AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1125580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125580 MtrunA17_Chr7g0266271 100.000 72 0 0 1 72 1 72 6.76e-48 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125580 AT3G52730.1 80.556 72 14 0 1 72 1 72 7.42e-39 123
Msa1125580 AT3G52730.2 76.786 56 13 0 1 56 1 56 1.07e-26 92.8

Find 52 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTCACTTATTGCAAAA+TGG 0.252781 7_3:+77052032 None:intergenic
TCCGATCTGATTTGAGTTCT+AGG 0.278024 7_3:+77054542 None:intergenic
ATATGATTTATCCCAAATAC+TGG 0.293371 7_3:-77051913 Msa1125580:three_prime_UTR
TCATGTATGGTTTCATTTGC+TGG 0.324683 7_3:-77051988 Msa1125580:three_prime_UTR
AAGAAGAGGAGGAGGCGTTT+TGG 0.335531 7_3:-77054499 Msa1125580:CDS
TTAATTAAATTAAATTAAAT+CGG 0.340843 7_3:+77051833 None:intergenic
AATATAATAAAACTTCAAAA+TGG 0.380237 7_3:+77052095 None:intergenic
CAGCTGCTTTGAATTTCAGC+AGG 0.381477 7_3:+77051764 None:intergenic
TCTGGGAAATAAATAATGTT+GGG 0.387527 7_3:-77054289 Msa1125580:CDS
TGTTGACCTTAAGTCATGTA+TGG 0.394843 7_3:-77052001 Msa1125580:three_prime_UTR
TCGTTGCAGGCTGTGGATTA+CGG 0.406296 7_3:-77054323 Msa1125580:intron
TCAATAGCGAATGCGGAAAC+AGG 0.413736 7_3:-77054698 None:intergenic
ATTACGGTGTTCACAAGCTC+TGG 0.424543 7_3:-77054307 Msa1125580:CDS
TACATTTGAGAACGGTGAAA+AGG 0.431138 7_3:-77054608 Msa1125580:five_prime_UTR
ATGAAACCATACATGACTTA+AGG 0.434265 7_3:+77051995 None:intergenic
CTCTGGGAAATAAATAATGT+TGG 0.443669 7_3:-77054290 Msa1125580:CDS
CCGATCTGATTTGAGTTCTA+GGG 0.456736 7_3:+77054543 None:intergenic
TTCTGTGCTGGGGCAAAGGC+CGG 0.481651 7_3:-77052150 Msa1125580:CDS
TATGTTACCTTCGTCATCGC+CGG 0.481783 7_3:-77054438 Msa1125580:CDS
ATATGAAGACATTTCTGTGC+TGG 0.485619 7_3:-77052162 Msa1125580:CDS
ATGCGGAAACAGGCAGTGTC+CGG 0.495979 7_3:-77054688 Msa1125580:five_prime_UTR
TATGAAGACATTTCTGTGCT+GGG 0.504170 7_3:-77052161 Msa1125580:CDS
ATTAAATTAAATTAAATCGG+TGG 0.504656 7_3:+77051836 None:intergenic
TGGTTTCATTTGCTGGCTGT+TGG 0.509038 7_3:-77051981 Msa1125580:three_prime_UTR
ACATTTCTGTGCTGGGGCAA+AGG 0.516213 7_3:-77052154 Msa1125580:CDS
ACATTTGAGAACGGTGAAAA+GGG 0.519176 7_3:-77054607 Msa1125580:five_prime_UTR
CAGACAGTTCACTCGTCTAC+CGG 0.527733 7_3:+77052131 None:intergenic
TTAAATTAAATTAAATCGGT+GGG 0.527868 7_3:+77051837 None:intergenic
CCGGTACGGTTCGTTTGAAA+CGG 0.532497 7_3:-77054655 Msa1125580:five_prime_UTR
CCGTTTCAAACGAACCGTAC+CGG 0.537500 7_3:+77054655 None:intergenic
CTCAAATCAGATCGGAACCA+TGG 0.553505 7_3:-77054535 Msa1125580:exon
ATTGAAGTGAACTTACGAGC+TGG 0.554063 7_3:-77052064 Msa1125580:three_prime_UTR
CCGGTCGGATCGAGCCGGTA+CGG 0.558201 7_3:-77054669 Msa1125580:five_prime_UTR
CCTTTGTTTACATTTGAGAA+CGG 0.567826 7_3:-77054616 Msa1125580:five_prime_UTR
AGCTGCTTTGAATTTCAGCA+GGG 0.588712 7_3:+77051765 None:intergenic
TTACGGTGTTCACAAGCTCT+GGG 0.588806 7_3:-77054306 Msa1125580:CDS
CCGTACCGGCTCGATCCGAC+CGG 0.589872 7_3:+77054669 None:intergenic
CCCTAGAACTCAAATCAGAT+CGG 0.590863 7_3:-77054543 Msa1125580:five_prime_UTR
AGTGTCCGGTCGGATCGAGC+CGG 0.597440 7_3:-77054674 Msa1125580:five_prime_UTR
CTGGGAAATAAATAATGTTG+GGG 0.603916 7_3:-77054288 Msa1125580:intron
GGCGCATAAGAACCTTGTAA+AGG 0.605705 7_3:+77054472 None:intergenic
CAGCCCATCAATAGCGAATG+CGG 0.615932 7_3:-77054705 None:intergenic
CCGTTCTCAAATGTAAACAA+AGG 0.621436 7_3:+77054616 None:intergenic
GCTGCTAAAAGAAGAGGAGG+AGG 0.621931 7_3:-77054507 Msa1125580:CDS
GAATCCGCTGCTAAAAGAAG+AGG 0.626583 7_3:-77054513 Msa1125580:CDS
AAAAGCACCGGCGATGACGA+AGG 0.627325 7_3:+77054431 None:intergenic
TCCGCTGCTAAAAGAAGAGG+AGG 0.631604 7_3:-77054510 Msa1125580:CDS
ATGAAGACATTTCTGTGCTG+GGG 0.643761 7_3:-77052160 Msa1125580:CDS
CCGTTCACCGAGAAAAGCAC+CGG 0.644147 7_3:+77054419 None:intergenic
GGAAACAGGCAGTGTCCGGT+CGG 0.659778 7_3:-77054684 Msa1125580:five_prime_UTR
GCTGCTTTGAATTTCAGCAG+GGG 0.684683 7_3:+77051766 None:intergenic
AGGTAACATAGACAGAGTTG+CGG 0.713031 7_3:+77054451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAAATTAAATTAAAT+CGG + chr7_3:77054613-77054632 None:intergenic 0.0%
!! AATATAATAAAACTTCAAAA+TGG + chr7_3:77054351-77054370 None:intergenic 10.0%
!! ATTAAGAGTAAAATTAAAAG+GGG + chr7_3:77052063-77052082 None:intergenic 15.0%
!! GATTAAGAGTAAAATTAAAA+GGG + chr7_3:77052064-77052083 None:intergenic 15.0%
!! TACTATTATATAACTTTGAA+AGG - chr7_3:77052291-77052310 Msa1125580:intron 15.0%
!!! ACTATTATATAACTTTGAAA+GGG - chr7_3:77052292-77052311 Msa1125580:intron 15.0%
!!! TTTTTATAGAATAATCTCTT+AGG - chr7_3:77052386-77052405 Msa1125580:intron 15.0%
!! AATATAATAACAACATAGAT+TGG + chr7_3:77052956-77052975 None:intergenic 15.0%
!!! TTGAAGTTTTATTATATTCA+TGG - chr7_3:77054353-77054372 Msa1125580:intron 15.0%
!! TTAAATTAAATTAAATCGGT+GGG + chr7_3:77054609-77054628 None:intergenic 15.0%
!! ATTAAATTAAATTAAATCGG+TGG + chr7_3:77054610-77054629 None:intergenic 15.0%
!! CGATTAAGAGTAAAATTAAA+AGG + chr7_3:77052065-77052084 None:intergenic 20.0%
!!! GCTTATGAAATTTGTATTTT+TGG - chr7_3:77052320-77052339 Msa1125580:intron 20.0%
!! GTTGGAAATATATAGAAAAA+TGG + chr7_3:77053063-77053082 None:intergenic 20.0%
!! AATTACTTCATATAAACAAC+AGG + chr7_3:77054019-77054038 None:intergenic 20.0%
!! TTGTTTATATGAAGTAATTC+TGG - chr7_3:77054020-77054039 Msa1125580:intron 20.0%
!! CTTGAAAAAAAAGAAGATAA+AGG - chr7_3:77054137-77054156 Msa1125580:intron 20.0%
!!! GTTATCTGAAATTAAGTTAA+AGG - chr7_3:77054168-77054187 Msa1125580:intron 20.0%
!! GTAAAAATATGAATCACAAA+TGG + chr7_3:77054227-77054246 None:intergenic 20.0%
!!! ATTTTCTTAATCCAGTATTT+GGG + chr7_3:77054544-77054563 None:intergenic 20.0%
! TCTGGGAAATAAATAATGTT+GGG - chr7_3:77052154-77052173 Msa1125580:CDS 25.0%
!! ATATAACTTTGAAAGGGATT+AGG - chr7_3:77052298-77052317 Msa1125580:intron 25.0%
!!! TGTTGAAATGTTTTTTCCTT+GGG - chr7_3:77052363-77052382 Msa1125580:intron 25.0%
! GATTATTCTATAAAAACCCA+AGG + chr7_3:77052382-77052401 None:intergenic 25.0%
!! TGTTATCTCTTTGAAGAATA+TGG - chr7_3:77052589-77052608 Msa1125580:intron 25.0%
! TCTATTCCTATTATTCATTG+TGG - chr7_3:77052679-77052698 Msa1125580:intron 25.0%
! CATCACTATTTCAAAAAAGA+TGG + chr7_3:77053215-77053234 None:intergenic 25.0%
!!! TTTTGAAATAGTGATGTTGT+AGG - chr7_3:77053219-77053238 Msa1125580:intron 25.0%
!! TTTGAAATAGTGATGTTGTA+GGG - chr7_3:77053220-77053239 Msa1125580:intron 25.0%
!! AAAATTGTATTGTGGGATTT+TGG + chr7_3:77053339-77053358 None:intergenic 25.0%
! GAACACAAAAATTGTATTGT+GGG + chr7_3:77053346-77053365 None:intergenic 25.0%
!!! CATTAATCTCTACTTTGTTT+TGG - chr7_3:77053587-77053606 Msa1125580:intron 25.0%
!!! TTTGTTTTGGTTGTTTCTTT+TGG - chr7_3:77053600-77053619 Msa1125580:intron 25.0%
!!! TTTGGTTCTTTTTCACTAAT+CGG - chr7_3:77053618-77053637 Msa1125580:intron 25.0%
! ATATGTTAACATGATCAAGT+AGG + chr7_3:77053699-77053718 None:intergenic 25.0%
! AATTACTTCGTAAAAACAAC+AGG + chr7_3:77053793-77053812 None:intergenic 25.0%
!!! TTGTTTTTACGAAGTAATTC+TGG - chr7_3:77053794-77053813 Msa1125580:intron 25.0%
! CATATTCACTTATTGCAAAA+TGG + chr7_3:77054414-77054433 None:intergenic 25.0%
! ATATGATTTATCCCAAATAC+TGG - chr7_3:77054530-77054549 Msa1125580:exon 25.0%
!! GATTTTCTTAATCCAGTATT+TGG + chr7_3:77054545-77054564 None:intergenic 25.0%
! CCTTTGTTTACATTTGAGAA+CGG - chr7_3:77051827-77051846 Msa1125580:three_prime_UTR 30.0%
! CTCTGGGAAATAAATAATGT+TGG - chr7_3:77052153-77052172 Msa1125580:CDS 30.0%
CTGGGAAATAAATAATGTTG+GGG - chr7_3:77052155-77052174 Msa1125580:CDS 30.0%
TCGATAGATTAAGCATAAAG+TGG + chr7_3:77052194-77052213 None:intergenic 30.0%
!!! GTGTTGAAATGTTTTTTCCT+TGG - chr7_3:77052362-77052381 Msa1125580:intron 30.0%
! GATTCTGATTTCGTGTTTTA+TGG + chr7_3:77052490-77052509 None:intergenic 30.0%
TAAGTTATGTCGTGTGTTAT+TGG - chr7_3:77052558-77052577 Msa1125580:intron 30.0%
ATATAACAGACAACTGTGTA+AGG - chr7_3:77052635-77052654 Msa1125580:intron 30.0%
! TTGTCATCTGATTTTCTCTT+AGG - chr7_3:77052720-77052739 Msa1125580:intron 30.0%
!!! ATTTTCTCTTAGGCTTTGTT+TGG - chr7_3:77052730-77052749 Msa1125580:intron 30.0%
!!! TTTTCTCTTAGGCTTTGTTT+GGG - chr7_3:77052731-77052750 Msa1125580:intron 30.0%
TGGAAGAAATAGAAGACATT+GGG - chr7_3:77052804-77052823 Msa1125580:intron 30.0%
AATAAAAAATCCCCCTCATT+TGG - chr7_3:77052861-77052880 Msa1125580:intron 30.0%
ATAAAAAATCCCCCTCATTT+GGG - chr7_3:77052862-77052881 Msa1125580:intron 30.0%
GGGAACTCAAAAATTGTATT+GGG - chr7_3:77052884-77052903 Msa1125580:intron 30.0%
GGAACTCAAAAATTGTATTG+GGG - chr7_3:77052885-77052904 Msa1125580:intron 30.0%
GAACTCAAAAATTGTATTGG+GGG - chr7_3:77052886-77052905 Msa1125580:intron 30.0%
AAACTTGCAAATAGAACCTA+AGG + chr7_3:77053132-77053151 None:intergenic 30.0%
TTAGGTTCTATTTGCAAGTT+TGG - chr7_3:77053131-77053150 Msa1125580:intron 30.0%
GGAACACAAAAATTGTATTG+TGG + chr7_3:77053347-77053366 None:intergenic 30.0%
AAAACAAAATACCCTCAACT+GGG + chr7_3:77053370-77053389 None:intergenic 30.0%
TAAAACAAAATACCCTCAAC+TGG + chr7_3:77053371-77053390 None:intergenic 30.0%
!!! GTTGAGGGTATTTTGTTTTA+TGG - chr7_3:77053371-77053390 Msa1125580:intron 30.0%
AAAATTGTTTCAACAGGCTT+TGG + chr7_3:77053732-77053751 None:intergenic 30.0%
TCCAGTAAAATTGTTTCAAC+AGG + chr7_3:77053738-77053757 None:intergenic 30.0%
TTTCATTGTTTACGATGCTT+TGG - chr7_3:77053881-77053900 Msa1125580:intron 30.0%
!! ATGACTCTTCTTTTGAAGAA+GGG + chr7_3:77053912-77053931 None:intergenic 30.0%
!! TATGACTCTTCTTTTGAAGA+AGG + chr7_3:77053913-77053932 None:intergenic 30.0%
AAAAGTGACACTAAGCAATT+GGG + chr7_3:77053984-77054003 None:intergenic 30.0%
TTTCATTGTTTACGATGCTT+TGG - chr7_3:77053881-77053900 Msa1125580:intron 30.0%
ATGAAACCATACATGACTTA+AGG + chr7_3:77054451-77054470 None:intergenic 30.0%
CCGTTCTCAAATGTAAACAA+AGG + chr7_3:77051830-77051849 None:intergenic 35.0%
TACATTTGAGAACGGTGAAA+AGG - chr7_3:77051835-77051854 Msa1125580:three_prime_UTR 35.0%
ACATTTGAGAACGGTGAAAA+GGG - chr7_3:77051836-77051855 Msa1125580:three_prime_UTR 35.0%
!!! GTTTCGCTAGTTTTAAGATC+TGG - chr7_3:77052267-77052286 Msa1125580:intron 35.0%
!! GTGTGATTGTTGCTTCATTT+TGG - chr7_3:77052435-77052454 Msa1125580:intron 35.0%
! TCTGATTTCGTGTTTTATGG+TGG + chr7_3:77052487-77052506 None:intergenic 35.0%
GAAACAACAATCACAGTTAC+AGG + chr7_3:77052522-77052541 None:intergenic 35.0%
ATGCAGCCACAATGAATAAT+AGG + chr7_3:77052688-77052707 None:intergenic 35.0%
TTATTCATTGTGGCTGCATT+AGG - chr7_3:77052689-77052708 Msa1125580:intron 35.0%
!!! GAGAGTTTTGGAAAAAAGGA+AGG - chr7_3:77052775-77052794 Msa1125580:intron 35.0%
GTGGAAGAAATAGAAGACAT+TGG - chr7_3:77052803-77052822 Msa1125580:intron 35.0%
AAGAAATAGAAGACATTGGG+AGG - chr7_3:77052807-77052826 Msa1125580:intron 35.0%
TAAAAAATCCCCCTCATTTG+GGG - chr7_3:77052863-77052882 Msa1125580:intron 35.0%
GGGGAACTCAAAAATTGTAT+TGG - chr7_3:77052883-77052902 Msa1125580:intron 35.0%
TCAAAAATTGTATTGGGGGA+GGG - chr7_3:77052890-77052909 Msa1125580:intron 35.0%
!! TAGAACCTAAGGCTTTGTTT+GGG + chr7_3:77053121-77053140 None:intergenic 35.0%
!! ATAGAACCTAAGGCTTTGTT+TGG + chr7_3:77053122-77053141 None:intergenic 35.0%
GTTCTATTTGCAAGTTTGGA+GGG - chr7_3:77053135-77053154 Msa1125580:intron 35.0%
TTCTATTTGCAAGTTTGGAG+GGG - chr7_3:77053136-77053155 Msa1125580:intron 35.0%
! TTGTATTGTGGGATTTTGGA+AGG + chr7_3:77053335-77053354 None:intergenic 35.0%
AAACAAAATACCCTCAACTG+GGG + chr7_3:77053369-77053388 None:intergenic 35.0%
! TGAAAGGAATGAAGGACTTT+TGG + chr7_3:77053427-77053446 None:intergenic 35.0%
ATATAAGCTGCAAACACCTA+AGG + chr7_3:77053489-77053508 None:intergenic 35.0%
AGCAGAGCAGGTATATAATT+TGG + chr7_3:77053541-77053560 None:intergenic 35.0%
!! CTTTTTCACTAATCGGTAAC+CGG - chr7_3:77053625-77053644 Msa1125580:intron 35.0%
AGGAAACAATGAGTGAAAGT+CGG + chr7_3:77053668-77053687 None:intergenic 35.0%
! GCCTGTTGAAACAATTTTAC+TGG - chr7_3:77053734-77053753 Msa1125580:intron 35.0%
AAAGTGACACTAAGCAATTG+GGG + chr7_3:77053983-77054002 None:intergenic 35.0%
CAAAAGTGACACTAAGCAAT+TGG + chr7_3:77053985-77054004 None:intergenic 35.0%
ATATGAAGACATTTCTGTGC+TGG - chr7_3:77054281-77054300 Msa1125580:intron 35.0%
TATGAAGACATTTCTGTGCT+GGG - chr7_3:77054282-77054301 Msa1125580:intron 35.0%
TGTTGACCTTAAGTCATGTA+TGG - chr7_3:77054442-77054461 Msa1125580:CDS 35.0%
TCATGTATGGTTTCATTTGC+TGG - chr7_3:77054455-77054474 Msa1125580:CDS 35.0%
! CCGATCTGATTTGAGTTCTA+GGG + chr7_3:77051903-77051922 None:intergenic 40.0%
CCCTAGAACTCAAATCAGAT+CGG - chr7_3:77051900-77051919 Msa1125580:three_prime_UTR 40.0%
! TCCGATCTGATTTGAGTTCT+AGG + chr7_3:77051904-77051923 None:intergenic 40.0%
AGGTAACATAGACAGAGTTG+CGG + chr7_3:77051995-77052014 None:intergenic 40.0%
!! TTCATTTTGGCATGATACCG+TGG - chr7_3:77052448-77052467 Msa1125580:intron 40.0%
CAGACAACTGTGTAAGGAAA+TGG - chr7_3:77052641-77052660 Msa1125580:intron 40.0%
!! TTAGGCTTTGTTTGGGAGTT+TGG - chr7_3:77052738-77052757 Msa1125580:intron 40.0%
!!! AGGGGAGAGTTTTGGAAAAA+AGG - chr7_3:77052771-77052790 Msa1125580:intron 40.0%
AAAAAATCCCCCTCATTTGG+GGG - chr7_3:77052864-77052883 Msa1125580:intron 40.0%
!!! TTTTGAGTTCCCCCAAATGA+GGG + chr7_3:77052876-77052895 None:intergenic 40.0%
!!! TTTTTGAGTTCCCCCAAATG+AGG + chr7_3:77052877-77052896 None:intergenic 40.0%
CTCAAAAATTGTATTGGGGG+AGG - chr7_3:77052889-77052908 Msa1125580:intron 40.0%
! CTTTGTTTGGGAGTTTGAAG+GGG + chr7_3:77053109-77053128 None:intergenic 40.0%
!! GCTTTGTTTGGGAGTTTGAA+GGG + chr7_3:77053110-77053129 None:intergenic 40.0%
AAACTCCCAAACAAAGCCTT+AGG - chr7_3:77053113-77053132 Msa1125580:intron 40.0%
GGTTCTATTTGCAAGTTTGG+AGG - chr7_3:77053134-77053153 Msa1125580:intron 40.0%
TCTATTTGCAAGTTTGGAGG+GGG - chr7_3:77053137-77053156 Msa1125580:intron 40.0%
AACAAAATACCCTCAACTGG+GGG + chr7_3:77053368-77053387 None:intergenic 40.0%
! AGGAATGAAGGACTTTTGGA+GGG + chr7_3:77053423-77053442 None:intergenic 40.0%
! AAGGAATGAAGGACTTTTGG+AGG + chr7_3:77053424-77053443 None:intergenic 40.0%
! GCTTTAGGTGAAAGGAATGA+AGG + chr7_3:77053435-77053454 None:intergenic 40.0%
! ATTTTGTGAGCTGCTGATCT+TGG - chr7_3:77053563-77053582 Msa1125580:intron 40.0%
! ACTAATCGGTAACCGGTTTA+TGG - chr7_3:77053632-77053651 Msa1125580:intron 40.0%
TGATCAAGTAGGATCAGTGT+AGG + chr7_3:77053688-77053707 None:intergenic 40.0%
TTGAATGCAAAAGTGACGCT+AGG + chr7_3:77053766-77053785 None:intergenic 40.0%
ATGAAGACATTTCTGTGCTG+GGG - chr7_3:77054283-77054302 Msa1125580:intron 40.0%
ATTGAAGTGAACTTACGAGC+TGG - chr7_3:77054379-77054398 Msa1125580:intron 40.0%
! AGCTGCTTTGAATTTCAGCA+GGG + chr7_3:77054681-77054700 None:intergenic 40.0%
CTCAAATCAGATCGGAACCA+TGG - chr7_3:77051908-77051927 Msa1125580:three_prime_UTR 45.0%
!! TCTTTTAGCAGCGGATTCCA+TGG + chr7_3:77051928-77051947 None:intergenic 45.0%
GAATCCGCTGCTAAAAGAAG+AGG - chr7_3:77051930-77051949 Msa1125580:three_prime_UTR 45.0%
! TCCTCCTCTTCTTTTAGCAG+CGG + chr7_3:77051937-77051956 None:intergenic 45.0%
!!! CGTTTTGGAAGGCCTTTACA+AGG - chr7_3:77051959-77051978 Msa1125580:three_prime_UTR 45.0%
GGCGCATAAGAACCTTGTAA+AGG + chr7_3:77051974-77051993 None:intergenic 45.0%
TATGTTACCTTCGTCATCGC+CGG - chr7_3:77052005-77052024 Msa1125580:three_prime_UTR 45.0%
AGATCGCTGAATTTCGTTGC+AGG - chr7_3:77052107-77052126 Msa1125580:three_prime_UTR 45.0%
ATTACGGTGTTCACAAGCTC+TGG - chr7_3:77052136-77052155 Msa1125580:exon 45.0%
TTACGGTGTTCACAAGCTCT+GGG - chr7_3:77052137-77052156 Msa1125580:exon 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG - chr7_3:77052742-77052761 Msa1125580:intron 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG - chr7_3:77052743-77052762 Msa1125580:intron 45.0%
GGAAAAAAGGAAGGAGCAAG+TGG - chr7_3:77052784-77052803 Msa1125580:intron 45.0%
ATAGAAGACATTGGGAGGAG+AGG - chr7_3:77052812-77052831 Msa1125580:intron 45.0%
TAGAAGACATTGGGAGGAGA+GGG - chr7_3:77052813-77052832 Msa1125580:intron 45.0%
! TTTGAGTTCCCCCAAATGAG+GGG + chr7_3:77052875-77052894 None:intergenic 45.0%
! ATTGTATTGGGGGAGGGTTT+TGG - chr7_3:77052896-77052915 Msa1125580:intron 45.0%
! TTGTATTGGGGGAGGGTTTT+GGG - chr7_3:77052897-77052916 Msa1125580:intron 45.0%
TTTGGGAGTTTGAAGGGGAA+GGG + chr7_3:77053104-77053123 None:intergenic 45.0%
!! GGCTTTGTTTGGGAGTTTGA+AGG + chr7_3:77053111-77053130 None:intergenic 45.0%
! TTTTTGTGTTCCCCCAGTTG+AGG - chr7_3:77053355-77053374 Msa1125580:intron 45.0%
! TTTTGTGTTCCCCCAGTTGA+GGG - chr7_3:77053356-77053375 Msa1125580:intron 45.0%
!! GGAATGAAGGACTTTTGGAG+GGG + chr7_3:77053422-77053441 None:intergenic 45.0%
!! AAGAGAGGGCTTTAGGTGAA+AGG + chr7_3:77053443-77053462 None:intergenic 45.0%
TAAGGCTCTGTTTGCGAGTT+TGG + chr7_3:77053471-77053490 None:intergenic 45.0%
AAACTCGCAAACAGAGCCTT+AGG - chr7_3:77053470-77053489 Msa1125580:intron 45.0%
CTCACAAAATTCAGCAGAGC+AGG + chr7_3:77053553-77053572 None:intergenic 45.0%
GGCAGTAGAGAACCATAAAC+CGG + chr7_3:77053647-77053666 None:intergenic 45.0%
! TGGTTTCATTTGCTGGCTGT+TGG - chr7_3:77054462-77054481 Msa1125580:CDS 45.0%
! GCTGCTTTGAATTTCAGCAG+GGG + chr7_3:77054680-77054699 None:intergenic 45.0%
! CAGCTGCTTTGAATTTCAGC+AGG + chr7_3:77054682-77054701 None:intergenic 45.0%
CCGTTTCAAACGAACCGTAC+CGG + chr7_3:77051791-77051810 None:intergenic 50.0%
CCGGTACGGTTCGTTTGAAA+CGG - chr7_3:77051788-77051807 Msa1125580:three_prime_UTR 50.0%
TCCGCTGCTAAAAGAAGAGG+AGG - chr7_3:77051933-77051952 Msa1125580:three_prime_UTR 50.0%
GCTGCTAAAAGAAGAGGAGG+AGG - chr7_3:77051936-77051955 Msa1125580:three_prime_UTR 50.0%
!!! AAGAAGAGGAGGAGGCGTTT+TGG - chr7_3:77051944-77051963 Msa1125580:three_prime_UTR 50.0%
CTGAATTTCGTTGCAGGCTG+TGG - chr7_3:77052113-77052132 Msa1125580:three_prime_UTR 50.0%
! TCGTTGCAGGCTGTGGATTA+CGG - chr7_3:77052120-77052139 Msa1125580:exon 50.0%
!! GGCTTTGTTTGGGAGTTTGG+AGG - chr7_3:77052741-77052760 Msa1125580:intron 50.0%
TTGAGTTCCCCCAAATGAGG+GGG + chr7_3:77052874-77052893 None:intergenic 50.0%
! TGTATTGGGGGAGGGTTTTG+GGG - chr7_3:77052898-77052917 Msa1125580:intron 50.0%
GTTTGGGAGTTTGAAGGGGA+AGG + chr7_3:77053105-77053124 None:intergenic 50.0%
ATTTGCAAGTTTGGAGGGGG+AGG - chr7_3:77053140-77053159 Msa1125580:intron 50.0%
TTTGCAAGTTTGGAGGGGGA+GGG - chr7_3:77053141-77053160 Msa1125580:intron 50.0%
!! TTTGGAGGGGATGGCTTTGA+GGG + chr7_3:77053409-77053428 None:intergenic 50.0%
!! TTTTGGAGGGGATGGCTTTG+AGG + chr7_3:77053410-77053429 None:intergenic 50.0%
!! TGAAGGACTTTTGGAGGGGA+TGG + chr7_3:77053418-77053437 None:intergenic 50.0%
CTCTGTTTGCGAGTTTGGAG+GGG + chr7_3:77053466-77053485 None:intergenic 50.0%
GCTCTGTTTGCGAGTTTGGA+GGG + chr7_3:77053467-77053486 None:intergenic 50.0%
ACATTTCTGTGCTGGGGCAA+AGG - chr7_3:77054289-77054308 Msa1125580:CDS 50.0%
CAGACAGTTCACTCGTCTAC+CGG + chr7_3:77054315-77054334 None:intergenic 50.0%
ATGCGGAAACAGGCAGTGTC+CGG - chr7_3:77051755-77051774 Msa1125580:three_prime_UTR 55.0%
!!! AGAGGAGGAGGCGTTTTGGA+AGG - chr7_3:77051948-77051967 Msa1125580:three_prime_UTR 55.0%
!! AAAAGCACCGGCGATGACGA+AGG + chr7_3:77052015-77052034 None:intergenic 55.0%
! GTCATCGCCGGTGCTTTTCT+CGG - chr7_3:77052017-77052036 Msa1125580:three_prime_UTR 55.0%
! CCGTTCACCGAGAAAAGCAC+CGG + chr7_3:77052027-77052046 None:intergenic 55.0%
! CCGGTGCTTTTCTCGGTGAA+CGG - chr7_3:77052024-77052043 Msa1125580:three_prime_UTR 55.0%
! CGGTGCTTTTCTCGGTGAAC+GGG - chr7_3:77052025-77052044 Msa1125580:three_prime_UTR 55.0%
GATACCGTGGCTGTCAACTG+CGG - chr7_3:77052461-77052480 Msa1125580:intron 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG - chr7_3:77052746-77052765 Msa1125580:intron 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG - chr7_3:77052747-77052766 Msa1125580:intron 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - chr7_3:77052748-77052767 Msa1125580:intron 55.0%
ACATTGGGAGGAGAGGGCTT+TGG - chr7_3:77052819-77052838 Msa1125580:intron 55.0%
!! GTATTGGGGGAGGGTTTTGG+GGG - chr7_3:77052899-77052918 Msa1125580:intron 55.0%
!!! TATTGGGGGAGGGTTTTGGG+GGG - chr7_3:77052900-77052919 Msa1125580:intron 55.0%
!!! AGGGTTTTGGGGGGTTATGG+AGG - chr7_3:77052909-77052928 Msa1125580:intron 55.0%
!!! GGGTTTTGGGGGGTTATGGA+GGG - chr7_3:77052910-77052929 Msa1125580:intron 55.0%
GAGTTTGAAGGGGAAGGGAG+GGG + chr7_3:77053099-77053118 None:intergenic 55.0%
GGAGTTTGAAGGGGAAGGGA+GGG + chr7_3:77053100-77053119 None:intergenic 55.0%
!! GGTTTGGGGGTGGATTGTAG+AGG - chr7_3:77053171-77053190 Msa1125580:intron 55.0%
!! TTGGAGGGGATGGCTTTGAG+GGG + chr7_3:77053408-77053427 None:intergenic 55.0%
CGAGTTTGGAGGGGAAGAGA+GGG + chr7_3:77053457-77053476 None:intergenic 55.0%
GGCTCTGTTTGCGAGTTTGG+AGG + chr7_3:77053468-77053487 None:intergenic 55.0%
GGAAACAGGCAGTGTCCGGT+CGG - chr7_3:77051759-77051778 Msa1125580:three_prime_UTR 60.0%
! TTGGGAGGAGAGGGCTTTGG+AGG - chr7_3:77052822-77052841 Msa1125580:intron 60.0%
!!! GGGAGGGTTTTGGGGGGTTA+TGG - chr7_3:77052906-77052925 Msa1125580:intron 60.0%
AAGGGAGGGGAGGGATTTGG+GGG + chr7_3:77053086-77053105 None:intergenic 60.0%
GAAGGGAGGGGAGGGATTTG+GGG + chr7_3:77053087-77053106 None:intergenic 60.0%
GGAAGGGAGGGGAGGGATTT+GGG + chr7_3:77053088-77053107 None:intergenic 60.0%
TTGAAGGGGAAGGGAGGGGA+GGG + chr7_3:77053095-77053114 None:intergenic 60.0%
TTTGAAGGGGAAGGGAGGGG+AGG + chr7_3:77053096-77053115 None:intergenic 60.0%
GGGAGTTTGAAGGGGAAGGG+AGG + chr7_3:77053101-77053120 None:intergenic 60.0%
GGAGGGGAAGAGAGGGCTTT+AGG + chr7_3:77053450-77053469 None:intergenic 60.0%
GCGAGTTTGGAGGGGAAGAG+AGG + chr7_3:77053458-77053477 None:intergenic 60.0%
TTCTGTGCTGGGGCAAAGGC+CGG - chr7_3:77054293-77054312 Msa1125580:CDS 60.0%
AGTGTCCGGTCGGATCGAGC+CGG - chr7_3:77051769-77051788 Msa1125580:three_prime_UTR 65.0%
GTGGCCGCAGTTGACAGCCA+CGG + chr7_3:77052468-77052487 None:intergenic 65.0%
GGAGTTTGGAGGGGAGGGGA+GGG - chr7_3:77052752-77052771 Msa1125580:intron 65.0%
GAGTTTGGAGGGGAGGGGAG+GGG - chr7_3:77052753-77052772 Msa1125580:intron 65.0%
! GGGAGGGGAGGGGAGAGTTT+TGG - chr7_3:77052763-77052782 Msa1125580:intron 65.0%
AGGGGAGGGATTTGGGGGGT+TGG + chr7_3:77053081-77053100 None:intergenic 65.0%
AGGGAGGGGAGGGATTTGGG+GGG + chr7_3:77053085-77053104 None:intergenic 65.0%
GGGAAGGGAGGGGAGGGATT+TGG + chr7_3:77053089-77053108 None:intergenic 65.0%
GCAAGTTTGGAGGGGGAGGG+AGG - chr7_3:77053144-77053163 Msa1125580:intron 65.0%
!! CCGTACCGGCTCGATCCGAC+CGG + chr7_3:77051777-77051796 None:intergenic 70.0%
CCGGTCGGATCGAGCCGGTA+CGG - chr7_3:77051774-77051793 Msa1125580:three_prime_UTR 70.0%
GGGAGTTTGGAGGGGAGGGG+AGG - chr7_3:77052751-77052770 Msa1125580:intron 70.0%
TTTGGAGGGGGAGGGAGGCG+AGG - chr7_3:77053149-77053168 Msa1125580:intron 70.0%
TTGGAGGGGGAGGGAGGCGA+GGG - chr7_3:77053150-77053169 Msa1125580:intron 70.0%
GGGGAGGGAGGCGAGGGTTT+GGG - chr7_3:77053156-77053175 Msa1125580:intron 70.0%
GGGAGGGAGGCGAGGGTTTG+GGG - chr7_3:77053157-77053176 Msa1125580:intron 70.0%
GGAGGGAGGCGAGGGTTTGG+GGG - chr7_3:77053158-77053177 Msa1125580:intron 70.0%
GGGGGAGGGAGGCGAGGGTT+TGG - chr7_3:77053155-77053174 Msa1125580:intron 75.0%
GGGAGGCGAGGGTTTGGGGG+TGG - chr7_3:77053161-77053180 Msa1125580:intron 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 77051750 77054715 77051750 ID=Msa1125580;Name=Msa1125580
chr7_3 mRNA 77051750 77054715 77051750 ID=Msa1125580-mRNA-1;Parent=Msa1125580;Name=Msa1125580-mRNA-1;_AED=0.09;_eAED=0.09;_QI=177|1|1|1|1|1|3|389|72
chr7_3 exon 77051750 77052189 77051750 ID=Msa1125580-mRNA-1:exon:21185;Parent=Msa1125580-mRNA-1
chr7_3 exon 77054289 77054336 77054289 ID=Msa1125580-mRNA-1:exon:21184;Parent=Msa1125580-mRNA-1
chr7_3 exon 77054419 77054715 77054419 ID=Msa1125580-mRNA-1:exon:21183;Parent=Msa1125580-mRNA-1
chr7_3 five_prime_UTR 77054539 77054715 77054539 ID=Msa1125580-mRNA-1:five_prime_utr;Parent=Msa1125580-mRNA-1
chr7_3 CDS 77054419 77054538 77054419 ID=Msa1125580-mRNA-1:cds;Parent=Msa1125580-mRNA-1
chr7_3 CDS 77054289 77054336 77054289 ID=Msa1125580-mRNA-1:cds;Parent=Msa1125580-mRNA-1
chr7_3 CDS 77052139 77052189 77052139 ID=Msa1125580-mRNA-1:cds;Parent=Msa1125580-mRNA-1
chr7_3 three_prime_UTR 77051750 77052138 77051750 ID=Msa1125580-mRNA-1:three_prime_utr;Parent=Msa1125580-mRNA-1
Gene Sequence

>Msa1125580

ATGGAATCCGCTGCTAAAAGAAGAGGAGGAGGCGTTTTGGAAGGCCTTTACAAGGTTCTTATGCGCCGCAACTCTGTCTATGTTACCTTCGTCATCGCCGGTGCTTTTCTCGGTGAACGGGCTGTGGATTACGGTGTTCACAAGCTCTGGGAAATAAATAATGTTGGGAAACGATATGAAGACATTTCTGTGCTGGGGCAAAGGCCGGTAGACGAGTGA
Protein sequence

>Msa1125580

MESAAKRRGGGVLEGLYKVLMRRNSVYVTFVIAGAFLGERAVDYGVHKLWEINNVGKRYEDISVLGQRPVDE*