AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1365220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365220 MtrunA17_Chr4g0072101 96.855 159 5 0 55 213 5 163 6.79e-114 322
Msa1365220 MtrunA17_Chr2g0298071 41.860 258 100 9 1 213 1 253 2.26e-46 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365220 AT5G57340.1 40.164 122 48 5 55 176 57 153 1.94e-14 70.1
Msa1365220 AT5G57340.2 40.164 122 48 5 55 176 75 171 2.31e-14 70.1

Find 57 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGTTGCTACAAGATTTA+TGG 0.154976 tig0018212:-31399 None:intergenic
ATTTATGGAACATTCAATTT+TGG 0.200899 tig0018212:-31384 None:intergenic
TATAGTCCTGTGATGGTTAT+TGG 0.284417 tig0018212:-31495 None:intergenic
TCTAATATGAAACTTCAATT+AGG 0.300464 tig0018212:+32334 Msa1365220:CDS
ATTCTGCTACTTCTTATTAC+AGG 0.304234 tig0018212:+29905 Msa1365220:five_prime_UTR
TGTCACACTGTTGCTCCTAA+TGG 0.328969 tig0018212:-32380 None:intergenic
GTCACACTGTTGCTCCTAAT+GGG 0.332519 tig0018212:-32379 None:intergenic
ATTCTCCTTGAGAGACAAAA+AGG 0.342116 tig0018212:-29773 None:intergenic
ACTGTGTAAATTAATAATAT+TGG 0.379818 tig0018212:+29813 Msa1365220:five_prime_UTR
TTCTATGATATATCTTTCTC+AGG 0.403651 tig0018212:+29723 Msa1365220:five_prime_UTR
TAACTTATTGCTCTTTACTA+AGG 0.413153 tig0018212:-31417 None:intergenic
AGCAGAATTGCAGCTTTCTC+TGG 0.413595 tig0018212:-29890 None:intergenic
TCTTTGTTGTTTCTTAGTGA+AGG 0.423743 tig0018212:+29676 Msa1365220:five_prime_UTR
CAAATTTAAAACTATAATCA+TGG 0.423848 tig0018212:-29645 None:intergenic
ATTAATAATTCATGCTTGAC+AGG 0.425827 tig0018212:-29619 None:intergenic
ATATTCATACTGTAATATAT+AGG 0.433605 tig0018212:+29499 Msa1365220:five_prime_UTR
CTTTGTTGTTTCTTAGTGAA+GGG 0.433661 tig0018212:+29677 Msa1365220:five_prime_UTR
TATGTTGTTTCTATCCATGA+TGG 0.434780 tig0018212:-31537 None:intergenic
GTGACACAACCAACCCCATT+AGG 0.451171 tig0018212:+32365 Msa1365220:CDS
ATATTATTAATTTACACAGT+TGG 0.463006 tig0018212:-29810 None:intergenic
AATAAAGTAGAAACTAATCT+AGG 0.470222 tig0018212:-32518 None:intergenic
ATGTTGTTTCTATCCATGAT+GGG 0.484028 tig0018212:-31536 None:intergenic
TCATATTAGAATTCTTAAGT+TGG 0.490514 tig0018212:-32321 None:intergenic
AGTATTATTATCAGAACAAA+GGG 0.491849 tig0018212:+31465 Msa1365220:CDS
TGTAACCTTTCAACTCTACA+TGG 0.503587 tig0018212:+32461 Msa1365220:three_prime_UTR
GCTCTGAAATGCAGCAGTGT+TGG 0.531063 tig0018212:-32227 None:intergenic
CTGTGGGTGGAGTGAATGTG+TGG 0.533528 tig0018212:+31441 Msa1365220:CDS
AAATGCAGCAGTGTTGGAAC+TGG 0.538023 tig0018212:-32221 None:intergenic
ACTGTTGCTCCTAATGGGGT+TGG 0.541229 tig0018212:-32374 None:intergenic
TATTGATTATAGTCCTGTGA+TGG 0.542206 tig0018212:-31502 None:intergenic
TAAATTGCAAGGTCCCATCA+TGG 0.552827 tig0018212:+31523 Msa1365220:CDS
AAATTTAAAACTATAATCAT+GGG 0.553892 tig0018212:-29644 None:intergenic
GGCTGACCAATAACCATCAC+AGG 0.553998 tig0018212:+31489 Msa1365220:CDS
GTGGTCCATGTAGAGTTGAA+AGG 0.563193 tig0018212:-32466 None:intergenic
GGCATTGATGAAGGTGAGGT+AGG 0.564515 tig0018212:+32196 Msa1365220:CDS
TCATCACTTGGCATTGATGA+AGG 0.574340 tig0018212:+32187 Msa1365220:CDS
ACTATAATCAATAAATTGCA+AGG 0.575944 tig0018212:+31512 Msa1365220:CDS
GATTTGACACTAAGCATAGG+AGG 0.578450 tig0018212:+32295 Msa1365220:CDS
AAAGAGCAATAAGTTAGCTG+TGG 0.583848 tig0018212:+31424 Msa1365220:CDS
GCATTGATGAAGGTGAGGTA+GGG 0.591301 tig0018212:+32197 Msa1365220:CDS
TGATCTTGAAAAGCCAGCTG+TGG 0.591394 tig0018212:+32147 Msa1365220:CDS
AGCAATAAGTTAGCTGTGGG+TGG 0.591461 tig0018212:+31428 Msa1365220:CDS
AACAGTGTGACATTTACTCA+AGG 0.600555 tig0018212:+32391 Msa1365220:CDS
AAAGATCAATCCAACAACAT+GGG 0.607629 tig0018212:-29750 None:intergenic
AAGATCAATCCAACAACATG+GGG 0.615061 tig0018212:-29749 None:intergenic
GAGTATTATTATCAGAACAA+AGG 0.615462 tig0018212:+31464 Msa1365220:CDS
GAAAGATCAATCCAACAACA+TGG 0.619463 tig0018212:-29751 None:intergenic
AAGAGCAATAAGTTAGCTGT+GGG 0.620145 tig0018212:+31425 Msa1365220:CDS
CATGAACCATCAAAATTCAG+TGG 0.623614 tig0018212:-32485 None:intergenic
TGTGGGTGGAGTGAATGTGT+GGG 0.624316 tig0018212:+31442 Msa1365220:CDS
GTAGATTTGACACTAAGCAT+AGG 0.634592 tig0018212:+32292 Msa1365220:CDS
TCACACTGTTGCTCCTAATG+GGG 0.647566 tig0018212:-32378 None:intergenic
ATTATTATCAGAACAAAGGG+AGG 0.652018 tig0018212:+31468 Msa1365220:CDS
ACTTGGCATTGATGAAGGTG+AGG 0.653122 tig0018212:+32192 Msa1365220:CDS
AAAGACAATGCAAATGCATG+AGG 0.654368 tig0018212:+31571 Msa1365220:CDS
TGAGGACATCTTCAAACACC+AGG 0.656291 tig0018212:+31589 Msa1365220:CDS
AGATCAATCCAACAACATGG+GGG 0.746907 tig0018212:-29748 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAAAACTATAATCAT+GGG - tig0018212:29647-29666 None:intergenic 10.0%
!!! TAATGGATATAATTATTTTT+TGG + tig0018212:30007-30026 Msa1365220:intron 10.0%
!! AAAAAAATAGAAAAAAAAAC+TGG - tig0018212:30663-30682 None:intergenic 10.0%
!! ATATTCATACTGTAATATAT+AGG + tig0018212:29499-29518 Msa1365220:five_prime_UTR 15.0%
!!! CAAATTTAAAACTATAATCA+TGG - tig0018212:29648-29667 None:intergenic 15.0%
!! ATATTATTAATTTACACAGT+TGG - tig0018212:29813-29832 None:intergenic 15.0%
!! ACTGTGTAAATTAATAATAT+TGG + tig0018212:29813-29832 Msa1365220:five_prime_UTR 15.0%
!! ATAATTATATCCATTACATT+AGG - tig0018212:30003-30022 None:intergenic 15.0%
!!! TGAACAAATGTTTTAAAAAT+CGG - tig0018212:30788-30807 None:intergenic 15.0%
!!! TGGTTTTAATTTTTGTATTT+AGG + tig0018212:31353-31372 Msa1365220:intron 15.0%
!!! TTAATTTTTGTATTTAGGTA+TGG + tig0018212:31358-31377 Msa1365220:intron 15.0%
!!! TAATTTTTGTATTTAGGTAT+GGG + tig0018212:31359-31378 Msa1365220:intron 15.0%
!!! TTAACTTGAATTATGTATTT+GGG - tig0018212:31652-31671 None:intergenic 15.0%
!!! ATTAACTTGAATTATGTATT+TGG - tig0018212:31653-31672 None:intergenic 15.0%
!!! ATTTTCAAGTTTACTAAAAT+TGG + tig0018212:31773-31792 Msa1365220:intron 15.0%
!!! AAATTAGACAACAAAATTTT+TGG + tig0018212:32083-32102 Msa1365220:CDS 15.0%
!!! GTATAACTAATTCAAGTTAA+TGG - tig0018212:29977-29996 None:intergenic 20.0%
!!! ATTATTTTTTGGTGTTTATG+TGG + tig0018212:30018-30037 Msa1365220:intron 20.0%
!!! TTATTTTTTATGTATCACCA+TGG - tig0018212:30110-30129 None:intergenic 20.0%
!!! TACATAGCTCAATAATTTTA+TGG + tig0018212:30224-30243 Msa1365220:intron 20.0%
!!! AGTGAAAAATTTTATGAGAT+GGG - tig0018212:30292-30311 None:intergenic 20.0%
!!! TAGTGAAAAATTTTATGAGA+TGG - tig0018212:30293-30312 None:intergenic 20.0%
!! GTATTTATTTATTTGTCATC+CGG + tig0018212:30694-30713 Msa1365220:intron 20.0%
!!! CTTATTTTCATAAGTTTTTC+AGG + tig0018212:31222-31241 Msa1365220:intron 20.0%
!!! TTTATTTTCATGCTGTTTAT+AGG - tig0018212:31252-31271 None:intergenic 20.0%
!!! ATTTATGGAACATTCAATTT+TGG - tig0018212:31387-31406 None:intergenic 20.0%
!! AGTATTATTATCAGAACAAA+GGG + tig0018212:31465-31484 Msa1365220:CDS 20.0%
!! ACTATAATCAATAAATTGCA+AGG + tig0018212:31512-31531 Msa1365220:CDS 20.0%
!! AAAAGTAAAACCATAGTAAT+TGG + tig0018212:31711-31730 Msa1365220:intron 20.0%
!!! AATTTTAATCTCTACTAGTT+AGG + tig0018212:31974-31993 Msa1365220:intron 20.0%
!!! ATAGAAAAACAATTTTCAAG+AGG + tig0018212:32121-32140 Msa1365220:CDS 20.0%
!!! TGATGATTTTAATGAAGAAT+TGG + tig0018212:32267-32286 Msa1365220:CDS 20.0%
!! TCATATTAGAATTCTTAAGT+TGG - tig0018212:32324-32343 None:intergenic 20.0%
!! TCTAATATGAAACTTCAATT+AGG + tig0018212:32334-32353 Msa1365220:CDS 20.0%
!! AATAAAGTAGAAACTAATCT+AGG - tig0018212:32521-32540 None:intergenic 20.0%
! ATTAATAATTCATGCTTGAC+AGG - tig0018212:29622-29641 None:intergenic 25.0%
! TTCTATGATATATCTTTCTC+AGG + tig0018212:29723-29742 Msa1365220:five_prime_UTR 25.0%
! GTTATACAATCCTAATGTAA+TGG + tig0018212:29990-30009 Msa1365220:intron 25.0%
! AAATTATTGAGCTATGTAGA+AGG - tig0018212:30222-30241 None:intergenic 25.0%
! TGAATTAAGTACTACTGTTA+AGG + tig0018212:30376-30395 Msa1365220:intron 25.0%
! GAATTAAGTACTACTGTTAA+GGG + tig0018212:30377-30396 Msa1365220:intron 25.0%
! GTAACATGAACAATAGATTT+AGG - tig0018212:30477-30496 None:intergenic 25.0%
! ATTGACATGAAAATTGAAGT+AGG - tig0018212:30502-30521 None:intergenic 25.0%
! CAATTCAATACAAATTCTCT+TGG + tig0018212:30827-30846 Msa1365220:intron 25.0%
! AACTGAAACAAAAATCCAAA+TGG - tig0018212:31029-31048 None:intergenic 25.0%
! GAAACTAGAAACCAAAATTT+AGG - tig0018212:31115-31134 None:intergenic 25.0%
! TAACTTATTGCTCTTTACTA+AGG - tig0018212:31420-31439 None:intergenic 25.0%
! GAGTATTATTATCAGAACAA+AGG + tig0018212:31464-31483 Msa1365220:CDS 25.0%
! TTCATGTAATGTAACATTTG+TGG - tig0018212:31693-31712 None:intergenic 25.0%
!! ACAAATTTTCGTTTACTACA+AGG + tig0018212:31807-31826 Msa1365220:intron 25.0%
!!! TAACCCTTATTGATTTTGAA+AGG + tig0018212:31934-31953 Msa1365220:intron 25.0%
! TGTCCTTTCAAAATCAATAA+GGG - tig0018212:31940-31959 None:intergenic 25.0%
! ATGTCCTTTCAAAATCAATA+AGG - tig0018212:31941-31960 None:intergenic 25.0%
!! AGTTAGGAATCATATGTTTT+TGG + tig0018212:31990-32009 Msa1365220:intron 25.0%
!! TGTTTTTCTATGTCATTCAT+TGG - tig0018212:32112-32131 None:intergenic 25.0%
!! TCTTTGTTGTTTCTTAGTGA+AGG + tig0018212:29676-29695 Msa1365220:five_prime_UTR 30.0%
!! CTTTGTTGTTTCTTAGTGAA+GGG + tig0018212:29677-29696 Msa1365220:five_prime_UTR 30.0%
AAAGATCAATCCAACAACAT+GGG - tig0018212:29753-29772 None:intergenic 30.0%
ATTGGAGTTGAGTGTAAAAA+TGG + tig0018212:29831-29850 Msa1365220:five_prime_UTR 30.0%
! ATTCTGCTACTTCTTATTAC+AGG + tig0018212:29905-29924 Msa1365220:five_prime_UTR 30.0%
AATATGTATCTGTGAAGTGT+AGG - tig0018212:29949-29968 None:intergenic 30.0%
TTTAATGCTTCTTTAGTCCA+TGG + tig0018212:30090-30109 Msa1365220:intron 30.0%
GAGTTTACAATTACAAGTCA+AGG - tig0018212:30173-30192 None:intergenic 30.0%
ACATAAACTTAGACACTATG+AGG - tig0018212:30195-30214 None:intergenic 30.0%
TACTACTGTTAAGGGATTTA+TGG + tig0018212:30385-30404 Msa1365220:intron 30.0%
TAACAATGAAACCAACATGT+TGG - tig0018212:30454-30473 None:intergenic 30.0%
!!! TGTTTTAATTGGTTCAACCA+TGG - tig0018212:30725-30744 None:intergenic 30.0%
GAACCAATTAAAACATGACT+TGG + tig0018212:30730-30749 Msa1365220:intron 30.0%
!!! TCTCCAAGTCATGTTTTAAT+TGG - tig0018212:30736-30755 None:intergenic 30.0%
!!! AATGTTTTAAAAATCGGTCC+AGG - tig0018212:30782-30801 None:intergenic 30.0%
!! TCCACTTTTGGTTATTAAAG+TGG + tig0018212:30869-30888 Msa1365220:intron 30.0%
TCCACTTTAATAACCAAAAG+TGG - tig0018212:30873-30892 None:intergenic 30.0%
! AATTGAAACAGGCAACTTTT+AGG - tig0018212:30949-30968 None:intergenic 30.0%
CTGTTTCAATTCACCAAAAA+GGG + tig0018212:30958-30977 Msa1365220:intron 30.0%
TTTGTTTCAGTTTCTTGCTA+TGG + tig0018212:31037-31056 Msa1365220:intron 30.0%
TTTACCTAGTTTGATCTCAA+CGG + tig0018212:31087-31106 Msa1365220:intron 30.0%
GTTGAAAACAGTCTATAGAT+AGG - tig0018212:31203-31222 None:intergenic 30.0%
AACCATTGCTAATCATGTAA+GGG - tig0018212:31338-31357 None:intergenic 30.0%
AAACCATTGCTAATCATGTA+AGG - tig0018212:31339-31358 None:intergenic 30.0%
TAAGGTTGCTACAAGATTTA+TGG - tig0018212:31402-31421 None:intergenic 30.0%
ATTATTATCAGAACAAAGGG+AGG + tig0018212:31468-31487 Msa1365220:CDS 30.0%
! TATTGATTATAGTCCTGTGA+TGG - tig0018212:31505-31524 None:intergenic 30.0%
!! ATGTTGTTTCTATCCATGAT+GGG - tig0018212:31539-31558 None:intergenic 30.0%
!! TATGTTGTTTCTATCCATGA+TGG - tig0018212:31540-31559 None:intergenic 30.0%
! CATTTGCATTGTCTTTTTGA+TGG - tig0018212:31567-31586 None:intergenic 30.0%
TTCAGTGATATGAAATCTTC+AGG - tig0018212:31618-31637 None:intergenic 30.0%
GTAAAACCATAGTAATTGGT+AGG + tig0018212:31715-31734 Msa1365220:intron 30.0%
TATAAGCCTACCAATTACTA+TGG - tig0018212:31724-31743 None:intergenic 30.0%
!!! CATATGTTTTTGGAATTTGC+AGG + tig0018212:32000-32019 Msa1365220:intron 30.0%
! ACTTTTATTGGATCATCACT+TGG + tig0018212:32175-32194 Msa1365220:CDS 30.0%
AAGATCAATCCAACAACATG+GGG - tig0018212:29752-29771 None:intergenic 35.0%
GAAAGATCAATCCAACAACA+TGG - tig0018212:29754-29773 None:intergenic 35.0%
! TCTTTCCTTTTTGTCTCTCA+AGG + tig0018212:29768-29787 Msa1365220:five_prime_UTR 35.0%
ATTCTCCTTGAGAGACAAAA+AGG - tig0018212:29776-29795 None:intergenic 35.0%
GTGGAGTTTCCAAACATATA+TGG + tig0018212:30037-30056 Msa1365220:intron 35.0%
ACACATGCACCATATATGTT+TGG - tig0018212:30049-30068 None:intergenic 35.0%
TGATTCCCCCACATAAATTT+AGG + tig0018212:30313-30332 Msa1365220:intron 35.0%
ACTTAATTCATCTGCAACTC+TGG - tig0018212:30366-30385 None:intergenic 35.0%
ATAATCACAGACCAACATGT+TGG + tig0018212:30440-30459 Msa1365220:intron 35.0%
AGAACTCTCGATTGAACTAT+AGG + tig0018212:30604-30623 Msa1365220:intron 35.0%
!! GAACTATAGGTTGATTGAAC+CGG + tig0018212:30617-30636 Msa1365220:intron 35.0%
!! CCTTTTTGGTGAATTGAAAC+AGG - tig0018212:30960-30979 None:intergenic 35.0%
CCTGTTTCAATTCACCAAAA+AGG + tig0018212:30957-30976 Msa1365220:intron 35.0%
TAATGTCAGCTTATCATGAC+TGG + tig0018212:31002-31021 Msa1365220:intron 35.0%
! TCTCAACGGTTCCTAAATTT+TGG + tig0018212:31101-31120 Msa1365220:intron 35.0%
TCAAGCTACAATGTGAAGAT+GGG - tig0018212:31170-31189 None:intergenic 35.0%
TACCCTTACATGATTAGCAA+TGG + tig0018212:31333-31352 Msa1365220:intron 35.0%
AAAGAGCAATAAGTTAGCTG+TGG + tig0018212:31424-31443 Msa1365220:CDS 35.0%
AAGAGCAATAAGTTAGCTGT+GGG + tig0018212:31425-31444 Msa1365220:CDS 35.0%
TATAGTCCTGTGATGGTTAT+TGG - tig0018212:31498-31517 None:intergenic 35.0%
AAAGACAATGCAAATGCATG+AGG + tig0018212:31571-31590 Msa1365220:CDS 35.0%
TATGAAATCTTCAGGATACC+TGG - tig0018212:31610-31629 None:intergenic 35.0%
CATTTGTGGCACAGATTAAA+TGG - tig0018212:31679-31698 None:intergenic 35.0%
!!! ATAAAAGTGTTTTCCACAGC+TGG - tig0018212:32163-32182 None:intergenic 35.0%
! GCTGTGGAAAACACTTTTAT+TGG + tig0018212:32163-32182 Msa1365220:CDS 35.0%
GTAGATTTGACACTAAGCAT+AGG + tig0018212:32292-32311 Msa1365220:CDS 35.0%
AACAGTGTGACATTTACTCA+AGG + tig0018212:32391-32410 Msa1365220:CDS 35.0%
TGTAACCTTTCAACTCTACA+TGG + tig0018212:32461-32480 Msa1365220:three_prime_UTR 35.0%
CATGAACCATCAAAATTCAG+TGG - tig0018212:32488-32507 None:intergenic 35.0%
AGGCTGTTGCAAGAAAAAGA+TGG - tig0018212:29602-29621 None:intergenic 40.0%
AGATCAATCCAACAACATGG+GGG - tig0018212:29751-29770 None:intergenic 40.0%
GGAGTTGAGTGTAAAAATGG+TGG + tig0018212:29834-29853 Msa1365220:five_prime_UTR 40.0%
AGTGTAAAAATGGTGGCATG+AGG + tig0018212:29841-29860 Msa1365220:five_prime_UTR 40.0%
GTGTAAAAATGGTGGCATGA+GGG + tig0018212:29842-29861 Msa1365220:five_prime_UTR 40.0%
TGAGGCCTAAATTTATGTGG+GGG - tig0018212:30321-30340 None:intergenic 40.0%
GTGAGGCCTAAATTTATGTG+GGG - tig0018212:30322-30341 None:intergenic 40.0%
GGTGAGGCCTAAATTTATGT+GGG - tig0018212:30323-30342 None:intergenic 40.0%
AGGTGAGGCCTAAATTTATG+TGG - tig0018212:30324-30343 None:intergenic 40.0%
AGAAAATCCAACACAAGGTG+AGG - tig0018212:30339-30358 None:intergenic 40.0%
! ACCTTGTGTTGGATTTTCTG+AGG + tig0018212:30340-30359 Msa1365220:intron 40.0%
TTATATGATGACAGCAGCAG+TGG - tig0018212:30424-30443 None:intergenic 40.0%
! TCTCTATCTCTCTCCACTTT+TGG + tig0018212:30857-30876 Msa1365220:intron 40.0%
!! GCAACTTTTAGGAAGGTTTC+TGG - tig0018212:30938-30957 None:intergenic 40.0%
!! GAAACAGGCAACTTTTAGGA+AGG - tig0018212:30945-30964 None:intergenic 40.0%
! GATACTGTAACTGCCCTTTT+TGG - tig0018212:30974-30993 None:intergenic 40.0%
! CTTATCATGACTGGTCCATT+TGG + tig0018212:31011-31030 Msa1365220:intron 40.0%
!! TCTTGCTATGGTACTGATTG+TGG + tig0018212:31049-31068 Msa1365220:intron 40.0%
CAAGCTACAATGTGAAGATG+GGG - tig0018212:31169-31188 None:intergenic 40.0%
CTCAAGCTACAATGTGAAGA+TGG - tig0018212:31171-31190 None:intergenic 40.0%
TCTTCACATTGTAGCTTGAG+AGG + tig0018212:31171-31190 Msa1365220:intron 40.0%
TAAATTGCAAGGTCCCATCA+TGG + tig0018212:31523-31542 Msa1365220:CDS 40.0%
!! TCATCACTTGGCATTGATGA+AGG + tig0018212:32187-32206 Msa1365220:CDS 40.0%
GATTTGACACTAAGCATAGG+AGG + tig0018212:32295-32314 Msa1365220:CDS 40.0%
! CATGGACCACTGAATTTTGA+TGG + tig0018212:32479-32498 Msa1365220:three_prime_UTR 40.0%
TAAAAATGGTGGCATGAGGG+AGG + tig0018212:29845-29864 Msa1365220:five_prime_UTR 45.0%
AGCAGAATTGCAGCTTTCTC+TGG - tig0018212:29893-29912 None:intergenic 45.0%
ATTTAGGCCTCACCTTGTGT+TGG + tig0018212:30329-30348 Msa1365220:intron 45.0%
GCCTCAGAAAATCCAACACA+AGG - tig0018212:30344-30363 None:intergenic 45.0%
ATTGGTCGAACTGGCTGATT+CGG - tig0018212:30538-30557 None:intergenic 45.0%
TTCGGTTCAATTGGTCGAAC+TGG - tig0018212:30547-30566 None:intergenic 45.0%
!!! TGGCTGGTTTTCGGTTCAAT+TGG - tig0018212:30556-30575 None:intergenic 45.0%
TGAACCATCTGCAGTTGAAC+CGG - tig0018212:30639-30658 None:intergenic 45.0%
TTTGTCATCCGGTTCAACCA+TGG + tig0018212:30705-30724 Msa1365220:intron 45.0%
TGGTTCAACCATGGTTGAAC+CGG - tig0018212:30716-30735 None:intergenic 45.0%
AATCGGTCCAGGAATCGAAT+CGG - tig0018212:30771-30790 None:intergenic 45.0%
AGTGGAGAGAGATAGAGAGA+GGG - tig0018212:30855-30874 None:intergenic 45.0%
AAGTGGAGAGAGATAGAGAG+AGG - tig0018212:30856-30875 None:intergenic 45.0%
!! ATGGTACTGATTGTGGTCTC+AGG + tig0018212:31056-31075 Msa1365220:intron 45.0%
GGAACCGTTGAGATCAAACT+AGG - tig0018212:31094-31113 None:intergenic 45.0%
AGCAATAAGTTAGCTGTGGG+TGG + tig0018212:31428-31447 Msa1365220:CDS 45.0%
TGAGGACATCTTCAAACACC+AGG + tig0018212:31589-31608 Msa1365220:CDS 45.0%
TGATCTTGAAAAGCCAGCTG+TGG + tig0018212:32147-32166 Msa1365220:CDS 45.0%
!! ACTTGGCATTGATGAAGGTG+AGG + tig0018212:32192-32211 Msa1365220:CDS 45.0%
! GCATTGATGAAGGTGAGGTA+GGG + tig0018212:32197-32216 Msa1365220:CDS 45.0%
! AAATGCAGCAGTGTTGGAAC+TGG - tig0018212:32224-32243 None:intergenic 45.0%
TCACACTGTTGCTCCTAATG+GGG - tig0018212:32381-32400 None:intergenic 45.0%
GTCACACTGTTGCTCCTAAT+GGG - tig0018212:32382-32401 None:intergenic 45.0%
TGTCACACTGTTGCTCCTAA+TGG - tig0018212:32383-32402 None:intergenic 45.0%
GTGGTCCATGTAGAGTTGAA+AGG - tig0018212:32469-32488 None:intergenic 45.0%
!! ATAAAAAAAGTAAAAAAATT+AGG + tig0018212:31267-31286 Msa1365220:intron 5.0%
!!! CCAGCGAATTGGCTGGTTTT+CGG - tig0018212:30565-30584 None:intergenic 50.0%
AAACCAGCCAATTCGCTGGT+TGG + tig0018212:30566-30585 Msa1365220:intron 50.0%
CCAACAACCAACCAGCGAAT+TGG - tig0018212:30576-30595 None:intergenic 50.0%
! CCAATTCGCTGGTTGGTTGT+TGG + tig0018212:30573-30592 Msa1365220:intron 50.0%
! TGAACCGGTTCAACTGCAGA+TGG + tig0018212:30632-30651 Msa1365220:intron 50.0%
TGGAGAGAGATAGAGAGAGG+GGG - tig0018212:30853-30872 None:intergenic 50.0%
GTGGAGAGAGATAGAGAGAG+GGG - tig0018212:30854-30873 None:intergenic 50.0%
TGTGGGTGGAGTGAATGTGT+GGG + tig0018212:31442-31461 Msa1365220:CDS 50.0%
GGCTGACCAATAACCATCAC+AGG + tig0018212:31489-31508 Msa1365220:CDS 50.0%
!! GGCATTGATGAAGGTGAGGT+AGG + tig0018212:32196-32215 Msa1365220:CDS 50.0%
!! GCTCTGAAATGCAGCAGTGT+TGG - tig0018212:32230-32249 None:intergenic 50.0%
GTGACACAACCAACCCCATT+AGG + tig0018212:32365-32384 Msa1365220:CDS 50.0%
ACTGTTGCTCCTAATGGGGT+TGG - tig0018212:32377-32396 None:intergenic 50.0%
CTCAGGATCCCCCATGTTGT+TGG + tig0018212:29740-29759 Msa1365220:five_prime_UTR 55.0%
ATGATGACAGCAGCAGTGGC+AGG - tig0018212:30420-30439 None:intergenic 55.0%
CCGAAAACCAGCCAATTCGC+TGG + tig0018212:30562-30581 Msa1365220:intron 55.0%
CAACCAACCAGCGAATTGGC+TGG - tig0018212:30572-30591 None:intergenic 55.0%
!! TGTCTCGCCGATTCGATTCC+TGG + tig0018212:30761-30780 Msa1365220:intron 55.0%
CTGTGGGTGGAGTGAATGTG+TGG + tig0018212:31441-31460 Msa1365220:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0018212 gene 29499 32567 29499 ID=Msa1365220;Name=Msa1365220
tig0018212 mRNA 29499 32567 29499 ID=Msa1365220-mRNA-1;Parent=Msa1365220;Name=Msa1365220-mRNA-1;_AED=0.10;_eAED=0.10;_QI=430|1|1|1|0.5|1|3|138|213
tig0018212 exon 29499 29926 29499 ID=Msa1365220-mRNA-1:exon:14422;Parent=Msa1365220-mRNA-1
tig0018212 exon 31375 31610 31375 ID=Msa1365220-mRNA-1:exon:14423;Parent=Msa1365220-mRNA-1
tig0018212 exon 32022 32567 32022 ID=Msa1365220-mRNA-1:exon:14424;Parent=Msa1365220-mRNA-1
tig0018212 five_prime_UTR 29499 29926 29499 ID=Msa1365220-mRNA-1:five_prime_utr;Parent=Msa1365220-mRNA-1
tig0018212 five_prime_UTR 31375 31376 31375 ID=Msa1365220-mRNA-1:five_prime_utr;Parent=Msa1365220-mRNA-1
tig0018212 CDS 31377 31610 31377 ID=Msa1365220-mRNA-1:cds;Parent=Msa1365220-mRNA-1
tig0018212 CDS 32022 32429 32022 ID=Msa1365220-mRNA-1:cds;Parent=Msa1365220-mRNA-1
tig0018212 three_prime_UTR 32430 32567 32430 ID=Msa1365220-mRNA-1:three_prime_utr;Parent=Msa1365220-mRNA-1
Gene Sequence

>Msa1365220

ATGGGAACCAAAATTGAATGTTCCATAAATCTTGTAGCAACCTTAGTAAAGAGCAATAAGTTAGCTGTGGGTGGAGTGAATGTGTGGGAGTATTATTATCAGAACAAAGGGAGGCTGACCAATAACCATCACAGGACTATAATCAATAAATTGCAAGGTCCCATCATGGATAGAAACAACATAGAATCCATCAAAAAGACAATGCAAATGCATGAGGACATCTTCAAACACCAGGTAAAAGAACTTCACAGAGTATACAGTGTGCAAAAGATGCTGATGAATGAGCTAAAAAATAAAATTAGACAACAAAATTTTTGGAATCCAATGAATGACATAGAAAAACAATTTTCAAGAGGTTTTGATCTTGAAAAGCCAGCTGTGGAAAACACTTTTATTGGATCATCACTTGGCATTGATGAAGGTGAGGTAGGGACCAGTTCCAACACTGCTGCATTTCAGAGCTGTAAATTAAGTACTTTTGATGATTTTAATGAAGAATTGGAAGTAGATTTGACACTAAGCATAGGAGGTAGCCAACTTAAGAATTCTAATATGAAACTTCAATTAGGAGAATGCAGTGACACAACCAACCCCATTAGGAGCAACAGTGTGACATTTACTCAAGGTTTACAGCTTAAATAG
Protein sequence

>Msa1365220

MGTKIECSINLVATLVKSNKLAVGGVNVWEYYYQNKGRLTNNHHRTIINKLQGPIMDRNNIESIKKTMQMHEDIFKHQVKELHRVYSVQKMLMNELKNKIRQQNFWNPMNDIEKQFSRGFDLEKPAVENTFIGSSLGIDEGEVGTSSNTAAFQSCKLSTFDDFNEELEVDLTLSIGGSQLKNSNMKLQLGECSDTTNPIRSNSVTFTQGLQLK*