AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1223440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1223440 MtrunA17_Chr4g0074981 94.286 70 3 1 73 141 1 70 6.67e-42 134
Msa1223440 MtrunA17_Chr2g0288541 48.718 78 23 3 73 134 1 77 7.78e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1223440 AT1G11740.1 44.286 70 36 2 68 134 568 637 6.63e-11 59.3
Msa1223440 AT1G62045.1 47.619 63 30 2 73 134 1 61 6.67e-11 55.1

Find 68 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATATGTTCAAACTTTA+AGG 0.036614 8_1:+87907734 None:intergenic
CTTAAAGTTTGAACATATTT+TGG 0.134469 8_1:-87907733 None:intergenic
CAGCTTTAATTCATCAAAAT+TGG 0.153527 8_1:+87907930 None:intergenic
CTCCCTATATAGTTATAATT+TGG 0.187890 8_1:-87908588 Msa1223440:CDS
CAACCAAACAACTGGGTTAA+TGG 0.209500 8_1:-87907996 Msa1223440:exon
GGTTCAATATTAGAGTATTT+TGG 0.243915 8_1:-87908309 Msa1223440:CDS
CCGCACATAGTTTGCTTAAC+AGG 0.254549 8_1:-87908737 Msa1223440:five_prime_UTR
CAATGGGGCGCTGGTGGTTT+TGG 0.260610 8_1:-87908156 Msa1223440:CDS
ATATTTATCAAAGAAATTAA+TGG 0.274439 8_1:-87908330 Msa1223440:CDS
GTCAAGTCCTATAATGAAAA+AGG 0.284159 8_1:-87908685 Msa1223440:five_prime_UTR
CTGATGCCAACAATGTTAAT+TGG 0.302534 8_1:-87908936 Msa1223440:five_prime_UTR
GAACCAAATTATAACTATAT+AGG 0.317411 8_1:+87908585 None:intergenic
CCTCCGTTGCCATGTTTCCT+AGG 0.323008 8_1:+87908196 None:intergenic
ATACGATAATTAATATATAT+TGG 0.326536 8_1:-87907778 Msa1223440:three_prime_UTR
GCTTGATGGAGGAGTTAAAA+TGG 0.327248 8_1:+87908548 None:intergenic
TTAAAGCTGCTTTATTTAGT+TGG 0.347159 8_1:-87907916 Msa1223440:three_prime_UTR
ACAGTTTGAGGCAGTTGCTT+TGG 0.357175 8_1:+87908056 None:intergenic
TGTCATCCAATTAACATTGT+TGG 0.357308 8_1:+87908930 None:intergenic
CTCCCATTAACCCAGTTGTT+TGG 0.359208 8_1:+87907993 None:intergenic
AACCAAATTATAACTATATA+GGG 0.367674 8_1:+87908586 None:intergenic
AACCAAACAACTGGGTTAAT+GGG 0.369185 8_1:-87907995 Msa1223440:exon
AGTACCTTCAACGATTGAAT+TGG 0.380721 8_1:+87908501 None:intergenic
ACCAAGTTGGGCAGATCAAT+GGG 0.383800 8_1:-87908172 Msa1223440:CDS
AAAGACACCAACCAAACAAC+TGG 0.393330 8_1:-87908004 Msa1223440:CDS
TTGGTTATAGTTTAGCCAAT+TGG 0.413285 8_1:+87908868 None:intergenic
CATTAACCCAGTTGTTTGGT+TGG 0.420387 8_1:+87907997 None:intergenic
TTGTGTGACACTTTCATAGA+AGG 0.425409 8_1:+87908896 None:intergenic
GGCAACGGAGGCACCAAGTT+GGG 0.426929 8_1:-87908184 Msa1223440:CDS
CTCCGTTGCCATGTTTCCTA+GGG 0.430308 8_1:+87908197 None:intergenic
TGCCACCACTAAGATATCTT+AGG 0.431120 8_1:+87908845 None:intergenic
AATTGGATGACAAATACCTT+TGG 0.433271 8_1:-87908919 Msa1223440:five_prime_UTR
AGAAGAATTGAGAAGCTTGA+TGG 0.445967 8_1:+87908534 None:intergenic
CACCAAGTTGGGCAGATCAA+TGG 0.454043 8_1:-87908173 Msa1223440:CDS
TTGGTGTCTTTCTCTTGCAA+AGG 0.463805 8_1:+87908016 None:intergenic
CCCCATTGATCTGCCCAACT+TGG 0.464010 8_1:+87908171 None:intergenic
AAATCCACTATTATATGATA+AGG 0.468902 8_1:-87908794 Msa1223440:five_prime_UTR
TAACCACTTTACACAGTTTG+AGG 0.477436 8_1:+87908044 None:intergenic
TGGGCAGATCAATGGGGCGC+TGG 0.484143 8_1:-87908165 Msa1223440:CDS
TATAGTTTAGCCAATTGGTA+TGG 0.493357 8_1:+87908873 None:intergenic
TCAACCTTATCATATAATAG+TGG 0.493986 8_1:+87908790 None:intergenic
TTGGCTTTGCATAAAGCACT+TGG 0.494499 8_1:+87908075 None:intergenic
AACTCCAATTCAATCGTTGA+AGG 0.498435 8_1:-87908505 Msa1223440:intron
ATGGAAATACTCAAAGCATA+AGG 0.503385 8_1:-87908714 Msa1223440:five_prime_UTR
GCAGATCAATGGGGCGCTGG+TGG 0.516112 8_1:-87908162 Msa1223440:CDS
TGTCACACAACCATACCAAT+TGG 0.516367 8_1:-87908883 Msa1223440:five_prime_UTR
TTATTATGGTCTAGGCCCCT+AGG 0.522084 8_1:-87908213 Msa1223440:intron
AGAATTGAGAAGCTTGATGG+AGG 0.529433 8_1:+87908537 None:intergenic
ACCAACCTAAGATATCTTAG+TGG 0.550884 8_1:-87908850 Msa1223440:five_prime_UTR
AAAAGCAATGTAACATTGAG+AGG 0.552251 8_1:-87908636 Msa1223440:five_prime_UTR
CTGCCTCAAACTGTGTAAAG+TGG 0.553790 8_1:-87908047 Msa1223440:CDS
CGATAATTAATATATATTGG+AGG 0.556000 8_1:-87907775 Msa1223440:three_prime_UTR
GTCTAGGCCCCTAGGAAACA+TGG 0.561053 8_1:-87908205 Msa1223440:intron
TGGCAACGGAGGCACCAAGT+TGG 0.565641 8_1:-87908185 Msa1223440:CDS
CATTGAGAGGCTATCTAACA+TGG 0.570352 8_1:-87908623 Msa1223440:exon
ACCACTAAGATATCTTAGGT+TGG 0.581604 8_1:+87908849 None:intergenic
TGATCTTATCAGCTCTAGGA+AGG 0.592270 8_1:+87908281 None:intergenic
ATCTTGATCTTATCAGCTCT+AGG 0.611567 8_1:+87908277 None:intergenic
CCTAGGAAACATGGCAACGG+AGG 0.612608 8_1:-87908196 Msa1223440:CDS
ACATAGTTTGCTTAACAGGA+TGG 0.613222 8_1:-87908733 Msa1223440:five_prime_UTR
GCCCCTAGGAAACATGGCAA+CGG 0.626735 8_1:-87908199 Msa1223440:CDS
TAGAGCTGATAAGATCAAGA+TGG 0.627836 8_1:-87908275 Msa1223440:intron
AACCTAAGATATCTTAGTGG+TGG 0.635605 8_1:-87908847 Msa1223440:five_prime_UTR
TCCGTTGCCATGTTTCCTAG+GGG 0.642931 8_1:+87908198 None:intergenic
ACACTTTCATAGAAGGCCAA+AGG 0.644696 8_1:+87908903 None:intergenic
CCTGTTAAGCAAACTATGTG+CGG 0.646183 8_1:+87908737 None:intergenic
CAAACAACTGGGTTAATGGG+AGG 0.671503 8_1:-87907992 Msa1223440:exon
AAGACACCAACCAAACAACT+GGG 0.684338 8_1:-87908003 Msa1223440:CDS
CCAAGTTGGGCAGATCAATG+GGG 0.697216 8_1:-87908171 Msa1223440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTATCAAAGAAATTAA+TGG - chr8_1:87908347-87908366 Msa1223440:intron 10.0%
!!! TTATAATTAATTTTAGTTGT+AGG - chr8_1:87908824-87908843 Msa1223440:five_prime_UTR 10.0%
!! ATACGATAATTAATATATAT+TGG - chr8_1:87908899-87908918 Msa1223440:five_prime_UTR 10.0%
!! ATATTTATCAAAGAAATTAA+TGG - chr8_1:87908347-87908366 Msa1223440:intron 10.0%
!!! TTATAATTAATTTTAGTTGT+AGG - chr8_1:87908824-87908843 Msa1223440:five_prime_UTR 10.0%
!! ATACGATAATTAATATATAT+TGG - chr8_1:87908899-87908918 Msa1223440:five_prime_UTR 10.0%
!! AACCAAATTATAACTATATA+GGG + chr8_1:87908094-87908113 None:intergenic 15.0%
!! AAATATTACAACATATCAAT+TGG + chr8_1:87908288-87908307 None:intergenic 15.0%
!! AACCAAATTATAACTATATA+GGG + chr8_1:87908094-87908113 None:intergenic 15.0%
!! AAATATTACAACATATCAAT+TGG + chr8_1:87908288-87908307 None:intergenic 15.0%
!! AAATCCACTATTATATGATA+AGG - chr8_1:87907883-87907902 Msa1223440:three_prime_UTR 20.0%
!!! TCTTTTTCCTTTTTCATTAT+AGG + chr8_1:87908002-87908021 None:intergenic 20.0%
!! GAACCAAATTATAACTATAT+AGG + chr8_1:87908095-87908114 None:intergenic 20.0%
!!! TAGTTTTTTTATTATGGTCT+AGG - chr8_1:87908456-87908475 Msa1223440:intron 20.0%
!! CGATAATTAATATATATTGG+AGG - chr8_1:87908902-87908921 Msa1223440:five_prime_UTR 20.0%
!! CAAAATATGTTCAAACTTTA+AGG + chr8_1:87908946-87908965 None:intergenic 20.0%
!! AAATCCACTATTATATGATA+AGG - chr8_1:87907883-87907902 Msa1223440:three_prime_UTR 20.0%
!!! TCTTTTTCCTTTTTCATTAT+AGG + chr8_1:87908002-87908021 None:intergenic 20.0%
!! GAACCAAATTATAACTATAT+AGG + chr8_1:87908095-87908114 None:intergenic 20.0%
!!! TAGTTTTTTTATTATGGTCT+AGG - chr8_1:87908456-87908475 Msa1223440:intron 20.0%
!! CGATAATTAATATATATTGG+AGG - chr8_1:87908902-87908921 Msa1223440:five_prime_UTR 20.0%
!! CAAAATATGTTCAAACTTTA+AGG + chr8_1:87908946-87908965 None:intergenic 20.0%
! TCAACCTTATCATATAATAG+TGG + chr8_1:87907890-87907909 None:intergenic 25.0%
! CTCCCTATATAGTTATAATT+TGG - chr8_1:87908089-87908108 Msa1223440:CDS 25.0%
! TATAGATTGAAATGCTTAGA+TGG + chr8_1:87908212-87908231 None:intergenic 25.0%
!!! GGTTCAATATTAGAGTATTT+TGG - chr8_1:87908368-87908387 Msa1223440:intron 25.0%
!! CAGCTTTAATTCATCAAAAT+TGG + chr8_1:87908750-87908769 None:intergenic 25.0%
!!! TTAAAGCTGCTTTATTTAGT+TGG - chr8_1:87908761-87908780 Msa1223440:five_prime_UTR 25.0%
! TCAACCTTATCATATAATAG+TGG + chr8_1:87907890-87907909 None:intergenic 25.0%
! CTCCCTATATAGTTATAATT+TGG - chr8_1:87908089-87908108 Msa1223440:CDS 25.0%
! TATAGATTGAAATGCTTAGA+TGG + chr8_1:87908212-87908231 None:intergenic 25.0%
!!! GGTTCAATATTAGAGTATTT+TGG - chr8_1:87908368-87908387 Msa1223440:intron 25.0%
!! CAGCTTTAATTCATCAAAAT+TGG + chr8_1:87908750-87908769 None:intergenic 25.0%
!!! TTAAAGCTGCTTTATTTAGT+TGG - chr8_1:87908761-87908780 Msa1223440:five_prime_UTR 25.0%
TGTCATCCAATTAACATTGT+TGG + chr8_1:87907750-87907769 None:intergenic 30.0%
AATTGGATGACAAATACCTT+TGG - chr8_1:87907758-87907777 Msa1223440:three_prime_UTR 30.0%
TATAGTTTAGCCAATTGGTA+TGG + chr8_1:87907807-87907826 None:intergenic 30.0%
TTGGTTATAGTTTAGCCAAT+TGG + chr8_1:87907812-87907831 None:intergenic 30.0%
ATGGAAATACTCAAAGCATA+AGG - chr8_1:87907963-87907982 Msa1223440:three_prime_UTR 30.0%
GTCAAGTCCTATAATGAAAA+AGG - chr8_1:87907992-87908011 Msa1223440:exon 30.0%
AAAAGCAATGTAACATTGAG+AGG - chr8_1:87908041-87908060 Msa1223440:CDS 30.0%
CAACTTATACGATGTTTCAT+TGG - chr8_1:87908255-87908274 Msa1223440:intron 30.0%
TGTCATCCAATTAACATTGT+TGG + chr8_1:87907750-87907769 None:intergenic 30.0%
AATTGGATGACAAATACCTT+TGG - chr8_1:87907758-87907777 Msa1223440:three_prime_UTR 30.0%
TATAGTTTAGCCAATTGGTA+TGG + chr8_1:87907807-87907826 None:intergenic 30.0%
TTGGTTATAGTTTAGCCAAT+TGG + chr8_1:87907812-87907831 None:intergenic 30.0%
ATGGAAATACTCAAAGCATA+AGG - chr8_1:87907963-87907982 Msa1223440:three_prime_UTR 30.0%
GTCAAGTCCTATAATGAAAA+AGG - chr8_1:87907992-87908011 Msa1223440:exon 30.0%
AAAAGCAATGTAACATTGAG+AGG - chr8_1:87908041-87908060 Msa1223440:CDS 30.0%
CAACTTATACGATGTTTCAT+TGG - chr8_1:87908255-87908274 Msa1223440:intron 30.0%
CTGATGCCAACAATGTTAAT+TGG - chr8_1:87907741-87907760 Msa1223440:three_prime_UTR 35.0%
TTGTGTGACACTTTCATAGA+AGG + chr8_1:87907784-87907803 None:intergenic 35.0%
ACCAACCTAAGATATCTTAG+TGG - chr8_1:87907827-87907846 Msa1223440:three_prime_UTR 35.0%
ACCACTAAGATATCTTAGGT+TGG + chr8_1:87907831-87907850 None:intergenic 35.0%
AACCTAAGATATCTTAGTGG+TGG - chr8_1:87907830-87907849 Msa1223440:three_prime_UTR 35.0%
!! ACATAGTTTGCTTAACAGGA+TGG - chr8_1:87907944-87907963 Msa1223440:three_prime_UTR 35.0%
AGAAGAATTGAGAAGCTTGA+TGG + chr8_1:87908146-87908165 None:intergenic 35.0%
AACTCCAATTCAATCGTTGA+AGG - chr8_1:87908172-87908191 Msa1223440:CDS 35.0%
AGTACCTTCAACGATTGAAT+TGG + chr8_1:87908179-87908198 None:intergenic 35.0%
ATCTTGATCTTATCAGCTCT+AGG + chr8_1:87908403-87908422 None:intergenic 35.0%
TAGAGCTGATAAGATCAAGA+TGG - chr8_1:87908402-87908421 Msa1223440:intron 35.0%
TAACCACTTTACACAGTTTG+AGG + chr8_1:87908636-87908655 None:intergenic 35.0%
AACCAAACAACTGGGTTAAT+GGG - chr8_1:87908682-87908701 Msa1223440:five_prime_UTR 35.0%
CTGATGCCAACAATGTTAAT+TGG - chr8_1:87907741-87907760 Msa1223440:three_prime_UTR 35.0%
TTGTGTGACACTTTCATAGA+AGG + chr8_1:87907784-87907803 None:intergenic 35.0%
ACCAACCTAAGATATCTTAG+TGG - chr8_1:87907827-87907846 Msa1223440:three_prime_UTR 35.0%
ACCACTAAGATATCTTAGGT+TGG + chr8_1:87907831-87907850 None:intergenic 35.0%
AACCTAAGATATCTTAGTGG+TGG - chr8_1:87907830-87907849 Msa1223440:three_prime_UTR 35.0%
!! ACATAGTTTGCTTAACAGGA+TGG - chr8_1:87907944-87907963 Msa1223440:three_prime_UTR 35.0%
AGAAGAATTGAGAAGCTTGA+TGG + chr8_1:87908146-87908165 None:intergenic 35.0%
AACTCCAATTCAATCGTTGA+AGG - chr8_1:87908172-87908191 Msa1223440:CDS 35.0%
AGTACCTTCAACGATTGAAT+TGG + chr8_1:87908179-87908198 None:intergenic 35.0%
ATCTTGATCTTATCAGCTCT+AGG + chr8_1:87908403-87908422 None:intergenic 35.0%
TAGAGCTGATAAGATCAAGA+TGG - chr8_1:87908402-87908421 Msa1223440:intron 35.0%
TAACCACTTTACACAGTTTG+AGG + chr8_1:87908636-87908655 None:intergenic 35.0%
AACCAAACAACTGGGTTAAT+GGG - chr8_1:87908682-87908701 Msa1223440:five_prime_UTR 35.0%
ACACTTTCATAGAAGGCCAA+AGG + chr8_1:87907777-87907796 None:intergenic 40.0%
TGTCACACAACCATACCAAT+TGG - chr8_1:87907794-87907813 Msa1223440:three_prime_UTR 40.0%
TGCCACCACTAAGATATCTT+AGG + chr8_1:87907835-87907854 None:intergenic 40.0%
CCTGTTAAGCAAACTATGTG+CGG + chr8_1:87907943-87907962 None:intergenic 40.0%
CATTGAGAGGCTATCTAACA+TGG - chr8_1:87908054-87908073 Msa1223440:CDS 40.0%
GCTTGATGGAGGAGTTAAAA+TGG + chr8_1:87908132-87908151 None:intergenic 40.0%
AGAATTGAGAAGCTTGATGG+AGG + chr8_1:87908143-87908162 None:intergenic 40.0%
!! TGATCTTATCAGCTCTAGGA+AGG + chr8_1:87908399-87908418 None:intergenic 40.0%
!! TTGGCTTTGCATAAAGCACT+TGG + chr8_1:87908605-87908624 None:intergenic 40.0%
! TTGGTGTCTTTCTCTTGCAA+AGG + chr8_1:87908664-87908683 None:intergenic 40.0%
AAAGACACCAACCAAACAAC+TGG - chr8_1:87908673-87908692 Msa1223440:five_prime_UTR 40.0%
AAGACACCAACCAAACAACT+GGG - chr8_1:87908674-87908693 Msa1223440:five_prime_UTR 40.0%
CATTAACCCAGTTGTTTGGT+TGG + chr8_1:87908683-87908702 None:intergenic 40.0%
CAACCAAACAACTGGGTTAA+TGG - chr8_1:87908681-87908700 Msa1223440:five_prime_UTR 40.0%
ACACTTTCATAGAAGGCCAA+AGG + chr8_1:87907777-87907796 None:intergenic 40.0%
TGTCACACAACCATACCAAT+TGG - chr8_1:87907794-87907813 Msa1223440:three_prime_UTR 40.0%
TGCCACCACTAAGATATCTT+AGG + chr8_1:87907835-87907854 None:intergenic 40.0%
CCTGTTAAGCAAACTATGTG+CGG + chr8_1:87907943-87907962 None:intergenic 40.0%
CATTGAGAGGCTATCTAACA+TGG - chr8_1:87908054-87908073 Msa1223440:CDS 40.0%
GCTTGATGGAGGAGTTAAAA+TGG + chr8_1:87908132-87908151 None:intergenic 40.0%
AGAATTGAGAAGCTTGATGG+AGG + chr8_1:87908143-87908162 None:intergenic 40.0%
!! TGATCTTATCAGCTCTAGGA+AGG + chr8_1:87908399-87908418 None:intergenic 40.0%
!! TTGGCTTTGCATAAAGCACT+TGG + chr8_1:87908605-87908624 None:intergenic 40.0%
! TTGGTGTCTTTCTCTTGCAA+AGG + chr8_1:87908664-87908683 None:intergenic 40.0%
AAAGACACCAACCAAACAAC+TGG - chr8_1:87908673-87908692 Msa1223440:five_prime_UTR 40.0%
AAGACACCAACCAAACAACT+GGG - chr8_1:87908674-87908693 Msa1223440:five_prime_UTR 40.0%
CATTAACCCAGTTGTTTGGT+TGG + chr8_1:87908683-87908702 None:intergenic 40.0%
CAACCAAACAACTGGGTTAA+TGG - chr8_1:87908681-87908700 Msa1223440:five_prime_UTR 40.0%
!! CCGCACATAGTTTGCTTAAC+AGG - chr8_1:87907940-87907959 Msa1223440:three_prime_UTR 45.0%
TTATTATGGTCTAGGCCCCT+AGG - chr8_1:87908464-87908483 Msa1223440:intron 45.0%
! ACCAAGTTGGGCAGATCAAT+GGG - chr8_1:87908505-87908524 Msa1223440:intron 45.0%
! ACAGTTTGAGGCAGTTGCTT+TGG + chr8_1:87908624-87908643 None:intergenic 45.0%
CTGCCTCAAACTGTGTAAAG+TGG - chr8_1:87908630-87908649 Msa1223440:five_prime_UTR 45.0%
CTCCCATTAACCCAGTTGTT+TGG + chr8_1:87908687-87908706 None:intergenic 45.0%
CAAACAACTGGGTTAATGGG+AGG - chr8_1:87908685-87908704 Msa1223440:five_prime_UTR 45.0%
!! CCGCACATAGTTTGCTTAAC+AGG - chr8_1:87907940-87907959 Msa1223440:three_prime_UTR 45.0%
TTATTATGGTCTAGGCCCCT+AGG - chr8_1:87908464-87908483 Msa1223440:intron 45.0%
! ACCAAGTTGGGCAGATCAAT+GGG - chr8_1:87908505-87908524 Msa1223440:intron 45.0%
! ACAGTTTGAGGCAGTTGCTT+TGG + chr8_1:87908624-87908643 None:intergenic 45.0%
CTGCCTCAAACTGTGTAAAG+TGG - chr8_1:87908630-87908649 Msa1223440:five_prime_UTR 45.0%
CTCCCATTAACCCAGTTGTT+TGG + chr8_1:87908687-87908706 None:intergenic 45.0%
CAAACAACTGGGTTAATGGG+AGG - chr8_1:87908685-87908704 Msa1223440:five_prime_UTR 45.0%
!!! TTAATATAGTTTTTTTATTA+TGG - chr8_1:87908450-87908469 Msa1223440:intron 5.0%
!!! TTAATATAGTTTTTTTATTA+TGG - chr8_1:87908450-87908469 Msa1223440:intron 5.0%
TCCGTTGCCATGTTTCCTAG+GGG + chr8_1:87908482-87908501 None:intergenic 50.0%
CTCCGTTGCCATGTTTCCTA+GGG + chr8_1:87908483-87908502 None:intergenic 50.0%
! CACCAAGTTGGGCAGATCAA+TGG - chr8_1:87908504-87908523 Msa1223440:intron 50.0%
! CCAAGTTGGGCAGATCAATG+GGG - chr8_1:87908506-87908525 Msa1223440:CDS 50.0%
TCCGTTGCCATGTTTCCTAG+GGG + chr8_1:87908482-87908501 None:intergenic 50.0%
CTCCGTTGCCATGTTTCCTA+GGG + chr8_1:87908483-87908502 None:intergenic 50.0%
! CACCAAGTTGGGCAGATCAA+TGG - chr8_1:87908504-87908523 Msa1223440:intron 50.0%
! CCAAGTTGGGCAGATCAATG+GGG - chr8_1:87908506-87908525 Msa1223440:CDS 50.0%
GTCTAGGCCCCTAGGAAACA+TGG - chr8_1:87908472-87908491 Msa1223440:intron 55.0%
GCCCCTAGGAAACATGGCAA+CGG - chr8_1:87908478-87908497 Msa1223440:intron 55.0%
CCTCCGTTGCCATGTTTCCT+AGG + chr8_1:87908484-87908503 None:intergenic 55.0%
CCTAGGAAACATGGCAACGG+AGG - chr8_1:87908481-87908500 Msa1223440:intron 55.0%
CCCCATTGATCTGCCCAACT+TGG + chr8_1:87908509-87908528 None:intergenic 55.0%
!!! CGCTGGTGGTTTTGGTGCAA+TGG - chr8_1:87908529-87908548 Msa1223440:CDS 55.0%
GTCTAGGCCCCTAGGAAACA+TGG - chr8_1:87908472-87908491 Msa1223440:intron 55.0%
GCCCCTAGGAAACATGGCAA+CGG - chr8_1:87908478-87908497 Msa1223440:intron 55.0%
CCTCCGTTGCCATGTTTCCT+AGG + chr8_1:87908484-87908503 None:intergenic 55.0%
CCTAGGAAACATGGCAACGG+AGG - chr8_1:87908481-87908500 Msa1223440:intron 55.0%
CCCCATTGATCTGCCCAACT+TGG + chr8_1:87908509-87908528 None:intergenic 55.0%
!!! CGCTGGTGGTTTTGGTGCAA+TGG - chr8_1:87908529-87908548 Msa1223440:CDS 55.0%
!! TGGCAACGGAGGCACCAAGT+TGG - chr8_1:87908492-87908511 Msa1223440:intron 60.0%
!! GGCAACGGAGGCACCAAGTT+GGG - chr8_1:87908493-87908512 Msa1223440:intron 60.0%
!!! CAATGGGGCGCTGGTGGTTT+TGG - chr8_1:87908521-87908540 Msa1223440:CDS 60.0%
!! TGGCAACGGAGGCACCAAGT+TGG - chr8_1:87908492-87908511 Msa1223440:intron 60.0%
!! GGCAACGGAGGCACCAAGTT+GGG - chr8_1:87908493-87908512 Msa1223440:intron 60.0%
!!! CAATGGGGCGCTGGTGGTTT+TGG - chr8_1:87908521-87908540 Msa1223440:CDS 60.0%
TGGGCAGATCAATGGGGCGC+TGG - chr8_1:87908512-87908531 Msa1223440:CDS 65.0%
!! GCAGATCAATGGGGCGCTGG+TGG - chr8_1:87908515-87908534 Msa1223440:CDS 65.0%
TGGGCAGATCAATGGGGCGC+TGG - chr8_1:87908512-87908531 Msa1223440:CDS 65.0%
!! GCAGATCAATGGGGCGCTGG+TGG - chr8_1:87908515-87908534 Msa1223440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 87907734 87908965 87907734 ID=Msa1223440;Name=Msa1223440
chr8_1 mRNA 87907734 87908965 87907734 ID=Msa1223440-mRNA-1;Parent=Msa1223440;Name=Msa1223440-mRNA-1;_AED=0.04;_eAED=0.04;_QI=339|0|0.66|0.66|0|0.33|3|265|141
chr8_1 exon 87907734 87908221 87907734 ID=Msa1223440-mRNA-1:exon:31867;Parent=Msa1223440-mRNA-1
chr8_1 exon 87908276 87908357 87908276 ID=Msa1223440-mRNA-1:exon:31866;Parent=Msa1223440-mRNA-1
chr8_1 exon 87908506 87908965 87908506 ID=Msa1223440-mRNA-1:exon:31865;Parent=Msa1223440-mRNA-1
chr8_1 five_prime_UTR 87908627 87908965 87908627 ID=Msa1223440-mRNA-1:five_prime_utr;Parent=Msa1223440-mRNA-1
chr8_1 CDS 87908506 87908626 87908506 ID=Msa1223440-mRNA-1:cds;Parent=Msa1223440-mRNA-1
chr8_1 CDS 87908276 87908357 87908276 ID=Msa1223440-mRNA-1:cds;Parent=Msa1223440-mRNA-1
chr8_1 CDS 87907999 87908221 87907999 ID=Msa1223440-mRNA-1:cds;Parent=Msa1223440-mRNA-1
chr8_1 three_prime_UTR 87907734 87907998 87907734 ID=Msa1223440-mRNA-1:three_prime_utr;Parent=Msa1223440-mRNA-1
Gene Sequence

>Msa1223440

ATGGTTAGATATATAGCTCCCTATATAGTTATAATTTGGTTCAGTACTCTTTCTGTCCATTTTAACTCCTCCATCAAGCTTCTCAATTCTTCTTCATTCAACTCCAATTCAATCGTTGAAGATAGTATATTTATCAAAGAAATTAATGGTTCAATATTAGAGTATTTTGGTTTCACCTTCCTAGAGCTGATAAGATCAAGATGGCCCCTAGGAAACATGGCAACGGAGGCACCAAGTTGGGCAGATCAATGGGGCGCTGGTGGTTTTGGTGCAATGGAAGATGAGAATACTAGATCGCAAAATGATACAAGCAAGAAGAACAAGAATCCAAGTGCTTTATGCAAAGCCAAAGCAACTGCCTCAAACTGTGTAAAGTGGTTAAAAAGCCTTTGCAAGAGAAAGACACCAACCAAACAACTGGGTTAA
Protein sequence

>Msa1223440

MVRYIAPYIVIIWFSTLSVHFNSSIKLLNSSSFNSNSIVEDSIFIKEINGSILEYFGFTFLELIRSRWPLGNMATEAPSWADQWGAGGFGAMEDENTRSQNDTSKKNKNPSALCKAKATASNCVKWLKSLCKRKTPTKQLG*