AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0737130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0737130 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0737130 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0737130 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0737130 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0737130 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0737130 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0737130 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0737130 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0737130 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0737130 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0737130 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0737130 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0737130 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0737130 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0737130 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0737130 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0737130 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0737130 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0737130 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0737130 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0737130 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0737130 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0737130 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0737130 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0737130 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0737130 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0737130 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0737130 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0737130 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0737130 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0737130 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0737130 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCTCTAACCAATCTT+TGG 0.237540 5_1:+54133903 None:intergenic
TCACGAAGAGCGACTGTTCC+TGG 0.301860 5_1:+54133977 None:intergenic
GCAGCTGAAGCTTATCTTGT+TGG 0.346066 5_1:-54133821 Msa0737130:CDS
TTGAATATCCTTAGGCATAA+TGG 0.349539 5_1:+54133751 None:intergenic
GAAGCTTCCTTTCCAAAGAT+TGG 0.376805 5_1:-54133915 Msa0737130:CDS
CGGAAATCAGCTCCGGCGAC+TGG 0.383526 5_1:-54134028 Msa0737130:CDS
GAGCGGAAACAGCGCTACTC+TGG 0.433957 5_1:+54133852 None:intergenic
AGCTGATTTCCGAGCAGCTT+TGG 0.435458 5_1:+54134039 None:intergenic
TTCTTCACTCCTCCAGTCGC+CGG 0.441465 5_1:+54134016 None:intergenic
AAGCCCTCTCGCCTCTGATT+CGG 0.461144 5_1:+54133720 None:intergenic
TTGGTAGCGAGCTGTTTCCT+TGG 0.465712 5_1:+54134058 None:intergenic
AGGCATAATGGTAACTCTCT+TGG 0.481302 5_1:+54133763 None:intergenic
GGCATGAATAGCACAAAGAT+TGG 0.490509 5_1:+54133784 None:intergenic
CGAGCAAGTTGAATATCCTT+AGG 0.498424 5_1:+54133743 None:intergenic
AAACAGCGCTACTCTGGAAT+CGG 0.503171 5_1:+54133858 None:intergenic
AGAGCACAGAGCTTCTCATA+AGG 0.533669 5_1:-54133937 Msa0737130:CDS
CAACTTGCTCGCCGAATCAG+AGG 0.534605 5_1:-54133731 Msa0737130:CDS
AGCTGCTCGGAAATCAGCTC+CGG 0.536007 5_1:-54134035 Msa0737130:CDS
GAGAGTTACCATTATGCCTA+AGG 0.538018 5_1:-54133759 Msa0737130:CDS
ATTGGTTAGAGAAATTGCTC+AGG 0.542546 5_1:-54133897 Msa0737130:CDS
TCTCTAACCAATCTTTGGAA+AGG 0.542941 5_1:+54133908 None:intergenic
AGCTCGCTACCAAAGCTGCT+CGG 0.565204 5_1:-54134048 Msa0737130:CDS
TTCAGCTGCTTCTTGAAGAG+CGG 0.576635 5_1:+54133835 None:intergenic
TCGCCGAATCAGAGGCGAGA+GGG 0.598725 5_1:-54133723 Msa0737130:CDS
AAATCAGCTCCGGCGACTGG+AGG 0.630058 5_1:-54134025 Msa0737130:CDS
AAGCCACACAGATTCCGTCC+AGG 0.632383 5_1:-54133995 Msa0737130:CDS
CTCGCCGAATCAGAGGCGAG+AGG 0.633846 5_1:-54133724 Msa0737130:CDS
GAAGAGCGACTGTTCCTGGA+CGG 0.668213 5_1:+54133981 None:intergenic
GTTCCTGGACGGAATCTGTG+TGG 0.677012 5_1:+54133992 None:intergenic
CAAACAGCAAGAAAATCCAC+CGG 0.691826 5_1:-54134091 Msa0737130:CDS
ACAGCAAGAAAATCCACCGG+TGG 0.705026 5_1:-54134088 Msa0737130:CDS
CCACCGGTGGAAAAGCACCA+AGG 0.713134 5_1:-54134075 Msa0737130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CAATTTCTCTAACCAATCTT+TGG + chr5_1:54133924-54133943 None:intergenic 30.0%
TTGAATATCCTTAGGCATAA+TGG + chr5_1:54134076-54134095 None:intergenic 30.0%
!!! GGATTTTCTTGCTGTTTGTT+TGG + chr5_1:54133731-54133750 None:intergenic 35.0%
! TCTCTAACCAATCTTTGGAA+AGG + chr5_1:54133919-54133938 None:intergenic 35.0%
ATTGGTTAGAGAAATTGCTC+AGG - chr5_1:54133927-54133946 Msa0737130:CDS 35.0%
CAAACAGCAAGAAAATCCAC+CGG - chr5_1:54133733-54133752 Msa0737130:CDS 40.0%
GAAGCTTCCTTTCCAAAGAT+TGG - chr5_1:54133909-54133928 Msa0737130:CDS 40.0%
GGCATGAATAGCACAAAGAT+TGG + chr5_1:54134043-54134062 None:intergenic 40.0%
AGGCATAATGGTAACTCTCT+TGG + chr5_1:54134064-54134083 None:intergenic 40.0%
GAGAGTTACCATTATGCCTA+AGG - chr5_1:54134065-54134084 Msa0737130:CDS 40.0%
! CGAGCAAGTTGAATATCCTT+AGG + chr5_1:54134084-54134103 None:intergenic 40.0%
!!! TTTCCTTGGTGCTTTTCCAC+CGG + chr5_1:54133755-54133774 None:intergenic 45.0%
AGAGCACAGAGCTTCTCATA+AGG - chr5_1:54133887-54133906 Msa0737130:CDS 45.0%
!! AAACAGCGCTACTCTGGAAT+CGG + chr5_1:54133969-54133988 None:intergenic 45.0%
TTCAGCTGCTTCTTGAAGAG+CGG + chr5_1:54133992-54134011 None:intergenic 45.0%
GCAGCTGAAGCTTATCTTGT+TGG - chr5_1:54134003-54134022 Msa0737130:CDS 45.0%
ACAGCAAGAAAATCCACCGG+TGG - chr5_1:54133736-54133755 Msa0737130:CDS 50.0%
!! TTGGTAGCGAGCTGTTTCCT+TGG + chr5_1:54133769-54133788 None:intergenic 50.0%
! AGCTGATTTCCGAGCAGCTT+TGG + chr5_1:54133788-54133807 None:intergenic 50.0%
AGCTCGCTACCAAAGCTGCT+CGG - chr5_1:54133776-54133795 Msa0737130:CDS 55.0%
AGCTGCTCGGAAATCAGCTC+CGG - chr5_1:54133789-54133808 Msa0737130:CDS 55.0%
TTCTTCACTCCTCCAGTCGC+CGG + chr5_1:54133811-54133830 None:intergenic 55.0%
AAGCCACACAGATTCCGTCC+AGG - chr5_1:54133829-54133848 Msa0737130:CDS 55.0%
GTTCCTGGACGGAATCTGTG+TGG + chr5_1:54133835-54133854 None:intergenic 55.0%
GAAGAGCGACTGTTCCTGGA+CGG + chr5_1:54133846-54133865 None:intergenic 55.0%
TCACGAAGAGCGACTGTTCC+TGG + chr5_1:54133850-54133869 None:intergenic 55.0%
! CAACTTGCTCGCCGAATCAG+AGG - chr5_1:54134093-54134112 Msa0737130:CDS 55.0%
AAGCCCTCTCGCCTCTGATT+CGG + chr5_1:54134107-54134126 None:intergenic 55.0%
!!! CCTTGGTGCTTTTCCACCGG+TGG + chr5_1:54133752-54133771 None:intergenic 60.0%
!! CCACCGGTGGAAAAGCACCA+AGG - chr5_1:54133749-54133768 Msa0737130:CDS 60.0%
AAATCAGCTCCGGCGACTGG+AGG - chr5_1:54133799-54133818 Msa0737130:CDS 60.0%
GAGCGGAAACAGCGCTACTC+TGG + chr5_1:54133975-54133994 None:intergenic 60.0%
TCGCCGAATCAGAGGCGAGA+GGG - chr5_1:54134101-54134120 Msa0737130:CDS 60.0%
CGGAAATCAGCTCCGGCGAC+TGG - chr5_1:54133796-54133815 Msa0737130:CDS 65.0%
CTCGCCGAATCAGAGGCGAG+AGG - chr5_1:54134100-54134119 Msa0737130:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 54133718 54134128 54133718 ID=Msa0737130;Name=Msa0737130
chr5_1 mRNA 54133718 54134128 54133718 ID=Msa0737130-mRNA-1;Parent=Msa0737130;Name=Msa0737130-mRNA-1;_AED=0.36;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|136
chr5_1 exon 54133718 54134128 54133718 ID=Msa0737130-mRNA-1:exon:15238;Parent=Msa0737130-mRNA-1
chr5_1 CDS 54133718 54134128 54133718 ID=Msa0737130-mRNA-1:cds;Parent=Msa0737130-mRNA-1
Gene Sequence

>Msa0737130

ATGGCACGTACCAAACAAACAGCAAGAAAATCCACCGGTGGAAAAGCACCAAGGAAACAGCTCGCTACCAAAGCTGCTCGGAAATCAGCTCCGGCGACTGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACAGTCGCTCTTCGTGAAATCAGAAAGTATCAGAAGAGCACAGAGCTTCTCATAAGGAAGCTTCCTTTCCAAAGATTGGTTAGAGAAATTGCTCAGGATTTCAAAACTGATCTCCGATTCCAGAGTAGCGCTGTTTCCGCTCTTCAAGAAGCAGCTGAAGCTTATCTTGTTGGTTTATTTGAAGATACCAATCTTTGTGCTATTCATGCCAAGAGAGTTACCATTATGCCTAAGGATATTCAACTTGCTCGCCGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>Msa0737130

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*