AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1234260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234260 MtrunA17_Chr8g0344051 95.755 212 9 0 1 212 1 212 1.22e-147 409
Msa1234260 MtrunA17_Chr8g0344021 67.136 213 68 2 1 212 1 212 5.02e-97 281
Msa1234260 MtrunA17_Chr4g0003361 62.559 211 72 5 3 212 1 205 3.76e-84 248
Msa1234260 MtrunA17_Chr8g0343934 54.245 212 94 2 1 212 1 209 1.57e-79 236
Msa1234260 MtrunA17_Chr8g0344031 57.277 213 88 3 1 212 1 211 1.21e-78 234
Msa1234260 MtrunA17_Chr8g0344041 54.717 212 93 2 1 212 5 213 3.42e-78 233
Msa1234260 MtrunA17_Chr8g0343954 57.143 189 79 2 24 212 23 209 8.52e-75 224
Msa1234260 MtrunA17_Chr2g0301611 52.830 212 97 3 1 212 1 209 1.64e-74 224
Msa1234260 MtrunA17_Chr2g0297231 54.067 209 93 3 1 209 1 206 5.90e-73 219
Msa1234260 MtrunA17_Chr2g0297241 53.555 211 95 3 1 211 1 208 2.00e-72 218
Msa1234260 MtrunA17_Chr7g0217811 57.143 189 79 1 24 212 23 209 3.10e-72 218
Msa1234260 MtrunA17_Chr3g0084531 52.358 212 98 3 1 212 1 209 3.38e-71 215
Msa1234260 MtrunA17_Chr2g0313961 52.885 208 96 2 4 211 3 208 8.84e-71 214
Msa1234260 MtrunA17_Chr3g0115141 52.558 215 94 5 1 212 1 210 2.03e-70 213
Msa1234260 MtrunA17_Chr2g0286171 52.995 217 92 5 1 212 1 212 3.53e-70 213
Msa1234260 MtrunA17_Chr8g0343961 50.239 209 102 2 4 212 3 209 1.51e-69 211
Msa1234260 MtrunA17_Chr4g0012651 56.085 189 81 1 24 212 23 209 1.98e-69 211
Msa1234260 MtrunA17_Chr2g0302541 55.556 189 82 1 24 212 23 209 2.63e-69 210
Msa1234260 MtrunA17_Chr5g0411961 50.000 212 103 3 1 212 1 209 7.19e-69 209
Msa1234260 MtrunA17_Chr2g0302171 50.943 212 101 3 1 212 1 209 7.19e-69 209
Msa1234260 MtrunA17_Chr3g0084511 51.415 212 99 3 1 212 1 208 2.16e-68 208
Msa1234260 MtrunA17_Chr3g0084521 51.887 212 98 3 1 212 1 208 2.41e-68 208
Msa1234260 MtrunA17_Chr8g0343921 50.242 207 99 3 7 212 4 207 5.88e-65 199
Msa1234260 MtrunA17_Chr2g0300581 46.479 213 110 3 1 212 1 210 5.19e-64 197
Msa1234260 MtrunA17_Chr2g0300591 46.009 213 111 3 1 212 1 210 3.90e-63 195
Msa1234260 MtrunA17_Chr2g0300561 46.948 213 109 3 1 212 1 210 1.09e-61 191
Msa1234260 MtrunA17_Chr8g0343911 48.341 211 105 4 3 212 1 208 1.81e-61 191
Msa1234260 MtrunA17_Chr3g0084551 46.602 206 101 3 1 206 1 197 2.36e-52 168
Msa1234260 MtrunA17_Chr6g0449751 41.989 181 86 5 3 170 1 175 7.44e-31 112
Msa1234260 MtrunA17_Chr6g0449791 41.899 179 90 4 3 170 1 176 1.01e-30 112
Msa1234260 MtrunA17_Chr7g0271211 40.110 182 104 4 1 178 2 182 1.12e-30 112
Msa1234260 MtrunA17_Chr6g0449731 41.989 181 86 5 3 170 1 175 1.32e-30 112
Msa1234260 MtrunA17_Chr1g0193671 41.317 167 92 4 7 169 9 173 1.50e-30 112
Msa1234260 MtrunA17_Chr2g0292831 37.245 196 113 4 16 208 19 207 1.70e-30 111
Msa1234260 MtrunA17_Chr6g0449761 41.989 181 86 5 3 170 1 175 1.72e-30 112
Msa1234260 MtrunA17_Chr6g0449771 41.989 181 86 5 3 170 1 175 3.39e-30 111
Msa1234260 MtrunA17_Chr3g0084501 50.909 110 51 2 1 110 1 107 5.28e-30 107
Msa1234260 MtrunA17_Chr1g0193421 37.113 194 118 3 15 205 20 212 6.07e-30 110
Msa1234260 MtrunA17_Chr4g0007031 40.782 179 94 4 1 170 1 176 8.97e-30 110
Msa1234260 MtrunA17_Chr5g0419971 40.782 179 94 4 1 170 1 176 8.97e-30 110
Msa1234260 MtrunA17_Chr5g0419981 40.782 179 94 4 1 170 1 176 8.97e-30 110
Msa1234260 MtrunA17_Chr4g0007051 40.782 179 94 4 1 170 1 176 1.05e-29 109
Msa1234260 MtrunA17_Chr4g0007041 40.223 179 95 4 1 170 1 176 1.21e-29 109
Msa1234260 MtrunA17_Chr2g0292901 44.000 150 77 3 24 170 28 173 1.49e-29 109
Msa1234260 MtrunA17_Chr1g0189841 39.594 197 108 5 1 188 1 195 1.94e-29 109
Msa1234260 MtrunA17_Chr2g0285871 40.782 179 94 4 1 170 1 176 2.99e-29 108
Msa1234260 MtrunA17_Chr1g0156131 39.521 167 91 3 7 170 10 169 5.09e-28 105
Msa1234260 MtrunA17_Chr1g0210761 31.579 209 136 3 4 211 8 210 6.31e-28 105
Msa1234260 MtrunA17_Chr8g0335851 44.030 134 72 2 38 170 47 178 1.55e-27 104
Msa1234260 MtrunA17_Chr3g0130241 36.313 179 103 3 1 170 65 241 1.74e-27 105
Msa1234260 MtrunA17_Chr1g0178711 33.816 207 125 5 11 207 18 222 6.41e-27 102
Msa1234260 MtrunA17_Chr5g0414971 31.937 191 125 4 24 210 32 221 2.08e-26 101
Msa1234260 MtrunA17_Chr6g0449781 36.946 203 115 4 3 195 1 200 1.05e-25 99.4
Msa1234260 MtrunA17_Chr2g0292861 38.596 171 97 4 42 208 7 173 1.88e-25 97.8
Msa1234260 MtrunA17_Chr2g0292911 44.915 118 61 2 42 156 7 123 1.06e-23 92.0
Msa1234260 MtrunA17_Chr2g0292841 43.609 133 70 3 42 170 7 138 1.18e-23 92.8
Msa1234260 MtrunA17_Chr2g0321601 32.386 176 101 7 2 173 7 168 2.70e-17 77.0
Msa1234260 MtrunA17_Chr2g0327651 38.384 99 61 0 46 144 7 105 4.64e-17 73.9
Msa1234260 MtrunA17_Chr4g0026191 32.919 161 93 5 34 180 28 187 1.14e-16 75.1
Msa1234260 MtrunA17_Chr4g0026201 32.919 161 93 5 34 180 28 187 1.68e-16 74.7
Msa1234260 MtrunA17_Chr2g0321581 35.172 145 86 5 34 173 28 169 7.13e-16 73.2
Msa1234260 MtrunA17_Chr2g0321591 34.483 145 87 5 34 173 28 169 1.76e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234260 AT5G20630.1 49.528 212 104 3 1 211 1 210 3.50e-62 193
Msa1234260 AT1G72610.1 49.474 190 94 2 24 212 20 208 1.71e-56 178
Msa1234260 AT5G39160.3 38.636 220 120 6 1 209 1 216 2.90e-39 134
Msa1234260 AT5G39190.1 38.009 221 124 6 1 209 1 220 4.48e-38 132
Msa1234260 AT5G39130.1 38.009 221 124 6 1 209 1 220 4.67e-38 131
Msa1234260 AT5G39160.1 38.009 221 124 6 1 209 1 220 7.42e-38 131
Msa1234260 AT5G38960.1 41.262 206 101 5 20 209 22 223 1.25e-37 130
Msa1234260 AT3G04200.1 37.900 219 125 5 1 209 6 223 2.71e-37 130
Msa1234260 AT5G26700.1 42.328 189 102 5 24 209 26 210 3.41e-36 126
Msa1234260 AT3G05930.1 42.021 188 106 2 24 208 28 215 5.23e-36 126
Msa1234260 AT1G02335.1 38.967 213 118 6 6 209 7 216 3.69e-35 124
Msa1234260 AT5G39110.1 45.395 152 76 3 25 170 30 180 9.84e-35 123
Msa1234260 AT3G62020.1 42.775 173 88 4 9 175 8 175 7.33e-34 120
Msa1234260 AT3G05950.1 46.053 152 76 3 24 170 31 181 1.38e-33 120
Msa1234260 AT3G04150.1 44.079 152 79 3 24 170 31 181 9.19e-33 118
Msa1234260 AT3G04150.2 46.617 133 66 2 42 170 63 194 1.04e-32 118
Msa1234260 AT5G39120.1 40.437 183 90 6 1 170 3 179 1.15e-32 117
Msa1234260 AT5G39180.1 37.755 196 107 4 1 186 5 195 1.64e-32 117
Msa1234260 AT5G39150.1 39.444 180 94 4 1 170 5 179 5.22e-32 116
Msa1234260 AT3G04190.1 44.737 152 78 3 24 170 31 181 1.56e-31 115
Msa1234260 AT5G38940.1 39.583 192 108 4 24 208 31 221 2.04e-31 114
Msa1234260 AT5G38940.2 39.583 192 108 4 24 208 55 245 3.66e-31 114
Msa1234260 AT4G14630.1 45.860 157 70 4 24 170 31 182 6.12e-31 113
Msa1234260 AT1G18980.1 36.893 206 124 4 7 209 17 219 1.04e-30 112
Msa1234260 AT5G39190.2 34.545 220 111 6 1 209 1 198 1.68e-30 111
Msa1234260 AT5G61750.1 38.547 179 94 4 1 170 1 172 2.33e-30 111
Msa1234260 AT5G39160.2 34.545 220 111 6 1 209 1 198 2.87e-30 111
Msa1234260 AT3G62020.2 45.652 138 69 3 39 175 14 146 5.85e-30 110
Msa1234260 AT5G38910.1 37.788 217 117 6 4 208 9 219 2.45e-29 109
Msa1234260 AT5G38930.1 38.542 192 110 4 24 208 31 221 2.71e-29 109
Msa1234260 AT1G09560.1 39.429 175 101 3 16 186 18 191 8.84e-29 107
Msa1234260 AT3G04180.1 36.649 191 113 4 25 208 32 221 3.59e-28 106
Msa1234260 AT1G74820.1 45.522 134 69 3 24 156 41 171 3.82e-28 106
Msa1234260 AT1G18970.1 35.681 213 128 5 3 209 10 219 4.47e-28 105
Msa1234260 AT3G04170.1 42.581 155 74 4 24 170 31 178 3.03e-27 103
Msa1234260 AT1G10460.1 36.000 150 94 2 39 186 42 191 4.02e-26 100
Msa1234260 AT3G10080.1 35.673 171 103 3 41 208 53 219 7.05e-23 92.4
Msa1234260 AT3G10080.2 35.673 171 103 3 41 208 124 290 3.66e-22 92.0
Msa1234260 AT5G38910.2 31.308 214 98 6 4 208 9 182 1.20e-14 69.7

Find 31 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGCTTAAGGTTAATTT+TGG 0.189584 8_2:-11481635 Msa1234260:CDS
CTCTTAATCCTGGTGATGTT+TGG 0.273703 8_2:-11481835 Msa1234260:CDS
TCTTAATCCTGGTGATGTTT+GGG 0.326087 8_2:-11481834 Msa1234260:CDS
GAGTTTGTCGACGTGTTTGC+AGG 0.363951 8_2:+11482058 None:intergenic
TCCACAAATTATGAAGCTTA+AGG 0.372687 8_2:-11481645 Msa1234260:CDS
ATCGTTTCCAGACACCCCTT+CGG 0.376629 8_2:-11482146 Msa1234260:CDS
TCGTTTCCAGACACCCCTTC+GGG 0.386894 8_2:-11482145 Msa1234260:CDS
ACCTTAAGCTTCATAATTTG+TGG 0.389226 8_2:+11481644 None:intergenic
GGATAGCCCGAAGGGGTGTC+TGG 0.391633 8_2:+11482139 None:intergenic
ATCAGAATCAATAGCATTTG+AGG 0.419441 8_2:+11482189 None:intergenic
AGAATAGATCTTGGCTTCAA+TGG 0.435930 8_2:-11481962 Msa1234260:CDS
TTTATACTTAACACTGGTCC+TGG 0.439650 8_2:-11481788 Msa1234260:CDS
TTTATGAAAACTCTTAATCC+TGG 0.452433 8_2:-11481845 Msa1234260:CDS
GAATAAGCAACAGCATTTCC+AGG 0.471369 8_2:+11481770 None:intergenic
TCTGTAGCAAGAATAGATCT+TGG 0.497563 8_2:-11481971 Msa1234260:CDS
ATTCAAGGTCAAATGACTGC+TGG 0.516733 8_2:-11481890 Msa1234260:CDS
ATAAAATGCAAAAGTCCTGT+TGG 0.527650 8_2:+11481806 None:intergenic
GATAAGATACCAGGTCTCAA+TGG 0.527717 8_2:-11482004 Msa1234260:CDS
ATACCAGGTCTCAATGGACT+TGG 0.540360 8_2:-11481998 Msa1234260:CDS
GAATTTATAATCGTCATTCA+AGG 0.543864 8_2:-11481905 Msa1234260:CDS
TTAAGCTTCATAATTTGTGG+AGG 0.583672 8_2:+11481647 None:intergenic
ACTTGCATGGATAGCCCGAA+GGG 0.586847 8_2:+11482131 None:intergenic
GACTTGCATGGATAGCCCGA+AGG 0.590826 8_2:+11482130 None:intergenic
GTACCAATGCACACTCATCC+TGG 0.591016 8_2:-11481935 Msa1234260:CDS
GTAAAAGTCGATAAGATACC+AGG 0.594356 8_2:-11482013 Msa1234260:CDS
ATTATAAATTCGCCTACACC+AGG 0.610124 8_2:+11481917 None:intergenic
ATGCACACTCATCCTGGTGT+AGG 0.618060 8_2:-11481929 Msa1234260:CDS
ACACCAGGATGAGTGTGCAT+TGG 0.622495 8_2:+11481932 None:intergenic
TTCGGGCTATCCATGCAAGT+CGG 0.659661 8_2:-11482128 Msa1234260:CDS
ATACCAAGTCCATTGAGACC+TGG 0.667082 8_2:+11481995 None:intergenic
CTTGCATGGATAGCCCGAAG+GGG 0.715273 8_2:+11482132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAACTGATTTTTTGTTATT+TGG - chr8_2:11482144-11482163 Msa1234260:CDS 15.0%
!!! GTTTTCATAAAAACTTTTGT+AGG + chr8_2:11482006-11482025 None:intergenic 20.0%
! GAATTTATAATCGTCATTCA+AGG - chr8_2:11481955-11481974 Msa1234260:CDS 25.0%
! TTTATGAAAACTCTTAATCC+TGG - chr8_2:11482015-11482034 Msa1234260:CDS 25.0%
!! TTGCATTTTATACTTAACAC+TGG - chr8_2:11482066-11482085 Msa1234260:CDS 25.0%
!! ATGAAGCTTAAGGTTAATTT+TGG - chr8_2:11482225-11482244 Msa1234260:CDS 25.0%
ATCAGAATCAATAGCATTTG+AGG + chr8_2:11481674-11481693 None:intergenic 30.0%
!!! TATCTTATCGACTTTTACAG+TGG + chr8_2:11481845-11481864 None:intergenic 30.0%
ATAAAATGCAAAAGTCCTGT+TGG + chr8_2:11482057-11482076 None:intergenic 30.0%
! TTAAGCTTCATAATTTGTGG+AGG + chr8_2:11482216-11482235 None:intergenic 30.0%
TCCACAAATTATGAAGCTTA+AGG - chr8_2:11482215-11482234 Msa1234260:CDS 30.0%
ACCTTAAGCTTCATAATTTG+TGG + chr8_2:11482219-11482238 None:intergenic 30.0%
! AAGCTTAAGGTTAATTTTGG+TGG - chr8_2:11482228-11482247 Msa1234260:CDS 30.0%
! GCTAATGATTTTGTGTTCTC+TGG - chr8_2:11481769-11481788 Msa1234260:CDS 35.0%
GTAAAAGTCGATAAGATACC+AGG - chr8_2:11481847-11481866 Msa1234260:CDS 35.0%
TCTGTAGCAAGAATAGATCT+TGG - chr8_2:11481889-11481908 Msa1234260:CDS 35.0%
AGAATAGATCTTGGCTTCAA+TGG - chr8_2:11481898-11481917 Msa1234260:CDS 35.0%
ATTATAAATTCGCCTACACC+AGG + chr8_2:11481946-11481965 None:intergenic 35.0%
! TCTTAATCCTGGTGATGTTT+GGG - chr8_2:11482026-11482045 Msa1234260:CDS 35.0%
TTTATACTTAACACTGGTCC+TGG - chr8_2:11482072-11482091 Msa1234260:CDS 35.0%
! AAGAGTAGTCTTTTGAACTG+TGG + chr8_2:11482187-11482206 None:intergenic 35.0%
!! ACATTGTTTTCCGACTTGCA+TGG + chr8_2:11481745-11481764 None:intergenic 40.0%
GATAAGATACCAGGTCTCAA+TGG - chr8_2:11481856-11481875 Msa1234260:CDS 40.0%
ATTCAAGGTCAAATGACTGC+TGG - chr8_2:11481970-11481989 Msa1234260:CDS 40.0%
! CTCTTAATCCTGGTGATGTT+TGG - chr8_2:11482025-11482044 Msa1234260:CDS 40.0%
!! GATGTTTGGGTTTTTCCAAC+AGG - chr8_2:11482039-11482058 Msa1234260:CDS 40.0%
GAATAAGCAACAGCATTTCC+AGG + chr8_2:11482093-11482112 None:intergenic 40.0%
! GTCTTTTGAACTGTGGAAGT+TGG + chr8_2:11482180-11482199 None:intergenic 40.0%
!! AAGGTTAATTTTGGTGGCAG+TGG - chr8_2:11482234-11482253 Msa1234260:CDS 40.0%
! ATACCAGGTCTCAATGGACT+TGG - chr8_2:11481862-11481881 Msa1234260:CDS 45.0%
ATACCAAGTCCATTGAGACC+TGG + chr8_2:11481868-11481887 None:intergenic 45.0%
GGAAAAACCCAAACATCACC+AGG + chr8_2:11482036-11482055 None:intergenic 45.0%
ATCGTTTCCAGACACCCCTT+CGG - chr8_2:11481714-11481733 Msa1234260:CDS 50.0%
ACTTGCATGGATAGCCCGAA+GGG + chr8_2:11481732-11481751 None:intergenic 50.0%
TTCGGGCTATCCATGCAAGT+CGG - chr8_2:11481732-11481751 Msa1234260:CDS 50.0%
GAGTTTGTCGACGTGTTTGC+AGG + chr8_2:11481805-11481824 None:intergenic 50.0%
GTACCAATGCACACTCATCC+TGG - chr8_2:11481925-11481944 Msa1234260:CDS 50.0%
ACACCAGGATGAGTGTGCAT+TGG + chr8_2:11481931-11481950 None:intergenic 50.0%
ATGCACACTCATCCTGGTGT+AGG - chr8_2:11481931-11481950 Msa1234260:CDS 50.0%
TCGTTTCCAGACACCCCTTC+GGG - chr8_2:11481715-11481734 Msa1234260:CDS 55.0%
CTTGCATGGATAGCCCGAAG+GGG + chr8_2:11481731-11481750 None:intergenic 55.0%
GACTTGCATGGATAGCCCGA+AGG + chr8_2:11481733-11481752 None:intergenic 55.0%
!! GGATAGCCCGAAGGGGTGTC+TGG + chr8_2:11481724-11481743 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 11481622 11482260 11481622 ID=Msa1234260;Name=Msa1234260
chr8_2 mRNA 11481622 11482260 11481622 ID=Msa1234260-mRNA-1;Parent=Msa1234260;Name=Msa1234260-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|212
chr8_2 exon 11481622 11482260 11481622 ID=Msa1234260-mRNA-1:exon:4464;Parent=Msa1234260-mRNA-1
chr8_2 CDS 11481622 11482260 11481622 ID=Msa1234260-mRNA-1:cds;Parent=Msa1234260-mRNA-1
Gene Sequence

>Msa1234260

ATGAAGATGATTCAAACTATTCTTTTCTTTTTTGCTCTTATCTCATATACCTCAAATGCTATTGATTCTGATGATTTCTGTGTAGCAGACTTATCGTTTCCAGACACCCCTTCGGGCTATCCATGCAAGTCGGAAAACAATGTAACAGCTAATGATTTTGTGTTCTCTGGCTTAAATATACCTGCAAACACGTCGACAAACTCTTTTAAAGTTGCATTTACCACTGTAAAAGTCGATAAGATACCAGGTCTCAATGGACTTGGTATCTCTGTAGCAAGAATAGATCTTGGCTTCAATGGAAGTGTACCAATGCACACTCATCCTGGTGTAGGCGAATTTATAATCGTCATTCAAGGTCAAATGACTGCTGGATTTATCACACCTACAAAAGTTTTTATGAAAACTCTTAATCCTGGTGATGTTTGGGTTTTTCCAACAGGACTTTTGCATTTTATACTTAACACTGGTCCTGGAAATGCTGTTGCTTATTCTGCATATGCTAGCTCAAATTTTAACACTCATTTAACTGATTTTTTGTTATTTGGTAACAACTTACCAACTTCCACAGTTCAAAAGACTACTCTTCTTGATCCTCCACAAATTATGAAGCTTAAGGTTAATTTTGGTGGCAGTGGCTAG

Protein sequence

>Msa1234260

MKMIQTILFFFALISYTSNAIDSDDFCVADLSFPDTPSGYPCKSENNVTANDFVFSGLNIPANTSTNSFKVAFTTVKVDKIPGLNGLGISVARIDLGFNGSVPMHTHPGVGEFIIVIQGQMTAGFITPTKVFMKTLNPGDVWVFPTGLLHFILNTGPGNAVAYSAYASSNFNTHLTDFLLFGNNLPTSTVQKTTLLDPPQIMKLKVNFGGSG*