AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1265560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265560 MtrunA17_Chr4g0072101 96.855 159 5 0 55 213 5 163 6.79e-114 322
Msa1265560 MtrunA17_Chr2g0298071 41.860 258 100 9 1 213 1 253 2.26e-46 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265560 AT5G57340.1 40.164 122 48 5 55 176 57 153 1.94e-14 70.1
Msa1265560 AT5G57340.2 40.164 122 48 5 55 176 75 171 2.31e-14 70.1

Find 57 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGTTGCTACAAGATTTA+TGG 0.154976 8_2:-69960244 None:intergenic
ATTTATGGAACATTCAATTT+TGG 0.200899 8_2:-69960229 None:intergenic
TATAGTCCTGTGATGGTTAT+TGG 0.284417 8_2:-69960340 None:intergenic
TCTAATATGAAACTTCAATT+AGG 0.300464 8_2:+69961179 Msa1265560:CDS
ATTCTGCTACTTCTTATTAC+AGG 0.304234 8_2:+69958749 Msa1265560:five_prime_UTR
TGTCACACTGTTGCTCCTAA+TGG 0.328969 8_2:-69961225 None:intergenic
GTCACACTGTTGCTCCTAAT+GGG 0.332519 8_2:-69961224 None:intergenic
ATTCTCCTTGAGAGACAAAA+AGG 0.342116 8_2:-69958617 None:intergenic
ACTGTGTAAATTAATAATAT+TGG 0.379818 8_2:+69958657 Msa1265560:five_prime_UTR
TTCTATGATATATCTTTCTC+AGG 0.403651 8_2:+69958567 Msa1265560:five_prime_UTR
TAACTTATTGCTCTTTACTA+AGG 0.413153 8_2:-69960262 None:intergenic
AGCAGAATTGCAGCTTTCTC+TGG 0.413595 8_2:-69958734 None:intergenic
TCTTTGTTGTTTCTTAGTGA+AGG 0.423743 8_2:+69958520 Msa1265560:five_prime_UTR
CAAATTTAAAACTATAATCA+TGG 0.423848 8_2:-69958489 None:intergenic
ATTAATAATTCATGCTTGAC+AGG 0.425827 8_2:-69958463 None:intergenic
ATATTCATACTGTAATATAT+AGG 0.433605 8_2:+69958343 Msa1265560:five_prime_UTR
CTTTGTTGTTTCTTAGTGAA+GGG 0.433661 8_2:+69958521 Msa1265560:five_prime_UTR
TATGTTGTTTCTATCCATGA+TGG 0.434780 8_2:-69960382 None:intergenic
GTGACACAACCAACCCCATT+AGG 0.451171 8_2:+69961210 Msa1265560:CDS
ATATTATTAATTTACACAGT+TGG 0.463006 8_2:-69958654 None:intergenic
AATAAAGTAGAAACTAATCT+AGG 0.470222 8_2:-69961363 None:intergenic
ATGTTGTTTCTATCCATGAT+GGG 0.484028 8_2:-69960381 None:intergenic
TCATATTAGAATTCTTAAGT+TGG 0.490514 8_2:-69961166 None:intergenic
AGTATTATTATCAGAACAAA+GGG 0.491849 8_2:+69960310 Msa1265560:CDS
TGTAACCTTTCAACTCTACA+TGG 0.503587 8_2:+69961306 Msa1265560:three_prime_UTR
GCTCTGAAATGCAGCAGTGT+TGG 0.531063 8_2:-69961072 None:intergenic
CTGTGGGTGGAGTGAATGTG+TGG 0.533528 8_2:+69960286 Msa1265560:CDS
AAATGCAGCAGTGTTGGAAC+TGG 0.538023 8_2:-69961066 None:intergenic
ACTGTTGCTCCTAATGGGGT+TGG 0.541229 8_2:-69961219 None:intergenic
TATTGATTATAGTCCTGTGA+TGG 0.542206 8_2:-69960347 None:intergenic
TAAATTGCAAGGTCCCATCA+TGG 0.552827 8_2:+69960368 Msa1265560:CDS
AAATTTAAAACTATAATCAT+GGG 0.553892 8_2:-69958488 None:intergenic
GGCTGACCAATAACCATCAC+AGG 0.553998 8_2:+69960334 Msa1265560:CDS
GTGGTCCATGTAGAGTTGAA+AGG 0.563193 8_2:-69961311 None:intergenic
GGCATTGATGAAGGTGAGGT+AGG 0.564515 8_2:+69961041 Msa1265560:CDS
TCATCACTTGGCATTGATGA+AGG 0.574340 8_2:+69961032 Msa1265560:CDS
ACTATAATCAATAAATTGCA+AGG 0.575944 8_2:+69960357 Msa1265560:CDS
GATTTGACACTAAGCATAGG+AGG 0.578450 8_2:+69961140 Msa1265560:CDS
AAAGAGCAATAAGTTAGCTG+TGG 0.583848 8_2:+69960269 Msa1265560:CDS
GCATTGATGAAGGTGAGGTA+GGG 0.591301 8_2:+69961042 Msa1265560:CDS
TGATCTTGAAAAGCCAGCTG+TGG 0.591394 8_2:+69960992 Msa1265560:CDS
AGCAATAAGTTAGCTGTGGG+TGG 0.591461 8_2:+69960273 Msa1265560:CDS
AACAGTGTGACATTTACTCA+AGG 0.600555 8_2:+69961236 Msa1265560:CDS
AAAGATCAATCCAACAACAT+GGG 0.607629 8_2:-69958594 None:intergenic
AAGATCAATCCAACAACATG+GGG 0.615061 8_2:-69958593 None:intergenic
GAGTATTATTATCAGAACAA+AGG 0.615462 8_2:+69960309 Msa1265560:CDS
GAAAGATCAATCCAACAACA+TGG 0.619463 8_2:-69958595 None:intergenic
AAGAGCAATAAGTTAGCTGT+GGG 0.620145 8_2:+69960270 Msa1265560:CDS
CATGAACCATCAAAATTCAG+TGG 0.623614 8_2:-69961330 None:intergenic
TGTGGGTGGAGTGAATGTGT+GGG 0.624316 8_2:+69960287 Msa1265560:CDS
GTAGATTTGACACTAAGCAT+AGG 0.634592 8_2:+69961137 Msa1265560:CDS
TCACACTGTTGCTCCTAATG+GGG 0.647566 8_2:-69961223 None:intergenic
ATTATTATCAGAACAAAGGG+AGG 0.652018 8_2:+69960313 Msa1265560:CDS
ACTTGGCATTGATGAAGGTG+AGG 0.653122 8_2:+69961037 Msa1265560:CDS
AAAGACAATGCAAATGCATG+AGG 0.654368 8_2:+69960416 Msa1265560:CDS
TGAGGACATCTTCAAACACC+AGG 0.656291 8_2:+69960434 Msa1265560:CDS
AGATCAATCCAACAACATGG+GGG 0.746907 8_2:-69958592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAAAACTATAATCAT+GGG - chr8_2:69958491-69958510 None:intergenic 10.0%
!!! TAATGGATATAATTATTTTT+TGG + chr8_2:69958851-69958870 Msa1265560:intron 10.0%
!! AAAAAAATAGAAAAAAAAAC+TGG - chr8_2:69959507-69959526 None:intergenic 10.0%
!! ATATTCATACTGTAATATAT+AGG + chr8_2:69958343-69958362 Msa1265560:five_prime_UTR 15.0%
!!! CAAATTTAAAACTATAATCA+TGG - chr8_2:69958492-69958511 None:intergenic 15.0%
!! ATATTATTAATTTACACAGT+TGG - chr8_2:69958657-69958676 None:intergenic 15.0%
!! ACTGTGTAAATTAATAATAT+TGG + chr8_2:69958657-69958676 Msa1265560:five_prime_UTR 15.0%
!! ATAATTATATCCATTACATT+AGG - chr8_2:69958847-69958866 None:intergenic 15.0%
!!! TGAACAAATGTTTTAAAAAT+CGG - chr8_2:69959633-69959652 None:intergenic 15.0%
!!! TGGTTTTAATTTTTGTATTT+AGG + chr8_2:69960198-69960217 Msa1265560:intron 15.0%
!!! TTAATTTTTGTATTTAGGTA+TGG + chr8_2:69960203-69960222 Msa1265560:intron 15.0%
!!! TAATTTTTGTATTTAGGTAT+GGG + chr8_2:69960204-69960223 Msa1265560:intron 15.0%
!!! TTAACTTGAATTATGTATTT+GGG - chr8_2:69960497-69960516 None:intergenic 15.0%
!!! ATTAACTTGAATTATGTATT+TGG - chr8_2:69960498-69960517 None:intergenic 15.0%
!!! ATTTTCAAGTTTACTAAAAT+TGG + chr8_2:69960618-69960637 Msa1265560:intron 15.0%
!!! AAATTAGACAACAAAATTTT+TGG + chr8_2:69960928-69960947 Msa1265560:CDS 15.0%
!!! GTATAACTAATTCAAGTTAA+TGG - chr8_2:69958821-69958840 None:intergenic 20.0%
!!! ATTATTTTTTGGTGTTTATG+TGG + chr8_2:69958862-69958881 Msa1265560:intron 20.0%
!!! TTATTTTTTATGTATCACCA+TGG - chr8_2:69958954-69958973 None:intergenic 20.0%
!!! AGTGAAAAATTTTATGAGAT+GGG - chr8_2:69959136-69959155 None:intergenic 20.0%
!!! TAGTGAAAAATTTTATGAGA+TGG - chr8_2:69959137-69959156 None:intergenic 20.0%
!! GTATTTATTTATTTGTCATC+CGG + chr8_2:69959539-69959558 Msa1265560:intron 20.0%
!!! CTTATTTTCATAAGTTTTTC+AGG + chr8_2:69960067-69960086 Msa1265560:intron 20.0%
!!! TTTATTTTCATGCTGTTTAT+AGG - chr8_2:69960097-69960116 None:intergenic 20.0%
!!! ATTTATGGAACATTCAATTT+TGG - chr8_2:69960232-69960251 None:intergenic 20.0%
!! AGTATTATTATCAGAACAAA+GGG + chr8_2:69960310-69960329 Msa1265560:CDS 20.0%
!! ACTATAATCAATAAATTGCA+AGG + chr8_2:69960357-69960376 Msa1265560:CDS 20.0%
!! AAAAGTAAAACCATAGTAAT+TGG + chr8_2:69960556-69960575 Msa1265560:intron 20.0%
!!! AATTTTAATCTCTACTAGTT+AGG + chr8_2:69960819-69960838 Msa1265560:intron 20.0%
!!! ATAGAAAAACAATTTTCAAG+AGG + chr8_2:69960966-69960985 Msa1265560:CDS 20.0%
!!! TGATGATTTTAATGAAGAAT+TGG + chr8_2:69961112-69961131 Msa1265560:CDS 20.0%
!! TCATATTAGAATTCTTAAGT+TGG - chr8_2:69961169-69961188 None:intergenic 20.0%
!! TCTAATATGAAACTTCAATT+AGG + chr8_2:69961179-69961198 Msa1265560:CDS 20.0%
!! AATAAAGTAGAAACTAATCT+AGG - chr8_2:69961366-69961385 None:intergenic 20.0%
! ATTAATAATTCATGCTTGAC+AGG - chr8_2:69958466-69958485 None:intergenic 25.0%
! TTCTATGATATATCTTTCTC+AGG + chr8_2:69958567-69958586 Msa1265560:five_prime_UTR 25.0%
! GTTATACAATCCTAATGTAA+TGG + chr8_2:69958834-69958853 Msa1265560:intron 25.0%
! GAGTTTACAATTAAAAGTCA+AGG - chr8_2:69959017-69959036 None:intergenic 25.0%
! AAATTATTGAGCTATGTAGA+AGG - chr8_2:69959066-69959085 None:intergenic 25.0%
! TGAATTAAGTACTACTGTTA+AGG + chr8_2:69959220-69959239 Msa1265560:intron 25.0%
! GAATTAAGTACTACTGTTAA+GGG + chr8_2:69959221-69959240 Msa1265560:intron 25.0%
! GTAACATGAACAATAGATTT+AGG - chr8_2:69959321-69959340 None:intergenic 25.0%
! ATTGACATGAAAATTGAAGT+AGG - chr8_2:69959346-69959365 None:intergenic 25.0%
! CAATTCAATACAAATTCTCT+TGG + chr8_2:69959672-69959691 Msa1265560:intron 25.0%
! AACTGAAACAAAAATCCAAA+TGG - chr8_2:69959874-69959893 None:intergenic 25.0%
! GAAACTAGAAACCAAAATTT+AGG - chr8_2:69959960-69959979 None:intergenic 25.0%
! TAACTTATTGCTCTTTACTA+AGG - chr8_2:69960265-69960284 None:intergenic 25.0%
! GAGTATTATTATCAGAACAA+AGG + chr8_2:69960309-69960328 Msa1265560:CDS 25.0%
! TTCATGTAATGTAACATTTG+TGG - chr8_2:69960538-69960557 None:intergenic 25.0%
!! ACAAATTTTCGTTTACTACA+AGG + chr8_2:69960652-69960671 Msa1265560:intron 25.0%
!!! TAACCCTTATTGATTTTGAA+AGG + chr8_2:69960779-69960798 Msa1265560:intron 25.0%
! TGTCCTTTCAAAATCAATAA+GGG - chr8_2:69960785-69960804 None:intergenic 25.0%
! ATGTCCTTTCAAAATCAATA+AGG - chr8_2:69960786-69960805 None:intergenic 25.0%
!! AGTTAGGAATCATATGTTTT+TGG + chr8_2:69960835-69960854 Msa1265560:intron 25.0%
!! TGTTTTTCTATGTCATTCAT+TGG - chr8_2:69960957-69960976 None:intergenic 25.0%
!! TCTTTGTTGTTTCTTAGTGA+AGG + chr8_2:69958520-69958539 Msa1265560:five_prime_UTR 30.0%
!! CTTTGTTGTTTCTTAGTGAA+GGG + chr8_2:69958521-69958540 Msa1265560:five_prime_UTR 30.0%
AAAGATCAATCCAACAACAT+GGG - chr8_2:69958597-69958616 None:intergenic 30.0%
ATTGGAGTTGAGTGTAAAAA+TGG + chr8_2:69958675-69958694 Msa1265560:five_prime_UTR 30.0%
! ATTCTGCTACTTCTTATTAC+AGG + chr8_2:69958749-69958768 Msa1265560:five_prime_UTR 30.0%
AATATGTATCTGTGAAGTGT+AGG - chr8_2:69958793-69958812 None:intergenic 30.0%
TTTAATGCTTCTTTAGTCCA+TGG + chr8_2:69958934-69958953 Msa1265560:intron 30.0%
ACATAAACTTAGACACTATG+AGG - chr8_2:69959039-69959058 None:intergenic 30.0%
TACTACTGTTAAGGGATTTA+TGG + chr8_2:69959229-69959248 Msa1265560:intron 30.0%
TAACAATGAAACCAACATGT+TGG - chr8_2:69959298-69959317 None:intergenic 30.0%
!!! TGTTTTAATTGGTTCAACCA+TGG - chr8_2:69959570-69959589 None:intergenic 30.0%
GAACCAATTAAAACATGACT+TGG + chr8_2:69959575-69959594 Msa1265560:intron 30.0%
!!! TCTCCAAGTCATGTTTTAAT+TGG - chr8_2:69959581-69959600 None:intergenic 30.0%
!!! AATGTTTTAAAAATCGGTCC+AGG - chr8_2:69959627-69959646 None:intergenic 30.0%
!! TCCACTTTTGGTTATTAAAG+TGG + chr8_2:69959714-69959733 Msa1265560:intron 30.0%
TCCACTTTAATAACCAAAAG+TGG - chr8_2:69959718-69959737 None:intergenic 30.0%
! AATTGAAACAGGCAACTTTT+AGG - chr8_2:69959794-69959813 None:intergenic 30.0%
CTGTTTCAATTCACCAAAAA+GGG + chr8_2:69959803-69959822 Msa1265560:intron 30.0%
TTTGTTTCAGTTTCTTGCTA+TGG + chr8_2:69959882-69959901 Msa1265560:intron 30.0%
TTTACCTAGTTTGATCTCAA+CGG + chr8_2:69959932-69959951 Msa1265560:intron 30.0%
GTTGAAAACAGTCTATAGAT+AGG - chr8_2:69960048-69960067 None:intergenic 30.0%
AACCATTGCTAATCATGTAA+GGG - chr8_2:69960183-69960202 None:intergenic 30.0%
AAACCATTGCTAATCATGTA+AGG - chr8_2:69960184-69960203 None:intergenic 30.0%
TAAGGTTGCTACAAGATTTA+TGG - chr8_2:69960247-69960266 None:intergenic 30.0%
ATTATTATCAGAACAAAGGG+AGG + chr8_2:69960313-69960332 Msa1265560:CDS 30.0%
! TATTGATTATAGTCCTGTGA+TGG - chr8_2:69960350-69960369 None:intergenic 30.0%
!! ATGTTGTTTCTATCCATGAT+GGG - chr8_2:69960384-69960403 None:intergenic 30.0%
!! TATGTTGTTTCTATCCATGA+TGG - chr8_2:69960385-69960404 None:intergenic 30.0%
! CATTTGCATTGTCTTTTTGA+TGG - chr8_2:69960412-69960431 None:intergenic 30.0%
TTCAGTGATATGAAATCTTC+AGG - chr8_2:69960463-69960482 None:intergenic 30.0%
GTAAAACCATAGTAATTGGT+AGG + chr8_2:69960560-69960579 Msa1265560:intron 30.0%
TATAAGCCTACCAATTACTA+TGG - chr8_2:69960569-69960588 None:intergenic 30.0%
!!! CATATGTTTTTGGAATTTGC+AGG + chr8_2:69960845-69960864 Msa1265560:intron 30.0%
! ACTTTTATTGGATCATCACT+TGG + chr8_2:69961020-69961039 Msa1265560:CDS 30.0%
AAGATCAATCCAACAACATG+GGG - chr8_2:69958596-69958615 None:intergenic 35.0%
GAAAGATCAATCCAACAACA+TGG - chr8_2:69958598-69958617 None:intergenic 35.0%
! TCTTTCCTTTTTGTCTCTCA+AGG + chr8_2:69958612-69958631 Msa1265560:five_prime_UTR 35.0%
ATTCTCCTTGAGAGACAAAA+AGG - chr8_2:69958620-69958639 None:intergenic 35.0%
GTGGAGTTTCCAAACATATA+TGG + chr8_2:69958881-69958900 Msa1265560:intron 35.0%
ACACATGCACCATATATGTT+TGG - chr8_2:69958893-69958912 None:intergenic 35.0%
TGATTCCCCCACATAAATTT+AGG + chr8_2:69959157-69959176 Msa1265560:intron 35.0%
ACTTAATTCATCTGCAACTC+TGG - chr8_2:69959210-69959229 None:intergenic 35.0%
ATAATCACAGACCAACATGT+TGG + chr8_2:69959284-69959303 Msa1265560:intron 35.0%
AGAACTCTCGATTGAACTAT+AGG + chr8_2:69959448-69959467 Msa1265560:intron 35.0%
!! GAACTATAGGTTGATTGAAC+CGG + chr8_2:69959461-69959480 Msa1265560:intron 35.0%
!! CCTTTTTGGTGAATTGAAAC+AGG - chr8_2:69959805-69959824 None:intergenic 35.0%
CCTGTTTCAATTCACCAAAA+AGG + chr8_2:69959802-69959821 Msa1265560:intron 35.0%
TAATGTCAGCTTATCATGAC+TGG + chr8_2:69959847-69959866 Msa1265560:intron 35.0%
! TCTCAACGGTTCCTAAATTT+TGG + chr8_2:69959946-69959965 Msa1265560:intron 35.0%
TCAAGCTACAATGTGAAGAT+GGG - chr8_2:69960015-69960034 None:intergenic 35.0%
TACCCTTACATGATTAGCAA+TGG + chr8_2:69960178-69960197 Msa1265560:intron 35.0%
AAAGAGCAATAAGTTAGCTG+TGG + chr8_2:69960269-69960288 Msa1265560:CDS 35.0%
AAGAGCAATAAGTTAGCTGT+GGG + chr8_2:69960270-69960289 Msa1265560:CDS 35.0%
TATAGTCCTGTGATGGTTAT+TGG - chr8_2:69960343-69960362 None:intergenic 35.0%
AAAGACAATGCAAATGCATG+AGG + chr8_2:69960416-69960435 Msa1265560:CDS 35.0%
TATGAAATCTTCAGGATACC+TGG - chr8_2:69960455-69960474 None:intergenic 35.0%
CATTTGTGGCACAGATTAAA+TGG - chr8_2:69960524-69960543 None:intergenic 35.0%
!!! ATAAAAGTGTTTTCCACAGC+TGG - chr8_2:69961008-69961027 None:intergenic 35.0%
! GCTGTGGAAAACACTTTTAT+TGG + chr8_2:69961008-69961027 Msa1265560:CDS 35.0%
GTAGATTTGACACTAAGCAT+AGG + chr8_2:69961137-69961156 Msa1265560:CDS 35.0%
AACAGTGTGACATTTACTCA+AGG + chr8_2:69961236-69961255 Msa1265560:CDS 35.0%
TGTAACCTTTCAACTCTACA+TGG + chr8_2:69961306-69961325 Msa1265560:three_prime_UTR 35.0%
CATGAACCATCAAAATTCAG+TGG - chr8_2:69961333-69961352 None:intergenic 35.0%
AGGCTGTTGCAAGAAAAAGA+TGG - chr8_2:69958446-69958465 None:intergenic 40.0%
AGATCAATCCAACAACATGG+GGG - chr8_2:69958595-69958614 None:intergenic 40.0%
GGAGTTGAGTGTAAAAATGG+TGG + chr8_2:69958678-69958697 Msa1265560:five_prime_UTR 40.0%
AGTGTAAAAATGGTGGCATG+AGG + chr8_2:69958685-69958704 Msa1265560:five_prime_UTR 40.0%
GTGTAAAAATGGTGGCATGA+GGG + chr8_2:69958686-69958705 Msa1265560:five_prime_UTR 40.0%
TGAGGCCTAAATTTATGTGG+GGG - chr8_2:69959165-69959184 None:intergenic 40.0%
GTGAGGCCTAAATTTATGTG+GGG - chr8_2:69959166-69959185 None:intergenic 40.0%
GGTGAGGCCTAAATTTATGT+GGG - chr8_2:69959167-69959186 None:intergenic 40.0%
AGGTGAGGCCTAAATTTATG+TGG - chr8_2:69959168-69959187 None:intergenic 40.0%
AGAAAATCCAACACAAGGTG+AGG - chr8_2:69959183-69959202 None:intergenic 40.0%
! ACCTTGTGTTGGATTTTCTG+AGG + chr8_2:69959184-69959203 Msa1265560:intron 40.0%
TTATATGATGACAGCAGCAG+TGG - chr8_2:69959268-69959287 None:intergenic 40.0%
! TCTCTATCTCTCTCCACTTT+TGG + chr8_2:69959702-69959721 Msa1265560:intron 40.0%
!! GCAACTTTTAGGAAGGTTTC+TGG - chr8_2:69959783-69959802 None:intergenic 40.0%
!! GAAACAGGCAACTTTTAGGA+AGG - chr8_2:69959790-69959809 None:intergenic 40.0%
! GATACTGTAACTGCCCTTTT+TGG - chr8_2:69959819-69959838 None:intergenic 40.0%
! CTTATCATGACTGGTCCATT+TGG + chr8_2:69959856-69959875 Msa1265560:intron 40.0%
!! TCTTGCTATGGTACTGATTG+TGG + chr8_2:69959894-69959913 Msa1265560:intron 40.0%
CAAGCTACAATGTGAAGATG+GGG - chr8_2:69960014-69960033 None:intergenic 40.0%
CTCAAGCTACAATGTGAAGA+TGG - chr8_2:69960016-69960035 None:intergenic 40.0%
TCTTCACATTGTAGCTTGAG+AGG + chr8_2:69960016-69960035 Msa1265560:intron 40.0%
TAAATTGCAAGGTCCCATCA+TGG + chr8_2:69960368-69960387 Msa1265560:CDS 40.0%
!! TCATCACTTGGCATTGATGA+AGG + chr8_2:69961032-69961051 Msa1265560:CDS 40.0%
GATTTGACACTAAGCATAGG+AGG + chr8_2:69961140-69961159 Msa1265560:CDS 40.0%
! CATGGACCACTGAATTTTGA+TGG + chr8_2:69961324-69961343 Msa1265560:three_prime_UTR 40.0%
TAAAAATGGTGGCATGAGGG+AGG + chr8_2:69958689-69958708 Msa1265560:five_prime_UTR 45.0%
AGCAGAATTGCAGCTTTCTC+TGG - chr8_2:69958737-69958756 None:intergenic 45.0%
ATTTAGGCCTCACCTTGTGT+TGG + chr8_2:69959173-69959192 Msa1265560:intron 45.0%
GCCTCAGAAAATCCAACACA+AGG - chr8_2:69959188-69959207 None:intergenic 45.0%
ATTGGTCGAACTGGCTGATT+CGG - chr8_2:69959382-69959401 None:intergenic 45.0%
TTCGGTTCAATTGGTCGAAC+TGG - chr8_2:69959391-69959410 None:intergenic 45.0%
!!! TGGCTGGTTTTCGGTTCAAT+TGG - chr8_2:69959400-69959419 None:intergenic 45.0%
TGAACCATCTGCTGTTGAAC+CGG - chr8_2:69959483-69959502 None:intergenic 45.0%
TTTGTCATCCGGTTCAACCA+TGG + chr8_2:69959550-69959569 Msa1265560:intron 45.0%
TGGTTCAACCATGGTTGAAC+CGG - chr8_2:69959561-69959580 None:intergenic 45.0%
AATCGGTCCAGGAATCGAAT+CGG - chr8_2:69959616-69959635 None:intergenic 45.0%
AGTGGAGAGAGATAGAGAGA+GGG - chr8_2:69959700-69959719 None:intergenic 45.0%
AAGTGGAGAGAGATAGAGAG+AGG - chr8_2:69959701-69959720 None:intergenic 45.0%
!! ATGGTACTGATTGTGGTCTC+AGG + chr8_2:69959901-69959920 Msa1265560:intron 45.0%
GGAACCGTTGAGATCAAACT+AGG - chr8_2:69959939-69959958 None:intergenic 45.0%
AGCAATAAGTTAGCTGTGGG+TGG + chr8_2:69960273-69960292 Msa1265560:CDS 45.0%
TGAGGACATCTTCAAACACC+AGG + chr8_2:69960434-69960453 Msa1265560:CDS 45.0%
TGATCTTGAAAAGCCAGCTG+TGG + chr8_2:69960992-69961011 Msa1265560:CDS 45.0%
!! ACTTGGCATTGATGAAGGTG+AGG + chr8_2:69961037-69961056 Msa1265560:CDS 45.0%
! GCATTGATGAAGGTGAGGTA+GGG + chr8_2:69961042-69961061 Msa1265560:CDS 45.0%
! AAATGCAGCAGTGTTGGAAC+TGG - chr8_2:69961069-69961088 None:intergenic 45.0%
TCACACTGTTGCTCCTAATG+GGG - chr8_2:69961226-69961245 None:intergenic 45.0%
GTCACACTGTTGCTCCTAAT+GGG - chr8_2:69961227-69961246 None:intergenic 45.0%
TGTCACACTGTTGCTCCTAA+TGG - chr8_2:69961228-69961247 None:intergenic 45.0%
GTGGTCCATGTAGAGTTGAA+AGG - chr8_2:69961314-69961333 None:intergenic 45.0%
!! ATAAAAAAAGTAAAAAAATT+AGG + chr8_2:69960112-69960131 Msa1265560:intron 5.0%
!!! CCAGCGAATTGGCTGGTTTT+CGG - chr8_2:69959409-69959428 None:intergenic 50.0%
AAACCAGCCAATTCGCTGGT+TGG + chr8_2:69959410-69959429 Msa1265560:intron 50.0%
CCAACAACCAACCAGCGAAT+TGG - chr8_2:69959420-69959439 None:intergenic 50.0%
! CCAATTCGCTGGTTGGTTGT+TGG + chr8_2:69959417-69959436 Msa1265560:intron 50.0%
! TGAACCGGTTCAACAGCAGA+TGG + chr8_2:69959476-69959495 Msa1265560:intron 50.0%
TGGAGAGAGATAGAGAGAGG+GGG - chr8_2:69959698-69959717 None:intergenic 50.0%
GTGGAGAGAGATAGAGAGAG+GGG - chr8_2:69959699-69959718 None:intergenic 50.0%
TGTGGGTGGAGTGAATGTGT+GGG + chr8_2:69960287-69960306 Msa1265560:CDS 50.0%
GGCTGACCAATAACCATCAC+AGG + chr8_2:69960334-69960353 Msa1265560:CDS 50.0%
!! GGCATTGATGAAGGTGAGGT+AGG + chr8_2:69961041-69961060 Msa1265560:CDS 50.0%
!! GCTCTGAAATGCAGCAGTGT+TGG - chr8_2:69961075-69961094 None:intergenic 50.0%
GTGACACAACCAACCCCATT+AGG + chr8_2:69961210-69961229 Msa1265560:CDS 50.0%
ACTGTTGCTCCTAATGGGGT+TGG - chr8_2:69961222-69961241 None:intergenic 50.0%
CTCAGGATCCCCCATGTTGT+TGG + chr8_2:69958584-69958603 Msa1265560:five_prime_UTR 55.0%
ATGATGACAGCAGCAGTGGC+AGG - chr8_2:69959264-69959283 None:intergenic 55.0%
CCGAAAACCAGCCAATTCGC+TGG + chr8_2:69959406-69959425 Msa1265560:intron 55.0%
CAACCAACCAGCGAATTGGC+TGG - chr8_2:69959416-69959435 None:intergenic 55.0%
!! TGTCTCGCCGATTCGATTCC+TGG + chr8_2:69959606-69959625 Msa1265560:intron 55.0%
CTGTGGGTGGAGTGAATGTG+TGG + chr8_2:69960286-69960305 Msa1265560:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 69958343 69961412 69958343 ID=Msa1265560;Name=Msa1265560
chr8_2 mRNA 69958343 69961412 69958343 ID=Msa1265560-mRNA-1;Parent=Msa1265560;Name=Msa1265560-mRNA-1;_AED=0.10;_eAED=0.10;_QI=430|1|1|1|0.5|1|3|138|213
chr8_2 exon 69958343 69958770 69958343 ID=Msa1265560-mRNA-1:exon:23142;Parent=Msa1265560-mRNA-1
chr8_2 exon 69960220 69960455 69960220 ID=Msa1265560-mRNA-1:exon:23143;Parent=Msa1265560-mRNA-1
chr8_2 exon 69960867 69961412 69960867 ID=Msa1265560-mRNA-1:exon:23144;Parent=Msa1265560-mRNA-1
chr8_2 five_prime_UTR 69958343 69958770 69958343 ID=Msa1265560-mRNA-1:five_prime_utr;Parent=Msa1265560-mRNA-1
chr8_2 five_prime_UTR 69960220 69960221 69960220 ID=Msa1265560-mRNA-1:five_prime_utr;Parent=Msa1265560-mRNA-1
chr8_2 CDS 69960222 69960455 69960222 ID=Msa1265560-mRNA-1:cds;Parent=Msa1265560-mRNA-1
chr8_2 CDS 69960867 69961274 69960867 ID=Msa1265560-mRNA-1:cds;Parent=Msa1265560-mRNA-1
chr8_2 three_prime_UTR 69961275 69961412 69961275 ID=Msa1265560-mRNA-1:three_prime_utr;Parent=Msa1265560-mRNA-1
Gene Sequence

>Msa1265560

ATGGGAACCAAAATTGAATGTTCCATAAATCTTGTAGCAACCTTAGTAAAGAGCAATAAGTTAGCTGTGGGTGGAGTGAATGTGTGGGAGTATTATTATCAGAACAAAGGGAGGCTGACCAATAACCATCACAGGACTATAATCAATAAATTGCAAGGTCCCATCATGGATAGAAACAACATAGAATCCATCAAAAAGACAATGCAAATGCATGAGGACATCTTCAAACACCAGGTAAAAGAACTTCACAGAGTATACAGTGTGCAAAAGATGCTGATGAATGAGCTAAAAAATAAAATTAGACAACAAAATTTTTGGAATCCAATGAATGACATAGAAAAACAATTTTCAAGAGGTTTTGATCTTGAAAAGCCAGCTGTGGAAAACACTTTTATTGGATCATCACTTGGCATTGATGAAGGTGAGGTAGGGACCAGTTCCAACACTGCTGCATTTCAGAGCTGTAAATTAAGTACTTTTGATGATTTTAATGAAGAATTGGAAGTAGATTTGACACTAAGCATAGGAGGTAGCCAACTTAAGAATTCTAATATGAAACTTCAATTAGGAGAATGCAGTGACACAACCAACCCCATTAGGAGCAACAGTGTGACATTTACTCAAGGTTTACAGCTTAAATAG
Protein sequence

>Msa1265560

MGTKIECSINLVATLVKSNKLAVGGVNVWEYYYQNKGRLTNNHHRTIINKLQGPIMDRNNIESIKKTMQMHEDIFKHQVKELHRVYSVQKMLMNELKNKIRQQNFWNPMNDIEKQFSRGFDLEKPAVENTFIGSSLGIDEGEVGTSSNTAAFQSCKLSTFDDFNEELEVDLTLSIGGSQLKNSNMKLQLGECSDTTNPIRSNSVTFTQGLQLK*