AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1160830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1160830 MtrunA17_Chr7g0255911 98.969 97 1 0 1 97 1 97 1.53e-63 187
Msa1160830 MtrunA17_Chr3g0139681 74.227 97 25 0 1 97 1 97 3.84e-48 148
Msa1160830 MtrunA17_Chr3g0117191 64.706 85 30 0 1 85 1 85 4.72e-35 115
Msa1160830 MtrunA17_Chr3g0117431 74.359 39 10 0 1 39 1 39 4.18e-15 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1160830 AT1G14980.1 70.103 97 29 0 1 97 1 97 5.30e-47 145
Msa1160830 AT1G23100.1 69.072 97 30 0 1 97 1 97 5.62e-45 140
Msa1160830 AT1G14980.2 70.732 82 24 0 1 82 1 82 8.82e-38 122

Find 46 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCTGGTTTAGTTATTT+TGG 0.048261 7_4:+50943933 Msa1160830:three_prime_UTR
CAAAACATTTCTTAGAAATT+TGG 0.074238 7_4:+50943796 Msa1160830:three_prime_UTR
TCGCTTCGCCATTTCGAAAT+TGG 0.172281 7_4:-50941047 None:intergenic
GTCAAAGTCACTGGCGGATT+TGG 0.271412 7_4:+50943663 Msa1160830:three_prime_UTR
AAAACATTTCTTAGAAATTT+GGG 0.271475 7_4:+50943797 Msa1160830:three_prime_UTR
GTATGGAGATGACGATATAT+TGG 0.325927 7_4:+50943622 Msa1160830:CDS
ATGTTGCTTTAGCTGAATTC+TGG 0.327440 7_4:+50942236 Msa1160830:intron
TCCATTGTTCAACCGTGTTC+TGG 0.339609 7_4:+50941075 Msa1160830:CDS
GCTGTTGGCCCTGGTGTTCA+TGG 0.340171 7_4:+50942269 Msa1160830:CDS
GCTTTAGTAATTTATATACT+AGG 0.371190 7_4:+50943691 Msa1160830:three_prime_UTR
ACAGGCTATGGAATGTAATA+AGG 0.376772 7_4:-50943884 None:intergenic
TCACCTTCCTTTACAGCAAC+AGG 0.376842 7_4:-50942311 None:intergenic
AAAATACTAATCTTGATTGT+TGG 0.377725 7_4:-50943820 None:intergenic
TACATTCCATAGCCTGTTTG+TGG 0.378729 7_4:+50943890 Msa1160830:three_prime_UTR
TTTGGTAGGTATTATCTGTA+TGG 0.394343 7_4:+50943605 Msa1160830:intron
AGTATATAAATTACTAAAGC+AGG 0.420099 7_4:-50943688 None:intergenic
ACTCCTTCCTGTTGCTGTAA+AGG 0.425958 7_4:+50942304 Msa1160830:CDS
AATTTAAATAACCCACAAAC+AGG 0.426429 7_4:-50943902 None:intergenic
CGTTTGCTCCAATTTCGAAA+TGG 0.427778 7_4:+50941039 Msa1160830:exon
TAAATTTAAATGCATTCCTC+TGG 0.431603 7_4:+50943919 Msa1160830:three_prime_UTR
ACCTCAACTCCTCCATATTC+AGG 0.442558 7_4:-50942347 None:intergenic
TATGGAGATGACGATATATT+GGG 0.451979 7_4:+50943623 Msa1160830:CDS
AATAACCCACAAACAGGCTA+TGG 0.510133 7_4:-50943896 None:intergenic
AAAGTTGTTGCTGTTGGCCC+TGG 0.511246 7_4:+50942260 Msa1160830:CDS
ACATTCCATAGCCTGTTTGT+GGG 0.513203 7_4:+50943891 Msa1160830:three_prime_UTR
CCATCCTTACCATGAACACC+AGG 0.526476 7_4:-50942278 None:intergenic
TCTGGAAAAGTTGTTGCTGT+TGG 0.541060 7_4:+50942254 Msa1160830:CDS
CGGTAACAAAATTCCAGCGT+TGG 0.547494 7_4:-50941125 None:intergenic
TGGCCCTGGTGTTCATGGTA+AGG 0.550877 7_4:+50942274 Msa1160830:CDS
GTATATAAATTACTAAAGCA+GGG 0.559702 7_4:-50943687 None:intergenic
CTTCCTGTTGCTGTAAAGGA+AGG 0.582243 7_4:+50942308 Msa1160830:CDS
ATGATTGATGTCAAAGTCAC+TGG 0.587914 7_4:+50943654 Msa1160830:exon
ACCAGAACACGGTTGAACAA+TGG 0.591274 7_4:-50941076 None:intergenic
GCCTGAATATGGAGGAGTTG+AGG 0.598179 7_4:+50942346 Msa1160830:CDS
CCTGGTGTTCATGGTAAGGA+TGG 0.599475 7_4:+50942278 Msa1160830:CDS
TCCATATTCAGGCAAAAGAA+CGG 0.605636 7_4:-50942336 None:intergenic
GTTACCGGAGAAAATCTCCA+AGG 0.620509 7_4:+50941141 Msa1160830:CDS
GTCCAAAATAACTAAACCAG+AGG 0.631096 7_4:-50943935 None:intergenic
CTTCCTTTACAGCAACAGGA+AGG 0.636048 7_4:-50942307 None:intergenic
TATATAAATTACTAAAGCAG+GGG 0.636084 7_4:-50943686 None:intergenic
CAATCTTCTCAACCAGAACA+CGG 0.641660 7_4:-50941087 None:intergenic
GAGCAAACGACAGCAACACA+AGG 0.644728 7_4:-50941025 None:intergenic
GAATGTAATAAGGTGCGTTG+CGG 0.645735 7_4:-50943874 None:intergenic
ATGGTATGTTTGAACATGCA+TGG 0.648776 7_4:-50943993 None:intergenic
ATTGATGTCAAAGTCACTGG+CGG 0.685458 7_4:+50943657 Msa1160830:exon
CATCCTTACCATGAACACCA+GGG 0.736860 7_4:-50942277 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAATCTTAAACATTT+TGG - chr7_4:50941937-50941956 None:intergenic 10.0%
!! TTTATAATTTACATTCTATA+TGG - chr7_4:50943088-50943107 None:intergenic 10.0%
!! TATAGAATGTAAATTATAAA+TGG + chr7_4:50943088-50943107 Msa1160830:intron 10.0%
!!! TGAATTTTTTAATATTTTCA+TGG + chr7_4:50943124-50943143 Msa1160830:intron 10.0%
!! ATAAAAATACATTATACCAA+TGG - chr7_4:50942791-50942810 None:intergenic 15.0%
!! ATAAAAAAATTGAATAGAGA+TGG - chr7_4:50942893-50942912 None:intergenic 15.0%
!! AAAAACTAGGAATTTATTTA+GGG - chr7_4:50943234-50943253 None:intergenic 15.0%
!! CAAAAAAATATGATCTAATT+AGG - chr7_4:50943589-50943608 None:intergenic 15.0%
!!! CTAATTAGATCATATTTTTT+TGG + chr7_4:50943587-50943606 Msa1160830:intron 15.0%
!! AAAACATTTCTTAGAAATTT+GGG + chr7_4:50943797-50943816 Msa1160830:three_prime_UTR 15.0%
!! TCAAAAAACAAACATTTGTT+TGG - chr7_4:50941639-50941658 None:intergenic 20.0%
!!! TTTTGACGTTGTTTATATTT+CGG + chr7_4:50941653-50941672 Msa1160830:intron 20.0%
!! TAGATAATCAAAATTCTACT+AGG - chr7_4:50941802-50941821 None:intergenic 20.0%
!! ATGTATATCATAAAAATGCA+TGG - chr7_4:50942039-50942058 None:intergenic 20.0%
!!! ATAACTTCATAGTCTTAATT+AGG + chr7_4:50942911-50942930 Msa1160830:intron 20.0%
!! TAATTTACATTCTATATGGT+TGG - chr7_4:50943084-50943103 None:intergenic 20.0%
!!! TTTTTAATATTTTCATGGTC+TGG + chr7_4:50943129-50943148 Msa1160830:intron 20.0%
!!! TTTTAATATTTTCATGGTCT+GGG + chr7_4:50943130-50943149 Msa1160830:intron 20.0%
!! TATGATATACATTGAATTGA+AGG + chr7_4:50943171-50943190 Msa1160830:intron 20.0%
!! AAAACTAGGAATTTATTTAG+GGG - chr7_4:50943233-50943252 None:intergenic 20.0%
!! GAAAAACTAGGAATTTATTT+AGG - chr7_4:50943235-50943254 None:intergenic 20.0%
!! TAATCATATTGATATGTAGT+CGG + chr7_4:50943482-50943501 Msa1160830:intron 20.0%
!!! TTAGATCATATTTTTTTGGT+AGG + chr7_4:50943591-50943610 Msa1160830:intron 20.0%
!! TATATAAATTACTAAAGCAG+GGG - chr7_4:50943689-50943708 None:intergenic 20.0%
!! GTATATAAATTACTAAAGCA+GGG - chr7_4:50943690-50943709 None:intergenic 20.0%
!! AGTATATAAATTACTAAAGC+AGG - chr7_4:50943691-50943710 None:intergenic 20.0%
!! GCTTTAGTAATTTATATACT+AGG + chr7_4:50943691-50943710 Msa1160830:three_prime_UTR 20.0%
!!! ACAATCTATATTTTGTTTTC+AGG + chr7_4:50943773-50943792 Msa1160830:three_prime_UTR 20.0%
!! CAAAACATTTCTTAGAAATT+TGG + chr7_4:50943796-50943815 Msa1160830:three_prime_UTR 20.0%
!! AAAATACTAATCTTGATTGT+TGG - chr7_4:50943823-50943842 None:intergenic 20.0%
!!! TAGTATTTTTATGCAACTAT+TGG + chr7_4:50943834-50943853 Msa1160830:three_prime_UTR 20.0%
!!! AGTATTTTTATGCAACTATT+GGG + chr7_4:50943835-50943854 Msa1160830:three_prime_UTR 20.0%
!! TATGTTCTTATCACACTTTT+AGG + chr7_4:50941285-50941304 Msa1160830:intron 25.0%
! ATATTCATCTATTCTGTCTT+AGG + chr7_4:50941374-50941393 Msa1160830:intron 25.0%
! TATTCATCTATTCTGTCTTA+GGG + chr7_4:50941375-50941394 Msa1160830:intron 25.0%
! ATGCAAATAAATAAGCTTTC+AGG + chr7_4:50941441-50941460 Msa1160830:intron 25.0%
! AAACAAACATTTGTTTGGTT+TGG - chr7_4:50941634-50941653 None:intergenic 25.0%
! AATTTGAATGTTCTTGATTC+AGG + chr7_4:50941953-50941972 Msa1160830:intron 25.0%
! ATTTGAATGTTCTTGATTCA+GGG + chr7_4:50941954-50941973 Msa1160830:intron 25.0%
! TGGTTTATGATAGAACTTAA+TGG + chr7_4:50942114-50942133 Msa1160830:intron 25.0%
! TTAATTCATTTAGTCAGTTG+AGG + chr7_4:50942587-50942606 Msa1160830:intron 25.0%
! TAATTCATTTAGTCAGTTGA+GGG + chr7_4:50942588-50942607 Msa1160830:intron 25.0%
!!! CATGAAATCACCTTTTTTTT+CGG + chr7_4:50942622-50942641 Msa1160830:intron 25.0%
!!! AACAGCAAATTTTAACATGA+AGG - chr7_4:50942661-50942680 None:intergenic 25.0%
!!! TAAAATTTGCTGTTGAATGT+TGG + chr7_4:50942667-50942686 Msa1160830:intron 25.0%
! AAACATTGACATACAATATC+AGG - chr7_4:50942749-50942768 None:intergenic 25.0%
! TGATATTGTATGTCAATGTT+TGG + chr7_4:50942748-50942767 Msa1160830:intron 25.0%
! GATATTGTATGTCAATGTTT+GGG + chr7_4:50942749-50942768 Msa1160830:intron 25.0%
!!! ATTTGATTTTATTCCACCAT+TGG + chr7_4:50942772-50942791 Msa1160830:intron 25.0%
! AAAATACATTATACCAATGG+TGG - chr7_4:50942788-50942807 None:intergenic 25.0%
!! ATATCATACATTTTCAGAAC+TGG - chr7_4:50943159-50943178 None:intergenic 25.0%
! TATAAACTTTGTGAAAGAGA+AGG - chr7_4:50943267-50943286 None:intergenic 25.0%
! TGTGAATAAATTAGATGTGA+TGG + chr7_4:50943508-50943527 Msa1160830:intron 25.0%
!!! GTATTTTTATGCAACTATTG+GGG + chr7_4:50943836-50943855 Msa1160830:three_prime_UTR 25.0%
! AATTTAAATAACCCACAAAC+AGG - chr7_4:50943905-50943924 None:intergenic 25.0%
! TAAATTTAAATGCATTCCTC+TGG + chr7_4:50943919-50943938 Msa1160830:three_prime_UTR 25.0%
TTGATTGTTGATCATGACAT+TGG + chr7_4:50941342-50941361 Msa1160830:intron 30.0%
!! TTTGTTTGGTTTGGTAACAA+GGG - chr7_4:50941625-50941644 None:intergenic 30.0%
!! ATTTGTTTGGTTTGGTAACA+AGG - chr7_4:50941626-50941645 None:intergenic 30.0%
!! GCTTCTAGGATTTCTAAAAT+AGG + chr7_4:50941861-50941880 Msa1160830:intron 30.0%
!! CAGGGTATTTTTCGATTTTA+TGG + chr7_4:50941972-50941991 Msa1160830:intron 30.0%
GTTCATTTCATTAGACTGAT+TGG + chr7_4:50941994-50942013 Msa1160830:intron 30.0%
TATGATATACATTCGTGCTT+TGG + chr7_4:50942047-50942066 Msa1160830:intron 30.0%
! ACCTTTTTATGCATCATTTG+CGG - chr7_4:50942520-50942539 None:intergenic 30.0%
AAACTTTCATAATCAGCTTC+AGG - chr7_4:50942569-50942588 None:intergenic 30.0%
CAACTATGTACCGAAAAAAA+AGG - chr7_4:50942635-50942654 None:intergenic 30.0%
ACAAAGCTAGTCCTAATTTA+TGG + chr7_4:50942710-50942729 Msa1160830:intron 30.0%
TATCGTTTCCTCCATAAATT+AGG - chr7_4:50942724-50942743 None:intergenic 30.0%
TCAACAGTATATAAGTGAGA+AGG - chr7_4:50943003-50943022 None:intergenic 30.0%
TTCTCACTTATATACTGTTG+AGG + chr7_4:50943002-50943021 Msa1160830:intron 30.0%
TCTCACTTATATACTGTTGA+GGG + chr7_4:50943003-50943022 Msa1160830:intron 30.0%
TTTGGTAGGTATTATCTGTA+TGG + chr7_4:50943605-50943624 Msa1160830:intron 30.0%
TATGGAGATGACGATATATT+GGG + chr7_4:50943623-50943642 Msa1160830:CDS 30.0%
!! TTCCTCTGGTTTAGTTATTT+TGG + chr7_4:50943933-50943952 Msa1160830:three_prime_UTR 30.0%
! AAAACCTTGGAGATTTTCTC+CGG - chr7_4:50941148-50941167 None:intergenic 35.0%
AGAGGAGAATGAAAAAACCT+TGG - chr7_4:50941161-50941180 None:intergenic 35.0%
!! ATGGGTATGAATGGATTAAG+AGG - chr7_4:50941179-50941198 None:intergenic 35.0%
AAGAAAAACGAAGACCCAAA+AGG - chr7_4:50941212-50941231 None:intergenic 35.0%
ATTAAAAGACCAATCCAGAC+AGG + chr7_4:50941599-50941618 Msa1160830:intron 35.0%
! CAATTGAATTTTACACCGCA+AGG + chr7_4:50941739-50941758 Msa1160830:intron 35.0%
!! CCTTAATTTGTTCTACCTTG+CGG - chr7_4:50941757-50941776 None:intergenic 35.0%
CGCAAGGTAGAACAAATTAA+GGG + chr7_4:50941755-50941774 Msa1160830:intron 35.0%
AAGGTAGAACAAATTAAGGG+TGG + chr7_4:50941758-50941777 Msa1160830:intron 35.0%
GAAAAGGTTTACTAGGAACT+AGG - chr7_4:50941831-50941850 None:intergenic 35.0%
AGCTCAAGAAAAGGTTTACT+AGG - chr7_4:50941838-50941857 None:intergenic 35.0%
AGAAGCAAAAGCTCAAGAAA+AGG - chr7_4:50941847-50941866 None:intergenic 35.0%
! TTTCTTGAGCTTTTGCTTCT+AGG + chr7_4:50941847-50941866 Msa1160830:intron 35.0%
GAAAGATCTAGAGATAGAGT+TGG + chr7_4:50942082-50942101 Msa1160830:intron 35.0%
GATAGAGTTGGATCGAAATA+TGG + chr7_4:50942094-50942113 Msa1160830:intron 35.0%
!! ATGTTGCTTTAGCTGAATTC+TGG + chr7_4:50942236-50942255 Msa1160830:intron 35.0%
TCCATATTCAGGCAAAAGAA+CGG - chr7_4:50942339-50942358 None:intergenic 35.0%
CTTAATCATGACCTATCTGA+TGG + chr7_4:50942441-50942460 Msa1160830:intron 35.0%
AAGCTAGTCCTAATTTATGG+AGG + chr7_4:50942713-50942732 Msa1160830:intron 35.0%
AGGATTGCACAAGAAAAACT+AGG - chr7_4:50943247-50943266 None:intergenic 35.0%
AACAAACCGCTATTATTCTG+CGG + chr7_4:50943351-50943370 Msa1160830:intron 35.0%
AACATACCGCAGAATAATAG+CGG - chr7_4:50943360-50943379 None:intergenic 35.0%
TCAAATTGCAGCTATAGCAT+TGG + chr7_4:50943395-50943414 Msa1160830:intron 35.0%
TTGCAGAACGTAGAAAATAG+TGG - chr7_4:50943444-50943463 None:intergenic 35.0%
GTATGGAGATGACGATATAT+TGG + chr7_4:50943622-50943641 Msa1160830:CDS 35.0%
ATGATTGATGTCAAAGTCAC+TGG + chr7_4:50943654-50943673 Msa1160830:exon 35.0%
!!! TATTGGGGAACCTTTTTTGA+AGG + chr7_4:50943851-50943870 Msa1160830:three_prime_UTR 35.0%
ACAGGCTATGGAATGTAATA+AGG - chr7_4:50943887-50943906 None:intergenic 35.0%
GTCCAAAATAACTAAACCAG+AGG - chr7_4:50943938-50943957 None:intergenic 35.0%
CGTTTGCTCCAATTTCGAAA+TGG + chr7_4:50941039-50941058 Msa1160830:exon 40.0%
CAATCTTCTCAACCAGAACA+CGG - chr7_4:50941090-50941109 None:intergenic 40.0%
!!! ATTCCAGCGTTGGTTTTTGA+TGG - chr7_4:50941118-50941137 None:intergenic 40.0%
! CAACGCTGGAATTTTGTTAC+CGG + chr7_4:50941126-50941145 Msa1160830:CDS 40.0%
GGAGATGAAATGGGTATGAA+TGG - chr7_4:50941188-50941207 None:intergenic 40.0%
CCAAAAGGTGGAGATGAAAT+GGG - chr7_4:50941197-50941216 None:intergenic 40.0%
! CCATTTCATCTCCACCTTTT+GGG + chr7_4:50941195-50941214 Msa1160830:intron 40.0%
AAAAACGAAGACCCAAAAGG+TGG - chr7_4:50941209-50941228 None:intergenic 40.0%
! ATTCTGTCTTAGGGCTTGTT+TGG + chr7_4:50941384-50941403 Msa1160830:intron 40.0%
CCGCAAGGTAGAACAAATTA+AGG + chr7_4:50941754-50941773 Msa1160830:intron 40.0%
TCTGGAAAAGTTGTTGCTGT+TGG + chr7_4:50942254-50942273 Msa1160830:CDS 40.0%
! ACCGTTCTTTTGCCTGAATA+TGG + chr7_4:50942335-50942354 Msa1160830:CDS 40.0%
! GTTCTTTTGCCTGAATATGG+AGG + chr7_4:50942338-50942357 Msa1160830:CDS 40.0%
AGAAACAGTGTCCATCAGAT+AGG - chr7_4:50942455-50942474 None:intergenic 40.0%
GCCGCAAATGATGCATAAAA+AGG + chr7_4:50942516-50942535 Msa1160830:intron 40.0%
CTTCAGGACTACTACTCAAA+CGG - chr7_4:50942553-50942572 None:intergenic 40.0%
GGTGATTTCATGCGTAGTAT+AGG - chr7_4:50942614-50942633 None:intergenic 40.0%
GGAATTTATTTAGGGGCCAA+GGG - chr7_4:50943226-50943245 None:intergenic 40.0%
AGGAATTTATTTAGGGGCCA+AGG - chr7_4:50943227-50943246 None:intergenic 40.0%
GCTATAGCACTATAGCATAG+CGG + chr7_4:50943322-50943341 Msa1160830:intron 40.0%
ATTGATGTCAAAGTCACTGG+CGG + chr7_4:50943657-50943676 Msa1160830:exon 40.0%
GAATGTAATAAGGTGCGTTG+CGG - chr7_4:50943877-50943896 None:intergenic 40.0%
TACATTCCATAGCCTGTTTG+TGG + chr7_4:50943890-50943909 Msa1160830:three_prime_UTR 40.0%
ACATTCCATAGCCTGTTTGT+GGG + chr7_4:50943891-50943910 Msa1160830:three_prime_UTR 40.0%
AATAACCCACAAACAGGCTA+TGG - chr7_4:50943899-50943918 None:intergenic 40.0%
TCGCTTCGCCATTTCGAAAT+TGG - chr7_4:50941050-50941069 None:intergenic 45.0%
TCCATTGTTCAACCGTGTTC+TGG + chr7_4:50941075-50941094 Msa1160830:CDS 45.0%
ACCAGAACACGGTTGAACAA+TGG - chr7_4:50941079-50941098 None:intergenic 45.0%
CGGTAACAAAATTCCAGCGT+TGG - chr7_4:50941128-50941147 None:intergenic 45.0%
GTTACCGGAGAAAATCTCCA+AGG + chr7_4:50941141-50941160 Msa1160830:CDS 45.0%
! CCCATTTCATCTCCACCTTT+TGG + chr7_4:50941194-50941213 Msa1160830:intron 45.0%
CCCAAAAGGTGGAGATGAAA+TGG - chr7_4:50941198-50941217 None:intergenic 45.0%
ATGTAGCCGATCTCACTTAG+CGG + chr7_4:50942150-50942169 Msa1160830:intron 45.0%
CTTATCCCGCTAAGTGAGAT+CGG - chr7_4:50942159-50942178 None:intergenic 45.0%
CATCCTTACCATGAACACCA+GGG - chr7_4:50942280-50942299 None:intergenic 45.0%
ACTCCTTCCTGTTGCTGTAA+AGG + chr7_4:50942304-50942323 Msa1160830:CDS 45.0%
CTTCCTTTACAGCAACAGGA+AGG - chr7_4:50942310-50942329 None:intergenic 45.0%
CTTCCTGTTGCTGTAAAGGA+AGG + chr7_4:50942308-50942327 Msa1160830:CDS 45.0%
TCACCTTCCTTTACAGCAAC+AGG - chr7_4:50942314-50942333 None:intergenic 45.0%
ACCTCAACTCCTCCATATTC+AGG - chr7_4:50942350-50942369 None:intergenic 45.0%
AGACATGAAGCTCTGCATCA+AGG - chr7_4:50942847-50942866 None:intergenic 45.0%
! AGCTTCATGTCTGAAGCTGA+GGG + chr7_4:50942855-50942874 Msa1160830:intron 45.0%
ATTTATTTAGGGGCCAAGGG+AGG - chr7_4:50943223-50943242 None:intergenic 45.0%
! GCTATAGCATTGGAGCATAG+CGG + chr7_4:50943405-50943424 Msa1160830:intron 45.0%
GAGCAAACGACAGCAACACA+AGG - chr7_4:50941028-50941047 None:intergenic 50.0%
!!! CCAGCGTTGGTTTTTGATGG+AGG - chr7_4:50941115-50941134 None:intergenic 50.0%
CCTCCATCAAAAACCAACGC+TGG + chr7_4:50941112-50941131 Msa1160830:CDS 50.0%
TAACAAGGGCCTGTCTGGAT+TGG - chr7_4:50941611-50941630 None:intergenic 50.0%
! TTTGGTAACAAGGGCCTGTC+TGG - chr7_4:50941616-50941635 None:intergenic 50.0%
GCTAAGTGAGATCGGCTACA+TGG - chr7_4:50942151-50942170 None:intergenic 50.0%
TGTAGCCGATCTCACTTAGC+GGG + chr7_4:50942151-50942170 Msa1160830:intron 50.0%
! AAAGTTGTTGCTGTTGGCCC+TGG + chr7_4:50942260-50942279 Msa1160830:CDS 50.0%
CCATCCTTACCATGAACACC+AGG - chr7_4:50942281-50942300 None:intergenic 50.0%
CCTGGTGTTCATGGTAAGGA+TGG + chr7_4:50942278-50942297 Msa1160830:CDS 50.0%
GCCTGAATATGGAGGAGTTG+AGG + chr7_4:50942346-50942365 Msa1160830:CDS 50.0%
! GAGCTTCATGTCTGAAGCTG+AGG + chr7_4:50942854-50942873 Msa1160830:intron 50.0%
!! GTCAAAGTCACTGGCGGATT+TGG + chr7_4:50943663-50943682 Msa1160830:three_prime_UTR 50.0%
TGCGTTGCGGCCTTCAAAAA+AGG - chr7_4:50943864-50943883 None:intergenic 50.0%
TGGCCCTGGTGTTCATGGTA+AGG + chr7_4:50942274-50942293 Msa1160830:CDS 55.0%
TCGCAAGATGCATCCTCCCT+TGG + chr7_4:50943207-50943226 Msa1160830:intron 55.0%
GCTGTTGGCCCTGGTGTTCA+TGG + chr7_4:50942269-50942288 Msa1160830:CDS 60.0%
! ATGTCTGAAGCTGAGGGCGC+AGG + chr7_4:50942861-50942880 Msa1160830:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 50941007 50944008 50941007 ID=Msa1160830;Name=Msa1160830
chr7_4 mRNA 50941007 50944008 50941007 ID=Msa1160830-mRNA-1;Parent=Msa1160830;Name=Msa1160830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=51|1|1|1|0.5|0.33|3|347|97
chr7_4 exon 50941007 50941162 50941007 ID=Msa1160830-mRNA-1:exon:14263;Parent=Msa1160830-mRNA-1
chr7_4 exon 50942248 50942387 50942248 ID=Msa1160830-mRNA-1:exon:14264;Parent=Msa1160830-mRNA-1
chr7_4 exon 50943613 50944008 50943613 ID=Msa1160830-mRNA-1:exon:14265;Parent=Msa1160830-mRNA-1
chr7_4 five_prime_UTR 50941007 50941057 50941007 ID=Msa1160830-mRNA-1:five_prime_utr;Parent=Msa1160830-mRNA-1
chr7_4 CDS 50941058 50941162 50941058 ID=Msa1160830-mRNA-1:cds;Parent=Msa1160830-mRNA-1
chr7_4 CDS 50942248 50942387 50942248 ID=Msa1160830-mRNA-1:cds;Parent=Msa1160830-mRNA-1
chr7_4 CDS 50943613 50943661 50943613 ID=Msa1160830-mRNA-1:cds;Parent=Msa1160830-mRNA-1
chr7_4 three_prime_UTR 50943662 50944008 50943662 ID=Msa1160830-mRNA-1:three_prime_utr;Parent=Msa1160830-mRNA-1
Gene Sequence

>Msa1160830

ATGGCGAAGCGATTGATTCCATTGTTCAACCGTGTTCTGGTTGAGAAGATTGTTCCTCCATCAAAAACCAACGCTGGAATTTTGTTACCGGAGAAAATCTCCAAGCTGAATTCTGGAAAAGTTGTTGCTGTTGGCCCTGGTGTTCATGGTAAGGATGGCAAACTCCTTCCTGTTGCTGTAAAGGAAGGTGACACCGTTCTTTTGCCTGAATATGGAGGAGTTGAGGTGAAGCTTGATCACAAAGAGTATTATCTGTATGGAGATGACGATATATTGGGAACACTGCATGATTGA
Protein sequence

>Msa1160830

MAKRLIPLFNRVLVEKIVPPSKTNAGILLPEKISKLNSGKVVAVGPGVHGKDGKLLPVAVKEGDTVLLPEYGGVEVKLDHKEYYLYGDDDILGTLHD*