AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1128820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1128820 MtrunA17_Chr7g0273331 82.000 200 31 1 1 200 1 195 1.41e-112 319
Msa1128820 MtrunA17_Chr7g0273351 100.000 139 0 0 62 200 1 139 3.15e-96 276
Msa1128820 MtrunA17_Chr7g0268831 95.775 142 3 1 62 200 1 142 5.28e-92 265
Msa1128820 MtrunA17_Chr7g0259151 87.313 134 17 0 67 200 9 142 1.11e-80 236
Msa1128820 MtrunA17_Chr7g0273341 87.313 134 6 2 67 200 45 167 1.59e-73 219
Msa1128820 MtrunA17_Chr2g0319191 86.777 121 12 1 71 187 3 123 2.05e-68 205
Msa1128820 MtrunA17_Chr2g0319181 84.921 126 11 2 71 190 3 126 7.46e-68 203
Msa1128820 MtrunA17_Chr2g0327951 61.582 177 57 4 31 198 3 177 2.34e-59 184
Msa1128820 MtrunA17_Chr7g0272611 76.068 117 28 0 70 186 16 132 3.36e-58 179
Msa1128820 MtrunA17_Chr4g0031041 80.000 110 22 0 79 188 17 126 1.44e-57 178
Msa1128820 MtrunA17_Chr4g0031651 79.817 109 22 0 80 188 24 132 3.47e-57 177
Msa1128820 MtrunA17_Chr4g0031001 79.817 109 22 0 80 188 24 132 3.78e-57 177
Msa1128820 MtrunA17_Chr2g0327931 81.132 106 20 0 80 185 23 128 6.36e-57 176
Msa1128820 MtrunA17_Chr8g0379311 81.132 106 20 0 80 185 24 129 7.43e-57 176
Msa1128820 MtrunA17_Chr4g0035621 80.374 107 21 0 80 186 24 130 8.01e-57 176
Msa1128820 MtrunA17_Chr4g0032301 81.132 106 20 0 80 185 24 129 1.36e-56 176
Msa1128820 MtrunA17_Chr6g0449661 63.158 114 33 2 80 192 85 190 1.21e-40 137
Msa1128820 MtrunA17_Chr3g0119971 62.832 113 33 2 81 192 81 185 4.71e-40 135
Msa1128820 MtrunA17_Chr1g0148961 58.197 122 49 2 64 184 10 130 1.09e-38 129
Msa1128820 MtrunA17_Chr1g0148951 58.197 122 49 2 64 184 10 130 1.09e-38 129
Msa1128820 MtrunA17_Chr1g0211651 62.857 105 37 2 81 184 24 127 3.54e-37 125
Msa1128820 MtrunA17_Chr1g0184901 63.462 104 36 2 82 184 28 130 7.68e-37 125
Msa1128820 MtrunA17_Chr4g0031031 67.708 96 23 1 101 188 10 105 2.49e-35 120
Msa1128820 MtrunA17_Chr4g0024701 49.533 107 34 2 80 186 26 112 7.46e-25 94.0
Msa1128820 MtrunA17_Chr4g0000531 85.106 47 7 0 141 187 5 51 4.59e-21 82.4
Msa1128820 MtrunA17_Chr1g0183701 55.224 67 30 0 123 189 30 96 2.88e-18 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1128820 AT1G54690.1 93.478 138 9 0 63 200 5 142 3.34e-89 258
Msa1128820 AT1G08880.1 92.754 138 10 0 63 200 5 142 1.05e-88 257
Msa1128820 AT4G27230.2 86.290 124 13 1 71 190 3 126 5.61e-69 207
Msa1128820 AT4G27230.1 86.290 124 13 1 71 190 3 126 5.61e-69 207
Msa1128820 AT5G54640.1 82.946 129 19 1 62 190 1 126 1.29e-68 206
Msa1128820 AT1G51060.1 83.871 124 16 1 71 190 3 126 1.63e-67 203
Msa1128820 AT3G20670.1 84.677 124 15 1 71 190 3 126 6.40e-67 201
Msa1128820 AT5G59870.1 73.643 129 29 2 62 185 1 129 2.29e-59 183
Msa1128820 AT5G27670.1 73.438 128 29 1 63 185 3 130 7.98e-57 176
Msa1128820 AT5G02560.1 79.439 107 22 0 79 185 23 129 1.37e-55 174
Msa1128820 AT5G02560.2 64.885 131 22 1 79 185 23 153 2.77e-50 161
Msa1128820 AT2G38810.1 62.617 107 38 2 79 184 27 132 9.17e-38 127
Msa1128820 AT2G38810.4 62.617 107 38 2 79 184 27 132 9.17e-38 127
Msa1128820 AT2G38810.3 62.617 107 38 2 79 184 27 132 9.17e-38 127
Msa1128820 AT2G38810.2 62.617 107 38 2 79 184 27 132 9.17e-38 127
Msa1128820 AT1G52740.1 61.905 105 38 2 81 184 27 130 2.04e-37 127
Msa1128820 AT3G54560.1 60.952 105 39 2 81 184 29 132 4.09e-36 123
Msa1128820 AT3G54560.2 60.952 105 39 2 81 184 29 132 4.09e-36 123
Msa1128820 AT4G13570.1 43.220 118 65 2 62 178 2 118 3.22e-22 87.4
Msa1128820 AT4G13570.2 49.451 91 44 2 89 178 35 124 4.11e-21 84.7

Find 72 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAACCTAAAATTTGAATT+TGG 0.105689 7_3:-80703955 None:intergenic
TTGCCCTTTCCAACCTTCTT+AGG 0.222545 7_3:-80704463 None:intergenic
ATCTGCTTCTCAAGAATTCT+AGG 0.264115 7_3:+80704498 Msa1128820:CDS
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7_3:+80704384 Msa1128820:CDS
GAAACTGATTTGGCAGCCTT+TGG 0.309618 7_3:-80704039 None:intergenic
AAAACCTAAAATTTGAATTT+GGG 0.311933 7_3:-80703954 None:intergenic
ATTTGAATTTGGGAGAAATT+CGG 0.319539 7_3:-80703944 None:intergenic
AACACGTTCGGCGTATTTAC+CGG 0.321195 7_3:-80704121 None:intergenic
TGAAGATCTGGAAACTGATT+TGG 0.321558 7_3:-80704049 None:intergenic
GGAAAGGGCAAAGGAGAAAT+TGG 0.326882 7_3:+80704475 Msa1128820:CDS
TCTTAGCACTGAAGCTGAAT+TGG 0.340310 7_3:+80704689 Msa1128820:three_prime_UTR
AGCAAAGTCTGATGAATATT+AGG 0.347792 7_3:-80704439 None:intergenic
TTTACCGGCCTTGAGGAATC+TGG 0.369624 7_3:-80704106 None:intergenic
AAGTCCTGCCTTTGAAGATC+TGG 0.372304 7_3:-80704061 None:intergenic
TCAGACTTTGCTACCTAAGA+AGG 0.421667 7_3:+80704450 Msa1128820:CDS
GGCACATTCAGCTTGCTGTT+AGG 0.440630 7_3:+80704356 Msa1128820:CDS
GAGTTCCACTGGCACAACTA+AGG 0.456467 7_3:+80704002 Msa1128820:CDS
AGGACGGCGGAGAGGTAGAC+AGG 0.463287 7_3:-80704156 None:intergenic
GGGACTGTGACCATTGCTAA+TGG 0.468303 7_3:+80704406 Msa1128820:CDS
ACCCTATCCTCTGCGCTGAT+TGG 0.468439 7_3:-80703852 None:intergenic
ACCAATCAGCGCAGAGGATA+GGG 0.469649 7_3:+80703851 Msa1128820:CDS
AGCACCGGCACCAACACGTT+CGG 0.480590 7_3:-80704133 None:intergenic
CTGGCACAACTAAGGGCGGA+AGG 0.502885 7_3:+80704010 Msa1128820:CDS
GTTTCCAGATCTTCAAAGGC+AGG 0.505528 7_3:+80704057 Msa1128820:CDS
GCTAGTTGGCGTTGAACTGA+AGG 0.506003 7_3:+80704788 Msa1128820:three_prime_UTR
GAAAGCGAGAATAGATTTGA+GGG 0.507742 7_3:-80703904 None:intergenic
AATAGATTTGAGGGCTCAAT+CGG 0.508512 7_3:-80703895 None:intergenic
CTACCTAAGAAGGTTGGAAA+GGG 0.510822 7_3:+80704460 Msa1128820:CDS
GACCAATCAGCGCAGAGGAT+AGG 0.512883 7_3:+80703850 Msa1128820:CDS
TCTGCTTCTCAAGAATTCTA+GGG 0.519604 7_3:+80704499 Msa1128820:CDS
GGCACAACTAAGGGCGGAAG+GGG 0.525782 7_3:+80704012 Msa1128820:CDS
ATCAGTTTCCAGATCTTCAA+AGG 0.525986 7_3:+80704053 Msa1128820:CDS
AGAAAGCGAGAATAGATTTG+AGG 0.531168 7_3:-80703905 None:intergenic
AATTGGCTGGGAACGCTGCA+AGG 0.533557 7_3:+80704305 Msa1128820:CDS
ACTTTGCTACCTAAGAAGGT+TGG 0.535784 7_3:+80704454 Msa1128820:CDS
TGGCACAACTAAGGGCGGAA+GGG 0.543224 7_3:+80704011 Msa1128820:CDS
AGTTCCACTGGCACAACTAA+GGG 0.549244 7_3:+80704003 Msa1128820:CDS
TACGCCGAACGTGTTGGTGC+CGG 0.556200 7_3:+80704129 Msa1128820:CDS
GCTACCTAAGAAGGTTGGAA+AGG 0.557785 7_3:+80704459 Msa1128820:CDS
GCTGATTGGTCAATAGAGTA+AGG 0.560198 7_3:-80703838 None:intergenic
ATTGGCTGGGAACGCTGCAA+GGG 0.562499 7_3:+80704306 Msa1128820:CDS
ATCGCCAGATTCCTCAAGGC+CGG 0.565542 7_3:+80704102 Msa1128820:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.570928 7_3:-80704352 None:intergenic
GAATGTGCCTTGGTACAATA+CGG 0.571461 7_3:-80704342 None:intergenic
CTAGTGCTTAGTAAATTGAC+CGG 0.573561 7_3:+80704719 Msa1128820:three_prime_UTR
GGGCGGAAGGGGTAAGCCAA+AGG 0.573881 7_3:+80704023 Msa1128820:CDS
CGGCGTATTTACCGGCCTTG+AGG 0.577173 7_3:-80704113 None:intergenic
TCCGCCCTTAGTTGTGCCAG+TGG 0.580065 7_3:-80704007 None:intergenic
GAATCTGGCGATTCTTCCGA+CGG 0.590644 7_3:-80704091 None:intergenic
GGTAAATACGCCGAACGTGT+TGG 0.593895 7_3:+80704123 Msa1128820:CDS
AATCTGGCGATTCTTCCGAC+GGG 0.598431 7_3:-80704090 None:intergenic
CCTTGAGTATCTCGCTGCTG+AGG 0.612329 7_3:+80704176 Msa1128820:CDS
AAGAAGGTTGGAAAGGGCAA+AGG 0.621113 7_3:+80704466 Msa1128820:CDS
AGATTTGAGGGCTCAATCGG+TGG 0.626530 7_3:-80703892 None:intergenic
TAAAACACCACCATTAGCAA+TGG 0.630364 7_3:-80704416 None:intergenic
AAGAATCGCCAGATTCCTCA+AGG 0.631643 7_3:+80704098 Msa1128820:CDS
TCTGAGTGAGAATGCCACGC+TGG 0.633590 7_3:+80703806 Msa1128820:exon
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7_3:-80704176 None:intergenic
GGAGAGGTAGACAGGAGCAC+CGG 0.644805 7_3:-80704148 None:intergenic
TAAGCTATAATTGCTCAGAA+CGG 0.646047 7_3:-80704532 None:intergenic
TCCACTGGCACAACTAAGGG+CGG 0.647888 7_3:+80704006 Msa1128820:CDS
ACTGTGACCATTGCTAATGG+TGG 0.653058 7_3:+80704409 Msa1128820:CDS
AAAGTAATTAATTATCACAC+CGG 0.657466 7_3:-80704738 None:intergenic
GATACTCAAGGACGGCGGAG+AGG 0.669935 7_3:-80704164 None:intergenic
GCAGGACTTCAATTCCCCGT+CGG 0.683210 7_3:+80704075 Msa1128820:CDS
AGCAGCGAGATACTCAAGGA+CGG 0.684917 7_3:-80704172 None:intergenic
AGAAGAACCGTATTGTACCA+AGG 0.686303 7_3:+80704335 Msa1128820:CDS
TAAGGCACGGATCGCCAGCG+TGG 0.687680 7_3:-80703820 None:intergenic
TTGGTCAATAGAGTAAGGCA+CGG 0.694170 7_3:-80703833 None:intergenic
CTATTGACCAATCAGCGCAG+AGG 0.696432 7_3:+80703845 Msa1128820:CDS
ATCTGGCGATTCTTCCGACG+GGG 0.702228 7_3:-80704089 None:intergenic
AGCGAGATACTCAAGGACGG+CGG 0.736224 7_3:-80704169 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACCTAAAATTTGAATTT+GGG - chr7_3:80703957-80703976 None:intergenic 15.0%
!! AAAATACAGTTTAATCAAAT+TGG - chr7_3:80704263-80704282 None:intergenic 15.0%
!!! ATTAAACTGTATTTTCATTT+TGG + chr7_3:80704268-80704287 Msa1128820:intron 15.0%
!!! TTAATTACTTTTAGTTCATA+AGG + chr7_3:80704750-80704769 Msa1128820:three_prime_UTR 15.0%
!! AAAACCTAAAATTTGAATTT+GGG - chr7_3:80703957-80703976 None:intergenic 15.0%
!! AAAATACAGTTTAATCAAAT+TGG - chr7_3:80704263-80704282 None:intergenic 15.0%
!!! ATTAAACTGTATTTTCATTT+TGG + chr7_3:80704268-80704287 Msa1128820:intron 15.0%
!!! TTAATTACTTTTAGTTCATA+AGG + chr7_3:80704750-80704769 Msa1128820:three_prime_UTR 15.0%
!! GAAAACCTAAAATTTGAATT+TGG - chr7_3:80703958-80703977 None:intergenic 20.0%
!!! TCTCTTTTTTGTATAATGTA+GGG + chr7_3:80704229-80704248 Msa1128820:intron 20.0%
!!! GTATTTTCATTTTGGTATTT+AGG + chr7_3:80704276-80704295 Msa1128820:intron 20.0%
!!! AATACTATGCTTTTCATTAT+CGG - chr7_3:80704604-80704623 None:intergenic 20.0%
!! AAAGTAATTAATTATCACAC+CGG - chr7_3:80704741-80704760 None:intergenic 20.0%
!! GAAAACCTAAAATTTGAATT+TGG - chr7_3:80703958-80703977 None:intergenic 20.0%
!!! TCTCTTTTTTGTATAATGTA+GGG + chr7_3:80704229-80704248 Msa1128820:intron 20.0%
!!! GTATTTTCATTTTGGTATTT+AGG + chr7_3:80704276-80704295 Msa1128820:intron 20.0%
!!! AATACTATGCTTTTCATTAT+CGG - chr7_3:80704604-80704623 None:intergenic 20.0%
!! AAAGTAATTAATTATCACAC+CGG - chr7_3:80704741-80704760 None:intergenic 20.0%
! ATTTGAATTTGGGAGAAATT+CGG - chr7_3:80703947-80703966 None:intergenic 25.0%
!! TTCTCCCAAATTCAAATTTT+AGG + chr7_3:80703950-80703969 Msa1128820:CDS 25.0%
! ATACAAAAAAGAGAGTGAAA+GGG - chr7_3:80704223-80704242 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG - chr7_3:80704224-80704243 None:intergenic 25.0%
!!! CTCTCTTTTTTGTATAATGT+AGG + chr7_3:80704228-80704247 Msa1128820:intron 25.0%
!!! TCATTTTGGTATTTAGGTTT+TGG + chr7_3:80704282-80704301 Msa1128820:intron 25.0%
! ATTTGAATTTGGGAGAAATT+CGG - chr7_3:80703947-80703966 None:intergenic 25.0%
!! TTCTCCCAAATTCAAATTTT+AGG + chr7_3:80703950-80703969 Msa1128820:CDS 25.0%
! ATACAAAAAAGAGAGTGAAA+GGG - chr7_3:80704223-80704242 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG - chr7_3:80704224-80704243 None:intergenic 25.0%
!!! CTCTCTTTTTTGTATAATGT+AGG + chr7_3:80704228-80704247 Msa1128820:intron 25.0%
!!! TCATTTTGGTATTTAGGTTT+TGG + chr7_3:80704282-80704301 Msa1128820:intron 25.0%
!!! CGGTGGAATGTTTTTTATAT+AGG - chr7_3:80703878-80703897 None:intergenic 30.0%
!!! TGGTATTTAGGTTTTGGAAT+TGG + chr7_3:80704288-80704307 Msa1128820:intron 30.0%
AGCAAAGTCTGATGAATATT+AGG - chr7_3:80704442-80704461 None:intergenic 30.0%
TAAGCTATAATTGCTCAGAA+CGG - chr7_3:80704535-80704554 None:intergenic 30.0%
! AAAAAACTATGCTTGCTAGT+TGG + chr7_3:80704774-80704793 Msa1128820:three_prime_UTR 30.0%
!!! CGGTGGAATGTTTTTTATAT+AGG - chr7_3:80703878-80703897 None:intergenic 30.0%
!!! TGGTATTTAGGTTTTGGAAT+TGG + chr7_3:80704288-80704307 Msa1128820:intron 30.0%
AGCAAAGTCTGATGAATATT+AGG - chr7_3:80704442-80704461 None:intergenic 30.0%
TAAGCTATAATTGCTCAGAA+CGG - chr7_3:80704535-80704554 None:intergenic 30.0%
! AAAAAACTATGCTTGCTAGT+TGG + chr7_3:80704774-80704793 Msa1128820:three_prime_UTR 30.0%
AATAGATTTGAGGGCTCAAT+CGG - chr7_3:80703898-80703917 None:intergenic 35.0%
GAAAGCGAGAATAGATTTGA+GGG - chr7_3:80703907-80703926 None:intergenic 35.0%
AGAAAGCGAGAATAGATTTG+AGG - chr7_3:80703908-80703927 None:intergenic 35.0%
!! GTGGAACTCATTTTTCTGAT+CGG - chr7_3:80703991-80704010 None:intergenic 35.0%
ATCAGAAAAATGAGTTCCAC+TGG + chr7_3:80703991-80704010 Msa1128820:CDS 35.0%
TGAAGATCTGGAAACTGATT+TGG - chr7_3:80704052-80704071 None:intergenic 35.0%
ATCAGTTTCCAGATCTTCAA+AGG + chr7_3:80704053-80704072 Msa1128820:CDS 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG + chr7_3:80704292-80704311 Msa1128820:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG + chr7_3:80704293-80704312 Msa1128820:intron 35.0%
TAAAACACCACCATTAGCAA+TGG - chr7_3:80704419-80704438 None:intergenic 35.0%
ATCTGCTTCTCAAGAATTCT+AGG + chr7_3:80704498-80704517 Msa1128820:CDS 35.0%
TCTGCTTCTCAAGAATTCTA+GGG + chr7_3:80704499-80704518 Msa1128820:CDS 35.0%
! AGACTCTTCAGCATCATTTT+AGG - chr7_3:80704667-80704686 None:intergenic 35.0%
CTAGTGCTTAGTAAATTGAC+CGG + chr7_3:80704719-80704738 Msa1128820:three_prime_UTR 35.0%
AATAGATTTGAGGGCTCAAT+CGG - chr7_3:80703898-80703917 None:intergenic 35.0%
GAAAGCGAGAATAGATTTGA+GGG - chr7_3:80703907-80703926 None:intergenic 35.0%
AGAAAGCGAGAATAGATTTG+AGG - chr7_3:80703908-80703927 None:intergenic 35.0%
!! GTGGAACTCATTTTTCTGAT+CGG - chr7_3:80703991-80704010 None:intergenic 35.0%
ATCAGAAAAATGAGTTCCAC+TGG + chr7_3:80703991-80704010 Msa1128820:CDS 35.0%
TGAAGATCTGGAAACTGATT+TGG - chr7_3:80704052-80704071 None:intergenic 35.0%
ATCAGTTTCCAGATCTTCAA+AGG + chr7_3:80704053-80704072 Msa1128820:CDS 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG + chr7_3:80704292-80704311 Msa1128820:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG + chr7_3:80704293-80704312 Msa1128820:intron 35.0%
TAAAACACCACCATTAGCAA+TGG - chr7_3:80704419-80704438 None:intergenic 35.0%
ATCTGCTTCTCAAGAATTCT+AGG + chr7_3:80704498-80704517 Msa1128820:CDS 35.0%
TCTGCTTCTCAAGAATTCTA+GGG + chr7_3:80704499-80704518 Msa1128820:CDS 35.0%
! AGACTCTTCAGCATCATTTT+AGG - chr7_3:80704667-80704686 None:intergenic 35.0%
CTAGTGCTTAGTAAATTGAC+CGG + chr7_3:80704719-80704738 Msa1128820:three_prime_UTR 35.0%
TTGGTCAATAGAGTAAGGCA+CGG - chr7_3:80703836-80703855 None:intergenic 40.0%
! GCTGATTGGTCAATAGAGTA+AGG - chr7_3:80703841-80703860 None:intergenic 40.0%
GTGAAAGGGAACTTGAAGAA+AGG - chr7_3:80704209-80704228 None:intergenic 40.0%
AGAAGAACCGTATTGTACCA+AGG + chr7_3:80704335-80704354 Msa1128820:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG - chr7_3:80704345-80704364 None:intergenic 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7_3:80704384-80704403 Msa1128820:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7_3:80704385-80704404 Msa1128820:CDS 40.0%
! AAGAACTCAGCAAGCTTTTG+GGG + chr7_3:80704386-80704405 Msa1128820:CDS 40.0%
TCAGACTTTGCTACCTAAGA+AGG + chr7_3:80704450-80704469 Msa1128820:CDS 40.0%
ACTTTGCTACCTAAGAAGGT+TGG + chr7_3:80704454-80704473 Msa1128820:CDS 40.0%
CTACCTAAGAAGGTTGGAAA+GGG + chr7_3:80704460-80704479 Msa1128820:CDS 40.0%
! TCTTAGCACTGAAGCTGAAT+TGG + chr7_3:80704689-80704708 Msa1128820:three_prime_UTR 40.0%
TTGGTCAATAGAGTAAGGCA+CGG - chr7_3:80703836-80703855 None:intergenic 40.0%
! GCTGATTGGTCAATAGAGTA+AGG - chr7_3:80703841-80703860 None:intergenic 40.0%
GTGAAAGGGAACTTGAAGAA+AGG - chr7_3:80704209-80704228 None:intergenic 40.0%
AGAAGAACCGTATTGTACCA+AGG + chr7_3:80704335-80704354 Msa1128820:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG - chr7_3:80704345-80704364 None:intergenic 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7_3:80704384-80704403 Msa1128820:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7_3:80704385-80704404 Msa1128820:CDS 40.0%
! AAGAACTCAGCAAGCTTTTG+GGG + chr7_3:80704386-80704405 Msa1128820:CDS 40.0%
TCAGACTTTGCTACCTAAGA+AGG + chr7_3:80704450-80704469 Msa1128820:CDS 40.0%
ACTTTGCTACCTAAGAAGGT+TGG + chr7_3:80704454-80704473 Msa1128820:CDS 40.0%
CTACCTAAGAAGGTTGGAAA+GGG + chr7_3:80704460-80704479 Msa1128820:CDS 40.0%
! TCTTAGCACTGAAGCTGAAT+TGG + chr7_3:80704689-80704708 Msa1128820:three_prime_UTR 40.0%
AGTTCCACTGGCACAACTAA+GGG + chr7_3:80704003-80704022 Msa1128820:CDS 45.0%
!! GAAACTGATTTGGCAGCCTT+TGG - chr7_3:80704042-80704061 None:intergenic 45.0%
GTTTCCAGATCTTCAAAGGC+AGG + chr7_3:80704057-80704076 Msa1128820:CDS 45.0%
! AAGTCCTGCCTTTGAAGATC+TGG - chr7_3:80704064-80704083 None:intergenic 45.0%
AAGAATCGCCAGATTCCTCA+AGG + chr7_3:80704098-80704117 Msa1128820:CDS 45.0%
AACACGTTCGGCGTATTTAC+CGG - chr7_3:80704124-80704143 None:intergenic 45.0%
ACTGTGACCATTGCTAATGG+TGG + chr7_3:80704409-80704428 Msa1128820:CDS 45.0%
GCTACCTAAGAAGGTTGGAA+AGG + chr7_3:80704459-80704478 Msa1128820:CDS 45.0%
TTGCCCTTTCCAACCTTCTT+AGG - chr7_3:80704466-80704485 None:intergenic 45.0%
AAGAAGGTTGGAAAGGGCAA+AGG + chr7_3:80704466-80704485 Msa1128820:CDS 45.0%
GGAAAGGGCAAAGGAGAAAT+TGG + chr7_3:80704475-80704494 Msa1128820:CDS 45.0%
AGTTCCACTGGCACAACTAA+GGG + chr7_3:80704003-80704022 Msa1128820:CDS 45.0%
!! GAAACTGATTTGGCAGCCTT+TGG - chr7_3:80704042-80704061 None:intergenic 45.0%
GTTTCCAGATCTTCAAAGGC+AGG + chr7_3:80704057-80704076 Msa1128820:CDS 45.0%
! AAGTCCTGCCTTTGAAGATC+TGG - chr7_3:80704064-80704083 None:intergenic 45.0%
AAGAATCGCCAGATTCCTCA+AGG + chr7_3:80704098-80704117 Msa1128820:CDS 45.0%
AACACGTTCGGCGTATTTAC+CGG - chr7_3:80704124-80704143 None:intergenic 45.0%
ACTGTGACCATTGCTAATGG+TGG + chr7_3:80704409-80704428 Msa1128820:CDS 45.0%
GCTACCTAAGAAGGTTGGAA+AGG + chr7_3:80704459-80704478 Msa1128820:CDS 45.0%
TTGCCCTTTCCAACCTTCTT+AGG - chr7_3:80704466-80704485 None:intergenic 45.0%
AAGAAGGTTGGAAAGGGCAA+AGG + chr7_3:80704466-80704485 Msa1128820:CDS 45.0%
GGAAAGGGCAAAGGAGAAAT+TGG + chr7_3:80704475-80704494 Msa1128820:CDS 45.0%
CTATTGACCAATCAGCGCAG+AGG + chr7_3:80703845-80703864 Msa1128820:CDS 50.0%
ACCAATCAGCGCAGAGGATA+GGG + chr7_3:80703851-80703870 Msa1128820:CDS 50.0%
AGATTTGAGGGCTCAATCGG+TGG - chr7_3:80703895-80703914 None:intergenic 50.0%
GAGTTCCACTGGCACAACTA+AGG + chr7_3:80704002-80704021 Msa1128820:CDS 50.0%
AATCTGGCGATTCTTCCGAC+GGG - chr7_3:80704093-80704112 None:intergenic 50.0%
GAATCTGGCGATTCTTCCGA+CGG - chr7_3:80704094-80704113 None:intergenic 50.0%
TTTACCGGCCTTGAGGAATC+TGG - chr7_3:80704109-80704128 None:intergenic 50.0%
GGTAAATACGCCGAACGTGT+TGG + chr7_3:80704123-80704142 Msa1128820:CDS 50.0%
AGCAGCGAGATACTCAAGGA+CGG - chr7_3:80704175-80704194 None:intergenic 50.0%
ACAGCAAGCTGAATGTGCCT+TGG - chr7_3:80704355-80704374 None:intergenic 50.0%
!! GGCACATTCAGCTTGCTGTT+AGG + chr7_3:80704356-80704375 Msa1128820:CDS 50.0%
GGGACTGTGACCATTGCTAA+TGG + chr7_3:80704406-80704425 Msa1128820:CDS 50.0%
! GCTAGTTGGCGTTGAACTGA+AGG + chr7_3:80704788-80704807 Msa1128820:three_prime_UTR 50.0%
CTATTGACCAATCAGCGCAG+AGG + chr7_3:80703845-80703864 Msa1128820:CDS 50.0%
ACCAATCAGCGCAGAGGATA+GGG + chr7_3:80703851-80703870 Msa1128820:CDS 50.0%
AGATTTGAGGGCTCAATCGG+TGG - chr7_3:80703895-80703914 None:intergenic 50.0%
GAGTTCCACTGGCACAACTA+AGG + chr7_3:80704002-80704021 Msa1128820:CDS 50.0%
AATCTGGCGATTCTTCCGAC+GGG - chr7_3:80704093-80704112 None:intergenic 50.0%
GAATCTGGCGATTCTTCCGA+CGG - chr7_3:80704094-80704113 None:intergenic 50.0%
TTTACCGGCCTTGAGGAATC+TGG - chr7_3:80704109-80704128 None:intergenic 50.0%
GGTAAATACGCCGAACGTGT+TGG + chr7_3:80704123-80704142 Msa1128820:CDS 50.0%
AGCAGCGAGATACTCAAGGA+CGG - chr7_3:80704175-80704194 None:intergenic 50.0%
ACAGCAAGCTGAATGTGCCT+TGG - chr7_3:80704355-80704374 None:intergenic 50.0%
!! GGCACATTCAGCTTGCTGTT+AGG + chr7_3:80704356-80704375 Msa1128820:CDS 50.0%
GGGACTGTGACCATTGCTAA+TGG + chr7_3:80704406-80704425 Msa1128820:CDS 50.0%
! GCTAGTTGGCGTTGAACTGA+AGG + chr7_3:80704788-80704807 Msa1128820:three_prime_UTR 50.0%
TCTGAGTGAGAATGCCACGC+TGG + chr7_3:80703806-80703825 Msa1128820:exon 55.0%
GACCAATCAGCGCAGAGGAT+AGG + chr7_3:80703850-80703869 Msa1128820:CDS 55.0%
! ACCCTATCCTCTGCGCTGAT+TGG - chr7_3:80703855-80703874 None:intergenic 55.0%
TCCACTGGCACAACTAAGGG+CGG + chr7_3:80704006-80704025 Msa1128820:CDS 55.0%
TGGCACAACTAAGGGCGGAA+GGG + chr7_3:80704011-80704030 Msa1128820:CDS 55.0%
GCAGGACTTCAATTCCCCGT+CGG + chr7_3:80704075-80704094 Msa1128820:CDS 55.0%
ATCTGGCGATTCTTCCGACG+GGG - chr7_3:80704092-80704111 None:intergenic 55.0%
ATCGCCAGATTCCTCAAGGC+CGG + chr7_3:80704102-80704121 Msa1128820:CDS 55.0%
AGCGAGATACTCAAGGACGG+CGG - chr7_3:80704172-80704191 None:intergenic 55.0%
CCTCAGCAGCGAGATACTCA+AGG - chr7_3:80704179-80704198 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG + chr7_3:80704176-80704195 Msa1128820:CDS 55.0%
AATTGGCTGGGAACGCTGCA+AGG + chr7_3:80704305-80704324 Msa1128820:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG + chr7_3:80704306-80704325 Msa1128820:CDS 55.0%
TCTGAGTGAGAATGCCACGC+TGG + chr7_3:80703806-80703825 Msa1128820:exon 55.0%
GACCAATCAGCGCAGAGGAT+AGG + chr7_3:80703850-80703869 Msa1128820:CDS 55.0%
! ACCCTATCCTCTGCGCTGAT+TGG - chr7_3:80703855-80703874 None:intergenic 55.0%
TCCACTGGCACAACTAAGGG+CGG + chr7_3:80704006-80704025 Msa1128820:CDS 55.0%
TGGCACAACTAAGGGCGGAA+GGG + chr7_3:80704011-80704030 Msa1128820:CDS 55.0%
GCAGGACTTCAATTCCCCGT+CGG + chr7_3:80704075-80704094 Msa1128820:CDS 55.0%
ATCTGGCGATTCTTCCGACG+GGG - chr7_3:80704092-80704111 None:intergenic 55.0%
ATCGCCAGATTCCTCAAGGC+CGG + chr7_3:80704102-80704121 Msa1128820:CDS 55.0%
AGCGAGATACTCAAGGACGG+CGG - chr7_3:80704172-80704191 None:intergenic 55.0%
CCTCAGCAGCGAGATACTCA+AGG - chr7_3:80704179-80704198 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG + chr7_3:80704176-80704195 Msa1128820:CDS 55.0%
AATTGGCTGGGAACGCTGCA+AGG + chr7_3:80704305-80704324 Msa1128820:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG + chr7_3:80704306-80704325 Msa1128820:CDS 55.0%
TCCGCCCTTAGTTGTGCCAG+TGG - chr7_3:80704010-80704029 None:intergenic 60.0%
CTGGCACAACTAAGGGCGGA+AGG + chr7_3:80704010-80704029 Msa1128820:CDS 60.0%
GGCACAACTAAGGGCGGAAG+GGG + chr7_3:80704012-80704031 Msa1128820:CDS 60.0%
CGGCGTATTTACCGGCCTTG+AGG - chr7_3:80704116-80704135 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG + chr7_3:80704129-80704148 Msa1128820:CDS 60.0%
!! AGCACCGGCACCAACACGTT+CGG - chr7_3:80704136-80704155 None:intergenic 60.0%
! GGAGAGGTAGACAGGAGCAC+CGG - chr7_3:80704151-80704170 None:intergenic 60.0%
GATACTCAAGGACGGCGGAG+AGG - chr7_3:80704167-80704186 None:intergenic 60.0%
TCCGCCCTTAGTTGTGCCAG+TGG - chr7_3:80704010-80704029 None:intergenic 60.0%
CTGGCACAACTAAGGGCGGA+AGG + chr7_3:80704010-80704029 Msa1128820:CDS 60.0%
GGCACAACTAAGGGCGGAAG+GGG + chr7_3:80704012-80704031 Msa1128820:CDS 60.0%
CGGCGTATTTACCGGCCTTG+AGG - chr7_3:80704116-80704135 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG + chr7_3:80704129-80704148 Msa1128820:CDS 60.0%
!! AGCACCGGCACCAACACGTT+CGG - chr7_3:80704136-80704155 None:intergenic 60.0%
! GGAGAGGTAGACAGGAGCAC+CGG - chr7_3:80704151-80704170 None:intergenic 60.0%
GATACTCAAGGACGGCGGAG+AGG - chr7_3:80704167-80704186 None:intergenic 60.0%
TAAGGCACGGATCGCCAGCG+TGG - chr7_3:80703823-80703842 None:intergenic 65.0%
GGGCGGAAGGGGTAAGCCAA+AGG + chr7_3:80704023-80704042 Msa1128820:CDS 65.0%
AGGACGGCGGAGAGGTAGAC+AGG - chr7_3:80704159-80704178 None:intergenic 65.0%
TAAGGCACGGATCGCCAGCG+TGG - chr7_3:80703823-80703842 None:intergenic 65.0%
GGGCGGAAGGGGTAAGCCAA+AGG + chr7_3:80704023-80704042 Msa1128820:CDS 65.0%
AGGACGGCGGAGAGGTAGAC+AGG - chr7_3:80704159-80704178 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 80703796 80704813 80703796 ID=Msa1128820;Name=Msa1128820
chr7_3 mRNA 80703796 80704813 80703796 ID=Msa1128820-mRNA-1;Parent=Msa1128820;Name=Msa1128820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=21|1|1|1|1|1|2|294|200
chr7_3 exon 80703796 80704197 80703796 ID=Msa1128820-mRNA-1:exon:23240;Parent=Msa1128820-mRNA-1
chr7_3 exon 80704298 80704813 80704298 ID=Msa1128820-mRNA-1:exon:23241;Parent=Msa1128820-mRNA-1
chr7_3 five_prime_UTR 80703796 80703816 80703796 ID=Msa1128820-mRNA-1:five_prime_utr;Parent=Msa1128820-mRNA-1
chr7_3 CDS 80703817 80704197 80703817 ID=Msa1128820-mRNA-1:cds;Parent=Msa1128820-mRNA-1
chr7_3 CDS 80704298 80704519 80704298 ID=Msa1128820-mRNA-1:cds;Parent=Msa1128820-mRNA-1
chr7_3 three_prime_UTR 80704520 80704813 80704520 ID=Msa1128820-mRNA-1:three_prime_utr;Parent=Msa1128820-mRNA-1
Gene Sequence

>Msa1128820

ATGCCACGCTGGCGATCCGTGCCTTACTCTATTGACCAATCAGCGCAGAGGATAGGGTCCTATATAAAAAACATTCCACCGATTGAGCCCTCAAATCTATTCTCGCTTTCTCTCACTCTTCTCAAATCCGAATTTCTCCCAAATTCAAATTTTAGGTTTTCGATTTCTCATCCGATCAGAAAAATGAGTTCCACTGGCACAACTAAGGGCGGAAGGGGTAAGCCAAAGGCTGCCAAATCAGTTTCCAGATCTTCAAAGGCAGGACTTCAATTCCCCGTCGGAAGAATCGCCAGATTCCTCAAGGCCGGTAAATACGCCGAACGTGTTGGTGCCGGTGCTCCTGTCTACCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTTTTGGAATTGGCTGGGAACGCTGCAAGGGACAACAAGAAGAACCGTATTGTACCAAGGCACATTCAGCTTGCTGTTAGGAACGATGAAGAACTCAGCAAGCTTTTGGGGACTGTGACCATTGCTAATGGTGGTGTTTTACCTAATATTCATCAGACTTTGCTACCTAAGAAGGTTGGAAAGGGCAAAGGAGAAATTGGATCTGCTTCTCAAGAATTCTAG
Protein sequence

>Msa1128820

MPRWRSVPYSIDQSAQRIGSYIKNIPPIEPSNLFSLSLTLLKSEFLPNSNFRFSISHPIRKMSSTGTTKGGRGKPKAAKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF*