AlfalfaGEDB | Msa1234280

M. sativa cultivar zhongmu-4 / Msa1234280

KEGG: -
GO: GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
PFAM: PF00190.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234280	XP_003627289.1	95.755	212	9	0	1	212	1	212	1.32e-143	409

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234280	sp\|O04011\|ABP20_PRUPE	49.524	210	103	3	3	211	6	213	1.60e-61	194

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0036430	Msa1234280	0.817621	2.998156e-52	-8.615850e-47
Msa0084500	Msa1234280	0.805396	1.362058e-49	-8.615850e-47
Msa0176470	Msa1234280	0.807221	5.620372e-50	-8.615850e-47
Msa0236110	Msa1234280	0.801231	9.924669e-49	-8.615850e-47
Msa0322830	Msa1234280	0.802448	5.583660e-49	-8.615850e-47
Msa0742180	Msa1234280	0.805163	1.524386e-49	-8.615850e-47
Msa1169990	Msa1234280	0.815039	1.134078e-51	-8.615850e-47
Msa1201110	Msa1234280	0.816521	5.299165e-52	-8.615850e-47
Msa1201160	Msa1234280	0.803703	3.071633e-49	-8.615850e-47
Msa1234260	Msa1234280	0.939848	6.364545e-100	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234280	MtrunA17_Chr8g0344051	95.755	212	9	0	1	212	1	212	1.22e-147	409
Msa1234280	MtrunA17_Chr8g0344021	67.136	213	68	2	1	212	1	212	5.02e-97	281
Msa1234280	MtrunA17_Chr4g0003361	62.559	211	72	5	3	212	1	205	3.76e-84	248
Msa1234280	MtrunA17_Chr8g0343934	54.245	212	94	2	1	212	1	209	1.57e-79	236
Msa1234280	MtrunA17_Chr8g0344031	57.277	213	88	3	1	212	1	211	1.21e-78	234
Msa1234280	MtrunA17_Chr8g0344041	54.717	212	93	2	1	212	5	213	3.42e-78	233
Msa1234280	MtrunA17_Chr8g0343954	57.143	189	79	2	24	212	23	209	8.52e-75	224
Msa1234280	MtrunA17_Chr2g0301611	52.830	212	97	3	1	212	1	209	1.64e-74	224
Msa1234280	MtrunA17_Chr2g0297231	54.067	209	93	3	1	209	1	206	5.90e-73	219
Msa1234280	MtrunA17_Chr2g0297241	53.555	211	95	3	1	211	1	208	2.00e-72	218

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234280	AT5G20630.1	49.528	212	104	3	1	211	1	210	3.50e-62	193
Msa1234280	AT1G72610.1	49.474	190	94	2	24	212	20	208	1.71e-56	178
Msa1234280	AT5G39160.3	38.636	220	120	6	1	209	1	216	2.90e-39	134
Msa1234280	AT5G39190.1	38.009	221	124	6	1	209	1	220	4.48e-38	132
Msa1234280	AT5G39130.1	38.009	221	124	6	1	209	1	220	4.67e-38	131
Msa1234280	AT5G39160.1	38.009	221	124	6	1	209	1	220	7.42e-38	131
Msa1234280	AT5G38960.1	41.262	206	101	5	20	209	22	223	1.25e-37	130
Msa1234280	AT3G04200.1	37.900	219	125	5	1	209	6	223	2.71e-37	130
Msa1234280	AT5G26700.1	42.328	189	102	5	24	209	26	210	3.41e-36	126
Msa1234280	AT3G05930.1	42.021	188	106	2	24	208	28	215	5.23e-36	126

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Find 31 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTAAGCTTCATAATTTG+TGG	0.389226	8_2:+11486054	None:intergenic
ACGCCAGGATGAGTGTGCAT+TGG	0.618287	8_2:+11486342	None:intergenic
ACTTAATCCTGGTGATGTTT+GGG	0.337157	8_2:-11486244	Msa1234280:CDS
ACTTGCATGGATAGCCCGAA+GGG	0.586847	8_2:+11486541	None:intergenic
AGAATAGATCTTGGCTTCAA+TGG	0.435930	8_2:-11486372	Msa1234280:CDS
ATAAAATGCAAAAGTCCTGT+TGG	0.527650	8_2:+11486216	None:intergenic
ATACCAAGTCCATTGAGACC+TGG	0.667082	8_2:+11486405	None:intergenic
ATACCAGGTCTCAATGGACT+TGG	0.540360	8_2:-11486408	Msa1234280:CDS
ATCAGAATCAATAGCATTTG+AGG	0.419441	8_2:+11486599	None:intergenic
ATCGTTTCCAGACACCCCTT+CGG	0.376629	8_2:-11486556	Msa1234280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAGAATCAATAGCATTTG+AGG	+	chr8_2:11486084-11486103	None:intergenic	30.0%
ATAAAATGCAAAAGTCCTGT+TGG	+	chr8_2:11486467-11486486	None:intergenic	30.0%
TCCACAAATTATGAAGCTTA+AGG	-	chr8_2:11486625-11486644	Msa1234280:CDS	30.0%
ACCTTAAGCTTCATAATTTG+TGG	+	chr8_2:11486629-11486648	None:intergenic	30.0%
GTAAAAGTCGATAAGATACC+AGG	-	chr8_2:11486257-11486276	Msa1234280:CDS	35.0%
TCTGTAGCAAGAATAGATCT+TGG	-	chr8_2:11486299-11486318	Msa1234280:CDS	35.0%
AGAATAGATCTTGGCTTCAA+TGG	-	chr8_2:11486308-11486327	Msa1234280:CDS	35.0%
TTTATACTTAACACTGGTCC+TGG	-	chr8_2:11486482-11486501	Msa1234280:CDS	35.0%
GATAAGATACCAGGTCTCAA+TGG	-	chr8_2:11486266-11486285	Msa1234280:CDS	40.0%
ATTATAAATTCGCCTACGCC+AGG	+	chr8_2:11486356-11486375	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	11486032	11486670	11486032	ID=Msa1234280;Name=Msa1234280
chr8_2	mRNA	11486032	11486670	11486032	ID=Msa1234280-mRNA-1;Parent=Msa1234280;Name=Msa1234280-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|212
chr8_2	exon	11486032	11486670	11486032	ID=Msa1234280-mRNA-1:exon:4465;Parent=Msa1234280-mRNA-1
chr8_2	CDS	11486032	11486670	11486032	ID=Msa1234280-mRNA-1:cds;Parent=Msa1234280-mRNA-1

Gene Sequence

>Msa1234280

ATGAAGATGATTCAAACTATTCTTTTCTTTTTTGCTCTTATCTCATATACCTCAAATGCTATTGATTCTGATGATTTCTGTGTAGCAGACTTATCGTTTCCAGACACCCCTTCGGGCTATCCATGCAAGTCGGAAAACAATGTAACAGCTAATGATTTTGTGTTCTCTGGCTTAAATATACCTGCAAACACGTCGACAAACTCTTTTAAAGTTGCATTTACCACTGTAAAAGTCGATAAGATACCAGGTCTCAATGGACTTGGTATCTCTGTAGCAAGAATAGATCTTGGCTTCAATGGAAGTGTACCAATGCACACTCATCCTGGCGTAGGCGAATTTATAATCGTCATTCAAGGTCAAATGACTGCTGGATTTATCACACCTACAAAAGTTTTTATGAAAACACTTAATCCTGGTGATGTTTGGGTTTTTCCAACAGGACTTTTGCATTTTATACTTAACACTGGTCCTGGAAATGCTGTTGCTTATTCTGCATATGCTAGCTCAAATTTTAACACTCATTTAACTGATTTTTTGTTATTTGGTAACAACTTACCAACTTCCACAGTTCAAAAGACTACTCTTCTTGATCCTCCACAAATTATGAAGCTTAAGGTTAATTTTGGTGGCAGTGGCTAG

Protein sequence

>Msa1234280

MKMIQTILFFFALISYTSNAIDSDDFCVADLSFPDTPSGYPCKSENNVTANDFVFSGLNIPANTSTNSFKVAFTTVKVDKIPGLNGLGISVARIDLGFNGSVPMHTHPGVGEFIIVIQGQMTAGFITPTKVFMKTLNPGDVWVFPTGLLHFILNTGPGNAVAYSAYASSNFNTHLTDFLLFGNNLPTSTVQKTTLLDPPQIMKLKVNFGGSG*