AlfalfaGEDB | Msa0604960

M. sativa cultivar zhongmu-4 / Msa0604960

KEGG: K02923
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015934 GO:0016043 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022625 GO:0022626 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0065003 GO:0071704 GO:0071826 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01781.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604960	XP_003606815.2	100.000	68	0	0	12	79	2	69	2.78e-40	137

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604960	sp\|O22860\|RL38_ARATH	92.647	68	5	0	12	79	2	69	2.00e-40	130

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0045730	Msa0604960	0.829064	6.343929e-55	-8.615850e-47
Msa0049690	Msa0604960	0.805229	1.476667e-49	-8.615850e-47
Msa0069440	Msa0604960	0.843000	1.833634e-58	-8.615850e-47
Msa0089120	Msa0604960	0.818767	1.650950e-52	-8.615850e-47
Msa0116130	Msa0604960	0.837033	6.600185e-57	-8.615850e-47
Msa0604960	Msa0623200	0.825331	4.969082e-54	-8.615850e-47
Msa0604960	Msa0625570	0.802211	6.247297e-49	-8.615850e-47
Msa0604960	Msa0633570	0.800712	1.267052e-48	-8.615850e-47
Msa0604960	Msa0659030	0.860605	1.838959e-63	-8.615850e-47
Msa0604960	Msa0660590	0.804018	2.641415e-49	-8.615850e-47
Msa0604960	Msa0683660	0.807372	5.219866e-50	-8.615850e-47
Msa0604960	Msa0683780	0.813607	2.350031e-51	-8.615850e-47
Msa0604960	Msa0705000	0.857389	1.687403e-62	-8.615850e-47
Msa0604960	Msa0705040	0.865916	4.173526e-65	-8.615850e-47
Msa0604960	Msa0705800	0.851213	1.027549e-60	-8.615850e-47
Msa0604960	Msa0706500	0.802749	4.840085e-49	-8.615850e-47
Msa0604960	Msa0712610	0.893708	4.886267e-75	-8.615850e-47
Msa0604960	Msa0729200	0.832055	1.175693e-55	-8.615850e-47
Msa0604960	Msa0752910	0.860540	1.923257e-63	-8.615850e-47
Msa0604960	Msa0766080	0.831703	1.436643e-55	-8.615850e-47
Msa0604960	Msa0769590	0.821718	3.479507e-53	-8.615850e-47
Msa0604960	Msa0781080	0.851439	8.866229e-61	-8.615850e-47
Msa0604960	Msa0783630	0.805152	1.532379e-49	-8.615850e-47
Msa0604960	Msa0785130	0.822653	2.110892e-53	-8.615850e-47
Msa0604960	Msa0792420	0.850033	2.205856e-60	-8.615850e-47
Msa0604960	Msa0822580	0.845475	3.969215e-59	-8.615850e-47
Msa0604960	Msa0827380	0.811440	6.992509e-51	-8.615850e-47
Msa0604960	Msa0876170	0.801992	6.930436e-49	-8.615850e-47
Msa0604960	Msa0888810	0.813860	2.066950e-51	-8.615850e-47
Msa0604960	Msa0915540	0.832167	1.103013e-55	-8.615850e-47
Msa0604960	Msa0945480	0.883936	2.941073e-71	-8.615850e-47
Msa0604960	Msa1021120	0.840578	7.992174e-58	-8.615850e-47
Msa0604960	Msa1035120	0.811093	8.317592e-51	-8.615850e-47
Msa0604960	Msa1037290	0.830099	3.553084e-55	-8.615850e-47
Msa0604960	Msa1080350	0.839614	1.425753e-57	-8.615850e-47
Msa0604960	Msa1082170	0.820015	8.574969e-53	-8.615850e-47
Msa0604960	Msa1085470	0.847293	1.267432e-59	-8.615850e-47
Msa0604960	Msa1115590	0.869453	3.061333e-66	-8.615850e-47
Msa0604960	Msa1125500	0.880055	7.522687e-70	-8.615850e-47
Msa0604960	Msa1125580	0.899625	1.646476e-77	-8.615850e-47
Msa0604960	Msa1125860	0.831263	1.843207e-55	-8.615850e-47
Msa0604960	Msa1126060	0.828291	9.756762e-55	-8.615850e-47
Msa0604960	Msa1126520	0.865163	7.209270e-65	-8.615850e-47
Msa0604960	Msa1164150	0.811409	7.103477e-51	-8.615850e-47
Msa0604960	Msa1164620	0.804701	1.903772e-49	-8.615850e-47
Msa0604960	Msa1166870	0.816358	5.761673e-52	-8.615850e-47
Msa0604960	Msa1184370	0.814567	1.442908e-51	-8.615850e-47
Msa0604960	Msa1193690	0.838059	3.600609e-57	-8.615850e-47
Msa0604960	Msa1218170	0.812128	4.954169e-51	-8.615850e-47
Msa0604960	Msa1227690	0.836458	9.247219e-57	-8.615850e-47
Msa0604960	Msa1241190	0.832811	7.639489e-56	-8.615850e-47
Msa0604960	Msa1256840	0.805830	1.104754e-49	-8.615850e-47
Msa0604960	Msa1278070	0.802042	6.767138e-49	-8.615850e-47
Msa0604960	Msa1316680	0.832788	7.741509e-56	-8.615850e-47
Msa0604960	Msa1321790	0.829680	4.495702e-55	-8.615850e-47
Msa0604960	Msa1345450	0.802120	6.521994e-49	-8.615850e-47
Msa0604960	Msa1349570	0.847767	9.389493e-60	-8.615850e-47
Msa0604960	Msa1356790	0.878814	2.071059e-69	-8.615850e-47
Msa0604960	Msa1362790	0.863329	2.691115e-64	-8.615850e-47
Msa0604960	Msa1368920	0.828065	1.105683e-54	-8.615850e-47
Msa0604960	Msa1369190	0.875178	3.781930e-68	-8.615850e-47
Msa0604960	Msa1374240	0.896463	3.602955e-76	-8.615850e-47
Msa0604960	Msa1386200	0.814585	1.429270e-51	-8.615850e-47
Msa0604960	Msa1392590	0.839365	1.655146e-57	-8.615850e-47
Msa0604960	Msa1403910	0.825114	5.591087e-54	-8.615850e-47
Msa0604960	Msa1406080	0.824662	7.147859e-54	-8.615850e-47
Msa0604960	Msa1413260	0.803648	3.153179e-49	-8.615850e-47
Msa0604960	Msa1425670	0.811814	5.798286e-51	-8.615850e-47
Msa0604960	Msa1426490	0.836554	8.742616e-57	-8.615850e-47
Msa0604960	Msa1428810	0.822938	1.812138e-53	-8.615850e-47
Msa0604960	Msa1437280	0.806662	7.377476e-50	-8.615850e-47
Msa0604960	Msa1442850	0.816618	5.039018e-52	-8.615850e-47
Msa0604960	Msa1450280	0.807748	4.344464e-50	-8.615850e-47
Msa0604960	Msa1455950	0.840239	9.802661e-58	-8.615850e-47
Msa0604960	Msa1456050	0.807505	4.892801e-50	-8.615850e-47
Msa0604960	Msa1462860	0.878357	2.999348e-69	-8.615850e-47
Msa0123010	Msa0604960	0.864486	1.174479e-64	-8.615850e-47
Msa0131560	Msa0604960	0.834151	3.537766e-56	-8.615850e-47
Msa0138890	Msa0604960	0.828720	7.684145e-55	-8.615850e-47
Msa0143230	Msa0604960	0.837917	3.917200e-57	-8.615850e-47
Msa0174770	Msa0604960	0.809347	1.978996e-50	-8.615850e-47
Msa0177360	Msa0604960	0.843301	1.524724e-58	-8.615850e-47
Msa0181610	Msa0604960	0.809997	1.434905e-50	-8.615850e-47
Msa0197980	Msa0604960	0.810778	9.734518e-51	-8.615850e-47
Msa0219120	Msa0604960	0.829825	4.145124e-55	-8.615850e-47
Msa0221030	Msa0604960	0.824559	7.559620e-54	-8.615850e-47
Msa0224560	Msa0604960	0.854181	1.460046e-61	-8.615850e-47
Msa0376820	Msa0604960	0.804191	2.431871e-49	-8.615850e-47
Msa0443390	Msa0604960	0.801576	8.434902e-49	-8.615850e-47
Msa0472130	Msa0604960	0.806457	8.151348e-50	-8.615850e-47
Msa0481850	Msa0604960	0.851311	9.639508e-61	-8.615850e-47
Msa0297830	Msa0604960	0.807506	4.889791e-50	-8.615850e-47
Msa0298200	Msa0604960	0.803751	3.001328e-49	-8.615850e-47
Msa0333910	Msa0604960	0.811539	6.657180e-51	-8.615850e-47
Msa0335840	Msa0604960	0.812226	4.715636e-51	-8.615850e-47
Msa0337670	Msa0604960	0.800130	1.664628e-48	-8.615850e-47
Msa0348830	Msa0604960	0.833948	3.976590e-56	-8.615850e-47
Msa0359600	Msa0604960	0.812199	4.781741e-51	-8.615850e-47
Msa0534750	Msa0604960	0.836117	1.129225e-56	-8.615850e-47
Msa0573350	Msa0604960	0.819590	1.072080e-52	-8.615850e-47
Msa0584030	Msa0604960	0.835873	1.302487e-56	-8.615850e-47
Msa0590940	Msa0604960	0.844326	8.102253e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604960	MtrunA17_Chr4g0033761	100.000	68	0	12	79	2	69	2.56e-44	137
Msa0604960	MtrunA17_Chr2g0285451	94.118	68	4	12	79	2	69	2.88e-41	129
Msa0604960	MtrunA17_Chr8g0376751	95.522	67	3	13	79	3	69	3.51e-41	129
Msa0604960	MtrunA17_Chr7g0216191	91.176	68	6	12	79	2	69	1.54e-37	120

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604960	AT2G43460.1	92.647	68	5	0	12	79	2	69	2.04e-41	130
Msa0604960	AT3G59540.1	92.647	68	5	0	12	79	2	69	2.04e-41	130

Find 21 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTATATTGAAGTCTTTC+CGG	0.185075	4_2:-50727392	Msa0604960:three_prime_UTR
CTAAGTCAATAGAGGTTAAT+TGG	0.262257	4_2:-50727345	Msa0604960:three_prime_UTR
TAAGCAAATCAATGAGATTA+AGG	0.290766	4_2:-50728265	Msa0604960:CDS
TTTGCTTCTCTTGATCTTCA+CGG	0.377542	4_2:+50728206	None:intergenic
CAAAGATGTTGTCAAGTTCA+AGG	0.412153	4_2:-50728187	Msa0604960:CDS
GTCGTACATTTGTTTCCAAA+AGG	0.441235	4_2:-50728957	Msa0604960:CDS
AGACTTCAATATAAGATAAG+AGG	0.449457	4_2:+50727398	None:intergenic
TCACTCAGTACACATTTAAC+CGG	0.452174	4_2:+50727575	None:intergenic
GTGAAGATCAAGTGTTGCTT+TGG	0.469681	4_2:-50727556	Msa0604960:three_prime_UTR
GCAAGACTTGTAATTCTATC+CGG	0.485897	4_2:-50727594	Msa0604960:exon
ACAAAGTGTGTACAAGTACT+TGG	0.506752	4_2:+50728155	None:intergenic
TTAATCTCATTGATTTGCTT+AGG	0.531275	4_2:+50728267	None:intergenic
CTTACGACTCTCATTATGAA+TGG	0.536568	4_2:-50727480	Msa0604960:three_prime_UTR
GTCAATAGAGGTTAATTGGA+TGG	0.539702	4_2:-50727341	Msa0604960:three_prime_UTR
TACTGACAAGAAAATAGAGT+TGG	0.557694	4_2:+50727456	None:intergenic
AGGACTTCCTCCTCACTGCT+AGG	0.557805	4_2:-50728245	Msa0604960:CDS
TCATAATGAGAGTCGTAAGC+TGG	0.559002	4_2:+50727484	None:intergenic
CCTCCTCACTGCTAGGAGGA+AGG	0.566472	4_2:-50728238	Msa0604960:CDS
CCTTCCTCCTAGCAGTGAGG+AGG	0.657731	4_2:+50728238	None:intergenic
ACTTCCTCCTCACTGCTAGG+AGG	0.666318	4_2:-50728242	Msa0604960:CDS
CATCCTTCCTCCTAGCAGTG+AGG	0.669946	4_2:+50728235	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTATATTTTTTTTTATT+TGG	-	chr4_2:50727893-50727912	Msa0604960:intron	0.0%
!!!	TTATTATATTTTTTTTTATT+TGG	-	chr4_2:50727893-50727912	Msa0604960:intron	0.0%
!!!	AATTTTTTTTACTCAATTTT+AGG	-	chr4_2:50727743-50727762	Msa0604960:intron	10.0%
!!!	ATTTTTTTTACTCAATTTTA+GGG	-	chr4_2:50727744-50727763	Msa0604960:intron	10.0%
!!!	TATTTTTTTTTATTTGGATT+TGG	-	chr4_2:50727899-50727918	Msa0604960:intron	10.0%
!!	AATCTATAAAGTAAATAATA+AGG	-	chr4_2:50728341-50728360	Msa0604960:intron	10.0%
!!	ATCTATAAAGTAAATAATAA+GGG	-	chr4_2:50728342-50728361	Msa0604960:intron	10.0%
!!!	AATTTTTTTTACTCAATTTT+AGG	-	chr4_2:50727743-50727762	Msa0604960:intron	10.0%
!!!	ATTTTTTTTACTCAATTTTA+GGG	-	chr4_2:50727744-50727763	Msa0604960:intron	10.0%
!!!	TATTTTTTTTTATTTGGATT+TGG	-	chr4_2:50727899-50727918	Msa0604960:intron	10.0%
!!	AATCTATAAAGTAAATAATA+AGG	-	chr4_2:50728341-50728360	Msa0604960:intron	10.0%
!!	ATCTATAAAGTAAATAATAA+GGG	-	chr4_2:50728342-50728361	Msa0604960:intron	10.0%
!!	ATTGAGTAAAAAAAATTCAT+AGG	+	chr4_2:50727740-50727759	None:intergenic	15.0%
!!	GTGTAATAATTGTTTAAAAA+TGG	-	chr4_2:50728318-50728337	Msa0604960:intron	15.0%
!!	TCTATAAAGTAAATAATAAG+GGG	-	chr4_2:50728343-50728362	Msa0604960:intron	15.0%
!!!	TGTTATTTGTTATTTATTCT+AGG	-	chr4_2:50728546-50728565	Msa0604960:intron	15.0%
!!	ATTGAGTAAAAAAAATTCAT+AGG	+	chr4_2:50727740-50727759	None:intergenic	15.0%
!!	GTGTAATAATTGTTTAAAAA+TGG	-	chr4_2:50728318-50728337	Msa0604960:intron	15.0%
!!	TCTATAAAGTAAATAATAAG+GGG	-	chr4_2:50728343-50728362	Msa0604960:intron	15.0%
!!!	TGTTATTTGTTATTTATTCT+AGG	-	chr4_2:50728546-50728565	Msa0604960:intron	15.0%
!!!	TTTTCATACATTTTTCCTTT+TGG	+	chr4_2:50727314-50727333	None:intergenic	20.0%
!!!	TCAAATTTGTCTATCTTTTA+AGG	+	chr4_2:50727341-50727360	None:intergenic	20.0%
!!!	TCAATTCACAATTTTTTTGT+AGG	-	chr4_2:50727630-50727649	Msa0604960:intron	20.0%
!!!	ACAATTTTTTTGTAGGAAAT+TGG	-	chr4_2:50727637-50727656	Msa0604960:intron	20.0%
!!	AAAACATTGAAAATCAAGAT+AGG	+	chr4_2:50727680-50727699	None:intergenic	20.0%
!!	ATTATACATTGATCTGAATT+AGG	-	chr4_2:50727805-50727824	Msa0604960:intron	20.0%
!!	TTATACATTGATCTGAATTA+GGG	-	chr4_2:50727806-50727825	Msa0604960:intron	20.0%
!!	ACATTTCAATAAACACAAAA+AGG	+	chr4_2:50727964-50727983	None:intergenic	20.0%
!!	AAACAATTATTACACCTTTA+AGG	+	chr4_2:50728313-50728332	None:intergenic	20.0%
!!	TGAGATTTATGAATAAGAAA+TGG	+	chr4_2:50728416-50728435	None:intergenic	20.0%
!!	TCAGATTTATGAATATGAAA+TGG	+	chr4_2:50728511-50728530	None:intergenic	20.0%
!!	ACTACAAGACAAAAATATAT+TGG	+	chr4_2:50728735-50728754	None:intergenic	20.0%
!!	AAACATATTCAAAAAACAAC+CGG	+	chr4_2:50728883-50728902	None:intergenic	20.0%
!!!	TTTTCATACATTTTTCCTTT+TGG	+	chr4_2:50727314-50727333	None:intergenic	20.0%
!!!	TCAAATTTGTCTATCTTTTA+AGG	+	chr4_2:50727341-50727360	None:intergenic	20.0%
!!!	TCAATTCACAATTTTTTTGT+AGG	-	chr4_2:50727630-50727649	Msa0604960:intron	20.0%
!!!	ACAATTTTTTTGTAGGAAAT+TGG	-	chr4_2:50727637-50727656	Msa0604960:intron	20.0%
!!	AAAACATTGAAAATCAAGAT+AGG	+	chr4_2:50727680-50727699	None:intergenic	20.0%
!!	ATTATACATTGATCTGAATT+AGG	-	chr4_2:50727805-50727824	Msa0604960:intron	20.0%
!!	TTATACATTGATCTGAATTA+GGG	-	chr4_2:50727806-50727825	Msa0604960:intron	20.0%
!!	ACATTTCAATAAACACAAAA+AGG	+	chr4_2:50727964-50727983	None:intergenic	20.0%
!!	AAACAATTATTACACCTTTA+AGG	+	chr4_2:50728313-50728332	None:intergenic	20.0%
!!	TGAGATTTATGAATAAGAAA+TGG	+	chr4_2:50728416-50728435	None:intergenic	20.0%
!!	TCAGATTTATGAATATGAAA+TGG	+	chr4_2:50728511-50728530	None:intergenic	20.0%
!!	ACTACAAGACAAAAATATAT+TGG	+	chr4_2:50728735-50728754	None:intergenic	20.0%
!!	AAACATATTCAAAAAACAAC+CGG	+	chr4_2:50728883-50728902	None:intergenic	20.0%
!!	TACCGTCTTTTCATAATATA+GGG	-	chr4_2:50727458-50727477	Msa0604960:three_prime_UTR	25.0%
!	AACCCTATATTATGAAAAGA+CGG	+	chr4_2:50727463-50727482	None:intergenic	25.0%
!	AACAAGTGAAATTGAATCAA+TGG	-	chr4_2:50727774-50727793	Msa0604960:intron	25.0%
!!!	TGGTTTTTGATTTTCAAACT+TGG	-	chr4_2:50727835-50727854	Msa0604960:intron	25.0%
!!!	TTAATCTCATTGATTTGCTT+AGG	+	chr4_2:50727989-50728008	None:intergenic	25.0%
!	TAAGCAAATCAATGAGATTA+AGG	-	chr4_2:50727988-50728007	Msa0604960:intron	25.0%
!	TAATTCTCAAAAACTAACCT+GGG	+	chr4_2:50728168-50728187	None:intergenic	25.0%
!	ATAATTCTCAAAAACTAACC+TGG	+	chr4_2:50728169-50728188	None:intergenic	25.0%
!!!	TAGTTTTTGAGAATTATCGT+TGG	-	chr4_2:50728172-50728191	Msa0604960:CDS	25.0%
!	CAACAGAAATAAACAATCTA+TGG	+	chr4_2:50728273-50728292	None:intergenic	25.0%
!!!	CATAGATTGTTTATTTCTGT+TGG	-	chr4_2:50728271-50728290	Msa0604960:intron	25.0%
!!!	TTATTTCTGTTGGTTGTTAT+AGG	-	chr4_2:50728281-50728300	Msa0604960:intron	25.0%
!	TCTTATTCATAAATCTCAAC+TGG	-	chr4_2:50728418-50728437	Msa0604960:intron	25.0%
!	CTTATTCATAAATCTCAACT+GGG	-	chr4_2:50728419-50728438	Msa0604960:intron	25.0%
!	TTATGAATATGAAATGGAAG+AGG	+	chr4_2:50728505-50728524	None:intergenic	25.0%
!!	AGATGTAGAAATTTGAGTTT+AGG	-	chr4_2:50728592-50728611	Msa0604960:intron	25.0%
!	AGACTTCAATATAAGATAAG+AGG	+	chr4_2:50728858-50728877	None:intergenic	25.0%
!	ATCTTATATTGAAGTCTTTC+CGG	-	chr4_2:50728861-50728880	Msa0604960:intron	25.0%
!!	TCAATCAGTAGAAAGTTTTT+AGG	-	chr4_2:50728951-50728970	Msa0604960:CDS	25.0%
!!	TACCGTCTTTTCATAATATA+GGG	-	chr4_2:50727458-50727477	Msa0604960:three_prime_UTR	25.0%
!	AACCCTATATTATGAAAAGA+CGG	+	chr4_2:50727463-50727482	None:intergenic	25.0%
!	AACAAGTGAAATTGAATCAA+TGG	-	chr4_2:50727774-50727793	Msa0604960:intron	25.0%
!!!	TGGTTTTTGATTTTCAAACT+TGG	-	chr4_2:50727835-50727854	Msa0604960:intron	25.0%
!!!	TTAATCTCATTGATTTGCTT+AGG	+	chr4_2:50727989-50728008	None:intergenic	25.0%
!	TAAGCAAATCAATGAGATTA+AGG	-	chr4_2:50727988-50728007	Msa0604960:intron	25.0%
!	TAATTCTCAAAAACTAACCT+GGG	+	chr4_2:50728168-50728187	None:intergenic	25.0%
!	ATAATTCTCAAAAACTAACC+TGG	+	chr4_2:50728169-50728188	None:intergenic	25.0%
!!!	TAGTTTTTGAGAATTATCGT+TGG	-	chr4_2:50728172-50728191	Msa0604960:CDS	25.0%
!	CAACAGAAATAAACAATCTA+TGG	+	chr4_2:50728273-50728292	None:intergenic	25.0%
!!!	CATAGATTGTTTATTTCTGT+TGG	-	chr4_2:50728271-50728290	Msa0604960:intron	25.0%
!!!	TTATTTCTGTTGGTTGTTAT+AGG	-	chr4_2:50728281-50728300	Msa0604960:intron	25.0%
!	TCTTATTCATAAATCTCAAC+TGG	-	chr4_2:50728418-50728437	Msa0604960:intron	25.0%
!	CTTATTCATAAATCTCAACT+GGG	-	chr4_2:50728419-50728438	Msa0604960:intron	25.0%
!	TTATGAATATGAAATGGAAG+AGG	+	chr4_2:50728505-50728524	None:intergenic	25.0%
!!	AGATGTAGAAATTTGAGTTT+AGG	-	chr4_2:50728592-50728611	Msa0604960:intron	25.0%
!	AGACTTCAATATAAGATAAG+AGG	+	chr4_2:50728858-50728877	None:intergenic	25.0%
!	ATCTTATATTGAAGTCTTTC+CGG	-	chr4_2:50728861-50728880	Msa0604960:intron	25.0%
!!	TCAATCAGTAGAAAGTTTTT+AGG	-	chr4_2:50728951-50728970	Msa0604960:CDS	25.0%
	TTTGATGCATTAGCAAACAA+TGG	-	chr4_2:50727356-50727375	Msa0604960:three_prime_UTR	30.0%
	TTGATGCATTAGCAAACAAT+GGG	-	chr4_2:50727357-50727376	Msa0604960:three_prime_UTR	30.0%
	CACAAACTAAACTCTTAAAC+CGG	-	chr4_2:50727394-50727413	Msa0604960:three_prime_UTR	30.0%
!	GTACCGTCTTTTCATAATAT+AGG	-	chr4_2:50727457-50727476	Msa0604960:three_prime_UTR	30.0%
!	TCGAAGGTTTGAGAATTTTA+GGG	+	chr4_2:50727502-50727521	None:intergenic	30.0%
	GATCTGAATTAGGGAATTTA+TGG	-	chr4_2:50727815-50727834	Msa0604960:intron	30.0%
!!	TCAAACTTGGTAGTTGATAA+AGG	-	chr4_2:50727848-50727867	Msa0604960:intron	30.0%
!!	CAAACTTGGTAGTTGATAAA+GGG	-	chr4_2:50727849-50727868	Msa0604960:intron	30.0%
	AATTCTCAAAAACTAACCTG+GGG	+	chr4_2:50728167-50728186	None:intergenic	30.0%
!	TTATCGTTGGTTGTATGTTA+TGG	-	chr4_2:50728185-50728204	Msa0604960:CDS	30.0%
	ATAATAAGGGGATTGTGTAA+TGG	-	chr4_2:50728355-50728374	Msa0604960:intron	30.0%
	TGTAATGGCCAAATAGATAT+GGG	-	chr4_2:50728370-50728389	Msa0604960:intron	30.0%
	ATATTCTCCCCATATCTATT+TGG	+	chr4_2:50728381-50728400	None:intergenic	30.0%
	TTATGAATAAGAAATGGCAG+AGG	+	chr4_2:50728410-50728429	None:intergenic	30.0%
	TTGGGAACAACTTATGATAT+GGG	-	chr4_2:50728465-50728484	Msa0604960:intron	30.0%
	TCATATTCATAAATCTGAGC+TGG	-	chr4_2:50728513-50728532	Msa0604960:intron	30.0%
	AGTGAAACGTAAATGTAAGT+AGG	+	chr4_2:50728622-50728641	None:intergenic	30.0%
	TACTGACAAGAAAATAGAGT+TGG	+	chr4_2:50728800-50728819	None:intergenic	30.0%
!!	TTTTTGAGCTAAGTCAATAG+AGG	-	chr4_2:50728900-50728919	Msa0604960:intron	30.0%
	CTAAGTCAATAGAGGTTAAT+TGG	-	chr4_2:50728908-50728927	Msa0604960:intron	30.0%
	TTTGATGCATTAGCAAACAA+TGG	-	chr4_2:50727356-50727375	Msa0604960:three_prime_UTR	30.0%
	TTGATGCATTAGCAAACAAT+GGG	-	chr4_2:50727357-50727376	Msa0604960:three_prime_UTR	30.0%
	CACAAACTAAACTCTTAAAC+CGG	-	chr4_2:50727394-50727413	Msa0604960:three_prime_UTR	30.0%
!	GTACCGTCTTTTCATAATAT+AGG	-	chr4_2:50727457-50727476	Msa0604960:three_prime_UTR	30.0%
!	TCGAAGGTTTGAGAATTTTA+GGG	+	chr4_2:50727502-50727521	None:intergenic	30.0%
	GATCTGAATTAGGGAATTTA+TGG	-	chr4_2:50727815-50727834	Msa0604960:intron	30.0%
!!	TCAAACTTGGTAGTTGATAA+AGG	-	chr4_2:50727848-50727867	Msa0604960:intron	30.0%
!!	CAAACTTGGTAGTTGATAAA+GGG	-	chr4_2:50727849-50727868	Msa0604960:intron	30.0%
	AATTCTCAAAAACTAACCTG+GGG	+	chr4_2:50728167-50728186	None:intergenic	30.0%
!	TTATCGTTGGTTGTATGTTA+TGG	-	chr4_2:50728185-50728204	Msa0604960:CDS	30.0%
	ATAATAAGGGGATTGTGTAA+TGG	-	chr4_2:50728355-50728374	Msa0604960:intron	30.0%
	TGTAATGGCCAAATAGATAT+GGG	-	chr4_2:50728370-50728389	Msa0604960:intron	30.0%
	ATATTCTCCCCATATCTATT+TGG	+	chr4_2:50728381-50728400	None:intergenic	30.0%
	TTATGAATAAGAAATGGCAG+AGG	+	chr4_2:50728410-50728429	None:intergenic	30.0%
	TTGGGAACAACTTATGATAT+GGG	-	chr4_2:50728465-50728484	Msa0604960:intron	30.0%
	TCATATTCATAAATCTGAGC+TGG	-	chr4_2:50728513-50728532	Msa0604960:intron	30.0%
	AGTGAAACGTAAATGTAAGT+AGG	+	chr4_2:50728622-50728641	None:intergenic	30.0%
	TACTGACAAGAAAATAGAGT+TGG	+	chr4_2:50728800-50728819	None:intergenic	30.0%
!!	TTTTTGAGCTAAGTCAATAG+AGG	-	chr4_2:50728900-50728919	Msa0604960:intron	30.0%
	CTAAGTCAATAGAGGTTAAT+TGG	-	chr4_2:50728908-50728927	Msa0604960:intron	30.0%
	GTCGTACATTTGTTTCCAAA+AGG	-	chr4_2:50727296-50727315	Msa0604960:three_prime_UTR	35.0%
	TGATGCATTAGCAAACAATG+GGG	-	chr4_2:50727358-50727377	Msa0604960:three_prime_UTR	35.0%
!	CTCGAAGGTTTGAGAATTTT+AGG	+	chr4_2:50727503-50727522	None:intergenic	35.0%
	TCCTCCCCAAATATTACAAT+CGG	+	chr4_2:50727530-50727549	None:intergenic	35.0%
	GAAACACTGCAAAGAATCAA+TGG	+	chr4_2:50727598-50727617	None:intergenic	35.0%
	CTGTGTTGTGATTTGAAAGA+TGG	-	chr4_2:50727926-50727945	Msa0604960:intron	35.0%
	TTTGCTTCTCTTGATCTTCA+CGG	+	chr4_2:50728050-50728069	None:intergenic	35.0%
	CAAAGATGTTGTCAAGTTCA+AGG	-	chr4_2:50728066-50728085	Msa0604960:intron	35.0%
	ACAAAGTGTGTACAAGTACT+TGG	+	chr4_2:50728101-50728120	None:intergenic	35.0%
	ATTCTCAAAAACTAACCTGG+GGG	+	chr4_2:50728166-50728185	None:intergenic	35.0%
!	TTCTGTTGGTTGTTATAGGT+CGG	-	chr4_2:50728285-50728304	Msa0604960:intron	35.0%
	GTGTAATGGCCAAATAGATA+TGG	-	chr4_2:50728369-50728388	Msa0604960:intron	35.0%
	GTAATGGCCAAATAGATATG+GGG	-	chr4_2:50728371-50728390	Msa0604960:intron	35.0%
!!!	TGCTATTTTTCTCTCTCAGT+TGG	-	chr4_2:50728446-50728465	Msa0604960:intron	35.0%
!!!	GCTATTTTTCTCTCTCAGTT+GGG	-	chr4_2:50728447-50728466	Msa0604960:intron	35.0%
	GTTGGGAACAACTTATGATA+TGG	-	chr4_2:50728464-50728483	Msa0604960:intron	35.0%
	TGGGAACAACTTATGATATG+GGG	-	chr4_2:50728466-50728485	Msa0604960:intron	35.0%
!!	CATTTACGTTTCACTTTTGC+AGG	-	chr4_2:50728627-50728646	Msa0604960:intron	35.0%
	GCAAGACTTGTAATTCTATC+CGG	-	chr4_2:50728659-50728678	Msa0604960:intron	35.0%
	TCACTCAGTACACATTTAAC+CGG	+	chr4_2:50728681-50728700	None:intergenic	35.0%
	CTTACGACTCTCATTATGAA+TGG	-	chr4_2:50728773-50728792	Msa0604960:intron	35.0%
	GTCAATAGAGGTTAATTGGA+TGG	-	chr4_2:50728912-50728931	Msa0604960:intron	35.0%
	GTCGTACATTTGTTTCCAAA+AGG	-	chr4_2:50727296-50727315	Msa0604960:three_prime_UTR	35.0%
	TGATGCATTAGCAAACAATG+GGG	-	chr4_2:50727358-50727377	Msa0604960:three_prime_UTR	35.0%
!	CTCGAAGGTTTGAGAATTTT+AGG	+	chr4_2:50727503-50727522	None:intergenic	35.0%
	TCCTCCCCAAATATTACAAT+CGG	+	chr4_2:50727530-50727549	None:intergenic	35.0%
	GAAACACTGCAAAGAATCAA+TGG	+	chr4_2:50727598-50727617	None:intergenic	35.0%
	CTGTGTTGTGATTTGAAAGA+TGG	-	chr4_2:50727926-50727945	Msa0604960:intron	35.0%
	TTTGCTTCTCTTGATCTTCA+CGG	+	chr4_2:50728050-50728069	None:intergenic	35.0%
	CAAAGATGTTGTCAAGTTCA+AGG	-	chr4_2:50728066-50728085	Msa0604960:intron	35.0%
	ACAAAGTGTGTACAAGTACT+TGG	+	chr4_2:50728101-50728120	None:intergenic	35.0%
	ATTCTCAAAAACTAACCTGG+GGG	+	chr4_2:50728166-50728185	None:intergenic	35.0%
!	TTCTGTTGGTTGTTATAGGT+CGG	-	chr4_2:50728285-50728304	Msa0604960:intron	35.0%
	GTGTAATGGCCAAATAGATA+TGG	-	chr4_2:50728369-50728388	Msa0604960:intron	35.0%
	GTAATGGCCAAATAGATATG+GGG	-	chr4_2:50728371-50728390	Msa0604960:intron	35.0%
!!!	TGCTATTTTTCTCTCTCAGT+TGG	-	chr4_2:50728446-50728465	Msa0604960:intron	35.0%
!!!	GCTATTTTTCTCTCTCAGTT+GGG	-	chr4_2:50728447-50728466	Msa0604960:intron	35.0%
	GTTGGGAACAACTTATGATA+TGG	-	chr4_2:50728464-50728483	Msa0604960:intron	35.0%
	TGGGAACAACTTATGATATG+GGG	-	chr4_2:50728466-50728485	Msa0604960:intron	35.0%
!!	CATTTACGTTTCACTTTTGC+AGG	-	chr4_2:50728627-50728646	Msa0604960:intron	35.0%
	GCAAGACTTGTAATTCTATC+CGG	-	chr4_2:50728659-50728678	Msa0604960:intron	35.0%
	TCACTCAGTACACATTTAAC+CGG	+	chr4_2:50728681-50728700	None:intergenic	35.0%
	CTTACGACTCTCATTATGAA+TGG	-	chr4_2:50728773-50728792	Msa0604960:intron	35.0%
	GTCAATAGAGGTTAATTGGA+TGG	-	chr4_2:50728912-50728931	Msa0604960:intron	35.0%
	GATGCATTAGCAAACAATGG+GGG	-	chr4_2:50727359-50727378	Msa0604960:three_prime_UTR	40.0%
	TACTATCCTTATCTCAACGC+GGG	+	chr4_2:50727440-50727459	None:intergenic	40.0%
	GTACTATCCTTATCTCAACG+CGG	+	chr4_2:50727441-50727460	None:intergenic	40.0%
!!	AGCGTGCCGATTGTAATATT+TGG	-	chr4_2:50727521-50727540	Msa0604960:three_prime_UTR	40.0%
!!	GCGTGCCGATTGTAATATTT+GGG	-	chr4_2:50727522-50727541	Msa0604960:three_prime_UTR	40.0%
!!	CGTGCCGATTGTAATATTTG+GGG	-	chr4_2:50727523-50727542	Msa0604960:three_prime_UTR	40.0%
	TGTAATATTTGGGGAGGAAG+AGG	-	chr4_2:50727532-50727551	Msa0604960:three_prime_UTR	40.0%
	ATCGCAGCGAAGACAAAAAA+TGG	-	chr4_2:50727557-50727576	Msa0604960:three_prime_UTR	40.0%
	GTGCAAGTACTTTCAACTCT+AGG	-	chr4_2:50728230-50728249	Msa0604960:CDS	40.0%
	AGTACTTTCAACTCTAGGTG+AGG	-	chr4_2:50728235-50728254	Msa0604960:CDS	40.0%
	GTTATAGGTCGGAACCTTAA+AGG	-	chr4_2:50728296-50728315	Msa0604960:intron	40.0%
!!	GTGAAGATCAAGTGTTGCTT+TGG	-	chr4_2:50728697-50728716	Msa0604960:intron	40.0%
	TCATAATGAGAGTCGTAAGC+TGG	+	chr4_2:50728772-50728791	None:intergenic	40.0%
	GATGCATTAGCAAACAATGG+GGG	-	chr4_2:50727359-50727378	Msa0604960:three_prime_UTR	40.0%
	TACTATCCTTATCTCAACGC+GGG	+	chr4_2:50727440-50727459	None:intergenic	40.0%
	GTACTATCCTTATCTCAACG+CGG	+	chr4_2:50727441-50727460	None:intergenic	40.0%
!!	AGCGTGCCGATTGTAATATT+TGG	-	chr4_2:50727521-50727540	Msa0604960:three_prime_UTR	40.0%
!!	GCGTGCCGATTGTAATATTT+GGG	-	chr4_2:50727522-50727541	Msa0604960:three_prime_UTR	40.0%
!!	CGTGCCGATTGTAATATTTG+GGG	-	chr4_2:50727523-50727542	Msa0604960:three_prime_UTR	40.0%
	TGTAATATTTGGGGAGGAAG+AGG	-	chr4_2:50727532-50727551	Msa0604960:three_prime_UTR	40.0%
	ATCGCAGCGAAGACAAAAAA+TGG	-	chr4_2:50727557-50727576	Msa0604960:three_prime_UTR	40.0%
	GTGCAAGTACTTTCAACTCT+AGG	-	chr4_2:50728230-50728249	Msa0604960:CDS	40.0%
	AGTACTTTCAACTCTAGGTG+AGG	-	chr4_2:50728235-50728254	Msa0604960:CDS	40.0%
	GTTATAGGTCGGAACCTTAA+AGG	-	chr4_2:50728296-50728315	Msa0604960:intron	40.0%
!!	GTGAAGATCAAGTGTTGCTT+TGG	-	chr4_2:50728697-50728716	Msa0604960:intron	40.0%
	TCATAATGAGAGTCGTAAGC+TGG	+	chr4_2:50728772-50728791	None:intergenic	40.0%
	ACTCTTAAACCGGCCCAATA+CGG	-	chr4_2:50727404-50727423	Msa0604960:three_prime_UTR	45.0%
!	TTTTTCCTTCCGTATTGGGC+CGG	+	chr4_2:50727416-50727435	None:intergenic	45.0%
!!	GGGCTTTTTCCTTCCGTATT+GGG	+	chr4_2:50727420-50727439	None:intergenic	45.0%
	AAAAAGCCCGCGTTGAGATA+AGG	-	chr4_2:50727431-50727450	Msa0604960:three_prime_UTR	45.0%
	GCCGATTGTAATATTTGGGG+AGG	-	chr4_2:50727526-50727545	Msa0604960:three_prime_UTR	45.0%
	ACTCTTAAACCGGCCCAATA+CGG	-	chr4_2:50727404-50727423	Msa0604960:three_prime_UTR	45.0%
!	TTTTTCCTTCCGTATTGGGC+CGG	+	chr4_2:50727416-50727435	None:intergenic	45.0%
!!	GGGCTTTTTCCTTCCGTATT+GGG	+	chr4_2:50727420-50727439	None:intergenic	45.0%
	AAAAAGCCCGCGTTGAGATA+AGG	-	chr4_2:50727431-50727450	Msa0604960:three_prime_UTR	45.0%
	GCCGATTGTAATATTTGGGG+AGG	-	chr4_2:50727526-50727545	Msa0604960:three_prime_UTR	45.0%
	TTAAACCGGCCCAATACGGA+AGG	-	chr4_2:50727408-50727427	Msa0604960:three_prime_UTR	50.0%
!!	CGGGCTTTTTCCTTCCGTAT+TGG	+	chr4_2:50727421-50727440	None:intergenic	50.0%
	ATTACAATCGGCACGCTCGA+AGG	+	chr4_2:50727518-50727537	None:intergenic	50.0%
	TTGAAGCAATCACTTCCCCC+AGG	-	chr4_2:50728148-50728167	Msa0604960:CDS	50.0%
	TTAAACCGGCCCAATACGGA+AGG	-	chr4_2:50727408-50727427	Msa0604960:three_prime_UTR	50.0%
!!	CGGGCTTTTTCCTTCCGTAT+TGG	+	chr4_2:50727421-50727440	None:intergenic	50.0%
	ATTACAATCGGCACGCTCGA+AGG	+	chr4_2:50727518-50727537	None:intergenic	50.0%
	TTGAAGCAATCACTTCCCCC+AGG	-	chr4_2:50728148-50728167	Msa0604960:CDS	50.0%
	AGGACTTCCTCCTCACTGCT+AGG	-	chr4_2:50728008-50728027	Msa0604960:intron	55.0%
	ACTTCCTCCTCACTGCTAGG+AGG	-	chr4_2:50728011-50728030	Msa0604960:intron	55.0%
	CATCCTTCCTCCTAGCAGTG+AGG	+	chr4_2:50728021-50728040	None:intergenic	55.0%
	AGGACTTCCTCCTCACTGCT+AGG	-	chr4_2:50728008-50728027	Msa0604960:intron	55.0%
	ACTTCCTCCTCACTGCTAGG+AGG	-	chr4_2:50728011-50728030	Msa0604960:intron	55.0%
	CATCCTTCCTCCTAGCAGTG+AGG	+	chr4_2:50728021-50728040	None:intergenic	55.0%
	CCTTCCTCCTAGCAGTGAGG+AGG	+	chr4_2:50728018-50728037	None:intergenic	60.0%
	CCTCCTCACTGCTAGGAGGA+AGG	-	chr4_2:50728015-50728034	Msa0604960:intron	60.0%
	CCTTCCTCCTAGCAGTGAGG+AGG	+	chr4_2:50728018-50728037	None:intergenic	60.0%
	CCTCCTCACTGCTAGGAGGA+AGG	-	chr4_2:50728015-50728034	Msa0604960:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	50727292	50728983	50727292	ID=Msa0604960;Name=Msa0604960
chr4_2	mRNA	50727292	50728983	50727292	ID=Msa0604960-mRNA-1;Parent=Msa0604960;Name=Msa0604960-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0.5\|0.66\|0.66\|1\|1\|3\|312\|79
chr4_2	exon	50727292	50727626	50727292	ID=Msa0604960-mRNA-1:exon:12034;Parent=Msa0604960-mRNA-1
chr4_2	exon	50728106	50728289	50728106	ID=Msa0604960-mRNA-1:exon:12033;Parent=Msa0604960-mRNA-1
chr4_2	exon	50728951	50728983	50728951	ID=Msa0604960-mRNA-1:exon:12032;Parent=Msa0604960-mRNA-1
chr4_2	CDS	50728951	50728983	50728951	ID=Msa0604960-mRNA-1:cds;Parent=Msa0604960-mRNA-1
chr4_2	CDS	50728106	50728289	50728106	ID=Msa0604960-mRNA-1:cds;Parent=Msa0604960-mRNA-1
chr4_2	CDS	50727604	50727626	50727604	ID=Msa0604960-mRNA-1:cds;Parent=Msa0604960-mRNA-1
chr4_2	three_prime_UTR	50727292	50727603	50727292	ID=Msa0604960-mRNA-1:three_prime_utr;Parent=Msa0604960-mRNA-1

Gene Sequence	>Msa0604960 ATGGGTCGTACATTTGTTTCCAAAAGGAAAAATCCTAAGCAAATCAATGAGATTAAGGACTTCCTCCTCACTGCTAGGAGGAAGGATGCACGCTCCGTGAAGATCAAGAGAAGCAAAGATGTTGTCAAGTTCAAGGTTCGATGCTCCAAGTACTTGTACACACTTTGTGTGTTTGATACTGAGAAAGCTGATAAGTTGAAGCAATCACTTCCCCCAGGTTTGAATGTGCAAGACTTGTAA
Protein sequence	>Msa0604960 MGRTFVSKRKNPKQINEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDTEKADKLKQSLPPGLNVQDL*