AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1410190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410190 MtrunA17_Chr7g0268831 100.000 142 0 0 1 142 1 142 1.91e-99 281
Msa1410190 MtrunA17_Chr7g0273351 95.775 142 3 1 1 142 1 139 3.56e-93 265
Msa1410190 MtrunA17_Chr7g0273331 95.775 142 3 1 1 142 57 195 2.66e-92 265
Msa1410190 MtrunA17_Chr7g0259151 84.507 142 22 0 1 142 1 142 1.23e-84 244
Msa1410190 MtrunA17_Chr7g0273341 87.407 135 6 2 8 142 44 167 8.50e-76 223
Msa1410190 MtrunA17_Chr2g0319191 86.777 121 12 1 13 129 3 123 2.61e-69 205
Msa1410190 MtrunA17_Chr2g0319181 85.484 124 14 1 13 132 3 126 2.73e-69 204
Msa1410190 MtrunA17_Chr2g0327951 75.000 120 30 0 21 140 58 177 6.88e-60 183
Msa1410190 MtrunA17_Chr7g0272611 76.068 117 28 0 12 128 16 132 9.95e-60 181
Msa1410190 MtrunA17_Chr4g0031041 80.000 110 22 0 21 130 17 126 2.97e-59 180
Msa1410190 MtrunA17_Chr4g0031001 79.817 109 22 0 22 130 24 132 8.20e-59 179
Msa1410190 MtrunA17_Chr4g0031651 79.817 109 22 0 22 130 24 132 8.57e-59 179
Msa1410190 MtrunA17_Chr4g0035621 80.374 107 21 0 22 128 24 130 1.86e-58 178
Msa1410190 MtrunA17_Chr2g0327931 81.132 106 20 0 22 127 23 128 1.88e-58 178
Msa1410190 MtrunA17_Chr8g0379311 81.132 106 20 0 22 127 24 129 2.47e-58 177
Msa1410190 MtrunA17_Chr4g0032301 81.132 106 20 0 22 127 24 129 3.60e-58 177
Msa1410190 MtrunA17_Chr6g0449661 63.158 114 33 2 22 134 85 190 3.82e-42 138
Msa1410190 MtrunA17_Chr3g0119971 62.832 113 33 2 23 134 81 185 1.20e-41 137
Msa1410190 MtrunA17_Chr1g0148961 58.333 132 47 4 1 126 1 130 2.61e-40 131
Msa1410190 MtrunA17_Chr1g0148951 58.333 132 47 4 1 126 1 130 2.61e-40 131
Msa1410190 MtrunA17_Chr1g0211651 56.250 128 53 3 1 126 1 127 2.03e-39 129
Msa1410190 MtrunA17_Chr1g0184901 63.462 104 36 2 24 126 28 130 3.79e-38 126
Msa1410190 MtrunA17_Chr4g0031031 67.708 96 23 1 43 130 10 105 2.85e-36 120
Msa1410190 MtrunA17_Chr4g0024701 49.533 107 34 2 22 128 26 112 3.37e-26 95.1
Msa1410190 MtrunA17_Chr4g0000531 85.106 47 7 0 83 129 5 51 2.14e-22 84.0
Msa1410190 MtrunA17_Chr1g0183701 55.224 67 30 0 65 131 30 96 3.59e-19 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410190 AT1G54690.1 92.254 142 11 0 1 142 1 142 3.22e-91 261
Msa1410190 AT1G08880.1 90.845 142 13 0 1 142 1 142 2.40e-90 259
Msa1410190 AT4G27230.2 86.290 124 13 1 13 132 3 126 4.59e-70 207
Msa1410190 AT4G27230.1 86.290 124 13 1 13 132 3 126 4.59e-70 207
Msa1410190 AT5G54640.1 80.303 132 20 1 1 132 1 126 3.20e-69 205
Msa1410190 AT1G51060.1 83.871 124 16 1 13 132 3 126 1.55e-68 203
Msa1410190 AT3G20670.1 84.677 124 15 1 13 132 3 126 4.15e-68 202
Msa1410190 AT5G59870.1 72.093 129 34 1 1 127 1 129 7.35e-61 184
Msa1410190 AT5G27670.1 72.656 128 33 1 2 127 3 130 2.23e-58 178
Msa1410190 AT5G02560.1 79.439 107 22 0 21 127 23 129 1.76e-57 176
Msa1410190 AT5G02560.2 64.885 131 22 1 21 127 23 153 4.90e-52 163
Msa1410190 AT2G38810.1 62.617 107 38 2 21 126 27 132 6.26e-39 128
Msa1410190 AT2G38810.4 62.617 107 38 2 21 126 27 132 6.26e-39 128
Msa1410190 AT2G38810.3 62.617 107 38 2 21 126 27 132 6.26e-39 128
Msa1410190 AT2G38810.2 62.617 107 38 2 21 126 27 132 6.26e-39 128
Msa1410190 AT1G52740.1 55.224 134 48 4 1 126 1 130 7.02e-39 128
Msa1410190 AT3G54560.1 60.952 105 39 2 23 126 29 132 2.08e-37 124
Msa1410190 AT3G54560.2 60.952 105 39 2 23 126 29 132 2.08e-37 124
Msa1410190 AT4G13570.1 44.348 115 62 2 7 120 5 118 4.26e-23 87.8
Msa1410190 AT4G13570.2 49.451 91 44 2 31 120 35 124 3.55e-22 85.5

Find 42 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCTCGCTGCTGAGGTTT+TGG 0.254221 tig0023619:-45184 Msa1410190:CDS
TGAAGAACTCAGCAAGCTTT+TGG 0.258146 tig0023619:-45082 Msa1410190:CDS
AAGCAATGTCTGATGAATAT+TGG 0.288668 tig0023619:+45026 None:intergenic
GAAACGGATTTGGAGGCTTT+TGG 0.298714 tig0023619:+45327 None:intergenic
TGAAGATCTTGAAACGGATT+TGG 0.320293 tig0023619:+45317 None:intergenic
TGGTTTGCCTCTTCCTCCTT+TGG 0.327874 tig0023619:+45347 None:intergenic
GGAAAAGGGAAAGGTGAAAT+TGG 0.334923 tig0023619:-44991 Msa1410190:CDS
GGTTGTAGTTGTGGCACCTT+TGG 0.379726 tig0023619:+45368 None:intergenic
TCAGACATTGCTTCCTAAGA+AGG 0.403914 tig0023619:-45016 Msa1410190:CDS
AGCAATGTCTGATGAATATT+GGG 0.409255 tig0023619:+45027 None:intergenic
TCCTCCTTTGGTTGTAGTTG+TGG 0.413422 tig0023619:+45359 None:intergenic
CTTCCTAAGAAGGTTGGAAA+AGG 0.418428 tig0023619:-45006 Msa1410190:CDS
GCGTGTTGGCGCCGGTGCTC+CGG 0.455502 tig0023619:-45229 Msa1410190:CDS
TACGCCGAGCGTGTTGGCGC+CGG 0.470776 tig0023619:-45237 Msa1410190:CDS
AGATCTTGAAACGGATTTGG+AGG 0.479759 tig0023619:+45320 None:intergenic
GGTGCTGTAACCATTGCTAA+TGG 0.480768 tig0023619:-45060 Msa1410190:CDS
AGCACCGGCGCCAACACGCT+CGG 0.487757 tig0023619:+45233 None:intergenic
ATCGCAAGATTCCTCAAGGC+TGG 0.491107 tig0023619:-45264 Msa1410190:CDS
ACATTGCTTCCTAAGAAGGT+TGG 0.502061 tig0023619:-45012 Msa1410190:CDS
GGAAAATACGCCGAGCGTGT+TGG 0.518396 tig0023619:-45243 Msa1410190:CDS
TTCCTAAGAAGGTTGGAAAA+GGG 0.529191 tig0023619:-45005 Msa1410190:CDS
GTTTCAAGATCTTCAAAAGC+CGG 0.540874 tig0023619:-45309 Msa1410190:CDS
TCCGACGGGGAATTGAAGAC+CGG 0.548728 tig0023619:+45290 None:intergenic
AGAGAGATAGACCGGAGCAC+CGG 0.550862 tig0023619:+45218 None:intergenic
AGAACAGCAGAGAGATAGAC+CGG 0.553809 tig0023619:+45210 None:intergenic
AATCTTGCGATTCTTCCGAC+GGG 0.559881 tig0023619:+45276 None:intergenic
GAATCTTGCGATTCTTCCGA+CGG 0.569168 tig0023619:+45275 None:intergenic
ACTACAACCAAAGGAGGAAG+AGG 0.569188 tig0023619:-45354 Msa1410190:CDS
ACAGCAAGCTGAATGTGTCT+CGG 0.587168 tig0023619:+45114 None:intergenic
GCTGTAACCATTGCTAATGG+TGG 0.619444 tig0023619:-45057 Msa1410190:CDS
AAGAAGGTTGGAAAAGGGAA+AGG 0.622701 tig0023619:-45000 Msa1410190:CDS
AATCCGAAAATGAGTTCCAA+AGG 0.625540 tig0023619:-45384 None:intergenic
AAGAATCGCAAGATTCCTCA+AGG 0.631895 tig0023619:-45268 Msa1410190:CDS
GGTGCCACAACTACAACCAA+AGG 0.634663 tig0023619:-45363 Msa1410190:CDS
CAAAACACCACCATTAGCAA+TGG 0.637204 tig0023619:+45050 None:intergenic
TCTCGAGTATCTCGCTGCTG+AGG 0.640871 tig0023619:-45190 Msa1410190:CDS
GCCGGTCTTCAATTCCCCGT+CGG 0.642556 tig0023619:-45291 Msa1410190:CDS
GCCACAACTACAACCAAAGG+AGG 0.646292 tig0023619:-45360 Msa1410190:CDS
ATTGGCTGGTAATGCTGCGA+GGG 0.649014 tig0023619:-45160 Msa1410190:CDS
GACACATTCAGCTTGCTGTG+AGG 0.685856 tig0023619:-45110 Msa1410190:CDS
ATCTTGCGATTCTTCCGACG+GGG 0.695192 tig0023619:+45277 None:intergenic
AATTGGCTGGTAATGCTGCG+AGG 0.697250 tig0023619:-45161 Msa1410190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGCAATGTCTGATGAATATT+GGG + tig0023619:45320-45339 None:intergenic 30.0%
AAGCAATGTCTGATGAATAT+TGG + tig0023619:45321-45340 None:intergenic 30.0%
! TGAAGATCTTGAAACGGATT+TGG + tig0023619:45030-45049 None:intergenic 35.0%
!! GGCTTTTGAAGATCTTGAAA+CGG + tig0023619:45036-45055 None:intergenic 35.0%
GTTTCAAGATCTTCAAAAGC+CGG - tig0023619:45035-45054 Msa1410190:CDS 35.0%
TTCCTAAGAAGGTTGGAAAA+GGG - tig0023619:45339-45358 Msa1410190:CDS 35.0%
! AGATCTTGAAACGGATTTGG+AGG + tig0023619:45027-45046 None:intergenic 40.0%
AAGAATCGCAAGATTCCTCA+AGG - tig0023619:45076-45095 Msa1410190:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG - tig0023619:45262-45281 Msa1410190:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG - tig0023619:45263-45282 Msa1410190:CDS 40.0%
CAAAACACCACCATTAGCAA+TGG + tig0023619:45297-45316 None:intergenic 40.0%
TCAGACATTGCTTCCTAAGA+AGG - tig0023619:45328-45347 Msa1410190:CDS 40.0%
ACATTGCTTCCTAAGAAGGT+TGG - tig0023619:45332-45351 Msa1410190:CDS 40.0%
CTTCCTAAGAAGGTTGGAAA+AGG - tig0023619:45338-45357 Msa1410190:CDS 40.0%
! TTCCCTTTTCCAACCTTCTT+AGG + tig0023619:45344-45363 None:intergenic 40.0%
AAGAAGGTTGGAAAAGGGAA+AGG - tig0023619:45344-45363 Msa1410190:CDS 40.0%
GGAAAAGGGAAAGGTGAAAT+TGG - tig0023619:45353-45372 Msa1410190:CDS 40.0%
TCCTCCTTTGGTTGTAGTTG+TGG + tig0023619:44988-45007 None:intergenic 45.0%
ACTACAACCAAAGGAGGAAG+AGG - tig0023619:44990-45009 Msa1410190:CDS 45.0%
!! GAAACGGATTTGGAGGCTTT+TGG + tig0023619:45020-45039 None:intergenic 45.0%
AATCTTGCGATTCTTCCGAC+GGG + tig0023619:45071-45090 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG + tig0023619:45072-45091 None:intergenic 45.0%
AGAACAGCAGAGAGATAGAC+CGG + tig0023619:45137-45156 None:intergenic 45.0%
ACAGCAAGCTGAATGTGTCT+CGG + tig0023619:45233-45252 None:intergenic 45.0%
! GGTGCTGTAACCATTGCTAA+TGG - tig0023619:45284-45303 Msa1410190:CDS 45.0%
GCTGTAACCATTGCTAATGG+TGG - tig0023619:45287-45306 Msa1410190:CDS 45.0%
GGTTGTAGTTGTGGCACCTT+TGG + tig0023619:44979-44998 None:intergenic 50.0%
! GGTGCCACAACTACAACCAA+AGG - tig0023619:44981-45000 Msa1410190:CDS 50.0%
GCCACAACTACAACCAAAGG+AGG - tig0023619:44984-45003 Msa1410190:CDS 50.0%
! TGGTTTGCCTCTTCCTCCTT+TGG + tig0023619:45000-45019 None:intergenic 50.0%
ATCTTGCGATTCTTCCGACG+GGG + tig0023619:45070-45089 None:intergenic 50.0%
ATCGCAAGATTCCTCAAGGC+TGG - tig0023619:45080-45099 Msa1410190:CDS 50.0%
! GTATCTCGCTGCTGAGGTTT+TGG - tig0023619:45160-45179 Msa1410190:CDS 50.0%
!!! CGCTGCTGAGGTTTTGGAAT+TGG - tig0023619:45166-45185 Msa1410190:CDS 50.0%
!!! GCTGAGGTTTTGGAATTGGC+TGG - tig0023619:45170-45189 Msa1410190:CDS 50.0%
! AATTGGCTGGTAATGCTGCG+AGG - tig0023619:45183-45202 Msa1410190:CDS 50.0%
! ATTGGCTGGTAATGCTGCGA+GGG - tig0023619:45184-45203 Msa1410190:CDS 50.0%
!! GACACATTCAGCTTGCTGTG+AGG - tig0023619:45234-45253 Msa1410190:CDS 50.0%
TCCGACGGGGAATTGAAGAC+CGG + tig0023619:45057-45076 None:intergenic 55.0%
! CGGCGTATTTTCCAGCCTTG+AGG + tig0023619:45094-45113 None:intergenic 55.0%
GGAAAATACGCCGAGCGTGT+TGG - tig0023619:45101-45120 Msa1410190:CDS 55.0%
AGAGAGATAGACCGGAGCAC+CGG + tig0023619:45129-45148 None:intergenic 55.0%
TCTCGAGTATCTCGCTGCTG+AGG - tig0023619:45154-45173 Msa1410190:CDS 55.0%
GCCGGTCTTCAATTCCCCGT+CGG - tig0023619:45053-45072 Msa1410190:CDS 60.0%
! TACGCCGAGCGTGTTGGCGC+CGG - tig0023619:45107-45126 Msa1410190:CDS 70.0%
!! AGCACCGGCGCCAACACGCT+CGG + tig0023619:45114-45133 None:intergenic 70.0%
!! GCGTGTTGGCGCCGGTGCTC+CGG - tig0023619:45115-45134 Msa1410190:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0023619 gene 44969 45397 44969 ID=Msa1410190;Name=Msa1410190
tig0023619 mRNA 44969 45397 44969 ID=Msa1410190-mRNA-1;Parent=Msa1410190;Name=Msa1410190-mRNA-1;_AED=0.18;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|142
tig0023619 exon 44969 45397 44969 ID=Msa1410190-mRNA-1:exon:26439;Parent=Msa1410190-mRNA-1
tig0023619 CDS 44969 45397 44969 ID=Msa1410190-mRNA-1:cds;Parent=Msa1410190-mRNA-1
Gene Sequence

>Msa1410190

ATGAGTTCCAAAGGTGCCACAACTACAACCAAAGGAGGAAGAGGCAAACCAAAAGCCTCCAAATCCGTTTCAAGATCTTCAAAAGCCGGTCTTCAATTCCCCGTCGGAAGAATCGCAAGATTCCTCAAGGCTGGAAAATACGCCGAGCGTGTTGGCGCCGGTGCTCCGGTCTATCTCTCTGCTGTTCTCGAGTATCTCGCTGCTGAGGTTTTGGAATTGGCTGGTAATGCTGCGAGGGACAACAAAAAGAATCGAATTGTTCCGAGACACATTCAGCTTGCTGTGAGGAATGATGAAGAACTCAGCAAGCTTTTGGGTGCTGTAACCATTGCTAATGGTGGTGTTTTGCCCAATATTCATCAGACATTGCTTCCTAAGAAGGTTGGAAAAGGGAAAGGTGAAATTGGATCTGCTTCTCAAGAATTTTAG

Protein sequence

>Msa1410190

MSSKGATTTTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF*