AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1294600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294600 MtrunA17_Chr8g0371391 95.522 201 8 1 1 200 1 201 1.70e-137 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294600 AT5G66090.1 66.258 163 49 2 43 200 49 210 2.02e-75 226

Find 84 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATATATTGTAATCAATTT+TGG 0.136663 8_3:+52404262 Msa1294600:three_prime_UTR
TGTTGCTGATGAACTTAATT+TGG 0.180753 8_3:+52403734 Msa1294600:CDS
ATATGCTACTCTAAAGTTTC+AGG 0.183780 8_3:+52403927 Msa1294600:three_prime_UTR
TGCGGTTACTTCTCTGAGTT+TGG 0.226890 8_3:-52400467 None:intergenic
TTGAAGAATAGGGAGGGTTT+TGG 0.277998 8_3:-52400551 None:intergenic
AAACTCATCATTTCCTTTCC+AGG 0.296477 8_3:-52404033 None:intergenic
CTGAAGCATGACTAATATTT+AGG 0.306734 8_3:+52404010 Msa1294600:three_prime_UTR
TTTAGTTCCCTCAGAGAGAT+AGG 0.318311 8_3:-52400939 None:intergenic
GAGTTTGTGAGGAGTGGATT+TGG 0.319040 8_3:+52404050 Msa1294600:three_prime_UTR
GCGGTTACTTCTCTGAGTTT+GGG 0.329354 8_3:-52400466 None:intergenic
GATTATACACAACCTAAAAT+AGG 0.332466 8_3:-52404127 None:intergenic
GAGGGTGTGAAGTCGGGAAG+GGG 0.335959 8_3:-52400612 None:intergenic
AGGGTGTGAAGTCGGGAAGG+GGG 0.337342 8_3:-52400611 None:intergenic
AGTAAGCCAACAGCAATAAT+TGG 0.349886 8_3:+52403708 Msa1294600:CDS
TCAAAACACTAATGTTGGTT+TGG 0.369198 8_3:+52404174 Msa1294600:three_prime_UTR
TTTATCAATTTCGTTGTTTG+AGG 0.383976 8_3:-52400400 None:intergenic
GGGTGTGAAGTCGGGAAGGG+GGG 0.386173 8_3:-52400610 None:intergenic
TGGGTGTGTTATTGAAGAAT+AGG 0.393378 8_3:-52400562 None:intergenic
GGTGGGGAGGGTGTGAAGTC+GGG 0.401871 8_3:-52400618 None:intergenic
TGATGAACTTAATTTGGATT+TGG 0.404360 8_3:+52403740 Msa1294600:CDS
AGATAAATGCCAACGGATGC+TGG 0.406698 8_3:-52401847 None:intergenic
ATTGCCTTAGCTAACAAGTA+TGG 0.418236 8_3:+52401623 Msa1294600:CDS
ACCCTCCCCACCAGGATCAT+TGG 0.426256 8_3:+52400629 Msa1294600:CDS
TCTCTCTGAGGGAACTAAAC+AGG 0.436554 8_3:+52400943 Msa1294600:CDS
GGGTGTGTTATTGAAGAATA+GGG 0.438335 8_3:-52400561 None:intergenic
TCAGTAGCTACAGCAACTTC+AGG 0.444631 8_3:-52400866 None:intergenic
AAGTGGGAGGTGATGGAAAT+GGG 0.445737 8_3:-52400581 None:intergenic
TACAAATTGCTTGAGCGGCT+TGG 0.450205 8_3:+52403678 Msa1294600:CDS
GCCAACGGATGCTGGATAGT+TGG 0.451723 8_3:-52401839 None:intergenic
CCAACGGATGCTGGATAGTT+GGG 0.456468 8_3:-52401838 None:intergenic
GATAGTTGGGTCTCATGCTC+AGG 0.456507 8_3:-52401825 None:intergenic
GGGAGGGTGTGAAGTCGGGA+AGG 0.458649 8_3:-52400614 None:intergenic
GGAGGGTGTGAAGTCGGGAA+GGG 0.463065 8_3:-52400613 None:intergenic
TTGCTTGAGCGGCTTGGTGA+TGG 0.467825 8_3:+52403684 Msa1294600:CDS
ATTATTCAAAACACTAATGT+TGG 0.468386 8_3:+52404169 Msa1294600:three_prime_UTR
AAAGTGGGAGGTGATGGAAA+TGG 0.468946 8_3:-52400582 None:intergenic
GGAGCTGACATTACAGTTGT+AGG 0.469978 8_3:+52401644 Msa1294600:CDS
TGGCAAAAGCATGACAGGAC+AGG 0.472545 8_3:-52403829 None:intergenic
ACATCGCCAATTATTGCTGT+TGG 0.484894 8_3:-52403714 None:intergenic
ACCAATGATCCTGGTGGGGA+GGG 0.491868 8_3:-52400630 None:intergenic
ATCCGTTGGCATTTATCTGA+AGG 0.506635 8_3:+52401852 Msa1294600:CDS
TATGCTACTCTAAAGTTTCA+GGG 0.507896 8_3:+52403928 Msa1294600:three_prime_UTR
TGGTGGGGAGGGTGTGAAGT+CGG 0.513130 8_3:-52400619 None:intergenic
GACTTCACACCCTCCCCACC+AGG 0.519206 8_3:+52400621 Msa1294600:CDS
TACAATGGCAAAAGCATGAC+AGG 0.519854 8_3:-52403834 None:intergenic
TTAGTTCCCTCAGAGAGATA+GGG 0.523045 8_3:-52400938 None:intergenic
TGCTTGAGCGGCTTGGTGAT+GGG 0.524122 8_3:+52403685 Msa1294600:CDS
AATGATGAGTTTGTGAGGAG+TGG 0.527912 8_3:+52404044 Msa1294600:three_prime_UTR
AGTTATATTACCAATGATCC+TGG 0.538740 8_3:-52400639 None:intergenic
GAAGTAACCGCAAGCATCCA+TGG 0.542093 8_3:+52400478 Msa1294600:exon
AGCTCCATACTTGTTAGCTA+AGG 0.544707 8_3:-52401627 None:intergenic
TGCATGACTGTACTTGACTA+AGG 0.547431 8_3:+52403966 Msa1294600:three_prime_UTR
GTGTTATTGAAGAATAGGGA+GGG 0.550609 8_3:-52400557 None:intergenic
ACAGCAACTTCAGGTTCTTG+TGG 0.551790 8_3:-52400857 None:intergenic
ACTAATATTTAGGCCTGGAA+AGG 0.553070 8_3:+52404020 Msa1294600:three_prime_UTR
GCATGACTAATATTTAGGCC+TGG 0.555476 8_3:+52404015 Msa1294600:three_prime_UTR
CGGTTACTTCTCTGAGTTTG+GGG 0.556267 8_3:-52400465 None:intergenic
ATATTACCAATGATCCTGGT+GGG 0.557905 8_3:-52400635 None:intergenic
CTACTAGTATCAAACAATGT+GGG 0.560265 8_3:+52404101 Msa1294600:three_prime_UTR
TACCAATGATCCTGGTGGGG+AGG 0.564673 8_3:-52400631 None:intergenic
GGATTTGGTAGTTATTAGCA+TGG 0.566741 8_3:+52403755 Msa1294600:CDS
CAAGATTCTAGTGACTTCCA+CGG 0.575836 8_3:-52400430 None:intergenic
AAGATTCTAGTGACTTCCAC+GGG 0.576868 8_3:-52400429 None:intergenic
AGGACTACAAATTGCTTGAG+CGG 0.586994 8_3:+52403673 Msa1294600:CDS
CTACAAAATGCGAACTACAA+TGG 0.587815 8_3:-52403849 None:intergenic
CCCAACTATCCAGCATCCGT+TGG 0.587965 8_3:+52401838 Msa1294600:CDS
GGCAAAAGCATGACAGGACA+GGG 0.588085 8_3:-52403828 None:intergenic
AGAAAACCCTATCTCTCTGA+GGG 0.601139 8_3:+52400932 Msa1294600:CDS
GATTTGGATTCTTTCATGTG+AGG 0.606825 8_3:+52404066 Msa1294600:three_prime_UTR
TATACACAACCTAAAATAGG+AGG 0.607063 8_3:-52404124 None:intergenic
AGTTATCGATGAACAGCAGA+AGG 0.615289 8_3:+52401794 Msa1294600:intron
GAGGGTTTGACGAGTTGAAG+CGG 0.621708 8_3:-52400518 None:intergenic
TCTACTAGTATCAAACAATG+TGG 0.623417 8_3:+52404100 Msa1294600:three_prime_UTR
TATTACCAATGATCCTGGTG+GGG 0.632686 8_3:-52400634 None:intergenic
TGGCAGAGTCATGAACGCCA+TGG 0.640926 8_3:-52400495 None:intergenic
AACCTTCAGATAAATGCCAA+CGG 0.643797 8_3:-52401854 None:intergenic
ATACTTGTTAGCTAAGGCAA+TGG 0.644889 8_3:-52401621 None:intergenic
TGTGTTATTGAAGAATAGGG+AGG 0.650429 8_3:-52400558 None:intergenic
TAGAAAACCCTATCTCTCTG+AGG 0.651248 8_3:+52400931 Msa1294600:CDS
TATATTACCAATGATCCTGG+TGG 0.656745 8_3:-52400636 None:intergenic
ATGAACGCCATGGATGCTTG+CGG 0.669244 8_3:-52400485 None:intergenic
AAGGAAATGATGAGTTTGTG+AGG 0.675607 8_3:+52404039 Msa1294600:three_prime_UTR
AATTGATAAAACGAAACCCG+TGG 0.683798 8_3:+52400413 Msa1294600:five_prime_UTR
GGTTTGACGAGTTGAAGCGG+TGG 0.698692 8_3:-52400515 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATTATCTTTTTGTT+AGG + chr8_3:52401586-52401605 Msa1294600:intron 10.0%
!!! TATGTAAATATTTTTGTTAT+TGG + chr8_3:52402861-52402880 Msa1294600:intron 10.0%
!!! TAGTAATTTTACGTAATTTA+TGG - chr8_3:52400343-52400362 None:intergenic 15.0%
!! AAGAAACTATAATAATTTCT+TGG - chr8_3:52400691-52400710 None:intergenic 15.0%
!!! CTTTTTCTTTTCAATTTATT+TGG + chr8_3:52400971-52400990 Msa1294600:intron 15.0%
!! TAAGAATTTAGATTCTATAT+GGG - chr8_3:52401223-52401242 None:intergenic 15.0%
!! TTAAGAATTTAGATTCTATA+TGG - chr8_3:52401224-52401243 None:intergenic 15.0%
!! AAATTCATAATATCAAAAAG+AGG - chr8_3:52401698-52401717 None:intergenic 15.0%
!!! TGATATTATGAATTTTTTAC+TGG + chr8_3:52401703-52401722 Msa1294600:intron 15.0%
!!! GATATTATGAATTTTTTACT+GGG + chr8_3:52401704-52401723 Msa1294600:intron 15.0%
!!! ATATTATGAATTTTTTACTG+GGG + chr8_3:52401705-52401724 Msa1294600:intron 15.0%
!!! ATTTTTTCTTTTTAAAAGGT+TGG + chr8_3:52403297-52403316 Msa1294600:intron 15.0%
!! TATTTAATAATGAAAAATGC+AGG + chr8_3:52403642-52403661 Msa1294600:intron 15.0%
!!! CTATATATTGTAATCAATTT+TGG + chr8_3:52404262-52404281 Msa1294600:three_prime_UTR 15.0%
!!! TTGATTTTGTAGAAGTTTTT+AGG - chr8_3:52401410-52401429 None:intergenic 20.0%
!!! TTTTTTTGCTTCTCTTTTTT+TGG + chr8_3:52402831-52402850 Msa1294600:intron 20.0%
!!! CCAATGTTTTATTCAAAAAA+CGG - chr8_3:52403395-52403414 None:intergenic 20.0%
!!! CATTTTTAAGTAAACATTCA+TGG + chr8_3:52403553-52403572 Msa1294600:intron 20.0%
!! ATTATTCAAAACACTAATGT+TGG + chr8_3:52404169-52404188 Msa1294600:three_prime_UTR 20.0%
! TTTATCAATTTCGTTGTTTG+AGG - chr8_3:52400403-52400422 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAAAC+AGG + chr8_3:52401507-52401526 Msa1294600:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + chr8_3:52401508-52401527 Msa1294600:intron 25.0%
!!! ATTTGTTGAGTTTTTTTGTG+TGG + chr8_3:52401902-52401921 Msa1294600:intron 25.0%
! AAACTAGTAAAGTGAAAAGT+AGG + chr8_3:52401961-52401980 Msa1294600:intron 25.0%
! TTATTTGTAATGTACCTTCA+AGG - chr8_3:52402113-52402132 None:intergenic 25.0%
!! AATCTAATAGTGGAAAAAAG+TGG - chr8_3:52402467-52402486 None:intergenic 25.0%
! TGGATGATTTAATCTAATAG+TGG - chr8_3:52402477-52402496 None:intergenic 25.0%
! ATTTGCTCATTGTAAAAAAC+AGG - chr8_3:52402683-52402702 None:intergenic 25.0%
!!! TAGAGTTGATGAGTTAAATT+GGG + chr8_3:52402946-52402965 Msa1294600:intron 25.0%
! TAATATGACAGTGTAAAAAG+TGG + chr8_3:52403005-52403024 Msa1294600:intron 25.0%
!!! TTTTAAGGGTCCATTTTTTA+TGG + chr8_3:52403046-52403065 Msa1294600:intron 25.0%
!!! TTTAAGGGTCCATTTTTTAT+GGG + chr8_3:52403047-52403066 Msa1294600:intron 25.0%
!! ATTGAGTAACAGATTTTAAG+TGG + chr8_3:52403328-52403347 Msa1294600:intron 25.0%
! ATAAGACATAGTAAAGGATA+AGG - chr8_3:52403365-52403384 None:intergenic 25.0%
!!! CCGTTTTTTGAATAAAACAT+TGG + chr8_3:52403392-52403411 Msa1294600:intron 25.0%
!!! TTTGATTTGCTACAAAATGA+AGG - chr8_3:52403443-52403462 None:intergenic 25.0%
! TTAATAATGAAAAATGCAGG+TGG + chr8_3:52403645-52403664 Msa1294600:intron 25.0%
! TAATAATGAAAAATGCAGGT+GGG + chr8_3:52403646-52403665 Msa1294600:intron 25.0%
!! TGATGAACTTAATTTGGATT+TGG + chr8_3:52403740-52403759 Msa1294600:CDS 25.0%
! GATTATACACAACCTAAAAT+AGG - chr8_3:52404130-52404149 None:intergenic 25.0%
AGTTATATTACCAATGATCC+TGG - chr8_3:52400642-52400661 None:intergenic 30.0%
TTTCTTTGATGCATGCAATT+TGG + chr8_3:52400705-52400724 Msa1294600:intron 30.0%
TTCTTTGATGCATGCAATTT+GGG + chr8_3:52400706-52400725 Msa1294600:intron 30.0%
GTTTCATCTGAATTCAGTTA+AGG + chr8_3:52400829-52400848 Msa1294600:intron 30.0%
! TAGGGTTTTCTATCAGTAAT+AGG - chr8_3:52400923-52400942 None:intergenic 30.0%
CAATTTCATTAACGAAAGTC+TGG + chr8_3:52401147-52401166 Msa1294600:intron 30.0%
TTATGAACAATGTCATAAGC+TGG - chr8_3:52401326-52401345 None:intergenic 30.0%
! GGTATTTTCATAAGCTATCT+TGG - chr8_3:52401389-52401408 None:intergenic 30.0%
GTTCTTGTATGAAGTTGTAA+TGG + chr8_3:52401727-52401746 Msa1294600:intron 30.0%
!!! TGAGTTTTTTTGTGTGGATT+TGG + chr8_3:52401908-52401927 Msa1294600:intron 30.0%
! CTTTACTAGTTTACTCTCAA+CGG - chr8_3:52401953-52401972 None:intergenic 30.0%
CTCATGATTGCTTATGTATT+TGG + chr8_3:52402006-52402025 Msa1294600:intron 30.0%
ATGATTGCTTATGTATTTGG+AGG + chr8_3:52402009-52402028 Msa1294600:intron 30.0%
!! TCCATTGATTTATTCGTCAA+TGG + chr8_3:52402040-52402059 Msa1294600:intron 30.0%
ACCATTGACGAATAAATCAA+TGG - chr8_3:52402044-52402063 None:intergenic 30.0%
ATGTACACACAGTGAATTAT+CGG + chr8_3:52402391-52402410 Msa1294600:intron 30.0%
ATCATGGATCAATACTTCAA+CGG - chr8_3:52402441-52402460 None:intergenic 30.0%
AGAAAGAAAATATGAGAGAG+AGG - chr8_3:52402562-52402581 None:intergenic 30.0%
ATCAATTTCTACTAGGATTG+TGG + chr8_3:52402782-52402801 Msa1294600:intron 30.0%
!! GTAGAGTTGATGAGTTAAAT+TGG + chr8_3:52402945-52402964 Msa1294600:intron 30.0%
TGAGTTAAATTGGGTTTAGT+TGG + chr8_3:52402955-52402974 Msa1294600:intron 30.0%
GTGTAAAAAGTGGTATATCA+AGG + chr8_3:52403015-52403034 Msa1294600:intron 30.0%
! ATTTTGTCTTGGGTAAGAAA+AGG + chr8_3:52403173-52403192 Msa1294600:intron 30.0%
CATGCAATAAGACATAGTAA+AGG - chr8_3:52403371-52403390 None:intergenic 30.0%
TGTTGCTGATGAACTTAATT+TGG + chr8_3:52403734-52403753 Msa1294600:CDS 30.0%
!! ATATGCTACTCTAAAGTTTC+AGG + chr8_3:52403927-52403946 Msa1294600:three_prime_UTR 30.0%
!! TATGCTACTCTAAAGTTTCA+GGG + chr8_3:52403928-52403947 Msa1294600:three_prime_UTR 30.0%
CTGAAGCATGACTAATATTT+AGG + chr8_3:52404010-52404029 Msa1294600:three_prime_UTR 30.0%
TCTACTAGTATCAAACAATG+TGG + chr8_3:52404100-52404119 Msa1294600:three_prime_UTR 30.0%
CTACTAGTATCAAACAATGT+GGG + chr8_3:52404101-52404120 Msa1294600:three_prime_UTR 30.0%
TATACACAACCTAAAATAGG+AGG - chr8_3:52404127-52404146 None:intergenic 30.0%
! TCAAAACACTAATGTTGGTT+TGG + chr8_3:52404174-52404193 Msa1294600:three_prime_UTR 30.0%
AATTGATAAAACGAAACCCG+TGG + chr8_3:52400413-52400432 Msa1294600:five_prime_UTR 35.0%
! GTGTTATTGAAGAATAGGGA+GGG - chr8_3:52400560-52400579 None:intergenic 35.0%
! TGTGTTATTGAAGAATAGGG+AGG - chr8_3:52400561-52400580 None:intergenic 35.0%
! GGGTGTGTTATTGAAGAATA+GGG - chr8_3:52400564-52400583 None:intergenic 35.0%
! TGGGTGTGTTATTGAAGAAT+AGG - chr8_3:52400565-52400584 None:intergenic 35.0%
ATATTACCAATGATCCTGGT+GGG - chr8_3:52400638-52400657 None:intergenic 35.0%
TATATTACCAATGATCCTGG+TGG - chr8_3:52400639-52400658 None:intergenic 35.0%
ACTGATAAACACCTTGACTT+AGG + chr8_3:52401048-52401067 Msa1294600:intron 35.0%
TAGCTAAATAATGAGCAGCT+TGG + chr8_3:52401253-52401272 Msa1294600:intron 35.0%
TAAATAATGAGCAGCTTGGT+TGG + chr8_3:52401257-52401276 Msa1294600:intron 35.0%
CCTAACATGCAAAACTGTTT+GGG + chr8_3:52401287-52401306 Msa1294600:intron 35.0%
!!! AACCACAAGTCAGTGTTTTT+TGG - chr8_3:52401557-52401576 None:intergenic 35.0%
! ATACTTGTTAGCTAAGGCAA+TGG - chr8_3:52401624-52401643 None:intergenic 35.0%
ATTGCCTTAGCTAACAAGTA+TGG + chr8_3:52401623-52401642 Msa1294600:CDS 35.0%
AACCTTCAGATAAATGCCAA+CGG - chr8_3:52401857-52401876 None:intergenic 35.0%
GTTGATATTACTCGCTTTAG+AGG + chr8_3:52402062-52402081 Msa1294600:intron 35.0%
!!! ACTGTGTCCTTTTTTTGTTG+AGG + chr8_3:52402086-52402105 Msa1294600:intron 35.0%
TCAAGGACCTCAACAAAAAA+AGG - chr8_3:52402096-52402115 None:intergenic 35.0%
!!! TTTTTTGTTGAGGTCCTTGA+AGG + chr8_3:52402096-52402115 Msa1294600:intron 35.0%
!! GTTCAACTACTATCGATTTG+TGG + chr8_3:52402197-52402216 Msa1294600:intron 35.0%
! AGCTAATACTTGGAGCTTTT+GGG + chr8_3:52402291-52402310 Msa1294600:intron 35.0%
ATTACCAAACACGCGATAAA+AGG + chr8_3:52402368-52402387 Msa1294600:intron 35.0%
ACACAGTGAATTATCGGATA+TGG + chr8_3:52402397-52402416 Msa1294600:intron 35.0%
TGGAAAAAAGTGGTCTATCA+TGG - chr8_3:52402457-52402476 None:intergenic 35.0%
CATCCAACCAAACTTTATCA+TGG + chr8_3:52402491-52402510 Msa1294600:intron 35.0%
AGACCATGATAAAGTTTGGT+TGG - chr8_3:52402497-52402516 None:intergenic 35.0%
GGTTAGACCATGATAAAGTT+TGG - chr8_3:52402501-52402520 None:intergenic 35.0%
! CACATGGTATTTCTATGACA+TGG - chr8_3:52402640-52402659 None:intergenic 35.0%
TCATAGAAATACCATGTGCA+AGG + chr8_3:52402642-52402661 Msa1294600:intron 35.0%
TGCTCATTGTAAAAAACAGG+TGG - chr8_3:52402680-52402699 None:intergenic 35.0%
ATTACATGCTTGCTTGGTAA+TGG - chr8_3:52402727-52402746 None:intergenic 35.0%
! TTTTGGATTACATGCTTGCT+TGG - chr8_3:52402733-52402752 None:intergenic 35.0%
!!! TGTGGCTTTGGTTTTCATTT+TGG - chr8_3:52402750-52402769 None:intergenic 35.0%
CAGCATCATCAATTTCTACT+AGG + chr8_3:52402775-52402794 Msa1294600:intron 35.0%
AGTTCCTTTCTCTTTCAAAG+TGG - chr8_3:52402900-52402919 None:intergenic 35.0%
AGTTGGACTGTGATAAGATT+TGG + chr8_3:52402972-52402991 Msa1294600:intron 35.0%
! ATCAAGGACCAGTCTTTTTA+AGG + chr8_3:52403031-52403050 Msa1294600:intron 35.0%
! TCAAGGACCAGTCTTTTTAA+GGG + chr8_3:52403032-52403051 Msa1294600:intron 35.0%
AAATGGACCCTTAAAAAGAC+TGG - chr8_3:52403042-52403061 None:intergenic 35.0%
ACACCAGATATCATATCCTA+GGG - chr8_3:52403073-52403092 None:intergenic 35.0%
AACACCAGATATCATATCCT+AGG - chr8_3:52403074-52403093 None:intergenic 35.0%
!! CCATGGCATTATTTTGTCTT+GGG + chr8_3:52403163-52403182 Msa1294600:intron 35.0%
TAATAAAATTCATCCCCGAG+TGG - chr8_3:52403256-52403275 None:intergenic 35.0%
! ACTCGGGGATGAATTTTATT+AGG + chr8_3:52403255-52403274 Msa1294600:intron 35.0%
AGTAAGCCAACAGCAATAAT+TGG + chr8_3:52403708-52403727 Msa1294600:CDS 35.0%
! GGATTTGGTAGTTATTAGCA+TGG + chr8_3:52403755-52403774 Msa1294600:CDS 35.0%
CTACAAAATGCGAACTACAA+TGG - chr8_3:52403852-52403871 None:intergenic 35.0%
! TAGTTCGCATTTTGTAGACA+GGG + chr8_3:52403855-52403874 Msa1294600:exon 35.0%
ACTAATATTTAGGCCTGGAA+AGG + chr8_3:52404020-52404039 Msa1294600:three_prime_UTR 35.0%
AAACTCATCATTTCCTTTCC+AGG - chr8_3:52404036-52404055 None:intergenic 35.0%
AAGGAAATGATGAGTTTGTG+AGG + chr8_3:52404039-52404058 Msa1294600:three_prime_UTR 35.0%
GATTTGGATTCTTTCATGTG+AGG + chr8_3:52404066-52404085 Msa1294600:three_prime_UTR 35.0%
AAGATTCTAGTGACTTCCAC+GGG - chr8_3:52400432-52400451 None:intergenic 40.0%
CAAGATTCTAGTGACTTCCA+CGG - chr8_3:52400433-52400452 None:intergenic 40.0%
!!! GGTTTTGGAGAGATAAGAGA+GGG - chr8_3:52400539-52400558 None:intergenic 40.0%
! TTGAAGAATAGGGAGGGTTT+TGG - chr8_3:52400554-52400573 None:intergenic 40.0%
TATTACCAATGATCCTGGTG+GGG - chr8_3:52400637-52400656 None:intergenic 40.0%
TAGAAAACCCTATCTCTCTG+AGG + chr8_3:52400931-52400950 Msa1294600:CDS 40.0%
AGAAAACCCTATCTCTCTGA+GGG + chr8_3:52400932-52400951 Msa1294600:CDS 40.0%
TTAGTTCCCTCAGAGAGATA+GGG - chr8_3:52400941-52400960 None:intergenic 40.0%
! TTTAGTTCCCTCAGAGAGAT+AGG - chr8_3:52400942-52400961 None:intergenic 40.0%
! ACCTTGACTTAGGACTTGTT+TGG + chr8_3:52401058-52401077 Msa1294600:intron 40.0%
TCCAAACAAGTCCTAAGTCA+AGG - chr8_3:52401062-52401081 None:intergenic 40.0%
TAAGCACTTGTGAGACTGTT+TGG + chr8_3:52401097-52401116 Msa1294600:intron 40.0%
AAGCACTTGTGAGACTGTTT+GGG + chr8_3:52401098-52401117 Msa1294600:intron 40.0%
AAGACTGCAGAAATACAAGG+CGG + chr8_3:52401186-52401205 Msa1294600:intron 40.0%
GCCTAACATGCAAAACTGTT+TGG + chr8_3:52401286-52401305 Msa1294600:intron 40.0%
! CCCAAACAGTTTTGCATGTT+AGG - chr8_3:52401290-52401309 None:intergenic 40.0%
CACAAAATTAACGCCCTGTT+TGG - chr8_3:52401524-52401543 None:intergenic 40.0%
!! CACCAAAAAACACTGACTTG+TGG + chr8_3:52401552-52401571 Msa1294600:intron 40.0%
!! AAAACACTGACTTGTGGTTG+TGG + chr8_3:52401558-52401577 Msa1294600:intron 40.0%
! AGCTCCATACTTGTTAGCTA+AGG - chr8_3:52401630-52401649 None:intergenic 40.0%
AGTTATCGATGAACAGCAGA+AGG + chr8_3:52401794-52401813 Msa1294600:intron 40.0%
ATCCGTTGGCATTTATCTGA+AGG + chr8_3:52401852-52401871 Msa1294600:CDS 40.0%
TTAAGAGCCAAGTGTGTGTT+AGG + chr8_3:52402245-52402264 Msa1294600:intron 40.0%
TAAGAGCCAAGTGTGTGTTA+GGG + chr8_3:52402246-52402265 Msa1294600:intron 40.0%
ATTAGCTCCAACCTACTTTG+CGG - chr8_3:52402278-52402297 None:intergenic 40.0%
! AGTAGGTTGGAGCTAATACT+TGG + chr8_3:52402281-52402300 Msa1294600:intron 40.0%
! GAGCTAATACTTGGAGCTTT+TGG + chr8_3:52402290-52402309 Msa1294600:intron 40.0%
!! TTGGAGCTTTTGGGAAATTG+CGG + chr8_3:52402300-52402319 Msa1294600:intron 40.0%
GAAGCTTAAACATGAGGTAG+AGG + chr8_3:52402329-52402348 Msa1294600:intron 40.0%
! ACATCCTTTTATCGCGTGTT+TGG - chr8_3:52402375-52402394 None:intergenic 40.0%
! ATCGGATATGGAGTCCTTTT+AGG + chr8_3:52402409-52402428 Msa1294600:intron 40.0%
!! GATATGGAGTCCTTTTAGGT+TGG + chr8_3:52402413-52402432 Msa1294600:intron 40.0%
TTCAACGGATCCAACCTAAA+AGG - chr8_3:52402426-52402445 None:intergenic 40.0%
ATGAGAGAGATGGAGTCAAT+GGG - chr8_3:52402537-52402556 None:intergenic 40.0%
AATGAGAGAGATGGAGTCAA+TGG - chr8_3:52402538-52402557 None:intergenic 40.0%
AGCATAGCAGTAAAGGGATT+GGG - chr8_3:52402615-52402634 None:intergenic 40.0%
! ATGGAGAGCATAGCAGTAAA+GGG - chr8_3:52402621-52402640 None:intergenic 40.0%
TGAGCAAATCCAAAACCGAT+AGG + chr8_3:52402694-52402713 Msa1294600:intron 40.0%
!! ATTGATGATGCTGTCAACTG+TGG - chr8_3:52402768-52402787 None:intergenic 40.0%
AGGATTGTGGCATCATTTGA+AGG + chr8_3:52402795-52402814 Msa1294600:intron 40.0%
! TCAGCCACTTTGAAAGAGAA+AGG + chr8_3:52402893-52402912 Msa1294600:intron 40.0%
TGGACTGTGATAAGATTTGG+TGG + chr8_3:52402975-52402994 Msa1294600:intron 40.0%
! GGACTGTGATAAGATTTGGT+GGG + chr8_3:52402976-52402995 Msa1294600:intron 40.0%
ATCCTAGGGCCCATAAAAAA+TGG - chr8_3:52403059-52403078 None:intergenic 40.0%
CCATGGCTCAATTACTTGTA+TGG - chr8_3:52403149-52403168 None:intergenic 40.0%
CCATACAAGTAATTGAGCCA+TGG + chr8_3:52403146-52403165 Msa1294600:intron 40.0%
!! GCCATGGCATTATTTTGTCT+TGG + chr8_3:52403162-52403181 Msa1294600:intron 40.0%
CCCAAGACAAAATAATGCCA+TGG - chr8_3:52403166-52403185 None:intergenic 40.0%
TCAAATGCACTGTCTGTGTT+AGG + chr8_3:52403458-52403477 Msa1294600:intron 40.0%
GGTATATAAGTGTGCGCTTA+AGG + chr8_3:52403574-52403593 Msa1294600:intron 40.0%
AGGACTACAAATTGCTTGAG+CGG + chr8_3:52403673-52403692 Msa1294600:CDS 40.0%
ACATCGCCAATTATTGCTGT+TGG - chr8_3:52403717-52403736 None:intergenic 40.0%
! TACAATGGCAAAAGCATGAC+AGG - chr8_3:52403837-52403856 None:intergenic 40.0%
! GTAGTTCGCATTTTGTAGAC+AGG + chr8_3:52403854-52403873 Msa1294600:exon 40.0%
TGCATGACTGTACTTGACTA+AGG + chr8_3:52403966-52403985 Msa1294600:three_prime_UTR 40.0%
GCATGACTAATATTTAGGCC+TGG + chr8_3:52404015-52404034 Msa1294600:three_prime_UTR 40.0%
AATGATGAGTTTGTGAGGAG+TGG + chr8_3:52404044-52404063 Msa1294600:three_prime_UTR 40.0%
CGGTTACTTCTCTGAGTTTG+GGG - chr8_3:52400468-52400487 None:intergenic 45.0%
GCGGTTACTTCTCTGAGTTT+GGG - chr8_3:52400469-52400488 None:intergenic 45.0%
TGCGGTTACTTCTCTGAGTT+TGG - chr8_3:52400470-52400489 None:intergenic 45.0%
!!! GGGTTTTGGAGAGATAAGAG+AGG - chr8_3:52400540-52400559 None:intergenic 45.0%
!! AAGTGGGAGGTGATGGAAAT+GGG - chr8_3:52400584-52400603 None:intergenic 45.0%
!! AAAGTGGGAGGTGATGGAAA+TGG - chr8_3:52400585-52400604 None:intergenic 45.0%
GTTGAAAAAGTGGGAGGTGA+TGG - chr8_3:52400591-52400610 None:intergenic 45.0%
! ACAGCAACTTCAGGTTCTTG+TGG - chr8_3:52400860-52400879 None:intergenic 45.0%
TCAGTAGCTACAGCAACTTC+AGG - chr8_3:52400869-52400888 None:intergenic 45.0%
! TCTCTCTGAGGGAACTAAAC+AGG + chr8_3:52400943-52400962 Msa1294600:CDS 45.0%
GCCAAGACTGCAGAAATACA+AGG + chr8_3:52401183-52401202 Msa1294600:intron 45.0%
! GCCTTGTATTTCTGCAGTCT+TGG - chr8_3:52401187-52401206 None:intergenic 45.0%
GGAGCTGACATTACAGTTGT+AGG + chr8_3:52401644-52401663 Msa1294600:CDS 45.0%
AGATAAATGCCAACGGATGC+TGG - chr8_3:52401850-52401869 None:intergenic 45.0%
AAGAGCCAAGTGTGTGTTAG+GGG + chr8_3:52402247-52402266 Msa1294600:intron 45.0%
!! AGCTTTTGGGAAATTGCGGT+GGG + chr8_3:52402304-52402323 Msa1294600:intron 45.0%
TGGGAGGAAGCTTAAACATG+AGG + chr8_3:52402323-52402342 Msa1294600:intron 45.0%
!! TCAATGGGTTAGATCTGGTG+TGG - chr8_3:52402522-52402541 None:intergenic 45.0%
!! TGGAGTCAATGGGTTAGATC+TGG - chr8_3:52402527-52402546 None:intergenic 45.0%
GAGCATAGCAGTAAAGGGAT+TGG - chr8_3:52402616-52402635 None:intergenic 45.0%
! CATGGAGAGCATAGCAGTAA+AGG - chr8_3:52402622-52402641 None:intergenic 45.0%
! GGTAATGGTAGGCTTCCTAT+CGG - chr8_3:52402712-52402731 None:intergenic 45.0%
!! CATGCTTGCTTGGTAATGGT+AGG - chr8_3:52402723-52402742 None:intergenic 45.0%
!!! GTCCATTTTTTATGGGCCCT+AGG + chr8_3:52403054-52403073 Msa1294600:intron 45.0%
GAGTGAGTGAAGAGCAAAAG+AGG + chr8_3:52403200-52403219 Msa1294600:intron 45.0%
TGAAGAGCAAAAGAGGACGA+AGG + chr8_3:52403207-52403226 Msa1294600:intron 45.0%
!! GAAAAATGCAGGTGGGTTGA+AGG + chr8_3:52403653-52403672 Msa1294600:intron 45.0%
TACAAATTGCTTGAGCGGCT+TGG + chr8_3:52403678-52403697 Msa1294600:CDS 45.0%
! GAGTTTGTGAGGAGTGGATT+TGG + chr8_3:52404050-52404069 Msa1294600:three_prime_UTR 45.0%
! CAATGTGGGCCTCCTATTTT+AGG + chr8_3:52404115-52404134 Msa1294600:three_prime_UTR 45.0%
!!! ATTTATTTTTTCTTTTTAAA+AGG + chr8_3:52403293-52403312 Msa1294600:intron 5.0%
GAAGTAACCGCAAGCATCCA+TGG + chr8_3:52400478-52400497 Msa1294600:exon 50.0%
ATGAACGCCATGGATGCTTG+CGG - chr8_3:52400488-52400507 None:intergenic 50.0%
! GAGGGTTTGACGAGTTGAAG+CGG - chr8_3:52400521-52400540 None:intergenic 50.0%
GGAAGGGGGGTTGAAAAAGT+GGG - chr8_3:52400600-52400619 None:intergenic 50.0%
GCTATCTTGGACAGTCTCAC+AGG - chr8_3:52401376-52401395 None:intergenic 50.0%
GATAGTTGGGTCTCATGCTC+AGG - chr8_3:52401828-52401847 None:intergenic 50.0%
CCAACGGATGCTGGATAGTT+GGG - chr8_3:52401841-52401860 None:intergenic 50.0%
TCACACCCCTAACACACACT+TGG - chr8_3:52402255-52402274 None:intergenic 50.0%
!! GAGCTTTTGGGAAATTGCGG+TGG + chr8_3:52402303-52402322 Msa1294600:intron 50.0%
!! TTTTGGGAAATTGCGGTGGG+AGG + chr8_3:52402307-52402326 Msa1294600:intron 50.0%
! CGCGTGTTTGGTAATCCACT+GGG - chr8_3:52402363-52402382 None:intergenic 50.0%
! TCGCGTGTTTGGTAATCCAC+TGG - chr8_3:52402364-52402383 None:intergenic 50.0%
GAGAGAGGCAATGAGAGAGA+TGG - chr8_3:52402547-52402566 None:intergenic 50.0%
ACTTGCTTCAGCCTTGCACA+TGG - chr8_3:52402656-52402675 None:intergenic 50.0%
!!! GGTAGGCTTCCTATCGGTTT+TGG - chr8_3:52402706-52402725 None:intergenic 50.0%
GATGCTGTCAACTGTGGCTT+TGG - chr8_3:52402762-52402781 None:intergenic 50.0%
GGGCCCTAGGATATGATATC+TGG + chr8_3:52403067-52403086 Msa1294600:intron 50.0%
AGAGCAAAAGAGGACGAAGG+AGG + chr8_3:52403210-52403229 Msa1294600:intron 50.0%
CAAAAGAGGACGAAGGAGGA+AGG + chr8_3:52403214-52403233 Msa1294600:intron 50.0%
AGGAAGGACGAGGGAGATAA+GGG + chr8_3:52403230-52403249 Msa1294600:intron 50.0%
! GGCAAAAGCATGACAGGACA+GGG - chr8_3:52403831-52403850 None:intergenic 50.0%
! TGGCAAAAGCATGACAGGAC+AGG - chr8_3:52403832-52403851 None:intergenic 50.0%
TGGCAGAGTCATGAACGCCA+TGG - chr8_3:52400498-52400517 None:intergenic 55.0%
! GGTTTGACGAGTTGAAGCGG+TGG - chr8_3:52400518-52400537 None:intergenic 55.0%
AGGGGGGTTGAAAAAGTGGG+AGG - chr8_3:52400597-52400616 None:intergenic 55.0%
GGGAAGGGGGGTTGAAAAAG+TGG - chr8_3:52400601-52400620 None:intergenic 55.0%
ACCAATGATCCTGGTGGGGA+GGG - chr8_3:52400633-52400652 None:intergenic 55.0%
TACCAATGATCCTGGTGGGG+AGG - chr8_3:52400634-52400653 None:intergenic 55.0%
CCCAACTATCCAGCATCCGT+TGG + chr8_3:52401838-52401857 Msa1294600:CDS 55.0%
GCCAACGGATGCTGGATAGT+TGG - chr8_3:52401842-52401861 None:intergenic 55.0%
! TAGGGGTGTGACCGCAAAGT+AGG + chr8_3:52402264-52402283 Msa1294600:intron 55.0%
GGTGTGACCGCAAAGTAGGT+TGG + chr8_3:52402268-52402287 Msa1294600:intron 55.0%
GTAGAGGTGATGTGACCCAG+TGG + chr8_3:52402345-52402364 Msa1294600:intron 55.0%
GCAGTAAAGGGATTGGGAGC+AGG - chr8_3:52402609-52402628 None:intergenic 55.0%
GAGGAAGGACGAGGGAGATA+AGG + chr8_3:52403229-52403248 Msa1294600:intron 55.0%
!! TTGCTTGAGCGGCTTGGTGA+TGG + chr8_3:52403684-52403703 Msa1294600:CDS 55.0%
!! TGCTTGAGCGGCTTGGTGAT+GGG + chr8_3:52403685-52403704 Msa1294600:CDS 55.0%
AGGGTGTGAAGTCGGGAAGG+GGG - chr8_3:52400614-52400633 None:intergenic 60.0%
! GAGGGTGTGAAGTCGGGAAG+GGG - chr8_3:52400615-52400634 None:intergenic 60.0%
! GGAGGGTGTGAAGTCGGGAA+GGG - chr8_3:52400616-52400635 None:intergenic 60.0%
! TGGTGGGGAGGGTGTGAAGT+CGG - chr8_3:52400622-52400641 None:intergenic 60.0%
ACCCTCCCCACCAGGATCAT+TGG + chr8_3:52400629-52400648 Msa1294600:CDS 60.0%
AGGACGAAGGAGGAAGGACG+AGG + chr8_3:52403220-52403239 Msa1294600:intron 60.0%
GGACGAAGGAGGAAGGACGA+GGG + chr8_3:52403221-52403240 Msa1294600:intron 60.0%
CGAGGGAGATAAGGGCCACT+CGG + chr8_3:52403238-52403257 Msa1294600:intron 60.0%
GAGGGAGATAAGGGCCACTC+GGG + chr8_3:52403239-52403258 Msa1294600:intron 60.0%
AGGGAGATAAGGGCCACTCG+GGG + chr8_3:52403240-52403259 Msa1294600:intron 60.0%
GGGTGTGAAGTCGGGAAGGG+GGG - chr8_3:52400613-52400632 None:intergenic 65.0%
! GGGAGGGTGTGAAGTCGGGA+AGG - chr8_3:52400617-52400636 None:intergenic 65.0%
! GGTGGGGAGGGTGTGAAGTC+GGG - chr8_3:52400621-52400640 None:intergenic 65.0%
GACTTCACACCCTCCCCACC+AGG + chr8_3:52400621-52400640 Msa1294600:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 52400313 52404293 52400313 ID=Msa1294600;Name=Msa1294600
chr8_3 mRNA 52400313 52404293 52400313 ID=Msa1294600-mRNA-1;Parent=Msa1294600;Name=Msa1294600-mRNA-1;_AED=0.17;_eAED=0.17;_QI=184|1|1|1|1|1|5|436|200
chr8_3 exon 52400313 52400650 52400313 ID=Msa1294600-mRNA-1:exon:14902;Parent=Msa1294600-mRNA-1
chr8_3 exon 52400846 52400964 52400846 ID=Msa1294600-mRNA-1:exon:14903;Parent=Msa1294600-mRNA-1
chr8_3 exon 52401608 52401665 52401608 ID=Msa1294600-mRNA-1:exon:14904;Parent=Msa1294600-mRNA-1
chr8_3 exon 52401796 52401873 52401796 ID=Msa1294600-mRNA-1:exon:14905;Parent=Msa1294600-mRNA-1
chr8_3 exon 52403664 52404293 52403664 ID=Msa1294600-mRNA-1:exon:14906;Parent=Msa1294600-mRNA-1
chr8_3 five_prime_UTR 52400313 52400496 52400313 ID=Msa1294600-mRNA-1:five_prime_utr;Parent=Msa1294600-mRNA-1
chr8_3 CDS 52400497 52400650 52400497 ID=Msa1294600-mRNA-1:cds;Parent=Msa1294600-mRNA-1
chr8_3 CDS 52400846 52400964 52400846 ID=Msa1294600-mRNA-1:cds;Parent=Msa1294600-mRNA-1
chr8_3 CDS 52401608 52401665 52401608 ID=Msa1294600-mRNA-1:cds;Parent=Msa1294600-mRNA-1
chr8_3 CDS 52401796 52401873 52401796 ID=Msa1294600-mRNA-1:cds;Parent=Msa1294600-mRNA-1
chr8_3 CDS 52403664 52403857 52403664 ID=Msa1294600-mRNA-1:cds;Parent=Msa1294600-mRNA-1
chr8_3 three_prime_UTR 52403858 52404293 52403858 ID=Msa1294600-mRNA-1:three_prime_utr;Parent=Msa1294600-mRNA-1
Gene Sequence

>Msa1294600

ATGGCGTTCATGACTCTGCCACCGCTTCAACTCGTCAAACCCTCTCTTATCTCTCCAAAACCCTCCCTATTCTTCAATAACACACCCATTTCCATCACCTCCCACTTTTTCAACCCCCCTTCCCGACTTCACACCCTCCCCACCAGGATCATTGTTAAGGCTAAACCACAAGAACCTGAAGTTGCTGTAGCTACTGATTCATTTACACAATTCAATCATCTTCTTTTGCCTATTACTGATAGAAAACCCTATCTCTCTGAGGGAACTAAACAGGCTATAGCGACTACCATTGCCTTAGCTAACAAGTATGGAGCTGACATTACAGTTGTAGTTATCGATGAACAGCAGAAGGAGTCACTGCCTGAGCATGAGACCCAACTATCCAGCATCCGTTGGCATTTATCTGAAGGTGGGTTGAAGGACTACAAATTGCTTGAGCGGCTTGGTGATGGGAGTAAGCCAACAGCAATAATTGGCGATGTTGCTGATGAACTTAATTTGGATTTGGTAGTTATTAGCATGGAAGCAATTCATACAAAGCATATAGATGCAAATTTGCTTGCTGAGTTCATTCCCTGTCCTGTCATGCTTTTGCCATTGTAG
Protein sequence

>Msa1294600

MAFMTLPPLQLVKPSLISPKPSLFFNNTPISITSHFFNPPSRLHTLPTRIIVKAKPQEPEVAVATDSFTQFNHLLLPITDRKPYLSEGTKQAIATTIALANKYGADITVVVIDEQQKESLPEHETQLSSIRWHLSEGGLKDYKLLERLGDGSKPTAIIGDVADELNLDLVVISMEAIHTKHIDANLLAEFIPCPVMLLPL*