AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1220730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220730 MtrunA17_Chr4g0072101 96.855 159 5 0 55 213 5 163 6.79e-114 322
Msa1220730 MtrunA17_Chr2g0298071 41.860 258 100 9 1 213 1 253 2.26e-46 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1220730 AT5G57340.1 40.164 122 48 5 55 176 57 153 1.94e-14 70.1
Msa1220730 AT5G57340.2 40.164 122 48 5 55 176 75 171 2.31e-14 70.1

Find 57 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGTTGCTACAAGATTTA+TGG 0.154976 8_1:+84013242 None:intergenic
ATTTATGGAACATTCAATTT+TGG 0.200899 8_1:+84013257 None:intergenic
TATAGTCCTGTGATGGTTAT+TGG 0.284417 8_1:+84013146 None:intergenic
TCTAATATGAAACTTCAATT+AGG 0.300464 8_1:-84012307 Msa1220730:CDS
ATTCTGCTACTTCTTATTAC+AGG 0.304234 8_1:-84014737 Msa1220730:intron
TGTCACACTGTTGCTCCTAA+TGG 0.328969 8_1:+84012261 None:intergenic
GTCACACTGTTGCTCCTAAT+GGG 0.332519 8_1:+84012262 None:intergenic
ATTCTCCTTGAGAGACAAAA+AGG 0.342116 8_1:+84014869 None:intergenic
ACTGTGTAAATTAATAATAT+TGG 0.379818 8_1:-84014829 Msa1220730:five_prime_UTR
TTCTATGATATATCTTTCTC+AGG 0.403651 8_1:-84014919 Msa1220730:five_prime_UTR
TAACTTATTGCTCTTTACTA+AGG 0.413153 8_1:+84013224 None:intergenic
AGCAGAATTGCAGCTTTCTC+TGG 0.413595 8_1:+84014752 None:intergenic
TCTTTGTTGTTTCTTAGTGA+AGG 0.423743 8_1:-84014966 Msa1220730:five_prime_UTR
CAAATTTAAAACTATAATCA+TGG 0.423848 8_1:+84014997 None:intergenic
ATTAATAATTCATGCTTGAC+AGG 0.425827 8_1:+84015023 None:intergenic
ATATTCATACTGTAATATAT+AGG 0.433605 8_1:-84015143 Msa1220730:five_prime_UTR
CTTTGTTGTTTCTTAGTGAA+GGG 0.433661 8_1:-84014965 Msa1220730:five_prime_UTR
TATGTTGTTTCTATCCATGA+TGG 0.434780 8_1:+84013104 None:intergenic
GTGACACAACCAACCCCATT+AGG 0.451171 8_1:-84012276 Msa1220730:CDS
ATATTATTAATTTACACAGT+TGG 0.463006 8_1:+84014832 None:intergenic
AATAAAGTAGAAACTAATCT+AGG 0.470222 8_1:+84012123 None:intergenic
ATGTTGTTTCTATCCATGAT+GGG 0.484028 8_1:+84013105 None:intergenic
TCATATTAGAATTCTTAAGT+TGG 0.490514 8_1:+84012320 None:intergenic
AGTATTATTATCAGAACAAA+GGG 0.491849 8_1:-84013176 Msa1220730:CDS
TGTAACCTTTCAACTCTACA+TGG 0.503587 8_1:-84012180 Msa1220730:three_prime_UTR
GCTCTGAAATGCAGCAGTGT+TGG 0.531063 8_1:+84012414 None:intergenic
CTGTGGGTGGAGTGAATGTG+TGG 0.533528 8_1:-84013200 Msa1220730:CDS
AAATGCAGCAGTGTTGGAAC+TGG 0.538023 8_1:+84012420 None:intergenic
ACTGTTGCTCCTAATGGGGT+TGG 0.541229 8_1:+84012267 None:intergenic
TATTGATTATAGTCCTGTGA+TGG 0.542206 8_1:+84013139 None:intergenic
TAAATTGCAAGGTCCCATCA+TGG 0.552827 8_1:-84013118 Msa1220730:CDS
AAATTTAAAACTATAATCAT+GGG 0.553892 8_1:+84014998 None:intergenic
GGCTGACCAATAACCATCAC+AGG 0.553998 8_1:-84013152 Msa1220730:CDS
GTGGTCCATGTAGAGTTGAA+AGG 0.563193 8_1:+84012175 None:intergenic
GGCATTGATGAAGGTGAGGT+AGG 0.564515 8_1:-84012445 Msa1220730:CDS
TCATCACTTGGCATTGATGA+AGG 0.574340 8_1:-84012454 Msa1220730:CDS
ACTATAATCAATAAATTGCA+AGG 0.575944 8_1:-84013129 Msa1220730:CDS
GATTTGACACTAAGCATAGG+AGG 0.578450 8_1:-84012346 Msa1220730:CDS
AAAGAGCAATAAGTTAGCTG+TGG 0.583848 8_1:-84013217 Msa1220730:CDS
GCATTGATGAAGGTGAGGTA+GGG 0.591301 8_1:-84012444 Msa1220730:CDS
TGATCTTGAAAAGCCAGCTG+TGG 0.591394 8_1:-84012494 Msa1220730:CDS
AGCAATAAGTTAGCTGTGGG+TGG 0.591461 8_1:-84013213 Msa1220730:CDS
AACAGTGTGACATTTACTCA+AGG 0.600555 8_1:-84012250 Msa1220730:CDS
AAAGATCAATCCAACAACAT+GGG 0.607629 8_1:+84014892 None:intergenic
AAGATCAATCCAACAACATG+GGG 0.615061 8_1:+84014893 None:intergenic
GAGTATTATTATCAGAACAA+AGG 0.615462 8_1:-84013177 Msa1220730:CDS
GAAAGATCAATCCAACAACA+TGG 0.619463 8_1:+84014891 None:intergenic
AAGAGCAATAAGTTAGCTGT+GGG 0.620145 8_1:-84013216 Msa1220730:CDS
CATGAACCATCAAAATTCAG+TGG 0.623614 8_1:+84012156 None:intergenic
TGTGGGTGGAGTGAATGTGT+GGG 0.624316 8_1:-84013199 Msa1220730:CDS
GTAGATTTGACACTAAGCAT+AGG 0.634592 8_1:-84012349 Msa1220730:CDS
TCACACTGTTGCTCCTAATG+GGG 0.647566 8_1:+84012263 None:intergenic
ATTATTATCAGAACAAAGGG+AGG 0.652018 8_1:-84013173 Msa1220730:CDS
ACTTGGCATTGATGAAGGTG+AGG 0.653122 8_1:-84012449 Msa1220730:CDS
AAAGACAATGCAAATGCATG+AGG 0.654368 8_1:-84013070 Msa1220730:CDS
TGAGGACATCTTCAAACACC+AGG 0.656291 8_1:-84013052 Msa1220730:intron
AGATCAATCCAACAACATGG+GGG 0.746907 8_1:+84014894 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAAAACTATAATCAT+GGG + chr8_1:84012244-84012263 None:intergenic 10.0%
!!! TAATGGATATAATTATTTTT+TGG - chr8_1:84012604-84012623 Msa1220730:CDS 10.0%
!! AAAAAAATAGAAAAAAAAAC+TGG + chr8_1:84013260-84013279 None:intergenic 10.0%
!! ATATTCATACTGTAATATAT+AGG - chr8_1:84012096-84012115 Msa1220730:three_prime_UTR 15.0%
!!! CAAATTTAAAACTATAATCA+TGG + chr8_1:84012245-84012264 None:intergenic 15.0%
!! ATATTATTAATTTACACAGT+TGG + chr8_1:84012410-84012429 None:intergenic 15.0%
!! ACTGTGTAAATTAATAATAT+TGG - chr8_1:84012410-84012429 Msa1220730:CDS 15.0%
!! ATAATTATATCCATTACATT+AGG + chr8_1:84012600-84012619 None:intergenic 15.0%
!!! TGAACAAATGTTTTAAAAAT+CGG + chr8_1:84013386-84013405 None:intergenic 15.0%
!!! TGGTTTTAATTTTTGTATTT+AGG - chr8_1:84013951-84013970 Msa1220730:intron 15.0%
!!! TTAATTTTTGTATTTAGGTA+TGG - chr8_1:84013956-84013975 Msa1220730:intron 15.0%
!!! TAATTTTTGTATTTAGGTAT+GGG - chr8_1:84013957-84013976 Msa1220730:intron 15.0%
!!! TTAACTTGAATTATGTATTT+GGG + chr8_1:84014250-84014269 None:intergenic 15.0%
!!! ATTAACTTGAATTATGTATT+TGG + chr8_1:84014251-84014270 None:intergenic 15.0%
!!! ATTTTCAAGTTTACTAAAAT+TGG - chr8_1:84014371-84014390 Msa1220730:intron 15.0%
!!! AAATTAGACAACAAAATTTT+TGG - chr8_1:84014681-84014700 Msa1220730:intron 15.0%
!!! GTATAACTAATTCAAGTTAA+TGG + chr8_1:84012574-84012593 None:intergenic 20.0%
!!! ATTATTTTTTGGTGTTTATG+TGG - chr8_1:84012615-84012634 Msa1220730:CDS 20.0%
!!! TTATTTTTTATGTATCACCA+TGG + chr8_1:84012707-84012726 None:intergenic 20.0%
!! AGTGAAAAAATTTATGAGAT+GGG + chr8_1:84012889-84012908 None:intergenic 20.0%
!! TAGTGAAAAAATTTATGAGA+TGG + chr8_1:84012890-84012909 None:intergenic 20.0%
!! GTATTTATTTATTTGTCATC+CGG - chr8_1:84013292-84013311 Msa1220730:intron 20.0%
!!! CTTATTTTCATAAGTTTTTC+AGG - chr8_1:84013820-84013839 Msa1220730:intron 20.0%
!!! TTTATTTTCATGCTGTTTAT+AGG + chr8_1:84013850-84013869 None:intergenic 20.0%
!!! ATTTATGGAACATTCAATTT+TGG + chr8_1:84013985-84014004 None:intergenic 20.0%
!! AGTATTATTATCAGAACAAA+GGG - chr8_1:84014063-84014082 Msa1220730:intron 20.0%
!! ACTATAATCAATAAATTGCA+AGG - chr8_1:84014110-84014129 Msa1220730:intron 20.0%
!! AAAAGTAAAACCATAGTAAT+TGG - chr8_1:84014309-84014328 Msa1220730:intron 20.0%
!!! AATTTTAATCTCTACTAGTT+AGG - chr8_1:84014572-84014591 Msa1220730:intron 20.0%
!!! ATAGAAAAACAATTTTCAAG+AGG - chr8_1:84014719-84014738 Msa1220730:intron 20.0%
!!! TGATGATTTTAATGAAGAAT+TGG - chr8_1:84014865-84014884 Msa1220730:five_prime_UTR 20.0%
!! TCATATTAGAATTCTTAAGT+TGG + chr8_1:84014922-84014941 None:intergenic 20.0%
!! TCTAATATGAAACTTCAATT+AGG - chr8_1:84014932-84014951 Msa1220730:five_prime_UTR 20.0%
!! AATAAAGTAGAAACTAATCT+AGG + chr8_1:84015119-84015138 None:intergenic 20.0%
! ATTAATAATTCATGCTTGAC+AGG + chr8_1:84012219-84012238 None:intergenic 25.0%
! TTCTATGATATATCTTTCTC+AGG - chr8_1:84012320-84012339 Msa1220730:CDS 25.0%
! GTTATACAATCCTAATGTAA+TGG - chr8_1:84012587-84012606 Msa1220730:CDS 25.0%
! GAGTTTACAATTAAAAGTCA+AGG + chr8_1:84012770-84012789 None:intergenic 25.0%
! AAATTATTGAGCTATGTAGA+AGG + chr8_1:84012819-84012838 None:intergenic 25.0%
! TGAATTAAGTACTACTGTTA+AGG - chr8_1:84012973-84012992 Msa1220730:intron 25.0%
! GAATTAAGTACTACTGTTAA+GGG - chr8_1:84012974-84012993 Msa1220730:intron 25.0%
! GTAACATGAACAATAGATTT+AGG + chr8_1:84013074-84013093 None:intergenic 25.0%
! ATTGACATGAAAATTGAAGT+AGG + chr8_1:84013099-84013118 None:intergenic 25.0%
! CAATTCAATACAAATTCTCT+TGG - chr8_1:84013425-84013444 Msa1220730:intron 25.0%
! AACTGAAACAAAAATCCAAA+TGG + chr8_1:84013627-84013646 None:intergenic 25.0%
! GAAACTAGAAACCAAAATTT+AGG + chr8_1:84013713-84013732 None:intergenic 25.0%
! TAACTTATTGCTCTTTACTA+AGG + chr8_1:84014018-84014037 None:intergenic 25.0%
! GAGTATTATTATCAGAACAA+AGG - chr8_1:84014062-84014081 Msa1220730:intron 25.0%
! TTCATGTAATGTAACATTTG+TGG + chr8_1:84014291-84014310 None:intergenic 25.0%
!! ACAAATTTTCGTTTACTACA+AGG - chr8_1:84014405-84014424 Msa1220730:intron 25.0%
!!! TAACCCTTATTGATTTTGAA+AGG - chr8_1:84014532-84014551 Msa1220730:intron 25.0%
! TGTCCTTTCAAAATCAATAA+GGG + chr8_1:84014538-84014557 None:intergenic 25.0%
! ATGTCCTTTCAAAATCAATA+AGG + chr8_1:84014539-84014558 None:intergenic 25.0%
!! AGTTAGGAATCATATGTTTT+TGG - chr8_1:84014588-84014607 Msa1220730:intron 25.0%
!! TGTTTTTCTATGTCATTCAT+TGG + chr8_1:84014710-84014729 None:intergenic 25.0%
!! TCTTTGTTGTTTCTTAGTGA+AGG - chr8_1:84012273-84012292 Msa1220730:CDS 30.0%
!! CTTTGTTGTTTCTTAGTGAA+GGG - chr8_1:84012274-84012293 Msa1220730:CDS 30.0%
AAAGATCAATCCAACAACAT+GGG + chr8_1:84012350-84012369 None:intergenic 30.0%
ATTGGAGTTGAGTGTAAAAA+TGG - chr8_1:84012428-84012447 Msa1220730:CDS 30.0%
! ATTCTGCTACTTCTTATTAC+AGG - chr8_1:84012502-84012521 Msa1220730:CDS 30.0%
AATATGTATCTGTGAAGTGT+AGG + chr8_1:84012546-84012565 None:intergenic 30.0%
TTTAATGCTTCTTTAGTCCA+TGG - chr8_1:84012687-84012706 Msa1220730:intron 30.0%
ACATAAACTTAGACACTATG+AGG + chr8_1:84012792-84012811 None:intergenic 30.0%
TACTACTGTTAAGGGATTTA+TGG - chr8_1:84012982-84013001 Msa1220730:intron 30.0%
TAACAATGAAACCAACATGT+TGG + chr8_1:84013051-84013070 None:intergenic 30.0%
!!! TGTTTTAATTGGTTCAACCA+TGG + chr8_1:84013323-84013342 None:intergenic 30.0%
GAACCAATTAAAACATGACT+TGG - chr8_1:84013328-84013347 Msa1220730:intron 30.0%
!!! TCTCCAAGTCATGTTTTAAT+TGG + chr8_1:84013334-84013353 None:intergenic 30.0%
!!! AATGTTTTAAAAATCGGTCC+AGG + chr8_1:84013380-84013399 None:intergenic 30.0%
!! TCCACTTTTGGTTATTAAAG+TGG - chr8_1:84013467-84013486 Msa1220730:intron 30.0%
TCCACTTTAATAACCAAAAG+TGG + chr8_1:84013471-84013490 None:intergenic 30.0%
! AATTGAAACAGGCAACTTTT+AGG + chr8_1:84013547-84013566 None:intergenic 30.0%
CTGTTTCAATTCACCAAAAA+GGG - chr8_1:84013556-84013575 Msa1220730:intron 30.0%
TTTGTTTCAGTTTCTTGCTA+TGG - chr8_1:84013635-84013654 Msa1220730:intron 30.0%
TTTACCTAGTTTGATCTCAA+CGG - chr8_1:84013685-84013704 Msa1220730:intron 30.0%
GTTGAAAACAGTCTATAGAT+AGG + chr8_1:84013801-84013820 None:intergenic 30.0%
AACCATTGCTAATCATGTAA+GGG + chr8_1:84013936-84013955 None:intergenic 30.0%
AAACCATTGCTAATCATGTA+AGG + chr8_1:84013937-84013956 None:intergenic 30.0%
TAAGGTTGCTACAAGATTTA+TGG + chr8_1:84014000-84014019 None:intergenic 30.0%
ATTATTATCAGAACAAAGGG+AGG - chr8_1:84014066-84014085 Msa1220730:intron 30.0%
! TATTGATTATAGTCCTGTGA+TGG + chr8_1:84014103-84014122 None:intergenic 30.0%
!! ATGTTGTTTCTATCCATGAT+GGG + chr8_1:84014137-84014156 None:intergenic 30.0%
!! TATGTTGTTTCTATCCATGA+TGG + chr8_1:84014138-84014157 None:intergenic 30.0%
! CATTTGCATTGTCTTTTTGA+TGG + chr8_1:84014165-84014184 None:intergenic 30.0%
TTCAGTGATATGAAATCTTC+AGG + chr8_1:84014216-84014235 None:intergenic 30.0%
GTAAAACCATAGTAATTGGT+AGG - chr8_1:84014313-84014332 Msa1220730:intron 30.0%
TATAAGCCTACCAATTACTA+TGG + chr8_1:84014322-84014341 None:intergenic 30.0%
!!! CATATGTTTTTGGAATTTGC+AGG - chr8_1:84014598-84014617 Msa1220730:intron 30.0%
! ACTTTTATTGGATCATCACT+TGG - chr8_1:84014773-84014792 Msa1220730:five_prime_UTR 30.0%
AAGATCAATCCAACAACATG+GGG + chr8_1:84012349-84012368 None:intergenic 35.0%
GAAAGATCAATCCAACAACA+TGG + chr8_1:84012351-84012370 None:intergenic 35.0%
! TCTTTCCTTTTTGTCTCTCA+AGG - chr8_1:84012365-84012384 Msa1220730:CDS 35.0%
ATTCTCCTTGAGAGACAAAA+AGG + chr8_1:84012373-84012392 None:intergenic 35.0%
GTGGAGTTTCCAAACATATA+TGG - chr8_1:84012634-84012653 Msa1220730:intron 35.0%
ACACATGCACCATATATGTT+TGG + chr8_1:84012646-84012665 None:intergenic 35.0%
TGATTCCCCCACATAAATTT+AGG - chr8_1:84012910-84012929 Msa1220730:intron 35.0%
ACTTAATTCATCTGCAACTC+TGG + chr8_1:84012963-84012982 None:intergenic 35.0%
ATAATCACAGACCAACATGT+TGG - chr8_1:84013037-84013056 Msa1220730:intron 35.0%
AGAACTCTCGATTGAACTAT+AGG - chr8_1:84013201-84013220 Msa1220730:CDS 35.0%
!! GAACTATAGGTTGATTGAAC+CGG - chr8_1:84013214-84013233 Msa1220730:CDS 35.0%
!! CCTTTTTGGTGAATTGAAAC+AGG + chr8_1:84013558-84013577 None:intergenic 35.0%
CCTGTTTCAATTCACCAAAA+AGG - chr8_1:84013555-84013574 Msa1220730:intron 35.0%
TAATGTCAGCTTATCATGAC+TGG - chr8_1:84013600-84013619 Msa1220730:intron 35.0%
! TCTCAACGGTTCCTAAATTT+TGG - chr8_1:84013699-84013718 Msa1220730:intron 35.0%
TCAAGCTACAATGTGAAGAT+GGG + chr8_1:84013768-84013787 None:intergenic 35.0%
TACCCTTACATGATTAGCAA+TGG - chr8_1:84013931-84013950 Msa1220730:intron 35.0%
AAAGAGCAATAAGTTAGCTG+TGG - chr8_1:84014022-84014041 Msa1220730:intron 35.0%
AAGAGCAATAAGTTAGCTGT+GGG - chr8_1:84014023-84014042 Msa1220730:intron 35.0%
TATAGTCCTGTGATGGTTAT+TGG + chr8_1:84014096-84014115 None:intergenic 35.0%
AAAGACAATGCAAATGCATG+AGG - chr8_1:84014169-84014188 Msa1220730:intron 35.0%
TATGAAATCTTCAGGATACC+TGG + chr8_1:84014208-84014227 None:intergenic 35.0%
CATTTGTGGCACAGATTAAA+TGG + chr8_1:84014277-84014296 None:intergenic 35.0%
!!! ATAAAAGTGTTTTCCACAGC+TGG + chr8_1:84014761-84014780 None:intergenic 35.0%
! GCTGTGGAAAACACTTTTAT+TGG - chr8_1:84014761-84014780 Msa1220730:five_prime_UTR 35.0%
GTAGATTTGACACTAAGCAT+AGG - chr8_1:84014890-84014909 Msa1220730:five_prime_UTR 35.0%
AACAGTGTGACATTTACTCA+AGG - chr8_1:84014989-84015008 Msa1220730:five_prime_UTR 35.0%
TGTAACCTTTCAACTCTACA+TGG - chr8_1:84015059-84015078 Msa1220730:five_prime_UTR 35.0%
CATGAACCATCAAAATTCAG+TGG + chr8_1:84015086-84015105 None:intergenic 35.0%
AGGCTGTTGCAAGAAAAAGA+TGG + chr8_1:84012199-84012218 None:intergenic 40.0%
AGATCAATCCAACAACATGG+GGG + chr8_1:84012348-84012367 None:intergenic 40.0%
GGAGTTGAGTGTAAAAATGG+TGG - chr8_1:84012431-84012450 Msa1220730:CDS 40.0%
AGTGTAAAAATGGTGGCATG+AGG - chr8_1:84012438-84012457 Msa1220730:CDS 40.0%
GTGTAAAAATGGTGGCATGA+GGG - chr8_1:84012439-84012458 Msa1220730:CDS 40.0%
TGAGGCCTAAATTTATGTGG+GGG + chr8_1:84012918-84012937 None:intergenic 40.0%
GTGAGGCCTAAATTTATGTG+GGG + chr8_1:84012919-84012938 None:intergenic 40.0%
GGTGAGGCCTAAATTTATGT+GGG + chr8_1:84012920-84012939 None:intergenic 40.0%
AGGTGAGGCCTAAATTTATG+TGG + chr8_1:84012921-84012940 None:intergenic 40.0%
AGAAAATCCAACACAAGGTG+AGG + chr8_1:84012936-84012955 None:intergenic 40.0%
! ACCTTGTGTTGGATTTTCTG+AGG - chr8_1:84012937-84012956 Msa1220730:intron 40.0%
TTATATGATGACAGCAGCAG+TGG + chr8_1:84013021-84013040 None:intergenic 40.0%
! TCTCTATCTCTCTCCACTTT+TGG - chr8_1:84013455-84013474 Msa1220730:intron 40.0%
!! GCAACTTTTAGGAAGGTTTC+TGG + chr8_1:84013536-84013555 None:intergenic 40.0%
!! GAAACAGGCAACTTTTAGGA+AGG + chr8_1:84013543-84013562 None:intergenic 40.0%
! GATACTGTAACTGCCCTTTT+TGG + chr8_1:84013572-84013591 None:intergenic 40.0%
! CTTATCATGACTGGTCCATT+TGG - chr8_1:84013609-84013628 Msa1220730:intron 40.0%
!! TCTTGCTATGGTACTGATTG+TGG - chr8_1:84013647-84013666 Msa1220730:intron 40.0%
CAAGCTACAATGTGAAGATG+GGG + chr8_1:84013767-84013786 None:intergenic 40.0%
CTCAAGCTACAATGTGAAGA+TGG + chr8_1:84013769-84013788 None:intergenic 40.0%
TCTTCACATTGTAGCTTGAG+AGG - chr8_1:84013769-84013788 Msa1220730:intron 40.0%
TAAATTGCAAGGTCCCATCA+TGG - chr8_1:84014121-84014140 Msa1220730:intron 40.0%
!! TCATCACTTGGCATTGATGA+AGG - chr8_1:84014785-84014804 Msa1220730:five_prime_UTR 40.0%
GATTTGACACTAAGCATAGG+AGG - chr8_1:84014893-84014912 Msa1220730:five_prime_UTR 40.0%
! CATGGACCACTGAATTTTGA+TGG - chr8_1:84015077-84015096 Msa1220730:five_prime_UTR 40.0%
TAAAAATGGTGGCATGAGGG+AGG - chr8_1:84012442-84012461 Msa1220730:CDS 45.0%
AGCAGAATTGCAGCTTTCTC+TGG + chr8_1:84012490-84012509 None:intergenic 45.0%
ATTTAGGCCTCACCTTGTGT+TGG - chr8_1:84012926-84012945 Msa1220730:intron 45.0%
GCCTCAGAAAATCCAACACA+AGG + chr8_1:84012941-84012960 None:intergenic 45.0%
ATTGGTCGAACTGGCTGATT+CGG + chr8_1:84013135-84013154 None:intergenic 45.0%
TTCGGTTCAATTGGTCGAAC+TGG + chr8_1:84013144-84013163 None:intergenic 45.0%
!!! TGGCTGGTTTTCGGTTCAAT+TGG + chr8_1:84013153-84013172 None:intergenic 45.0%
TGAACCATCTGCTGTTGAAC+CGG + chr8_1:84013236-84013255 None:intergenic 45.0%
TTTGTCATCCGGTTCAACCA+TGG - chr8_1:84013303-84013322 Msa1220730:intron 45.0%
TGGTTCAACCATGGTTGAAC+CGG + chr8_1:84013314-84013333 None:intergenic 45.0%
AATCGGTCCAGGAATCGAAT+CGG + chr8_1:84013369-84013388 None:intergenic 45.0%
AGTGGAGAGAGATAGAGAGA+GGG + chr8_1:84013453-84013472 None:intergenic 45.0%
AAGTGGAGAGAGATAGAGAG+AGG + chr8_1:84013454-84013473 None:intergenic 45.0%
!! ATGGTACTGATTGTGGTCTC+AGG - chr8_1:84013654-84013673 Msa1220730:intron 45.0%
GGAACCGTTGAGATCAAACT+AGG + chr8_1:84013692-84013711 None:intergenic 45.0%
AGCAATAAGTTAGCTGTGGG+TGG - chr8_1:84014026-84014045 Msa1220730:intron 45.0%
TGAGGACATCTTCAAACACC+AGG - chr8_1:84014187-84014206 Msa1220730:intron 45.0%
TGATCTTGAAAAGCCAGCTG+TGG - chr8_1:84014745-84014764 Msa1220730:five_prime_UTR 45.0%
!! ACTTGGCATTGATGAAGGTG+AGG - chr8_1:84014790-84014809 Msa1220730:five_prime_UTR 45.0%
! GCATTGATGAAGGTGAGGTA+GGG - chr8_1:84014795-84014814 Msa1220730:five_prime_UTR 45.0%
! AAATGCAGCAGTGTTGGAAC+TGG + chr8_1:84014822-84014841 None:intergenic 45.0%
TCACACTGTTGCTCCTAATG+GGG + chr8_1:84014979-84014998 None:intergenic 45.0%
GTCACACTGTTGCTCCTAAT+GGG + chr8_1:84014980-84014999 None:intergenic 45.0%
TGTCACACTGTTGCTCCTAA+TGG + chr8_1:84014981-84015000 None:intergenic 45.0%
GTGGTCCATGTAGAGTTGAA+AGG + chr8_1:84015067-84015086 None:intergenic 45.0%
!! ATAAAAAAAGTAAAAAAATT+AGG - chr8_1:84013865-84013884 Msa1220730:intron 5.0%
!!! CCAGCGAATTGGCTGGTTTT+CGG + chr8_1:84013162-84013181 None:intergenic 50.0%
AAACCAGCCAATTCGCTGGT+TGG - chr8_1:84013163-84013182 Msa1220730:CDS 50.0%
CCAACAACCAACCAGCGAAT+TGG + chr8_1:84013173-84013192 None:intergenic 50.0%
! CCAATTCGCTGGTTGGTTGT+TGG - chr8_1:84013170-84013189 Msa1220730:CDS 50.0%
! TGAACCGGTTCAACAGCAGA+TGG - chr8_1:84013229-84013248 Msa1220730:CDS 50.0%
TGGAGAGAGATAGAGAGAGG+GGG + chr8_1:84013451-84013470 None:intergenic 50.0%
GTGGAGAGAGATAGAGAGAG+GGG + chr8_1:84013452-84013471 None:intergenic 50.0%
TGTGGGTGGAGTGAATGTGT+GGG - chr8_1:84014040-84014059 Msa1220730:intron 50.0%
GGCTGACCAATAACCATCAC+AGG - chr8_1:84014087-84014106 Msa1220730:intron 50.0%
!! GGCATTGATGAAGGTGAGGT+AGG - chr8_1:84014794-84014813 Msa1220730:five_prime_UTR 50.0%
!! GCTCTGAAATGCAGCAGTGT+TGG + chr8_1:84014828-84014847 None:intergenic 50.0%
GTGACACAACCAACCCCATT+AGG - chr8_1:84014963-84014982 Msa1220730:five_prime_UTR 50.0%
ACTGTTGCTCCTAATGGGGT+TGG + chr8_1:84014975-84014994 None:intergenic 50.0%
CTCAGGATCCCCCATGTTGT+TGG - chr8_1:84012337-84012356 Msa1220730:CDS 55.0%
ATGATGACAGCAGCAGTGGC+AGG + chr8_1:84013017-84013036 None:intergenic 55.0%
CCGAAAACCAGCCAATTCGC+TGG - chr8_1:84013159-84013178 Msa1220730:CDS 55.0%
CAACCAACCAGCGAATTGGC+TGG + chr8_1:84013169-84013188 None:intergenic 55.0%
!! TGTCTCGCCGATTCGATTCC+TGG - chr8_1:84013359-84013378 Msa1220730:intron 55.0%
CTGTGGGTGGAGTGAATGTG+TGG - chr8_1:84014039-84014058 Msa1220730:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 84012096 84015165 84012096 ID=Msa1220730;Name=Msa1220730
chr8_1 mRNA 84012096 84015165 84012096 ID=Msa1220730-mRNA-1;Parent=Msa1220730;Name=Msa1220730-mRNA-1;_AED=0.10;_eAED=0.10;_QI=430|1|1|1|0.5|1|3|138|213
chr8_1 exon 84012096 84012641 84012096 ID=Msa1220730-mRNA-1:exon:30159;Parent=Msa1220730-mRNA-1
chr8_1 exon 84013053 84013288 84013053 ID=Msa1220730-mRNA-1:exon:30158;Parent=Msa1220730-mRNA-1
chr8_1 exon 84014738 84015165 84014738 ID=Msa1220730-mRNA-1:exon:30157;Parent=Msa1220730-mRNA-1
chr8_1 five_prime_UTR 84014738 84015165 84014738 ID=Msa1220730-mRNA-1:five_prime_utr;Parent=Msa1220730-mRNA-1
chr8_1 five_prime_UTR 84013287 84013288 84013287 ID=Msa1220730-mRNA-1:five_prime_utr;Parent=Msa1220730-mRNA-1
chr8_1 CDS 84013053 84013286 84013053 ID=Msa1220730-mRNA-1:cds;Parent=Msa1220730-mRNA-1
chr8_1 CDS 84012234 84012641 84012234 ID=Msa1220730-mRNA-1:cds;Parent=Msa1220730-mRNA-1
chr8_1 three_prime_UTR 84012096 84012233 84012096 ID=Msa1220730-mRNA-1:three_prime_utr;Parent=Msa1220730-mRNA-1
Gene Sequence

>Msa1220730

ATGGGAACCAAAATTGAATGTTCCATAAATCTTGTAGCAACCTTAGTAAAGAGCAATAAGTTAGCTGTGGGTGGAGTGAATGTGTGGGAGTATTATTATCAGAACAAAGGGAGGCTGACCAATAACCATCACAGGACTATAATCAATAAATTGCAAGGTCCCATCATGGATAGAAACAACATAGAATCCATCAAAAAGACAATGCAAATGCATGAGGACATCTTCAAACACCAGGTAAAAGAACTTCACAGAGTATACAGTGTGCAAAAGATGCTGATGAATGAGCTAAAAAATAAAATTAGACAACAAAATTTTTGGAATCCAATGAATGACATAGAAAAACAATTTTCAAGAGGTTTTGATCTTGAAAAGCCAGCTGTGGAAAACACTTTTATTGGATCATCACTTGGCATTGATGAAGGTGAGGTAGGGACCAGTTCCAACACTGCTGCATTTCAGAGCTGTAAATTAAGTACTTTTGATGATTTTAATGAAGAATTGGAAGTAGATTTGACACTAAGCATAGGAGGTAGCCAACTTAAGAATTCTAATATGAAACTTCAATTAGGAGAATGCAGTGACACAACCAACCCCATTAGGAGCAACAGTGTGACATTTACTCAAGGTTTACAGCTTAAATAG
Protein sequence

>Msa1220730

MGTKIECSINLVATLVKSNKLAVGGVNVWEYYYQNKGRLTNNHHRTIINKLQGPIMDRNNIESIKKTMQMHEDIFKHQVKELHRVYSVQKMLMNELKNKIRQQNFWNPMNDIEKQFSRGFDLEKPAVENTFIGSSLGIDEGEVGTSSNTAAFQSCKLSTFDDFNEELEVDLTLSIGGSQLKNSNMKLQLGECSDTTNPIRSNSVTFTQGLQLK*