |
| ACGCACAGAATTGGACGTTC+AGG | - | chr7_3:63283616-63283635 | None:intergenic | 50.0% | |
| AATGGCAATTCCAGCGGCAA+TGG | + | chr7_3:63283757-63283776 | Msa1115760:CDS | 50.0% | |
| TCGCACATCAACAGTCCTTG+CGG | - | chr7_3:63283797-63283816 | None:intergenic | 50.0% | |
| ACTACTTGCCGGAAACGTGT+TGG | + | chr7_3:63283916-63283935 | Msa1115760:CDS | 50.0% | |
| CAAGGACACACTGCTGAAGC+AGG | + | chr7_3:63283693-63283712 | Msa1115760:CDS | 55.0% | |
| TTCCAGCGGCAATGGAAAGC+TGG | + | chr7_3:63283765-63283784 | Msa1115760:CDS | 55.0% | |
| GAAAGCTGGCTATGACCGCA+AGG | + | chr7_3:63283779-63283798 | Msa1115760:CDS | 55.0% | |
| GCTCAATACAGTGTCTCCCG+AGG | + | chr7_3:63283831-63283850 | Msa1115760:CDS | 55.0% | |
| CGGCAAGTAGTGCATCTGCT+GGG | - | chr7_3:63283907-63283926 | None:intergenic | 55.0% | |
| CCAGCAGATGCACTACTTGC+CGG | + | chr7_3:63283905-63283924 | Msa1115760:CDS | 55.0% | |
| CACACTGCTGAAGCAGGAGC+AGG | + | chr7_3:63283699-63283718 | Msa1115760:CDS | 60.0% | |
| AGCCAGCTTTCCATTGCCGC+TGG | - | chr7_3:63283770-63283789 | None:intergenic | 60.0% | |
| GCATCTGCTGGGACCAGTCT+TGG | - | chr7_3:63283896-63283915 | None:intergenic | 60.0% | |
| CCGGCAAGTAGTGCATCTGC+TGG | - | chr7_3:63283908-63283927 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 63283580 | 63284095 | 63283580 | ID=Msa1115760;Name=Msa1115760 |
| chr7_3 | mRNA | 63283580 | 63284095 | 63283580 | ID=Msa1115760-mRNA-1;Parent=Msa1115760;Name=Msa1115760-mRNA-1;_AED=0.37;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|171 |
| chr7_3 | exon | 63283580 | 63284095 | 63283580 | ID=Msa1115760-mRNA-1:exon:14797;Parent=Msa1115760-mRNA-1 |
| chr7_3 | CDS | 63283580 | 63284095 | 63283580 | ID=Msa1115760-mRNA-1:cds;Parent=Msa1115760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
