AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1334660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334660 MtrunA17_Chr4g0072101 96.855 159 5 0 55 213 5 163 6.79e-114 322
Msa1334660 MtrunA17_Chr2g0298071 41.860 258 100 9 1 213 1 253 2.26e-46 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334660 AT5G57340.1 40.164 122 48 5 55 176 57 153 1.94e-14 70.1
Msa1334660 AT5G57340.2 40.164 122 48 5 55 176 75 171 2.31e-14 70.1

Find 57 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGTTGCTACAAGATTTA+TGG 0.154976 8_4:-55135943 None:intergenic
ATTTATGGAACATTCAATTT+TGG 0.200899 8_4:-55135928 None:intergenic
TATAGTCCTGTGATGGTTAT+TGG 0.284417 8_4:-55136039 None:intergenic
TCTAATATGAAACTTCAATT+AGG 0.300464 8_4:+55136878 Msa1334660:CDS
ATTCTGCTACTTCTTATTAC+AGG 0.304234 8_4:+55134450 Msa1334660:five_prime_UTR
TGTCACACTGTTGCTCCTAA+TGG 0.328969 8_4:-55136924 None:intergenic
GTCACACTGTTGCTCCTAAT+GGG 0.332519 8_4:-55136923 None:intergenic
ATTCTCCTTGAGAGACAAAA+AGG 0.342116 8_4:-55134318 None:intergenic
ACTGTGTAAATTAATAATAT+TGG 0.379818 8_4:+55134358 Msa1334660:five_prime_UTR
TTCTATGATATATCTTTCTC+AGG 0.403651 8_4:+55134268 Msa1334660:five_prime_UTR
TAACTTATTGCTCTTTACTA+AGG 0.413153 8_4:-55135961 None:intergenic
AGCAGAATTGCAGCTTTCTC+TGG 0.413595 8_4:-55134435 None:intergenic
TCTTTGTTGTTTCTTAGTGA+AGG 0.423743 8_4:+55134221 Msa1334660:five_prime_UTR
CAAATTTAAAACTATAATCA+TGG 0.423848 8_4:-55134190 None:intergenic
ATTAATAATTCATGCTTGAC+AGG 0.425827 8_4:-55134164 None:intergenic
ATATTCATACTGTAATATAT+AGG 0.433605 8_4:+55134044 Msa1334660:five_prime_UTR
CTTTGTTGTTTCTTAGTGAA+GGG 0.433661 8_4:+55134222 Msa1334660:five_prime_UTR
TATGTTGTTTCTATCCATGA+TGG 0.434780 8_4:-55136081 None:intergenic
GTGACACAACCAACCCCATT+AGG 0.451171 8_4:+55136909 Msa1334660:CDS
ATATTATTAATTTACACAGT+TGG 0.463006 8_4:-55134355 None:intergenic
AATAAAGTAGAAACTAATCT+AGG 0.470222 8_4:-55137062 None:intergenic
ATGTTGTTTCTATCCATGAT+GGG 0.484028 8_4:-55136080 None:intergenic
TCATATTAGAATTCTTAAGT+TGG 0.490514 8_4:-55136865 None:intergenic
AGTATTATTATCAGAACAAA+GGG 0.491849 8_4:+55136009 Msa1334660:CDS
TGTAACCTTTCAACTCTACA+TGG 0.503587 8_4:+55137005 Msa1334660:three_prime_UTR
GCTCTGAAATGCAGCAGTGT+TGG 0.531063 8_4:-55136771 None:intergenic
CTGTGGGTGGAGTGAATGTG+TGG 0.533528 8_4:+55135985 Msa1334660:CDS
AAATGCAGCAGTGTTGGAAC+TGG 0.538023 8_4:-55136765 None:intergenic
ACTGTTGCTCCTAATGGGGT+TGG 0.541229 8_4:-55136918 None:intergenic
TATTGATTATAGTCCTGTGA+TGG 0.542206 8_4:-55136046 None:intergenic
TAAATTGCAAGGTCCCATCA+TGG 0.552827 8_4:+55136067 Msa1334660:CDS
AAATTTAAAACTATAATCAT+GGG 0.553892 8_4:-55134189 None:intergenic
GGCTGACCAATAACCATCAC+AGG 0.553998 8_4:+55136033 Msa1334660:CDS
GTGGTCCATGTAGAGTTGAA+AGG 0.563193 8_4:-55137010 None:intergenic
GGCATTGATGAAGGTGAGGT+AGG 0.564515 8_4:+55136740 Msa1334660:CDS
TCATCACTTGGCATTGATGA+AGG 0.574340 8_4:+55136731 Msa1334660:CDS
ACTATAATCAATAAATTGCA+AGG 0.575944 8_4:+55136056 Msa1334660:CDS
GATTTGACACTAAGCATAGG+AGG 0.578450 8_4:+55136839 Msa1334660:CDS
AAAGAGCAATAAGTTAGCTG+TGG 0.583848 8_4:+55135968 Msa1334660:CDS
GCATTGATGAAGGTGAGGTA+GGG 0.591301 8_4:+55136741 Msa1334660:CDS
TGATCTTGAAAAGCCAGCTG+TGG 0.591394 8_4:+55136691 Msa1334660:CDS
AGCAATAAGTTAGCTGTGGG+TGG 0.591461 8_4:+55135972 Msa1334660:CDS
AACAGTGTGACATTTACTCA+AGG 0.600555 8_4:+55136935 Msa1334660:CDS
AAAGATCAATCCAACAACAT+GGG 0.607629 8_4:-55134295 None:intergenic
AAGATCAATCCAACAACATG+GGG 0.615061 8_4:-55134294 None:intergenic
GAGTATTATTATCAGAACAA+AGG 0.615462 8_4:+55136008 Msa1334660:CDS
GAAAGATCAATCCAACAACA+TGG 0.619463 8_4:-55134296 None:intergenic
AAGAGCAATAAGTTAGCTGT+GGG 0.620145 8_4:+55135969 Msa1334660:CDS
CATGAACCATCAAAATTCAG+TGG 0.623614 8_4:-55137029 None:intergenic
TGTGGGTGGAGTGAATGTGT+GGG 0.624316 8_4:+55135986 Msa1334660:CDS
GTAGATTTGACACTAAGCAT+AGG 0.634592 8_4:+55136836 Msa1334660:CDS
TCACACTGTTGCTCCTAATG+GGG 0.647566 8_4:-55136922 None:intergenic
ATTATTATCAGAACAAAGGG+AGG 0.652018 8_4:+55136012 Msa1334660:CDS
ACTTGGCATTGATGAAGGTG+AGG 0.653122 8_4:+55136736 Msa1334660:CDS
AAAGACAATGCAAATGCATG+AGG 0.654368 8_4:+55136115 Msa1334660:CDS
TGAGGACATCTTCAAACACC+AGG 0.656291 8_4:+55136133 Msa1334660:CDS
AGATCAATCCAACAACATGG+GGG 0.746907 8_4:-55134293 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAAAACTATAATCAT+GGG - chr8_4:55134192-55134211 None:intergenic 10.0%
!!! TAATGGATATAATTATTTTT+TGG + chr8_4:55134552-55134571 Msa1334660:intron 10.0%
!! AAAAAAATAGAAAAAAAAAC+TGG - chr8_4:55135207-55135226 None:intergenic 10.0%
!! ATATTCATACTGTAATATAT+AGG + chr8_4:55134044-55134063 Msa1334660:five_prime_UTR 15.0%
!!! CAAATTTAAAACTATAATCA+TGG - chr8_4:55134193-55134212 None:intergenic 15.0%
!! ATATTATTAATTTACACAGT+TGG - chr8_4:55134358-55134377 None:intergenic 15.0%
!! ACTGTGTAAATTAATAATAT+TGG + chr8_4:55134358-55134377 Msa1334660:five_prime_UTR 15.0%
!! ATAATTATATCCATTACATT+AGG - chr8_4:55134548-55134567 None:intergenic 15.0%
!!! TGAACAAATGTTTTAAAAAT+CGG - chr8_4:55135333-55135352 None:intergenic 15.0%
!!! TGGTTTTAATTTTTGTATTT+AGG + chr8_4:55135897-55135916 Msa1334660:intron 15.0%
!!! TTAATTTTTGTATTTAGGTA+TGG + chr8_4:55135902-55135921 Msa1334660:intron 15.0%
!!! TAATTTTTGTATTTAGGTAT+GGG + chr8_4:55135903-55135922 Msa1334660:intron 15.0%
!!! TTAACTTGAATTATGTATTT+GGG - chr8_4:55136196-55136215 None:intergenic 15.0%
!!! ATTAACTTGAATTATGTATT+TGG - chr8_4:55136197-55136216 None:intergenic 15.0%
!!! ATTTTCAAGTTTACTAAAAT+TGG + chr8_4:55136317-55136336 Msa1334660:intron 15.0%
!!! AAATTAGACAACAAAATTTT+TGG + chr8_4:55136627-55136646 Msa1334660:CDS 15.0%
!!! GTATAACTAATTCAAGTTAA+TGG - chr8_4:55134522-55134541 None:intergenic 20.0%
!!! ATTATTTTTTGGTGTTTATG+TGG + chr8_4:55134563-55134582 Msa1334660:intron 20.0%
!!! TTATTTTTTATGTATCACCA+TGG - chr8_4:55134655-55134674 None:intergenic 20.0%
!! AGTGAAAAAATTTATGAGAT+GGG - chr8_4:55134836-55134855 None:intergenic 20.0%
!! TAGTGAAAAAATTTATGAGA+TGG - chr8_4:55134837-55134856 None:intergenic 20.0%
!! GTATTTATTTATTTGTCATC+CGG + chr8_4:55135239-55135258 Msa1334660:intron 20.0%
!!! CTTATTTTCATAAGTTTTTC+AGG + chr8_4:55135766-55135785 Msa1334660:intron 20.0%
!!! TTTATTTTCATGCTGTTTAT+AGG - chr8_4:55135796-55135815 None:intergenic 20.0%
!!! ATTTATGGAACATTCAATTT+TGG - chr8_4:55135931-55135950 None:intergenic 20.0%
!! AGTATTATTATCAGAACAAA+GGG + chr8_4:55136009-55136028 Msa1334660:CDS 20.0%
!! ACTATAATCAATAAATTGCA+AGG + chr8_4:55136056-55136075 Msa1334660:CDS 20.0%
!! AAAAGTAAAACCATAGTAAT+TGG + chr8_4:55136255-55136274 Msa1334660:intron 20.0%
!!! AATTTTAATCTCTACTAGTT+AGG + chr8_4:55136518-55136537 Msa1334660:intron 20.0%
!!! ATAGAAAAACAATTTTCAAG+AGG + chr8_4:55136665-55136684 Msa1334660:CDS 20.0%
!!! TGATGATTTTAATGAAGAAT+TGG + chr8_4:55136811-55136830 Msa1334660:CDS 20.0%
!! TCATATTAGAATTCTTAAGT+TGG - chr8_4:55136868-55136887 None:intergenic 20.0%
!! TCTAATATGAAACTTCAATT+AGG + chr8_4:55136878-55136897 Msa1334660:CDS 20.0%
!! AATAAAGTAGAAACTAATCT+AGG - chr8_4:55137065-55137084 None:intergenic 20.0%
! ATTAATAATTCATGCTTGAC+AGG - chr8_4:55134167-55134186 None:intergenic 25.0%
! TTCTATGATATATCTTTCTC+AGG + chr8_4:55134268-55134287 Msa1334660:five_prime_UTR 25.0%
! GTTATACAATCCTAATGTAA+TGG + chr8_4:55134535-55134554 Msa1334660:intron 25.0%
! GAGTTTACAATTAAAAGTCA+AGG - chr8_4:55134718-55134737 None:intergenic 25.0%
! AAATTATTGAGCTATGTAGA+AGG - chr8_4:55134767-55134786 None:intergenic 25.0%
! TGAATTAAGTACTACTGTTA+AGG + chr8_4:55134920-55134939 Msa1334660:intron 25.0%
! GAATTAAGTACTACTGTTAA+GGG + chr8_4:55134921-55134940 Msa1334660:intron 25.0%
! GTAACATGAACAATAGATTT+AGG - chr8_4:55135021-55135040 None:intergenic 25.0%
! ATTGACATGAAAATTGAAGT+AGG - chr8_4:55135046-55135065 None:intergenic 25.0%
! CAATTCAATACAAATTCTCT+TGG + chr8_4:55135372-55135391 Msa1334660:intron 25.0%
! AACTGAAACAAAAATCCAAA+TGG - chr8_4:55135573-55135592 None:intergenic 25.0%
! GAAACTAGAAACCAAAATTT+AGG - chr8_4:55135659-55135678 None:intergenic 25.0%
! TAACTTATTGCTCTTTACTA+AGG - chr8_4:55135964-55135983 None:intergenic 25.0%
! GAGTATTATTATCAGAACAA+AGG + chr8_4:55136008-55136027 Msa1334660:CDS 25.0%
! TTCATGTAATGTAACATTTG+TGG - chr8_4:55136237-55136256 None:intergenic 25.0%
!! ACAAATTTTCGTTTACTACA+AGG + chr8_4:55136351-55136370 Msa1334660:intron 25.0%
!!! TAACCCTTATTGATTTTGAA+AGG + chr8_4:55136478-55136497 Msa1334660:intron 25.0%
! TGTCCTTTCAAAATCAATAA+GGG - chr8_4:55136484-55136503 None:intergenic 25.0%
! ATGTCCTTTCAAAATCAATA+AGG - chr8_4:55136485-55136504 None:intergenic 25.0%
!! AGTTAGGAATCATATGTTTT+TGG + chr8_4:55136534-55136553 Msa1334660:intron 25.0%
!! TGTTTTTCTATGTCATTCAT+TGG - chr8_4:55136656-55136675 None:intergenic 25.0%
!! TCTTTGTTGTTTCTTAGTGA+AGG + chr8_4:55134221-55134240 Msa1334660:five_prime_UTR 30.0%
!! CTTTGTTGTTTCTTAGTGAA+GGG + chr8_4:55134222-55134241 Msa1334660:five_prime_UTR 30.0%
AAAGATCAATCCAACAACAT+GGG - chr8_4:55134298-55134317 None:intergenic 30.0%
ATTGGAGTTGAGTGTAAAAA+TGG + chr8_4:55134376-55134395 Msa1334660:five_prime_UTR 30.0%
! ATTCTGCTACTTCTTATTAC+AGG + chr8_4:55134450-55134469 Msa1334660:five_prime_UTR 30.0%
AATATGTATCTGTGAAGTGT+AGG - chr8_4:55134494-55134513 None:intergenic 30.0%
TTTAATGCTTCTTTAGTCCA+TGG + chr8_4:55134635-55134654 Msa1334660:intron 30.0%
ACATAAACTTAGACACTATG+AGG - chr8_4:55134740-55134759 None:intergenic 30.0%
TACTACTGTTAAGGGATTTA+TGG + chr8_4:55134929-55134948 Msa1334660:intron 30.0%
TAACAATGAAACCAACATGT+TGG - chr8_4:55134998-55135017 None:intergenic 30.0%
!!! TGTTTTAATTGGTTCAACCA+TGG - chr8_4:55135270-55135289 None:intergenic 30.0%
GAACCAATTAAAACATGACT+TGG + chr8_4:55135275-55135294 Msa1334660:intron 30.0%
!!! TCTCCAAGTCATGTTTTAAT+TGG - chr8_4:55135281-55135300 None:intergenic 30.0%
!!! AATGTTTTAAAAATCGGTCC+AGG - chr8_4:55135327-55135346 None:intergenic 30.0%
!! TCCACTTTTGGTTATTAAAG+TGG + chr8_4:55135414-55135433 Msa1334660:intron 30.0%
TCCACTTTAATAACCAAAAG+TGG - chr8_4:55135418-55135437 None:intergenic 30.0%
! AATTGAAACAGGCAACTTTT+AGG - chr8_4:55135494-55135513 None:intergenic 30.0%
TTTGTTTCAGTTTCTTGCTA+TGG + chr8_4:55135581-55135600 Msa1334660:intron 30.0%
TTTACCTAGTTTGATCTCAA+CGG + chr8_4:55135631-55135650 Msa1334660:intron 30.0%
GTTGAAAACAGTCTATAGAT+AGG - chr8_4:55135747-55135766 None:intergenic 30.0%
AACCATTGCTAATCATGTAA+GGG - chr8_4:55135882-55135901 None:intergenic 30.0%
AAACCATTGCTAATCATGTA+AGG - chr8_4:55135883-55135902 None:intergenic 30.0%
TAAGGTTGCTACAAGATTTA+TGG - chr8_4:55135946-55135965 None:intergenic 30.0%
ATTATTATCAGAACAAAGGG+AGG + chr8_4:55136012-55136031 Msa1334660:CDS 30.0%
! TATTGATTATAGTCCTGTGA+TGG - chr8_4:55136049-55136068 None:intergenic 30.0%
!! ATGTTGTTTCTATCCATGAT+GGG - chr8_4:55136083-55136102 None:intergenic 30.0%
!! TATGTTGTTTCTATCCATGA+TGG - chr8_4:55136084-55136103 None:intergenic 30.0%
! CATTTGCATTGTCTTTTTGA+TGG - chr8_4:55136111-55136130 None:intergenic 30.0%
TTCAGTGATATGAAATCTTC+AGG - chr8_4:55136162-55136181 None:intergenic 30.0%
GTAAAACCATAGTAATTGGT+AGG + chr8_4:55136259-55136278 Msa1334660:intron 30.0%
TATAAGCCTACCAATTACTA+TGG - chr8_4:55136268-55136287 None:intergenic 30.0%
!!! CATATGTTTTTGGAATTTGC+AGG + chr8_4:55136544-55136563 Msa1334660:intron 30.0%
! ACTTTTATTGGATCATCACT+TGG + chr8_4:55136719-55136738 Msa1334660:CDS 30.0%
AAGATCAATCCAACAACATG+GGG - chr8_4:55134297-55134316 None:intergenic 35.0%
GAAAGATCAATCCAACAACA+TGG - chr8_4:55134299-55134318 None:intergenic 35.0%
! TCTTTCCTTTTTGTCTCTCA+AGG + chr8_4:55134313-55134332 Msa1334660:five_prime_UTR 35.0%
ATTCTCCTTGAGAGACAAAA+AGG - chr8_4:55134321-55134340 None:intergenic 35.0%
GTGGAGTTTCCAAACATATA+TGG + chr8_4:55134582-55134601 Msa1334660:intron 35.0%
ACACATGCACCATATATGTT+TGG - chr8_4:55134594-55134613 None:intergenic 35.0%
TGATTCCCCCACATAAATTT+AGG + chr8_4:55134857-55134876 Msa1334660:intron 35.0%
ACTTAATTCATCTGCAACTC+TGG - chr8_4:55134910-55134929 None:intergenic 35.0%
ATAATCACAGACCAACATGT+TGG + chr8_4:55134984-55135003 Msa1334660:intron 35.0%
AGAACTCTCGATTGAACTAT+AGG + chr8_4:55135148-55135167 Msa1334660:intron 35.0%
!! GAACTATAGGTTGATTGAAC+CGG + chr8_4:55135161-55135180 Msa1334660:intron 35.0%
CCTGTTTCAATTCACCAAAA+GGG + chr8_4:55135502-55135521 Msa1334660:intron 35.0%
TAATGTCAGCTTATCATGAC+TGG + chr8_4:55135546-55135565 Msa1334660:intron 35.0%
! TCTCAACGGTTCCTAAATTT+TGG + chr8_4:55135645-55135664 Msa1334660:intron 35.0%
TCAAGCTACAATGTGAAGAT+GGG - chr8_4:55135714-55135733 None:intergenic 35.0%
TACCCTTACATGATTAGCAA+TGG + chr8_4:55135877-55135896 Msa1334660:intron 35.0%
AAAGAGCAATAAGTTAGCTG+TGG + chr8_4:55135968-55135987 Msa1334660:CDS 35.0%
AAGAGCAATAAGTTAGCTGT+GGG + chr8_4:55135969-55135988 Msa1334660:CDS 35.0%
TATAGTCCTGTGATGGTTAT+TGG - chr8_4:55136042-55136061 None:intergenic 35.0%
AAAGACAATGCAAATGCATG+AGG + chr8_4:55136115-55136134 Msa1334660:CDS 35.0%
TATGAAATCTTCAGGATACC+TGG - chr8_4:55136154-55136173 None:intergenic 35.0%
CATTTGTGGCACAGATTAAA+TGG - chr8_4:55136223-55136242 None:intergenic 35.0%
!!! ATAAAAGTGTTTTCCACAGC+TGG - chr8_4:55136707-55136726 None:intergenic 35.0%
! GCTGTGGAAAACACTTTTAT+TGG + chr8_4:55136707-55136726 Msa1334660:CDS 35.0%
GTAGATTTGACACTAAGCAT+AGG + chr8_4:55136836-55136855 Msa1334660:CDS 35.0%
AACAGTGTGACATTTACTCA+AGG + chr8_4:55136935-55136954 Msa1334660:CDS 35.0%
TGTAACCTTTCAACTCTACA+TGG + chr8_4:55137005-55137024 Msa1334660:three_prime_UTR 35.0%
CATGAACCATCAAAATTCAG+TGG - chr8_4:55137032-55137051 None:intergenic 35.0%
AGGCTGTTGCAAGAAAAAGA+TGG - chr8_4:55134147-55134166 None:intergenic 40.0%
AGATCAATCCAACAACATGG+GGG - chr8_4:55134296-55134315 None:intergenic 40.0%
GGAGTTGAGTGTAAAAATGG+TGG + chr8_4:55134379-55134398 Msa1334660:five_prime_UTR 40.0%
AGTGTAAAAATGGTGGCATG+AGG + chr8_4:55134386-55134405 Msa1334660:five_prime_UTR 40.0%
GTGTAAAAATGGTGGCATGA+GGG + chr8_4:55134387-55134406 Msa1334660:five_prime_UTR 40.0%
TGAGGCCTAAATTTATGTGG+GGG - chr8_4:55134865-55134884 None:intergenic 40.0%
GTGAGGCCTAAATTTATGTG+GGG - chr8_4:55134866-55134885 None:intergenic 40.0%
GGTGAGGCCTAAATTTATGT+GGG - chr8_4:55134867-55134886 None:intergenic 40.0%
AGGTGAGGCCTAAATTTATG+TGG - chr8_4:55134868-55134887 None:intergenic 40.0%
AGAAAATCCAACACAAGGTG+AGG - chr8_4:55134883-55134902 None:intergenic 40.0%
! ACCTTGTGTTGGATTTTCTG+AGG + chr8_4:55134884-55134903 Msa1334660:intron 40.0%
TTATATGATGACAGCAGCAG+TGG - chr8_4:55134968-55134987 None:intergenic 40.0%
! TCTCTATCTCTCTCCACTTT+TGG + chr8_4:55135402-55135421 Msa1334660:intron 40.0%
!! GCAACTTTTAGGAAGGTTTC+TGG - chr8_4:55135483-55135502 None:intergenic 40.0%
!! GAAACAGGCAACTTTTAGGA+AGG - chr8_4:55135490-55135509 None:intergenic 40.0%
GCCTGTTTCAATTCACCAAA+AGG + chr8_4:55135501-55135520 Msa1334660:intron 40.0%
! CCCTTTTGGTGAATTGAAAC+AGG - chr8_4:55135505-55135524 None:intergenic 40.0%
! AGATACTGTAACTGCCCTTT+TGG - chr8_4:55135519-55135538 None:intergenic 40.0%
! CTTATCATGACTGGTCCATT+TGG + chr8_4:55135555-55135574 Msa1334660:intron 40.0%
!! TCTTGCTATGGTACTGATTG+TGG + chr8_4:55135593-55135612 Msa1334660:intron 40.0%
CAAGCTACAATGTGAAGATG+GGG - chr8_4:55135713-55135732 None:intergenic 40.0%
CTCAAGCTACAATGTGAAGA+TGG - chr8_4:55135715-55135734 None:intergenic 40.0%
TCTTCACATTGTAGCTTGAG+AGG + chr8_4:55135715-55135734 Msa1334660:intron 40.0%
TAAATTGCAAGGTCCCATCA+TGG + chr8_4:55136067-55136086 Msa1334660:CDS 40.0%
!! TCATCACTTGGCATTGATGA+AGG + chr8_4:55136731-55136750 Msa1334660:CDS 40.0%
GATTTGACACTAAGCATAGG+AGG + chr8_4:55136839-55136858 Msa1334660:CDS 40.0%
! CATGGACCACTGAATTTTGA+TGG + chr8_4:55137023-55137042 Msa1334660:three_prime_UTR 40.0%
TAAAAATGGTGGCATGAGGG+AGG + chr8_4:55134390-55134409 Msa1334660:five_prime_UTR 45.0%
AGCAGAATTGCAGCTTTCTC+TGG - chr8_4:55134438-55134457 None:intergenic 45.0%
ATTTAGGCCTCACCTTGTGT+TGG + chr8_4:55134873-55134892 Msa1334660:intron 45.0%
GCCTCAGAAAATCCAACACA+AGG - chr8_4:55134888-55134907 None:intergenic 45.0%
ATTGGTCGAACTGGCTGATT+CGG - chr8_4:55135082-55135101 None:intergenic 45.0%
TTCGGTTCAATTGGTCGAAC+TGG - chr8_4:55135091-55135110 None:intergenic 45.0%
!!! TGGCTGGTTTTCGGTTCAAT+TGG - chr8_4:55135100-55135119 None:intergenic 45.0%
TGAACCATCTGCTGTTGAAC+CGG - chr8_4:55135183-55135202 None:intergenic 45.0%
TTTGTCATCCGGTTCAACCA+TGG + chr8_4:55135250-55135269 Msa1334660:intron 45.0%
TGGTTCAACCATGGTTGAAC+CGG - chr8_4:55135261-55135280 None:intergenic 45.0%
AATCGGTCCAGGAATCGAAT+CGG - chr8_4:55135316-55135335 None:intergenic 45.0%
AGTGGAGAGAGATAGAGAGA+GGG - chr8_4:55135400-55135419 None:intergenic 45.0%
AAGTGGAGAGAGATAGAGAG+AGG - chr8_4:55135401-55135420 None:intergenic 45.0%
!! ATGGTACTGATTGTGGTCTC+AGG + chr8_4:55135600-55135619 Msa1334660:intron 45.0%
GGAACCGTTGAGATCAAACT+AGG - chr8_4:55135638-55135657 None:intergenic 45.0%
AGCAATAAGTTAGCTGTGGG+TGG + chr8_4:55135972-55135991 Msa1334660:CDS 45.0%
TGAGGACATCTTCAAACACC+AGG + chr8_4:55136133-55136152 Msa1334660:CDS 45.0%
TGATCTTGAAAAGCCAGCTG+TGG + chr8_4:55136691-55136710 Msa1334660:CDS 45.0%
!! ACTTGGCATTGATGAAGGTG+AGG + chr8_4:55136736-55136755 Msa1334660:CDS 45.0%
! GCATTGATGAAGGTGAGGTA+GGG + chr8_4:55136741-55136760 Msa1334660:CDS 45.0%
! AAATGCAGCAGTGTTGGAAC+TGG - chr8_4:55136768-55136787 None:intergenic 45.0%
TCACACTGTTGCTCCTAATG+GGG - chr8_4:55136925-55136944 None:intergenic 45.0%
GTCACACTGTTGCTCCTAAT+GGG - chr8_4:55136926-55136945 None:intergenic 45.0%
TGTCACACTGTTGCTCCTAA+TGG - chr8_4:55136927-55136946 None:intergenic 45.0%
GTGGTCCATGTAGAGTTGAA+AGG - chr8_4:55137013-55137032 None:intergenic 45.0%
!! ATAAAAAAAGTAAAAAAATT+AGG + chr8_4:55135811-55135830 Msa1334660:intron 5.0%
!!! CCAGCGAATTGGCTGGTTTT+CGG - chr8_4:55135109-55135128 None:intergenic 50.0%
AAACCAGCCAATTCGCTGGT+TGG + chr8_4:55135110-55135129 Msa1334660:intron 50.0%
CCAACAACCAACCAGCGAAT+TGG - chr8_4:55135120-55135139 None:intergenic 50.0%
! CCAATTCGCTGGTTGGTTGT+TGG + chr8_4:55135117-55135136 Msa1334660:intron 50.0%
! TGAACCGGTTCAACAGCAGA+TGG + chr8_4:55135176-55135195 Msa1334660:intron 50.0%
TGGAGAGAGATAGAGAGAGG+GGG - chr8_4:55135398-55135417 None:intergenic 50.0%
GTGGAGAGAGATAGAGAGAG+GGG - chr8_4:55135399-55135418 None:intergenic 50.0%
TGTGGGTGGAGTGAATGTGT+GGG + chr8_4:55135986-55136005 Msa1334660:CDS 50.0%
GGCTGACCAATAACCATCAC+AGG + chr8_4:55136033-55136052 Msa1334660:CDS 50.0%
!! GGCATTGATGAAGGTGAGGT+AGG + chr8_4:55136740-55136759 Msa1334660:CDS 50.0%
!! GCTCTGAAATGCAGCAGTGT+TGG - chr8_4:55136774-55136793 None:intergenic 50.0%
GTGACACAACCAACCCCATT+AGG + chr8_4:55136909-55136928 Msa1334660:CDS 50.0%
ACTGTTGCTCCTAATGGGGT+TGG - chr8_4:55136921-55136940 None:intergenic 50.0%
CTCAGGATCCCCCATGTTGT+TGG + chr8_4:55134285-55134304 Msa1334660:five_prime_UTR 55.0%
ATGATGACAGCAGCAGTGGC+AGG - chr8_4:55134964-55134983 None:intergenic 55.0%
CCGAAAACCAGCCAATTCGC+TGG + chr8_4:55135106-55135125 Msa1334660:intron 55.0%
CAACCAACCAGCGAATTGGC+TGG - chr8_4:55135116-55135135 None:intergenic 55.0%
!! TGTCTCGCCGATTCGATTCC+TGG + chr8_4:55135306-55135325 Msa1334660:intron 55.0%
CTGTGGGTGGAGTGAATGTG+TGG + chr8_4:55135985-55136004 Msa1334660:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 55134044 55137111 55134044 ID=Msa1334660;Name=Msa1334660
chr8_4 mRNA 55134044 55137111 55134044 ID=Msa1334660-mRNA-1;Parent=Msa1334660;Name=Msa1334660-mRNA-1;_AED=0.10;_eAED=0.10;_QI=430|1|1|1|0.5|1|3|138|213
chr8_4 exon 55134044 55134471 55134044 ID=Msa1334660-mRNA-1:exon:17195;Parent=Msa1334660-mRNA-1
chr8_4 exon 55135919 55136154 55135919 ID=Msa1334660-mRNA-1:exon:17196;Parent=Msa1334660-mRNA-1
chr8_4 exon 55136566 55137111 55136566 ID=Msa1334660-mRNA-1:exon:17197;Parent=Msa1334660-mRNA-1
chr8_4 five_prime_UTR 55134044 55134471 55134044 ID=Msa1334660-mRNA-1:five_prime_utr;Parent=Msa1334660-mRNA-1
chr8_4 five_prime_UTR 55135919 55135920 55135919 ID=Msa1334660-mRNA-1:five_prime_utr;Parent=Msa1334660-mRNA-1
chr8_4 CDS 55135921 55136154 55135921 ID=Msa1334660-mRNA-1:cds;Parent=Msa1334660-mRNA-1
chr8_4 CDS 55136566 55136973 55136566 ID=Msa1334660-mRNA-1:cds;Parent=Msa1334660-mRNA-1
chr8_4 three_prime_UTR 55136974 55137111 55136974 ID=Msa1334660-mRNA-1:three_prime_utr;Parent=Msa1334660-mRNA-1
Gene Sequence

>Msa1334660

ATGGGAACCAAAATTGAATGTTCCATAAATCTTGTAGCAACCTTAGTAAAGAGCAATAAGTTAGCTGTGGGTGGAGTGAATGTGTGGGAGTATTATTATCAGAACAAAGGGAGGCTGACCAATAACCATCACAGGACTATAATCAATAAATTGCAAGGTCCCATCATGGATAGAAACAACATAGAATCCATCAAAAAGACAATGCAAATGCATGAGGACATCTTCAAACACCAGGTAAAAGAACTTCACAGAGTATACAGTGTGCAAAAGATGCTGATGAATGAGCTAAAAAATAAAATTAGACAACAAAATTTTTGGAATCCAATGAATGACATAGAAAAACAATTTTCAAGAGGTTTTGATCTTGAAAAGCCAGCTGTGGAAAACACTTTTATTGGATCATCACTTGGCATTGATGAAGGTGAGGTAGGGACCAGTTCCAACACTGCTGCATTTCAGAGCTGTAAATTAAGTACTTTTGATGATTTTAATGAAGAATTGGAAGTAGATTTGACACTAAGCATAGGAGGTAGCCAACTTAAGAATTCTAATATGAAACTTCAATTAGGAGAATGCAGTGACACAACCAACCCCATTAGGAGCAACAGTGTGACATTTACTCAAGGTTTACAGCTTAAATAG
Protein sequence

>Msa1334660

MGTKIECSINLVATLVKSNKLAVGGVNVWEYYYQNKGRLTNNHHRTIINKLQGPIMDRNNIESIKKTMQMHEDIFKHQVKELHRVYSVQKMLMNELKNKIRQQNFWNPMNDIEKQFSRGFDLEKPAVENTFIGSSLGIDEGEVGTSSNTAAFQSCKLSTFDDFNEELEVDLTLSIGGSQLKNSNMKLQLGECSDTTNPIRSNSVTFTQGLQLK*