AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1201420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201420 MtrunA17_Chr8g0371391 95.522 201 8 1 1 200 1 201 1.70e-137 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201420 AT5G66090.1 66.258 163 49 2 43 200 49 210 2.02e-75 226

Find 86 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATATATTGTAATCAATTT+TGG 0.136663 8_1:+54616657 Msa1201420:three_prime_UTR
ATATGCTACTCTAAAGTTTC+AGG 0.183780 8_1:+54616322 Msa1201420:three_prime_UTR
TGTTGCCGATGAACTTAATT+TGG 0.184303 8_1:+54616129 Msa1201420:CDS
GTGGTTAACTTCTCTGAGTT+TGG 0.240799 8_1:-54612852 None:intergenic
TGGTTAACTTCTCTGAGTTT+GGG 0.250493 8_1:-54612851 None:intergenic
TTGAAGAATAGGGAGGGTTT+TGG 0.277998 8_1:-54612937 None:intergenic
AAACTCATCATTTCCTTTCC+AGG 0.296477 8_1:-54616428 None:intergenic
CTGAAGCATGACTAATATTT+AGG 0.306734 8_1:+54616405 Msa1201420:three_prime_UTR
TTTAGTTCCCTCAGAGAGAT+AGG 0.318311 8_1:-54613325 None:intergenic
GAGTTTGTGAGGAGTGGATT+TGG 0.319040 8_1:+54616445 Msa1201420:three_prime_UTR
CGATGAACTTAATTTGGATT+TGG 0.322860 8_1:+54616135 Msa1201420:CDS
GAGGGTGTGAAGTCGGGAAG+GGG 0.335959 8_1:-54612998 None:intergenic
AGGGTGTGAAGTCGGGAAGG+GGG 0.337342 8_1:-54612997 None:intergenic
AATTATACACAACCTAAAAT+AGG 0.338514 8_1:-54616522 None:intergenic
AGTAAGCCAACAGCAATAAT+TGG 0.349886 8_1:+54616103 Msa1201420:CDS
TCAAAACACTAATGTTGGTT+TGG 0.369198 8_1:+54616569 Msa1201420:three_prime_UTR
TTTATCAATTTCGTTGTTTG+AGG 0.383976 8_1:-54612785 None:intergenic
GGGTGTGAAGTCGGGAAGGG+GGG 0.386173 8_1:-54612996 None:intergenic
TGGGTGTGTTATTGAAGAAT+AGG 0.393378 8_1:-54612948 None:intergenic
TTTGGGGAAGAGTGATGAAC+AGG 0.400374 8_1:-54612834 None:intergenic
GGTGGGGAGGGTGTGAAGTC+GGG 0.401871 8_1:-54613004 None:intergenic
AGATAAATGCCAACGGATGC+TGG 0.406698 8_1:-54614242 None:intergenic
ATTGCCTTAGCTAACAAGTA+TGG 0.418236 8_1:+54614018 Msa1201420:CDS
ACCCTCCCCACCAGGATCAT+TGG 0.426256 8_1:+54613015 Msa1201420:CDS
TCTCTCTGAGGGAACTAAAC+AGG 0.436554 8_1:+54613329 Msa1201420:CDS
GGGTGTGTTATTGAAGAATA+GGG 0.438335 8_1:-54612947 None:intergenic
GATAGTTGGGTCTCGTGCTC+AGG 0.444353 8_1:-54614220 None:intergenic
TCAGTAGCTACAGCAACTTC+AGG 0.444631 8_1:-54613252 None:intergenic
AAGTGGGAGGTGATGGAAAT+GGG 0.445737 8_1:-54612967 None:intergenic
TACAAATTGCTTGAGCGGCT+TGG 0.450205 8_1:+54616073 Msa1201420:CDS
GCCAACGGATGCTGGATAGT+TGG 0.451723 8_1:-54614234 None:intergenic
CCAACGGATGCTGGATAGTT+GGG 0.456468 8_1:-54614233 None:intergenic
GGGAGGGTGTGAAGTCGGGA+AGG 0.458649 8_1:-54613000 None:intergenic
GGAGGGTGTGAAGTCGGGAA+GGG 0.463065 8_1:-54612999 None:intergenic
TTGCTTGAGCGGCTTGGTGA+TGG 0.467825 8_1:+54616079 Msa1201420:CDS
ATTATTCAAAACACTAATGT+TGG 0.468386 8_1:+54616564 Msa1201420:three_prime_UTR
AAAGTGGGAGGTGATGGAAA+TGG 0.468946 8_1:-54612968 None:intergenic
GGAGCTGACATTACAGTTGT+AGG 0.469978 8_1:+54614039 Msa1201420:CDS
TGGCAAAAGCATGACAGGAC+AGG 0.472545 8_1:-54616224 None:intergenic
ACCAATGATCCTGGTGGGGA+GGG 0.491868 8_1:-54613016 None:intergenic
ACATCGCCAATTATTGCTGT+TGG 0.493106 8_1:-54616109 None:intergenic
ATCCGTTGGCATTTATCTGA+AGG 0.506635 8_1:+54614247 Msa1201420:CDS
TATGCTACTCTAAAGTTTCA+GGG 0.507896 8_1:+54616323 Msa1201420:three_prime_UTR
TGGTGGGGAGGGTGTGAAGT+CGG 0.513130 8_1:-54613005 None:intergenic
GACTTCACACCCTCCCCACC+AGG 0.519206 8_1:+54613007 Msa1201420:CDS
TACAATGGCAAAAGCATGAC+AGG 0.519854 8_1:-54616229 None:intergenic
TTAGTTCCCTCAGAGAGATA+GGG 0.523045 8_1:-54613324 None:intergenic
TGCTTGAGCGGCTTGGTGAT+GGG 0.524122 8_1:+54616080 Msa1201420:CDS
CAAATCCAAATTAAGTTCAT+CGG 0.524886 8_1:-54616134 None:intergenic
AATGATGAGTTTGTGAGGAG+TGG 0.527912 8_1:+54616439 Msa1201420:three_prime_UTR
AATTGATAAAACGAAACCCT+TGG 0.531424 8_1:+54612798 Msa1201420:five_prime_UTR
AGTTATATTACCAATGATCC+TGG 0.538740 8_1:-54613025 None:intergenic
AGCTCCATACTTGTTAGCTA+AGG 0.544707 8_1:-54614022 None:intergenic
TGCATGACTGTACTTGACTA+AGG 0.547431 8_1:+54616361 Msa1201420:three_prime_UTR
GTGTTATTGAAGAATAGGGA+GGG 0.550609 8_1:-54612943 None:intergenic
ACAGCAACTTCAGGTTCTTG+TGG 0.551790 8_1:-54613243 None:intergenic
ACTAATATTTAGGCCTGGAA+AGG 0.553070 8_1:+54616415 Msa1201420:three_prime_UTR
AAGTTAACCACAAGCATCCA+TGG 0.554780 8_1:+54612864 Msa1201420:exon
GCATGACTAATATTTAGGCC+TGG 0.555476 8_1:+54616410 Msa1201420:three_prime_UTR
ATATTACCAATGATCCTGGT+GGG 0.557905 8_1:-54613021 None:intergenic
CTACTAGTATCAAACAATGT+GGG 0.560265 8_1:+54616496 Msa1201420:three_prime_UTR
GGTTAACTTCTCTGAGTTTG+GGG 0.561595 8_1:-54612850 None:intergenic
TACCAATGATCCTGGTGGGG+AGG 0.564673 8_1:-54613017 None:intergenic
GGATTTGGTAGTTATTAGCA+TGG 0.566741 8_1:+54616150 Msa1201420:CDS
AGGATTCTAGTGACTTCCAA+GGG 0.574687 8_1:-54612814 None:intergenic
CAGGATTCTAGTGACTTCCA+AGG 0.578238 8_1:-54612815 None:intergenic
AGGACTACAAATTGCTTGAG+CGG 0.586994 8_1:+54616068 Msa1201420:CDS
CTACAAAATGCGAACTACAA+TGG 0.587815 8_1:-54616244 None:intergenic
CCCAACTATCCAGCATCCGT+TGG 0.587965 8_1:+54614233 Msa1201420:CDS
GGCAAAAGCATGACAGGACA+GGG 0.588085 8_1:-54616223 None:intergenic
TATACACAACCTAAAATAGG+AGG 0.600243 8_1:-54616519 None:intergenic
AGAAAACCCTATCTCTCTGA+GGG 0.601139 8_1:+54613318 Msa1201420:CDS
GATTTGGATTCTTTCATGTG+AGG 0.606825 8_1:+54616461 Msa1201420:three_prime_UTR
AGTTATCGATGAACAGCAGA+AGG 0.615289 8_1:+54614189 Msa1201420:intron
GAGGGTTTGACGAGTTGAAG+CGG 0.621708 8_1:-54612904 None:intergenic
TCTACTAGTATCAAACAATG+TGG 0.623417 8_1:+54616495 Msa1201420:three_prime_UTR
TATTACCAATGATCCTGGTG+GGG 0.632686 8_1:-54613020 None:intergenic
TGGCAGAGTCATGAACGCCA+TGG 0.640926 8_1:-54612881 None:intergenic
AACCTTCAGATAAATGCCAA+CGG 0.643797 8_1:-54614249 None:intergenic
ATACTTGTTAGCTAAGGCAA+TGG 0.644889 8_1:-54614016 None:intergenic
TGTGTTATTGAAGAATAGGG+AGG 0.650429 8_1:-54612944 None:intergenic
TAGAAAACCCTATCTCTCTG+AGG 0.651248 8_1:+54613317 Msa1201420:CDS
TATATTACCAATGATCCTGG+TGG 0.656745 8_1:-54613022 None:intergenic
AAGGAAATGATGAGTTTGTG+AGG 0.675607 8_1:+54616434 Msa1201420:three_prime_UTR
GGTTTGACGAGTTGAAGCGG+TGG 0.698692 8_1:-54612901 None:intergenic
ATGAACGCCATGGATGCTTG+TGG 0.717650 8_1:-54612871 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATTATCTTTTTGTT+AGG + chr8_1:54613981-54614000 Msa1201420:intron 10.0%
!!! TATGTAAATATTTTTGTTAT+TGG + chr8_1:54615256-54615275 Msa1201420:intron 10.0%
!!! TAGTAATTTTACGTAATTTA+TGG - chr8_1:54612728-54612747 None:intergenic 15.0%
!! AAGAAACTATAATAATTTCT+TGG - chr8_1:54613077-54613096 None:intergenic 15.0%
!!! CTTTTTCTTTTCAATTTATT+TGG + chr8_1:54613357-54613376 Msa1201420:intron 15.0%
!! TAAGAATTTAGATTCTATAT+GGG - chr8_1:54613618-54613637 None:intergenic 15.0%
!! TTAAGAATTTAGATTCTATA+TGG - chr8_1:54613619-54613638 None:intergenic 15.0%
!! AAATTCATAATATCAAAAAG+AGG - chr8_1:54614093-54614112 None:intergenic 15.0%
!!! TGATATTATGAATTTTTTAC+TGG + chr8_1:54614098-54614117 Msa1201420:intron 15.0%
!!! GATATTATGAATTTTTTACT+GGG + chr8_1:54614099-54614118 Msa1201420:intron 15.0%
!!! ATATTATGAATTTTTTACTG+GGG + chr8_1:54614100-54614119 Msa1201420:intron 15.0%
!!! ATTTTTTCTTTTTAAAAGGT+TGG + chr8_1:54615692-54615711 Msa1201420:intron 15.0%
!! TATTTAATAATGAAAAATGC+AGG + chr8_1:54616037-54616056 Msa1201420:intron 15.0%
!!! CTATATATTGTAATCAATTT+TGG + chr8_1:54616657-54616676 Msa1201420:three_prime_UTR 15.0%
!!! TTGATTTTGTAGAAGTTTTT+AGG - chr8_1:54613805-54613824 None:intergenic 20.0%
!!! TTTTTTTGCTTCTCTTTTTT+TGG + chr8_1:54615226-54615245 Msa1201420:intron 20.0%
!!! CCAATGTTTTATTCAAAAAA+CGG - chr8_1:54615790-54615809 None:intergenic 20.0%
!!! CATTTTTAAGTAAACATTCA+TGG + chr8_1:54615948-54615967 Msa1201420:intron 20.0%
!! AATTATACACAACCTAAAAT+AGG - chr8_1:54616525-54616544 None:intergenic 20.0%
!! ATTATTCAAAACACTAATGT+TGG + chr8_1:54616564-54616583 Msa1201420:three_prime_UTR 20.0%
! TTTATCAATTTCGTTGTTTG+AGG - chr8_1:54612788-54612807 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAAAC+AGG + chr8_1:54613902-54613921 Msa1201420:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + chr8_1:54613903-54613922 Msa1201420:intron 25.0%
!!! ATTTGTTGAGTTTTTTTGTG+TGG + chr8_1:54614297-54614316 Msa1201420:intron 25.0%
! AAACTAGTAAAGTGAAAAGT+AGG + chr8_1:54614356-54614375 Msa1201420:intron 25.0%
! TTATTTGTAATGTACCTTCA+AGG - chr8_1:54614508-54614527 None:intergenic 25.0%
!! AATCTAATAGTGGAAAAAAG+TGG - chr8_1:54614862-54614881 None:intergenic 25.0%
! TGGATGATTTAATCTAATAG+TGG - chr8_1:54614872-54614891 None:intergenic 25.0%
! ATTTGCTCATTGTAAAAAAC+AGG - chr8_1:54615078-54615097 None:intergenic 25.0%
!!! TAGAGTTGATGAGTTAAATT+GGG + chr8_1:54615341-54615360 Msa1201420:intron 25.0%
! TAATATGACAGTGTAAAAAG+TGG + chr8_1:54615400-54615419 Msa1201420:intron 25.0%
!!! TTTTAAGGGTCCATTTTTTA+TGG + chr8_1:54615441-54615460 Msa1201420:intron 25.0%
!!! TTTAAGGGTCCATTTTTTAT+GGG + chr8_1:54615442-54615461 Msa1201420:intron 25.0%
!! ATTGAGTAACAGATTTTAAG+TGG + chr8_1:54615723-54615742 Msa1201420:intron 25.0%
! ATAAGACATAGTAAAGGATA+AGG - chr8_1:54615760-54615779 None:intergenic 25.0%
!!! CCGTTTTTTGAATAAAACAT+TGG + chr8_1:54615787-54615806 Msa1201420:intron 25.0%
!!! TTTGATTTGCTACAAAATGA+AGG - chr8_1:54615838-54615857 None:intergenic 25.0%
! TTAATAATGAAAAATGCAGG+TGG + chr8_1:54616040-54616059 Msa1201420:intron 25.0%
! TAATAATGAAAAATGCAGGT+GGG + chr8_1:54616041-54616060 Msa1201420:intron 25.0%
! CAAATCCAAATTAAGTTCAT+CGG - chr8_1:54616137-54616156 None:intergenic 25.0%
AATTGATAAAACGAAACCCT+TGG + chr8_1:54612798-54612817 Msa1201420:five_prime_UTR 30.0%
AGTTATATTACCAATGATCC+TGG - chr8_1:54613028-54613047 None:intergenic 30.0%
TTTCTTTGATGCATGCAATT+TGG + chr8_1:54613091-54613110 Msa1201420:intron 30.0%
TTCTTTGATGCATGCAATTT+GGG + chr8_1:54613092-54613111 Msa1201420:intron 30.0%
GTTTCATCTGAATTCAGTTA+AGG + chr8_1:54613215-54613234 Msa1201420:intron 30.0%
! TAGGGTTTTCTATCAGTAAT+AGG - chr8_1:54613309-54613328 None:intergenic 30.0%
CAATTTCATTAACGAAAGTC+TGG + chr8_1:54613542-54613561 Msa1201420:intron 30.0%
TTATGAACAATGTCATAAGC+TGG - chr8_1:54613721-54613740 None:intergenic 30.0%
! GGTATTTTCATAAGCTATCT+TGG - chr8_1:54613784-54613803 None:intergenic 30.0%
GTTCTTGTATGAAGTTGTAA+TGG + chr8_1:54614122-54614141 Msa1201420:intron 30.0%
!!! TGAGTTTTTTTGTGTGGATT+TGG + chr8_1:54614303-54614322 Msa1201420:intron 30.0%
! CTTTACTAGTTTACTCTCAA+CGG - chr8_1:54614348-54614367 None:intergenic 30.0%
CTCATGATTGCTTATGTATT+TGG + chr8_1:54614401-54614420 Msa1201420:intron 30.0%
ATGATTGCTTATGTATTTGG+AGG + chr8_1:54614404-54614423 Msa1201420:intron 30.0%
!! TCCATTGATTTATTCGTCAA+TGG + chr8_1:54614435-54614454 Msa1201420:intron 30.0%
ACCATTGACGAATAAATCAA+TGG - chr8_1:54614439-54614458 None:intergenic 30.0%
ATGTACACACAGTGAATTAT+CGG + chr8_1:54614786-54614805 Msa1201420:intron 30.0%
ATCATGGATCAATACTTCAA+CGG - chr8_1:54614836-54614855 None:intergenic 30.0%
AGAAAGAAAATATGAGAGAG+AGG - chr8_1:54614957-54614976 None:intergenic 30.0%
ATCAATTTCTACTAGGATTG+TGG + chr8_1:54615177-54615196 Msa1201420:intron 30.0%
!! GTAGAGTTGATGAGTTAAAT+TGG + chr8_1:54615340-54615359 Msa1201420:intron 30.0%
TGAGTTAAATTGGGTTTAGT+TGG + chr8_1:54615350-54615369 Msa1201420:intron 30.0%
GTGTAAAAAGTGGTATATCA+AGG + chr8_1:54615410-54615429 Msa1201420:intron 30.0%
! ATTTTGTCTTGGGTAAGAAA+AGG + chr8_1:54615568-54615587 Msa1201420:intron 30.0%
CATGCAATAAGACATAGTAA+AGG - chr8_1:54615766-54615785 None:intergenic 30.0%
! CGATGAACTTAATTTGGATT+TGG + chr8_1:54616135-54616154 Msa1201420:CDS 30.0%
!! ATATGCTACTCTAAAGTTTC+AGG + chr8_1:54616322-54616341 Msa1201420:three_prime_UTR 30.0%
!! TATGCTACTCTAAAGTTTCA+GGG + chr8_1:54616323-54616342 Msa1201420:three_prime_UTR 30.0%
CTGAAGCATGACTAATATTT+AGG + chr8_1:54616405-54616424 Msa1201420:three_prime_UTR 30.0%
TCTACTAGTATCAAACAATG+TGG + chr8_1:54616495-54616514 Msa1201420:three_prime_UTR 30.0%
CTACTAGTATCAAACAATGT+GGG + chr8_1:54616496-54616515 Msa1201420:three_prime_UTR 30.0%
TATACACAACCTAAAATAGG+AGG - chr8_1:54616522-54616541 None:intergenic 30.0%
! TCAAAACACTAATGTTGGTT+TGG + chr8_1:54616569-54616588 Msa1201420:three_prime_UTR 30.0%
TGGTTAACTTCTCTGAGTTT+GGG - chr8_1:54612854-54612873 None:intergenic 35.0%
! GTGTTATTGAAGAATAGGGA+GGG - chr8_1:54612946-54612965 None:intergenic 35.0%
! TGTGTTATTGAAGAATAGGG+AGG - chr8_1:54612947-54612966 None:intergenic 35.0%
! GGGTGTGTTATTGAAGAATA+GGG - chr8_1:54612950-54612969 None:intergenic 35.0%
! TGGGTGTGTTATTGAAGAAT+AGG - chr8_1:54612951-54612970 None:intergenic 35.0%
ATATTACCAATGATCCTGGT+GGG - chr8_1:54613024-54613043 None:intergenic 35.0%
TATATTACCAATGATCCTGG+TGG - chr8_1:54613025-54613044 None:intergenic 35.0%
ACTGATAAACACCTTGACTT+AGG + chr8_1:54613434-54613453 Msa1201420:intron 35.0%
TAGCTAAATAATGAGCAGCT+TGG + chr8_1:54613648-54613667 Msa1201420:intron 35.0%
TAAATAATGAGCAGCTTGGT+TGG + chr8_1:54613652-54613671 Msa1201420:intron 35.0%
CCTAACATGCAAAACTGTTT+GGG + chr8_1:54613682-54613701 Msa1201420:intron 35.0%
!!! AACCACAAGTCAGTGTTTTT+TGG - chr8_1:54613952-54613971 None:intergenic 35.0%
! ATACTTGTTAGCTAAGGCAA+TGG - chr8_1:54614019-54614038 None:intergenic 35.0%
ATTGCCTTAGCTAACAAGTA+TGG + chr8_1:54614018-54614037 Msa1201420:CDS 35.0%
AACCTTCAGATAAATGCCAA+CGG - chr8_1:54614252-54614271 None:intergenic 35.0%
GTTGATATTACTCGCTTTAG+AGG + chr8_1:54614457-54614476 Msa1201420:intron 35.0%
!!! ACTGTGTCCTTTTTTTGTTG+AGG + chr8_1:54614481-54614500 Msa1201420:intron 35.0%
TCAAGGACCTCAACAAAAAA+AGG - chr8_1:54614491-54614510 None:intergenic 35.0%
!!! TTTTTTGTTGAGGTCCTTGA+AGG + chr8_1:54614491-54614510 Msa1201420:intron 35.0%
!! GTTCAACTACTATCGATTTG+TGG + chr8_1:54614592-54614611 Msa1201420:intron 35.0%
! AGCTAATACTTGGAGCTTTT+GGG + chr8_1:54614686-54614705 Msa1201420:intron 35.0%
ATTACCAAACACGCGATAAA+AGG + chr8_1:54614763-54614782 Msa1201420:intron 35.0%
ACACAGTGAATTATCGGATA+TGG + chr8_1:54614792-54614811 Msa1201420:intron 35.0%
TGGAAAAAAGTGGTCTATCA+TGG - chr8_1:54614852-54614871 None:intergenic 35.0%
CATCCAACCAAACTTTATCA+TGG + chr8_1:54614886-54614905 Msa1201420:intron 35.0%
AGACCATGATAAAGTTTGGT+TGG - chr8_1:54614892-54614911 None:intergenic 35.0%
GGTTAGACCATGATAAAGTT+TGG - chr8_1:54614896-54614915 None:intergenic 35.0%
! CACATGGTATTTCTATGACA+TGG - chr8_1:54615035-54615054 None:intergenic 35.0%
TCATAGAAATACCATGTGCA+AGG + chr8_1:54615037-54615056 Msa1201420:intron 35.0%
ATTACATGCTTGCTTGGTAA+TGG - chr8_1:54615122-54615141 None:intergenic 35.0%
! TTTTGGATTACATGCTTGCT+TGG - chr8_1:54615128-54615147 None:intergenic 35.0%
!!! TGTGGCTTTGGTTTTCATTT+TGG - chr8_1:54615145-54615164 None:intergenic 35.0%
CAGCATCATCAATTTCTACT+AGG + chr8_1:54615170-54615189 Msa1201420:intron 35.0%
AGTTCCTTTCTCTTTCAAAG+TGG - chr8_1:54615295-54615314 None:intergenic 35.0%
AGTTGGACTGTGATAAGATT+TGG + chr8_1:54615367-54615386 Msa1201420:intron 35.0%
! ATCAAGGACCAGTCTTTTTA+AGG + chr8_1:54615426-54615445 Msa1201420:intron 35.0%
! TCAAGGACCAGTCTTTTTAA+GGG + chr8_1:54615427-54615446 Msa1201420:intron 35.0%
AAATGGACCCTTAAAAAGAC+TGG - chr8_1:54615437-54615456 None:intergenic 35.0%
ACACCAGATATCATATCCTA+GGG - chr8_1:54615468-54615487 None:intergenic 35.0%
AACACCAGATATCATATCCT+AGG - chr8_1:54615469-54615488 None:intergenic 35.0%
!! CCATGGCATTATTTTGTCTT+GGG + chr8_1:54615558-54615577 Msa1201420:intron 35.0%
TAATAAAATTCATCCCCGAG+TGG - chr8_1:54615651-54615670 None:intergenic 35.0%
! ACTCGGGGATGAATTTTATT+AGG + chr8_1:54615650-54615669 Msa1201420:intron 35.0%
AGTAAGCCAACAGCAATAAT+TGG + chr8_1:54616103-54616122 Msa1201420:CDS 35.0%
TGTTGCCGATGAACTTAATT+TGG + chr8_1:54616129-54616148 Msa1201420:CDS 35.0%
! GGATTTGGTAGTTATTAGCA+TGG + chr8_1:54616150-54616169 Msa1201420:CDS 35.0%
CTACAAAATGCGAACTACAA+TGG - chr8_1:54616247-54616266 None:intergenic 35.0%
! TAGTTCGCATTTTGTAGACA+GGG + chr8_1:54616250-54616269 Msa1201420:exon 35.0%
ACTAATATTTAGGCCTGGAA+AGG + chr8_1:54616415-54616434 Msa1201420:three_prime_UTR 35.0%
AAACTCATCATTTCCTTTCC+AGG - chr8_1:54616431-54616450 None:intergenic 35.0%
AAGGAAATGATGAGTTTGTG+AGG + chr8_1:54616434-54616453 Msa1201420:three_prime_UTR 35.0%
GATTTGGATTCTTTCATGTG+AGG + chr8_1:54616461-54616480 Msa1201420:three_prime_UTR 35.0%
AGGATTCTAGTGACTTCCAA+GGG - chr8_1:54612817-54612836 None:intergenic 40.0%
GGTTAACTTCTCTGAGTTTG+GGG - chr8_1:54612853-54612872 None:intergenic 40.0%
GTGGTTAACTTCTCTGAGTT+TGG - chr8_1:54612855-54612874 None:intergenic 40.0%
AAGTTAACCACAAGCATCCA+TGG + chr8_1:54612864-54612883 Msa1201420:exon 40.0%
!!! GGTTTTGGAGAGATAAGAGA+GGG - chr8_1:54612925-54612944 None:intergenic 40.0%
! TTGAAGAATAGGGAGGGTTT+TGG - chr8_1:54612940-54612959 None:intergenic 40.0%
TATTACCAATGATCCTGGTG+GGG - chr8_1:54613023-54613042 None:intergenic 40.0%
TAGAAAACCCTATCTCTCTG+AGG + chr8_1:54613317-54613336 Msa1201420:CDS 40.0%
AGAAAACCCTATCTCTCTGA+GGG + chr8_1:54613318-54613337 Msa1201420:CDS 40.0%
TTAGTTCCCTCAGAGAGATA+GGG - chr8_1:54613327-54613346 None:intergenic 40.0%
! TTTAGTTCCCTCAGAGAGAT+AGG - chr8_1:54613328-54613347 None:intergenic 40.0%
! ACCTTGACTTAGGACTTGTT+TGG + chr8_1:54613444-54613463 Msa1201420:intron 40.0%
TCCAAACAAGTCCTAAGTCA+AGG - chr8_1:54613448-54613467 None:intergenic 40.0%
TAAGCACTTGTGAGACTGTT+TGG + chr8_1:54613483-54613502 Msa1201420:intron 40.0%
AAGCACTTGTGAGACTGTTT+GGG + chr8_1:54613484-54613503 Msa1201420:intron 40.0%
AAGACTGCAGAAATACAAGG+CGG + chr8_1:54613581-54613600 Msa1201420:intron 40.0%
GCCTAACATGCAAAACTGTT+TGG + chr8_1:54613681-54613700 Msa1201420:intron 40.0%
! CCCAAACAGTTTTGCATGTT+AGG - chr8_1:54613685-54613704 None:intergenic 40.0%
CACAAAATTAACGCCCTGTT+TGG - chr8_1:54613919-54613938 None:intergenic 40.0%
!! CACCAAAAAACACTGACTTG+TGG + chr8_1:54613947-54613966 Msa1201420:intron 40.0%
!! AAAACACTGACTTGTGGTTG+TGG + chr8_1:54613953-54613972 Msa1201420:intron 40.0%
! AGCTCCATACTTGTTAGCTA+AGG - chr8_1:54614025-54614044 None:intergenic 40.0%
AGTTATCGATGAACAGCAGA+AGG + chr8_1:54614189-54614208 Msa1201420:intron 40.0%
ATCCGTTGGCATTTATCTGA+AGG + chr8_1:54614247-54614266 Msa1201420:CDS 40.0%
TTAAGAGCCAAGTGTGTGTT+AGG + chr8_1:54614640-54614659 Msa1201420:intron 40.0%
TAAGAGCCAAGTGTGTGTTA+GGG + chr8_1:54614641-54614660 Msa1201420:intron 40.0%
ATTAGCTCCAACCTACTTTG+CGG - chr8_1:54614673-54614692 None:intergenic 40.0%
! AGTAGGTTGGAGCTAATACT+TGG + chr8_1:54614676-54614695 Msa1201420:intron 40.0%
! GAGCTAATACTTGGAGCTTT+TGG + chr8_1:54614685-54614704 Msa1201420:intron 40.0%
!! TTGGAGCTTTTGGGAAATTG+CGG + chr8_1:54614695-54614714 Msa1201420:intron 40.0%
GAAGCTTAAACATGAGGTAG+AGG + chr8_1:54614724-54614743 Msa1201420:intron 40.0%
! ACATCCTTTTATCGCGTGTT+TGG - chr8_1:54614770-54614789 None:intergenic 40.0%
! ATCGGATATGGAGTCCTTTT+AGG + chr8_1:54614804-54614823 Msa1201420:intron 40.0%
!! GATATGGAGTCCTTTTAGGT+TGG + chr8_1:54614808-54614827 Msa1201420:intron 40.0%
TTCAACGGATCCAACCTAAA+AGG - chr8_1:54614821-54614840 None:intergenic 40.0%
ATGAGAGAGATGGAGTCAAT+GGG - chr8_1:54614932-54614951 None:intergenic 40.0%
AATGAGAGAGATGGAGTCAA+TGG - chr8_1:54614933-54614952 None:intergenic 40.0%
AGCATAGCAGTAAAGGGATT+GGG - chr8_1:54615010-54615029 None:intergenic 40.0%
! ATGGAGAGCATAGCAGTAAA+GGG - chr8_1:54615016-54615035 None:intergenic 40.0%
TGAGCAAATCCAAAACCGAT+AGG + chr8_1:54615089-54615108 Msa1201420:intron 40.0%
!! ATTGATGATGCTGTCAACTG+TGG - chr8_1:54615163-54615182 None:intergenic 40.0%
AGGATTGTGGCATCATTTGA+AGG + chr8_1:54615190-54615209 Msa1201420:intron 40.0%
! TCAGCCACTTTGAAAGAGAA+AGG + chr8_1:54615288-54615307 Msa1201420:intron 40.0%
TGGACTGTGATAAGATTTGG+TGG + chr8_1:54615370-54615389 Msa1201420:intron 40.0%
! GGACTGTGATAAGATTTGGT+GGG + chr8_1:54615371-54615390 Msa1201420:intron 40.0%
ATCCTAGGGCCCATAAAAAA+TGG - chr8_1:54615454-54615473 None:intergenic 40.0%
CCATGGCTCAATTACTTGTA+TGG - chr8_1:54615544-54615563 None:intergenic 40.0%
CCATACAAGTAATTGAGCCA+TGG + chr8_1:54615541-54615560 Msa1201420:intron 40.0%
!! GCCATGGCATTATTTTGTCT+TGG + chr8_1:54615557-54615576 Msa1201420:intron 40.0%
CCCAAGACAAAATAATGCCA+TGG - chr8_1:54615561-54615580 None:intergenic 40.0%
TCAAATGCACTGTCTGTGTT+AGG + chr8_1:54615853-54615872 Msa1201420:intron 40.0%
GGTATATAAGTGTGCGCTTA+AGG + chr8_1:54615969-54615988 Msa1201420:intron 40.0%
AGGACTACAAATTGCTTGAG+CGG + chr8_1:54616068-54616087 Msa1201420:CDS 40.0%
ACATCGCCAATTATTGCTGT+TGG - chr8_1:54616112-54616131 None:intergenic 40.0%
! TACAATGGCAAAAGCATGAC+AGG - chr8_1:54616232-54616251 None:intergenic 40.0%
! GTAGTTCGCATTTTGTAGAC+AGG + chr8_1:54616249-54616268 Msa1201420:exon 40.0%
TGCATGACTGTACTTGACTA+AGG + chr8_1:54616361-54616380 Msa1201420:three_prime_UTR 40.0%
GCATGACTAATATTTAGGCC+TGG + chr8_1:54616410-54616429 Msa1201420:three_prime_UTR 40.0%
AATGATGAGTTTGTGAGGAG+TGG + chr8_1:54616439-54616458 Msa1201420:three_prime_UTR 40.0%
CAGGATTCTAGTGACTTCCA+AGG - chr8_1:54612818-54612837 None:intergenic 45.0%
TTTGGGGAAGAGTGATGAAC+AGG - chr8_1:54612837-54612856 None:intergenic 45.0%
!!! GGGTTTTGGAGAGATAAGAG+AGG - chr8_1:54612926-54612945 None:intergenic 45.0%
!! AAGTGGGAGGTGATGGAAAT+GGG - chr8_1:54612970-54612989 None:intergenic 45.0%
!! AAAGTGGGAGGTGATGGAAA+TGG - chr8_1:54612971-54612990 None:intergenic 45.0%
GTTGAAAAAGTGGGAGGTGA+TGG - chr8_1:54612977-54612996 None:intergenic 45.0%
! ACAGCAACTTCAGGTTCTTG+TGG - chr8_1:54613246-54613265 None:intergenic 45.0%
TCAGTAGCTACAGCAACTTC+AGG - chr8_1:54613255-54613274 None:intergenic 45.0%
! TCTCTCTGAGGGAACTAAAC+AGG + chr8_1:54613329-54613348 Msa1201420:CDS 45.0%
GCCAAGACTGCAGAAATACA+AGG + chr8_1:54613578-54613597 Msa1201420:intron 45.0%
! GCCTTGTATTTCTGCAGTCT+TGG - chr8_1:54613582-54613601 None:intergenic 45.0%
GGAGCTGACATTACAGTTGT+AGG + chr8_1:54614039-54614058 Msa1201420:CDS 45.0%
AGATAAATGCCAACGGATGC+TGG - chr8_1:54614245-54614264 None:intergenic 45.0%
AAGAGCCAAGTGTGTGTTAG+GGG + chr8_1:54614642-54614661 Msa1201420:intron 45.0%
!! AGCTTTTGGGAAATTGCGGT+GGG + chr8_1:54614699-54614718 Msa1201420:intron 45.0%
TGGGAGGAAGCTTAAACATG+AGG + chr8_1:54614718-54614737 Msa1201420:intron 45.0%
!! TCAATGGGTTAGATCTGGTG+TGG - chr8_1:54614917-54614936 None:intergenic 45.0%
!! TGGAGTCAATGGGTTAGATC+TGG - chr8_1:54614922-54614941 None:intergenic 45.0%
GAGCATAGCAGTAAAGGGAT+TGG - chr8_1:54615011-54615030 None:intergenic 45.0%
! CATGGAGAGCATAGCAGTAA+AGG - chr8_1:54615017-54615036 None:intergenic 45.0%
! GGTAATGGTAGGCTTCCTAT+CGG - chr8_1:54615107-54615126 None:intergenic 45.0%
!! CATGCTTGCTTGGTAATGGT+AGG - chr8_1:54615118-54615137 None:intergenic 45.0%
!!! GTCCATTTTTTATGGGCCCT+AGG + chr8_1:54615449-54615468 Msa1201420:intron 45.0%
GAGTGAGTGAAGAGCAAAAG+AGG + chr8_1:54615595-54615614 Msa1201420:intron 45.0%
TGAAGAGCAAAAGAGGACGA+AGG + chr8_1:54615602-54615621 Msa1201420:intron 45.0%
!! GAAAAATGCAGGTGGGTTGA+AGG + chr8_1:54616048-54616067 Msa1201420:intron 45.0%
TACAAATTGCTTGAGCGGCT+TGG + chr8_1:54616073-54616092 Msa1201420:CDS 45.0%
! GAGTTTGTGAGGAGTGGATT+TGG + chr8_1:54616445-54616464 Msa1201420:three_prime_UTR 45.0%
! CAATGTGGGCCTCCTATTTT+AGG + chr8_1:54616510-54616529 Msa1201420:three_prime_UTR 45.0%
!!! ATTTATTTTTTCTTTTTAAA+AGG + chr8_1:54615688-54615707 Msa1201420:intron 5.0%
ATGAACGCCATGGATGCTTG+TGG - chr8_1:54612874-54612893 None:intergenic 50.0%
! GAGGGTTTGACGAGTTGAAG+CGG - chr8_1:54612907-54612926 None:intergenic 50.0%
GGAAGGGGGGTTGAAAAAGT+GGG - chr8_1:54612986-54613005 None:intergenic 50.0%
GCTATCTTGGACAGTCTCAC+AGG - chr8_1:54613771-54613790 None:intergenic 50.0%
CCAACGGATGCTGGATAGTT+GGG - chr8_1:54614236-54614255 None:intergenic 50.0%
TCACACCCCTAACACACACT+TGG - chr8_1:54614650-54614669 None:intergenic 50.0%
!! GAGCTTTTGGGAAATTGCGG+TGG + chr8_1:54614698-54614717 Msa1201420:intron 50.0%
!! TTTTGGGAAATTGCGGTGGG+AGG + chr8_1:54614702-54614721 Msa1201420:intron 50.0%
! CGCGTGTTTGGTAATCCACT+GGG - chr8_1:54614758-54614777 None:intergenic 50.0%
! TCGCGTGTTTGGTAATCCAC+TGG - chr8_1:54614759-54614778 None:intergenic 50.0%
GAGAGAGGCAATGAGAGAGA+TGG - chr8_1:54614942-54614961 None:intergenic 50.0%
ACTTGCTTCAGCCTTGCACA+TGG - chr8_1:54615051-54615070 None:intergenic 50.0%
!!! GGTAGGCTTCCTATCGGTTT+TGG - chr8_1:54615101-54615120 None:intergenic 50.0%
GATGCTGTCAACTGTGGCTT+TGG - chr8_1:54615157-54615176 None:intergenic 50.0%
GGGCCCTAGGATATGATATC+TGG + chr8_1:54615462-54615481 Msa1201420:intron 50.0%
AGAGCAAAAGAGGACGAAGG+AGG + chr8_1:54615605-54615624 Msa1201420:intron 50.0%
CAAAAGAGGACGAAGGAGGA+AGG + chr8_1:54615609-54615628 Msa1201420:intron 50.0%
AGGAAGGACGAGGGAGATAA+GGG + chr8_1:54615625-54615644 Msa1201420:intron 50.0%
! GGCAAAAGCATGACAGGACA+GGG - chr8_1:54616226-54616245 None:intergenic 50.0%
! TGGCAAAAGCATGACAGGAC+AGG - chr8_1:54616227-54616246 None:intergenic 50.0%
TGGCAGAGTCATGAACGCCA+TGG - chr8_1:54612884-54612903 None:intergenic 55.0%
! GGTTTGACGAGTTGAAGCGG+TGG - chr8_1:54612904-54612923 None:intergenic 55.0%
AGGGGGGTTGAAAAAGTGGG+AGG - chr8_1:54612983-54613002 None:intergenic 55.0%
GGGAAGGGGGGTTGAAAAAG+TGG - chr8_1:54612987-54613006 None:intergenic 55.0%
ACCAATGATCCTGGTGGGGA+GGG - chr8_1:54613019-54613038 None:intergenic 55.0%
TACCAATGATCCTGGTGGGG+AGG - chr8_1:54613020-54613039 None:intergenic 55.0%
GATAGTTGGGTCTCGTGCTC+AGG - chr8_1:54614223-54614242 None:intergenic 55.0%
CCCAACTATCCAGCATCCGT+TGG + chr8_1:54614233-54614252 Msa1201420:CDS 55.0%
GCCAACGGATGCTGGATAGT+TGG - chr8_1:54614237-54614256 None:intergenic 55.0%
! TAGGGGTGTGACCGCAAAGT+AGG + chr8_1:54614659-54614678 Msa1201420:intron 55.0%
GGTGTGACCGCAAAGTAGGT+TGG + chr8_1:54614663-54614682 Msa1201420:intron 55.0%
GTAGAGGTGATGTGACCCAG+TGG + chr8_1:54614740-54614759 Msa1201420:intron 55.0%
GCAGTAAAGGGATTGGGAGC+AGG - chr8_1:54615004-54615023 None:intergenic 55.0%
GAGGAAGGACGAGGGAGATA+AGG + chr8_1:54615624-54615643 Msa1201420:intron 55.0%
!! TTGCTTGAGCGGCTTGGTGA+TGG + chr8_1:54616079-54616098 Msa1201420:CDS 55.0%
!! TGCTTGAGCGGCTTGGTGAT+GGG + chr8_1:54616080-54616099 Msa1201420:CDS 55.0%
AGGGTGTGAAGTCGGGAAGG+GGG - chr8_1:54613000-54613019 None:intergenic 60.0%
! GAGGGTGTGAAGTCGGGAAG+GGG - chr8_1:54613001-54613020 None:intergenic 60.0%
! GGAGGGTGTGAAGTCGGGAA+GGG - chr8_1:54613002-54613021 None:intergenic 60.0%
! TGGTGGGGAGGGTGTGAAGT+CGG - chr8_1:54613008-54613027 None:intergenic 60.0%
ACCCTCCCCACCAGGATCAT+TGG + chr8_1:54613015-54613034 Msa1201420:CDS 60.0%
AGGACGAAGGAGGAAGGACG+AGG + chr8_1:54615615-54615634 Msa1201420:intron 60.0%
GGACGAAGGAGGAAGGACGA+GGG + chr8_1:54615616-54615635 Msa1201420:intron 60.0%
CGAGGGAGATAAGGGCCACT+CGG + chr8_1:54615633-54615652 Msa1201420:intron 60.0%
GAGGGAGATAAGGGCCACTC+GGG + chr8_1:54615634-54615653 Msa1201420:intron 60.0%
AGGGAGATAAGGGCCACTCG+GGG + chr8_1:54615635-54615654 Msa1201420:intron 60.0%
GGGTGTGAAGTCGGGAAGGG+GGG - chr8_1:54612999-54613018 None:intergenic 65.0%
! GGGAGGGTGTGAAGTCGGGA+AGG - chr8_1:54613003-54613022 None:intergenic 65.0%
! GGTGGGGAGGGTGTGAAGTC+GGG - chr8_1:54613007-54613026 None:intergenic 65.0%
GACTTCACACCCTCCCCACC+AGG + chr8_1:54613007-54613026 Msa1201420:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 54612698 54616688 54612698 ID=Msa1201420;Name=Msa1201420
chr8_1 mRNA 54612698 54616688 54612698 ID=Msa1201420-mRNA-1;Parent=Msa1201420;Name=Msa1201420-mRNA-1;_AED=0.17;_eAED=0.17;_QI=185|1|1|1|1|1|5|436|200
chr8_1 exon 54612698 54613036 54612698 ID=Msa1201420-mRNA-1:exon:17599;Parent=Msa1201420-mRNA-1
chr8_1 exon 54613232 54613350 54613232 ID=Msa1201420-mRNA-1:exon:17600;Parent=Msa1201420-mRNA-1
chr8_1 exon 54614003 54614060 54614003 ID=Msa1201420-mRNA-1:exon:17601;Parent=Msa1201420-mRNA-1
chr8_1 exon 54614191 54614268 54614191 ID=Msa1201420-mRNA-1:exon:17602;Parent=Msa1201420-mRNA-1
chr8_1 exon 54616059 54616688 54616059 ID=Msa1201420-mRNA-1:exon:17603;Parent=Msa1201420-mRNA-1
chr8_1 five_prime_UTR 54612698 54612882 54612698 ID=Msa1201420-mRNA-1:five_prime_utr;Parent=Msa1201420-mRNA-1
chr8_1 CDS 54612883 54613036 54612883 ID=Msa1201420-mRNA-1:cds;Parent=Msa1201420-mRNA-1
chr8_1 CDS 54613232 54613350 54613232 ID=Msa1201420-mRNA-1:cds;Parent=Msa1201420-mRNA-1
chr8_1 CDS 54614003 54614060 54614003 ID=Msa1201420-mRNA-1:cds;Parent=Msa1201420-mRNA-1
chr8_1 CDS 54614191 54614268 54614191 ID=Msa1201420-mRNA-1:cds;Parent=Msa1201420-mRNA-1
chr8_1 CDS 54616059 54616252 54616059 ID=Msa1201420-mRNA-1:cds;Parent=Msa1201420-mRNA-1
chr8_1 three_prime_UTR 54616253 54616688 54616253 ID=Msa1201420-mRNA-1:three_prime_utr;Parent=Msa1201420-mRNA-1
Gene Sequence

>Msa1201420

ATGGCGTTCATGACTCTGCCACCGCTTCAACTCGTCAAACCCTCTCTTATCTCTCCAAAACCCTCCCTATTCTTCAATAACACACCCATTTCCATCACCTCCCACTTTTTCAACCCCCCTTCCCGACTTCACACCCTCCCCACCAGGATCATTGTTAAGGCTAAACCACAAGAACCTGAAGTTGCTGTAGCTACTGATTCATTTACACAATTCAATCATCTTCTTTTGCCTATTACTGATAGAAAACCCTATCTCTCTGAGGGAACTAAACAGGCTATAGCGACTACCATTGCCTTAGCTAACAAGTATGGAGCTGACATTACAGTTGTAGTTATCGATGAACAGCAGAAGGAGTCACTGCCTGAGCACGAGACCCAACTATCCAGCATCCGTTGGCATTTATCTGAAGGTGGGTTGAAGGACTACAAATTGCTTGAGCGGCTTGGTGATGGGAGTAAGCCAACAGCAATAATTGGCGATGTTGCCGATGAACTTAATTTGGATTTGGTAGTTATTAGCATGGAAGCAATTCATACAAAGCATATAGATGCAAATTTGCTTGCTGAGTTCATTCCCTGTCCTGTCATGCTTTTGCCATTGTAG
Protein sequence

>Msa1201420

MAFMTLPPLQLVKPSLISPKPSLFFNNTPISITSHFFNPPSRLHTLPTRIIVKAKPQEPEVAVATDSFTQFNHLLLPITDRKPYLSEGTKQAIATTIALANKYGADITVVVIDEQQKESLPEHETQLSSIRWHLSEGGLKDYKLLERLGDGSKPTAIIGDVADELNLDLVVISMEAIHTKHIDANLLAEFIPCPVMLLPL*