AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1163690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163690 MtrunA17_Chr7g0260991 97.826 138 3 0 1 138 1 138 1.88e-99 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163690 AT1G48160.1 69.369 111 34 0 1 111 1 111 1.35e-56 173
Msa1163690 AT1G48160.2 69.369 111 34 0 1 111 1 111 1.35e-56 173

Find 61 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAGATCTCAACTCGATTT+TGG 0.165471 7_4:-55033808 None:intergenic
TTGCCCAAGCCAAGCAATTT+TGG 0.195524 7_4:+55033719 Msa1163690:three_prime_UTR
TCCTTCAGCAACAGTCTTCT+TGG 0.199519 7_4:-55031232 None:intergenic
TGGTCCTTCCATGGTGTTTA+GGG 0.204157 7_4:-55033293 None:intergenic
AACCCTACTTGTGTCGAAAT+TGG 0.211223 7_4:+55031285 Msa1163690:CDS
TGATGCAGTCTCCTGCTTCT+TGG 0.227052 7_4:-55033313 None:intergenic
TTGGTCCTTCCATGGTGTTT+AGG 0.258012 7_4:-55033294 None:intergenic
TTCTTGGAATTGATGTAAAC+AGG 0.310953 7_4:-55031216 None:intergenic
GGTACAACTTCAATTTAGTT+AGG 0.333994 7_4:-55033525 None:intergenic
TCATGCAAAGAGGACGAGTT+AGG 0.334941 7_4:+55031466 Msa1163690:CDS
ACCCTACTTGTGTCGAAATT+GGG 0.338535 7_4:+55031286 Msa1163690:CDS
GCTGGACCTTCCAACAAATC+TGG 0.347799 7_4:+55033351 Msa1163690:CDS
GAAGTTGTACCACTCGGAAA+TGG 0.352593 7_4:+55033537 Msa1163690:three_prime_UTR
TCCACCTTTCCCAGATTTGT+TGG 0.371350 7_4:-55033361 None:intergenic
TGTGTTTCTTCTTGCAACTT+TGG 0.395224 7_4:-55033645 None:intergenic
GCAACCAAAATTGCTTGGCT+TGG 0.398800 7_4:-55033723 None:intergenic
CCTGCTTCTTGGTCCTTCCA+TGG 0.417818 7_4:-55033302 None:intergenic
CAACCAAAATTGCTTGGCTT+GGG 0.418065 7_4:-55033722 None:intergenic
CTTTGGTTGCCCTTAGCAGT+AGG 0.422768 7_4:+55033746 Msa1163690:three_prime_UTR
TGTTTATTACAGTTTCATAC+TGG 0.431944 7_4:+55033469 Msa1163690:three_prime_UTR
CCTTTCCCAGATTTGTTGGA+AGG 0.448852 7_4:-55033357 None:intergenic
TCAACAACAACTGCAACTGC+TGG 0.454659 7_4:+55033333 Msa1163690:CDS
AAGAAGAAACACATCACGTT+TGG 0.470199 7_4:+55033655 Msa1163690:three_prime_UTR
AAGTTGTACCACTCGGAAAT+GGG 0.471619 7_4:+55033538 Msa1163690:three_prime_UTR
CATGCAAAGAGGACGAGTTA+GGG 0.478286 7_4:+55031467 Msa1163690:CDS
AACCTCACCTCGATTGCAAA+AGG 0.487649 7_4:-55031333 None:intergenic
ACGATCCATCTCTTTATGCT+TGG 0.492762 7_4:-55031186 None:intergenic
GTTGCTGAAGGAAGAAGAAT+CGG 0.496341 7_4:+55031243 Msa1163690:CDS
CTGGACCTTCCAACAAATCT+GGG 0.500340 7_4:+55033352 Msa1163690:CDS
TTTGGTTGCCCTTAGCAGTA+GGG 0.512297 7_4:+55033747 Msa1163690:three_prime_UTR
CAAAGGCAACCAAAATTGCT+TGG 0.513584 7_4:-55033728 None:intergenic
CTTGGTCGTCCCTACTGCTA+AGG 0.514672 7_4:-55033756 None:intergenic
CCCCAATTTCGACACAAGTA+GGG 0.516511 7_4:-55031287 None:intergenic
CCATGGAAGGACCAAGAAGC+AGG 0.522661 7_4:+55033302 Msa1163690:CDS
CCCTACTTGTGTCGAAATTG+GGG 0.523869 7_4:+55031287 Msa1163690:CDS
AATGTTACGCGTTGCAGAGA+TGG 0.526719 7_4:+55033269 Msa1163690:CDS
TCCCCAATTTCGACACAAGT+AGG 0.535068 7_4:-55031288 None:intergenic
CTCTTAGCAAAACTCATACT+CGG 0.542496 7_4:-55033444 None:intergenic
TGATTTCACAAAGTGTAAGA+TGG 0.554466 7_4:+55031152 Msa1163690:exon
TCCAAGAAGACTGTTGCTGA+AGG 0.561279 7_4:+55031231 Msa1163690:CDS
TTGGTCGTCCCTACTGCTAA+GGG 0.563436 7_4:-55033755 None:intergenic
CCTCGTGATTTCATGCAAAG+AGG 0.566493 7_4:+55031456 Msa1163690:CDS
AACTTACTGGAAGCTATGGA+TGG 0.567619 7_4:-55031525 None:intergenic
TCCAACAAATCTGGGAAAGG+TGG 0.573312 7_4:+55033360 Msa1163690:CDS
AGAAACACATCACGTTTGGA+AGG 0.593562 7_4:+55033659 Msa1163690:three_prime_UTR
TTCACAAAGTGTAAGATGGA+AGG 0.594062 7_4:+55031156 Msa1163690:exon
GTCTTGTAGTAGCAAAACCT+TGG 0.597031 7_4:-55033774 None:intergenic
AATTACCAAGCATAAAGAGA+TGG 0.602608 7_4:+55031181 Msa1163690:CDS
CCTTCCAACAAATCTGGGAA+AGG 0.605602 7_4:+55033357 Msa1163690:CDS
TTGTTGATCACAGCTTGACA+AGG 0.619387 7_4:+55031428 Msa1163690:intron
TGGTCCCTAAACACCATGGA+AGG 0.619588 7_4:+55033289 Msa1163690:CDS
CCTCTTTGCATGAAATCACG+AGG 0.620436 7_4:-55031456 None:intergenic
TCAGATCTATTACTCTCATG+AGG 0.629731 7_4:-55031132 None:intergenic
TCTTGGAATTGATGTAAACA+GGG 0.634146 7_4:-55031215 None:intergenic
TAAATTGAAGTTGTACCACT+CGG 0.647405 7_4:+55033531 Msa1163690:three_prime_UTR
AGCAAAACTCATACTCGGAG+AGG 0.666325 7_4:-55033439 None:intergenic
CTACTGCTAAGGGCAACCAA+AGG 0.673967 7_4:-55033745 None:intergenic
CTTAGCAGTAGGGACGACCA+AGG 0.689734 7_4:+55033757 Msa1163690:three_prime_UTR
GAGATGGTCCCTAAACACCA+TGG 0.690946 7_4:+55033285 Msa1163690:CDS
CTCTTTGCATGAAATCACGA+GGG 0.694337 7_4:-55031455 None:intergenic
GCGCAAAACCCATTTCCGAG+TGG 0.747399 7_4:-55033546 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTATGTTAAATAAAA+TGG - chr7_4:55032051-55032070 None:intergenic 10.0%
!! TTTGAATATAGATTTAAAAA+GGG - chr7_4:55032324-55032343 None:intergenic 10.0%
!!! TTTTGAATATAGATTTAAAA+AGG - chr7_4:55032325-55032344 None:intergenic 10.0%
!! AATTAATTATTAGAGAAAAA+GGG - chr7_4:55032618-55032637 None:intergenic 10.0%
!! CAATTAATTATTAGAGAAAA+AGG - chr7_4:55032619-55032638 None:intergenic 15.0%
!!! TCTCTTATTTTTTTTTTTGA+GGG + chr7_4:55033835-55033854 Msa1163690:three_prime_UTR 15.0%
!! ATATTGAAGAAAAAACTTAC+TGG - chr7_4:55031541-55031560 None:intergenic 20.0%
!!! CTTATAATATTCCACTTTTA+TGG + chr7_4:55031920-55031939 Msa1163690:intron 20.0%
!! TTCTTTCTTAGAAATGAAAT+TGG + chr7_4:55032165-55032184 Msa1163690:intron 20.0%
!!! TTACAAATTCAAGTTGATTA+AGG + chr7_4:55032385-55032404 Msa1163690:intron 20.0%
!! ATTAATTGTAAGATCTGAAA+TGG + chr7_4:55032631-55032650 Msa1163690:intron 20.0%
!! ATAAAAAGAATAGCATAATG+TGG - chr7_4:55032768-55032787 None:intergenic 20.0%
!!! TTATGATAGCTTATTTCTTA+TGG + chr7_4:55033071-55033090 Msa1163690:intron 20.0%
!!! CTCTCTTATTTTTTTTTTTG+AGG + chr7_4:55033834-55033853 Msa1163690:three_prime_UTR 20.0%
!!! CTCTTATTTTTTTTTTTGAG+GGG + chr7_4:55033836-55033855 Msa1163690:three_prime_UTR 20.0%
! TAATATAGTTCTTAGCTATC+TGG + chr7_4:55031599-55031618 Msa1163690:intron 25.0%
!! AGAACACATTTTATGGAAAT+GGG - chr7_4:55031690-55031709 None:intergenic 25.0%
!! TAGAACACATTTTATGGAAA+TGG - chr7_4:55031691-55031710 None:intergenic 25.0%
! AAGATATCAAAGTAATGTTG+TGG - chr7_4:55031715-55031734 None:intergenic 25.0%
!! ACATTGATTATACTTTCTAC+CGG + chr7_4:55031892-55031911 Msa1163690:intron 25.0%
!!! ATGCTACTTTAGTTAAAACA+AGG - chr7_4:55032419-55032438 None:intergenic 25.0%
!!! AACTACTTTAGAGTTTGATA+AGG - chr7_4:55032657-55032676 None:intergenic 25.0%
! AGACCATGATAATATAACAT+TGG + chr7_4:55032693-55032712 Msa1163690:intron 25.0%
! AAAAGAATAGCATAATCTTG+TGG - chr7_4:55032719-55032738 None:intergenic 25.0%
! AGACCATGATAATATAACAT+TGG + chr7_4:55032693-55032712 Msa1163690:intron 25.0%
!! TTCATGAAAGCATAAGAATT+GGG + chr7_4:55032942-55032961 Msa1163690:intron 25.0%
! TATATTGTTATGATTTCCAG+CGG - chr7_4:55033010-55033029 None:intergenic 25.0%
!!! TTTTGTTTTTATGTCTTTGC+AGG + chr7_4:55033237-55033256 Msa1163690:intron 25.0%
! TGTTTATTACAGTTTCATAC+TGG + chr7_4:55033469-55033488 Msa1163690:three_prime_UTR 25.0%
TGATTTCACAAAGTGTAAGA+TGG + chr7_4:55031152-55031171 Msa1163690:exon 30.0%
AATTACCAAGCATAAAGAGA+TGG + chr7_4:55031181-55031200 Msa1163690:CDS 30.0%
TCTTGGAATTGATGTAAACA+GGG - chr7_4:55031218-55031237 None:intergenic 30.0%
TTCTTGGAATTGATGTAAAC+AGG - chr7_4:55031219-55031238 None:intergenic 30.0%
AAAAAACTTACTGGAAGCTA+TGG - chr7_4:55031532-55031551 None:intergenic 30.0%
! GAACACATTTTATGGAAATG+GGG - chr7_4:55031689-55031708 None:intergenic 30.0%
! TGTGGTTAGAACACATTTTA+TGG - chr7_4:55031697-55031716 None:intergenic 30.0%
TTGTAATGACAAAGTGATAC+TGG + chr7_4:55031820-55031839 Msa1163690:intron 30.0%
TGGAATATTATAAGTAGTGC+CGG - chr7_4:55031914-55031933 None:intergenic 30.0%
! TCACGTATAAGTTCTAATTG+TGG + chr7_4:55031972-55031991 Msa1163690:intron 30.0%
! GTATAAGTTCTAATTGTGGA+TGG + chr7_4:55031976-55031995 Msa1163690:intron 30.0%
!!! GTTAGACTTTTGTTTCTGTT+TGG + chr7_4:55032025-55032044 Msa1163690:intron 30.0%
!! AGTTAATGATTTGCTCACAT+AGG + chr7_4:55032087-55032106 Msa1163690:intron 30.0%
AATGTTACATGCATAAGGTA+TGG - chr7_4:55032220-55032239 None:intergenic 30.0%
TGCATAATGTTACATGCATA+AGG - chr7_4:55032225-55032244 None:intergenic 30.0%
!!! CTCCAATATTTTGACTGTTT+AGG + chr7_4:55032350-55032369 Msa1163690:intron 30.0%
AAAGCACAAATTGAAGAAGT+AGG + chr7_4:55032445-55032464 Msa1163690:intron 30.0%
TGTTAATAACATCACAACAG+AGG + chr7_4:55032489-55032508 Msa1163690:intron 30.0%
GTTAATAACATCACAACAGA+GGG + chr7_4:55032490-55032509 Msa1163690:intron 30.0%
TGGCCAATGTTATATTATCA+TGG - chr7_4:55032699-55032718 None:intergenic 30.0%
TGGCCAATGTTATATTATCA+TGG - chr7_4:55032699-55032718 None:intergenic 30.0%
TTTAAGCCTGCAGAAATTAT+AGG + chr7_4:55032806-55032825 Msa1163690:intron 30.0%
CTAAATCCTATAATTTCTGC+AGG - chr7_4:55032815-55032834 None:intergenic 30.0%
CTGCAGAAATTATAGGATTT+AGG + chr7_4:55032813-55032832 Msa1163690:intron 30.0%
CAATTCTTATGCTTTCATGA+AGG - chr7_4:55032943-55032962 None:intergenic 30.0%
! CTTCATGAAAGCATAAGAAT+TGG + chr7_4:55032941-55032960 Msa1163690:intron 30.0%
ATATTGTTATGATTTCCAGC+GGG - chr7_4:55033009-55033028 None:intergenic 30.0%
AATATCATAGATGATCTCTC+TGG - chr7_4:55033046-55033065 None:intergenic 30.0%
TGCAAGCATCACATATTAAA+AGG + chr7_4:55033130-55033149 Msa1163690:intron 30.0%
! CTTGTTGAACATTTTCATAC+TGG + chr7_4:55033414-55033433 Msa1163690:three_prime_UTR 30.0%
GGTACAACTTCAATTTAGTT+AGG - chr7_4:55033528-55033547 None:intergenic 30.0%
TAAATTGAAGTTGTACCACT+CGG + chr7_4:55033531-55033550 Msa1163690:three_prime_UTR 30.0%
! TTAAGATCTCAACTCGATTT+TGG - chr7_4:55033811-55033830 None:intergenic 30.0%
TCAGATCTATTACTCTCATG+AGG - chr7_4:55031135-55031154 None:intergenic 35.0%
TTCACAAAGTGTAAGATGGA+AGG + chr7_4:55031156-55031175 Msa1163690:exon 35.0%
! TAAACTTCCTTTTGCAATCG+AGG + chr7_4:55031326-55031345 Msa1163690:CDS 35.0%
! AGTTAGGGTTTTGCTCAAAA+AGG + chr7_4:55031482-55031501 Msa1163690:CDS 35.0%
! TTTTGCTCAAAAAGGAGGAT+GGG + chr7_4:55031490-55031509 Msa1163690:CDS 35.0%
!! AAGTGATACTGGCTTTAAGT+TGG + chr7_4:55031831-55031850 Msa1163690:intron 35.0%
!! AGTGATACTGGCTTTAAGTT+GGG + chr7_4:55031832-55031851 Msa1163690:intron 35.0%
!! ATATTCCACTTTTATGGCTC+TGG + chr7_4:55031926-55031945 Msa1163690:intron 35.0%
TCTTCTCCATTTCTTGTCTT+GGG - chr7_4:55032193-55032212 None:intergenic 35.0%
GATCAACATAGAAGATACGT+AGG + chr7_4:55032249-55032268 Msa1163690:intron 35.0%
! ACGTAGGAACTACATGTTTT+TGG + chr7_4:55032265-55032284 Msa1163690:intron 35.0%
GGCCTAAACAGTCAAAATAT+TGG - chr7_4:55032355-55032374 None:intergenic 35.0%
!! TGAATTTGTAAAAGTGCCAC+TGG - chr7_4:55032376-55032395 None:intergenic 35.0%
! GTAAAACTAGTTTGCACTGA+CGG + chr7_4:55032585-55032604 Msa1163690:intron 35.0%
!! TTTCATGAAGGCGTTTGAAT+TGG - chr7_4:55032931-55032950 None:intergenic 35.0%
CTCTTAGCAAAACTCATACT+CGG - chr7_4:55033447-55033466 None:intergenic 35.0%
! GGATGTAACATTTTGCTAAG+AGG + chr7_4:55033572-55033591 Msa1163690:three_prime_UTR 35.0%
TGTGTTTCTTCTTGCAACTT+TGG - chr7_4:55033648-55033667 None:intergenic 35.0%
AAGAAGAAACACATCACGTT+TGG + chr7_4:55033655-55033674 Msa1163690:three_prime_UTR 35.0%
ACGATCCATCTCTTTATGCT+TGG - chr7_4:55031189-55031208 None:intergenic 40.0%
GTTGCTGAAGGAAGAAGAAT+CGG + chr7_4:55031243-55031262 Msa1163690:CDS 40.0%
! AGTAGGGTTTTCGCATGATT+TGG - chr7_4:55031274-55031293 None:intergenic 40.0%
AACCCTACTTGTGTCGAAAT+TGG + chr7_4:55031285-55031304 Msa1163690:CDS 40.0%
ACCCTACTTGTGTCGAAATT+GGG + chr7_4:55031286-55031305 Msa1163690:CDS 40.0%
TTGTTGATCACAGCTTGACA+AGG + chr7_4:55031428-55031447 Msa1163690:intron 40.0%
CTCTTTGCATGAAATCACGA+GGG - chr7_4:55031458-55031477 None:intergenic 40.0%
! TAGGGTTTTGCTCAAAAAGG+AGG + chr7_4:55031485-55031504 Msa1163690:CDS 40.0%
! GTTTTGCTCAAAAAGGAGGA+TGG + chr7_4:55031489-55031508 Msa1163690:CDS 40.0%
AACTTACTGGAAGCTATGGA+TGG - chr7_4:55031528-55031547 None:intergenic 40.0%
! TCATTCCTTTTCTAAGCCAG+AGG + chr7_4:55031660-55031679 Msa1163690:intron 40.0%
! CACATTTTATGGAAATGGGG+TGG - chr7_4:55031686-55031705 None:intergenic 40.0%
! GGATGGACTTAGAGTAGAAT+TGG + chr7_4:55031993-55032012 Msa1163690:intron 40.0%
TTGGATCCCAAGACAAGAAA+TGG + chr7_4:55032184-55032203 Msa1163690:intron 40.0%
CTCTTCTCCATTTCTTGTCT+TGG - chr7_4:55032194-55032213 None:intergenic 40.0%
!!! ATTTTGACTGTTTAGGCCAG+TGG + chr7_4:55032357-55032376 Msa1163690:intron 40.0%
TAAGAATTGGGATGAACCTG+AGG + chr7_4:55032954-55032973 Msa1163690:intron 40.0%
AAGAATTGGGATGAACCTGA+GGG + chr7_4:55032955-55032974 Msa1163690:intron 40.0%
ATAGATGATCTCTCTGGTGT+GGG - chr7_4:55033040-55033059 None:intergenic 40.0%
! CGAGTATGAGTTTTGCTAAG+AGG + chr7_4:55033445-55033464 Msa1163690:three_prime_UTR 40.0%
AAGTTGTACCACTCGGAAAT+GGG + chr7_4:55033538-55033557 Msa1163690:three_prime_UTR 40.0%
AGAAACACATCACGTTTGGA+AGG + chr7_4:55033659-55033678 Msa1163690:three_prime_UTR 40.0%
CAACCAAAATTGCTTGGCTT+GGG - chr7_4:55033725-55033744 None:intergenic 40.0%
CAAAGGCAACCAAAATTGCT+TGG - chr7_4:55033731-55033750 None:intergenic 40.0%
!!! CAAGCAATTTTGGTTGCCTT+TGG + chr7_4:55033729-55033748 Msa1163690:three_prime_UTR 40.0%
GTCTTGTAGTAGCAAAACCT+TGG - chr7_4:55033777-55033796 None:intergenic 40.0%
TCCAAGAAGACTGTTGCTGA+AGG + chr7_4:55031231-55031250 Msa1163690:CDS 45.0%
TCCTTCAGCAACAGTCTTCT+TGG - chr7_4:55031235-55031254 None:intergenic 45.0%
CCCCAATTTCGACACAAGTA+GGG - chr7_4:55031290-55031309 None:intergenic 45.0%
CCCTACTTGTGTCGAAATTG+GGG + chr7_4:55031287-55031306 Msa1163690:CDS 45.0%
TCCCCAATTTCGACACAAGT+AGG - chr7_4:55031291-55031310 None:intergenic 45.0%
! TTCCTTTTGCAATCGAGGTG+AGG + chr7_4:55031331-55031350 Msa1163690:intron 45.0%
AACCTCACCTCGATTGCAAA+AGG - chr7_4:55031336-55031355 None:intergenic 45.0%
CCTCTTTGCATGAAATCACG+AGG - chr7_4:55031459-55031478 None:intergenic 45.0%
CCTCGTGATTTCATGCAAAG+AGG + chr7_4:55031456-55031475 Msa1163690:CDS 45.0%
TCATGCAAAGAGGACGAGTT+AGG + chr7_4:55031466-55031485 Msa1163690:CDS 45.0%
CATGCAAAGAGGACGAGTTA+GGG + chr7_4:55031467-55031486 Msa1163690:CDS 45.0%
TGAGTCCAGAGCCATAAAAG+TGG - chr7_4:55031934-55031953 None:intergenic 45.0%
! ACTCAACACTGGATTGGAAC+TGG + chr7_4:55031949-55031968 Msa1163690:intron 45.0%
AAACCCGTTGCATGGTGAAA+AGG + chr7_4:55032873-55032892 Msa1163690:intron 45.0%
AACCCGTTGCATGGTGAAAA+GGG + chr7_4:55032874-55032893 Msa1163690:intron 45.0%
GGCGAAGAATACGTTCTTAG+AGG + chr7_4:55032895-55032914 Msa1163690:intron 45.0%
AGAATTGGGATGAACCTGAG+GGG + chr7_4:55032956-55032975 Msa1163690:intron 45.0%
CATAGATGATCTCTCTGGTG+TGG - chr7_4:55033041-55033060 None:intergenic 45.0%
AATGTTACGCGTTGCAGAGA+TGG + chr7_4:55033269-55033288 Msa1163690:CDS 45.0%
TGGTCCTTCCATGGTGTTTA+GGG - chr7_4:55033296-55033315 None:intergenic 45.0%
TTGGTCCTTCCATGGTGTTT+AGG - chr7_4:55033297-55033316 None:intergenic 45.0%
TCAACAACAACTGCAACTGC+TGG + chr7_4:55033333-55033352 Msa1163690:CDS 45.0%
CTGGACCTTCCAACAAATCT+GGG + chr7_4:55033352-55033371 Msa1163690:CDS 45.0%
!! CCTTTCCCAGATTTGTTGGA+AGG - chr7_4:55033360-55033379 None:intergenic 45.0%
CCTTCCAACAAATCTGGGAA+AGG + chr7_4:55033357-55033376 Msa1163690:CDS 45.0%
TCCAACAAATCTGGGAAAGG+TGG + chr7_4:55033360-55033379 Msa1163690:CDS 45.0%
TCCACCTTTCCCAGATTTGT+TGG - chr7_4:55033364-55033383 None:intergenic 45.0%
AGCAAAACTCATACTCGGAG+AGG - chr7_4:55033442-55033461 None:intergenic 45.0%
GAAGTTGTACCACTCGGAAA+TGG + chr7_4:55033537-55033556 Msa1163690:three_prime_UTR 45.0%
! TTGCCCAAGCCAAGCAATTT+TGG + chr7_4:55033719-55033738 Msa1163690:three_prime_UTR 45.0%
GCAACCAAAATTGCTTGGCT+TGG - chr7_4:55033726-55033745 None:intergenic 45.0%
TTTGGTTGCCCTTAGCAGTA+GGG + chr7_4:55033747-55033766 Msa1163690:three_prime_UTR 45.0%
ACCAACGTCAGCATCAGAGT+CGG - chr7_4:55031741-55031760 None:intergenic 50.0%
ACTCTGATGCTGACGTTGGT+TGG + chr7_4:55031741-55031760 Msa1163690:intron 50.0%
!! TATGGCTCTGGACTCAACAC+TGG + chr7_4:55031938-55031957 Msa1163690:intron 50.0%
CTCTGGACTCAACACTGGAT+TGG + chr7_4:55031943-55031962 Msa1163690:intron 50.0%
GAGAGAGAGAGAGAGAGAGA+AGG + chr7_4:55032842-55032861 Msa1163690:intron 50.0%
GGAAACTGAAACCCGTTGCA+TGG + chr7_4:55032865-55032884 Msa1163690:intron 50.0%
! TCGCCCTTTTCACCATGCAA+CGG - chr7_4:55032880-55032899 None:intergenic 50.0%
GAATTGGGATGAACCTGAGG+GGG + chr7_4:55032957-55032976 Msa1163690:intron 50.0%
GAGATGGTCCCTAAACACCA+TGG + chr7_4:55033285-55033304 Msa1163690:CDS 50.0%
TGGTCCCTAAACACCATGGA+AGG + chr7_4:55033289-55033308 Msa1163690:CDS 50.0%
TGATGCAGTCTCCTGCTTCT+TGG - chr7_4:55033316-55033335 None:intergenic 50.0%
GCTGGACCTTCCAACAAATC+TGG + chr7_4:55033351-55033370 Msa1163690:CDS 50.0%
!! CGGAAATGGGTTTTGCGCAT+AGG + chr7_4:55033551-55033570 Msa1163690:three_prime_UTR 50.0%
CTACTGCTAAGGGCAACCAA+AGG - chr7_4:55033748-55033767 None:intergenic 50.0%
CTTTGGTTGCCCTTAGCAGT+AGG + chr7_4:55033746-55033765 Msa1163690:three_prime_UTR 50.0%
TTGGTCGTCCCTACTGCTAA+GGG - chr7_4:55033758-55033777 None:intergenic 50.0%
! GGTGGCCTCTGGCTTAGAAA+AGG - chr7_4:55031668-55031687 None:intergenic 55.0%
TATGGAAATGGGGTGGCCTC+TGG - chr7_4:55031679-55031698 None:intergenic 55.0%
TCCGACTCTGATGCTGACGT+TGG + chr7_4:55031737-55031756 Msa1163690:intron 55.0%
! ACGTTGGTTGGCGTTTCGCT+TGG + chr7_4:55031753-55031772 Msa1163690:intron 55.0%
! CGCCCTTTTCACCATGCAAC+GGG - chr7_4:55032879-55032898 None:intergenic 55.0%
ATTTCCAGCGGGACGTCTTC+CGG - chr7_4:55032998-55033017 None:intergenic 55.0%
CCTGCTTCTTGGTCCTTCCA+TGG - chr7_4:55033305-55033324 None:intergenic 55.0%
CCATGGAAGGACCAAGAAGC+AGG + chr7_4:55033302-55033321 Msa1163690:CDS 55.0%
GCGCAAAACCCATTTCCGAG+TGG - chr7_4:55033549-55033568 None:intergenic 55.0%
CTTGGTCGTCCCTACTGCTA+AGG - chr7_4:55033759-55033778 None:intergenic 55.0%
CTTAGCAGTAGGGACGACCA+AGG + chr7_4:55033757-55033776 Msa1163690:three_prime_UTR 55.0%
GACGAAGTTGTCTCCCCCTC+AGG - chr7_4:55032973-55032992 None:intergenic 60.0%
GGGGAGACAACTTCGTCCAC+CGG + chr7_4:55032976-55032995 Msa1163690:intron 60.0%
TCCACCGGAAGACGTCCCGC+TGG + chr7_4:55032991-55033010 Msa1163690:intron 70.0%
TCCAGCGGGACGTCTTCCGG+TGG - chr7_4:55032995-55033014 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 55031099 55033863 55031099 ID=Msa1163690;Name=Msa1163690
chr7_4 mRNA 55031099 55033863 55031099 ID=Msa1163690-mRNA-1;Parent=Msa1163690;Name=Msa1163690-mRNA-1;_AED=0.05;_eAED=0.05;_QI=72|1|1|1|1|1|3|465|138
chr7_4 exon 55031099 55031347 55031099 ID=Msa1163690-mRNA-1:exon:16038;Parent=Msa1163690-mRNA-1
chr7_4 exon 55031441 55031540 55031441 ID=Msa1163690-mRNA-1:exon:16039;Parent=Msa1163690-mRNA-1
chr7_4 exon 55033259 55033863 55033259 ID=Msa1163690-mRNA-1:exon:16040;Parent=Msa1163690-mRNA-1
chr7_4 five_prime_UTR 55031099 55031170 55031099 ID=Msa1163690-mRNA-1:five_prime_utr;Parent=Msa1163690-mRNA-1
chr7_4 CDS 55031171 55031347 55031171 ID=Msa1163690-mRNA-1:cds;Parent=Msa1163690-mRNA-1
chr7_4 CDS 55031441 55031540 55031441 ID=Msa1163690-mRNA-1:cds;Parent=Msa1163690-mRNA-1
chr7_4 CDS 55033259 55033398 55033259 ID=Msa1163690-mRNA-1:cds;Parent=Msa1163690-mRNA-1
chr7_4 three_prime_UTR 55033399 55033863 55033399 ID=Msa1163690-mRNA-1:three_prime_utr;Parent=Msa1163690-mRNA-1
Gene Sequence

>Msa1163690

ATGGAAGGTGAATTACCAAGCATAAAGAGATGGATCGTGTTATACCCTGTTTACATCAATTCCAAGAAGACTGTTGCTGAAGGAAGAAGAATCGGAATCTCCAAATCATGCGAAAACCCTACTTGTGTCGAAATTGGGGATTGTTGCAGTTTTCTTAAACTTCCTTTTGCAATCGAGCTTGACAAGGCTTACCCTCGTGATTTCATGCAAAGAGGACGAGTTAGGGTTTTGCTCAAAAAGGAGGATGGGACTCTCATTAATCCATCCATAGCTTCCAGAAAGCAACTAATGTTACGCGTTGCAGAGATGGTCCCTAAACACCATGGAAGGACCAAGAAGCAGGAGACTGCATCAACAACAACTGCAACTGCTGGACCTTCCAACAAATCTGGGAAAGGTGGAAAAAAGAGAAGATAA
Protein sequence

>Msa1163690

MEGELPSIKRWIVLYPVYINSKKTVAEGRRIGISKSCENPTCVEIGDCCSFLKLPFAIELDKAYPRDFMQRGRVRVLLKKEDGTLINPSIASRKQLMLRVAEMVPKHHGRTKKQETASTTTATAGPSNKSGKGGKKRR*