AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1201260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201260 MtrunA17_Chr8g0371131 90.141 142 14 0 1 142 1 142 4.49e-93 265
Msa1201260 MtrunA17_Chr8g0346021 62.295 61 23 0 69 129 125 185 4.34e-18 79.3
Msa1201260 MtrunA17_Chr1g0204901 56.923 65 28 0 70 134 135 199 2.29e-17 77.4
Msa1201260 MtrunA17_Chr8g0389781 56.716 67 29 0 70 136 125 191 2.62e-17 77.0
Msa1201260 MtrunA17_Chr1g0203711 62.903 62 23 0 70 131 114 175 5.88e-17 76.3
Msa1201260 MtrunA17_Chr2g0330921 50.649 77 38 0 63 139 89 165 6.02e-17 75.9
Msa1201260 MtrunA17_Chr8g0340761 50.704 71 35 0 62 132 117 187 1.57e-16 74.7
Msa1201260 MtrunA17_Chr1g0200161 53.846 65 30 0 70 134 140 204 2.21e-16 74.7
Msa1201260 MtrunA17_Chr1g0146931 63.636 55 20 0 68 122 119 173 6.62e-16 73.2
Msa1201260 MtrunA17_Chr4g0020671 47.945 73 35 1 71 140 101 173 1.75e-15 72.0
Msa1201260 MtrunA17_Chr6g0472551 61.290 62 24 0 73 134 119 180 2.05e-15 71.6
Msa1201260 MtrunA17_Chr3g0126901 63.636 55 20 0 68 122 109 163 2.47e-15 71.6
Msa1201260 MtrunA17_Chr7g0273951 58.065 62 26 0 73 134 108 169 3.72e-15 70.9
Msa1201260 MtrunA17_Chr4g0060381 56.667 60 26 0 73 132 102 161 4.82e-15 70.9
Msa1201260 MtrunA17_Chr4g0025441 66.038 53 18 0 70 122 95 147 1.30e-14 69.3
Msa1201260 MtrunA17_Chr7g0273861 49.231 65 33 0 70 134 124 188 2.68e-14 68.6
Msa1201260 MtrunA17_Chr1g0156121 34.921 126 72 2 18 133 61 186 1.01e-13 67.0
Msa1201260 MtrunA17_Chr4g0057321 47.945 73 38 0 59 131 76 148 3.60e-13 65.5
Msa1201260 MtrunA17_Chr4g0045641 44.118 68 38 0 67 134 142 209 7.57e-13 64.3
Msa1201260 MtrunA17_Chr3g0135851 38.211 123 66 3 2 122 28 142 2.48e-12 62.8
Msa1201260 MtrunA17_Chr6g0462191 52.778 72 34 0 69 140 105 176 9.03e-12 61.2
Msa1201260 MtrunA17_Chr1g0166431 55.556 54 24 0 70 123 116 169 2.15e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201260 AT3G51380.1 50.735 136 33 2 1 136 1 102 5.29e-34 115
Msa1201260 AT3G49260.1 60.563 71 28 0 69 139 118 188 8.51e-21 87.4
Msa1201260 AT3G49260.4 60.563 71 28 0 69 139 118 188 8.51e-21 87.4
Msa1201260 AT3G49260.2 60.563 71 28 0 69 139 118 188 8.51e-21 87.4
Msa1201260 AT3G49260.3 60.563 71 28 0 69 139 118 188 8.53e-21 87.4
Msa1201260 AT1G01110.2 55.128 78 35 0 65 142 115 192 1.15e-19 84.3
Msa1201260 AT4G00820.1 58.108 74 31 0 69 142 130 203 3.20e-19 83.2
Msa1201260 AT4G14750.1 61.538 65 25 0 70 134 104 168 1.01e-18 81.3
Msa1201260 AT4G14750.3 61.538 65 25 0 70 134 163 227 1.21e-18 81.3
Msa1201260 AT4G14750.2 61.538 65 25 0 70 134 163 227 1.29e-18 81.3
Msa1201260 AT3G52290.1 54.286 70 32 0 70 139 107 176 1.14e-17 78.6
Msa1201260 AT3G59690.2 57.377 61 26 0 74 134 172 232 6.90e-16 73.6
Msa1201260 AT3G59690.1 57.377 61 26 0 74 134 172 232 6.90e-16 73.6
Msa1201260 AT1G51960.1 47.945 73 38 0 62 134 91 163 1.77e-15 72.0
Msa1201260 AT1G72670.1 53.968 63 29 0 69 131 91 153 1.81e-15 72.4
Msa1201260 AT5G07240.1 58.462 65 27 0 67 131 102 166 1.98e-15 72.0
Msa1201260 AT3G16490.1 53.846 65 30 0 70 134 106 170 3.32e-15 71.6
Msa1201260 AT1G17480.1 44.186 86 43 1 46 131 74 154 3.71e-15 71.2
Msa1201260 AT5G07240.2 58.462 65 27 0 67 131 148 212 3.82e-15 71.2
Msa1201260 AT4G10640.1 57.143 63 27 0 67 129 96 158 4.25e-15 71.2
Msa1201260 AT2G26180.1 52.113 71 29 1 52 122 70 135 5.00e-15 70.9
Msa1201260 AT1G18840.1 40.909 88 50 1 47 134 87 172 5.11e-15 71.2
Msa1201260 AT1G18840.3 40.909 88 50 1 47 134 87 172 5.11e-15 71.2
Msa1201260 AT1G18840.2 40.909 88 50 1 47 134 87 172 5.11e-15 71.2
Msa1201260 AT4G23060.1 53.846 65 30 0 71 135 167 231 1.75e-14 69.7
Msa1201260 AT5G13460.1 52.459 61 29 0 69 129 109 169 1.91e-14 69.3
Msa1201260 AT5G62070.1 61.017 59 23 0 67 125 113 171 2.42e-14 68.9
Msa1201260 AT5G03040.1 47.761 67 35 0 68 134 112 178 6.82e-14 67.8
Msa1201260 AT5G03040.3 47.761 67 35 0 68 134 112 178 6.82e-14 67.8
Msa1201260 AT5G03040.2 47.761 67 35 0 68 134 112 178 6.82e-14 67.8
Msa1201260 AT2G43680.1 48.387 62 32 0 73 134 325 386 8.97e-14 67.4
Msa1201260 AT2G43680.4 48.387 62 32 0 73 134 324 385 9.06e-14 67.4
Msa1201260 AT2G43680.2 48.387 62 32 0 73 134 324 385 9.06e-14 67.4
Msa1201260 AT2G43680.3 48.387 62 32 0 73 134 324 385 9.06e-14 67.4
Msa1201260 AT2G43680.5 48.387 62 32 0 73 134 324 385 9.06e-14 67.4
Msa1201260 AT3G22190.3 58.182 55 23 0 68 122 85 139 1.69e-13 66.6
Msa1201260 AT3G22190.2 58.182 55 23 0 68 122 85 139 1.69e-13 66.6
Msa1201260 AT3G22190.4 58.182 55 23 0 68 122 85 139 1.69e-13 66.6
Msa1201260 AT3G22190.1 58.182 55 23 0 68 122 85 139 1.69e-13 66.6
Msa1201260 AT1G14380.1 49.180 61 31 0 73 133 96 156 5.72e-13 65.1
Msa1201260 AT1G14380.3 49.180 61 31 0 73 133 96 156 5.72e-13 65.1
Msa1201260 AT1G14380.4 49.180 61 31 0 73 133 96 156 5.72e-13 65.1
Msa1201260 AT3G09710.1 37.113 97 53 2 52 140 81 177 6.92e-13 65.1
Msa1201260 AT3G09710.2 37.113 97 53 2 52 140 81 177 7.39e-13 64.7
Msa1201260 AT4G29150.1 50.000 68 34 0 68 135 128 195 8.80e-13 64.7
Msa1201260 AT1G14380.6 49.180 61 31 0 73 133 96 156 1.06e-12 64.3
Msa1201260 AT1G14380.5 49.180 61 31 0 73 133 96 156 1.06e-12 64.3
Msa1201260 AT1G14380.2 49.180 61 31 0 73 133 96 156 1.06e-12 64.3
Msa1201260 AT1G14380.7 49.180 61 31 0 73 133 96 156 1.57e-12 63.9
Msa1201260 AT1G74690.1 46.377 69 37 0 69 137 111 179 2.84e-12 63.2
Msa1201260 AT3G49380.1 58.065 62 26 0 66 127 105 166 3.55e-12 62.8

Find 32 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAGATTCAAGCTTATTTC+AGG 0.205987 8_1:+54446620 Msa1201260:CDS
AACGATTCCCAACCATTTCT+TGG 0.217793 8_1:-54446407 None:intergenic
CAAAGCCAACAAATCATATT+TGG 0.218301 8_1:+54446499 Msa1201260:CDS
AAATGGTTATACTTACTAAT+TGG 0.258617 8_1:-54446976 None:intergenic
CATTTCCAAATATGATTTGT+TGG 0.353157 8_1:-54446504 None:intergenic
CATTTATATGAATATACGTT+TGG 0.367352 8_1:+54447157 Msa1201260:three_prime_UTR
CCTTGTGAAATTGCAAGCAT+TGG 0.437449 8_1:+54446821 Msa1201260:CDS
AATTGCAAGCATTGGTTCGC+GGG 0.441109 8_1:+54446829 Msa1201260:CDS
AAATGGGTGTATCCAAGAAA+TGG 0.457505 8_1:+54446395 None:intergenic
CAAGCTGAGTAAATCTTCTC+TGG 0.466247 8_1:-54446539 None:intergenic
TAAGATTCAAGCTTATTTCA+GGG 0.474748 8_1:+54446621 Msa1201260:CDS
GGTGTATCCAAGAAATGGTT+GGG 0.495546 8_1:+54446400 Msa1201260:CDS
GCAGGCAAGAAGAGCACATA+GGG 0.496706 8_1:+54446788 Msa1201260:intron
AAATTGCAAGCATTGGTTCG+CGG 0.525909 8_1:+54446828 Msa1201260:CDS
GAGTAAATCTTCTCTGGTGG+TGG 0.527033 8_1:-54446533 None:intergenic
CCAATGCTTGCAATTTCACA+AGG 0.529073 8_1:-54446821 None:intergenic
GAAAATATATGTCAACTAGT+TGG 0.544271 8_1:-54447247 None:intergenic
TGCAAACACTTATACATGGA+TGG 0.548009 8_1:-54446471 None:intergenic
TGGTTCGCGGGGTGTGTGTG+AGG 0.555126 8_1:+54446841 Msa1201260:CDS
TTTGTGCAAACACTTATACA+TGG 0.556057 8_1:-54446475 None:intergenic
GGGTGTATCCAAGAAATGGT+TGG 0.579174 8_1:+54446399 Msa1201260:CDS
AAGATTCAAGCTTATTTCAG+GGG 0.590774 8_1:+54446622 Msa1201260:CDS
ACTTGTTCGATTACAAGTCA+AGG 0.596527 8_1:+54446899 Msa1201260:CDS
TACAAGTCAAGGTTCGTGCA+AGG 0.617179 8_1:+54446910 Msa1201260:CDS
GAAGCATTAATATGTGGTGG+TGG 0.625883 8_1:-54446562 None:intergenic
GTTCGTGCAAGGCAATTACT+CGG 0.631455 8_1:+54446921 Msa1201260:CDS
ATTGCAAGCATTGGTTCGCG+GGG 0.636033 8_1:+54446830 Msa1201260:CDS
CAATGTGTACATATATTGTG+AGG 0.636610 8_1:+54447114 Msa1201260:three_prime_UTR
TTATATAATATTGAAAACTG+AGG 0.638029 8_1:+54447013 Msa1201260:three_prime_UTR
ATCAATGAAGCATTAATATG+TGG 0.643274 8_1:-54446568 None:intergenic
AGCAGGCAAGAAGAGCACAT+AGG 0.644791 8_1:+54446787 Msa1201260:intron
AATGAAGCATTAATATGTGG+TGG 0.681570 8_1:-54446565 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTCAATATTATATAAAT+CGG - chr8_1:54447011-54447030 None:intergenic 10.0%
!!! GTTTTCAATATTATATAAAT+CGG - chr8_1:54447011-54447030 None:intergenic 10.0%
!! TTATATAATATTGAAAACTG+AGG + chr8_1:54447013-54447032 Msa1201260:three_prime_UTR 15.0%
!! TTATATAATATTGAAAACTG+AGG + chr8_1:54447013-54447032 Msa1201260:three_prime_UTR 15.0%
!! AAATGGTTATACTTACTAAT+TGG - chr8_1:54446979-54446998 None:intergenic 20.0%
!! CATTTATATGAATATACGTT+TGG + chr8_1:54447157-54447176 Msa1201260:three_prime_UTR 20.0%
!! AAATGGTTATACTTACTAAT+TGG - chr8_1:54446979-54446998 None:intergenic 20.0%
!! CATTTATATGAATATACGTT+TGG + chr8_1:54447157-54447176 Msa1201260:three_prime_UTR 20.0%
!! CATTTCCAAATATGATTTGT+TGG - chr8_1:54446507-54446526 None:intergenic 25.0%
!! ATCAATGAAGCATTAATATG+TGG - chr8_1:54446571-54446590 None:intergenic 25.0%
! TTAAGATTCAAGCTTATTTC+AGG + chr8_1:54446620-54446639 Msa1201260:CDS 25.0%
! TAAGATTCAAGCTTATTTCA+GGG + chr8_1:54446621-54446640 Msa1201260:CDS 25.0%
! TAAATCGGATATGAGAAAAA+TGG - chr8_1:54446996-54447015 None:intergenic 25.0%
!! CATTTCCAAATATGATTTGT+TGG - chr8_1:54446507-54446526 None:intergenic 25.0%
!! ATCAATGAAGCATTAATATG+TGG - chr8_1:54446571-54446590 None:intergenic 25.0%
! TTAAGATTCAAGCTTATTTC+AGG + chr8_1:54446620-54446639 Msa1201260:CDS 25.0%
! TAAGATTCAAGCTTATTTCA+GGG + chr8_1:54446621-54446640 Msa1201260:CDS 25.0%
! TAAATCGGATATGAGAAAAA+TGG - chr8_1:54446996-54447015 None:intergenic 25.0%
!!! TGTCTTTTTTGGATGATCTT+AGG - chr8_1:54446444-54446463 None:intergenic 30.0%
!!! ATGGAGAGTTATGTCTTTTT+TGG - chr8_1:54446455-54446474 None:intergenic 30.0%
TTTGTGCAAACACTTATACA+TGG - chr8_1:54446478-54446497 None:intergenic 30.0%
CAAAGCCAACAAATCATATT+TGG + chr8_1:54446499-54446518 Msa1201260:CDS 30.0%
! AATGAAGCATTAATATGTGG+TGG - chr8_1:54446568-54446587 None:intergenic 30.0%
AAGATTCAAGCTTATTTCAG+GGG + chr8_1:54446622-54446641 Msa1201260:CDS 30.0%
ACTATTGAATTGAATTGAGC+AGG + chr8_1:54446770-54446789 Msa1201260:intron 30.0%
CAATGTGTACATATATTGTG+AGG + chr8_1:54447114-54447133 Msa1201260:three_prime_UTR 30.0%
!!! TGTCTTTTTTGGATGATCTT+AGG - chr8_1:54446444-54446463 None:intergenic 30.0%
!!! ATGGAGAGTTATGTCTTTTT+TGG - chr8_1:54446455-54446474 None:intergenic 30.0%
TTTGTGCAAACACTTATACA+TGG - chr8_1:54446478-54446497 None:intergenic 30.0%
CAAAGCCAACAAATCATATT+TGG + chr8_1:54446499-54446518 Msa1201260:CDS 30.0%
! AATGAAGCATTAATATGTGG+TGG - chr8_1:54446568-54446587 None:intergenic 30.0%
AAGATTCAAGCTTATTTCAG+GGG + chr8_1:54446622-54446641 Msa1201260:CDS 30.0%
ACTATTGAATTGAATTGAGC+AGG + chr8_1:54446770-54446789 Msa1201260:intron 30.0%
CAATGTGTACATATATTGTG+AGG + chr8_1:54447114-54447133 Msa1201260:three_prime_UTR 30.0%
TGCAAACACTTATACATGGA+TGG - chr8_1:54446474-54446493 None:intergenic 35.0%
ACTTGTTCGATTACAAGTCA+AGG + chr8_1:54446899-54446918 Msa1201260:CDS 35.0%
TGCAAACACTTATACATGGA+TGG - chr8_1:54446474-54446493 None:intergenic 35.0%
ACTTGTTCGATTACAAGTCA+AGG + chr8_1:54446899-54446918 Msa1201260:CDS 35.0%
GGTGTATCCAAGAAATGGTT+GGG + chr8_1:54446400-54446419 Msa1201260:CDS 40.0%
AACGATTCCCAACCATTTCT+TGG - chr8_1:54446410-54446429 None:intergenic 40.0%
CAAGCTGAGTAAATCTTCTC+TGG - chr8_1:54446542-54446561 None:intergenic 40.0%
! GAAGCATTAATATGTGGTGG+TGG - chr8_1:54446565-54446584 None:intergenic 40.0%
CCAATGCTTGCAATTTCACA+AGG - chr8_1:54446824-54446843 None:intergenic 40.0%
CCTTGTGAAATTGCAAGCAT+TGG + chr8_1:54446821-54446840 Msa1201260:CDS 40.0%
!! AAATTGCAAGCATTGGTTCG+CGG + chr8_1:54446828-54446847 Msa1201260:CDS 40.0%
GGTGTATCCAAGAAATGGTT+GGG + chr8_1:54446400-54446419 Msa1201260:CDS 40.0%
AACGATTCCCAACCATTTCT+TGG - chr8_1:54446410-54446429 None:intergenic 40.0%
CAAGCTGAGTAAATCTTCTC+TGG - chr8_1:54446542-54446561 None:intergenic 40.0%
! GAAGCATTAATATGTGGTGG+TGG - chr8_1:54446565-54446584 None:intergenic 40.0%
CCAATGCTTGCAATTTCACA+AGG - chr8_1:54446824-54446843 None:intergenic 40.0%
CCTTGTGAAATTGCAAGCAT+TGG + chr8_1:54446821-54446840 Msa1201260:CDS 40.0%
!! AAATTGCAAGCATTGGTTCG+CGG + chr8_1:54446828-54446847 Msa1201260:CDS 40.0%
GGGTGTATCCAAGAAATGGT+TGG + chr8_1:54446399-54446418 Msa1201260:CDS 45.0%
GAGTAAATCTTCTCTGGTGG+TGG - chr8_1:54446536-54446555 None:intergenic 45.0%
GCTGAGTAAATCTTCTCTGG+TGG - chr8_1:54446539-54446558 None:intergenic 45.0%
!! AATTGCAAGCATTGGTTCGC+GGG + chr8_1:54446829-54446848 Msa1201260:CDS 45.0%
TACAAGTCAAGGTTCGTGCA+AGG + chr8_1:54446910-54446929 Msa1201260:CDS 45.0%
GTTCGTGCAAGGCAATTACT+CGG + chr8_1:54446921-54446940 Msa1201260:CDS 45.0%
GGGTGTATCCAAGAAATGGT+TGG + chr8_1:54446399-54446418 Msa1201260:CDS 45.0%
GAGTAAATCTTCTCTGGTGG+TGG - chr8_1:54446536-54446555 None:intergenic 45.0%
GCTGAGTAAATCTTCTCTGG+TGG - chr8_1:54446539-54446558 None:intergenic 45.0%
!! AATTGCAAGCATTGGTTCGC+GGG + chr8_1:54446829-54446848 Msa1201260:CDS 45.0%
TACAAGTCAAGGTTCGTGCA+AGG + chr8_1:54446910-54446929 Msa1201260:CDS 45.0%
GTTCGTGCAAGGCAATTACT+CGG + chr8_1:54446921-54446940 Msa1201260:CDS 45.0%
! AGCAGGCAAGAAGAGCACAT+AGG + chr8_1:54446787-54446806 Msa1201260:intron 50.0%
! GCAGGCAAGAAGAGCACATA+GGG + chr8_1:54446788-54446807 Msa1201260:intron 50.0%
!! ATTGCAAGCATTGGTTCGCG+GGG + chr8_1:54446830-54446849 Msa1201260:CDS 50.0%
! AGCAGGCAAGAAGAGCACAT+AGG + chr8_1:54446787-54446806 Msa1201260:intron 50.0%
! GCAGGCAAGAAGAGCACATA+GGG + chr8_1:54446788-54446807 Msa1201260:intron 50.0%
!! ATTGCAAGCATTGGTTCGCG+GGG + chr8_1:54446830-54446849 Msa1201260:CDS 50.0%
TGGTTCGCGGGGTGTGTGTG+AGG + chr8_1:54446841-54446860 Msa1201260:CDS 65.0%
TGGTTCGCGGGGTGTGTGTG+AGG + chr8_1:54446841-54446860 Msa1201260:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 54446397 54447264 54446397 ID=Msa1201260;Name=Msa1201260
chr8_1 mRNA 54446397 54447264 54446397 ID=Msa1201260-mRNA-1;Parent=Msa1201260;Name=Msa1201260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|299|142
chr8_1 exon 54446397 54446651 54446397 ID=Msa1201260-mRNA-1:exon:17451;Parent=Msa1201260-mRNA-1
chr8_1 exon 54446792 54447264 54446792 ID=Msa1201260-mRNA-1:exon:17452;Parent=Msa1201260-mRNA-1
chr8_1 CDS 54446397 54446651 54446397 ID=Msa1201260-mRNA-1:cds;Parent=Msa1201260-mRNA-1
chr8_1 CDS 54446792 54446965 54446792 ID=Msa1201260-mRNA-1:cds;Parent=Msa1201260-mRNA-1
chr8_1 three_prime_UTR 54446966 54447264 54446966 ID=Msa1201260-mRNA-1:three_prime_utr;Parent=Msa1201260-mRNA-1
Gene Sequence

>Msa1201260

ATGGGTGTATCCAAGAAATGGTTGGGAATCGTTAGAAAAAAATTCCTAAGATCATCCAAAAAAGACATAACTCTCCATCCATGTATAAGTGTTTGCACAAATCAAAGCCAACAAATCATATTTGGAAATGAATCAACCACCACCAGAGAAGATTTACTCAGCTTGCCACCACCACATATTAATGCTTCATTGATCATATTCAACAAAGAAGACTTTGCAGCAATTAAGATTCAAGCTTATTTCAGGGGTCATCTCGCAAGAAGAGCACATAGGGCATTGAAAAGCCTTGTGAAATTGCAAGCATTGGTTCGCGGGGTGTGTGTGAGGAGACAATCGCGTATAGCAATGCAGTGTATGCACGCACTTGTTCGATTACAAGTCAAGGTTCGTGCAAGGCAATTACTCGGTAGCTTTGATCATAGTCGATAA

Protein sequence

>Msa1201260

MGVSKKWLGIVRKKFLRSSKKDITLHPCISVCTNQSQQIIFGNESTTTREDLLSLPPPHINASLIIFNKEDFAAIKIQAYFRGHLARRAHRALKSLVKLQALVRGVCVRRQSRIAMQCMHALVRLQVKVRARQLLGSFDHSR*