AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1191210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1191210 MtrunA17_Chr2g0296781 93.913 230 14 0 1 230 348 577 1.05e-156 451
Msa1191210 MtrunA17_Chr6g0477261 91.739 230 19 0 1 230 237 466 1.65e-154 444
Msa1191210 MtrunA17_Chr1g0196541 74.009 227 59 0 1 227 348 574 1.17e-109 331
Msa1191210 MtrunA17_Chr5g0441581 49.302 215 108 1 2 216 377 590 8.90e-66 215
Msa1191210 MtrunA17_Chr6g0476441 89.024 82 9 0 149 230 1 82 5.95e-47 157
Msa1191210 MtrunA17_Chr1g0207551 41.706 211 116 3 1 211 388 591 6.80e-43 154
Msa1191210 MtrunA17_Chr2g0291581 37.441 211 129 3 1 209 412 621 1.32e-41 150
Msa1191210 MtrunA17_Chr5g0448761 38.318 214 126 3 1 214 423 630 3.16e-41 149
Msa1191210 MtrunA17_Chr3g0080351 37.441 211 125 2 1 211 422 625 1.08e-40 147
Msa1191210 MtrunA17_Chr5g0440451 37.500 232 133 5 1 230 404 625 5.40e-40 146
Msa1191210 MtrunA17_Chr3g0118611 37.799 209 122 3 1 209 301 501 1.41e-36 136
Msa1191210 MtrunA17_Chr1g0202341 37.441 211 122 5 1 209 407 609 1.97e-35 132
Msa1191210 MtrunA17_Chr2g0329031 33.178 214 135 3 1 214 361 566 5.49e-35 131
Msa1191210 MtrunA17_Chr5g0448511 33.014 209 134 3 1 209 195 397 1.01e-32 124
Msa1191210 MtrunA17_Chr2g0287061 33.971 209 122 5 5 209 505 701 2.78e-24 100
Msa1191210 MtrunA17_Chr2g0276841 28.696 230 139 8 1 223 432 643 3.11e-19 85.9
Msa1191210 MtrunA17_Chr8g0363841 28.444 225 125 6 1 209 373 577 3.73e-19 85.9
Msa1191210 MtrunA17_Chr2g0287651 27.570 214 146 5 1 212 429 635 2.38e-18 83.6
Msa1191210 MtrunA17_Chr2g0291591 34.959 123 80 0 1 123 415 537 2.50e-18 83.2
Msa1191210 MtrunA17_Chr1g0203051 28.571 203 123 4 1 194 431 620 5.15e-18 82.4
Msa1191210 MtrunA17_Chr1g0198761 29.487 234 149 6 1 229 414 636 6.73e-18 82.0
Msa1191210 MtrunA17_Chr3g0082121 31.068 206 130 6 5 209 396 590 7.74e-17 79.0
Msa1191210 MtrunA17_Chr2g0308841 28.302 212 135 5 1 206 24 224 2.80e-15 72.8
Msa1191210 MtrunA17_Chr2g0324011 24.299 214 146 4 1 209 366 568 3.27e-15 74.3
Msa1191210 MtrunA17_Chr6g0458391 26.540 211 144 4 1 209 564 765 1.55e-14 72.4
Msa1191210 MtrunA17_Chr1g0172041 34.375 96 62 1 121 216 1 95 1.13e-13 65.5
Msa1191210 MtrunA17_Chr2g0290391 26.432 227 144 7 1 225 421 626 1.74e-13 69.3
Msa1191210 MtrunA17_Chr1g0203721 25.359 209 149 3 1 209 422 623 1.93e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1191210 AT4G15090.1 38.288 222 129 3 1 222 408 621 5.02e-43 155
Msa1191210 AT4G15090.5 38.288 222 129 3 1 222 408 621 5.02e-43 155
Msa1191210 AT4G15090.3 38.288 222 129 3 1 222 408 621 5.02e-43 155
Msa1191210 AT4G15090.2 38.288 222 129 3 1 222 408 621 5.02e-43 155
Msa1191210 AT4G15090.4 38.288 222 129 3 1 222 408 621 5.02e-43 155
Msa1191210 AT3G22170.2 39.423 208 119 3 5 212 421 621 9.70e-43 154
Msa1191210 AT3G22170.1 39.423 208 119 3 5 212 421 621 9.70e-43 154
Msa1191210 AT4G38180.1 36.364 209 127 4 1 209 432 634 6.27e-39 143
Msa1191210 AT1G52520.1 36.574 216 125 7 1 209 428 638 6.46e-38 140
Msa1191210 AT4G19990.1 35.349 215 132 3 1 214 299 507 1.37e-36 136
Msa1191210 AT4G19990.3 35.349 215 132 3 1 214 356 564 1.44e-36 136
Msa1191210 AT4G19990.4 35.349 215 132 3 1 214 356 564 1.44e-36 136
Msa1191210 AT4G19990.5 35.349 215 132 3 1 214 356 564 1.63e-36 136
Msa1191210 AT4G19990.7 35.349 215 132 3 1 214 370 578 1.74e-36 136
Msa1191210 AT4G19990.6 35.349 215 132 3 1 214 356 564 1.94e-36 136
Msa1191210 AT4G19990.2 35.349 215 132 3 1 214 356 564 1.94e-36 136
Msa1191210 AT1G76320.2 34.259 216 133 4 1 216 345 551 1.20e-34 130
Msa1191210 AT1G76320.1 34.259 216 133 4 1 216 345 551 1.25e-34 130
Msa1191210 AT1G76320.4 34.259 216 133 4 1 216 345 551 1.25e-34 130
Msa1191210 AT1G76320.3 34.259 216 133 4 1 216 345 551 1.35e-34 130
Msa1191210 AT4G38170.1 31.455 213 140 2 1 213 197 403 1.83e-32 124
Msa1191210 AT2G32250.5 33.981 206 129 2 1 206 352 550 3.79e-30 118
Msa1191210 AT2G32250.8 33.981 206 129 2 1 206 311 509 3.83e-30 118
Msa1191210 AT2G32250.7 33.981 206 129 2 1 206 311 509 4.07e-30 117
Msa1191210 AT2G32250.2 33.981 206 129 2 1 206 352 550 4.22e-30 117
Msa1191210 AT2G32250.4 33.981 206 129 2 1 206 352 550 4.22e-30 117
Msa1191210 AT2G32250.6 33.981 206 129 2 1 206 352 550 4.31e-30 117
Msa1191210 AT2G32250.1 33.981 206 129 2 1 206 352 550 4.31e-30 117
Msa1191210 AT2G32250.3 33.981 206 129 2 1 206 352 550 4.31e-30 117
Msa1191210 AT2G27110.3 35.407 209 127 6 1 209 234 434 3.70e-29 115
Msa1191210 AT1G80010.1 32.618 233 148 6 1 229 453 680 5.46e-29 114
Msa1191210 AT2G27110.2 35.407 209 127 6 1 209 379 579 5.67e-29 114
Msa1191210 AT2G27110.1 35.407 209 127 6 1 209 379 579 5.67e-29 114
Msa1191210 AT3G06250.2 31.100 209 135 3 1 209 505 704 1.31e-24 102
Msa1191210 AT3G06250.1 31.100 209 135 3 1 209 505 704 1.31e-24 102
Msa1191210 AT3G06250.3 31.100 209 135 3 1 209 505 704 1.31e-24 102
Msa1191210 AT5G18960.2 32.381 210 131 5 1 209 529 728 1.86e-24 101
Msa1191210 AT5G18960.1 32.381 210 131 5 1 209 529 728 1.86e-24 101
Msa1191210 AT1G10240.2 30.516 213 123 8 1 206 410 604 4.02e-20 89.0
Msa1191210 AT1G10240.1 30.516 213 123 8 1 206 410 604 4.02e-20 89.0

Find 35 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCTTGTTTGAATTTAA+AGG 0.112904 8_1:-35218595 Msa1191210:CDS
CATATGTAAGGGACACATTT+TGG 0.131035 8_1:-35219023 Msa1191210:CDS
ATTTACCAATGCAAAGTTTA+AGG 0.134414 8_1:-35218788 Msa1191210:CDS
TGTGCCATCTAATTATATTT+TGG 0.166070 8_1:-35218518 Msa1191210:CDS
TAAATGTGGTGTTGATAATT+TGG 0.259658 8_1:-35218455 Msa1191210:CDS
TTTCAACAGATTGGAGGATT+TGG 0.276962 8_1:-35218722 Msa1191210:CDS
CTTTGTGTGCTTAAGCTCAT+AGG 0.292164 8_1:-35218553 Msa1191210:CDS
GATGAAAGATATTGTGTTCA+TGG 0.400462 8_1:-35218656 Msa1191210:CDS
ATTTCTTGTATTAGTCACTT+TGG 0.417540 8_1:-35218835 Msa1191210:CDS
TCATGCATGTTTCAACAGAT+TGG 0.428105 8_1:-35218731 Msa1191210:CDS
ATTTGTTGAGCGACATCGTT+GGG 0.428935 8_1:-35219052 Msa1191210:CDS
CGTGCCAAAATATAATTAGA+TGG 0.452638 8_1:+35218514 None:intergenic
GTTGGGTCCCTGCATATGTA+AGG 0.456247 8_1:-35219035 Msa1191210:CDS
AAAGTTTAAGGAATTCCAAT+TGG 0.460527 8_1:-35218776 Msa1191210:CDS
TCATAGAAGCTATTTCCAAT+TGG 0.475525 8_1:+35218761 None:intergenic
AATGTGTCCCTTACATATGC+AGG 0.477892 8_1:+35219027 None:intergenic
TTTGTGTGCTTAAGCTCATA+GGG 0.489887 8_1:-35218552 Msa1191210:CDS
TTTAAATTCAAACAAGCAGC+AGG 0.496973 8_1:+35218597 None:intergenic
AGGATAACGAATGGCTGAAA+GGG 0.500694 8_1:-35219077 Msa1191210:CDS
ATTGAAAGATAAAATTGAAA+AGG 0.501824 8_1:-35218893 Msa1191210:CDS
GAATTCCTTAAACTTTGCAT+TGG 0.503666 8_1:+35218783 None:intergenic
TGCATGTTTCAACAGATTGG+AGG 0.514840 8_1:-35218728 Msa1191210:CDS
CAGGATAACGAATGGCTGAA+AGG 0.539689 8_1:-35219078 Msa1191210:CDS
GATTGAGTGTTACAAACTAC+AGG 0.556519 8_1:-35219097 Msa1191210:CDS
TATCAACACCACATTTAACA+AGG 0.590139 8_1:+35218461 None:intergenic
TATTTGTTGAGCGACATCGT+TGG 0.591607 8_1:-35219053 Msa1191210:CDS
TTGGGTCCCTGCATATGTAA+GGG 0.619298 8_1:-35219034 Msa1191210:CDS
ATGTGTCCCTTACATATGCA+GGG 0.621051 8_1:+35219028 None:intergenic
AGGTATACCCTTGTTAAATG+TGG 0.622387 8_1:-35218469 Msa1191210:CDS
ATCAACACCACATTTAACAA+GGG 0.638905 8_1:+35218462 None:intergenic
ACAAACTACAGGATAACGAA+TGG 0.644106 8_1:-35219086 Msa1191210:CDS
CATTTAACAAGGGTATACCT+CGG 0.672880 8_1:+35218472 None:intergenic
CTATGAGCTTAAGCACACAA+AGG 0.703144 8_1:+35218554 None:intergenic
GGAGGAAGGATATAAAGCCG+AGG 0.711941 8_1:-35218489 Msa1191210:CDS
TTTATGAATTTGCTTCAACG+AGG 0.719121 8_1:-35218430 Msa1191210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAAGATAAAATTGAAA+AGG - chr8_1:35218632-35218651 Msa1191210:CDS 15.0%
!!! AATTTAAAGGCATTTTATGT+AGG - chr8_1:35218943-35218962 Msa1191210:CDS 20.0%
!!! AGTATGAACTCATTTTTTGA+TGG - chr8_1:35218555-35218574 Msa1191210:CDS 25.0%
! ATTTCTTGTATTAGTCACTT+TGG - chr8_1:35218690-35218709 Msa1191210:CDS 25.0%
! ATTTACCAATGCAAAGTTTA+AGG - chr8_1:35218737-35218756 Msa1191210:CDS 25.0%
! AAAGTTTAAGGAATTCCAAT+TGG - chr8_1:35218749-35218768 Msa1191210:CDS 25.0%
!! TGTGCCATCTAATTATATTT+TGG - chr8_1:35219007-35219026 Msa1191210:CDS 25.0%
!!! CATCTAATTATATTTTGGCA+CGG - chr8_1:35219012-35219031 Msa1191210:CDS 25.0%
!!! TAAATGTGGTGTTGATAATT+TGG - chr8_1:35219070-35219089 Msa1191210:CDS 25.0%
! ATATGTAAGGGACACATTTT+GGG - chr8_1:35218503-35218522 Msa1191210:CDS 30.0%
!!! TGGTAAATGCTTTTTGGAAT+TGG + chr8_1:35218725-35218744 None:intergenic 30.0%
!! TTGCATTGGTAAATGCTTTT+TGG + chr8_1:35218731-35218750 None:intergenic 30.0%
GAATTCCTTAAACTTTGCAT+TGG + chr8_1:35218745-35218764 None:intergenic 30.0%
! TCATAGAAGCTATTTCCAAT+TGG + chr8_1:35218767-35218786 None:intergenic 30.0%
GATGAAAGATATTGTGTTCA+TGG - chr8_1:35218869-35218888 Msa1191210:CDS 30.0%
TTTAAATTCAAACAAGCAGC+AGG + chr8_1:35218931-35218950 None:intergenic 30.0%
! TGCTGCTTGTTTGAATTTAA+AGG - chr8_1:35218930-35218949 Msa1191210:CDS 30.0%
CGTGCCAAAATATAATTAGA+TGG + chr8_1:35219014-35219033 None:intergenic 30.0%
ATCAACACCACATTTAACAA+GGG + chr8_1:35219066-35219085 None:intergenic 30.0%
TATCAACACCACATTTAACA+AGG + chr8_1:35219067-35219086 None:intergenic 30.0%
! TTTATGAATTTGCTTCAACG+AGG - chr8_1:35219095-35219114 Msa1191210:CDS 30.0%
GATTGAGTGTTACAAACTAC+AGG - chr8_1:35218428-35218447 Msa1191210:CDS 35.0%
ACAAACTACAGGATAACGAA+TGG - chr8_1:35218439-35218458 Msa1191210:CDS 35.0%
! CATATGTAAGGGACACATTT+TGG - chr8_1:35218502-35218521 Msa1191210:CDS 35.0%
TCATGCATGTTTCAACAGAT+TGG - chr8_1:35218794-35218813 Msa1191210:CDS 35.0%
TTTCAACAGATTGGAGGATT+TGG - chr8_1:35218803-35218822 Msa1191210:CDS 35.0%
TTTGTGTGCTTAAGCTCATA+GGG - chr8_1:35218973-35218992 Msa1191210:CDS 35.0%
!!! CTAATTATATTTTGGCACGG+TGG - chr8_1:35219015-35219034 Msa1191210:CDS 35.0%
! CATTTAACAAGGGTATACCT+CGG + chr8_1:35219056-35219075 None:intergenic 35.0%
AGGTATACCCTTGTTAAATG+TGG - chr8_1:35219056-35219075 Msa1191210:CDS 35.0%
AGGATAACGAATGGCTGAAA+GGG - chr8_1:35218448-35218467 Msa1191210:CDS 40.0%
! TATTTGTTGAGCGACATCGT+TGG - chr8_1:35218472-35218491 Msa1191210:CDS 40.0%
! ATTTGTTGAGCGACATCGTT+GGG - chr8_1:35218473-35218492 Msa1191210:CDS 40.0%
ATGTGTCCCTTACATATGCA+GGG + chr8_1:35218500-35218519 None:intergenic 40.0%
AATGTGTCCCTTACATATGC+AGG + chr8_1:35218501-35218520 None:intergenic 40.0%
TGCATGTTTCAACAGATTGG+AGG - chr8_1:35218797-35218816 Msa1191210:CDS 40.0%
CTATGAGCTTAAGCACACAA+AGG + chr8_1:35218974-35218993 None:intergenic 40.0%
CTTTGTGTGCTTAAGCTCAT+AGG - chr8_1:35218972-35218991 Msa1191210:CDS 40.0%
!!! ATTATATTTTGGCACGGTGG+AGG - chr8_1:35219018-35219037 Msa1191210:CDS 40.0%
CAGGATAACGAATGGCTGAA+AGG - chr8_1:35218447-35218466 Msa1191210:CDS 45.0%
TTGGGTCCCTGCATATGTAA+GGG - chr8_1:35218491-35218510 Msa1191210:CDS 45.0%
GTTGGGTCCCTGCATATGTA+AGG - chr8_1:35218490-35218509 Msa1191210:CDS 50.0%
! GTAAGGGACACATTTTGGGC+TGG - chr8_1:35218507-35218526 Msa1191210:CDS 50.0%
!!! TATTTTGGCACGGTGGAGGA+AGG - chr8_1:35219022-35219041 Msa1191210:CDS 50.0%
GGAGGAAGGATATAAAGCCG+AGG - chr8_1:35219036-35219055 Msa1191210:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 35218426 35219121 35218426 ID=Msa1191210;Name=Msa1191210
chr8_1 mRNA 35218426 35219121 35218426 ID=Msa1191210-mRNA-1;Parent=Msa1191210;Name=Msa1191210-mRNA-1;_AED=0.95;_eAED=0.95;_QI=0|-1|0|1|-1|1|1|0|231
chr8_1 exon 35218426 35219121 35218426 ID=Msa1191210-mRNA-1:exon:11677;Parent=Msa1191210-mRNA-1
chr8_1 CDS 35218426 35219121 35218426 ID=Msa1191210-mRNA-1:cds;Parent=Msa1191210-mRNA-1
Gene Sequence

>Msa1191210

ATGATTGAGTGTTACAAACTACAGGATAACGAATGGCTGAAAGGGTTATTTGTTGAGCGACATCGTTGGGTCCCTGCATATGTAAGGGACACATTTTGGGCTGGAATGTCAACTACACAACGAAGTGAAAGTATGAACTCATTTTTTGATGGATATGTAAGCTCAAAGACAACATTGAAGCAATTTGTTGAGCAATATGATAATGCATTGAAAGATAAAATTGAAAAGGAAAACATTGCTGACTTTCGTTCTTTTAATACAATCATTTCTTGTATTAGTCACTTTGGATTTGAGTTCCAATTCCAAAAAGCATTTACCAATGCAAAGTTTAAGGAATTCCAATTGGAAATAGCTTCTATGATGTACTGTCATGCATGTTTCAACAGATTGGAGGATTTGGATTCAATATTTTCTGTTACAGAAAGTAAGAAAGTATATGACAAGATGAAAGATATTGTGTTCATGGTGTTCTTCAATGAAAAAGACTTTGTGCTGAAATGCACCTGCTGCTTGTTTGAATTTAAAGGCATTTTATGTAGGCACATCCTTTGTGTGCTTAAGCTCATAGGGAAAATAGATTTTGTGCCATCTAATTATATTTTGGCACGGTGGAGGAAGGATATAAAGCCGAGGTATACCCTTGTTAAATGTGGTGTTGATAATTTGGCTTTTATGAATTTGCTTCAACGAGGATAA

Protein sequence

>Msa1191210

MIECYKLQDNEWLKGLFVERHRWVPAYVRDTFWAGMSTTQRSESMNSFFDGYVSSKTTLKQFVEQYDNALKDKIEKENIADFRSFNTIISCISHFGFEFQFQKAFTNAKFKEFQLEIASMMYCHACFNRLEDLDSIFSVTESKKVYDKMKDIVFMVFFNEKDFVLKCTCCLFEFKGILCRHILCVLKLIGKIDFVPSNYILARWRKDIKPRYTLVKCGVDNLAFMNLLQRG*