AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1164620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1164620 MtrunA17_Chr7g0266271 100.000 72 0 0 1 72 1 72 6.76e-48 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1164620 AT3G52730.1 80.556 72 14 0 1 72 1 72 7.42e-39 123
Msa1164620 AT3G52730.2 76.786 56 13 0 1 56 1 56 1.07e-26 92.8

Find 39 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTCACTTATTGCAAAA+TGG 0.252781 7_4:+56328006 None:intergenic
TCCGATCTGATTTGAGTTCT+AGG 0.278024 7_4:+56330542 None:intergenic
ATATGATTTATCCCAAATAC+TGG 0.293371 7_4:-56327887 Msa1164620:three_prime_UTR
TCATGTATGGTTTCATTTGC+TGG 0.324683 7_4:-56327962 Msa1164620:three_prime_UTR
AAGAAGAGGAGGAGGCGTTT+TGG 0.335531 7_4:-56330499 Msa1164620:CDS
ATATGATTTATCCCAAATAC+TGG 0.346156 7_4:-56327827 Msa1164620:three_prime_UTR
AATATAATAAAACTTCAAAA+TGG 0.380237 7_4:+56328069 None:intergenic
TCTGGGAAATAAATAATGTT+GGG 0.387527 7_4:-56330282 Msa1164620:CDS
TGTTGACCTTAAGTCATGTA+TGG 0.394843 7_4:-56327975 Msa1164620:three_prime_UTR
TCGTTGCAGGCTGTGGATTA+CGG 0.406296 7_4:-56330316 Msa1164620:intron
ATTACGGTGTTCACAAGCTC+TGG 0.424543 7_4:-56330300 Msa1164620:CDS
TACATTTGAGAACGGTGAAA+AGG 0.431138 7_4:-56330608 Msa1164620:five_prime_UTR
ATGAAACCATACATGACTTA+AGG 0.434265 7_4:+56327969 None:intergenic
CTCTGGGAAATAAATAATGT+TGG 0.443669 7_4:-56330283 Msa1164620:CDS
CCGATCTGATTTGAGTTCTA+GGG 0.456736 7_4:+56330543 None:intergenic
TTCTGTGCTGGGGCAAAGGC+CGG 0.481651 7_4:-56328124 Msa1164620:CDS
TATGTTACCTTCGTCATCGC+CGG 0.481783 7_4:-56330438 Msa1164620:CDS
ATATGAAGACATTTCTGTGC+TGG 0.485619 7_4:-56328136 Msa1164620:CDS
TATGAAGACATTTCTGTGCT+GGG 0.504170 7_4:-56328135 Msa1164620:CDS
TGGTTTCATTTGCTGGCTGT+TGG 0.509038 7_4:-56327955 Msa1164620:three_prime_UTR
ACATTTCTGTGCTGGGGCAA+AGG 0.516213 7_4:-56328128 Msa1164620:CDS
ACATTTGAGAACGGTGAAAA+GGG 0.519176 7_4:-56330607 Msa1164620:five_prime_UTR
CAGACAGTTCACTCGTCTAC+CGG 0.527733 7_4:+56328105 None:intergenic
CCGGTACGGTTCGTTTGAAA+CGG 0.532497 7_4:-56330655 None:intergenic
CTCAAATCAGATCGGAACCA+TGG 0.553505 7_4:-56330535 Msa1164620:exon
ATTGAAGTGAACTTACGAGC+TGG 0.554063 7_4:-56328038 Msa1164620:three_prime_UTR
CCTTTGTTTACATTTGAGAA+CGG 0.567826 7_4:-56330616 Msa1164620:five_prime_UTR
TTACGGTGTTCACAAGCTCT+GGG 0.588806 7_4:-56330299 Msa1164620:CDS
CCCTAGAACTCAAATCAGAT+CGG 0.590863 7_4:-56330543 Msa1164620:five_prime_UTR
CTGGGAAATAAATAATGTTG+GGG 0.603916 7_4:-56330281 Msa1164620:intron
GGCGCATAAGAACCTTGTAA+AGG 0.605705 7_4:+56330472 None:intergenic
CCGTTCTCAAATGTAAACAA+AGG 0.621436 7_4:+56330616 None:intergenic
GCTGCTAAAAGAAGAGGAGG+AGG 0.621931 7_4:-56330507 Msa1164620:CDS
GAATCCGCTGCTAAAAGAAG+AGG 0.626583 7_4:-56330513 Msa1164620:CDS
AAAAGCACCGGCGATGACGA+AGG 0.627325 7_4:+56330431 None:intergenic
TCCGCTGCTAAAAGAAGAGG+AGG 0.631604 7_4:-56330510 Msa1164620:CDS
ATGAAGACATTTCTGTGCTG+GGG 0.643761 7_4:-56328134 Msa1164620:CDS
CCGTTCACCGAGAAAAGCAC+CGG 0.644147 7_4:+56330419 None:intergenic
AGGTAACATAGACAGAGTTG+CGG 0.713031 7_4:+56330451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATAAAACTTCAAAA+TGG + chr7_4:56330351-56330370 None:intergenic 10.0%
!! ATTAAGAGTAAAATTAAAAG+GGG + chr7_4:56328037-56328056 None:intergenic 15.0%
!! GATTAAGAGTAAAATTAAAA+GGG + chr7_4:56328038-56328057 None:intergenic 15.0%
!! TACTATTATATAACTTTGAA+AGG - chr7_4:56328272-56328291 Msa1164620:intron 15.0%
!!! ACTATTATATAACTTTGAAA+GGG - chr7_4:56328273-56328292 Msa1164620:intron 15.0%
!!! TTTTTATAGAATAATCTCTT+AGG - chr7_4:56328367-56328386 Msa1164620:intron 15.0%
!! AATATAATAACAACATAGAT+TGG + chr7_4:56328956-56328975 None:intergenic 15.0%
!!! TTGAAGTTTTATTATATTCA+TGG - chr7_4:56330353-56330372 Msa1164620:intron 15.0%
!! CGATTAAGAGTAAAATTAAA+AGG + chr7_4:56328039-56328058 None:intergenic 20.0%
!!! GCTTATGAAATTTGTATTTT+TGG - chr7_4:56328301-56328320 Msa1164620:intron 20.0%
!! GTTGGAAATATATAGAAAAA+TGG + chr7_4:56329063-56329082 None:intergenic 20.0%
!!! TTGTTTTTACGAAGTAATTA+TGG - chr7_4:56329794-56329813 Msa1164620:intron 20.0%
!! AATTACTTCATATAAACAAC+AGG + chr7_4:56330019-56330038 None:intergenic 20.0%
!! TTGTTTATATGAAGTAATTC+TGG - chr7_4:56330020-56330039 Msa1164620:intron 20.0%
!! CTTGAAAAAAAAGAAGATAA+AGG - chr7_4:56330137-56330156 Msa1164620:intron 20.0%
!!! GTTATCTGAAATTAAGTTAA+AGG - chr7_4:56330168-56330187 Msa1164620:intron 20.0%
!! GTAAAAATATGAATCACAAA+TGG + chr7_4:56330227-56330246 None:intergenic 20.0%
!!! ATTTTCTTAATCCAGTATTT+GGG + chr7_4:56330544-56330563 None:intergenic 20.0%
!!! ATTTTCTTAATCCAGTATTT+GGG + chr7_4:56330544-56330563 None:intergenic 20.0%
! TCTGGGAAATAAATAATGTT+GGG - chr7_4:56328135-56328154 Msa1164620:CDS 25.0%
!! ATATAACTTTGAAAGGGATT+AGG - chr7_4:56328279-56328298 Msa1164620:intron 25.0%
!!! TGTTGAAATGTTTTTTCCTT+GGG - chr7_4:56328344-56328363 Msa1164620:intron 25.0%
! GATTATTCTATAAAAACCCA+AGG + chr7_4:56328363-56328382 None:intergenic 25.0%
! TTAAAGTTATGTCGTGTTAT+TGG - chr7_4:56328538-56328557 Msa1164620:intron 25.0%
!! TGTTATCTCTTTGAAGAATA+TGG - chr7_4:56328569-56328588 Msa1164620:intron 25.0%
! TCTATTCCTATTATTCATTG+TGG - chr7_4:56328659-56328678 Msa1164620:intron 25.0%
! CATCACTATTTCAAAAAAGA+TGG + chr7_4:56329215-56329234 None:intergenic 25.0%
!!! TTTTGAAATAGTGATGTTGT+AGG - chr7_4:56329219-56329238 Msa1164620:intron 25.0%
!! TTTGAAATAGTGATGTTGTA+GGG - chr7_4:56329220-56329239 Msa1164620:intron 25.0%
!! AAAATTGTATTGTGGGATTT+TGG + chr7_4:56329339-56329358 None:intergenic 25.0%
! GAACACAAAAATTGTATTGT+GGG + chr7_4:56329346-56329365 None:intergenic 25.0%
!!! CATTAATCTCTACTTTGTTT+TGG - chr7_4:56329587-56329606 Msa1164620:intron 25.0%
!!! TTTGTTTTGGTTGTTTCTTT+TGG - chr7_4:56329600-56329619 Msa1164620:intron 25.0%
!!! TTTGGTTCTTTTTCACTAAT+CGG - chr7_4:56329618-56329637 Msa1164620:intron 25.0%
! ATATGTTAACATGATCAAGT+AGG + chr7_4:56329699-56329718 None:intergenic 25.0%
! AATTACTTCGTAAAAACAAC+AGG + chr7_4:56329793-56329812 None:intergenic 25.0%
! CATATTCACTTATTGCAAAA+TGG + chr7_4:56330414-56330433 None:intergenic 25.0%
! ATATGATTTATCCCAAATAC+TGG - chr7_4:56330530-56330549 Msa1164620:exon 25.0%
!! GATTTTCTTAATCCAGTATT+TGG + chr7_4:56330545-56330564 None:intergenic 25.0%
! ATATGATTTATCCCAAATAC+TGG - chr7_4:56330530-56330549 Msa1164620:exon 25.0%
!! GATTTTCTTAATCCAGTATT+TGG + chr7_4:56330545-56330564 None:intergenic 25.0%
! CCTTTGTTTACATTTGAGAA+CGG - chr7_4:56327801-56327820 Msa1164620:three_prime_UTR 30.0%
! CTCTGGGAAATAAATAATGT+TGG - chr7_4:56328134-56328153 Msa1164620:CDS 30.0%
CTGGGAAATAAATAATGTTG+GGG - chr7_4:56328136-56328155 Msa1164620:CDS 30.0%
TCGATAGATTAAGCATAAAG+TGG + chr7_4:56328175-56328194 None:intergenic 30.0%
!!! GTGTTGAAATGTTTTTTCCT+TGG - chr7_4:56328343-56328362 Msa1164620:intron 30.0%
! GATTCTGATTTCGTGTTTTA+TGG + chr7_4:56328471-56328490 None:intergenic 30.0%
ATATAACAGACAACTGTGTA+AGG - chr7_4:56328615-56328634 Msa1164620:intron 30.0%
AGGAATCCACAATGAATAAT+AGG + chr7_4:56328668-56328687 None:intergenic 30.0%
TGGATTCCTATTATTCATTG+TGG - chr7_4:56328679-56328698 Msa1164620:intron 30.0%
! TTGTCATCTGATTTTCTCTT+AGG - chr7_4:56328720-56328739 Msa1164620:intron 30.0%
!!! ATTTTCTCTTAGGCTTTGTT+TGG - chr7_4:56328730-56328749 Msa1164620:intron 30.0%
!!! TTTTCTCTTAGGCTTTGTTT+GGG - chr7_4:56328731-56328750 Msa1164620:intron 30.0%
AAGAAATAGAAGACATTGAG+AGG - chr7_4:56328807-56328826 Msa1164620:intron 30.0%
AATAAAAAATCCCCCTCATT+TGG - chr7_4:56328861-56328880 Msa1164620:intron 30.0%
ATAAAAAATCCCCCTCATTT+GGG - chr7_4:56328862-56328881 Msa1164620:intron 30.0%
GGGAACTCAAAAATTGTATT+GGG - chr7_4:56328884-56328903 Msa1164620:intron 30.0%
GGAACTCAAAAATTGTATTG+GGG - chr7_4:56328885-56328904 Msa1164620:intron 30.0%
GAACTCAAAAATTGTATTGG+GGG - chr7_4:56328886-56328905 Msa1164620:intron 30.0%
AAACTTGCAAATAGAACCTA+AGG + chr7_4:56329132-56329151 None:intergenic 30.0%
TTAGGTTCTATTTGCAAGTT+TGG - chr7_4:56329131-56329150 Msa1164620:intron 30.0%
GGAACACAAAAATTGTATTG+TGG + chr7_4:56329347-56329366 None:intergenic 30.0%
AAAACAAAATACCCTCAACT+GGG + chr7_4:56329370-56329389 None:intergenic 30.0%
TAAAACAAAATACCCTCAAC+TGG + chr7_4:56329371-56329390 None:intergenic 30.0%
!!! GTTGAGGGTATTTTGTTTTA+TGG - chr7_4:56329371-56329390 Msa1164620:intron 30.0%
AAAATTGTTTCAACAGGCTT+TGG + chr7_4:56329732-56329751 None:intergenic 30.0%
TCCAGTAAAATTGTTTCAAC+AGG + chr7_4:56329738-56329757 None:intergenic 30.0%
TTTCATTGTTTACGATGCTT+TGG - chr7_4:56329881-56329900 Msa1164620:intron 30.0%
!! ATGACTCTTCTTTTGAAGAA+GGG + chr7_4:56329912-56329931 None:intergenic 30.0%
!! TATGACTCTTCTTTTGAAGA+AGG + chr7_4:56329913-56329932 None:intergenic 30.0%
AAAAGTGACACTAAGCAATT+GGG + chr7_4:56329984-56330003 None:intergenic 30.0%
TTTCATTGTTTACGATGCTT+TGG - chr7_4:56329881-56329900 Msa1164620:intron 30.0%
ATGAAACCATACATGACTTA+AGG + chr7_4:56330451-56330470 None:intergenic 30.0%
CCGTTCTCAAATGTAAACAA+AGG + chr7_4:56327804-56327823 None:intergenic 35.0%
TACATTTGAGAACGGTGAAA+AGG - chr7_4:56327809-56327828 Msa1164620:three_prime_UTR 35.0%
ACATTTGAGAACGGTGAAAA+GGG - chr7_4:56327810-56327829 Msa1164620:three_prime_UTR 35.0%
!!! GTTTCGCTAGTTTTAAGATC+TGG - chr7_4:56328248-56328267 Msa1164620:intron 35.0%
!! GTGTGATTGTTGCTTCATTT+TGG - chr7_4:56328416-56328435 Msa1164620:intron 35.0%
! TCTGATTTCGTGTTTTATGG+TGG + chr7_4:56328468-56328487 None:intergenic 35.0%
GAAACAACAATCACAGTTAC+AGG + chr7_4:56328503-56328522 None:intergenic 35.0%
ATGCAGCCACAATGAATAAT+AGG + chr7_4:56328688-56328707 None:intergenic 35.0%
TTATTCATTGTGGCTGCATT+AGG - chr7_4:56328689-56328708 Msa1164620:intron 35.0%
!!! GAGAGTTTTGGAAAAAAGGA+AGG - chr7_4:56328775-56328794 Msa1164620:intron 35.0%
TAAAAAATCCCCCTCATTTG+GGG - chr7_4:56328863-56328882 Msa1164620:intron 35.0%
GGGGAACTCAAAAATTGTAT+TGG - chr7_4:56328883-56328902 Msa1164620:intron 35.0%
TCAAAAATTGTATTGGGGGA+GGG - chr7_4:56328890-56328909 Msa1164620:intron 35.0%
!! TAGAACCTAAGGCTTTGTTT+GGG + chr7_4:56329121-56329140 None:intergenic 35.0%
!! ATAGAACCTAAGGCTTTGTT+TGG + chr7_4:56329122-56329141 None:intergenic 35.0%
GTTCTATTTGCAAGTTTGGA+GGG - chr7_4:56329135-56329154 Msa1164620:intron 35.0%
TTCTATTTGCAAGTTTGGAG+GGG - chr7_4:56329136-56329155 Msa1164620:intron 35.0%
! TTGTATTGTGGGATTTTGGA+AGG + chr7_4:56329335-56329354 None:intergenic 35.0%
AAACAAAATACCCTCAACTG+GGG + chr7_4:56329369-56329388 None:intergenic 35.0%
! TGAAAGGAATGAAGGACTTT+TGG + chr7_4:56329427-56329446 None:intergenic 35.0%
ATATAAGCTGCAAACACCTA+AGG + chr7_4:56329489-56329508 None:intergenic 35.0%
AGCAGAGCAGGTATATAATT+TGG + chr7_4:56329541-56329560 None:intergenic 35.0%
!! CTTTTTCACTAATCGGTAAC+CGG - chr7_4:56329625-56329644 Msa1164620:intron 35.0%
AGGAAACAATGAGTGAAAGT+CGG + chr7_4:56329668-56329687 None:intergenic 35.0%
! GCCTGTTGAAACAATTTTAC+TGG - chr7_4:56329734-56329753 Msa1164620:intron 35.0%
AAAGTGACACTAAGCAATTG+GGG + chr7_4:56329983-56330002 None:intergenic 35.0%
CAAAAGTGACACTAAGCAAT+TGG + chr7_4:56329985-56330004 None:intergenic 35.0%
ATATGAAGACATTTCTGTGC+TGG - chr7_4:56330281-56330300 Msa1164620:intron 35.0%
TATGAAGACATTTCTGTGCT+GGG - chr7_4:56330282-56330301 Msa1164620:CDS 35.0%
TGTTGACCTTAAGTCATGTA+TGG - chr7_4:56330442-56330461 Msa1164620:CDS 35.0%
TCATGTATGGTTTCATTTGC+TGG - chr7_4:56330455-56330474 Msa1164620:CDS 35.0%
! CCGATCTGATTTGAGTTCTA+GGG + chr7_4:56327877-56327896 None:intergenic 40.0%
CCCTAGAACTCAAATCAGAT+CGG - chr7_4:56327874-56327893 Msa1164620:three_prime_UTR 40.0%
! TCCGATCTGATTTGAGTTCT+AGG + chr7_4:56327878-56327897 None:intergenic 40.0%
AGGTAACATAGACAGAGTTG+CGG + chr7_4:56327969-56327988 None:intergenic 40.0%
!! TTCATTTTGGCATGATACCG+TGG - chr7_4:56328429-56328448 Msa1164620:intron 40.0%
CAGACAACTGTGTAAGGAAA+TGG - chr7_4:56328621-56328640 Msa1164620:intron 40.0%
!! TTAGGCTTTGTTTGGGAGTT+TGG - chr7_4:56328738-56328757 Msa1164620:intron 40.0%
!!! AGGGGAGAGTTTTGGAAAAA+AGG - chr7_4:56328771-56328790 Msa1164620:intron 40.0%
ATAGAAGACATTGAGAGGAG+AGG - chr7_4:56328812-56328831 Msa1164620:intron 40.0%
TAGAAGACATTGAGAGGAGA+GGG - chr7_4:56328813-56328832 Msa1164620:intron 40.0%
AAAAAATCCCCCTCATTTGG+GGG - chr7_4:56328864-56328883 Msa1164620:intron 40.0%
!!! TTTTGAGTTCCCCCAAATGA+GGG + chr7_4:56328876-56328895 None:intergenic 40.0%
!!! TTTTTGAGTTCCCCCAAATG+AGG + chr7_4:56328877-56328896 None:intergenic 40.0%
CTCAAAAATTGTATTGGGGG+AGG - chr7_4:56328889-56328908 Msa1164620:intron 40.0%
! CTTTGTTTGGGAGTTTGAAG+GGG + chr7_4:56329109-56329128 None:intergenic 40.0%
!! GCTTTGTTTGGGAGTTTGAA+GGG + chr7_4:56329110-56329129 None:intergenic 40.0%
AAACTCCCAAACAAAGCCTT+AGG - chr7_4:56329113-56329132 Msa1164620:intron 40.0%
GGTTCTATTTGCAAGTTTGG+AGG - chr7_4:56329134-56329153 Msa1164620:intron 40.0%
TCTATTTGCAAGTTTGGAGG+GGG - chr7_4:56329137-56329156 Msa1164620:intron 40.0%
AACAAAATACCCTCAACTGG+GGG + chr7_4:56329368-56329387 None:intergenic 40.0%
! AGGAATGAAGGACTTTTGGA+GGG + chr7_4:56329423-56329442 None:intergenic 40.0%
! AAGGAATGAAGGACTTTTGG+AGG + chr7_4:56329424-56329443 None:intergenic 40.0%
! GCTTTAGGTGAAAGGAATGA+AGG + chr7_4:56329435-56329454 None:intergenic 40.0%
! ATTTTGTGAGCTGCTGATCT+TGG - chr7_4:56329563-56329582 Msa1164620:intron 40.0%
! ACTAATCGGTAACCGGTTTA+TGG - chr7_4:56329632-56329651 Msa1164620:intron 40.0%
TGATCAAGTAGGATCAGTGT+AGG + chr7_4:56329688-56329707 None:intergenic 40.0%
TTGAATGCAAAAGTGACGCT+AGG + chr7_4:56329766-56329785 None:intergenic 40.0%
ATGAAGACATTTCTGTGCTG+GGG - chr7_4:56330283-56330302 Msa1164620:CDS 40.0%
ATTGAAGTGAACTTACGAGC+TGG - chr7_4:56330379-56330398 Msa1164620:intron 40.0%
CTCAAATCAGATCGGAACCA+TGG - chr7_4:56327882-56327901 Msa1164620:three_prime_UTR 45.0%
!! TCTTTTAGCAGCGGATTCCA+TGG + chr7_4:56327902-56327921 None:intergenic 45.0%
GAATCCGCTGCTAAAAGAAG+AGG - chr7_4:56327904-56327923 Msa1164620:three_prime_UTR 45.0%
! TCCTCCTCTTCTTTTAGCAG+CGG + chr7_4:56327911-56327930 None:intergenic 45.0%
!!! CGTTTTGGAAGGCCTTTACA+AGG - chr7_4:56327933-56327952 Msa1164620:three_prime_UTR 45.0%
GGCGCATAAGAACCTTGTAA+AGG + chr7_4:56327948-56327967 None:intergenic 45.0%
TATGTTACCTTCGTCATCGC+CGG - chr7_4:56327979-56327998 Msa1164620:three_prime_UTR 45.0%
AGATCGCTGAATTTCGTTGC+AGG - chr7_4:56328088-56328107 Msa1164620:three_prime_UTR 45.0%
ATTACGGTGTTCACAAGCTC+TGG - chr7_4:56328117-56328136 Msa1164620:CDS 45.0%
TTACGGTGTTCACAAGCTCT+GGG - chr7_4:56328118-56328137 Msa1164620:CDS 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG - chr7_4:56328742-56328761 Msa1164620:intron 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG - chr7_4:56328743-56328762 Msa1164620:intron 45.0%
GGAAAAAAGGAAGGAGCAAG+TGG - chr7_4:56328784-56328803 Msa1164620:intron 45.0%
! TTTGAGTTCCCCCAAATGAG+GGG + chr7_4:56328875-56328894 None:intergenic 45.0%
! ATTGTATTGGGGGAGGGTTT+TGG - chr7_4:56328896-56328915 Msa1164620:intron 45.0%
! TTGTATTGGGGGAGGGTTTT+GGG - chr7_4:56328897-56328916 Msa1164620:intron 45.0%
TTTGGGAGTTTGAAGGGGAA+GGG + chr7_4:56329104-56329123 None:intergenic 45.0%
!! GGCTTTGTTTGGGAGTTTGA+AGG + chr7_4:56329111-56329130 None:intergenic 45.0%
! TTTTTGTGTTCCCCCAGTTG+AGG - chr7_4:56329355-56329374 Msa1164620:intron 45.0%
! TTTTGTGTTCCCCCAGTTGA+GGG - chr7_4:56329356-56329375 Msa1164620:intron 45.0%
!! GGAATGAAGGACTTTTGGAG+GGG + chr7_4:56329422-56329441 None:intergenic 45.0%
!! AAGAGAGGGCTTTAGGTGAA+AGG + chr7_4:56329443-56329462 None:intergenic 45.0%
TAAGGCTCTGTTTGCGAGTT+TGG + chr7_4:56329471-56329490 None:intergenic 45.0%
AAACTCGCAAACAGAGCCTT+AGG - chr7_4:56329470-56329489 Msa1164620:intron 45.0%
CTCACAAAATTCAGCAGAGC+AGG + chr7_4:56329553-56329572 None:intergenic 45.0%
GGCAGTAGAGAACCATAAAC+CGG + chr7_4:56329647-56329666 None:intergenic 45.0%
! TGGTTTCATTTGCTGGCTGT+TGG - chr7_4:56330462-56330481 Msa1164620:CDS 45.0%
TCCGCTGCTAAAAGAAGAGG+AGG - chr7_4:56327907-56327926 Msa1164620:three_prime_UTR 50.0%
GCTGCTAAAAGAAGAGGAGG+AGG - chr7_4:56327910-56327929 Msa1164620:three_prime_UTR 50.0%
!!! AAGAAGAGGAGGAGGCGTTT+TGG - chr7_4:56327918-56327937 Msa1164620:three_prime_UTR 50.0%
CTGAATTTCGTTGCAGGCTG+TGG - chr7_4:56328094-56328113 Msa1164620:exon 50.0%
! TCGTTGCAGGCTGTGGATTA+CGG - chr7_4:56328101-56328120 Msa1164620:exon 50.0%
!! GGCTTTGTTTGGGAGTTTGG+AGG - chr7_4:56328741-56328760 Msa1164620:intron 50.0%
ACATTGAGAGGAGAGGGCTT+TGG - chr7_4:56328819-56328838 Msa1164620:intron 50.0%
TTGAGTTCCCCCAAATGAGG+GGG + chr7_4:56328874-56328893 None:intergenic 50.0%
! TGTATTGGGGGAGGGTTTTG+GGG - chr7_4:56328898-56328917 Msa1164620:intron 50.0%
GTTTGGGAGTTTGAAGGGGA+AGG + chr7_4:56329105-56329124 None:intergenic 50.0%
ATTTGCAAGTTTGGAGGGGG+AGG - chr7_4:56329140-56329159 Msa1164620:intron 50.0%
TTTGCAAGTTTGGAGGGGGA+GGG - chr7_4:56329141-56329160 Msa1164620:intron 50.0%
!! TTTGGAGGGGATGGCTTTGA+GGG + chr7_4:56329409-56329428 None:intergenic 50.0%
!! TTTTGGAGGGGATGGCTTTG+AGG + chr7_4:56329410-56329429 None:intergenic 50.0%
!! TGAAGGACTTTTGGAGGGGA+TGG + chr7_4:56329418-56329437 None:intergenic 50.0%
CTCTGTTTGCGAGTTTGGAG+GGG + chr7_4:56329466-56329485 None:intergenic 50.0%
GCTCTGTTTGCGAGTTTGGA+GGG + chr7_4:56329467-56329486 None:intergenic 50.0%
ACATTTCTGTGCTGGGGCAA+AGG - chr7_4:56330289-56330308 Msa1164620:CDS 50.0%
CAGACAGTTCACTCGTCTAC+CGG + chr7_4:56330315-56330334 None:intergenic 50.0%
!!! AGAGGAGGAGGCGTTTTGGA+AGG - chr7_4:56327922-56327941 Msa1164620:three_prime_UTR 55.0%
!! AAAAGCACCGGCGATGACGA+AGG + chr7_4:56327989-56328008 None:intergenic 55.0%
! GTCATCGCCGGTGCTTTTCT+CGG - chr7_4:56327991-56328010 Msa1164620:three_prime_UTR 55.0%
! CCGTTCACCGAGAAAAGCAC+CGG + chr7_4:56328001-56328020 None:intergenic 55.0%
! CCGGTGCTTTTCTCGGTGAA+CGG - chr7_4:56327998-56328017 Msa1164620:three_prime_UTR 55.0%
! CGGTGCTTTTCTCGGTGAAC+GGG - chr7_4:56327999-56328018 Msa1164620:three_prime_UTR 55.0%
GATACCGTGGCTGTCAACTG+CGG - chr7_4:56328442-56328461 Msa1164620:intron 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG - chr7_4:56328746-56328765 Msa1164620:intron 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG - chr7_4:56328747-56328766 Msa1164620:intron 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - chr7_4:56328748-56328767 Msa1164620:intron 55.0%
! TTGAGAGGAGAGGGCTTTGG+AGG - chr7_4:56328822-56328841 Msa1164620:intron 55.0%
!! GTATTGGGGGAGGGTTTTGG+GGG - chr7_4:56328899-56328918 Msa1164620:intron 55.0%
!!! TATTGGGGGAGGGTTTTGGG+GGG - chr7_4:56328900-56328919 Msa1164620:intron 55.0%
!!! AGGGTTTTGGGGGGTTATGG+AGG - chr7_4:56328909-56328928 Msa1164620:intron 55.0%
!!! GGGTTTTGGGGGGTTATGGA+GGG - chr7_4:56328910-56328929 Msa1164620:intron 55.0%
GAGTTTGAAGGGGAAGGGAG+GGG + chr7_4:56329099-56329118 None:intergenic 55.0%
GGAGTTTGAAGGGGAAGGGA+GGG + chr7_4:56329100-56329119 None:intergenic 55.0%
!! GGTTTGGGGGTGGATTGTAG+AGG - chr7_4:56329171-56329190 Msa1164620:intron 55.0%
!! TTGGAGGGGATGGCTTTGAG+GGG + chr7_4:56329408-56329427 None:intergenic 55.0%
CGAGTTTGGAGGGGAAGAGA+GGG + chr7_4:56329457-56329476 None:intergenic 55.0%
GGCTCTGTTTGCGAGTTTGG+AGG + chr7_4:56329468-56329487 None:intergenic 55.0%
!!! GGGAGGGTTTTGGGGGGTTA+TGG - chr7_4:56328906-56328925 Msa1164620:intron 60.0%
AAGGGAGGGGAGGGATTTGG+GGG + chr7_4:56329086-56329105 None:intergenic 60.0%
GAAGGGAGGGGAGGGATTTG+GGG + chr7_4:56329087-56329106 None:intergenic 60.0%
GGAAGGGAGGGGAGGGATTT+GGG + chr7_4:56329088-56329107 None:intergenic 60.0%
TTGAAGGGGAAGGGAGGGGA+GGG + chr7_4:56329095-56329114 None:intergenic 60.0%
TTTGAAGGGGAAGGGAGGGG+AGG + chr7_4:56329096-56329115 None:intergenic 60.0%
GGGAGTTTGAAGGGGAAGGG+AGG + chr7_4:56329101-56329120 None:intergenic 60.0%
GGAGGGGAAGAGAGGGCTTT+AGG + chr7_4:56329450-56329469 None:intergenic 60.0%
GCGAGTTTGGAGGGGAAGAG+AGG + chr7_4:56329458-56329477 None:intergenic 60.0%
TTCTGTGCTGGGGCAAAGGC+CGG - chr7_4:56330293-56330312 Msa1164620:CDS 60.0%
GTGGCCGCAGTTGACAGCCA+CGG + chr7_4:56328449-56328468 None:intergenic 65.0%
GGAGTTTGGAGGGGAGGGGA+GGG - chr7_4:56328752-56328771 Msa1164620:intron 65.0%
GAGTTTGGAGGGGAGGGGAG+GGG - chr7_4:56328753-56328772 Msa1164620:intron 65.0%
! GGGAGGGGAGGGGAGAGTTT+TGG - chr7_4:56328763-56328782 Msa1164620:intron 65.0%
AGGGGAGGGATTTGGGGGGT+TGG + chr7_4:56329081-56329100 None:intergenic 65.0%
AGGGAGGGGAGGGATTTGGG+GGG + chr7_4:56329085-56329104 None:intergenic 65.0%
GGGAAGGGAGGGGAGGGATT+TGG + chr7_4:56329089-56329108 None:intergenic 65.0%
GCAAGTTTGGAGGGGGAGGG+AGG - chr7_4:56329144-56329163 Msa1164620:intron 65.0%
GGGAGTTTGGAGGGGAGGGG+AGG - chr7_4:56328751-56328770 Msa1164620:intron 70.0%
TTTGGAGGGGGAGGGAGGCG+AGG - chr7_4:56329149-56329168 Msa1164620:intron 70.0%
TTGGAGGGGGAGGGAGGCGA+GGG - chr7_4:56329150-56329169 Msa1164620:intron 70.0%
GGGGAGGGAGGCGAGGGTTT+GGG - chr7_4:56329156-56329175 Msa1164620:intron 70.0%
GGGAGGGAGGCGAGGGTTTG+GGG - chr7_4:56329157-56329176 Msa1164620:intron 70.0%
GGAGGGAGGCGAGGGTTTGG+GGG - chr7_4:56329158-56329177 Msa1164620:intron 70.0%
GGGGGAGGGAGGCGAGGGTT+TGG - chr7_4:56329155-56329174 Msa1164620:intron 75.0%
GGGAGGCGAGGGTTTGGGGG+TGG - chr7_4:56329161-56329180 Msa1164620:intron 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 56327770 56330669 56327770 ID=Msa1164620;Name=Msa1164620
chr7_4 mRNA 56327770 56330669 56327770 ID=Msa1164620-mRNA-1;Parent=Msa1164620;Name=Msa1164620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=131|1|1|1|1|1|3|343|72
chr7_4 exon 56327770 56328163 56327770 ID=Msa1164620-mRNA-1:exon:16795;Parent=Msa1164620-mRNA-1
chr7_4 exon 56330282 56330329 56330282 ID=Msa1164620-mRNA-1:exon:16794;Parent=Msa1164620-mRNA-1
chr7_4 exon 56330419 56330669 56330419 ID=Msa1164620-mRNA-1:exon:16793;Parent=Msa1164620-mRNA-1
chr7_4 five_prime_UTR 56330539 56330669 56330539 ID=Msa1164620-mRNA-1:five_prime_utr;Parent=Msa1164620-mRNA-1
chr7_4 CDS 56330419 56330538 56330419 ID=Msa1164620-mRNA-1:cds;Parent=Msa1164620-mRNA-1
chr7_4 CDS 56330282 56330329 56330282 ID=Msa1164620-mRNA-1:cds;Parent=Msa1164620-mRNA-1
chr7_4 CDS 56328113 56328163 56328113 ID=Msa1164620-mRNA-1:cds;Parent=Msa1164620-mRNA-1
chr7_4 three_prime_UTR 56327770 56328112 56327770 ID=Msa1164620-mRNA-1:three_prime_utr;Parent=Msa1164620-mRNA-1
Gene Sequence

>Msa1164620

ATGGAATCCGCTGCTAAAAGAAGAGGAGGAGGCGTTTTGGAAGGCCTTTACAAGGTTCTTATGCGCCGCAACTCTGTCTATGTTACCTTCGTCATCGCCGGTGCTTTTCTCGGTGAACGGGCTGTGGATTACGGTGTTCACAAGCTCTGGGAAATAAATAATGTTGGGAAACGATATGAAGACATTTCTGTGCTGGGGCAAAGGCCGGTAGACGAGTGA
Protein sequence

>Msa1164620

MESAAKRRGGGVLEGLYKVLMRRNSVYVTFVIAGAFLGERAVDYGVHKLWEINNVGKRYEDISVLGQRPVDE*