AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1424370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1424370 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa1424370 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1424370 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1424370 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1424370 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa1424370 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa1424370 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa1424370 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa1424370 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa1424370 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa1424370 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa1424370 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa1424370 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa1424370 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa1424370 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa1424370 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1424370 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa1424370 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa1424370 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa1424370 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa1424370 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa1424370 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa1424370 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa1424370 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa1424370 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa1424370 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa1424370 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa1424370 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa1424370 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa1424370 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa1424370 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa1424370 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 39 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTCTCTAACCAATCTT+TGG 0.224743 tig0025690:-13805 None:intergenic
GCCGCTGAAGCTTATCTTGT+TGG 0.348800 tig0025690:+13887 Msa1424370:CDS
TTGAATATCCTTAGGCATAA+TGG 0.349539 tig0025690:-13957 None:intergenic
AGCTGATTTCCGAGCGGCTT+TGG 0.355177 tig0025690:-13669 None:intergenic
TTCTTCACTCCTCCGGTGGC+TGG 0.371943 tig0025690:-13692 None:intergenic
TCACGAAGAGCAACAGTTCC+AGG 0.402245 tig0025690:-13731 None:intergenic
GAAGCTTCCATTCCAAAGAT+TGG 0.412914 tig0025690:+13793 Msa1424370:CDS
CTTGCAAGTTGAATATCCTT+AGG 0.413826 tig0025690:-13965 None:intergenic
AGGATTTCAAAACTGATCTC+AGG 0.429009 tig0025690:+13831 Msa1424370:CDS
GGATTTGCGAGCGGTTTGTT+TGG 0.441284 tig0025690:-13612 None:intergenic
AGAGCACTGAGCTTCTCATC+AGG 0.458709 tig0025690:+13771 Msa1424370:CDS
TTGGTGGCGAGTTGTTTCCT+TGG 0.473034 tig0025690:-13650 None:intergenic
GGCTTCTTGAAGAGCAGAAA+CGG 0.474881 tig0025690:-13867 None:intergenic
TTTCCTTGGAGCTTTGCCTC+CGG 0.476973 tig0025690:-13636 None:intergenic
CCTTGGAGCTTTGCCTCCGG+TGG 0.489182 tig0025690:-13633 None:intergenic
TGATTTCCGAGCGGCTTTGG+TGG 0.495094 tig0025690:-13666 None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG 0.509508 tig0025690:-13699 None:intergenic
TCTCTAACCAATCTTTGGAA+TGG 0.520211 tig0025690:-13800 None:intergenic
CGGAAATCAGCTCCAGCCAC+CGG 0.530295 tig0025690:+13680 Msa1424370:CDS
GAGAGTTACCATTATGCCTA+AGG 0.535535 tig0025690:+13949 Msa1424370:CDS
AGCATGAATAGCACAAAGGT+TGG 0.570443 tig0025690:-13924 None:intergenic
AACTCGCCACCAAAGCCGCT+CGG 0.570459 tig0025690:+13660 Msa1424370:CDS
CCACCGGAGGCAAAGCTCCA+AGG 0.572363 tig0025690:+13633 Msa1424370:CDS
CAGTGCTCTTCTGATACTTG+CGG 0.590815 tig0025690:-13757 None:intergenic
AAGAAGAATCAGAGGCGAGA+GGG 0.597241 tig0025690:+13985 Msa1424370:CDS
ATTGGTTAGAGAAATCGCTC+AGG 0.600201 tig0025690:+13811 Msa1424370:CDS
AAGCCACACAGATTCCGTCC+TGG 0.622196 tig0025690:+13713 Msa1424370:CDS
CAAACCGCTCGCAAATCCAC+CGG 0.629023 tig0025690:+13617 Msa1424370:CDS
TGGCTTCTTCACTCCTCCGG+TGG 0.631443 tig0025690:-13696 None:intergenic
AAATCAGCTCCAGCCACCGG+AGG 0.637716 tig0025690:+13683 Msa1424370:CDS
GCCTCCGGTGGATTTGCGAG+CGG 0.639769 tig0025690:-13621 None:intergenic
ACCGCTCGCAAATCCACCGG+AGG 0.648108 tig0025690:+13620 Msa1424370:CDS
CAAGAAGAATCAGAGGCGAG+AGG 0.648850 tig0025690:+13984 Msa1424370:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.660238 tig0025690:+13977 Msa1424370:CDS
ACCAACAAGATAAGCTTCAG+CGG 0.667251 tig0025690:-13888 None:intergenic
GAAGAGCAACAGTTCCAGGA+CGG 0.681197 tig0025690:-13727 None:intergenic
TCTTAGCATGAATAGCACAA+AGG 0.697580 tig0025690:-13928 None:intergenic
GTTCCAGGACGGAATCTGTG+TGG 0.699556 tig0025690:-13716 None:intergenic
GGCTGGAGCTGATTTCCGAG+CGG 0.733993 tig0025690:-13675 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGAATATCCTTAGGCATAA+TGG - tig0025690:13960-13979 None:intergenic 30.0%
! TCTCTAACCAATCTTTGGAA+TGG - tig0025690:13803-13822 None:intergenic 35.0%
CGATTTCTCTAACCAATCTT+TGG - tig0025690:13808-13827 None:intergenic 35.0%
AGGATTTCAAAACTGATCTC+AGG + tig0025690:13831-13850 Msa1424370:CDS 35.0%
TCTTAGCATGAATAGCACAA+AGG - tig0025690:13931-13950 None:intergenic 35.0%
! CTTGCAAGTTGAATATCCTT+AGG - tig0025690:13968-13987 None:intergenic 35.0%
GAAGCTTCCATTCCAAAGAT+TGG + tig0025690:13793-13812 Msa1424370:CDS 40.0%
ATTGGTTAGAGAAATCGCTC+AGG + tig0025690:13811-13830 Msa1424370:CDS 40.0%
ACCAACAAGATAAGCTTCAG+CGG - tig0025690:13891-13910 None:intergenic 40.0%
AGCATGAATAGCACAAAGGT+TGG - tig0025690:13927-13946 None:intergenic 40.0%
GAGAGTTACCATTATGCCTA+AGG + tig0025690:13949-13968 Msa1424370:CDS 40.0%
CAACTTGCAAGAAGAATCAG+AGG + tig0025690:13977-13996 Msa1424370:CDS 40.0%
CAGTGCTCTTCTGATACTTG+CGG - tig0025690:13760-13779 None:intergenic 45.0%
GGCTTCTTGAAGAGCAGAAA+CGG - tig0025690:13870-13889 None:intergenic 45.0%
AAGAAGAATCAGAGGCGAGA+GGG + tig0025690:13985-14004 Msa1424370:CDS 45.0%
! GGATTTGCGAGCGGTTTGTT+TGG - tig0025690:13615-13634 None:intergenic 50.0%
TTTCCTTGGAGCTTTGCCTC+CGG - tig0025690:13639-13658 None:intergenic 50.0%
!! TTGGTGGCGAGTTGTTTCCT+TGG - tig0025690:13653-13672 None:intergenic 50.0%
GAAGAGCAACAGTTCCAGGA+CGG - tig0025690:13730-13749 None:intergenic 50.0%
TCACGAAGAGCAACAGTTCC+AGG - tig0025690:13734-13753 None:intergenic 50.0%
!! AGAGCACTGAGCTTCTCATC+AGG + tig0025690:13771-13790 Msa1424370:CDS 50.0%
GCCGCTGAAGCTTATCTTGT+TGG + tig0025690:13887-13906 Msa1424370:CDS 50.0%
CAAGAAGAATCAGAGGCGAG+AGG + tig0025690:13984-14003 Msa1424370:CDS 50.0%
CAAACCGCTCGCAAATCCAC+CGG + tig0025690:13617-13636 Msa1424370:CDS 55.0%
! TGATTTCCGAGCGGCTTTGG+TGG - tig0025690:13669-13688 None:intergenic 55.0%
! AGCTGATTTCCGAGCGGCTT+TGG - tig0025690:13672-13691 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG - tig0025690:13702-13721 None:intergenic 55.0%
AAGCCACACAGATTCCGTCC+TGG + tig0025690:13713-13732 Msa1424370:CDS 55.0%
GTTCCAGGACGGAATCTGTG+TGG - tig0025690:13719-13738 None:intergenic 55.0%
AACTCGCCACCAAAGCCGCT+CGG + tig0025690:13660-13679 Msa1424370:CDS 60.0%
! GGCTGGAGCTGATTTCCGAG+CGG - tig0025690:13678-13697 None:intergenic 60.0%
CGGAAATCAGCTCCAGCCAC+CGG + tig0025690:13680-13699 Msa1424370:CDS 60.0%
AAATCAGCTCCAGCCACCGG+AGG + tig0025690:13683-13702 Msa1424370:CDS 60.0%
TTCTTCACTCCTCCGGTGGC+TGG - tig0025690:13695-13714 None:intergenic 60.0%
TGGCTTCTTCACTCCTCCGG+TGG - tig0025690:13699-13718 None:intergenic 60.0%
ACCGCTCGCAAATCCACCGG+AGG + tig0025690:13620-13639 Msa1424370:CDS 65.0%
! GCCTCCGGTGGATTTGCGAG+CGG - tig0025690:13624-13643 None:intergenic 65.0%
CCTTGGAGCTTTGCCTCCGG+TGG - tig0025690:13636-13655 None:intergenic 65.0%
CCACCGGAGGCAAAGCTCCA+AGG + tig0025690:13633-13652 Msa1424370:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0025690 gene 13602 14012 13602 ID=Msa1424370;Name=Msa1424370
tig0025690 mRNA 13602 14012 13602 ID=Msa1424370-mRNA-1;Parent=Msa1424370;Name=Msa1424370-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|136
tig0025690 exon 13602 14012 13602 ID=Msa1424370-mRNA-1:exon:3743;Parent=Msa1424370-mRNA-1
tig0025690 CDS 13602 14012 13602 ID=Msa1424370-mRNA-1:cds;Parent=Msa1424370-mRNA-1
Gene Sequence

>Msa1424370

ATGGCTCGTACCAAACAAACCGCTCGCAAATCCACCGGAGGCAAAGCTCCAAGGAAACAACTCGCCACCAAAGCCGCTCGGAAATCAGCTCCAGCCACCGGAGGAGTGAAGAAGCCACACAGATTCCGTCCTGGAACTGTTGCTCTTCGTGAGATCCGCAAGTATCAGAAGAGCACTGAGCTTCTCATCAGGAAGCTTCCATTCCAAAGATTGGTTAGAGAAATCGCTCAGGATTTCAAAACTGATCTCAGGTTTCAGAGTAGCGCCGTTTCTGCTCTTCAAGAAGCCGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACCAACCTTTGTGCTATTCATGCTAAGAGAGTTACCATTATGCCTAAGGATATTCAACTTGCAAGAAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>Msa1424370

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*