AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1386200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386200 MtrunA17_Chr7g0266271 100.000 72 0 0 1 72 1 72 6.76e-48 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1386200 AT3G52730.1 80.556 72 14 0 1 72 1 72 7.42e-39 123
Msa1386200 AT3G52730.2 76.786 56 13 0 1 56 1 56 1.07e-26 92.8

Find 52 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTCACTTATTGCAAAA+TGG 0.252781 tig0020787:-95925 None:intergenic
TCCGATCTGATTTGAGTTCT+AGG 0.278024 tig0020787:-93423 None:intergenic
ATATGATTTATCCCAAATAC+TGG 0.293371 tig0020787:+96044 Msa1386200:three_prime_UTR
TCATGTATGGTTTCATTTGC+TGG 0.324683 tig0020787:+95969 Msa1386200:three_prime_UTR
AAGAAGAGGAGGAGGCGTTT+TGG 0.335531 tig0020787:+93466 Msa1386200:CDS
TTAATTAAATTAAATTAAAT+CGG 0.340843 tig0020787:-96124 None:intergenic
AATATAATAAAACTTCAAAA+TGG 0.380237 tig0020787:-95862 None:intergenic
CAGCTGCTTTGAATTTCAGC+AGG 0.381477 tig0020787:-96193 None:intergenic
TCTGGGAAATAAATAATGTT+GGG 0.387527 tig0020787:+93676 Msa1386200:CDS
TGTTGACCTTAAGTCATGTA+TGG 0.394843 tig0020787:+95956 Msa1386200:three_prime_UTR
TCGTTGCAGGCTGTGGATTA+CGG 0.406296 tig0020787:+93642 Msa1386200:intron
TCAATAGCGAATGCGGAAAC+AGG 0.413736 tig0020787:+93269 None:intergenic
ATTACGGTGTTCACAAGCTC+TGG 0.424543 tig0020787:+93658 Msa1386200:CDS
TACATTTGAGAACGGTGAAA+AGG 0.431138 tig0020787:+93359 Msa1386200:five_prime_UTR
ATGAAACCATACATGACTTA+AGG 0.434265 tig0020787:-95962 None:intergenic
CTCTGGGAAATAAATAATGT+TGG 0.443669 tig0020787:+93675 Msa1386200:CDS
CCGATCTGATTTGAGTTCTA+GGG 0.456736 tig0020787:-93422 None:intergenic
TTCTGTGCTGGGGCAAAGGC+CGG 0.481651 tig0020787:+95807 Msa1386200:CDS
TATGTTACCTTCGTCATCGC+CGG 0.481783 tig0020787:+93527 Msa1386200:CDS
ATATGAAGACATTTCTGTGC+TGG 0.485619 tig0020787:+95795 Msa1386200:CDS
ATGCGGAAACAGGCAGTGTC+CGG 0.495979 tig0020787:+93279 Msa1386200:five_prime_UTR
TATGAAGACATTTCTGTGCT+GGG 0.504170 tig0020787:+95796 Msa1386200:CDS
ATTAAATTAAATTAAATCGG+TGG 0.504656 tig0020787:-96121 None:intergenic
TGGTTTCATTTGCTGGCTGT+TGG 0.509038 tig0020787:+95976 Msa1386200:three_prime_UTR
ACATTTCTGTGCTGGGGCAA+AGG 0.516213 tig0020787:+95803 Msa1386200:CDS
ACATTTGAGAACGGTGAAAA+GGG 0.519176 tig0020787:+93360 Msa1386200:five_prime_UTR
CAGACAGTTCACTCGTCTAC+CGG 0.527733 tig0020787:-95826 None:intergenic
TTAAATTAAATTAAATCGGT+GGG 0.527868 tig0020787:-96120 None:intergenic
CCGGTACGGTTCGTTTGAAA+CGG 0.532497 tig0020787:+93312 Msa1386200:five_prime_UTR
CCGTTTCAAACGAACCGTAC+CGG 0.537500 tig0020787:-93312 None:intergenic
CTCAAATCAGATCGGAACCA+TGG 0.553505 tig0020787:+93430 Msa1386200:exon
ATTGAAGTGAACTTACGAGC+TGG 0.554063 tig0020787:+95893 Msa1386200:three_prime_UTR
CCGGTCGGATCGAGCCGGTA+CGG 0.558201 tig0020787:+93298 Msa1386200:five_prime_UTR
CCTTTGTTTACATTTGAGAA+CGG 0.567826 tig0020787:+93351 Msa1386200:five_prime_UTR
AGCTGCTTTGAATTTCAGCA+GGG 0.588712 tig0020787:-96192 None:intergenic
TTACGGTGTTCACAAGCTCT+GGG 0.588806 tig0020787:+93659 Msa1386200:CDS
CCGTACCGGCTCGATCCGAC+CGG 0.589872 tig0020787:-93298 None:intergenic
CCCTAGAACTCAAATCAGAT+CGG 0.590863 tig0020787:+93422 Msa1386200:five_prime_UTR
AGTGTCCGGTCGGATCGAGC+CGG 0.597440 tig0020787:+93293 Msa1386200:five_prime_UTR
CTGGGAAATAAATAATGTTG+GGG 0.603916 tig0020787:+93677 Msa1386200:CDS
GGCGCATAAGAACCTTGTAA+AGG 0.605705 tig0020787:-93493 None:intergenic
CAGCCCATCAATAGCGAATG+CGG 0.615932 tig0020787:+93262 None:intergenic
CCGTTCTCAAATGTAAACAA+AGG 0.621436 tig0020787:-93351 None:intergenic
GCTGCTAAAAGAAGAGGAGG+AGG 0.621931 tig0020787:+93458 Msa1386200:CDS
GAATCCGCTGCTAAAAGAAG+AGG 0.626583 tig0020787:+93452 Msa1386200:CDS
AAAAGCACCGGCGATGACGA+AGG 0.627325 tig0020787:-93534 None:intergenic
TCCGCTGCTAAAAGAAGAGG+AGG 0.631604 tig0020787:+93455 Msa1386200:CDS
ATGAAGACATTTCTGTGCTG+GGG 0.643761 tig0020787:+95797 Msa1386200:CDS
CCGTTCACCGAGAAAAGCAC+CGG 0.644147 tig0020787:-93546 None:intergenic
GGAAACAGGCAGTGTCCGGT+CGG 0.659778 tig0020787:+93283 Msa1386200:five_prime_UTR
GCTGCTTTGAATTTCAGCAG+GGG 0.684683 tig0020787:-96191 None:intergenic
AGGTAACATAGACAGAGTTG+CGG 0.713031 tig0020787:-93514 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAAATTAAATTAAAT+CGG - tig0020787:96127-96146 None:intergenic 0.0%
!! AATATAATAAAACTTCAAAA+TGG - tig0020787:95865-95884 None:intergenic 10.0%
!! ATTAAGAGTAAAATTAAAAG+GGG - tig0020787:93585-93604 None:intergenic 15.0%
!! GATTAAGAGTAAAATTAAAA+GGG - tig0020787:93586-93605 None:intergenic 15.0%
!! TACTATTATATAACTTTGAA+AGG + tig0020787:93813-93832 Msa1386200:intron 15.0%
!!! ACTATTATATAACTTTGAAA+GGG + tig0020787:93814-93833 Msa1386200:intron 15.0%
!!! TTTTTATAGAATAATCTCTT+AGG + tig0020787:93908-93927 Msa1386200:intron 15.0%
!! AATATAATAACAACATAGAT+TGG - tig0020787:94478-94497 None:intergenic 15.0%
!!! TTGAAGTTTTATTATATTCA+TGG + tig0020787:95867-95886 Msa1386200:three_prime_UTR 15.0%
!! TTAAATTAAATTAAATCGGT+GGG - tig0020787:96123-96142 None:intergenic 15.0%
!! ATTAAATTAAATTAAATCGG+TGG - tig0020787:96124-96143 None:intergenic 15.0%
!! CGATTAAGAGTAAAATTAAA+AGG - tig0020787:93587-93606 None:intergenic 20.0%
!!! GCTTATGAAATTTGTATTTT+TGG + tig0020787:93842-93861 Msa1386200:intron 20.0%
!! GTTGGAAATATATAGAAAAA+TGG - tig0020787:94585-94604 None:intergenic 20.0%
!! AATTACTTCATATAAACAAC+AGG - tig0020787:95533-95552 None:intergenic 20.0%
!! TTGTTTATATGAAGTAATTC+TGG + tig0020787:95534-95553 Msa1386200:intron 20.0%
!! CTTGAAAAAAAAGAAGATAA+AGG + tig0020787:95651-95670 Msa1386200:intron 20.0%
!!! GTTATCTGAAATTAAGTTAA+AGG + tig0020787:95682-95701 Msa1386200:intron 20.0%
!! GTAAAAATATGAATCACAAA+TGG - tig0020787:95741-95760 None:intergenic 20.0%
!!! ATTTTCTTAATCCAGTATTT+GGG - tig0020787:96058-96077 None:intergenic 20.0%
! TCTGGGAAATAAATAATGTT+GGG + tig0020787:93676-93695 Msa1386200:CDS 25.0%
!! ATATAACTTTGAAAGGGATT+AGG + tig0020787:93820-93839 Msa1386200:intron 25.0%
!!! TGTTGAAATGTTTTTTCCTT+GGG + tig0020787:93885-93904 Msa1386200:intron 25.0%
! GATTATTCTATAAAAACCCA+AGG - tig0020787:93904-93923 None:intergenic 25.0%
!! TGTTATCTCTTTGAAGAATA+TGG + tig0020787:94111-94130 Msa1386200:intron 25.0%
! TCTATTCCTATTATTCATTG+TGG + tig0020787:94201-94220 Msa1386200:intron 25.0%
!!! ATTTTCTCTTATGCTTTGTT+TGG + tig0020787:94252-94271 Msa1386200:intron 25.0%
!!! TTTTCTCTTATGCTTTGTTT+GGG + tig0020787:94253-94272 Msa1386200:intron 25.0%
! CATCACTATTTCAAAAAAGA+TGG - tig0020787:94737-94756 None:intergenic 25.0%
!!! TTTTGAAATAGTGATGTTGT+AGG + tig0020787:94741-94760 Msa1386200:intron 25.0%
!! TTTGAAATAGTGATGTTGTA+GGG + tig0020787:94742-94761 Msa1386200:intron 25.0%
!! AAAATTGTATTGTGGGATTT+TGG - tig0020787:94861-94880 None:intergenic 25.0%
! GAACACAAAAATTGTATTGT+GGG - tig0020787:94868-94887 None:intergenic 25.0%
!!! CATTAATCTCTACTTTGTTT+TGG + tig0020787:95109-95128 Msa1386200:intron 25.0%
!!! TTTGTTTTGGTTGTTTCTTT+TGG + tig0020787:95122-95141 Msa1386200:intron 25.0%
!!! TTTGGTTCTTTTTCACTAAT+CGG + tig0020787:95140-95159 Msa1386200:intron 25.0%
! AATTACTTCGTAAAAACAAC+AGG - tig0020787:95307-95326 None:intergenic 25.0%
!!! TTGTTTTTACGAAGTAATTC+TGG + tig0020787:95308-95327 Msa1386200:intron 25.0%
! CATATTCACTTATTGCAAAA+TGG - tig0020787:95928-95947 None:intergenic 25.0%
! ATATGATTTATCCCAAATAC+TGG + tig0020787:96044-96063 Msa1386200:three_prime_UTR 25.0%
!! GATTTTCTTAATCCAGTATT+TGG - tig0020787:96059-96078 None:intergenic 25.0%
! CCTTTGTTTACATTTGAGAA+CGG + tig0020787:93351-93370 Msa1386200:five_prime_UTR 30.0%
! CTCTGGGAAATAAATAATGT+TGG + tig0020787:93675-93694 Msa1386200:CDS 30.0%
CTGGGAAATAAATAATGTTG+GGG + tig0020787:93677-93696 Msa1386200:CDS 30.0%
TCGATAGATTAAGCATAAAG+TGG - tig0020787:93716-93735 None:intergenic 30.0%
!!! GTGTTGAAATGTTTTTTCCT+TGG + tig0020787:93884-93903 Msa1386200:intron 30.0%
! GATTCTGATTTCGTGTTTTA+TGG - tig0020787:94012-94031 None:intergenic 30.0%
TAAGTTATGTCGTGTGTTAT+TGG + tig0020787:94080-94099 Msa1386200:intron 30.0%
ATATAACAGACAACTGTGTA+AGG + tig0020787:94157-94176 Msa1386200:intron 30.0%
TGGAAGAAATAGAAGACATT+GGG + tig0020787:94326-94345 Msa1386200:intron 30.0%
AATAAAAAATCCCCCTCATT+TGG + tig0020787:94383-94402 Msa1386200:intron 30.0%
ATAAAAAATCCCCCTCATTT+GGG + tig0020787:94384-94403 Msa1386200:intron 30.0%
GGGAACTCAAAAATTGTATT+GGG + tig0020787:94406-94425 Msa1386200:intron 30.0%
GGAACTCAAAAATTGTATTG+GGG + tig0020787:94407-94426 Msa1386200:intron 30.0%
GAACTCAAAAATTGTATTGG+GGG + tig0020787:94408-94427 Msa1386200:intron 30.0%
AAACTTGCAAATAGAACCTA+AGG - tig0020787:94654-94673 None:intergenic 30.0%
TTAGGTTCTATTTGCAAGTT+TGG + tig0020787:94653-94672 Msa1386200:intron 30.0%
GGAACACAAAAATTGTATTG+TGG - tig0020787:94869-94888 None:intergenic 30.0%
AAAACAAAATACCCTCAACT+GGG - tig0020787:94892-94911 None:intergenic 30.0%
TAAAACAAAATACCCTCAAC+TGG - tig0020787:94893-94912 None:intergenic 30.0%
!!! GTTGAGGGTATTTTGTTTTA+TGG + tig0020787:94893-94912 Msa1386200:intron 30.0%
TGTTAACATGATCAAGTAGT+AGG - tig0020787:95210-95229 None:intergenic 30.0%
AAAATTGTTTCAACAGGCTT+TGG - tig0020787:95246-95265 None:intergenic 30.0%
TCCAGTAAAATTGTTTCAAC+AGG - tig0020787:95252-95271 None:intergenic 30.0%
TTTCATTGTTTACGATGCTT+TGG + tig0020787:95395-95414 Msa1386200:intron 30.0%
!! ATGACTCTTCTTTTGAAGAA+GGG - tig0020787:95426-95445 None:intergenic 30.0%
!! TATGACTCTTCTTTTGAAGA+AGG - tig0020787:95427-95446 None:intergenic 30.0%
AAAAGTGACACTAAGCAATT+GGG - tig0020787:95498-95517 None:intergenic 30.0%
TTTCATTGTTTACGATGCTT+TGG + tig0020787:95395-95414 Msa1386200:intron 30.0%
ATGAAACCATACATGACTTA+AGG - tig0020787:95965-95984 None:intergenic 30.0%
CCGTTCTCAAATGTAAACAA+AGG - tig0020787:93354-93373 None:intergenic 35.0%
TACATTTGAGAACGGTGAAA+AGG + tig0020787:93359-93378 Msa1386200:five_prime_UTR 35.0%
ACATTTGAGAACGGTGAAAA+GGG + tig0020787:93360-93379 Msa1386200:five_prime_UTR 35.0%
!!! GTTTCGCTAGTTTTAAGATC+TGG + tig0020787:93789-93808 Msa1386200:intron 35.0%
!! GTGTGATTGTTGCTTCATTT+TGG + tig0020787:93957-93976 Msa1386200:intron 35.0%
! TCTGATTTCGTGTTTTATGG+TGG - tig0020787:94009-94028 None:intergenic 35.0%
GAAACAACAATCACAGTTAC+AGG - tig0020787:94044-94063 None:intergenic 35.0%
ATGCAGCCACAATGAATAAT+AGG - tig0020787:94210-94229 None:intergenic 35.0%
TTATTCATTGTGGCTGCATT+AGG + tig0020787:94211-94230 Msa1386200:intron 35.0%
!! TTATGCTTTGTTTGGGAGTT+TGG + tig0020787:94260-94279 Msa1386200:intron 35.0%
!!! GAGAGTTTTGGAAAAAAGGA+AGG + tig0020787:94297-94316 Msa1386200:intron 35.0%
GTGGAAGAAATAGAAGACAT+TGG + tig0020787:94325-94344 Msa1386200:intron 35.0%
AAGAAATAGAAGACATTGGG+AGG + tig0020787:94329-94348 Msa1386200:intron 35.0%
TAAAAAATCCCCCTCATTTG+GGG + tig0020787:94385-94404 Msa1386200:intron 35.0%
GGGGAACTCAAAAATTGTAT+TGG + tig0020787:94405-94424 Msa1386200:intron 35.0%
TCAAAAATTGTATTGGGGGA+GGG + tig0020787:94412-94431 Msa1386200:intron 35.0%
!! TAGAACCTAAGGCTTTGTTT+GGG - tig0020787:94643-94662 None:intergenic 35.0%
!! ATAGAACCTAAGGCTTTGTT+TGG - tig0020787:94644-94663 None:intergenic 35.0%
GTTCTATTTGCAAGTTTGGA+GGG + tig0020787:94657-94676 Msa1386200:intron 35.0%
TTCTATTTGCAAGTTTGGAG+GGG + tig0020787:94658-94677 Msa1386200:intron 35.0%
! TTGTATTGTGGGATTTTGGA+AGG - tig0020787:94857-94876 None:intergenic 35.0%
AAACAAAATACCCTCAACTG+GGG - tig0020787:94891-94910 None:intergenic 35.0%
! TGAAAGGAATGAAGGACTTT+TGG - tig0020787:94949-94968 None:intergenic 35.0%
ATATAAGCTGCAAACACCTA+AGG - tig0020787:95011-95030 None:intergenic 35.0%
AGCAGAGCAGGTATATAATT+TGG - tig0020787:95063-95082 None:intergenic 35.0%
!! CTTTTTCACTAATCGGTAAC+CGG + tig0020787:95147-95166 Msa1386200:intron 35.0%
AGGAAACAATGAGTGAAAGT+CGG - tig0020787:95190-95209 None:intergenic 35.0%
! GCCTGTTGAAACAATTTTAC+TGG + tig0020787:95248-95267 Msa1386200:intron 35.0%
AAAGTGACACTAAGCAATTG+GGG - tig0020787:95497-95516 None:intergenic 35.0%
CAAAAGTGACACTAAGCAAT+TGG - tig0020787:95499-95518 None:intergenic 35.0%
ATATGAAGACATTTCTGTGC+TGG + tig0020787:95795-95814 Msa1386200:CDS 35.0%
TATGAAGACATTTCTGTGCT+GGG + tig0020787:95796-95815 Msa1386200:CDS 35.0%
TGTTGACCTTAAGTCATGTA+TGG + tig0020787:95956-95975 Msa1386200:three_prime_UTR 35.0%
TCATGTATGGTTTCATTTGC+TGG + tig0020787:95969-95988 Msa1386200:three_prime_UTR 35.0%
! CCGATCTGATTTGAGTTCTA+GGG - tig0020787:93425-93444 None:intergenic 40.0%
CCCTAGAACTCAAATCAGAT+CGG + tig0020787:93422-93441 Msa1386200:five_prime_UTR 40.0%
! TCCGATCTGATTTGAGTTCT+AGG - tig0020787:93426-93445 None:intergenic 40.0%
AGGTAACATAGACAGAGTTG+CGG - tig0020787:93517-93536 None:intergenic 40.0%
!! TTCATTTTGGCATGATACCG+TGG + tig0020787:93970-93989 Msa1386200:intron 40.0%
CAGACAACTGTGTAAGGAAA+TGG + tig0020787:94163-94182 Msa1386200:intron 40.0%
!!! AGGGGAGAGTTTTGGAAAAA+AGG + tig0020787:94293-94312 Msa1386200:intron 40.0%
AAAAAATCCCCCTCATTTGG+GGG + tig0020787:94386-94405 Msa1386200:intron 40.0%
!!! TTTTGAGTTCCCCCAAATGA+GGG - tig0020787:94398-94417 None:intergenic 40.0%
!!! TTTTTGAGTTCCCCCAAATG+AGG - tig0020787:94399-94418 None:intergenic 40.0%
CTCAAAAATTGTATTGGGGG+AGG + tig0020787:94411-94430 Msa1386200:intron 40.0%
! CTTTGTTTGGGAGTTTGAAG+GGG - tig0020787:94631-94650 None:intergenic 40.0%
!! GCTTTGTTTGGGAGTTTGAA+GGG - tig0020787:94632-94651 None:intergenic 40.0%
AAACTCCCAAACAAAGCCTT+AGG + tig0020787:94635-94654 Msa1386200:intron 40.0%
GGTTCTATTTGCAAGTTTGG+AGG + tig0020787:94656-94675 Msa1386200:intron 40.0%
TCTATTTGCAAGTTTGGAGG+GGG + tig0020787:94659-94678 Msa1386200:intron 40.0%
AACAAAATACCCTCAACTGG+GGG - tig0020787:94890-94909 None:intergenic 40.0%
! AGGAATGAAGGACTTTTGGA+GGG - tig0020787:94945-94964 None:intergenic 40.0%
! AAGGAATGAAGGACTTTTGG+AGG - tig0020787:94946-94965 None:intergenic 40.0%
! GCTTTAGGTGAAAGGAATGA+AGG - tig0020787:94957-94976 None:intergenic 40.0%
! ATTTTGTGAGCTGCTGATCT+TGG + tig0020787:95085-95104 Msa1386200:intron 40.0%
! ACTAATCGGTAACCGGTTTA+TGG + tig0020787:95154-95173 Msa1386200:intron 40.0%
TTGAATGCAAAAGTGACGCT+AGG - tig0020787:95280-95299 None:intergenic 40.0%
ATGAAGACATTTCTGTGCTG+GGG + tig0020787:95797-95816 Msa1386200:CDS 40.0%
ATTGAAGTGAACTTACGAGC+TGG + tig0020787:95893-95912 Msa1386200:three_prime_UTR 40.0%
! AGCTGCTTTGAATTTCAGCA+GGG - tig0020787:96195-96214 None:intergenic 40.0%
CTCAAATCAGATCGGAACCA+TGG + tig0020787:93430-93449 Msa1386200:exon 45.0%
!! TCTTTTAGCAGCGGATTCCA+TGG - tig0020787:93450-93469 None:intergenic 45.0%
GAATCCGCTGCTAAAAGAAG+AGG + tig0020787:93452-93471 Msa1386200:CDS 45.0%
! TCCTCCTCTTCTTTTAGCAG+CGG - tig0020787:93459-93478 None:intergenic 45.0%
!!! CGTTTTGGAAGGCCTTTACA+AGG + tig0020787:93481-93500 Msa1386200:CDS 45.0%
GGCGCATAAGAACCTTGTAA+AGG - tig0020787:93496-93515 None:intergenic 45.0%
TATGTTACCTTCGTCATCGC+CGG + tig0020787:93527-93546 Msa1386200:CDS 45.0%
AGATCGCTGAATTTCGTTGC+AGG + tig0020787:93629-93648 Msa1386200:intron 45.0%
ATTACGGTGTTCACAAGCTC+TGG + tig0020787:93658-93677 Msa1386200:CDS 45.0%
TTACGGTGTTCACAAGCTCT+GGG + tig0020787:93659-93678 Msa1386200:CDS 45.0%
!! TGCTTTGTTTGGGAGTTTGG+AGG + tig0020787:94263-94282 Msa1386200:intron 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG + tig0020787:94264-94283 Msa1386200:intron 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG + tig0020787:94265-94284 Msa1386200:intron 45.0%
GGAAAAAAGGAAGGAGCAAG+TGG + tig0020787:94306-94325 Msa1386200:intron 45.0%
ATAGAAGACATTGGGAGGAG+AGG + tig0020787:94334-94353 Msa1386200:intron 45.0%
TAGAAGACATTGGGAGGAGA+GGG + tig0020787:94335-94354 Msa1386200:intron 45.0%
! TTTGAGTTCCCCCAAATGAG+GGG - tig0020787:94397-94416 None:intergenic 45.0%
! ATTGTATTGGGGGAGGGTTT+TGG + tig0020787:94418-94437 Msa1386200:intron 45.0%
! TTGTATTGGGGGAGGGTTTT+GGG + tig0020787:94419-94438 Msa1386200:intron 45.0%
TTTGGGAGTTTGAAGGGGAA+GGG - tig0020787:94626-94645 None:intergenic 45.0%
!! GGCTTTGTTTGGGAGTTTGA+AGG - tig0020787:94633-94652 None:intergenic 45.0%
! TTTTTGTGTTCCCCCAGTTG+AGG + tig0020787:94877-94896 Msa1386200:intron 45.0%
! TTTTGTGTTCCCCCAGTTGA+GGG + tig0020787:94878-94897 Msa1386200:intron 45.0%
!! GGAATGAAGGACTTTTGGAG+GGG - tig0020787:94944-94963 None:intergenic 45.0%
!! AAGAGAGGGCTTTAGGTGAA+AGG - tig0020787:94965-94984 None:intergenic 45.0%
TAAGGCTCTGTTTGCGAGTT+TGG - tig0020787:94993-95012 None:intergenic 45.0%
AAACTCGCAAACAGAGCCTT+AGG + tig0020787:94992-95011 Msa1386200:intron 45.0%
CTCACAAAATTCAGCAGAGC+AGG - tig0020787:95075-95094 None:intergenic 45.0%
GGCAGTAGAGAACCATAAAC+CGG - tig0020787:95169-95188 None:intergenic 45.0%
! TGGTTTCATTTGCTGGCTGT+TGG + tig0020787:95976-95995 Msa1386200:three_prime_UTR 45.0%
! GCTGCTTTGAATTTCAGCAG+GGG - tig0020787:96194-96213 None:intergenic 45.0%
! CAGCTGCTTTGAATTTCAGC+AGG - tig0020787:96196-96215 None:intergenic 45.0%
CCGTTTCAAACGAACCGTAC+CGG - tig0020787:93315-93334 None:intergenic 50.0%
CCGGTACGGTTCGTTTGAAA+CGG + tig0020787:93312-93331 Msa1386200:five_prime_UTR 50.0%
TCCGCTGCTAAAAGAAGAGG+AGG + tig0020787:93455-93474 Msa1386200:CDS 50.0%
GCTGCTAAAAGAAGAGGAGG+AGG + tig0020787:93458-93477 Msa1386200:CDS 50.0%
!!! AAGAAGAGGAGGAGGCGTTT+TGG + tig0020787:93466-93485 Msa1386200:CDS 50.0%
CTGAATTTCGTTGCAGGCTG+TGG + tig0020787:93635-93654 Msa1386200:intron 50.0%
! TCGTTGCAGGCTGTGGATTA+CGG + tig0020787:93642-93661 Msa1386200:intron 50.0%
TTGAGTTCCCCCAAATGAGG+GGG - tig0020787:94396-94415 None:intergenic 50.0%
! TGTATTGGGGGAGGGTTTTG+GGG + tig0020787:94420-94439 Msa1386200:intron 50.0%
GTTTGGGAGTTTGAAGGGGA+AGG - tig0020787:94627-94646 None:intergenic 50.0%
ATTTGCAAGTTTGGAGGGGG+AGG + tig0020787:94662-94681 Msa1386200:intron 50.0%
TTTGCAAGTTTGGAGGGGGA+GGG + tig0020787:94663-94682 Msa1386200:intron 50.0%
!! TTTGGAGGGGATGGCTTTGA+GGG - tig0020787:94931-94950 None:intergenic 50.0%
!! TTTTGGAGGGGATGGCTTTG+AGG - tig0020787:94932-94951 None:intergenic 50.0%
!! TGAAGGACTTTTGGAGGGGA+TGG - tig0020787:94940-94959 None:intergenic 50.0%
CTCTGTTTGCGAGTTTGGAG+GGG - tig0020787:94988-95007 None:intergenic 50.0%
GCTCTGTTTGCGAGTTTGGA+GGG - tig0020787:94989-95008 None:intergenic 50.0%
ACATTTCTGTGCTGGGGCAA+AGG + tig0020787:95803-95822 Msa1386200:CDS 50.0%
CAGACAGTTCACTCGTCTAC+CGG - tig0020787:95829-95848 None:intergenic 50.0%
ATGCGGAAACAGGCAGTGTC+CGG + tig0020787:93279-93298 Msa1386200:five_prime_UTR 55.0%
!!! AGAGGAGGAGGCGTTTTGGA+AGG + tig0020787:93470-93489 Msa1386200:CDS 55.0%
!! AAAAGCACCGGCGATGACGA+AGG - tig0020787:93537-93556 None:intergenic 55.0%
! GTCATCGCCGGTGCTTTTCT+CGG + tig0020787:93539-93558 Msa1386200:CDS 55.0%
! CCGTTCACCGAGAAAAGCAC+CGG - tig0020787:93549-93568 None:intergenic 55.0%
! CCGGTGCTTTTCTCGGTGAA+CGG + tig0020787:93546-93565 Msa1386200:CDS 55.0%
! CGGTGCTTTTCTCGGTGAAC+GGG + tig0020787:93547-93566 Msa1386200:CDS 55.0%
GATACCGTGGCTGTCAACTG+CGG + tig0020787:93983-94002 Msa1386200:intron 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG + tig0020787:94268-94287 Msa1386200:intron 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG + tig0020787:94269-94288 Msa1386200:intron 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG + tig0020787:94270-94289 Msa1386200:intron 55.0%
ACATTGGGAGGAGAGGGCTT+TGG + tig0020787:94341-94360 Msa1386200:intron 55.0%
!! GTATTGGGGGAGGGTTTTGG+GGG + tig0020787:94421-94440 Msa1386200:intron 55.0%
!!! TATTGGGGGAGGGTTTTGGG+GGG + tig0020787:94422-94441 Msa1386200:intron 55.0%
!!! AGGGTTTTGGGGGGTTATGG+AGG + tig0020787:94431-94450 Msa1386200:intron 55.0%
!!! GGGTTTTGGGGGGTTATGGA+GGG + tig0020787:94432-94451 Msa1386200:intron 55.0%
GAGTTTGAAGGGGAAGGGAG+GGG - tig0020787:94621-94640 None:intergenic 55.0%
GGAGTTTGAAGGGGAAGGGA+GGG - tig0020787:94622-94641 None:intergenic 55.0%
!! GGTTTGGGGGTGGATTGTAG+AGG + tig0020787:94693-94712 Msa1386200:intron 55.0%
!! TTGGAGGGGATGGCTTTGAG+GGG - tig0020787:94930-94949 None:intergenic 55.0%
CGAGTTTGGAGGGGAAGAGA+GGG - tig0020787:94979-94998 None:intergenic 55.0%
GGCTCTGTTTGCGAGTTTGG+AGG - tig0020787:94990-95009 None:intergenic 55.0%
GGAAACAGGCAGTGTCCGGT+CGG + tig0020787:93283-93302 Msa1386200:five_prime_UTR 60.0%
! TTGGGAGGAGAGGGCTTTGG+AGG + tig0020787:94344-94363 Msa1386200:intron 60.0%
!!! GGGAGGGTTTTGGGGGGTTA+TGG + tig0020787:94428-94447 Msa1386200:intron 60.0%
AAGGGAGGGGAGGGATTTGG+GGG - tig0020787:94608-94627 None:intergenic 60.0%
GAAGGGAGGGGAGGGATTTG+GGG - tig0020787:94609-94628 None:intergenic 60.0%
GGAAGGGAGGGGAGGGATTT+GGG - tig0020787:94610-94629 None:intergenic 60.0%
TTGAAGGGGAAGGGAGGGGA+GGG - tig0020787:94617-94636 None:intergenic 60.0%
TTTGAAGGGGAAGGGAGGGG+AGG - tig0020787:94618-94637 None:intergenic 60.0%
GGGAGTTTGAAGGGGAAGGG+AGG - tig0020787:94623-94642 None:intergenic 60.0%
GGAGGGGAAGAGAGGGCTTT+AGG - tig0020787:94972-94991 None:intergenic 60.0%
GCGAGTTTGGAGGGGAAGAG+AGG - tig0020787:94980-94999 None:intergenic 60.0%
TTCTGTGCTGGGGCAAAGGC+CGG + tig0020787:95807-95826 Msa1386200:CDS 60.0%
AGTGTCCGGTCGGATCGAGC+CGG + tig0020787:93293-93312 Msa1386200:five_prime_UTR 65.0%
GTGGCCGCAGTTGACAGCCA+CGG - tig0020787:93990-94009 None:intergenic 65.0%
GGAGTTTGGAGGGGAGGGGA+GGG + tig0020787:94274-94293 Msa1386200:intron 65.0%
GAGTTTGGAGGGGAGGGGAG+GGG + tig0020787:94275-94294 Msa1386200:intron 65.0%
! GGGAGGGGAGGGGAGAGTTT+TGG + tig0020787:94285-94304 Msa1386200:intron 65.0%
AGGGGAGGGATTTGGGGGGT+TGG - tig0020787:94603-94622 None:intergenic 65.0%
AGGGAGGGGAGGGATTTGGG+GGG - tig0020787:94607-94626 None:intergenic 65.0%
GGGAAGGGAGGGGAGGGATT+TGG - tig0020787:94611-94630 None:intergenic 65.0%
GCAAGTTTGGAGGGGGAGGG+AGG + tig0020787:94666-94685 Msa1386200:intron 65.0%
!! CCGTACCGGCTCGATCCGAC+CGG - tig0020787:93301-93320 None:intergenic 70.0%
CCGGTCGGATCGAGCCGGTA+CGG + tig0020787:93298-93317 Msa1386200:five_prime_UTR 70.0%
GGGAGTTTGGAGGGGAGGGG+AGG + tig0020787:94273-94292 Msa1386200:intron 70.0%
TTTGGAGGGGGAGGGAGGCG+AGG + tig0020787:94671-94690 Msa1386200:intron 70.0%
TTGGAGGGGGAGGGAGGCGA+GGG + tig0020787:94672-94691 Msa1386200:intron 70.0%
GGGGAGGGAGGCGAGGGTTT+GGG + tig0020787:94678-94697 Msa1386200:intron 70.0%
GGGAGGGAGGCGAGGGTTTG+GGG + tig0020787:94679-94698 Msa1386200:intron 70.0%
GGAGGGAGGCGAGGGTTTGG+GGG + tig0020787:94680-94699 Msa1386200:intron 70.0%
GGGGGAGGGAGGCGAGGGTT+TGG + tig0020787:94677-94696 Msa1386200:intron 75.0%
GGGAGGCGAGGGTTTGGGGG+TGG + tig0020787:94683-94702 Msa1386200:intron 75.0%
Chromosome Type Strat End Strand Name
tig0020787 gene 93274 96229 93274 ID=Msa1386200;Name=Msa1386200
tig0020787 mRNA 93274 96229 93274 ID=Msa1386200-mRNA-1;Parent=Msa1386200;Name=Msa1386200-mRNA-1;_AED=0.09;_eAED=0.09;_QI=175|1|1|1|1|1|3|389|72
tig0020787 exon 93274 93568 93274 ID=Msa1386200-mRNA-1:exon:10010;Parent=Msa1386200-mRNA-1
tig0020787 exon 93651 93698 93651 ID=Msa1386200-mRNA-1:exon:10011;Parent=Msa1386200-mRNA-1
tig0020787 exon 95790 96229 95790 ID=Msa1386200-mRNA-1:exon:10012;Parent=Msa1386200-mRNA-1
tig0020787 five_prime_UTR 93274 93448 93274 ID=Msa1386200-mRNA-1:five_prime_utr;Parent=Msa1386200-mRNA-1
tig0020787 CDS 93449 93568 93449 ID=Msa1386200-mRNA-1:cds;Parent=Msa1386200-mRNA-1
tig0020787 CDS 93651 93698 93651 ID=Msa1386200-mRNA-1:cds;Parent=Msa1386200-mRNA-1
tig0020787 CDS 95790 95840 95790 ID=Msa1386200-mRNA-1:cds;Parent=Msa1386200-mRNA-1
tig0020787 three_prime_UTR 95841 96229 95841 ID=Msa1386200-mRNA-1:three_prime_utr;Parent=Msa1386200-mRNA-1
Gene Sequence

>Msa1386200

ATGGAATCCGCTGCTAAAAGAAGAGGAGGAGGCGTTTTGGAAGGCCTTTACAAGGTTCTTATGCGCCGCAACTCTGTCTATGTTACCTTCGTCATCGCCGGTGCTTTTCTCGGTGAACGGGCTGTGGATTACGGTGTTCACAAGCTCTGGGAAATAAATAATGTTGGGAAACGATATGAAGACATTTCTGTGCTGGGGCAAAGGCCGGTAGACGAGTGA
Protein sequence

>Msa1386200

MESAAKRRGGGVLEGLYKVLMRRNSVYVTFVIAGAFLGERAVDYGVHKLWEINNVGKRYEDISVLGQRPVDE*