AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1249630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1249630 MtrunA17_Chr8g0371391 95.522 201 8 1 1 200 1 201 1.70e-137 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1249630 AT5G66090.1 66.258 163 49 2 43 200 49 210 2.02e-75 226

Find 84 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATATATTGTAATCAATTT+TGG 0.136663 8_2:+44583929 Msa1249630:three_prime_UTR
TGTTGCTGATGAACTTAATT+TGG 0.180753 8_2:+44583401 Msa1249630:CDS
ATATGCTACTCTAAAGTTTC+AGG 0.183780 8_2:+44583594 Msa1249630:three_prime_UTR
TGCGGTTACTTCTCTGAGTT+TGG 0.226890 8_2:-44580126 None:intergenic
TTGAAGAATAGGGAGGGTTT+TGG 0.277998 8_2:-44580210 None:intergenic
AAACTCATCATTTCCTTTCC+AGG 0.296477 8_2:-44583700 None:intergenic
CTGAAGCATGACTAATATTT+AGG 0.306734 8_2:+44583677 Msa1249630:three_prime_UTR
TTTAGTTCCCTCAGAGAGAT+AGG 0.318311 8_2:-44580598 None:intergenic
GAGTTTGTGAGGAGTGGATT+TGG 0.319040 8_2:+44583717 Msa1249630:three_prime_UTR
GCGGTTACTTCTCTGAGTTT+GGG 0.329354 8_2:-44580125 None:intergenic
GAGGGTGTGAAGTCGGGAAG+GGG 0.335959 8_2:-44580271 None:intergenic
AGGGTGTGAAGTCGGGAAGG+GGG 0.337342 8_2:-44580270 None:intergenic
AATTATACACAACCTAAAAT+AGG 0.338514 8_2:-44583794 None:intergenic
AGTAAGCCAACAGCAATAAT+TGG 0.349886 8_2:+44583375 Msa1249630:CDS
ACAAGATTCTAGTGACTTCC+AGG 0.360012 8_2:-44580090 None:intergenic
TCAAAACACTAATGTTGGTT+TGG 0.369198 8_2:+44583841 Msa1249630:three_prime_UTR
TTTATCAATTTCGTTGTTTG+AGG 0.383976 8_2:-44580060 None:intergenic
GGGTGTGAAGTCGGGAAGGG+GGG 0.386173 8_2:-44580269 None:intergenic
TGGGTGTGTTATTGAAGAAT+AGG 0.393378 8_2:-44580221 None:intergenic
GGTGGGGAGGGTGTGAAGTC+GGG 0.401871 8_2:-44580277 None:intergenic
TGATGAACTTAATTTGGATT+TGG 0.404360 8_2:+44583407 Msa1249630:CDS
AGATAAATGCCAACGGATGC+TGG 0.406698 8_2:-44581515 None:intergenic
ATTGCCTTAGCTAACAAGTA+TGG 0.418236 8_2:+44581291 Msa1249630:CDS
ACCCTCCCCACCAGGATCAT+TGG 0.426256 8_2:+44580288 Msa1249630:CDS
TCTCTCTGAGGGAACTAAAC+AGG 0.436554 8_2:+44580602 Msa1249630:CDS
GGGTGTGTTATTGAAGAATA+GGG 0.438335 8_2:-44580220 None:intergenic
TCAGTAGCTACAGCAACTTC+AGG 0.444631 8_2:-44580525 None:intergenic
AAGTGGGAGGTGATGGAAAT+GGG 0.445737 8_2:-44580240 None:intergenic
TACAAATTGCTTGAGCGGCT+TGG 0.450205 8_2:+44583345 Msa1249630:CDS
GCCAACGGATGCTGGATAGT+TGG 0.451723 8_2:-44581507 None:intergenic
CCAACGGATGCTGGATAGTT+GGG 0.456468 8_2:-44581506 None:intergenic
GATAGTTGGGTCTCATGCTC+AGG 0.456507 8_2:-44581493 None:intergenic
GGGAGGGTGTGAAGTCGGGA+AGG 0.458649 8_2:-44580273 None:intergenic
GGAGGGTGTGAAGTCGGGAA+GGG 0.463065 8_2:-44580272 None:intergenic
TTGCTTGAGCGGCTTGGTGA+TGG 0.467825 8_2:+44583351 Msa1249630:CDS
ATTATTCAAAACACTAATGT+TGG 0.468386 8_2:+44583836 Msa1249630:three_prime_UTR
AAAGTGGGAGGTGATGGAAA+TGG 0.468946 8_2:-44580241 None:intergenic
GGAGCTGACATTACAGTTGT+AGG 0.469978 8_2:+44581312 Msa1249630:CDS
TGGCAAAAGCATGACAGGAC+AGG 0.472545 8_2:-44583496 None:intergenic
ACATCGCCAATTATTGCTGT+TGG 0.484894 8_2:-44583381 None:intergenic
ACCAATGATCCTGGTGGGGA+GGG 0.491868 8_2:-44580289 None:intergenic
ATCCGTTGGCATTTATCTGA+AGG 0.506635 8_2:+44581520 Msa1249630:CDS
TATGCTACTCTAAAGTTTCA+GGG 0.507896 8_2:+44583595 Msa1249630:three_prime_UTR
TGGTGGGGAGGGTGTGAAGT+CGG 0.513130 8_2:-44580278 None:intergenic
GACTTCACACCCTCCCCACC+AGG 0.519206 8_2:+44580280 Msa1249630:CDS
TACAATGGCAAAAGCATGAC+AGG 0.519854 8_2:-44583501 None:intergenic
TTAGTTCCCTCAGAGAGATA+GGG 0.523045 8_2:-44580597 None:intergenic
TGCTTGAGCGGCTTGGTGAT+GGG 0.524122 8_2:+44583352 Msa1249630:CDS
AATGATGAGTTTGTGAGGAG+TGG 0.527912 8_2:+44583711 Msa1249630:three_prime_UTR
AGTTATATTACCAATGATCC+TGG 0.538740 8_2:-44580298 None:intergenic
GAAGTAACCGCAAGCATCCA+TGG 0.542093 8_2:+44580137 Msa1249630:exon
AGCTCCATACTTGTTAGCTA+AGG 0.544707 8_2:-44581295 None:intergenic
TGCATGACTGTACTTGACTA+AGG 0.547431 8_2:+44583633 Msa1249630:three_prime_UTR
GTGTTATTGAAGAATAGGGA+GGG 0.550609 8_2:-44580216 None:intergenic
ACAGCAACTTCAGGTTCTTG+TGG 0.551790 8_2:-44580516 None:intergenic
ACTAATATTTAGGCCTGGAA+AGG 0.553070 8_2:+44583687 Msa1249630:three_prime_UTR
GCATGACTAATATTTAGGCC+TGG 0.555476 8_2:+44583682 Msa1249630:three_prime_UTR
CGGTTACTTCTCTGAGTTTG+GGG 0.556267 8_2:-44580124 None:intergenic
ATATTACCAATGATCCTGGT+GGG 0.557905 8_2:-44580294 None:intergenic
CTACTAGTATCAAACAATGT+GGG 0.560265 8_2:+44583768 Msa1249630:three_prime_UTR
TACCAATGATCCTGGTGGGG+AGG 0.564673 8_2:-44580290 None:intergenic
GGATTTGGTAGTTATTAGCA+TGG 0.566741 8_2:+44583422 Msa1249630:CDS
CAAGATTCTAGTGACTTCCA+GGG 0.586511 8_2:-44580089 None:intergenic
AGGACTACAAATTGCTTGAG+CGG 0.586994 8_2:+44583340 Msa1249630:CDS
CTACAAAATGCGAACTACAA+TGG 0.587815 8_2:-44583516 None:intergenic
CCCAACTATCCAGCATCCGT+TGG 0.587965 8_2:+44581506 Msa1249630:CDS
GGCAAAAGCATGACAGGACA+GGG 0.588085 8_2:-44583495 None:intergenic
TATACACAACCTAAAATAGG+AGG 0.600243 8_2:-44583791 None:intergenic
AGAAAACCCTATCTCTCTGA+GGG 0.601139 8_2:+44580591 Msa1249630:CDS
GATTTGGATTCTTTCATGTG+AGG 0.606825 8_2:+44583733 Msa1249630:three_prime_UTR
AGTTATCGATGAACAGCAGA+AGG 0.615289 8_2:+44581462 Msa1249630:intron
GAGGGTTTGACGAGTTGAAG+CGG 0.621708 8_2:-44580177 None:intergenic
TCTACTAGTATCAAACAATG+TGG 0.623417 8_2:+44583767 Msa1249630:three_prime_UTR
TATTACCAATGATCCTGGTG+GGG 0.632686 8_2:-44580293 None:intergenic
TGGCAGAGTCATGAACGCCA+TGG 0.640926 8_2:-44580154 None:intergenic
AACCTTCAGATAAATGCCAA+CGG 0.643797 8_2:-44581522 None:intergenic
ATACTTGTTAGCTAAGGCAA+TGG 0.644889 8_2:-44581289 None:intergenic
AAATTGATAAAACGAAACCC+TGG 0.645385 8_2:+44580072 Msa1249630:five_prime_UTR
TGTGTTATTGAAGAATAGGG+AGG 0.650429 8_2:-44580217 None:intergenic
TAGAAAACCCTATCTCTCTG+AGG 0.651248 8_2:+44580590 Msa1249630:CDS
TATATTACCAATGATCCTGG+TGG 0.656745 8_2:-44580295 None:intergenic
ATGAACGCCATGGATGCTTG+CGG 0.669244 8_2:-44580144 None:intergenic
AAGGAAATGATGAGTTTGTG+AGG 0.675607 8_2:+44583706 Msa1249630:three_prime_UTR
GGTTTGACGAGTTGAAGCGG+TGG 0.698692 8_2:-44580174 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATTATCTTTTTGTT+AGG + chr8_2:44581254-44581273 Msa1249630:intron 10.0%
!!! TATGTAAATATTTTTGTTAT+TGG + chr8_2:44582528-44582547 Msa1249630:intron 10.0%
!!! TAGTAATTTTACGTAATTTA+TGG - chr8_2:44580003-44580022 None:intergenic 15.0%
!! AAGAAACTATAATAATTTCT+TGG - chr8_2:44580350-44580369 None:intergenic 15.0%
!!! CTTTTTCTTTTCAATTTATT+TGG + chr8_2:44580630-44580649 Msa1249630:intron 15.0%
!! TAAGAATTTAGATTCTATAT+GGG - chr8_2:44580891-44580910 None:intergenic 15.0%
!! TTAAGAATTTAGATTCTATA+TGG - chr8_2:44580892-44580911 None:intergenic 15.0%
!! AAATTCATAATATCAAAAAG+AGG - chr8_2:44581366-44581385 None:intergenic 15.0%
!!! TGATATTATGAATTTTTTAC+TGG + chr8_2:44581371-44581390 Msa1249630:intron 15.0%
!!! GATATTATGAATTTTTTACT+GGG + chr8_2:44581372-44581391 Msa1249630:intron 15.0%
!!! ATATTATGAATTTTTTACTG+GGG + chr8_2:44581373-44581392 Msa1249630:intron 15.0%
!!! ATTTTTTCTTTTTAAAAGGT+TGG + chr8_2:44582964-44582983 Msa1249630:intron 15.0%
!! TATTTAATAATGAAAAATGC+AGG + chr8_2:44583309-44583328 Msa1249630:intron 15.0%
!!! CTATATATTGTAATCAATTT+TGG + chr8_2:44583929-44583948 Msa1249630:three_prime_UTR 15.0%
!!! TTGATTTTGTAGAAGTTTTT+AGG - chr8_2:44581078-44581097 None:intergenic 20.0%
!!! TTTTTTTGCTTCTCTTTTTT+TGG + chr8_2:44582498-44582517 Msa1249630:intron 20.0%
!!! CCAATGTTTTATTCAAAAAA+CGG - chr8_2:44583062-44583081 None:intergenic 20.0%
!!! CATTTTTAAGTAAACATTCA+TGG + chr8_2:44583220-44583239 Msa1249630:intron 20.0%
!! AATTATACACAACCTAAAAT+AGG - chr8_2:44583797-44583816 None:intergenic 20.0%
!! ATTATTCAAAACACTAATGT+TGG + chr8_2:44583836-44583855 Msa1249630:three_prime_UTR 20.0%
! TTTATCAATTTCGTTGTTTG+AGG - chr8_2:44580063-44580082 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAAAC+AGG + chr8_2:44581175-44581194 Msa1249630:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + chr8_2:44581176-44581195 Msa1249630:intron 25.0%
!!! ATTTGTTGAGTTTTTTTGTG+TGG + chr8_2:44581570-44581589 Msa1249630:intron 25.0%
! AAACTAGTAAAGTGAAAAGT+AGG + chr8_2:44581629-44581648 Msa1249630:intron 25.0%
! TTATTTGTAATGTACCTTCA+AGG - chr8_2:44581781-44581800 None:intergenic 25.0%
!! AATCTAATAGTGGAAAAAAG+TGG - chr8_2:44582134-44582153 None:intergenic 25.0%
! TGGATGATTTAATCTAATAG+TGG - chr8_2:44582144-44582163 None:intergenic 25.0%
! ATTTGCTCATTGTAAAAAAC+AGG - chr8_2:44582350-44582369 None:intergenic 25.0%
!!! TAGAGTTGATGAGTTAAATT+GGG + chr8_2:44582613-44582632 Msa1249630:intron 25.0%
! TAATATGACAGTGTAAAAAG+TGG + chr8_2:44582672-44582691 Msa1249630:intron 25.0%
!!! TTTTAAGGGTCCATTTTTTA+TGG + chr8_2:44582713-44582732 Msa1249630:intron 25.0%
!!! TTTAAGGGTCCATTTTTTAT+GGG + chr8_2:44582714-44582733 Msa1249630:intron 25.0%
!! ATTGAGTAACAGATTTTAAG+TGG + chr8_2:44582995-44583014 Msa1249630:intron 25.0%
! ATAAGACATAGTAAAGGATA+AGG - chr8_2:44583032-44583051 None:intergenic 25.0%
!!! CCGTTTTTTGAATAAAACAT+TGG + chr8_2:44583059-44583078 Msa1249630:intron 25.0%
!!! TTTGATTTGCTACAAAATGA+AGG - chr8_2:44583110-44583129 None:intergenic 25.0%
! TTAATAATGAAAAATGCAGG+TGG + chr8_2:44583312-44583331 Msa1249630:intron 25.0%
! TAATAATGAAAAATGCAGGT+GGG + chr8_2:44583313-44583332 Msa1249630:intron 25.0%
!! TGATGAACTTAATTTGGATT+TGG + chr8_2:44583407-44583426 Msa1249630:CDS 25.0%
AAATTGATAAAACGAAACCC+TGG + chr8_2:44580072-44580091 Msa1249630:five_prime_UTR 30.0%
AGTTATATTACCAATGATCC+TGG - chr8_2:44580301-44580320 None:intergenic 30.0%
TTTCTTTGATGCATGCAATT+TGG + chr8_2:44580364-44580383 Msa1249630:intron 30.0%
TTCTTTGATGCATGCAATTT+GGG + chr8_2:44580365-44580384 Msa1249630:intron 30.0%
GTTTCATCTGAATTCAGTTA+AGG + chr8_2:44580488-44580507 Msa1249630:intron 30.0%
! TAGGGTTTTCTATCAGTAAT+AGG - chr8_2:44580582-44580601 None:intergenic 30.0%
CAATTTCATTAACGAAAGTC+TGG + chr8_2:44580815-44580834 Msa1249630:intron 30.0%
TTATGAACAATGTCATAAGC+TGG - chr8_2:44580994-44581013 None:intergenic 30.0%
! GGTATTTTCATAAGCTATCT+TGG - chr8_2:44581057-44581076 None:intergenic 30.0%
GTTCTTGTATGAAGTTGTAA+TGG + chr8_2:44581395-44581414 Msa1249630:intron 30.0%
!!! TGAGTTTTTTTGTGTGGATT+TGG + chr8_2:44581576-44581595 Msa1249630:intron 30.0%
! CTTTACTAGTTTACTCTCAA+CGG - chr8_2:44581621-44581640 None:intergenic 30.0%
CTCATGATTGCTTATGTATT+TGG + chr8_2:44581674-44581693 Msa1249630:intron 30.0%
ATGATTGCTTATGTATTTGG+AGG + chr8_2:44581677-44581696 Msa1249630:intron 30.0%
!! TCCATTGATTTATTCGTCAA+TGG + chr8_2:44581708-44581727 Msa1249630:intron 30.0%
ACCATTGACGAATAAATCAA+TGG - chr8_2:44581712-44581731 None:intergenic 30.0%
ATGTACACACAGTGAATTAT+CGG + chr8_2:44582058-44582077 Msa1249630:intron 30.0%
ATCATGGATCAATACTTCAA+CGG - chr8_2:44582108-44582127 None:intergenic 30.0%
AGAAAGAAAATATGAGAGAG+AGG - chr8_2:44582229-44582248 None:intergenic 30.0%
ATCAATTTCTACTAGGATTG+TGG + chr8_2:44582449-44582468 Msa1249630:intron 30.0%
!! GTAGAGTTGATGAGTTAAAT+TGG + chr8_2:44582612-44582631 Msa1249630:intron 30.0%
TGAGTTAAATTGGGTTTAGT+TGG + chr8_2:44582622-44582641 Msa1249630:intron 30.0%
GTGTAAAAAGTGGTATATCA+AGG + chr8_2:44582682-44582701 Msa1249630:intron 30.0%
! ATTTTGTCTTGGGTAAGAAA+AGG + chr8_2:44582840-44582859 Msa1249630:intron 30.0%
CATGCAATAAGACATAGTAA+AGG - chr8_2:44583038-44583057 None:intergenic 30.0%
TGTTGCTGATGAACTTAATT+TGG + chr8_2:44583401-44583420 Msa1249630:CDS 30.0%
!! ATATGCTACTCTAAAGTTTC+AGG + chr8_2:44583594-44583613 Msa1249630:three_prime_UTR 30.0%
!! TATGCTACTCTAAAGTTTCA+GGG + chr8_2:44583595-44583614 Msa1249630:three_prime_UTR 30.0%
CTGAAGCATGACTAATATTT+AGG + chr8_2:44583677-44583696 Msa1249630:three_prime_UTR 30.0%
TCTACTAGTATCAAACAATG+TGG + chr8_2:44583767-44583786 Msa1249630:three_prime_UTR 30.0%
CTACTAGTATCAAACAATGT+GGG + chr8_2:44583768-44583787 Msa1249630:three_prime_UTR 30.0%
TATACACAACCTAAAATAGG+AGG - chr8_2:44583794-44583813 None:intergenic 30.0%
! TCAAAACACTAATGTTGGTT+TGG + chr8_2:44583841-44583860 Msa1249630:three_prime_UTR 30.0%
! GTGTTATTGAAGAATAGGGA+GGG - chr8_2:44580219-44580238 None:intergenic 35.0%
! TGTGTTATTGAAGAATAGGG+AGG - chr8_2:44580220-44580239 None:intergenic 35.0%
! GGGTGTGTTATTGAAGAATA+GGG - chr8_2:44580223-44580242 None:intergenic 35.0%
! TGGGTGTGTTATTGAAGAAT+AGG - chr8_2:44580224-44580243 None:intergenic 35.0%
ATATTACCAATGATCCTGGT+GGG - chr8_2:44580297-44580316 None:intergenic 35.0%
TATATTACCAATGATCCTGG+TGG - chr8_2:44580298-44580317 None:intergenic 35.0%
ACTGATAAACACCTTGACTT+AGG + chr8_2:44580707-44580726 Msa1249630:intron 35.0%
TAGCTAAATAATGAGCAGCT+TGG + chr8_2:44580921-44580940 Msa1249630:intron 35.0%
TAAATAATGAGCAGCTTGGT+TGG + chr8_2:44580925-44580944 Msa1249630:intron 35.0%
CCTAACATGCAAAACTGTTT+GGG + chr8_2:44580955-44580974 Msa1249630:intron 35.0%
!!! AACCACAAGTCAGTGTTTTT+TGG - chr8_2:44581225-44581244 None:intergenic 35.0%
! ATACTTGTTAGCTAAGGCAA+TGG - chr8_2:44581292-44581311 None:intergenic 35.0%
ATTGCCTTAGCTAACAAGTA+TGG + chr8_2:44581291-44581310 Msa1249630:CDS 35.0%
AACCTTCAGATAAATGCCAA+CGG - chr8_2:44581525-44581544 None:intergenic 35.0%
GTTGATATTACTCGCTTTAG+AGG + chr8_2:44581730-44581749 Msa1249630:intron 35.0%
!!! ACTGTGTCCTTTTTTTGTTG+AGG + chr8_2:44581754-44581773 Msa1249630:intron 35.0%
TCAAGGACCTCAACAAAAAA+AGG - chr8_2:44581764-44581783 None:intergenic 35.0%
!!! TTTTTTGTTGAGGTCCTTGA+AGG + chr8_2:44581764-44581783 Msa1249630:intron 35.0%
!! GTTCAACTACTATCGATTTG+TGG + chr8_2:44581865-44581884 Msa1249630:intron 35.0%
TTAAGAGCCAAGTGTGTTTA+GGG + chr8_2:44581913-44581932 Msa1249630:intron 35.0%
! AGCTAATACTTGGAGCTTTT+GGG + chr8_2:44581958-44581977 Msa1249630:intron 35.0%
ATTACCAAACACGCGATAAA+AGG + chr8_2:44582035-44582054 Msa1249630:intron 35.0%
ACACAGTGAATTATCGGATA+TGG + chr8_2:44582064-44582083 Msa1249630:intron 35.0%
TGGAAAAAAGTGGTCTATCA+TGG - chr8_2:44582124-44582143 None:intergenic 35.0%
CATCCAACCAAACTTTATCA+TGG + chr8_2:44582158-44582177 Msa1249630:intron 35.0%
AGACCATGATAAAGTTTGGT+TGG - chr8_2:44582164-44582183 None:intergenic 35.0%
GGTTAGACCATGATAAAGTT+TGG - chr8_2:44582168-44582187 None:intergenic 35.0%
! CACATGGTATTTCTATGACA+TGG - chr8_2:44582307-44582326 None:intergenic 35.0%
TCATAGAAATACCATGTGCA+AGG + chr8_2:44582309-44582328 Msa1249630:intron 35.0%
ATTACATGCTTGCTTGGTAA+TGG - chr8_2:44582394-44582413 None:intergenic 35.0%
! TTTTGGATTACATGCTTGCT+TGG - chr8_2:44582400-44582419 None:intergenic 35.0%
!!! TGTGGCTTTGGTTTTCATTT+TGG - chr8_2:44582417-44582436 None:intergenic 35.0%
CAGCATCATCAATTTCTACT+AGG + chr8_2:44582442-44582461 Msa1249630:intron 35.0%
AGTTCCTTTCTCTTTCAAAG+TGG - chr8_2:44582567-44582586 None:intergenic 35.0%
AGTTGGACTGTGATAAGATT+TGG + chr8_2:44582639-44582658 Msa1249630:intron 35.0%
! ATCAAGGACCAGTCTTTTTA+AGG + chr8_2:44582698-44582717 Msa1249630:intron 35.0%
! TCAAGGACCAGTCTTTTTAA+GGG + chr8_2:44582699-44582718 Msa1249630:intron 35.0%
AAATGGACCCTTAAAAAGAC+TGG - chr8_2:44582709-44582728 None:intergenic 35.0%
ACACCAGATATCATATCCTA+GGG - chr8_2:44582740-44582759 None:intergenic 35.0%
AACACCAGATATCATATCCT+AGG - chr8_2:44582741-44582760 None:intergenic 35.0%
!! CCATGGCATTATTTTGTCTT+GGG + chr8_2:44582830-44582849 Msa1249630:intron 35.0%
TAATAAAATTCATCCCCGAG+TGG - chr8_2:44582923-44582942 None:intergenic 35.0%
! ACTCGGGGATGAATTTTATT+AGG + chr8_2:44582922-44582941 Msa1249630:intron 35.0%
AGTAAGCCAACAGCAATAAT+TGG + chr8_2:44583375-44583394 Msa1249630:CDS 35.0%
! GGATTTGGTAGTTATTAGCA+TGG + chr8_2:44583422-44583441 Msa1249630:CDS 35.0%
CTACAAAATGCGAACTACAA+TGG - chr8_2:44583519-44583538 None:intergenic 35.0%
! TAGTTCGCATTTTGTAGACA+GGG + chr8_2:44583522-44583541 Msa1249630:exon 35.0%
ACTAATATTTAGGCCTGGAA+AGG + chr8_2:44583687-44583706 Msa1249630:three_prime_UTR 35.0%
AAACTCATCATTTCCTTTCC+AGG - chr8_2:44583703-44583722 None:intergenic 35.0%
AAGGAAATGATGAGTTTGTG+AGG + chr8_2:44583706-44583725 Msa1249630:three_prime_UTR 35.0%
GATTTGGATTCTTTCATGTG+AGG + chr8_2:44583733-44583752 Msa1249630:three_prime_UTR 35.0%
CAAGATTCTAGTGACTTCCA+GGG - chr8_2:44580092-44580111 None:intergenic 40.0%
ACAAGATTCTAGTGACTTCC+AGG - chr8_2:44580093-44580112 None:intergenic 40.0%
!!! GGTTTTGGAGAGATAAGAGA+GGG - chr8_2:44580198-44580217 None:intergenic 40.0%
! TTGAAGAATAGGGAGGGTTT+TGG - chr8_2:44580213-44580232 None:intergenic 40.0%
TATTACCAATGATCCTGGTG+GGG - chr8_2:44580296-44580315 None:intergenic 40.0%
TAGAAAACCCTATCTCTCTG+AGG + chr8_2:44580590-44580609 Msa1249630:CDS 40.0%
AGAAAACCCTATCTCTCTGA+GGG + chr8_2:44580591-44580610 Msa1249630:CDS 40.0%
TTAGTTCCCTCAGAGAGATA+GGG - chr8_2:44580600-44580619 None:intergenic 40.0%
! TTTAGTTCCCTCAGAGAGAT+AGG - chr8_2:44580601-44580620 None:intergenic 40.0%
! ACCTTGACTTAGGACTTGTT+TGG + chr8_2:44580717-44580736 Msa1249630:intron 40.0%
TCCAAACAAGTCCTAAGTCA+AGG - chr8_2:44580721-44580740 None:intergenic 40.0%
TAAGCACTTGTGAGACTGTT+TGG + chr8_2:44580756-44580775 Msa1249630:intron 40.0%
AAGCACTTGTGAGACTGTTT+GGG + chr8_2:44580757-44580776 Msa1249630:intron 40.0%
AAGACTGCAGAAATACAAGG+CGG + chr8_2:44580854-44580873 Msa1249630:intron 40.0%
GCCTAACATGCAAAACTGTT+TGG + chr8_2:44580954-44580973 Msa1249630:intron 40.0%
! CCCAAACAGTTTTGCATGTT+AGG - chr8_2:44580958-44580977 None:intergenic 40.0%
CACAAAATTAACGCCCTGTT+TGG - chr8_2:44581192-44581211 None:intergenic 40.0%
!! CACCAAAAAACACTGACTTG+TGG + chr8_2:44581220-44581239 Msa1249630:intron 40.0%
!! AAAACACTGACTTGTGGTTG+TGG + chr8_2:44581226-44581245 Msa1249630:intron 40.0%
! AGCTCCATACTTGTTAGCTA+AGG - chr8_2:44581298-44581317 None:intergenic 40.0%
AGTTATCGATGAACAGCAGA+AGG + chr8_2:44581462-44581481 Msa1249630:intron 40.0%
ATCCGTTGGCATTTATCTGA+AGG + chr8_2:44581520-44581539 Msa1249630:CDS 40.0%
CTTAAGAGCCAAGTGTGTTT+AGG + chr8_2:44581912-44581931 Msa1249630:intron 40.0%
TAAGAGCCAAGTGTGTTTAG+GGG + chr8_2:44581914-44581933 Msa1249630:intron 40.0%
ATTAGCTCCAACCTACTTTG+CGG - chr8_2:44581945-44581964 None:intergenic 40.0%
! AGTAGGTTGGAGCTAATACT+TGG + chr8_2:44581948-44581967 Msa1249630:intron 40.0%
! GAGCTAATACTTGGAGCTTT+TGG + chr8_2:44581957-44581976 Msa1249630:intron 40.0%
!! TTGGAGCTTTTGGGAAATTG+CGG + chr8_2:44581967-44581986 Msa1249630:intron 40.0%
GAAGCTTAAACATGAGGTAG+AGG + chr8_2:44581996-44582015 Msa1249630:intron 40.0%
! ACATCCTTTTATCGCGTGTT+TGG - chr8_2:44582042-44582061 None:intergenic 40.0%
! ATCGGATATGGAGTCCTTTT+AGG + chr8_2:44582076-44582095 Msa1249630:intron 40.0%
!! GATATGGAGTCCTTTTAGGT+TGG + chr8_2:44582080-44582099 Msa1249630:intron 40.0%
TTCAACGGATCCAACCTAAA+AGG - chr8_2:44582093-44582112 None:intergenic 40.0%
ATGAGAGAGATGGAGTCAAT+GGG - chr8_2:44582204-44582223 None:intergenic 40.0%
AATGAGAGAGATGGAGTCAA+TGG - chr8_2:44582205-44582224 None:intergenic 40.0%
AGCATAGCAGTAAAGGGATT+GGG - chr8_2:44582282-44582301 None:intergenic 40.0%
! ATGGAGAGCATAGCAGTAAA+GGG - chr8_2:44582288-44582307 None:intergenic 40.0%
TGAGCAAATCCAAAACCGAT+AGG + chr8_2:44582361-44582380 Msa1249630:intron 40.0%
!! ATTGATGATGCTGTCAACTG+TGG - chr8_2:44582435-44582454 None:intergenic 40.0%
AGGATTGTGGCATCATTTGA+AGG + chr8_2:44582462-44582481 Msa1249630:intron 40.0%
! TCAGCCACTTTGAAAGAGAA+AGG + chr8_2:44582560-44582579 Msa1249630:intron 40.0%
TGGACTGTGATAAGATTTGG+TGG + chr8_2:44582642-44582661 Msa1249630:intron 40.0%
! GGACTGTGATAAGATTTGGT+GGG + chr8_2:44582643-44582662 Msa1249630:intron 40.0%
ATCCTAGGGCCCATAAAAAA+TGG - chr8_2:44582726-44582745 None:intergenic 40.0%
CCATGGCTCAATTACTTGTA+TGG - chr8_2:44582816-44582835 None:intergenic 40.0%
CCATACAAGTAATTGAGCCA+TGG + chr8_2:44582813-44582832 Msa1249630:intron 40.0%
!! GCCATGGCATTATTTTGTCT+TGG + chr8_2:44582829-44582848 Msa1249630:intron 40.0%
CCCAAGACAAAATAATGCCA+TGG - chr8_2:44582833-44582852 None:intergenic 40.0%
TCAAATGCACTGTCTGTGTT+AGG + chr8_2:44583125-44583144 Msa1249630:intron 40.0%
GGTATATAAGTGTGCGCTTA+AGG + chr8_2:44583241-44583260 Msa1249630:intron 40.0%
AGGACTACAAATTGCTTGAG+CGG + chr8_2:44583340-44583359 Msa1249630:CDS 40.0%
ACATCGCCAATTATTGCTGT+TGG - chr8_2:44583384-44583403 None:intergenic 40.0%
! TACAATGGCAAAAGCATGAC+AGG - chr8_2:44583504-44583523 None:intergenic 40.0%
! GTAGTTCGCATTTTGTAGAC+AGG + chr8_2:44583521-44583540 Msa1249630:exon 40.0%
TGCATGACTGTACTTGACTA+AGG + chr8_2:44583633-44583652 Msa1249630:three_prime_UTR 40.0%
GCATGACTAATATTTAGGCC+TGG + chr8_2:44583682-44583701 Msa1249630:three_prime_UTR 40.0%
AATGATGAGTTTGTGAGGAG+TGG + chr8_2:44583711-44583730 Msa1249630:three_prime_UTR 40.0%
CGGTTACTTCTCTGAGTTTG+GGG - chr8_2:44580127-44580146 None:intergenic 45.0%
GCGGTTACTTCTCTGAGTTT+GGG - chr8_2:44580128-44580147 None:intergenic 45.0%
TGCGGTTACTTCTCTGAGTT+TGG - chr8_2:44580129-44580148 None:intergenic 45.0%
!!! GGGTTTTGGAGAGATAAGAG+AGG - chr8_2:44580199-44580218 None:intergenic 45.0%
!! AAGTGGGAGGTGATGGAAAT+GGG - chr8_2:44580243-44580262 None:intergenic 45.0%
!! AAAGTGGGAGGTGATGGAAA+TGG - chr8_2:44580244-44580263 None:intergenic 45.0%
GTTGAAAAAGTGGGAGGTGA+TGG - chr8_2:44580250-44580269 None:intergenic 45.0%
! ACAGCAACTTCAGGTTCTTG+TGG - chr8_2:44580519-44580538 None:intergenic 45.0%
TCAGTAGCTACAGCAACTTC+AGG - chr8_2:44580528-44580547 None:intergenic 45.0%
! TCTCTCTGAGGGAACTAAAC+AGG + chr8_2:44580602-44580621 Msa1249630:CDS 45.0%
GCCAAGACTGCAGAAATACA+AGG + chr8_2:44580851-44580870 Msa1249630:intron 45.0%
! GCCTTGTATTTCTGCAGTCT+TGG - chr8_2:44580855-44580874 None:intergenic 45.0%
GGAGCTGACATTACAGTTGT+AGG + chr8_2:44581312-44581331 Msa1249630:CDS 45.0%
AGATAAATGCCAACGGATGC+TGG - chr8_2:44581518-44581537 None:intergenic 45.0%
!! AGCTTTTGGGAAATTGCGGT+GGG + chr8_2:44581971-44581990 Msa1249630:intron 45.0%
TGGGAGGAAGCTTAAACATG+AGG + chr8_2:44581990-44582009 Msa1249630:intron 45.0%
!! TCAATGGGTTAGATCTGGTG+TGG - chr8_2:44582189-44582208 None:intergenic 45.0%
!! TGGAGTCAATGGGTTAGATC+TGG - chr8_2:44582194-44582213 None:intergenic 45.0%
GAGCATAGCAGTAAAGGGAT+TGG - chr8_2:44582283-44582302 None:intergenic 45.0%
! CATGGAGAGCATAGCAGTAA+AGG - chr8_2:44582289-44582308 None:intergenic 45.0%
! GGTAATGGTAGGCTTCCTAT+CGG - chr8_2:44582379-44582398 None:intergenic 45.0%
!! CATGCTTGCTTGGTAATGGT+AGG - chr8_2:44582390-44582409 None:intergenic 45.0%
!!! GTCCATTTTTTATGGGCCCT+AGG + chr8_2:44582721-44582740 Msa1249630:intron 45.0%
GAGTGAGTGAAGAGCAAAAG+AGG + chr8_2:44582867-44582886 Msa1249630:intron 45.0%
TGAAGAGCAAAAGAGGACGA+AGG + chr8_2:44582874-44582893 Msa1249630:intron 45.0%
!! GAAAAATGCAGGTGGGTTGA+AGG + chr8_2:44583320-44583339 Msa1249630:intron 45.0%
TACAAATTGCTTGAGCGGCT+TGG + chr8_2:44583345-44583364 Msa1249630:CDS 45.0%
! GAGTTTGTGAGGAGTGGATT+TGG + chr8_2:44583717-44583736 Msa1249630:three_prime_UTR 45.0%
! CAATGTGGGCCTCCTATTTT+AGG + chr8_2:44583782-44583801 Msa1249630:three_prime_UTR 45.0%
!!! ATTTATTTTTTCTTTTTAAA+AGG + chr8_2:44582960-44582979 Msa1249630:intron 5.0%
GAAGTAACCGCAAGCATCCA+TGG + chr8_2:44580137-44580156 Msa1249630:exon 50.0%
ATGAACGCCATGGATGCTTG+CGG - chr8_2:44580147-44580166 None:intergenic 50.0%
! GAGGGTTTGACGAGTTGAAG+CGG - chr8_2:44580180-44580199 None:intergenic 50.0%
GGAAGGGGGGTTGAAAAAGT+GGG - chr8_2:44580259-44580278 None:intergenic 50.0%
GCTATCTTGGACAGTCTCAC+AGG - chr8_2:44581044-44581063 None:intergenic 50.0%
GATAGTTGGGTCTCATGCTC+AGG - chr8_2:44581496-44581515 None:intergenic 50.0%
CCAACGGATGCTGGATAGTT+GGG - chr8_2:44581509-44581528 None:intergenic 50.0%
GTCACACCCCTAAACACACT+TGG - chr8_2:44581923-44581942 None:intergenic 50.0%
!! GAGCTTTTGGGAAATTGCGG+TGG + chr8_2:44581970-44581989 Msa1249630:intron 50.0%
!! TTTTGGGAAATTGCGGTGGG+AGG + chr8_2:44581974-44581993 Msa1249630:intron 50.0%
! CGCGTGTTTGGTAATCCACT+GGG - chr8_2:44582030-44582049 None:intergenic 50.0%
! TCGCGTGTTTGGTAATCCAC+TGG - chr8_2:44582031-44582050 None:intergenic 50.0%
GAGAGAGGCAATGAGAGAGA+TGG - chr8_2:44582214-44582233 None:intergenic 50.0%
ACTTGCTTCAGCCTTGCACA+TGG - chr8_2:44582323-44582342 None:intergenic 50.0%
!!! GGTAGGCTTCCTATCGGTTT+TGG - chr8_2:44582373-44582392 None:intergenic 50.0%
GATGCTGTCAACTGTGGCTT+TGG - chr8_2:44582429-44582448 None:intergenic 50.0%
GGGCCCTAGGATATGATATC+TGG + chr8_2:44582734-44582753 Msa1249630:intron 50.0%
AGAGCAAAAGAGGACGAAGG+AGG + chr8_2:44582877-44582896 Msa1249630:intron 50.0%
CAAAAGAGGACGAAGGAGGA+AGG + chr8_2:44582881-44582900 Msa1249630:intron 50.0%
AGGAAGGACGAGGGAGATAA+GGG + chr8_2:44582897-44582916 Msa1249630:intron 50.0%
! GGCAAAAGCATGACAGGACA+GGG - chr8_2:44583498-44583517 None:intergenic 50.0%
! TGGCAAAAGCATGACAGGAC+AGG - chr8_2:44583499-44583518 None:intergenic 50.0%
TGGCAGAGTCATGAACGCCA+TGG - chr8_2:44580157-44580176 None:intergenic 55.0%
! GGTTTGACGAGTTGAAGCGG+TGG - chr8_2:44580177-44580196 None:intergenic 55.0%
AGGGGGGTTGAAAAAGTGGG+AGG - chr8_2:44580256-44580275 None:intergenic 55.0%
GGGAAGGGGGGTTGAAAAAG+TGG - chr8_2:44580260-44580279 None:intergenic 55.0%
ACCAATGATCCTGGTGGGGA+GGG - chr8_2:44580292-44580311 None:intergenic 55.0%
TACCAATGATCCTGGTGGGG+AGG - chr8_2:44580293-44580312 None:intergenic 55.0%
CCCAACTATCCAGCATCCGT+TGG + chr8_2:44581506-44581525 Msa1249630:CDS 55.0%
GCCAACGGATGCTGGATAGT+TGG - chr8_2:44581510-44581529 None:intergenic 55.0%
! TAGGGGTGTGACCGCAAAGT+AGG + chr8_2:44581931-44581950 Msa1249630:intron 55.0%
GGTGTGACCGCAAAGTAGGT+TGG + chr8_2:44581935-44581954 Msa1249630:intron 55.0%
GTAGAGGTGATGTGACCCAG+TGG + chr8_2:44582012-44582031 Msa1249630:intron 55.0%
GCAGTAAAGGGATTGGGAGC+AGG - chr8_2:44582276-44582295 None:intergenic 55.0%
GAGGAAGGACGAGGGAGATA+AGG + chr8_2:44582896-44582915 Msa1249630:intron 55.0%
!! TTGCTTGAGCGGCTTGGTGA+TGG + chr8_2:44583351-44583370 Msa1249630:CDS 55.0%
!! TGCTTGAGCGGCTTGGTGAT+GGG + chr8_2:44583352-44583371 Msa1249630:CDS 55.0%
AGGGTGTGAAGTCGGGAAGG+GGG - chr8_2:44580273-44580292 None:intergenic 60.0%
! GAGGGTGTGAAGTCGGGAAG+GGG - chr8_2:44580274-44580293 None:intergenic 60.0%
! GGAGGGTGTGAAGTCGGGAA+GGG - chr8_2:44580275-44580294 None:intergenic 60.0%
! TGGTGGGGAGGGTGTGAAGT+CGG - chr8_2:44580281-44580300 None:intergenic 60.0%
ACCCTCCCCACCAGGATCAT+TGG + chr8_2:44580288-44580307 Msa1249630:CDS 60.0%
AGGACGAAGGAGGAAGGACG+AGG + chr8_2:44582887-44582906 Msa1249630:intron 60.0%
GGACGAAGGAGGAAGGACGA+GGG + chr8_2:44582888-44582907 Msa1249630:intron 60.0%
CGAGGGAGATAAGGGCCACT+CGG + chr8_2:44582905-44582924 Msa1249630:intron 60.0%
GAGGGAGATAAGGGCCACTC+GGG + chr8_2:44582906-44582925 Msa1249630:intron 60.0%
AGGGAGATAAGGGCCACTCG+GGG + chr8_2:44582907-44582926 Msa1249630:intron 60.0%
GGGTGTGAAGTCGGGAAGGG+GGG - chr8_2:44580272-44580291 None:intergenic 65.0%
! GGGAGGGTGTGAAGTCGGGA+AGG - chr8_2:44580276-44580295 None:intergenic 65.0%
! GGTGGGGAGGGTGTGAAGTC+GGG - chr8_2:44580280-44580299 None:intergenic 65.0%
GACTTCACACCCTCCCCACC+AGG + chr8_2:44580280-44580299 Msa1249630:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 44579973 44583960 44579973 ID=Msa1249630;Name=Msa1249630
chr8_2 mRNA 44579973 44583960 44579973 ID=Msa1249630-mRNA-1;Parent=Msa1249630;Name=Msa1249630-mRNA-1;_AED=0.17;_eAED=0.17;_QI=183|1|1|1|1|1|5|436|200
chr8_2 exon 44579973 44580309 44579973 ID=Msa1249630-mRNA-1:exon:13492;Parent=Msa1249630-mRNA-1
chr8_2 exon 44580505 44580623 44580505 ID=Msa1249630-mRNA-1:exon:13493;Parent=Msa1249630-mRNA-1
chr8_2 exon 44581276 44581333 44581276 ID=Msa1249630-mRNA-1:exon:13494;Parent=Msa1249630-mRNA-1
chr8_2 exon 44581464 44581541 44581464 ID=Msa1249630-mRNA-1:exon:13495;Parent=Msa1249630-mRNA-1
chr8_2 exon 44583331 44583960 44583331 ID=Msa1249630-mRNA-1:exon:13496;Parent=Msa1249630-mRNA-1
chr8_2 five_prime_UTR 44579973 44580155 44579973 ID=Msa1249630-mRNA-1:five_prime_utr;Parent=Msa1249630-mRNA-1
chr8_2 CDS 44580156 44580309 44580156 ID=Msa1249630-mRNA-1:cds;Parent=Msa1249630-mRNA-1
chr8_2 CDS 44580505 44580623 44580505 ID=Msa1249630-mRNA-1:cds;Parent=Msa1249630-mRNA-1
chr8_2 CDS 44581276 44581333 44581276 ID=Msa1249630-mRNA-1:cds;Parent=Msa1249630-mRNA-1
chr8_2 CDS 44581464 44581541 44581464 ID=Msa1249630-mRNA-1:cds;Parent=Msa1249630-mRNA-1
chr8_2 CDS 44583331 44583524 44583331 ID=Msa1249630-mRNA-1:cds;Parent=Msa1249630-mRNA-1
chr8_2 three_prime_UTR 44583525 44583960 44583525 ID=Msa1249630-mRNA-1:three_prime_utr;Parent=Msa1249630-mRNA-1
Gene Sequence

>Msa1249630

ATGGCGTTCATGACTCTGCCACCGCTTCAACTCGTCAAACCCTCTCTTATCTCTCCAAAACCCTCCCTATTCTTCAATAACACACCCATTTCCATCACCTCCCACTTTTTCAACCCCCCTTCCCGACTTCACACCCTCCCCACCAGGATCATTGTTAAGGCTAAACCACAAGAACCTGAAGTTGCTGTAGCTACTGATTCATTTACACAATTCAATCATCTTCTTTTGCCTATTACTGATAGAAAACCCTATCTCTCTGAGGGAACTAAACAGGCTATAGCGACTACCATTGCCTTAGCTAACAAGTATGGAGCTGACATTACAGTTGTAGTTATCGATGAACAGCAGAAGGAGTCACTGCCTGAGCATGAGACCCAACTATCCAGCATCCGTTGGCATTTATCTGAAGGTGGGTTGAAGGACTACAAATTGCTTGAGCGGCTTGGTGATGGGAGTAAGCCAACAGCAATAATTGGCGATGTTGCTGATGAACTTAATTTGGATTTGGTAGTTATTAGCATGGAAGCAATTCATACAAAGCATATAGATGCAAATTTGCTTGCTGAGTTCATTCCCTGTCCTGTCATGCTTTTGCCATTGTAG
Protein sequence

>Msa1249630

MAFMTLPPLQLVKPSLISPKPSLFFNNTPISITSHFFNPPSRLHTLPTRIIVKAKPQEPEVAVATDSFTQFNHLLLPITDRKPYLSEGTKQAIATTIALANKYGADITVVVIDEQQKESLPEHETQLSSIRWHLSEGGLKDYKLLERLGDGSKPTAIIGDVADELNLDLVVISMEAIHTKHIDANLLAEFIPCPVMLLPL*