AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1182420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 104 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTAATTCTATATGTAATT+TGG 0.113702 8_1:+17528929 Msa1182420:three_prime_UTR
TCTTGAAATAATAGAAAATT+TGG 0.114067 8_1:-17527331 None:intergenic
TCCATCCATGTTGCATTAAT+TGG 0.147680 8_1:-17528809 None:intergenic
TGAGGAACTCAAACAATTTA+AGG 0.163299 8_1:+17527969 Msa1182420:CDS
GAATTTCAAATTCGAATTTA+TGG 0.182017 8_1:+17527537 Msa1182420:five_prime_UTR
CCGTTTAGGTTAAATTCTTA+TGG 0.198762 8_1:+17527072 Msa1182420:five_prime_UTR
GTCCACAATCACATTTAAAT+TGG 0.209735 8_1:-17528522 None:intergenic
ATTATATATATCAATGTATT+TGG 0.214681 8_1:+17529023 Msa1182420:three_prime_UTR
GGGTGAATTTAGGACTAAAT+AGG 0.238968 8_1:+17527716 Msa1182420:five_prime_UTR
GTGAACTCCTTGAACATTTC+CGG 0.239784 8_1:-17526647 None:intergenic
TTTGGATTGCCTTTGTCATT+AGG 0.280539 8_1:+17527140 Msa1182420:five_prime_UTR
CTGAACATACTCATAATAAA+AGG 0.293763 8_1:-17526702 None:intergenic
GCTTACGAGAGGGTGAATTT+AGG 0.294219 8_1:+17527706 Msa1182420:five_prime_UTR
ATTAGGACTTAGGTTGTTAC+AGG 0.300196 8_1:+17527157 Msa1182420:five_prime_UTR
GGTGAATTTAGGACTAAATA+GGG 0.309034 8_1:+17527717 Msa1182420:five_prime_UTR
GCAGTAAAGTCCAGCATAAA+AGG 0.309456 8_1:-17527356 None:intergenic
TGCTATTAGTATTTCTATCT+TGG 0.314840 8_1:+17526726 Msa1182420:five_prime_UTR
TCACATTTAAATTGGCCATT+TGG 0.332786 8_1:-17528514 None:intergenic
CTCCAACAATGTCAAATTGC+AGG 0.336874 8_1:+17527874 Msa1182420:five_prime_UTR
GACCAAGCATTTGACATTGT+TGG 0.343423 8_1:-17527913 None:intergenic
ATAGTACTAGATTGCCGTTT+AGG 0.345751 8_1:+17527058 Msa1182420:five_prime_UTR
AACTCTGAAAATTGTAGGCT+TGG 0.362647 8_1:+17527842 Msa1182420:five_prime_UTR
CAATGTCAAATTGCAGGCTT+GGG 0.366731 8_1:+17527880 Msa1182420:five_prime_UTR
ATGGTTGAAGTTGCTATCAT+TGG 0.374901 8_1:+17526928 Msa1182420:five_prime_UTR
TGAAAACTTTCTTCTGATAC+AGG 0.377739 8_1:-17527564 None:intergenic
CATAGTGACAATTACAGAAT+TGG 0.378131 8_1:-17528861 None:intergenic
GTGATTTCATTTATGAATGA+TGG 0.382008 8_1:+17526748 Msa1182420:five_prime_UTR
ACTCTGAAAATTGTAGGCTT+GGG 0.385989 8_1:+17527843 Msa1182420:five_prime_UTR
CCATAAGAATTTAACCTAAA+CGG 0.392976 8_1:-17527072 None:intergenic
ACAAAACTGACATAATAGTT+TGG 0.402172 8_1:+17526837 Msa1182420:five_prime_UTR
ACAATGTCAAATGCTTGGTC+TGG 0.403543 8_1:+17527916 Msa1182420:exon
AGCCTGCAATTTGACATTGT+TGG 0.403841 8_1:-17527876 None:intergenic
TTAGAAGCAGGTTAATTTAG+TGG 0.406686 8_1:-17526957 None:intergenic
CAAATAAAACATATTTCCTA+TGG 0.409330 8_1:+17526995 Msa1182420:five_prime_UTR
ACAATGTCAAATTGCAGGCT+TGG 0.414903 8_1:+17527879 Msa1182420:five_prime_UTR
TTCCTCAAGTCCCTCTAGTT+AGG 0.416195 8_1:-17527517 None:intergenic
TGCCTTTGTCATTAGGACTT+AGG 0.438900 8_1:+17527147 Msa1182420:five_prime_UTR
TTATGGAACGACAATAGATA+TGG 0.442703 8_1:+17527089 Msa1182420:five_prime_UTR
GCATCATTTGAAGGATTTGA+AGG 0.458553 8_1:-17527448 None:intergenic
TGACATAATAGTTTGGAACT+TGG 0.463445 8_1:+17526844 Msa1182420:five_prime_UTR
AGGTACAAATTTCCAATTGT+AGG 0.463626 8_1:+17528664 Msa1182420:intron
ATTAACCTGCTTCTAATGCT+TGG 0.464093 8_1:+17526964 Msa1182420:five_prime_UTR
AAGGGAGGAAAGTGGCATAA+TGG 0.466814 8_1:+17526909 Msa1182420:five_prime_UTR
AACAACATGGCATCATTTGA+AGG 0.470632 8_1:-17527457 None:intergenic
AAATTGTTTGAGTTCCTCAA+TGG 0.471669 8_1:-17527965 None:intergenic
CTCCAACAATGTCAAATGCT+TGG 0.473618 8_1:+17527911 Msa1182420:exon
GCTAAAATTAAGCCACCCTC+AGG 0.476976 8_1:+17527771 Msa1182420:five_prime_UTR
TAGTGGTTAATGAAATCTGA+TGG 0.484600 8_1:+17528746 Msa1182420:three_prime_UTR
CAATGTCAAATGCTTGGTCT+GGG 0.486270 8_1:+17527917 Msa1182420:exon
GGCAATCTAGTACTATAAAA+CGG 0.489299 8_1:-17527051 None:intergenic
ATGATTTGATAAAATTAACT+TGG 0.492727 8_1:-17528778 None:intergenic
ATGAGAAAGAACATAAATAG+TGG 0.495321 8_1:+17528729 Msa1182420:three_prime_UTR
GACATAATAGTTTGGAACTT+GGG 0.496622 8_1:+17526845 Msa1182420:five_prime_UTR
CAAGTCCCTCTAGCGAGGCT+TGG 0.500252 8_1:-17527628 None:intergenic
AATTTGACATTGTTGGAGAA+TGG 0.515371 8_1:-17527869 None:intergenic
TGAACATTTCCGGTGCACTA+AGG 0.516804 8_1:-17526637 None:intergenic
AGGAAGGCTAAAACTCCAAA+TGG 0.517189 8_1:+17528499 Msa1182420:CDS
TAGTGCACCGGAAATGTTCA+AGG 0.520766 8_1:+17526640 Msa1182420:five_prime_UTR
TCCAATTAATGCAACATGGA+TGG 0.520892 8_1:+17528808 Msa1182420:three_prime_UTR
CTATAAAACGGCTGAGACAG+TGG 0.523339 8_1:-17527039 None:intergenic
AAAAGTGTATAATAGTAAGT+AGG 0.524753 8_1:-17527262 None:intergenic
CATTTGACATTGTTGGAGCA+TGG 0.525905 8_1:-17527906 None:intergenic
TAATGCAACATGGATGGACT+TGG 0.527685 8_1:+17528814 Msa1182420:three_prime_UTR
TATTAATGATGATTCCATTG+AGG 0.530317 8_1:+17527951 Msa1182420:CDS
AGGGTGAATTTCACATGAAG+TGG 0.535164 8_1:+17527601 Msa1182420:five_prime_UTR
TGTAAAATTGTTCTACGGAA+GGG 0.540161 8_1:+17526891 Msa1182420:five_prime_UTR
GGCCAATTTAAATGTGATTG+TGG 0.541689 8_1:+17528520 Msa1182420:CDS
TTTCATTGGTACTTATAAGA+GGG 0.541716 8_1:+17527582 Msa1182420:five_prime_UTR
CTCTGAAAATTGTAGGCTTG+GGG 0.549249 8_1:+17527844 Msa1182420:five_prime_UTR
ATGTAAAATTGTTCTACGGA+AGG 0.564499 8_1:+17526890 Msa1182420:five_prime_UTR
ATATATGTAAAATTGTTCTA+CGG 0.564873 8_1:+17526886 Msa1182420:five_prime_UTR
AGTGAAAGGGTTGAATCCAT+AGG 0.568408 8_1:-17527011 None:intergenic
AGACAGTGGGATACAGTGAA+AGG 0.571434 8_1:-17527025 None:intergenic
AACTCAAACAATTTAAGGAA+AGG 0.573878 8_1:+17527974 Msa1182420:CDS
TCTCACCAAGCATTAGAAGC+AGG 0.582699 8_1:-17526969 None:intergenic
GCAGAGCAACCTTAGTGCAC+CGG 0.586537 8_1:+17526628 Msa1182420:intron
GACAGTGGGATACAGTGAAA+GGG 0.586955 8_1:-17527024 None:intergenic
GTGAATTTAGGACTAAATAG+GGG 0.588267 8_1:+17527718 Msa1182420:five_prime_UTR
TCATCAACATTAGCATCACC+TGG 0.591889 8_1:+17525996 Msa1182420:five_prime_UTR
TTATTGCACGTCACATGCAG+TGG 0.592615 8_1:+17527485 Msa1182420:five_prime_UTR
GAGAACCAAGCCTCGCTAGA+GGG 0.603704 8_1:+17527623 Msa1182420:five_prime_UTR
AGTGGTTAATGAAATCTGAT+GGG 0.604425 8_1:+17528747 Msa1182420:three_prime_UTR
GTTTATACTAAACCTGAGGG+TGG 0.620466 8_1:-17527783 None:intergenic
AATAGTTTATACTAAACCTG+AGG 0.630732 8_1:-17527787 None:intergenic
ACTCTCCAATTAATGCAACA+TGG 0.635926 8_1:+17528804 Msa1182420:three_prime_UTR
CTCCTCAAGTCCCTCTAGCG+AGG 0.636119 8_1:-17527633 None:intergenic
GGAGAACCAAGCCTCGCTAG+AGG 0.636432 8_1:+17527622 Msa1182420:five_prime_UTR
TTCTACGGAAGGGAGGAAAG+TGG 0.638672 8_1:+17526901 Msa1182420:five_prime_UTR
AATGTCAAATTGCAGGCTTG+GGG 0.640848 8_1:+17527881 Msa1182420:five_prime_UTR
AGCCTAACTAGAGGGACTTG+AGG 0.643226 8_1:+17527515 Msa1182420:five_prime_UTR
ATAGTTTATACTAAACCTGA+GGG 0.645080 8_1:-17527786 None:intergenic
AAAATTGTTCTACGGAAGGG+AGG 0.645464 8_1:+17526894 Msa1182420:five_prime_UTR
AGCCTCGCTAGAGGGACTTG+AGG 0.646190 8_1:+17527631 Msa1182420:five_prime_UTR
TATAAAACGGCTGAGACAGT+GGG 0.646824 8_1:-17527038 None:intergenic
AACCTAAGTCCTAATGACAA+AGG 0.648103 8_1:-17527149 None:intergenic
GAGAACTAAGCCTAACTAGA+GGG 0.649306 8_1:+17527507 Msa1182420:five_prime_UTR
ACAGCTGCACTAAATGCACC+AGG 0.652832 8_1:+17528479 Msa1182420:intron
CACTTCTGTCAATGAGACTG+TGG 0.662055 8_1:+17528838 Msa1182420:three_prime_UTR
TTAGGACTTAGGTTGTTACA+GGG 0.664202 8_1:+17527158 Msa1182420:five_prime_UTR
AGGTGATGCTAATGTTGATG+AGG 0.665671 8_1:-17525994 None:intergenic
GGAGAACTAAGCCTAACTAG+AGG 0.665818 8_1:+17527506 Msa1182420:five_prime_UTR
CTGCACTAAATGCACCAGGA+AGG 0.665837 8_1:+17528483 Msa1182420:CDS
AAATGTTCAAGGAGTTCACA+TGG 0.681365 8_1:+17526651 Msa1182420:five_prime_UTR
ACAGGGTATGAAATATAACA+AGG 0.694952 8_1:+17527175 Msa1182420:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCATGAATAAAAAAAAA+AGG - chr8_1:17526034-17526053 None:intergenic 10.0%
!!! AAAATTGATTTATGTTTAAT+TGG - chr8_1:17528003-17528022 None:intergenic 10.0%
!! ATTATATATATCAATGTATT+TGG + chr8_1:17529023-17529042 Msa1182420:three_prime_UTR 10.0%
!! ATGAATAAAAAAAAAAGGAA+GGG - chr8_1:17526029-17526048 None:intergenic 15.0%
!!! TTTTTATATATACATGTCAT+CGG + chr8_1:17526419-17526438 Msa1182420:intron 15.0%
!! ATATATGTAAAATTGTTCTA+CGG + chr8_1:17526886-17526905 Msa1182420:five_prime_UTR 15.0%
!!! CTTTATACAATATGAATTTT+TGG + chr8_1:17527122-17527141 Msa1182420:five_prime_UTR 15.0%
!!! TACAAACTTTTTATTAAAGT+AGG - chr8_1:17527227-17527246 None:intergenic 15.0%
!! TCTTGAAATAATAGAAAATT+TGG - chr8_1:17527334-17527353 None:intergenic 15.0%
!! AATAAAAACTTTCATCTATT+AGG - chr8_1:17527683-17527702 None:intergenic 15.0%
!!! TAATAGATGAAAGTTTTTAT+TGG + chr8_1:17527682-17527701 Msa1182420:five_prime_UTR 15.0%
!!! CTTTTTATTTATAGCTTTTT+GGG + chr8_1:17528177-17528196 Msa1182420:intron 15.0%
!! CTAAATTAATAATAGAGAAT+TGG - chr8_1:17528238-17528257 None:intergenic 15.0%
!! CTGTTATTATTATTATTACA+AGG + chr8_1:17528377-17528396 Msa1182420:intron 15.0%
!! ATGATTTGATAAAATTAACT+TGG - chr8_1:17528781-17528800 None:intergenic 15.0%
!! TACTAATTCTATATGTAATT+TGG + chr8_1:17528929-17528948 Msa1182420:three_prime_UTR 15.0%
!!! TTTTTTATTGATAAACTCAT+TGG + chr8_1:17528979-17528998 Msa1182420:three_prime_UTR 15.0%
!! CATGAATAAAAAAAAAAGGA+AGG - chr8_1:17526030-17526049 None:intergenic 20.0%
!! TCTGATTAAAACAATAAAGA+AGG - chr8_1:17526306-17526325 None:intergenic 20.0%
!!! TATTTCTAAGTTTTCTCAAA+AGG + chr8_1:17526334-17526353 Msa1182420:intron 20.0%
!! TACAAATATTTGCTTTACTT+TGG - chr8_1:17526387-17526406 None:intergenic 20.0%
!! TAAAGCAAATATTTGTAGTA+TGG + chr8_1:17526390-17526409 Msa1182420:intron 20.0%
!! AACGATGTAAATCAAAATAA+AGG - chr8_1:17526479-17526498 None:intergenic 20.0%
!!! TATTTTGATTTACATCGTTA+TGG + chr8_1:17526480-17526499 Msa1182420:intron 20.0%
!! CATATTATAAAAAAACATGC+AGG - chr8_1:17526609-17526628 None:intergenic 20.0%
!! CAAATAAAACATATTTCCTA+TGG + chr8_1:17526995-17527014 Msa1182420:five_prime_UTR 20.0%
!!! AAAAGTGTATAATAGTAAGT+AGG - chr8_1:17527265-17527284 None:intergenic 20.0%
!! GAATTTCAAATTCGAATTTA+TGG + chr8_1:17527537-17527556 Msa1182420:five_prime_UTR 20.0%
!!! ACAATTTTCAGAGTTTTTAA+TGG - chr8_1:17527837-17527856 None:intergenic 20.0%
!!! TTAAAAACTCTGAAAATTGT+AGG + chr8_1:17527837-17527856 Msa1182420:five_prime_UTR 20.0%
!! CCAAAAAGCTATAAATAAAA+AGG - chr8_1:17528179-17528198 None:intergenic 20.0%
!!! CCTTTTTATTTATAGCTTTT+TGG + chr8_1:17528176-17528195 Msa1182420:intron 20.0%
!!! CTTTTTACTTGTTCAATTTA+TGG - chr8_1:17528324-17528343 None:intergenic 20.0%
!!! CATGTATATTTTTTTACCTT+GGG - chr8_1:17528567-17528586 None:intergenic 20.0%
!!! ACATGTATATTTTTTTACCT+TGG - chr8_1:17528568-17528587 None:intergenic 20.0%
! TCAGATTTATTTGAAAAGAG+AGG + chr8_1:17526165-17526184 Msa1182420:intron 25.0%
! CAGATTTATTTGAAAAGAGA+GGG + chr8_1:17526166-17526185 Msa1182420:intron 25.0%
! CTGAACATACTCATAATAAA+AGG - chr8_1:17526705-17526724 None:intergenic 25.0%
!! TGCTATTAGTATTTCTATCT+TGG + chr8_1:17526726-17526745 Msa1182420:five_prime_UTR 25.0%
! GTGATTTCATTTATGAATGA+TGG + chr8_1:17526748-17526767 Msa1182420:five_prime_UTR 25.0%
! ACAAAACTGACATAATAGTT+TGG + chr8_1:17526837-17526856 Msa1182420:five_prime_UTR 25.0%
! CCATAAGAATTTAACCTAAA+CGG - chr8_1:17527075-17527094 None:intergenic 25.0%
!! CATATTTGTGAATTTTGAAC+TGG - chr8_1:17527397-17527416 None:intergenic 25.0%
! TGCAATAAAAAACAACAACA+TGG - chr8_1:17527473-17527492 None:intergenic 25.0%
!! TATCAGAAGAAAGTTTTCAT+TGG + chr8_1:17527568-17527587 Msa1182420:five_prime_UTR 25.0%
!! TTTTCATTGGTACTTATAAG+AGG + chr8_1:17527581-17527600 Msa1182420:five_prime_UTR 25.0%
! TTTCATTGGTACTTATAAGA+GGG + chr8_1:17527582-17527601 Msa1182420:five_prime_UTR 25.0%
! ATAGTTTATACTAAACCTGA+GGG - chr8_1:17527789-17527808 None:intergenic 25.0%
! AATAGTTTATACTAAACCTG+AGG - chr8_1:17527790-17527809 None:intergenic 25.0%
!! GAGTTTTTAATGGTTGTAAA+TGG - chr8_1:17527827-17527846 None:intergenic 25.0%
! TATTAATGATGATTCCATTG+AGG + chr8_1:17527951-17527970 Msa1182420:CDS 25.0%
! AACTCAAACAATTTAAGGAA+AGG + chr8_1:17527974-17527993 Msa1182420:CDS 25.0%
! AGACTTAGAATTGTGTTAAT+TGG + chr8_1:17528285-17528304 Msa1182420:intron 25.0%
! TTGATAAAAAGACCTACAAT+TGG - chr8_1:17528679-17528698 None:intergenic 25.0%
! ATGAGAAAGAACATAAATAG+TGG + chr8_1:17528729-17528748 Msa1182420:three_prime_UTR 25.0%
!! GTAATTGTCACTATGTTTTA+AGG + chr8_1:17528869-17528888 Msa1182420:three_prime_UTR 25.0%
!!! TATGTTTTAAGGACTAACTT+GGG + chr8_1:17528880-17528899 Msa1182420:three_prime_UTR 25.0%
CTTGCATGTTAGTTGAAATT+AGG - chr8_1:17526201-17526220 None:intergenic 30.0%
! AAGGAGTTGAAATCTTTTCA+TGG + chr8_1:17526353-17526372 Msa1182420:intron 30.0%
! AGGAGTTGAAATCTTTTCAT+GGG + chr8_1:17526354-17526373 Msa1182420:intron 30.0%
! TGACATAATAGTTTGGAACT+TGG + chr8_1:17526844-17526863 Msa1182420:five_prime_UTR 30.0%
! GACATAATAGTTTGGAACTT+GGG + chr8_1:17526845-17526864 Msa1182420:five_prime_UTR 30.0%
ATGTAAAATTGTTCTACGGA+AGG + chr8_1:17526890-17526909 Msa1182420:five_prime_UTR 30.0%
TGTAAAATTGTTCTACGGAA+GGG + chr8_1:17526891-17526910 Msa1182420:five_prime_UTR 30.0%
TTAGAAGCAGGTTAATTTAG+TGG - chr8_1:17526960-17526979 None:intergenic 30.0%
GGCAATCTAGTACTATAAAA+CGG - chr8_1:17527054-17527073 None:intergenic 30.0%
! CCGTTTAGGTTAAATTCTTA+TGG + chr8_1:17527072-17527091 Msa1182420:five_prime_UTR 30.0%
TTATGGAACGACAATAGATA+TGG + chr8_1:17527089-17527108 Msa1182420:five_prime_UTR 30.0%
ACAGGGTATGAAATATAACA+AGG + chr8_1:17527175-17527194 Msa1182420:five_prime_UTR 30.0%
! TTTCAAGAATCCTTTTATGC+TGG + chr8_1:17527346-17527365 Msa1182420:five_prime_UTR 30.0%
TGAAAACTTTCTTCTGATAC+AGG - chr8_1:17527567-17527586 None:intergenic 30.0%
! GGTGAATTTAGGACTAAATA+GGG + chr8_1:17527717-17527736 Msa1182420:five_prime_UTR 30.0%
! GTGAATTTAGGACTAAATAG+GGG + chr8_1:17527718-17527737 Msa1182420:five_prime_UTR 30.0%
AATTTGACATTGTTGGAGAA+TGG - chr8_1:17527872-17527891 None:intergenic 30.0%
! AAATTGTTTGAGTTCCTCAA+TGG - chr8_1:17527968-17527987 None:intergenic 30.0%
TGAGGAACTCAAACAATTTA+AGG + chr8_1:17527969-17527988 Msa1182420:CDS 30.0%
! ACAAGCATCACAATTTTCAA+AGG + chr8_1:17528084-17528103 Msa1182420:intron 30.0%
CAAACAATAGCTTCAATAAC+AGG - chr8_1:17528113-17528132 None:intergenic 30.0%
TTGAAGCTATTGTTTGTGAA+AGG + chr8_1:17528117-17528136 Msa1182420:intron 30.0%
TCACATTTAAATTGGCCATT+TGG - chr8_1:17528517-17528536 None:intergenic 30.0%
GTCCACAATCACATTTAAAT+TGG - chr8_1:17528525-17528544 None:intergenic 30.0%
TATACATGTCTTTGTGCTTA+TGG + chr8_1:17528579-17528598 Msa1182420:intron 30.0%
! ACTTGATTCGATAATTGACA+TGG + chr8_1:17528624-17528643 Msa1182420:intron 30.0%
AGGTACAAATTTCCAATTGT+AGG + chr8_1:17528664-17528683 Msa1182420:intron 30.0%
! TGTAGGTCTTTTTATCAAGT+AGG + chr8_1:17528681-17528700 Msa1182420:CDS 30.0%
TAGTGGTTAATGAAATCTGA+TGG + chr8_1:17528746-17528765 Msa1182420:three_prime_UTR 30.0%
AGTGGTTAATGAAATCTGAT+GGG + chr8_1:17528747-17528766 Msa1182420:three_prime_UTR 30.0%
CATAGTGACAATTACAGAAT+TGG - chr8_1:17528864-17528883 None:intergenic 30.0%
!!! CTATGTTTTAAGGACTAACT+TGG + chr8_1:17528879-17528898 Msa1182420:three_prime_UTR 30.0%
ATTTACCAATCTATGTGTGC+AGG - chr8_1:17526114-17526133 None:intergenic 35.0%
AAGCAATAAGCAGATATGTG+TGG - chr8_1:17526276-17526295 None:intergenic 35.0%
TTTACATCGTTATGGACCTA+TGG + chr8_1:17526488-17526507 Msa1182420:intron 35.0%
AAATGTTCAAGGAGTTCACA+TGG + chr8_1:17526651-17526670 Msa1182420:five_prime_UTR 35.0%
ATGGTTGAAGTTGCTATCAT+TGG + chr8_1:17526928-17526947 Msa1182420:five_prime_UTR 35.0%
ATTAACCTGCTTCTAATGCT+TGG + chr8_1:17526964-17526983 Msa1182420:five_prime_UTR 35.0%
! ATAGTACTAGATTGCCGTTT+AGG + chr8_1:17527058-17527077 Msa1182420:five_prime_UTR 35.0%
TTTGGATTGCCTTTGTCATT+AGG + chr8_1:17527140-17527159 Msa1182420:five_prime_UTR 35.0%
AACCTAAGTCCTAATGACAA+AGG - chr8_1:17527152-17527171 None:intergenic 35.0%
! ATTAGGACTTAGGTTGTTAC+AGG + chr8_1:17527157-17527176 Msa1182420:five_prime_UTR 35.0%
! TTAGGACTTAGGTTGTTACA+GGG + chr8_1:17527158-17527177 Msa1182420:five_prime_UTR 35.0%
! GAATTTTGAACTGGCAGAAA+GGG - chr8_1:17527388-17527407 None:intergenic 35.0%
! TGAATTTTGAACTGGCAGAA+AGG - chr8_1:17527389-17527408 None:intergenic 35.0%
GCATCATTTGAAGGATTTGA+AGG - chr8_1:17527451-17527470 None:intergenic 35.0%
AACAACATGGCATCATTTGA+AGG - chr8_1:17527460-17527479 None:intergenic 35.0%
!! TTTTTATTGGTGCTTACGAG+AGG + chr8_1:17527695-17527714 Msa1182420:five_prime_UTR 35.0%
!! TTTTATTGGTGCTTACGAGA+GGG + chr8_1:17527696-17527715 Msa1182420:five_prime_UTR 35.0%
! GGGTGAATTTAGGACTAAAT+AGG + chr8_1:17527716-17527735 Msa1182420:five_prime_UTR 35.0%
! AACTCTGAAAATTGTAGGCT+TGG + chr8_1:17527842-17527861 Msa1182420:five_prime_UTR 35.0%
! ACTCTGAAAATTGTAGGCTT+GGG + chr8_1:17527843-17527862 Msa1182420:five_prime_UTR 35.0%
CATCATGACTGAACTTTACA+AGG + chr8_1:17528435-17528454 Msa1182420:intron 35.0%
GAACTTTACAAGGTCATTCA+CGG + chr8_1:17528445-17528464 Msa1182420:intron 35.0%
GGCCAATTTAAATGTGATTG+TGG + chr8_1:17528520-17528539 Msa1182420:CDS 35.0%
! TTGTGGACAAGTTTTTGAAC+AGG + chr8_1:17528537-17528556 Msa1182420:CDS 35.0%
TGTGCTTATGGATATCATGA+TGG + chr8_1:17528591-17528610 Msa1182420:intron 35.0%
! TGGTAGTTTCTTTTGTTCAC+AGG + chr8_1:17528644-17528663 Msa1182420:intron 35.0%
ACTCTCCAATTAATGCAACA+TGG + chr8_1:17528804-17528823 Msa1182420:three_prime_UTR 35.0%
TCCAATTAATGCAACATGGA+TGG + chr8_1:17528808-17528827 Msa1182420:three_prime_UTR 35.0%
TCCATCCATGTTGCATTAAT+TGG - chr8_1:17528812-17528831 None:intergenic 35.0%
!! GGAATAATCGAAGCATTTTC+TGG + chr8_1:17529000-17529019 Msa1182420:three_prime_UTR 35.0%
AGGTGATGCTAATGTTGATG+AGG - chr8_1:17525997-17526016 None:intergenic 40.0%
TCATCAACATTAGCATCACC+TGG + chr8_1:17525996-17526015 Msa1182420:five_prime_UTR 40.0%
CAATCTATGTGTGCAGGATA+CGG - chr8_1:17526108-17526127 None:intergenic 40.0%
GTGAACTCCTTGAACATTTC+CGG - chr8_1:17526650-17526669 None:intergenic 40.0%
AAAATTGTTCTACGGAAGGG+AGG + chr8_1:17526894-17526913 Msa1182420:five_prime_UTR 40.0%
AGTGAAAGGGTTGAATCCAT+AGG - chr8_1:17527014-17527033 None:intergenic 40.0%
TATAAAACGGCTGAGACAGT+GGG - chr8_1:17527041-17527060 None:intergenic 40.0%
TGCCTTTGTCATTAGGACTT+AGG + chr8_1:17527147-17527166 Msa1182420:five_prime_UTR 40.0%
GCAGTAAAGTCCAGCATAAA+AGG - chr8_1:17527359-17527378 None:intergenic 40.0%
GAGAACTAAGCCTAACTAGA+GGG + chr8_1:17527507-17527526 Msa1182420:five_prime_UTR 40.0%
AGGGTGAATTTCACATGAAG+TGG + chr8_1:17527601-17527620 Msa1182420:five_prime_UTR 40.0%
GTTTATACTAAACCTGAGGG+TGG - chr8_1:17527786-17527805 None:intergenic 40.0%
! CTCTGAAAATTGTAGGCTTG+GGG + chr8_1:17527844-17527863 Msa1182420:five_prime_UTR 40.0%
CTCCAACAATGTCAAATTGC+AGG + chr8_1:17527874-17527893 Msa1182420:five_prime_UTR 40.0%
AGCCTGCAATTTGACATTGT+TGG - chr8_1:17527879-17527898 None:intergenic 40.0%
ACAATGTCAAATTGCAGGCT+TGG + chr8_1:17527879-17527898 Msa1182420:five_prime_UTR 40.0%
CAATGTCAAATTGCAGGCTT+GGG + chr8_1:17527880-17527899 Msa1182420:five_prime_UTR 40.0%
AATGTCAAATTGCAGGCTTG+GGG + chr8_1:17527881-17527900 Msa1182420:five_prime_UTR 40.0%
CATTTGACATTGTTGGAGCA+TGG - chr8_1:17527909-17527928 None:intergenic 40.0%
CTCCAACAATGTCAAATGCT+TGG + chr8_1:17527911-17527930 Msa1182420:exon 40.0%
GACCAAGCATTTGACATTGT+TGG - chr8_1:17527916-17527935 None:intergenic 40.0%
ACAATGTCAAATGCTTGGTC+TGG + chr8_1:17527916-17527935 Msa1182420:exon 40.0%
CAATGTCAAATGCTTGGTCT+GGG + chr8_1:17527917-17527936 Msa1182420:exon 40.0%
AGCTATTGTTTGTGAAAGGC+AGG + chr8_1:17528121-17528140 Msa1182420:intron 40.0%
!! TCAGAGTGTGATTTTCAAGC+AGG + chr8_1:17528212-17528231 Msa1182420:intron 40.0%
TTTACAAGGTCATTCACGGT+TGG + chr8_1:17528449-17528468 Msa1182420:intron 40.0%
!! ATTTGGAGTTTTAGCCTTCC+TGG - chr8_1:17528500-17528519 None:intergenic 40.0%
AGGAAGGCTAAAACTCCAAA+TGG + chr8_1:17528499-17528518 Msa1182420:CDS 40.0%
! TTTTTGAACAGGCAATCCCA+AGG + chr8_1:17528548-17528567 Msa1182420:CDS 40.0%
! TAATGCAACATGGATGGACT+TGG + chr8_1:17528814-17528833 Msa1182420:three_prime_UTR 40.0%
!! AAAAGGAAGGGCATCAAACC+AGG - chr8_1:17526017-17526036 None:intergenic 45.0%
CGTATCCTGCACACATAGAT+TGG + chr8_1:17526106-17526125 Msa1182420:intron 45.0%
CTAGTCGTCTACATCACCAT+AGG - chr8_1:17526507-17526526 None:intergenic 45.0%
TGAACATTTCCGGTGCACTA+AGG - chr8_1:17526640-17526659 None:intergenic 45.0%
TAGTGCACCGGAAATGTTCA+AGG + chr8_1:17526640-17526659 Msa1182420:five_prime_UTR 45.0%
AAGGGAGGAAAGTGGCATAA+TGG + chr8_1:17526909-17526928 Msa1182420:five_prime_UTR 45.0%
TCTCACCAAGCATTAGAAGC+AGG - chr8_1:17526972-17526991 None:intergenic 45.0%
GACAGTGGGATACAGTGAAA+GGG - chr8_1:17527027-17527046 None:intergenic 45.0%
AGACAGTGGGATACAGTGAA+AGG - chr8_1:17527028-17527047 None:intergenic 45.0%
CTATAAAACGGCTGAGACAG+TGG - chr8_1:17527042-17527061 None:intergenic 45.0%
TTATTGCACGTCACATGCAG+TGG + chr8_1:17527485-17527504 Msa1182420:five_prime_UTR 45.0%
GGAGAACTAAGCCTAACTAG+AGG + chr8_1:17527506-17527525 Msa1182420:five_prime_UTR 45.0%
TTCCTCAAGTCCCTCTAGTT+AGG - chr8_1:17527520-17527539 None:intergenic 45.0%
GCTTACGAGAGGGTGAATTT+AGG + chr8_1:17527706-17527725 Msa1182420:five_prime_UTR 45.0%
GCTAAAATTAAGCCACCCTC+AGG + chr8_1:17527771-17527790 Msa1182420:five_prime_UTR 45.0%
CACTTCTGTCAATGAGACTG+TGG + chr8_1:17528838-17528857 Msa1182420:three_prime_UTR 45.0%
!! CAGGATACGGACTGGTCTTA+TGG - chr8_1:17526095-17526114 None:intergenic 50.0%
TATGTGTGCAGGATACGGAC+TGG - chr8_1:17526103-17526122 None:intergenic 50.0%
TTCTACGGAAGGGAGGAAAG+TGG + chr8_1:17526901-17526920 Msa1182420:five_prime_UTR 50.0%
AGCCTAACTAGAGGGACTTG+AGG + chr8_1:17527515-17527534 Msa1182420:five_prime_UTR 50.0%
ACAGCTGCACTAAATGCACC+AGG + chr8_1:17528479-17528498 Msa1182420:intron 50.0%
CTGCACTAAATGCACCAGGA+AGG + chr8_1:17528483-17528502 Msa1182420:CDS 50.0%
GCAGAGCAACCTTAGTGCAC+CGG + chr8_1:17526628-17526647 Msa1182420:intron 55.0%
GAGAACCAAGCCTCGCTAGA+GGG + chr8_1:17527623-17527642 Msa1182420:five_prime_UTR 55.0%
GGAGAACCAAGCCTCGCTAG+AGG + chr8_1:17527622-17527641 Msa1182420:five_prime_UTR 60.0%
CAAGTCCCTCTAGCGAGGCT+TGG - chr8_1:17527631-17527650 None:intergenic 60.0%
AGCCTCGCTAGAGGGACTTG+AGG + chr8_1:17527631-17527650 Msa1182420:five_prime_UTR 60.0%
CTCCTCAAGTCCCTCTAGCG+AGG - chr8_1:17527636-17527655 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 17525964 17529089 17525964 ID=Msa1182420;Name=Msa1182420
chr8_1 mRNA 17525964 17529089 17525964 ID=Msa1182420-mRNA-1;Parent=Msa1182420;Name=Msa1182420-mRNA-1;_AED=0.19;_eAED=0.19;_QI=1341|1|1|1|0.66|0.5|4|387|66
chr8_1 exon 17525964 17526017 17525964 ID=Msa1182420-mRNA-1:exon:6541;Parent=Msa1182420-mRNA-1
chr8_1 exon 17526632 17527995 17526632 ID=Msa1182420-mRNA-1:exon:6542;Parent=Msa1182420-mRNA-1
chr8_1 exon 17528483 17528569 17528483 ID=Msa1182420-mRNA-1:exon:6543;Parent=Msa1182420-mRNA-1
chr8_1 exon 17528666 17529089 17528666 ID=Msa1182420-mRNA-1:exon:6544;Parent=Msa1182420-mRNA-1
chr8_1 five_prime_UTR 17525964 17526017 17525964 ID=Msa1182420-mRNA-1:five_prime_utr;Parent=Msa1182420-mRNA-1
chr8_1 five_prime_UTR 17526632 17527918 17526632 ID=Msa1182420-mRNA-1:five_prime_utr;Parent=Msa1182420-mRNA-1
chr8_1 CDS 17527919 17527995 17527919 ID=Msa1182420-mRNA-1:cds;Parent=Msa1182420-mRNA-1
chr8_1 CDS 17528483 17528569 17528483 ID=Msa1182420-mRNA-1:cds;Parent=Msa1182420-mRNA-1
chr8_1 CDS 17528666 17528702 17528666 ID=Msa1182420-mRNA-1:cds;Parent=Msa1182420-mRNA-1
chr8_1 three_prime_UTR 17528703 17529089 17528703 ID=Msa1182420-mRNA-1:three_prime_utr;Parent=Msa1182420-mRNA-1
Gene Sequence

>Msa1182420

ATGTCAAATGCTTGGTCTGGGTCTAAACTGTTTATTAATGATGATTCCATTGAGGAACTCAAACAATTTAAGGAAAGCTGCACTAAATGCACCAGGAAGGCTAAAACTCCAAATGGCCAATTTAAATGTGATTGTGGACAAGTTTTTGAACAGGCAATCCCAAGGTACAAATTTCCAATTGTAGGTCTTTTTATCAAGTAG
Protein sequence

>Msa1182420

MSNAWSGSKLFINDDSIEELKQFKESCTKCTRKAKTPNGQFKCDCGQVFEQAIPRYKFPIVGLFIK*