AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1254360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1254360 MtrunA17_Chr8g0371391 95.522 201 8 1 1 200 1 201 1.70e-137 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1254360 AT5G66090.1 66.258 163 49 2 43 200 49 210 2.02e-75 226

Find 84 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATATATTGTAATCAATTT+TGG 0.136663 8_2:+52505432 Msa1254360:three_prime_UTR
TGTTGCTGATGAACTTAATT+TGG 0.180753 8_2:+52504904 Msa1254360:CDS
ATATGCTACTCTAAAGTTTC+AGG 0.183780 8_2:+52505097 Msa1254360:three_prime_UTR
TGCGGTTACTTCTCTGAGTT+TGG 0.226890 8_2:-52501626 None:intergenic
TTGAAGAATAGGGAGGGTTT+TGG 0.277998 8_2:-52501710 None:intergenic
AAACTCATCATTTCCTTTCC+AGG 0.296477 8_2:-52505203 None:intergenic
CTGAAGCATGACTAATATTT+AGG 0.306734 8_2:+52505180 Msa1254360:three_prime_UTR
TTTAGTTCCCTCAGAGAGAT+AGG 0.318311 8_2:-52502098 None:intergenic
GAGTTTGTGAGGAGTGGATT+TGG 0.319040 8_2:+52505220 Msa1254360:three_prime_UTR
AGCAAGCCAACAGCAATAAT+TGG 0.328721 8_2:+52504878 Msa1254360:CDS
GCGGTTACTTCTCTGAGTTT+GGG 0.329354 8_2:-52501625 None:intergenic
GAGGGTGTGAAGTCGGGAAG+GGG 0.335959 8_2:-52501771 None:intergenic
AGGGTGTGAAGTCGGGAAGG+GGG 0.337342 8_2:-52501770 None:intergenic
AATTATACACAACCTAAAAT+AGG 0.338514 8_2:-52505297 None:intergenic
TCAAAACACTAATGTTGGTT+TGG 0.369198 8_2:+52505344 Msa1254360:three_prime_UTR
TTTATCAATTTCGTTGTTTG+AGG 0.383976 8_2:-52501559 None:intergenic
GGGTGTGAAGTCGGGAAGGG+GGG 0.386173 8_2:-52501769 None:intergenic
TGGGTGTGTTATTGAAGAAT+AGG 0.393378 8_2:-52501721 None:intergenic
GGTGGGGAGGGTGTGAAGTC+GGG 0.401871 8_2:-52501777 None:intergenic
TGATGAACTTAATTTGGATT+TGG 0.404360 8_2:+52504910 Msa1254360:CDS
ATTGCCTTAGCTAACAAGTA+TGG 0.418236 8_2:+52502795 Msa1254360:CDS
ACCCTCCCCACCAGGATCAT+TGG 0.426256 8_2:+52501788 Msa1254360:CDS
TCTCTCTGAGGGAACTAAAC+AGG 0.436554 8_2:+52502102 Msa1254360:CDS
GGGTGTGTTATTGAAGAATA+GGG 0.438335 8_2:-52501720 None:intergenic
AGATAAATGCCAGCGGATGC+TGG 0.444524 8_2:-52503019 None:intergenic
TCAGTAGCTACAGCAACTTC+AGG 0.444631 8_2:-52502025 None:intergenic
AAGTGGGAGGTGATGGAAAT+GGG 0.445737 8_2:-52501740 None:intergenic
TACAAATTGCTTGAGCGGCT+TGG 0.450205 8_2:+52504848 Msa1254360:CDS
GCCAGCGGATGCTGGATAGT+TGG 0.455374 8_2:-52503011 None:intergenic
GATAGTTGGGTCTCATGCTC+AGG 0.456507 8_2:-52502997 None:intergenic
GGGAGGGTGTGAAGTCGGGA+AGG 0.458649 8_2:-52501773 None:intergenic
CCAGCGGATGCTGGATAGTT+GGG 0.459161 8_2:-52503010 None:intergenic
GGAGGGTGTGAAGTCGGGAA+GGG 0.463065 8_2:-52501772 None:intergenic
TTGCTTGAGCGGCTTGGTGA+TGG 0.467825 8_2:+52504854 Msa1254360:CDS
ATTATTCAAAACACTAATGT+TGG 0.468386 8_2:+52505339 Msa1254360:three_prime_UTR
AAAGTGGGAGGTGATGGAAA+TGG 0.468946 8_2:-52501741 None:intergenic
GGAGCTGACATTACAGTTGT+AGG 0.469978 8_2:+52502816 Msa1254360:CDS
TGGCAAAAGCATGACAGGAC+AGG 0.472545 8_2:-52504999 None:intergenic
ACATCGCCAATTATTGCTGT+TGG 0.484894 8_2:-52504884 None:intergenic
ACCAATGATCCTGGTGGGGA+GGG 0.491868 8_2:-52501789 None:intergenic
TATGCTACTCTAAAGTTTCA+GGG 0.507896 8_2:+52505098 Msa1254360:three_prime_UTR
TGCTTGAGCGGCTTGGTGAT+GGG 0.512598 8_2:+52504855 Msa1254360:CDS
TGGTGGGGAGGGTGTGAAGT+CGG 0.513130 8_2:-52501778 None:intergenic
ATCCGCTGGCATTTATCTGA+AGG 0.515115 8_2:+52503024 Msa1254360:CDS
GACTTCACACCCTCCCCACC+AGG 0.519206 8_2:+52501780 Msa1254360:CDS
TACAATGGCAAAAGCATGAC+AGG 0.519854 8_2:-52505004 None:intergenic
TTAGTTCCCTCAGAGAGATA+GGG 0.523045 8_2:-52502097 None:intergenic
AATGATGAGTTTGTGAGGAG+TGG 0.527912 8_2:+52505214 Msa1254360:three_prime_UTR
CCCAACTATCCAGCATCCGC+TGG 0.529801 8_2:+52503010 Msa1254360:CDS
AGTTATATTACCAATGATCC+TGG 0.538740 8_2:-52501798 None:intergenic
GAAGTAACCGCAAGCATCCA+TGG 0.542093 8_2:+52501637 Msa1254360:exon
AGCTCCATACTTGTTAGCTA+AGG 0.544707 8_2:-52502799 None:intergenic
TGCATGACTGTACTTGACTA+AGG 0.547431 8_2:+52505136 Msa1254360:three_prime_UTR
GTGTTATTGAAGAATAGGGA+GGG 0.550609 8_2:-52501716 None:intergenic
ACAGCAACTTCAGGTTCTTG+TGG 0.551790 8_2:-52502016 None:intergenic
ACTAATATTTAGGCCTGGAA+AGG 0.553070 8_2:+52505190 Msa1254360:three_prime_UTR
GCATGACTAATATTTAGGCC+TGG 0.555476 8_2:+52505185 Msa1254360:three_prime_UTR
CGGTTACTTCTCTGAGTTTG+GGG 0.556267 8_2:-52501624 None:intergenic
ATATTACCAATGATCCTGGT+GGG 0.557905 8_2:-52501794 None:intergenic
CTACTAGTATCAAACAATGT+GGG 0.560265 8_2:+52505271 Msa1254360:three_prime_UTR
TACCAATGATCCTGGTGGGG+AGG 0.564673 8_2:-52501790 None:intergenic
GGATTTGGTAGTTATTAGCA+TGG 0.566741 8_2:+52504925 Msa1254360:CDS
CAAGATTCTAGTGACTTCCA+CGG 0.575836 8_2:-52501589 None:intergenic
AAGATTCTAGTGACTTCCAC+GGG 0.576868 8_2:-52501588 None:intergenic
AGGACTACAAATTGCTTGAG+CGG 0.586994 8_2:+52504843 Msa1254360:CDS
CTACAAAATGCGAACTACAA+TGG 0.587815 8_2:-52505019 None:intergenic
GGCAAAAGCATGACAGGACA+GGG 0.588085 8_2:-52504998 None:intergenic
TATACACAACCTAAAATAGG+AGG 0.600243 8_2:-52505294 None:intergenic
AGAAAACCCTATCTCTCTGA+GGG 0.601139 8_2:+52502091 Msa1254360:CDS
GATTTGGATTCTTTCATGTG+AGG 0.606825 8_2:+52505236 Msa1254360:three_prime_UTR
AGTTATCGATGAACAGCAGA+AGG 0.615289 8_2:+52502966 Msa1254360:intron
GAGGGTTTGACGAGTTGAAG+CGG 0.621708 8_2:-52501677 None:intergenic
TCTACTAGTATCAAACAATG+TGG 0.623417 8_2:+52505270 Msa1254360:three_prime_UTR
TATTACCAATGATCCTGGTG+GGG 0.632686 8_2:-52501793 None:intergenic
TGGCAGAGTCATGAACGCCA+TGG 0.640926 8_2:-52501654 None:intergenic
ATACTTGTTAGCTAAGGCAA+TGG 0.644889 8_2:-52502793 None:intergenic
TGTGTTATTGAAGAATAGGG+AGG 0.650429 8_2:-52501717 None:intergenic
TAGAAAACCCTATCTCTCTG+AGG 0.651248 8_2:+52502090 Msa1254360:CDS
TATATTACCAATGATCCTGG+TGG 0.656745 8_2:-52501795 None:intergenic
ATGAACGCCATGGATGCTTG+CGG 0.669244 8_2:-52501644 None:intergenic
AAGGAAATGATGAGTTTGTG+AGG 0.675607 8_2:+52505209 Msa1254360:three_prime_UTR
AATTGATAAAACGAAACCCG+TGG 0.683798 8_2:+52501572 Msa1254360:five_prime_UTR
GGTTTGACGAGTTGAAGCGG+TGG 0.698692 8_2:-52501674 None:intergenic
AACCTTCAGATAAATGCCAG+CGG 0.701135 8_2:-52503026 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATTATCTTTTTGTT+AGG + chr8_2:52502758-52502777 Msa1254360:intron 10.0%
!!! TATGTAAATATTTTTGTTAT+TGG + chr8_2:52504031-52504050 Msa1254360:intron 10.0%
!!! TAGTAATTTTACGTAATTTA+TGG - chr8_2:52501502-52501521 None:intergenic 15.0%
!! AAGAAACTATAATAATTTCT+TGG - chr8_2:52501850-52501869 None:intergenic 15.0%
!!! CTTTTTCTTTTCAATTTATT+TGG + chr8_2:52502130-52502149 Msa1254360:intron 15.0%
!! TAAGAATTTAGATTCTATAT+GGG - chr8_2:52502395-52502414 None:intergenic 15.0%
!! TTAAGAATTTAGATTCTATA+TGG - chr8_2:52502396-52502415 None:intergenic 15.0%
!! AAATTCATAATATCAAAAAG+AGG - chr8_2:52502870-52502889 None:intergenic 15.0%
!!! TGATATTATGAATTTTTTAC+TGG + chr8_2:52502875-52502894 Msa1254360:intron 15.0%
!!! GATATTATGAATTTTTTACT+GGG + chr8_2:52502876-52502895 Msa1254360:intron 15.0%
!!! ATATTATGAATTTTTTACTG+GGG + chr8_2:52502877-52502896 Msa1254360:intron 15.0%
!!! ATTTTTTCTTTTTAAAAGGT+TGG + chr8_2:52504467-52504486 Msa1254360:intron 15.0%
!! TATTTAATAATGAAAAATGC+AGG + chr8_2:52504812-52504831 Msa1254360:intron 15.0%
!!! CTATATATTGTAATCAATTT+TGG + chr8_2:52505432-52505451 Msa1254360:three_prime_UTR 15.0%
!!! TTGATTTTGTAGAAGTTTTT+AGG - chr8_2:52502582-52502601 None:intergenic 20.0%
!!! AATCTAATAATGGAAAAAAG+TGG - chr8_2:52503637-52503656 None:intergenic 20.0%
!! TGGATGATTTAATCTAATAA+TGG - chr8_2:52503647-52503666 None:intergenic 20.0%
!!! TTTTTTTGCTTCTCTTTTTT+TGG + chr8_2:52504001-52504020 Msa1254360:intron 20.0%
!!! CCAATGTTTTATTCAAAAAA+CGG - chr8_2:52504565-52504584 None:intergenic 20.0%
!!! CATTTTTAAGTAAACATTCA+TGG + chr8_2:52504723-52504742 Msa1254360:intron 20.0%
!! AATTATACACAACCTAAAAT+AGG - chr8_2:52505300-52505319 None:intergenic 20.0%
!! ATTATTCAAAACACTAATGT+TGG + chr8_2:52505339-52505358 Msa1254360:three_prime_UTR 20.0%
! TTTATCAATTTCGTTGTTTG+AGG - chr8_2:52501562-52501581 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAAAC+AGG + chr8_2:52502679-52502698 Msa1254360:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + chr8_2:52502680-52502699 Msa1254360:intron 25.0%
!!! ATTTGTTGAGTTTTTTTGTG+TGG + chr8_2:52503074-52503093 Msa1254360:intron 25.0%
! AAACTAGTAAAGTGAAAAGT+AGG + chr8_2:52503133-52503152 Msa1254360:intron 25.0%
! TTATTTGTAATGTACCTTCA+AGG - chr8_2:52503285-52503304 None:intergenic 25.0%
! ATTTGCTCATTGTAAAAAAC+AGG - chr8_2:52503853-52503872 None:intergenic 25.0%
!!! TAGAGTTGATGAGTTAAATT+GGG + chr8_2:52504116-52504135 Msa1254360:intron 25.0%
! TAATATGACAGTGTAAAAAG+TGG + chr8_2:52504175-52504194 Msa1254360:intron 25.0%
!!! TTTTAAGGGTCCATTTTTTA+TGG + chr8_2:52504216-52504235 Msa1254360:intron 25.0%
!!! TTTAAGGGTCCATTTTTTAT+GGG + chr8_2:52504217-52504236 Msa1254360:intron 25.0%
!! ATTGAGTAACAGATTTTAAG+TGG + chr8_2:52504498-52504517 Msa1254360:intron 25.0%
! ATAAGACATAGTAAAGGATA+AGG - chr8_2:52504535-52504554 None:intergenic 25.0%
!!! CCGTTTTTTGAATAAAACAT+TGG + chr8_2:52504562-52504581 Msa1254360:intron 25.0%
!!! TTTGATTTGCTACAAAATGA+AGG - chr8_2:52504613-52504632 None:intergenic 25.0%
! TTAATAATGAAAAATGCAGG+TGG + chr8_2:52504815-52504834 Msa1254360:intron 25.0%
! TAATAATGAAAAATGCAGGT+GGG + chr8_2:52504816-52504835 Msa1254360:intron 25.0%
!! TGATGAACTTAATTTGGATT+TGG + chr8_2:52504910-52504929 Msa1254360:CDS 25.0%
AGTTATATTACCAATGATCC+TGG - chr8_2:52501801-52501820 None:intergenic 30.0%
TTTCTTTGATGCATGCAATT+TGG + chr8_2:52501864-52501883 Msa1254360:intron 30.0%
TTCTTTGATGCATGCAATTT+GGG + chr8_2:52501865-52501884 Msa1254360:intron 30.0%
GTTTCATCTGAATTCAGTTA+AGG + chr8_2:52501988-52502007 Msa1254360:intron 30.0%
! TAGGGTTTTCTATCAGTAAT+AGG - chr8_2:52502082-52502101 None:intergenic 30.0%
CAATTTCATTAACGAAAGTC+TGG + chr8_2:52502319-52502338 Msa1254360:intron 30.0%
TTATGAACAATGTCATAAGC+TGG - chr8_2:52502498-52502517 None:intergenic 30.0%
! GGTATTTTCATAAGCTATCT+TGG - chr8_2:52502561-52502580 None:intergenic 30.0%
GTTCTTGTATGAAGTTGTAA+TGG + chr8_2:52502899-52502918 Msa1254360:intron 30.0%
!!! TGAGTTTTTTTGTGTGGATT+TGG + chr8_2:52503080-52503099 Msa1254360:intron 30.0%
! CTTTACTAGTTTACTCTCAA+CGG - chr8_2:52503125-52503144 None:intergenic 30.0%
CTCATGATTGCTTATGTATT+TGG + chr8_2:52503178-52503197 Msa1254360:intron 30.0%
ATGATTGCTTATGTATTTGG+AGG + chr8_2:52503181-52503200 Msa1254360:intron 30.0%
!! TCCATTGATTTATTCGTCAA+TGG + chr8_2:52503212-52503231 Msa1254360:intron 30.0%
ACCATTGACGAATAAATCAA+TGG - chr8_2:52503216-52503235 None:intergenic 30.0%
ATGTACACACAGTGAATTAT+CGG + chr8_2:52503561-52503580 Msa1254360:intron 30.0%
ATCATGGATCAATACTTCAA+CGG - chr8_2:52503611-52503630 None:intergenic 30.0%
AGAAAGAAAATATGAGAGAG+AGG - chr8_2:52503732-52503751 None:intergenic 30.0%
ATCAATTTCTACTAGGATTG+TGG + chr8_2:52503952-52503971 Msa1254360:intron 30.0%
!! GTAGAGTTGATGAGTTAAAT+TGG + chr8_2:52504115-52504134 Msa1254360:intron 30.0%
TGAGTTAAATTGGGTTTAGT+TGG + chr8_2:52504125-52504144 Msa1254360:intron 30.0%
GTGTAAAAAGTGGTATATCA+AGG + chr8_2:52504185-52504204 Msa1254360:intron 30.0%
TCTTACCCAAGACAAAATAA+TGG - chr8_2:52504341-52504360 None:intergenic 30.0%
! ATTTTGTCTTGGGTAAGAAA+AGG + chr8_2:52504343-52504362 Msa1254360:intron 30.0%
CATGCAATAAGACATAGTAA+AGG - chr8_2:52504541-52504560 None:intergenic 30.0%
TGTTGCTGATGAACTTAATT+TGG + chr8_2:52504904-52504923 Msa1254360:CDS 30.0%
!! ATATGCTACTCTAAAGTTTC+AGG + chr8_2:52505097-52505116 Msa1254360:three_prime_UTR 30.0%
!! TATGCTACTCTAAAGTTTCA+GGG + chr8_2:52505098-52505117 Msa1254360:three_prime_UTR 30.0%
CTGAAGCATGACTAATATTT+AGG + chr8_2:52505180-52505199 Msa1254360:three_prime_UTR 30.0%
TCTACTAGTATCAAACAATG+TGG + chr8_2:52505270-52505289 Msa1254360:three_prime_UTR 30.0%
CTACTAGTATCAAACAATGT+GGG + chr8_2:52505271-52505290 Msa1254360:three_prime_UTR 30.0%
TATACACAACCTAAAATAGG+AGG - chr8_2:52505297-52505316 None:intergenic 30.0%
! TCAAAACACTAATGTTGGTT+TGG + chr8_2:52505344-52505363 Msa1254360:three_prime_UTR 30.0%
AATTGATAAAACGAAACCCG+TGG + chr8_2:52501572-52501591 Msa1254360:five_prime_UTR 35.0%
! GTGTTATTGAAGAATAGGGA+GGG - chr8_2:52501719-52501738 None:intergenic 35.0%
! TGTGTTATTGAAGAATAGGG+AGG - chr8_2:52501720-52501739 None:intergenic 35.0%
! GGGTGTGTTATTGAAGAATA+GGG - chr8_2:52501723-52501742 None:intergenic 35.0%
! TGGGTGTGTTATTGAAGAAT+AGG - chr8_2:52501724-52501743 None:intergenic 35.0%
ATATTACCAATGATCCTGGT+GGG - chr8_2:52501797-52501816 None:intergenic 35.0%
TATATTACCAATGATCCTGG+TGG - chr8_2:52501798-52501817 None:intergenic 35.0%
ACTGATAAACACCTTGACTT+AGG + chr8_2:52502207-52502226 Msa1254360:intron 35.0%
TAGCTAAATAATGAGCAGCT+TGG + chr8_2:52502425-52502444 Msa1254360:intron 35.0%
TAAATAATGAGCAGCTTGGT+TGG + chr8_2:52502429-52502448 Msa1254360:intron 35.0%
CCTAACATGCAAAACTGTTT+GGG + chr8_2:52502459-52502478 Msa1254360:intron 35.0%
!!! AACCACAAGTCAGTGTTTTT+TGG - chr8_2:52502729-52502748 None:intergenic 35.0%
! ATACTTGTTAGCTAAGGCAA+TGG - chr8_2:52502796-52502815 None:intergenic 35.0%
ATTGCCTTAGCTAACAAGTA+TGG + chr8_2:52502795-52502814 Msa1254360:CDS 35.0%
GTTGATATTACTCGCTTTAG+AGG + chr8_2:52503234-52503253 Msa1254360:intron 35.0%
!!! ACTGTGTCCTTTTTTTGTTG+AGG + chr8_2:52503258-52503277 Msa1254360:intron 35.0%
TCAAGGACCTCAACAAAAAA+AGG - chr8_2:52503268-52503287 None:intergenic 35.0%
!!! TTTTTTGTTGAGGTCCTTGA+AGG + chr8_2:52503268-52503287 Msa1254360:intron 35.0%
!! GTTCAACTACTATCGATTTG+TGG + chr8_2:52503369-52503388 Msa1254360:intron 35.0%
! AGCTAATACTTGGAGCTTTT+GGG + chr8_2:52503461-52503480 Msa1254360:intron 35.0%
ATTACCAAACACGCGATAAA+AGG + chr8_2:52503538-52503557 Msa1254360:intron 35.0%
ACACAGTGAATTATCGGATA+TGG + chr8_2:52503567-52503586 Msa1254360:intron 35.0%
TGGAAAAAAGTGGTCTATCA+TGG - chr8_2:52503627-52503646 None:intergenic 35.0%
CATCCAACCAAACTTTATCA+TGG + chr8_2:52503661-52503680 Msa1254360:intron 35.0%
AGACCATGATAAAGTTTGGT+TGG - chr8_2:52503667-52503686 None:intergenic 35.0%
GGTTAGACCATGATAAAGTT+TGG - chr8_2:52503671-52503690 None:intergenic 35.0%
! CACATGGTATTTCTATGACA+TGG - chr8_2:52503810-52503829 None:intergenic 35.0%
TCATAGAAATACCATGTGCA+AGG + chr8_2:52503812-52503831 Msa1254360:intron 35.0%
TGCTCATTGTAAAAAACAGG+TGG - chr8_2:52503850-52503869 None:intergenic 35.0%
ATTACATGCTTGCTTGGTAA+TGG - chr8_2:52503897-52503916 None:intergenic 35.0%
! TTTTGGATTACATGCTTGCT+TGG - chr8_2:52503903-52503922 None:intergenic 35.0%
!!! TGTGGCTTTGGTTTTCATTT+TGG - chr8_2:52503920-52503939 None:intergenic 35.0%
CAGCATCATCAATTTCTACT+AGG + chr8_2:52503945-52503964 Msa1254360:intron 35.0%
AGTTCCTTTCTCTTTCAAAG+TGG - chr8_2:52504070-52504089 None:intergenic 35.0%
AGTTGGACTGTGATAAGATT+TGG + chr8_2:52504142-52504161 Msa1254360:intron 35.0%
! ATCAAGGACCAGTCTTTTTA+AGG + chr8_2:52504201-52504220 Msa1254360:intron 35.0%
! TCAAGGACCAGTCTTTTTAA+GGG + chr8_2:52504202-52504221 Msa1254360:intron 35.0%
AAATGGACCCTTAAAAAGAC+TGG - chr8_2:52504212-52504231 None:intergenic 35.0%
ACACCAGATATCATATCCTA+GGG - chr8_2:52504243-52504262 None:intergenic 35.0%
AACACCAGATATCATATCCT+AGG - chr8_2:52504244-52504263 None:intergenic 35.0%
!! CCATGCCATTATTTTGTCTT+GGG + chr8_2:52504333-52504352 Msa1254360:intron 35.0%
TAATAAAATTCATCCCCGAG+TGG - chr8_2:52504426-52504445 None:intergenic 35.0%
! ACTCGGGGATGAATTTTATT+AGG + chr8_2:52504425-52504444 Msa1254360:intron 35.0%
! GGATTTGGTAGTTATTAGCA+TGG + chr8_2:52504925-52504944 Msa1254360:CDS 35.0%
CTACAAAATGCGAACTACAA+TGG - chr8_2:52505022-52505041 None:intergenic 35.0%
! TAGTTCGCATTTTGTAGACA+GGG + chr8_2:52505025-52505044 Msa1254360:exon 35.0%
ACTAATATTTAGGCCTGGAA+AGG + chr8_2:52505190-52505209 Msa1254360:three_prime_UTR 35.0%
AAACTCATCATTTCCTTTCC+AGG - chr8_2:52505206-52505225 None:intergenic 35.0%
AAGGAAATGATGAGTTTGTG+AGG + chr8_2:52505209-52505228 Msa1254360:three_prime_UTR 35.0%
GATTTGGATTCTTTCATGTG+AGG + chr8_2:52505236-52505255 Msa1254360:three_prime_UTR 35.0%
AAGATTCTAGTGACTTCCAC+GGG - chr8_2:52501591-52501610 None:intergenic 40.0%
CAAGATTCTAGTGACTTCCA+CGG - chr8_2:52501592-52501611 None:intergenic 40.0%
!!! GGTTTTGGAGAGATAAGAGA+GGG - chr8_2:52501698-52501717 None:intergenic 40.0%
! TTGAAGAATAGGGAGGGTTT+TGG - chr8_2:52501713-52501732 None:intergenic 40.0%
TATTACCAATGATCCTGGTG+GGG - chr8_2:52501796-52501815 None:intergenic 40.0%
TAGAAAACCCTATCTCTCTG+AGG + chr8_2:52502090-52502109 Msa1254360:CDS 40.0%
AGAAAACCCTATCTCTCTGA+GGG + chr8_2:52502091-52502110 Msa1254360:CDS 40.0%
TTAGTTCCCTCAGAGAGATA+GGG - chr8_2:52502100-52502119 None:intergenic 40.0%
! TTTAGTTCCCTCAGAGAGAT+AGG - chr8_2:52502101-52502120 None:intergenic 40.0%
! ACCTTGACTTAGGACTTGTT+TGG + chr8_2:52502217-52502236 Msa1254360:intron 40.0%
TCCAAACAAGTCCTAAGTCA+AGG - chr8_2:52502221-52502240 None:intergenic 40.0%
TAAGCACTTGTGAGACTGTT+TGG + chr8_2:52502256-52502275 Msa1254360:intron 40.0%
AAGCACTTGTGAGACTGTTT+GGG + chr8_2:52502257-52502276 Msa1254360:intron 40.0%
AAGACTGCAGAAATACAAGG+CGG + chr8_2:52502358-52502377 Msa1254360:intron 40.0%
GCCTAACATGCAAAACTGTT+TGG + chr8_2:52502458-52502477 Msa1254360:intron 40.0%
! CCCAAACAGTTTTGCATGTT+AGG - chr8_2:52502462-52502481 None:intergenic 40.0%
CACAAAATTAACGCCCTGTT+TGG - chr8_2:52502696-52502715 None:intergenic 40.0%
!! CACCAAAAAACACTGACTTG+TGG + chr8_2:52502724-52502743 Msa1254360:intron 40.0%
!! AAAACACTGACTTGTGGTTG+TGG + chr8_2:52502730-52502749 Msa1254360:intron 40.0%
! AGCTCCATACTTGTTAGCTA+AGG - chr8_2:52502802-52502821 None:intergenic 40.0%
AGTTATCGATGAACAGCAGA+AGG + chr8_2:52502966-52502985 Msa1254360:intron 40.0%
AACCTTCAGATAAATGCCAG+CGG - chr8_2:52503029-52503048 None:intergenic 40.0%
ACTTAAGAGCCAAGTGTGTT+AGG + chr8_2:52503415-52503434 Msa1254360:intron 40.0%
CTTAAGAGCCAAGTGTGTTA+GGG + chr8_2:52503416-52503435 Msa1254360:intron 40.0%
TTAAGAGCCAAGTGTGTTAG+GGG + chr8_2:52503417-52503436 Msa1254360:intron 40.0%
ATTAGCTCCAACCTACTTTG+CGG - chr8_2:52503448-52503467 None:intergenic 40.0%
! AGTAGGTTGGAGCTAATACT+TGG + chr8_2:52503451-52503470 Msa1254360:intron 40.0%
! GAGCTAATACTTGGAGCTTT+TGG + chr8_2:52503460-52503479 Msa1254360:intron 40.0%
!! TTGGAGCTTTTGGGAAATTG+CGG + chr8_2:52503470-52503489 Msa1254360:intron 40.0%
GAAGCTTAAACATGAGGTAG+AGG + chr8_2:52503499-52503518 Msa1254360:intron 40.0%
! ACATCCTTTTATCGCGTGTT+TGG - chr8_2:52503545-52503564 None:intergenic 40.0%
! ATCGGATATGGAGTCCTTTT+AGG + chr8_2:52503579-52503598 Msa1254360:intron 40.0%
!! GATATGGAGTCCTTTTAGGT+TGG + chr8_2:52503583-52503602 Msa1254360:intron 40.0%
TTCAACGGATCCAACCTAAA+AGG - chr8_2:52503596-52503615 None:intergenic 40.0%
ATGAGAGAGATGGAGTCAAT+GGG - chr8_2:52503707-52503726 None:intergenic 40.0%
AATGAGAGAGATGGAGTCAA+TGG - chr8_2:52503708-52503727 None:intergenic 40.0%
AGCATAGCAGTAAAGGGATT+GGG - chr8_2:52503785-52503804 None:intergenic 40.0%
! ATGGAGAGCATAGCAGTAAA+GGG - chr8_2:52503791-52503810 None:intergenic 40.0%
TGAGCAAATCCAAAACCGAT+AGG + chr8_2:52503864-52503883 Msa1254360:intron 40.0%
!! ATTGATGATGCTGTCAACTG+TGG - chr8_2:52503938-52503957 None:intergenic 40.0%
AGGATTGTGGCATCATTTGA+AGG + chr8_2:52503965-52503984 Msa1254360:intron 40.0%
! TCAGCCACTTTGAAAGAGAA+AGG + chr8_2:52504063-52504082 Msa1254360:intron 40.0%
TGGACTGTGATAAGATTTGG+TGG + chr8_2:52504145-52504164 Msa1254360:intron 40.0%
! GGACTGTGATAAGATTTGGT+GGG + chr8_2:52504146-52504165 Msa1254360:intron 40.0%
ATCCTAGGGCCCATAAAAAA+TGG - chr8_2:52504229-52504248 None:intergenic 40.0%
!! GCCATGCCATTATTTTGTCT+TGG + chr8_2:52504332-52504351 Msa1254360:intron 40.0%
CCCAAGACAAAATAATGGCA+TGG - chr8_2:52504336-52504355 None:intergenic 40.0%
TCAAATGCACTGTCTGTGTT+AGG + chr8_2:52504628-52504647 Msa1254360:intron 40.0%
GGTATATAAGTGTGCGCTTA+AGG + chr8_2:52504744-52504763 Msa1254360:intron 40.0%
AGGACTACAAATTGCTTGAG+CGG + chr8_2:52504843-52504862 Msa1254360:CDS 40.0%
AGCAAGCCAACAGCAATAAT+TGG + chr8_2:52504878-52504897 Msa1254360:CDS 40.0%
ACATCGCCAATTATTGCTGT+TGG - chr8_2:52504887-52504906 None:intergenic 40.0%
! TACAATGGCAAAAGCATGAC+AGG - chr8_2:52505007-52505026 None:intergenic 40.0%
! GTAGTTCGCATTTTGTAGAC+AGG + chr8_2:52505024-52505043 Msa1254360:exon 40.0%
TGCATGACTGTACTTGACTA+AGG + chr8_2:52505136-52505155 Msa1254360:three_prime_UTR 40.0%
GCATGACTAATATTTAGGCC+TGG + chr8_2:52505185-52505204 Msa1254360:three_prime_UTR 40.0%
AATGATGAGTTTGTGAGGAG+TGG + chr8_2:52505214-52505233 Msa1254360:three_prime_UTR 40.0%
CGGTTACTTCTCTGAGTTTG+GGG - chr8_2:52501627-52501646 None:intergenic 45.0%
GCGGTTACTTCTCTGAGTTT+GGG - chr8_2:52501628-52501647 None:intergenic 45.0%
TGCGGTTACTTCTCTGAGTT+TGG - chr8_2:52501629-52501648 None:intergenic 45.0%
!!! GGGTTTTGGAGAGATAAGAG+AGG - chr8_2:52501699-52501718 None:intergenic 45.0%
!! AAGTGGGAGGTGATGGAAAT+GGG - chr8_2:52501743-52501762 None:intergenic 45.0%
!! AAAGTGGGAGGTGATGGAAA+TGG - chr8_2:52501744-52501763 None:intergenic 45.0%
GTTGAAAAAGTGGGAGGTGA+TGG - chr8_2:52501750-52501769 None:intergenic 45.0%
! ACAGCAACTTCAGGTTCTTG+TGG - chr8_2:52502019-52502038 None:intergenic 45.0%
TCAGTAGCTACAGCAACTTC+AGG - chr8_2:52502028-52502047 None:intergenic 45.0%
! TCTCTCTGAGGGAACTAAAC+AGG + chr8_2:52502102-52502121 Msa1254360:CDS 45.0%
GCCAAGACTGCAGAAATACA+AGG + chr8_2:52502355-52502374 Msa1254360:intron 45.0%
! GCCTTGTATTTCTGCAGTCT+TGG - chr8_2:52502359-52502378 None:intergenic 45.0%
GGAGCTGACATTACAGTTGT+AGG + chr8_2:52502816-52502835 Msa1254360:CDS 45.0%
ATCCGCTGGCATTTATCTGA+AGG + chr8_2:52503024-52503043 Msa1254360:CDS 45.0%
!! AGCTTTTGGGAAATTGCGGT+GGG + chr8_2:52503474-52503493 Msa1254360:intron 45.0%
TGGGAGGAAGCTTAAACATG+AGG + chr8_2:52503493-52503512 Msa1254360:intron 45.0%
!! TCAATGGGTTAGATCTGGTG+TGG - chr8_2:52503692-52503711 None:intergenic 45.0%
!! TGGAGTCAATGGGTTAGATC+TGG - chr8_2:52503697-52503716 None:intergenic 45.0%
GAGCATAGCAGTAAAGGGAT+TGG - chr8_2:52503786-52503805 None:intergenic 45.0%
! CATGGAGAGCATAGCAGTAA+AGG - chr8_2:52503792-52503811 None:intergenic 45.0%
! GGTAATGGTAGGCTTCCTAT+CGG - chr8_2:52503882-52503901 None:intergenic 45.0%
!! CATGCTTGCTTGGTAATGGT+AGG - chr8_2:52503893-52503912 None:intergenic 45.0%
!!! GTCCATTTTTTATGGGCCCT+AGG + chr8_2:52504224-52504243 Msa1254360:intron 45.0%
GCATGGCTCAATTACTTGCA+TGG - chr8_2:52504319-52504338 None:intergenic 45.0%
GAGTGAGTGAAGAGCAAAAG+AGG + chr8_2:52504370-52504389 Msa1254360:intron 45.0%
TGAAGAGCAAAAGAGGACGA+AGG + chr8_2:52504377-52504396 Msa1254360:intron 45.0%
!! GAAAAATGCAGGTGGGTTGA+AGG + chr8_2:52504823-52504842 Msa1254360:intron 45.0%
TACAAATTGCTTGAGCGGCT+TGG + chr8_2:52504848-52504867 Msa1254360:CDS 45.0%
! GAGTTTGTGAGGAGTGGATT+TGG + chr8_2:52505220-52505239 Msa1254360:three_prime_UTR 45.0%
! CAATGTGGGCCTCCTATTTT+AGG + chr8_2:52505285-52505304 Msa1254360:three_prime_UTR 45.0%
!!! ATTTATTTTTTCTTTTTAAA+AGG + chr8_2:52504463-52504482 Msa1254360:intron 5.0%
GAAGTAACCGCAAGCATCCA+TGG + chr8_2:52501637-52501656 Msa1254360:exon 50.0%
ATGAACGCCATGGATGCTTG+CGG - chr8_2:52501647-52501666 None:intergenic 50.0%
! GAGGGTTTGACGAGTTGAAG+CGG - chr8_2:52501680-52501699 None:intergenic 50.0%
GGAAGGGGGGTTGAAAAAGT+GGG - chr8_2:52501759-52501778 None:intergenic 50.0%
GCTATCTTGGACAGTCTCAC+AGG - chr8_2:52502548-52502567 None:intergenic 50.0%
GATAGTTGGGTCTCATGCTC+AGG - chr8_2:52503000-52503019 None:intergenic 50.0%
AGATAAATGCCAGCGGATGC+TGG - chr8_2:52503022-52503041 None:intergenic 50.0%
!! GAGCTTTTGGGAAATTGCGG+TGG + chr8_2:52503473-52503492 Msa1254360:intron 50.0%
!! TTTTGGGAAATTGCGGTGGG+AGG + chr8_2:52503477-52503496 Msa1254360:intron 50.0%
! CGCGTGTTTGGTAATCCACT+GGG - chr8_2:52503533-52503552 None:intergenic 50.0%
! TCGCGTGTTTGGTAATCCAC+TGG - chr8_2:52503534-52503553 None:intergenic 50.0%
GAGAGAGGCAATGAGAGAGA+TGG - chr8_2:52503717-52503736 None:intergenic 50.0%
ACTTGCTTCAGCCTTGCACA+TGG - chr8_2:52503826-52503845 None:intergenic 50.0%
!!! GGTAGGCTTCCTATCGGTTT+TGG - chr8_2:52503876-52503895 None:intergenic 50.0%
GATGCTGTCAACTGTGGCTT+TGG - chr8_2:52503932-52503951 None:intergenic 50.0%
GGGCCCTAGGATATGATATC+TGG + chr8_2:52504237-52504256 Msa1254360:intron 50.0%
AGAGCAAAAGAGGACGAAGG+AGG + chr8_2:52504380-52504399 Msa1254360:intron 50.0%
CAAAAGAGGACGAAGGAGGA+AGG + chr8_2:52504384-52504403 Msa1254360:intron 50.0%
AGGAAGGACGAGGGAGATAA+GGG + chr8_2:52504400-52504419 Msa1254360:intron 50.0%
! GGCAAAAGCATGACAGGACA+GGG - chr8_2:52505001-52505020 None:intergenic 50.0%
! TGGCAAAAGCATGACAGGAC+AGG - chr8_2:52505002-52505021 None:intergenic 50.0%
TGGCAGAGTCATGAACGCCA+TGG - chr8_2:52501657-52501676 None:intergenic 55.0%
! GGTTTGACGAGTTGAAGCGG+TGG - chr8_2:52501677-52501696 None:intergenic 55.0%
AGGGGGGTTGAAAAAGTGGG+AGG - chr8_2:52501756-52501775 None:intergenic 55.0%
GGGAAGGGGGGTTGAAAAAG+TGG - chr8_2:52501760-52501779 None:intergenic 55.0%
ACCAATGATCCTGGTGGGGA+GGG - chr8_2:52501792-52501811 None:intergenic 55.0%
TACCAATGATCCTGGTGGGG+AGG - chr8_2:52501793-52501812 None:intergenic 55.0%
CCAGCGGATGCTGGATAGTT+GGG - chr8_2:52503013-52503032 None:intergenic 55.0%
GGTCACACCCCTAACACACT+TGG - chr8_2:52503427-52503446 None:intergenic 55.0%
! TAGGGGTGTGACCGCAAAGT+AGG + chr8_2:52503434-52503453 Msa1254360:intron 55.0%
GGTGTGACCGCAAAGTAGGT+TGG + chr8_2:52503438-52503457 Msa1254360:intron 55.0%
GTAGAGGTGATGTGACCCAG+TGG + chr8_2:52503515-52503534 Msa1254360:intron 55.0%
GCAGTAAAGGGATTGGGAGC+AGG - chr8_2:52503779-52503798 None:intergenic 55.0%
GAGGAAGGACGAGGGAGATA+AGG + chr8_2:52504399-52504418 Msa1254360:intron 55.0%
!! TTGCTTGAGCGGCTTGGTGA+TGG + chr8_2:52504854-52504873 Msa1254360:CDS 55.0%
!! TGCTTGAGCGGCTTGGTGAT+GGG + chr8_2:52504855-52504874 Msa1254360:CDS 55.0%
AGGGTGTGAAGTCGGGAAGG+GGG - chr8_2:52501773-52501792 None:intergenic 60.0%
! GAGGGTGTGAAGTCGGGAAG+GGG - chr8_2:52501774-52501793 None:intergenic 60.0%
! GGAGGGTGTGAAGTCGGGAA+GGG - chr8_2:52501775-52501794 None:intergenic 60.0%
! TGGTGGGGAGGGTGTGAAGT+CGG - chr8_2:52501781-52501800 None:intergenic 60.0%
ACCCTCCCCACCAGGATCAT+TGG + chr8_2:52501788-52501807 Msa1254360:CDS 60.0%
CCCAACTATCCAGCATCCGC+TGG + chr8_2:52503010-52503029 Msa1254360:CDS 60.0%
GCCAGCGGATGCTGGATAGT+TGG - chr8_2:52503014-52503033 None:intergenic 60.0%
AGGACGAAGGAGGAAGGACG+AGG + chr8_2:52504390-52504409 Msa1254360:intron 60.0%
GGACGAAGGAGGAAGGACGA+GGG + chr8_2:52504391-52504410 Msa1254360:intron 60.0%
CGAGGGAGATAAGGGCCACT+CGG + chr8_2:52504408-52504427 Msa1254360:intron 60.0%
GAGGGAGATAAGGGCCACTC+GGG + chr8_2:52504409-52504428 Msa1254360:intron 60.0%
AGGGAGATAAGGGCCACTCG+GGG + chr8_2:52504410-52504429 Msa1254360:intron 60.0%
GGGTGTGAAGTCGGGAAGGG+GGG - chr8_2:52501772-52501791 None:intergenic 65.0%
! GGGAGGGTGTGAAGTCGGGA+AGG - chr8_2:52501776-52501795 None:intergenic 65.0%
! GGTGGGGAGGGTGTGAAGTC+GGG - chr8_2:52501780-52501799 None:intergenic 65.0%
GACTTCACACCCTCCCCACC+AGG + chr8_2:52501780-52501799 Msa1254360:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 52501472 52505463 52501472 ID=Msa1254360;Name=Msa1254360
chr8_2 mRNA 52501472 52505463 52501472 ID=Msa1254360-mRNA-1;Parent=Msa1254360;Name=Msa1254360-mRNA-1;_AED=0.11;_eAED=0.11;_QI=184|1|1|1|1|1|5|436|200
chr8_2 exon 52501472 52501809 52501472 ID=Msa1254360-mRNA-1:exon:16103;Parent=Msa1254360-mRNA-1
chr8_2 exon 52502005 52502123 52502005 ID=Msa1254360-mRNA-1:exon:16104;Parent=Msa1254360-mRNA-1
chr8_2 exon 52502780 52502837 52502780 ID=Msa1254360-mRNA-1:exon:16105;Parent=Msa1254360-mRNA-1
chr8_2 exon 52502968 52503045 52502968 ID=Msa1254360-mRNA-1:exon:16106;Parent=Msa1254360-mRNA-1
chr8_2 exon 52504834 52505463 52504834 ID=Msa1254360-mRNA-1:exon:16107;Parent=Msa1254360-mRNA-1
chr8_2 five_prime_UTR 52501472 52501655 52501472 ID=Msa1254360-mRNA-1:five_prime_utr;Parent=Msa1254360-mRNA-1
chr8_2 CDS 52501656 52501809 52501656 ID=Msa1254360-mRNA-1:cds;Parent=Msa1254360-mRNA-1
chr8_2 CDS 52502005 52502123 52502005 ID=Msa1254360-mRNA-1:cds;Parent=Msa1254360-mRNA-1
chr8_2 CDS 52502780 52502837 52502780 ID=Msa1254360-mRNA-1:cds;Parent=Msa1254360-mRNA-1
chr8_2 CDS 52502968 52503045 52502968 ID=Msa1254360-mRNA-1:cds;Parent=Msa1254360-mRNA-1
chr8_2 CDS 52504834 52505027 52504834 ID=Msa1254360-mRNA-1:cds;Parent=Msa1254360-mRNA-1
chr8_2 three_prime_UTR 52505028 52505463 52505028 ID=Msa1254360-mRNA-1:three_prime_utr;Parent=Msa1254360-mRNA-1
Gene Sequence

>Msa1254360

ATGGCGTTCATGACTCTGCCACCGCTTCAACTCGTCAAACCCTCTCTTATCTCTCCAAAACCCTCCCTATTCTTCAATAACACACCCATTTCCATCACCTCCCACTTTTTCAACCCCCCTTCCCGACTTCACACCCTCCCCACCAGGATCATTGTTAAGGCTAAACCACAAGAACCTGAAGTTGCTGTAGCTACTGATTCATTTACACAATTCAATCATCTTCTTTTGCCTATTACTGATAGAAAACCCTATCTCTCTGAGGGAACTAAACAGGCTATAGCGACTACCATTGCCTTAGCTAACAAGTATGGAGCTGACATTACAGTTGTAGTTATCGATGAACAGCAGAAGGAGTCACTGCCTGAGCATGAGACCCAACTATCCAGCATCCGCTGGCATTTATCTGAAGGTGGGTTGAAGGACTACAAATTGCTTGAGCGGCTTGGTGATGGGAGCAAGCCAACAGCAATAATTGGCGATGTTGCTGATGAACTTAATTTGGATTTGGTAGTTATTAGCATGGAAGCAATTCATACAAAGCATATAGATGCAAATTTGCTTGCTGAGTTCATTCCCTGTCCTGTCATGCTTTTGCCATTGTAG
Protein sequence

>Msa1254360

MAFMTLPPLQLVKPSLISPKPSLFFNNTPISITSHFFNPPSRLHTLPTRIIVKAKPQEPEVAVATDSFTQFNHLLLPITDRKPYLSEGTKQAIATTIALANKYGADITVVVIDEQQKESLPEHETQLSSIRWHLSEGGLKDYKLLERLGDGSKPTAIIGDVADELNLDLVVISMEAIHTKHIDANLLAEFIPCPVMLLPL*