AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1211800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211800 MtrunA17_Chr4g0060581 97.661 171 4 0 5 175 3 173 5.31e-124 346
Msa1211800 MtrunA17_Chr5g0416561 60.221 181 58 4 7 175 59 237 4.43e-75 224
Msa1211800 MtrunA17_Chr5g0443431 50.667 150 72 1 14 161 4 153 1.17e-45 147
Msa1211800 MtrunA17_Chr3g0097141 49.324 148 72 1 17 161 7 154 9.81e-45 145
Msa1211800 MtrunA17_Chr8g0386221 58.163 98 41 0 12 109 2 99 1.18e-35 120
Msa1211800 MtrunA17_Chr3g0132031 56.842 95 41 0 14 108 4 98 2.59e-32 113
Msa1211800 MtrunA17_Chr4g0049701 39.098 133 77 3 24 155 14 143 4.48e-28 106
Msa1211800 MtrunA17_Chr4g0049651 39.041 146 81 2 13 155 6 146 6.59e-26 102
Msa1211800 MtrunA17_Chr4g0049671 40.164 122 65 2 37 155 21 137 2.99e-22 92.8
Msa1211800 MtrunA17_Chr5g0427761 33.684 95 63 0 16 110 11 105 2.46e-16 75.5
Msa1211800 MtrunA17_Chr5g0403731 36.585 82 52 0 34 115 41 122 8.13e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211800 AT1G74520.1 71.264 174 45 1 7 175 2 175 2.90e-91 264
Msa1211800 AT1G74520.2 71.264 174 45 1 7 175 2 175 2.90e-91 264
Msa1211800 AT1G69700.1 56.667 180 68 4 6 175 3 182 3.03e-68 206
Msa1211800 AT5G62490.1 59.412 170 60 4 8 175 3 165 3.58e-64 195
Msa1211800 AT2G42820.1 42.105 152 85 2 13 161 3 154 5.27e-40 133
Msa1211800 AT5G50720.1 60.440 91 36 0 18 108 8 98 1.37e-32 113
Msa1211800 AT4G24960.1 54.762 84 38 0 25 108 15 98 2.26e-29 105
Msa1211800 AT4G24960.3 55.128 78 35 0 31 108 2 79 3.77e-26 97.4
Msa1211800 AT4G24960.2 50.746 67 33 0 42 108 1 67 2.51e-20 81.3
Msa1211800 AT4G36720.1 35.227 88 57 0 29 116 44 131 1.66e-14 68.6

Find 69 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATACCATTCAAGAATTT+TGG 0.138822 8_1:+71522869 None:intergenic
TAAACACTCTTGTAAGTATT+TGG 0.202218 8_1:-71521511 Msa1211800:three_prime_UTR
TGCAGGCTGATAAATCTAAA+TGG 0.232888 8_1:-71521908 Msa1211800:intron
AAGCCATCGTTATTAAAGTA+TGG 0.240142 8_1:-71521593 Msa1211800:three_prime_UTR
ACGACACAACAGAGATGTTT+TGG 0.266829 8_1:+71523402 None:intergenic
TTTCCAAGGAGAGATATTTA+AGG 0.267467 8_1:-71521768 Msa1211800:three_prime_UTR
GCATTATGTAAGACCCTTCT+TGG 0.297262 8_1:-71522144 Msa1211800:CDS
ATGCTTCTGTTCCATTCTCT+TGG 0.314598 8_1:+71522027 None:intergenic
AACTTTGCATATGGCCATAT+AGG 0.318540 8_1:+71522224 None:intergenic
GCTTCTGCCTCTTGTGGATA+AGG 0.336708 8_1:-71521833 Msa1211800:three_prime_UTR
ATCAACAGTGGCTAACCTAT+TGG 0.338612 8_1:-71522932 Msa1211800:CDS
TATTAAAGTATGGGTGTGTT+TGG 0.346356 8_1:-71521583 Msa1211800:three_prime_UTR
CTTCTGCCTCTTGTGGATAA+GGG 0.363725 8_1:-71521832 Msa1211800:three_prime_UTR
TATATTTCAGGATCCCTATA+TGG 0.368282 8_1:-71522238 Msa1211800:intron
TAAGCAGCACCAGTAAAATA+AGG 0.374751 8_1:+71522176 None:intergenic
GATCAAAAGATGGGTGGATC+TGG 0.375142 8_1:-71523328 Msa1211800:exon
GGTGGATCTGGATCTGGATC+TGG 0.381308 8_1:-71523316 Msa1211800:CDS
ACTTTGCATATGGCCATATA+GGG 0.384994 8_1:+71522225 None:intergenic
GCAGGCTGATAAATCTAAAT+GGG 0.400706 8_1:-71521907 Msa1211800:intron
TGGAACATACCAGATGTTAA+TGG 0.405359 8_1:+71522109 None:intergenic
GTTAGAATAAACTTTGCATA+TGG 0.423777 8_1:+71522215 None:intergenic
TAGTGTGAGTTGTACTTGTT+GGG 0.423933 8_1:+71523450 None:intergenic
TCAACAGTGGCTAACCTATT+GGG 0.425769 8_1:-71522931 Msa1211800:CDS
CTGGTATGTTCCAAGGAAAA+AGG 0.426307 8_1:-71522099 Msa1211800:CDS
CTCCAGATATGCTTCGGTTA+GGG 0.429295 8_1:-71522982 Msa1211800:intron
AGGTCTCATCATACATTGTA+TGG 0.438227 8_1:+71521874 None:intergenic
GCTACAAGATTGCATTCAAT+AGG 0.452212 8_1:+71521854 None:intergenic
GTAGTGTGAGTTGTACTTGT+TGG 0.458679 8_1:+71523449 None:intergenic
GCAGCAGTTATAATGTCATC+TGG 0.464513 8_1:+71522062 None:intergenic
CAAAGTTTATTCTAACCTGC+TGG 0.465022 8_1:-71522208 Msa1211800:CDS
TGTATTTCTAAATTTGTATG+TGG 0.467627 8_1:+71523359 None:intergenic
CAATGAATACAGAACCCAAT+AGG 0.470528 8_1:+71522917 None:intergenic
ACTCCAGATATGCTTCGGTT+AGG 0.470884 8_1:-71522983 Msa1211800:intron
AAACCTTAAATATCTCTCCT+TGG 0.492023 8_1:+71521765 None:intergenic
CTTGTAGCTTCTGCCTCTTG+TGG 0.495195 8_1:-71521839 Msa1211800:three_prime_UTR
AAGATGGGTGGATCTGGATC+TGG 0.496666 8_1:-71523322 Msa1211800:CDS
GATGTTAATGGTCTGAGGAT+TGG 0.500959 8_1:+71522121 None:intergenic
ATCCTCAGACCATTAACATC+TGG 0.502606 8_1:-71522118 Msa1211800:CDS
ACTGTACTCCAGATATGCTT+CGG 0.503546 8_1:-71522988 Msa1211800:intron
TTCTGCCTCTTGTGGATAAG+GGG 0.505223 8_1:-71521831 Msa1211800:three_prime_UTR
GAGAAGTACATCCAAGAGAA+TGG 0.508499 8_1:-71522038 Msa1211800:CDS
CATTTGAGAATCTGATCCAT+AGG 0.534447 8_1:-71522007 Msa1211800:CDS
CATAACACAAGATCAAAAGA+TGG 0.537513 8_1:-71523338 Msa1211800:exon
GGTCTGAGGATTGGCCAAGA+AGG 0.538778 8_1:+71522130 None:intergenic
CACCCATACTTTAATAACGA+TGG 0.546460 8_1:+71521590 None:intergenic
AGCCATCGTTATTAAAGTAT+GGG 0.547951 8_1:-71521592 Msa1211800:three_prime_UTR
TCTGGATCTGGATCTGGTGC+CGG 0.555793 8_1:-71523310 Msa1211800:CDS
GTCTGAGGATTGGCCAAGAA+GGG 0.556969 8_1:+71522131 None:intergenic
ATAAGGAAGGACCAACCAGC+AGG 0.566345 8_1:+71522193 None:intergenic
GTTTATTCTAACCTGCTGGT+TGG 0.569022 8_1:-71522204 Msa1211800:CDS
GGGCTTGACTATCGAGTGAA+AGG 0.570616 8_1:+71523470 None:intergenic
TAGCCACTGTTGATCATCCA+CGG 0.583333 8_1:+71522941 None:intergenic
TTAACATCTGGTATGTTCCA+AGG 0.592131 8_1:-71522106 Msa1211800:CDS
ATTTGAGAATCTGATCCATA+GGG 0.594382 8_1:-71522006 Msa1211800:intron
AGCCACTGTTGATCATCCAC+GGG 0.594983 8_1:+71522942 None:intergenic
ATAACACAAGATCAAAAGAT+GGG 0.599902 8_1:-71523337 Msa1211800:exon
ATATGATATATAGATGATGT+TGG 0.606486 8_1:-71521661 Msa1211800:three_prime_UTR
TCTGCCTCTTGTGGATAAGG+GGG 0.607999 8_1:-71521830 Msa1211800:three_prime_UTR
ATATGTGTGTTTGTGTGCAG+TGG 0.613365 8_1:+71523539 None:intergenic
AAGAACCATGAGAAAACTAC+CGG 0.617636 8_1:+71523291 None:intergenic
TACCAGATGTTAATGGTCTG+AGG 0.621335 8_1:+71522116 None:intergenic
CAGCACCAGTAAAATAAGGA+AGG 0.622937 8_1:+71522180 None:intergenic
ACACAAGATCAAAAGATGGG+TGG 0.630784 8_1:-71523334 Msa1211800:exon
GGATAAACAAGACTAATCAC+AGG 0.636906 8_1:+71523115 None:intergenic
AATTGAGAGCAAGTCTCCCG+TGG 0.662927 8_1:-71522958 Msa1211800:CDS
AGATGATGTTGGTACAACAG+TGG 0.671646 8_1:-71521650 Msa1211800:three_prime_UTR
GCTGATAAATCTAAATGGGG+CGG 0.685381 8_1:-71521903 Msa1211800:CDS
CAGGCTGATAAATCTAAATG+GGG 0.691520 8_1:-71521906 Msa1211800:CDS
CTCCCGTGGATGATCAACAG+TGG 0.706862 8_1:-71522944 Msa1211800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAATGAATTTTCTTT+TGG - chr8_1:71522307-71522326 Msa1211800:intron 10.0%
!!! TTTTTAATGAATTTTCTTTT+GGG - chr8_1:71522308-71522327 Msa1211800:intron 10.0%
!! AATAATGATTGATAAATATT+TGG + chr8_1:71522419-71522438 None:intergenic 10.0%
!! AATATACTTTATAATTTAAC+TGG - chr8_1:71522634-71522653 Msa1211800:intron 10.0%
!!! TTTTTTAATGAATTTTCTTT+TGG - chr8_1:71522307-71522326 Msa1211800:intron 10.0%
!!! TTTTTAATGAATTTTCTTTT+GGG - chr8_1:71522308-71522327 Msa1211800:intron 10.0%
!! AATAATGATTGATAAATATT+TGG + chr8_1:71522419-71522438 None:intergenic 10.0%
!! AATATACTTTATAATTTAAC+TGG - chr8_1:71522634-71522653 Msa1211800:intron 10.0%
!! AAAAAAAAAATCTTAAGAAG+AGG + chr8_1:71521806-71521825 None:intergenic 15.0%
!! ATAAGTGAAAATCATAAAAA+GGG + chr8_1:71522166-71522185 None:intergenic 15.0%
!! AATAAGTGAAAATCATAAAA+AGG + chr8_1:71522167-71522186 None:intergenic 15.0%
!!! ATTTTCACAATATATATTTC+AGG - chr8_1:71522757-71522776 Msa1211800:intron 15.0%
!! AAAAAAAAAATCTTAAGAAG+AGG + chr8_1:71521806-71521825 None:intergenic 15.0%
!! ATAAGTGAAAATCATAAAAA+GGG + chr8_1:71522166-71522185 None:intergenic 15.0%
!! AATAAGTGAAAATCATAAAA+AGG + chr8_1:71522167-71522186 None:intergenic 15.0%
!!! ATTTTCACAATATATATTTC+AGG - chr8_1:71522757-71522776 Msa1211800:intron 15.0%
!!! TGTATTTCTAAATTTGTATG+TGG + chr8_1:71521651-71521670 None:intergenic 20.0%
!! ACTAATTGTCACATATTAAA+GGG + chr8_1:71521939-71521958 None:intergenic 20.0%
!!! TTTATGATTTTCACTTATTC+AGG - chr8_1:71522168-71522187 Msa1211800:CDS 20.0%
!!! TTATGATTTTCACTTATTCA+GGG - chr8_1:71522169-71522188 Msa1211800:CDS 20.0%
!! ACAATATTAGTAAATCACAA+CGG + chr8_1:71522587-71522606 None:intergenic 20.0%
!!! TTTTTATGAGTATGTTTAGA+AGG - chr8_1:71522706-71522725 Msa1211800:intron 20.0%
!!! TTTTATGAGTATGTTTAGAA+GGG - chr8_1:71522707-71522726 Msa1211800:intron 20.0%
!!! TATTTAGTTTATGTTTTCCA+AGG - chr8_1:71523225-71523244 Msa1211800:intron 20.0%
!! GTTACACAACAAAAAATAAA+GGG + chr8_1:71523274-71523293 None:intergenic 20.0%
!! AGTTACACAACAAAAAATAA+AGG + chr8_1:71523275-71523294 None:intergenic 20.0%
!! ATATGATATATAGATGATGT+TGG - chr8_1:71523346-71523365 Msa1211800:five_prime_UTR 20.0%
!!! TGTATTTCTAAATTTGTATG+TGG + chr8_1:71521651-71521670 None:intergenic 20.0%
!! ACTAATTGTCACATATTAAA+GGG + chr8_1:71521939-71521958 None:intergenic 20.0%
!!! TTTATGATTTTCACTTATTC+AGG - chr8_1:71522168-71522187 Msa1211800:CDS 20.0%
!!! TTATGATTTTCACTTATTCA+GGG - chr8_1:71522169-71522188 Msa1211800:CDS 20.0%
!! ACAATATTAGTAAATCACAA+CGG + chr8_1:71522587-71522606 None:intergenic 20.0%
!!! TTTTTATGAGTATGTTTAGA+AGG - chr8_1:71522706-71522725 Msa1211800:intron 20.0%
!!! TTTTATGAGTATGTTTAGAA+GGG - chr8_1:71522707-71522726 Msa1211800:intron 20.0%
!!! TATTTAGTTTATGTTTTCCA+AGG - chr8_1:71523225-71523244 Msa1211800:intron 20.0%
!! GTTACACAACAAAAAATAAA+GGG + chr8_1:71523274-71523293 None:intergenic 20.0%
!! AGTTACACAACAAAAAATAA+AGG + chr8_1:71523275-71523294 None:intergenic 20.0%
!! ATATGATATATAGATGATGT+TGG - chr8_1:71523346-71523365 Msa1211800:five_prime_UTR 20.0%
! GAAAGGAATAAGAAAAAAAG+TGG + chr8_1:71521523-71521542 None:intergenic 25.0%
! ATAACACAAGATCAAAAGAT+GGG - chr8_1:71521670-71521689 Msa1211800:three_prime_UTR 25.0%
! CACTAATTGTCACATATTAA+AGG + chr8_1:71521940-71521959 None:intergenic 25.0%
! TGGAATGATTTATAGTTATG+TGG - chr8_1:71521988-71522007 Msa1211800:intron 25.0%
! GGAATGATTTATAGTTATGT+GGG - chr8_1:71521989-71522008 Msa1211800:intron 25.0%
!! GTTTAAATTTTACGCTTTGT+TGG - chr8_1:71522222-71522241 Msa1211800:CDS 25.0%
! ATCTTAAGAAAATGTGGTTA+AGG - chr8_1:71522277-71522296 Msa1211800:intron 25.0%
!! TAACTTTTGTATATCTGCAT+TGG - chr8_1:71522455-71522474 Msa1211800:intron 25.0%
! GTTAGAATAAACTTTGCATA+TGG + chr8_1:71522795-71522814 None:intergenic 25.0%
!!! TTTTGTTGTGTAACTATCAT+AGG - chr8_1:71523280-71523299 Msa1211800:CDS 25.0%
! TAAACACTCTTGTAAGTATT+TGG - chr8_1:71523496-71523515 Msa1211800:five_prime_UTR 25.0%
! GAAAGGAATAAGAAAAAAAG+TGG + chr8_1:71521523-71521542 None:intergenic 25.0%
! ATAACACAAGATCAAAAGAT+GGG - chr8_1:71521670-71521689 Msa1211800:three_prime_UTR 25.0%
! CACTAATTGTCACATATTAA+AGG + chr8_1:71521940-71521959 None:intergenic 25.0%
! TGGAATGATTTATAGTTATG+TGG - chr8_1:71521988-71522007 Msa1211800:intron 25.0%
! GGAATGATTTATAGTTATGT+GGG - chr8_1:71521989-71522008 Msa1211800:intron 25.0%
!! GTTTAAATTTTACGCTTTGT+TGG - chr8_1:71522222-71522241 Msa1211800:CDS 25.0%
! ATCTTAAGAAAATGTGGTTA+AGG - chr8_1:71522277-71522296 Msa1211800:intron 25.0%
!! TAACTTTTGTATATCTGCAT+TGG - chr8_1:71522455-71522474 Msa1211800:intron 25.0%
! GTTAGAATAAACTTTGCATA+TGG + chr8_1:71522795-71522814 None:intergenic 25.0%
!!! TTTTGTTGTGTAACTATCAT+AGG - chr8_1:71523280-71523299 Msa1211800:CDS 25.0%
! TAAACACTCTTGTAAGTATT+TGG - chr8_1:71523496-71523515 Msa1211800:five_prime_UTR 25.0%
CATAACACAAGATCAAAAGA+TGG - chr8_1:71521669-71521688 Msa1211800:three_prime_UTR 30.0%
!! AGAAATTTTGATGTACTTGC+TGG - chr8_1:71521742-71521761 Msa1211800:three_prime_UTR 30.0%
!! GAAATTTTGATGTACTTGCT+GGG - chr8_1:71521743-71521762 Msa1211800:three_prime_UTR 30.0%
ATCTTAAGAAGAGGAAAGTA+AGG + chr8_1:71521797-71521816 None:intergenic 30.0%
! CAAGTTAAAAAGAAGCACAT+TGG + chr8_1:71521842-71521861 None:intergenic 30.0%
TCATTGTTTAGTGATGAGAA+TGG - chr8_1:71521968-71521987 Msa1211800:intron 30.0%
! CTTTTGCCAAAATTCTTGAA+TGG - chr8_1:71522132-71522151 Msa1211800:CDS 30.0%
! GGTATACCATTCAAGAATTT+TGG + chr8_1:71522141-71522160 None:intergenic 30.0%
GTGAAAATCATAAAAAGGGT+TGG + chr8_1:71522162-71522181 None:intergenic 30.0%
GACATCATCTTAAGAAAATG+TGG - chr8_1:71522271-71522290 Msa1211800:intron 30.0%
!! TGAATTTTCTTTTGGGAAGT+TGG - chr8_1:71522315-71522334 Msa1211800:intron 30.0%
!! GAATTTTCTTTTGGGAAGTT+GGG - chr8_1:71522316-71522335 Msa1211800:intron 30.0%
ATTAGTAAATCACAACGGAA+AGG + chr8_1:71522582-71522601 None:intergenic 30.0%
TATATTTCAGGATCCCTATA+TGG - chr8_1:71522769-71522788 Msa1211800:intron 30.0%
ATTTGAGAATCTGATCCATA+GGG - chr8_1:71523001-71523020 Msa1211800:intron 30.0%
TAATAACCAAGTGTTACAGA+TGG + chr8_1:71523032-71523051 None:intergenic 30.0%
TTTCCAAGGAGAGATATTTA+AGG - chr8_1:71523239-71523258 Msa1211800:intron 30.0%
AAACCTTAAATATCTCTCCT+TGG + chr8_1:71523245-71523264 None:intergenic 30.0%
! TTGATTTTCTCGTGTTTTAC+TGG - chr8_1:71523387-71523406 Msa1211800:five_prime_UTR 30.0%
AAGCCATCGTTATTAAAGTA+TGG - chr8_1:71523414-71523433 Msa1211800:five_prime_UTR 30.0%
AGCCATCGTTATTAAAGTAT+GGG - chr8_1:71523415-71523434 Msa1211800:five_prime_UTR 30.0%
TATTAAAGTATGGGTGTGTT+TGG - chr8_1:71523424-71523443 Msa1211800:five_prime_UTR 30.0%
CATAACACAAGATCAAAAGA+TGG - chr8_1:71521669-71521688 Msa1211800:three_prime_UTR 30.0%
!! AGAAATTTTGATGTACTTGC+TGG - chr8_1:71521742-71521761 Msa1211800:three_prime_UTR 30.0%
!! GAAATTTTGATGTACTTGCT+GGG - chr8_1:71521743-71521762 Msa1211800:three_prime_UTR 30.0%
ATCTTAAGAAGAGGAAAGTA+AGG + chr8_1:71521797-71521816 None:intergenic 30.0%
! CAAGTTAAAAAGAAGCACAT+TGG + chr8_1:71521842-71521861 None:intergenic 30.0%
TCATTGTTTAGTGATGAGAA+TGG - chr8_1:71521968-71521987 Msa1211800:intron 30.0%
! CTTTTGCCAAAATTCTTGAA+TGG - chr8_1:71522132-71522151 Msa1211800:CDS 30.0%
! GGTATACCATTCAAGAATTT+TGG + chr8_1:71522141-71522160 None:intergenic 30.0%
GTGAAAATCATAAAAAGGGT+TGG + chr8_1:71522162-71522181 None:intergenic 30.0%
GACATCATCTTAAGAAAATG+TGG - chr8_1:71522271-71522290 Msa1211800:intron 30.0%
!! TGAATTTTCTTTTGGGAAGT+TGG - chr8_1:71522315-71522334 Msa1211800:intron 30.0%
!! GAATTTTCTTTTGGGAAGTT+GGG - chr8_1:71522316-71522335 Msa1211800:intron 30.0%
ATTAGTAAATCACAACGGAA+AGG + chr8_1:71522582-71522601 None:intergenic 30.0%
TATATTTCAGGATCCCTATA+TGG - chr8_1:71522769-71522788 Msa1211800:intron 30.0%
ATTTGAGAATCTGATCCATA+GGG - chr8_1:71523001-71523020 Msa1211800:intron 30.0%
TAATAACCAAGTGTTACAGA+TGG + chr8_1:71523032-71523051 None:intergenic 30.0%
TTTCCAAGGAGAGATATTTA+AGG - chr8_1:71523239-71523258 Msa1211800:intron 30.0%
AAACCTTAAATATCTCTCCT+TGG + chr8_1:71523245-71523264 None:intergenic 30.0%
! TTGATTTTCTCGTGTTTTAC+TGG - chr8_1:71523387-71523406 Msa1211800:five_prime_UTR 30.0%
AAGCCATCGTTATTAAAGTA+TGG - chr8_1:71523414-71523433 Msa1211800:five_prime_UTR 30.0%
AGCCATCGTTATTAAAGTAT+GGG - chr8_1:71523415-71523434 Msa1211800:five_prime_UTR 30.0%
TATTAAAGTATGGGTGTGTT+TGG - chr8_1:71523424-71523443 Msa1211800:five_prime_UTR 30.0%
!! GAAAAAAAGTGGACTGTTGT+TGG + chr8_1:71521512-71521531 None:intergenic 35.0%
TAGTGTGAGTTGTACTTGTT+GGG + chr8_1:71521560-71521579 None:intergenic 35.0%
AAGAACCATGAGAAAACTAC+CGG + chr8_1:71521719-71521738 None:intergenic 35.0%
GGATAAACAAGACTAATCAC+AGG + chr8_1:71521895-71521914 None:intergenic 35.0%
! AATAGAGAGTGTGCTTACAA+AGG + chr8_1:71521916-71521935 None:intergenic 35.0%
CAATGAATACAGAACCCAAT+AGG + chr8_1:71522093-71522112 None:intergenic 35.0%
! TTGTTGGTTGTATCATGTTG+TGG - chr8_1:71522238-71522257 Msa1211800:intron 35.0%
!! CTGGGAAAGCATTAAACTTT+CGG - chr8_1:71522730-71522749 Msa1211800:intron 35.0%
ACTTTGCATATGGCCATATA+GGG + chr8_1:71522785-71522804 None:intergenic 35.0%
AACTTTGCATATGGCCATAT+AGG + chr8_1:71522786-71522805 None:intergenic 35.0%
CAAAGTTTATTCTAACCTGC+TGG - chr8_1:71522799-71522818 Msa1211800:intron 35.0%
!!! TTGGTCCTTCCTTATTTTAC+TGG - chr8_1:71522822-71522841 Msa1211800:intron 35.0%
TAAGCAGCACCAGTAAAATA+AGG + chr8_1:71522834-71522853 None:intergenic 35.0%
TGGAACATACCAGATGTTAA+TGG + chr8_1:71522901-71522920 None:intergenic 35.0%
TTAACATCTGGTATGTTCCA+AGG - chr8_1:71522901-71522920 Msa1211800:CDS 35.0%
CATTTGAGAATCTGATCCAT+AGG - chr8_1:71523000-71523019 Msa1211800:intron 35.0%
TTACAGATGGAAATACCCTA+TGG + chr8_1:71523019-71523038 None:intergenic 35.0%
GTATTTCCATCTGTAACACT+TGG - chr8_1:71523023-71523042 Msa1211800:intron 35.0%
TGCAGGCTGATAAATCTAAA+TGG - chr8_1:71523099-71523118 Msa1211800:intron 35.0%
GCAGGCTGATAAATCTAAAT+GGG - chr8_1:71523100-71523119 Msa1211800:intron 35.0%
CAGGCTGATAAATCTAAATG+GGG - chr8_1:71523101-71523120 Msa1211800:intron 35.0%
AGGTCTCATCATACATTGTA+TGG + chr8_1:71523136-71523155 None:intergenic 35.0%
GCTACAAGATTGCATTCAAT+AGG + chr8_1:71523156-71523175 None:intergenic 35.0%
CACCCATACTTTAATAACGA+TGG + chr8_1:71523420-71523439 None:intergenic 35.0%
!! GAAAAAAAGTGGACTGTTGT+TGG + chr8_1:71521512-71521531 None:intergenic 35.0%
TAGTGTGAGTTGTACTTGTT+GGG + chr8_1:71521560-71521579 None:intergenic 35.0%
AAGAACCATGAGAAAACTAC+CGG + chr8_1:71521719-71521738 None:intergenic 35.0%
GGATAAACAAGACTAATCAC+AGG + chr8_1:71521895-71521914 None:intergenic 35.0%
! AATAGAGAGTGTGCTTACAA+AGG + chr8_1:71521916-71521935 None:intergenic 35.0%
CAATGAATACAGAACCCAAT+AGG + chr8_1:71522093-71522112 None:intergenic 35.0%
! TTGTTGGTTGTATCATGTTG+TGG - chr8_1:71522238-71522257 Msa1211800:intron 35.0%
!! CTGGGAAAGCATTAAACTTT+CGG - chr8_1:71522730-71522749 Msa1211800:intron 35.0%
ACTTTGCATATGGCCATATA+GGG + chr8_1:71522785-71522804 None:intergenic 35.0%
AACTTTGCATATGGCCATAT+AGG + chr8_1:71522786-71522805 None:intergenic 35.0%
CAAAGTTTATTCTAACCTGC+TGG - chr8_1:71522799-71522818 Msa1211800:intron 35.0%
!!! TTGGTCCTTCCTTATTTTAC+TGG - chr8_1:71522822-71522841 Msa1211800:intron 35.0%
TAAGCAGCACCAGTAAAATA+AGG + chr8_1:71522834-71522853 None:intergenic 35.0%
TGGAACATACCAGATGTTAA+TGG + chr8_1:71522901-71522920 None:intergenic 35.0%
TTAACATCTGGTATGTTCCA+AGG - chr8_1:71522901-71522920 Msa1211800:CDS 35.0%
CATTTGAGAATCTGATCCAT+AGG - chr8_1:71523000-71523019 Msa1211800:intron 35.0%
TTACAGATGGAAATACCCTA+TGG + chr8_1:71523019-71523038 None:intergenic 35.0%
GTATTTCCATCTGTAACACT+TGG - chr8_1:71523023-71523042 Msa1211800:intron 35.0%
TGCAGGCTGATAAATCTAAA+TGG - chr8_1:71523099-71523118 Msa1211800:intron 35.0%
GCAGGCTGATAAATCTAAAT+GGG - chr8_1:71523100-71523119 Msa1211800:intron 35.0%
CAGGCTGATAAATCTAAATG+GGG - chr8_1:71523101-71523120 Msa1211800:intron 35.0%
AGGTCTCATCATACATTGTA+TGG + chr8_1:71523136-71523155 None:intergenic 35.0%
GCTACAAGATTGCATTCAAT+AGG + chr8_1:71523156-71523175 None:intergenic 35.0%
CACCCATACTTTAATAACGA+TGG + chr8_1:71523420-71523439 None:intergenic 35.0%
GTAGTGTGAGTTGTACTTGT+TGG + chr8_1:71521561-71521580 None:intergenic 40.0%
! CGACACAACAGAGATGTTTT+GGG + chr8_1:71521607-71521626 None:intergenic 40.0%
! ACGACACAACAGAGATGTTT+TGG + chr8_1:71521608-71521627 None:intergenic 40.0%
ACACAAGATCAAAAGATGGG+TGG - chr8_1:71521673-71521692 Msa1211800:three_prime_UTR 40.0%
GAGGAAAGTAAGGCACATTA+GGG + chr8_1:71521787-71521806 None:intergenic 40.0%
AGAGGAAAGTAAGGCACATT+AGG + chr8_1:71521788-71521807 None:intergenic 40.0%
! AGAAGCACATTGGTCTCAAT+TGG + chr8_1:71521832-71521851 None:intergenic 40.0%
!! GTTGTCGTTGTTTCTATTGC+AGG - chr8_1:71521869-71521888 Msa1211800:CDS 40.0%
ACTGTACTCCAGATATGCTT+CGG - chr8_1:71522019-71522038 Msa1211800:CDS 40.0%
ATCAACAGTGGCTAACCTAT+TGG - chr8_1:71522075-71522094 Msa1211800:CDS 40.0%
TCAACAGTGGCTAACCTATT+GGG - chr8_1:71522076-71522095 Msa1211800:CDS 40.0%
ACTAGAATAACCCTGCAGAT+TGG + chr8_1:71522378-71522397 None:intergenic 40.0%
TCACTACAAGCACATCCAAA+GGG + chr8_1:71522481-71522500 None:intergenic 40.0%
CCTAAACGAAACATGCATTC+AGG + chr8_1:71522514-71522533 None:intergenic 40.0%
CCTGAATGCATGTTTCGTTT+AGG - chr8_1:71522511-71522530 Msa1211800:intron 40.0%
ATGAGTATGTTTAGAAGGGC+TGG - chr8_1:71522711-71522730 Msa1211800:intron 40.0%
TGAGTATGTTTAGAAGGGCT+GGG - chr8_1:71522712-71522731 Msa1211800:intron 40.0%
GTTTATTCTAACCTGCTGGT+TGG - chr8_1:71522803-71522822 Msa1211800:intron 40.0%
CAGCACCAGTAAAATAAGGA+AGG + chr8_1:71522830-71522849 None:intergenic 40.0%
GCATTATGTAAGACCCTTCT+TGG - chr8_1:71522863-71522882 Msa1211800:intron 40.0%
GATGTTAATGGTCTGAGGAT+TGG + chr8_1:71522889-71522908 None:intergenic 40.0%
ATCCTCAGACCATTAACATC+TGG - chr8_1:71522889-71522908 Msa1211800:CDS 40.0%
TACCAGATGTTAATGGTCTG+AGG + chr8_1:71522894-71522913 None:intergenic 40.0%
CTGGTATGTTCCAAGGAAAA+AGG - chr8_1:71522908-71522927 Msa1211800:CDS 40.0%
GCAGCAGTTATAATGTCATC+TGG + chr8_1:71522948-71522967 None:intergenic 40.0%
! GAGAAGTACATCCAAGAGAA+TGG - chr8_1:71522969-71522988 Msa1211800:CDS 40.0%
ATGCTTCTGTTCCATTCTCT+TGG + chr8_1:71522983-71523002 None:intergenic 40.0%
GCTGATAAATCTAAATGGGG+CGG - chr8_1:71523104-71523123 Msa1211800:intron 40.0%
AGATGATGTTGGTACAACAG+TGG - chr8_1:71523357-71523376 Msa1211800:five_prime_UTR 40.0%
GTAGTGTGAGTTGTACTTGT+TGG + chr8_1:71521561-71521580 None:intergenic 40.0%
! CGACACAACAGAGATGTTTT+GGG + chr8_1:71521607-71521626 None:intergenic 40.0%
! ACGACACAACAGAGATGTTT+TGG + chr8_1:71521608-71521627 None:intergenic 40.0%
ACACAAGATCAAAAGATGGG+TGG - chr8_1:71521673-71521692 Msa1211800:three_prime_UTR 40.0%
GAGGAAAGTAAGGCACATTA+GGG + chr8_1:71521787-71521806 None:intergenic 40.0%
AGAGGAAAGTAAGGCACATT+AGG + chr8_1:71521788-71521807 None:intergenic 40.0%
! AGAAGCACATTGGTCTCAAT+TGG + chr8_1:71521832-71521851 None:intergenic 40.0%
!! GTTGTCGTTGTTTCTATTGC+AGG - chr8_1:71521869-71521888 Msa1211800:CDS 40.0%
ACTGTACTCCAGATATGCTT+CGG - chr8_1:71522019-71522038 Msa1211800:CDS 40.0%
ATCAACAGTGGCTAACCTAT+TGG - chr8_1:71522075-71522094 Msa1211800:CDS 40.0%
TCAACAGTGGCTAACCTATT+GGG - chr8_1:71522076-71522095 Msa1211800:CDS 40.0%
ACTAGAATAACCCTGCAGAT+TGG + chr8_1:71522378-71522397 None:intergenic 40.0%
TCACTACAAGCACATCCAAA+GGG + chr8_1:71522481-71522500 None:intergenic 40.0%
CCTAAACGAAACATGCATTC+AGG + chr8_1:71522514-71522533 None:intergenic 40.0%
CCTGAATGCATGTTTCGTTT+AGG - chr8_1:71522511-71522530 Msa1211800:intron 40.0%
ATGAGTATGTTTAGAAGGGC+TGG - chr8_1:71522711-71522730 Msa1211800:intron 40.0%
TGAGTATGTTTAGAAGGGCT+GGG - chr8_1:71522712-71522731 Msa1211800:intron 40.0%
GTTTATTCTAACCTGCTGGT+TGG - chr8_1:71522803-71522822 Msa1211800:intron 40.0%
CAGCACCAGTAAAATAAGGA+AGG + chr8_1:71522830-71522849 None:intergenic 40.0%
GCATTATGTAAGACCCTTCT+TGG - chr8_1:71522863-71522882 Msa1211800:intron 40.0%
GATGTTAATGGTCTGAGGAT+TGG + chr8_1:71522889-71522908 None:intergenic 40.0%
ATCCTCAGACCATTAACATC+TGG - chr8_1:71522889-71522908 Msa1211800:CDS 40.0%
TACCAGATGTTAATGGTCTG+AGG + chr8_1:71522894-71522913 None:intergenic 40.0%
CTGGTATGTTCCAAGGAAAA+AGG - chr8_1:71522908-71522927 Msa1211800:CDS 40.0%
GCAGCAGTTATAATGTCATC+TGG + chr8_1:71522948-71522967 None:intergenic 40.0%
! GAGAAGTACATCCAAGAGAA+TGG - chr8_1:71522969-71522988 Msa1211800:CDS 40.0%
ATGCTTCTGTTCCATTCTCT+TGG + chr8_1:71522983-71523002 None:intergenic 40.0%
GCTGATAAATCTAAATGGGG+CGG - chr8_1:71523104-71523123 Msa1211800:intron 40.0%
AGATGATGTTGGTACAACAG+TGG - chr8_1:71523357-71523376 Msa1211800:five_prime_UTR 40.0%
GATCAAAAGATGGGTGGATC+TGG - chr8_1:71521679-71521698 Msa1211800:three_prime_UTR 45.0%
ACTCCAGATATGCTTCGGTT+AGG - chr8_1:71522024-71522043 Msa1211800:CDS 45.0%
CTCCAGATATGCTTCGGTTA+GGG - chr8_1:71522025-71522044 Msa1211800:CDS 45.0%
TAGCCACTGTTGATCATCCA+CGG + chr8_1:71522069-71522088 None:intergenic 45.0%
TATCATCTGCGTGTGTAAGC+TGG - chr8_1:71522339-71522358 Msa1211800:intron 45.0%
! GTATATCTGCATTGGCCCTT+TGG - chr8_1:71522463-71522482 Msa1211800:intron 45.0%
GTCACTACAAGCACATCCAA+AGG + chr8_1:71522482-71522501 None:intergenic 45.0%
! GTGAAGACGTCCTTTTTCCT+TGG + chr8_1:71522921-71522940 None:intergenic 45.0%
TTTATCAGCCTGCAGAGTGA+TGG + chr8_1:71523093-71523112 None:intergenic 45.0%
CTTCTGCCTCTTGTGGATAA+GGG - chr8_1:71523175-71523194 Msa1211800:intron 45.0%
TTCTGCCTCTTGTGGATAAG+GGG - chr8_1:71523176-71523195 Msa1211800:intron 45.0%
GATCAAAAGATGGGTGGATC+TGG - chr8_1:71521679-71521698 Msa1211800:three_prime_UTR 45.0%
ACTCCAGATATGCTTCGGTT+AGG - chr8_1:71522024-71522043 Msa1211800:CDS 45.0%
CTCCAGATATGCTTCGGTTA+GGG - chr8_1:71522025-71522044 Msa1211800:CDS 45.0%
TAGCCACTGTTGATCATCCA+CGG + chr8_1:71522069-71522088 None:intergenic 45.0%
TATCATCTGCGTGTGTAAGC+TGG - chr8_1:71522339-71522358 Msa1211800:intron 45.0%
! GTATATCTGCATTGGCCCTT+TGG - chr8_1:71522463-71522482 Msa1211800:intron 45.0%
GTCACTACAAGCACATCCAA+AGG + chr8_1:71522482-71522501 None:intergenic 45.0%
! GTGAAGACGTCCTTTTTCCT+TGG + chr8_1:71522921-71522940 None:intergenic 45.0%
TTTATCAGCCTGCAGAGTGA+TGG + chr8_1:71523093-71523112 None:intergenic 45.0%
CTTCTGCCTCTTGTGGATAA+GGG - chr8_1:71523175-71523194 Msa1211800:intron 45.0%
TTCTGCCTCTTGTGGATAAG+GGG - chr8_1:71523176-71523195 Msa1211800:intron 45.0%
GGGCTTGACTATCGAGTGAA+AGG + chr8_1:71521540-71521559 None:intergenic 50.0%
AAGATGGGTGGATCTGGATC+TGG - chr8_1:71521685-71521704 Msa1211800:three_prime_UTR 50.0%
!!! TGGTGCCGGTAGTTTTCTCA+TGG - chr8_1:71521711-71521730 Msa1211800:three_prime_UTR 50.0%
TGCCCTAACCGAAGCATATC+TGG + chr8_1:71522030-71522049 None:intergenic 50.0%
AATTGAGAGCAAGTCTCCCG+TGG - chr8_1:71522049-71522068 Msa1211800:CDS 50.0%
AGCCACTGTTGATCATCCAC+GGG + chr8_1:71522068-71522087 None:intergenic 50.0%
TTGGTTACCTGCCAATCTGC+AGG - chr8_1:71522364-71522383 Msa1211800:intron 50.0%
TGGTTACCTGCCAATCTGCA+GGG - chr8_1:71522365-71522384 Msa1211800:intron 50.0%
GAATAACCCTGCAGATTGGC+AGG + chr8_1:71522374-71522393 None:intergenic 50.0%
ATAAGGAAGGACCAACCAGC+AGG + chr8_1:71522817-71522836 None:intergenic 50.0%
!! GTCTGAGGATTGGCCAAGAA+GGG + chr8_1:71522879-71522898 None:intergenic 50.0%
TAACTGTGCCATCACTCTGC+AGG - chr8_1:71523082-71523101 Msa1211800:intron 50.0%
CTTGTAGCTTCTGCCTCTTG+TGG - chr8_1:71523168-71523187 Msa1211800:intron 50.0%
GCTTCTGCCTCTTGTGGATA+AGG - chr8_1:71523174-71523193 Msa1211800:intron 50.0%
TCTGCCTCTTGTGGATAAGG+GGG - chr8_1:71523177-71523196 Msa1211800:intron 50.0%
GGGCTTGACTATCGAGTGAA+AGG + chr8_1:71521540-71521559 None:intergenic 50.0%
AAGATGGGTGGATCTGGATC+TGG - chr8_1:71521685-71521704 Msa1211800:three_prime_UTR 50.0%
!!! TGGTGCCGGTAGTTTTCTCA+TGG - chr8_1:71521711-71521730 Msa1211800:three_prime_UTR 50.0%
TGCCCTAACCGAAGCATATC+TGG + chr8_1:71522030-71522049 None:intergenic 50.0%
AATTGAGAGCAAGTCTCCCG+TGG - chr8_1:71522049-71522068 Msa1211800:CDS 50.0%
AGCCACTGTTGATCATCCAC+GGG + chr8_1:71522068-71522087 None:intergenic 50.0%
TTGGTTACCTGCCAATCTGC+AGG - chr8_1:71522364-71522383 Msa1211800:intron 50.0%
TGGTTACCTGCCAATCTGCA+GGG - chr8_1:71522365-71522384 Msa1211800:intron 50.0%
GAATAACCCTGCAGATTGGC+AGG + chr8_1:71522374-71522393 None:intergenic 50.0%
ATAAGGAAGGACCAACCAGC+AGG + chr8_1:71522817-71522836 None:intergenic 50.0%
!! GTCTGAGGATTGGCCAAGAA+GGG + chr8_1:71522879-71522898 None:intergenic 50.0%
TAACTGTGCCATCACTCTGC+AGG - chr8_1:71523082-71523101 Msa1211800:intron 50.0%
CTTGTAGCTTCTGCCTCTTG+TGG - chr8_1:71523168-71523187 Msa1211800:intron 50.0%
GCTTCTGCCTCTTGTGGATA+AGG - chr8_1:71523174-71523193 Msa1211800:intron 50.0%
TCTGCCTCTTGTGGATAAGG+GGG - chr8_1:71523177-71523196 Msa1211800:intron 50.0%
GGTGGATCTGGATCTGGATC+TGG - chr8_1:71521691-71521710 Msa1211800:three_prime_UTR 55.0%
TCTGGATCTGGATCTGGTGC+CGG - chr8_1:71521697-71521716 Msa1211800:three_prime_UTR 55.0%
CTCCCGTGGATGATCAACAG+TGG - chr8_1:71522063-71522082 Msa1211800:CDS 55.0%
! CTGCGTGTGTAAGCTGGCTT+TGG - chr8_1:71522345-71522364 Msa1211800:intron 55.0%
!! GGTCTGAGGATTGGCCAAGA+AGG + chr8_1:71522880-71522899 None:intergenic 55.0%
CGAACCCCCTTATCCACAAG+AGG + chr8_1:71523184-71523203 None:intergenic 55.0%
GGTGGATCTGGATCTGGATC+TGG - chr8_1:71521691-71521710 Msa1211800:three_prime_UTR 55.0%
TCTGGATCTGGATCTGGTGC+CGG - chr8_1:71521697-71521716 Msa1211800:three_prime_UTR 55.0%
CTCCCGTGGATGATCAACAG+TGG - chr8_1:71522063-71522082 Msa1211800:CDS 55.0%
! CTGCGTGTGTAAGCTGGCTT+TGG - chr8_1:71522345-71522364 Msa1211800:intron 55.0%
!! GGTCTGAGGATTGGCCAAGA+AGG + chr8_1:71522880-71522899 None:intergenic 55.0%
CGAACCCCCTTATCCACAAG+AGG + chr8_1:71523184-71523203 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 71521470 71523559 71521470 ID=Msa1211800;Name=Msa1211800
chr8_1 mRNA 71521470 71523559 71521470 ID=Msa1211800-mRNA-1;Parent=Msa1211800;Name=Msa1211800-mRNA-1;_AED=0.20;_eAED=0.20;_QI=218|1|1|1|1|1|5|399|175
chr8_1 exon 71521470 71521925 71521470 ID=Msa1211800-mRNA-1:exon:24119;Parent=Msa1211800-mRNA-1
chr8_1 exon 71522007 71522250 71522007 ID=Msa1211800-mRNA-1:exon:24118;Parent=Msa1211800-mRNA-1
chr8_1 exon 71522876 71522998 71522876 ID=Msa1211800-mRNA-1:exon:24117;Parent=Msa1211800-mRNA-1
chr8_1 exon 71523112 71523138 71523112 ID=Msa1211800-mRNA-1:exon:24116;Parent=Msa1211800-mRNA-1
chr8_1 exon 71523265 71523559 71523265 ID=Msa1211800-mRNA-1:exon:24115;Parent=Msa1211800-mRNA-1
chr8_1 five_prime_UTR 71523342 71523559 71523342 ID=Msa1211800-mRNA-1:five_prime_utr;Parent=Msa1211800-mRNA-1
chr8_1 CDS 71523265 71523341 71523265 ID=Msa1211800-mRNA-1:cds;Parent=Msa1211800-mRNA-1
chr8_1 CDS 71523112 71523138 71523112 ID=Msa1211800-mRNA-1:cds;Parent=Msa1211800-mRNA-1
chr8_1 CDS 71522876 71522998 71522876 ID=Msa1211800-mRNA-1:cds;Parent=Msa1211800-mRNA-1
chr8_1 CDS 71522007 71522250 71522007 ID=Msa1211800-mRNA-1:cds;Parent=Msa1211800-mRNA-1
chr8_1 CDS 71521869 71521925 71521869 ID=Msa1211800-mRNA-1:cds;Parent=Msa1211800-mRNA-1
chr8_1 three_prime_UTR 71521470 71521868 71521470 ID=Msa1211800-mRNA-1:three_prime_utr;Parent=Msa1211800-mRNA-1
Gene Sequence

>Msa1211800

ATGGGTGGATCTGGATCTGGATCTGGTGCCGGTAGTTTTCTCATGGTTCTTCTCAGAAATTTTGATGTACTTGCTGGGCCTGTGATTAGTCTTGTTTATCCTTTATATGCTTCGGTTAGGGCAATTGAGAGCAAGTCTCCCGTGGATGATCAACAGTGGCTAACCTATTGGGTTCTGTATTCATTGATCACTCTTTTTGAACTTACTTTTGCCAAAATTCTTGAATGGATCCCTATATGGCCATATGCAAAGTTTATTCTAACCTGCTGGTTGGTCCTTCCTTATTTTACTGGTGCTGCTTACGTTTATGAGCATTATGTAAGACCCTTCTTGGCCAATCCTCAGACCATTAACATCTGGTATGTTCCAAGGAAAAAGGACGTCTTCACTAAACCAGATGACATTATAACTGCTGCAGAGAAGTACATCCAAGAGAATGGAACAGAAGCATTTGAGAATCTGATCCATAGGGCTGATAAATCTAAATGGGGCGGTAGTCACCATACAATGTATGATGAGACCTATTGA
Protein sequence

>Msa1211800

MGGSGSGSGAGSFLMVLLRNFDVLAGPVISLVYPLYASVRAIESKSPVDDQQWLTYWVLYSLITLFELTFAKILEWIPIWPYAKFILTCWLVLPYFTGAAYVYEHYVRPFLANPQTINIWYVPRKKDVFTKPDDIITAAEKYIQENGTEAFENLIHRADKSKWGGSHHTMYDETY*