AlfalfaGEDB | Msa1258930

M. sativa cultivar zhongmu-4 / Msa1258930

KEGG: K11294
GO: GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009266 GO:0009409 GO:0009451 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009631 GO:0009892 GO:0009894 GO:0009895 GO:0009941 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0016020 GO:0016070 GO:0016553 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0034641 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043487 GO:0043489 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0055035 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902369
PFAM: PF00076.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258930	KEH31744.1	90.385	104	10	0	91	194	1	104	6.05e-63	201

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258930	sp\|Q9FGS0\|CP31B_ARATH	49.068	161	77	3	52	210	114	271	1.47e-42	148

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258930	A0A072UPH6	90.385	104	10	0	91	194	1	104	2.89e-63	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1258930	Msa1418610	0.859585	3.735052e-63	-8.615850e-47
Msa1258930	Msa1418620	0.860229	2.389016e-63	-8.615850e-47
Msa1208620	Msa1258930	0.958581	1.654197e-116	-8.615850e-47
Msa1208740	Msa1258930	0.937081	6.173269e-98	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258930	MtrunA17_Chr4g0058141	90.385	104	10	0	91	194	1	104	5.56e-67	201
Msa1258930	MtrunA17_Chr4g0058151	53.179	173	75	5	51	220	122	291	6.60e-48	159
Msa1258930	MtrunA17_Chr3g0116631	50.307	163	75	4	52	210	110	270	1.29e-44	150
Msa1258930	MtrunA17_Chr3g0087481	34.146	164	102	3	50	208	59	221	1.14e-20	86.7
Msa1258930	MtrunA17_Chr1g0177101	30.539	167	102	5	52	209	109	270	8.08e-19	83.2
Msa1258930	MtrunA17_Chr7g0267571	30.726	179	113	6	52	220	110	287	1.29e-16	77.0
Msa1258930	MtrunA17_Chr1g0181111	35.227	176	95	4	52	209	85	259	1.73e-15	73.6
Msa1258930	MtrunA17_Chr1g0168601	32.759	116	72	2	92	207	105	214	2.35e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1258930	AT5G50250.1	49.068	161	77	3	52	210	114	271	1.50e-43	148
Msa1258930	AT4G24770.1	48.447	161	78	3	52	210	151	308	1.42e-40	141
Msa1258930	AT4G24770.2	48.447	161	78	3	52	210	151	308	1.42e-40	141
Msa1258930	AT2G37220.1	39.674	184	79	5	51	209	91	267	1.88e-25	100
Msa1258930	AT3G52150.2	34.359	195	113	7	21	208	53	239	7.61e-20	85.5
Msa1258930	AT3G52150.1	34.359	195	113	7	21	208	53	239	7.61e-20	85.5
Msa1258930	AT3G52380.1	30.108	186	105	6	52	220	117	294	2.26e-19	85.1
Msa1258930	AT2G35410.1	32.941	170	105	5	52	214	96	263	1.86e-18	82.4
Msa1258930	AT1G60000.1	31.875	160	102	4	52	209	86	240	1.61e-12	65.5

Find 61 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATAGCATTTGAAATAAT+AGG	0.207293	8_2:+60237796	None:intergenic
TCAAATGCGCGTGGCCGTTT+TGG	0.211966	8_2:+60237016	None:intergenic
TGAGTAACATAGACACTAAA+AGG	0.317271	8_2:+60236989	None:intergenic
ATGGCGGGTTCTTCTCCTTC+TGG	0.342108	8_2:+60237699	None:intergenic
ATTAATTTCTTACTTATCAT+TGG	0.344341	8_2:-60236738	Msa1258930:three_prime_UTR
TTCTTCTCTTCTCACATTTG+CGG	0.355015	8_2:-60237677	Msa1258930:CDS
TTGGTGTGCAAGAGTGAATT+AGG	0.356166	8_2:+60237829	None:intergenic
AATAATAGGGAATGTGGTTT+TGG	0.357319	8_2:+60237810	None:intergenic
TAATAGCATTTGAAATAATA+GGG	0.362798	8_2:+60237797	None:intergenic
CTTCACGGTCAGGTAAATAT+TGG	0.385055	8_2:-60236800	Msa1258930:CDS
CTAGCATTGTCTTGTGGATA+AGG	0.388661	8_2:+60237979	None:intergenic
TGGACACAATACGAAATGTT+TGG	0.388770	8_2:-60237230	Msa1258930:CDS
AATGTCATTGCTGCTCTTCA+CGG	0.407509	8_2:-60236815	Msa1258930:CDS
CATCATATGATAATTCTTCA+AGG	0.413977	8_2:-60237747	Msa1258930:CDS
AAATTTCTTGACTGTTAGTA+AGG	0.416600	8_2:-60237068	Msa1258930:CDS
TCAGGTCGATCACAGGGTTA+TGG	0.424167	8_2:-60236872	Msa1258930:CDS
GATTTCAGGTAATTTATACT+TGG	0.431344	8_2:-60237250	Msa1258930:intron
TCTCTTCTCACATTTGCGGT+TGG	0.448348	8_2:-60237673	Msa1258930:CDS
GAAGAATTATCATATGATGA+AGG	0.452268	8_2:+60237751	None:intergenic
CATTGCTGCTCTTCACGGTC+AGG	0.452683	8_2:-60236810	Msa1258930:CDS
ATTGGTAACATAACATGTAT+TGG	0.463416	8_2:-60236720	Msa1258930:three_prime_UTR
TTCTTCTGAAGATTTACGTT+GGG	0.466529	8_2:-60237638	Msa1258930:CDS
TTAATCTGTAACAAAGTCTC+AGG	0.469763	8_2:-60236890	Msa1258930:CDS
GAAAGGAACACCACAACACT+AGG	0.478587	8_2:+60237926	None:intergenic
ATTATTCAGCAAACATGGTA+AGG	0.486096	8_2:-60236927	Msa1258930:CDS
GTATTGGTAATTAAATGTAT+TGG	0.495914	8_2:-60236704	None:intergenic
ACATTTGCGGTTGGTGATGT+TGG	0.499859	8_2:-60237664	Msa1258930:CDS
GAGAAGGATGAGAAGGAGAA+AGG	0.504406	8_2:+60237909	None:intergenic
AAGAATTATCATATGATGAA+GGG	0.504905	8_2:+60237752	None:intergenic
AACTTCTTCAACGGTACTCA+TGG	0.505528	8_2:+60237199	None:intergenic
ATGTTGAGAGAAGGATGAGA+AGG	0.505906	8_2:+60237902	None:intergenic
CACTACCTGATCTCCCAAAA+CGG	0.511369	8_2:-60237030	Msa1258930:CDS
ATTTGAAATAATAGGGAATG+TGG	0.513634	8_2:+60237804	None:intergenic
CAAAGTCTCAGGTCGATCAC+AGG	0.513736	8_2:-60236879	Msa1258930:CDS
ATCATGTCTATCACAATGAC+AGG	0.526571	8_2:-60237871	Msa1258930:five_prime_UTR
GACTCAAGATACCGATGTTG+AGG	0.546964	8_2:-60237610	Msa1258930:CDS
CGCTCTCTCTCACCGCCAGA+AGG	0.551040	8_2:-60237714	Msa1258930:CDS
CTAATACTAGCATTGTCTTG+TGG	0.551172	8_2:+60237973	None:intergenic
ATTCTTCTGAAGATTTACGT+TGG	0.551307	8_2:-60237639	Msa1258930:CDS
GAACACCACAACACTAGGGT+TGG	0.551757	8_2:+60237931	None:intergenic
AGCCTTCTCAACTTCTTCAA+CGG	0.556305	8_2:+60237190	None:intergenic
TTTGTGTGACAGGAATTAGC+TGG	0.556926	8_2:-60237091	Msa1258930:intron
AAGGCTATGAAGAAGTTCAA+TGG	0.566594	8_2:-60237173	Msa1258930:CDS
AATTGAATTGATTAAACACG+TGG	0.578559	8_2:+60238028	None:intergenic
GAGCAATTATTCAGCAAACA+TGG	0.587655	8_2:-60236932	Msa1258930:CDS
TACCGTTGAAGAAGTTGAGA+AGG	0.595905	8_2:-60237192	Msa1258930:CDS
TCTGGCGGTGAGAGAGAGCG+AGG	0.599566	8_2:+60237717	None:intergenic
TGTTGAGAGTAATGAACACT+TGG	0.600502	8_2:-60236954	Msa1258930:CDS
TGTGAGAAGAGAAGAATGGC+GGG	0.607125	8_2:+60237684	None:intergenic
GCGGGTTCTTCTCCTTCTGG+CGG	0.609161	8_2:+60237702	None:intergenic
AAAGTCTCAGGTCGATCACA+GGG	0.610446	8_2:-60236878	Msa1258930:CDS
CAAATGTGAGAAGAGAAGAA+TGG	0.634541	8_2:+60237680	None:intergenic
CAAATCCAACCCTAGTGTTG+TGG	0.661373	8_2:-60237936	Msa1258930:five_prime_UTR
CTGGCGGTGAGAGAGAGCGA+GGG	0.661739	8_2:+60237718	None:intergenic
TATAGCTGAATGTTGAGAGA+AGG	0.662316	8_2:+60237893	None:intergenic
AAAGGAACACCACAACACTA+GGG	0.662871	8_2:+60237927	None:intergenic
TCTATGTTACTCAGTTGCCA+CGG	0.664591	8_2:-60236979	Msa1258930:CDS
ATGTGAGAAGAGAAGAATGG+CGG	0.670632	8_2:+60237683	None:intergenic
AAAGGAGAATCAAATGCGCG+TGG	0.672402	8_2:+60237007	None:intergenic
TCATTACTCTCAACACTCCG+TGG	0.683276	8_2:+60236962	None:intergenic
TGGGACTGTAGTGCATGCTG+AGG	0.704678	8_2:-60237562	Msa1258930:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATGAAATAAAAAAAGTTA+AGG	+	chr8_2:60237202-60237221	None:intergenic	10.0%
!!	TAATGAAATAAAAAAAGTTA+AGG	+	chr8_2:60237202-60237221	None:intergenic	10.0%
!!!	TAATAGCATTTGAAATAATA+GGG	+	chr8_2:60236936-60236955	None:intergenic	15.0%
!!!	TTAATAGCATTTGAAATAAT+AGG	+	chr8_2:60236937-60236956	None:intergenic	15.0%
!!!	AGATTATGTGTAATATATTT+TGG	-	chr8_2:60237225-60237244	Msa1258930:CDS	15.0%
!!!	TTAACTTTTTTCATTTTCAA+TGG	+	chr8_2:60237411-60237430	None:intergenic	15.0%
!!	ATTAATTTCTTACTTATCAT+TGG	-	chr8_2:60237992-60238011	Msa1258930:five_prime_UTR	15.0%
!!!	TAATAGCATTTGAAATAATA+GGG	+	chr8_2:60236936-60236955	None:intergenic	15.0%
!!!	TTAATAGCATTTGAAATAAT+AGG	+	chr8_2:60236937-60236956	None:intergenic	15.0%
!!!	AGATTATGTGTAATATATTT+TGG	-	chr8_2:60237225-60237244	Msa1258930:CDS	15.0%
!!!	TTAACTTTTTTCATTTTCAA+TGG	+	chr8_2:60237411-60237430	None:intergenic	15.0%
!!	ATTAATTTCTTACTTATCAT+TGG	-	chr8_2:60237992-60238011	Msa1258930:five_prime_UTR	15.0%
!!	AAGAATTATCATATGATGAA+GGG	+	chr8_2:60236981-60237000	None:intergenic	20.0%
!!!	TAGACTTAACCATTTTATTA+GGG	+	chr8_2:60237353-60237372	None:intergenic	20.0%
!!!	ATAGACTTAACCATTTTATT+AGG	+	chr8_2:60237354-60237373	None:intergenic	20.0%
!!!	TACCTGAAATCATTTTATTA+GGG	+	chr8_2:60237471-60237490	None:intergenic	20.0%
!!!	TTACCTGAAATCATTTTATT+AGG	+	chr8_2:60237472-60237491	None:intergenic	20.0%
!!	TACACTACAAAAATATAAAC+AGG	+	chr8_2:60237596-60237615	None:intergenic	20.0%
!!	AAGAATTATCATATGATGAA+GGG	+	chr8_2:60236981-60237000	None:intergenic	20.0%
!!!	TAGACTTAACCATTTTATTA+GGG	+	chr8_2:60237353-60237372	None:intergenic	20.0%
!!!	ATAGACTTAACCATTTTATT+AGG	+	chr8_2:60237354-60237373	None:intergenic	20.0%
!!!	TACCTGAAATCATTTTATTA+GGG	+	chr8_2:60237471-60237490	None:intergenic	20.0%
!!!	TTACCTGAAATCATTTTATT+AGG	+	chr8_2:60237472-60237491	None:intergenic	20.0%
!!	TACACTACAAAAATATAAAC+AGG	+	chr8_2:60237596-60237615	None:intergenic	20.0%
!!	ATTTGAAATAATAGGGAATG+TGG	+	chr8_2:60236929-60236948	None:intergenic	25.0%
!	GAAGAATTATCATATGATGA+AGG	+	chr8_2:60236982-60237001	None:intergenic	25.0%
!	CATCATATGATAATTCTTCA+AGG	-	chr8_2:60236983-60237002	Msa1258930:CDS	25.0%
!!!	AGGAATTGAAAACACTTTTT+GGG	-	chr8_2:60237140-60237159	Msa1258930:intron	25.0%
!	ATATGCATTCCCTAATAAAA+TGG	-	chr8_2:60237341-60237360	Msa1258930:intron	25.0%
!	ATGGTCAAATTTATTTGTCA+TGG	+	chr8_2:60237392-60237411	None:intergenic	25.0%
!	TTCCCTAATAAAATGATTTC+AGG	-	chr8_2:60237466-60237485	Msa1258930:intron	25.0%
!!	GATTTCAGGTAATTTATACT+TGG	-	chr8_2:60237480-60237499	Msa1258930:intron	25.0%
!!	TTTTTGTAGTGTAGTTGTTT+AGG	-	chr8_2:60237603-60237622	Msa1258930:CDS	25.0%
!	AAATTTCTTGACTGTTAGTA+AGG	-	chr8_2:60237662-60237681	Msa1258930:CDS	25.0%
!!	ATTGGTAACATAACATGTAT+TGG	-	chr8_2:60238010-60238029	Msa1258930:five_prime_UTR	25.0%
!!	ATTTGAAATAATAGGGAATG+TGG	+	chr8_2:60236929-60236948	None:intergenic	25.0%
!	GAAGAATTATCATATGATGA+AGG	+	chr8_2:60236982-60237001	None:intergenic	25.0%
!	CATCATATGATAATTCTTCA+AGG	-	chr8_2:60236983-60237002	Msa1258930:CDS	25.0%
!!!	AGGAATTGAAAACACTTTTT+GGG	-	chr8_2:60237140-60237159	Msa1258930:intron	25.0%
!	ATATGCATTCCCTAATAAAA+TGG	-	chr8_2:60237341-60237360	Msa1258930:intron	25.0%
!	ATGGTCAAATTTATTTGTCA+TGG	+	chr8_2:60237392-60237411	None:intergenic	25.0%
!	TTCCCTAATAAAATGATTTC+AGG	-	chr8_2:60237466-60237485	Msa1258930:intron	25.0%
!!	GATTTCAGGTAATTTATACT+TGG	-	chr8_2:60237480-60237499	Msa1258930:intron	25.0%
!!	TTTTTGTAGTGTAGTTGTTT+AGG	-	chr8_2:60237603-60237622	Msa1258930:CDS	25.0%
!	AAATTTCTTGACTGTTAGTA+AGG	-	chr8_2:60237662-60237681	Msa1258930:CDS	25.0%
!!	ATTGGTAACATAACATGTAT+TGG	-	chr8_2:60238010-60238029	Msa1258930:five_prime_UTR	25.0%
!	AATAATAGGGAATGTGGTTT+TGG	+	chr8_2:60236923-60236942	None:intergenic	30.0%
!	ATTCTTCTGAAGATTTACGT+TGG	-	chr8_2:60237091-60237110	Msa1258930:intron	30.0%
!	TTCTTCTGAAGATTTACGTT+GGG	-	chr8_2:60237092-60237111	Msa1258930:intron	30.0%
!	TGTTTTCAATTCCTCAACAT+CGG	+	chr8_2:60237134-60237153	None:intergenic	30.0%
!	GAGGAATTGAAAACACTTTT+TGG	-	chr8_2:60237139-60237158	Msa1258930:intron	30.0%
!	CTTTTGCTTATAGTTTAGTC+AGG	+	chr8_2:60237274-60237293	None:intergenic	30.0%
	TGAGTAACATAGACACTAAA+AGG	+	chr8_2:60237744-60237763	None:intergenic	30.0%
	ATTATTCAGCAAACATGGTA+AGG	-	chr8_2:60237803-60237822	Msa1258930:exon	30.0%
	TTAATCTGTAACAAAGTCTC+AGG	-	chr8_2:60237840-60237859	Msa1258930:five_prime_UTR	30.0%
!	AATAATAGGGAATGTGGTTT+TGG	+	chr8_2:60236923-60236942	None:intergenic	30.0%
!	ATTCTTCTGAAGATTTACGT+TGG	-	chr8_2:60237091-60237110	Msa1258930:intron	30.0%
!	TTCTTCTGAAGATTTACGTT+GGG	-	chr8_2:60237092-60237111	Msa1258930:intron	30.0%
!	TGTTTTCAATTCCTCAACAT+CGG	+	chr8_2:60237134-60237153	None:intergenic	30.0%
!	GAGGAATTGAAAACACTTTT+TGG	-	chr8_2:60237139-60237158	Msa1258930:intron	30.0%
!	CTTTTGCTTATAGTTTAGTC+AGG	+	chr8_2:60237274-60237293	None:intergenic	30.0%
	TGAGTAACATAGACACTAAA+AGG	+	chr8_2:60237744-60237763	None:intergenic	30.0%
	ATTATTCAGCAAACATGGTA+AGG	-	chr8_2:60237803-60237822	Msa1258930:exon	30.0%
	TTAATCTGTAACAAAGTCTC+AGG	-	chr8_2:60237840-60237859	Msa1258930:five_prime_UTR	30.0%
	CTAATACTAGCATTGTCTTG+TGG	+	chr8_2:60236760-60236779	None:intergenic	35.0%
	TATAGCTGAATGTTGAGAGA+AGG	+	chr8_2:60236840-60236859	None:intergenic	35.0%
	ATCATGTCTATCACAATGAC+AGG	-	chr8_2:60236859-60236878	Msa1258930:CDS	35.0%
	CAAATGTGAGAAGAGAAGAA+TGG	+	chr8_2:60237053-60237072	None:intergenic	35.0%
	TTCTTCTCTTCTCACATTTG+CGG	-	chr8_2:60237053-60237072	Msa1258930:CDS	35.0%
!!	ATTGAAAACACTTTTTGGGC+AGG	-	chr8_2:60237144-60237163	Msa1258930:intron	35.0%
!	ACGCAGTACAAAATGTTTTC+AGG	-	chr8_2:60237439-60237458	Msa1258930:intron	35.0%
	TGGACACAATACGAAATGTT+TGG	-	chr8_2:60237500-60237519	Msa1258930:intron	35.0%
	AAGGCTATGAAGAAGTTCAA+TGG	-	chr8_2:60237557-60237576	Msa1258930:intron	35.0%
	TAATTCATCCTTTGTGTGAC+AGG	-	chr8_2:60237629-60237648	Msa1258930:CDS	35.0%
	TGTTGAGAGTAATGAACACT+TGG	-	chr8_2:60237776-60237795	Msa1258930:CDS	35.0%
	GAGCAATTATTCAGCAAACA+TGG	-	chr8_2:60237798-60237817	Msa1258930:exon	35.0%
	CTAATACTAGCATTGTCTTG+TGG	+	chr8_2:60236760-60236779	None:intergenic	35.0%
	TATAGCTGAATGTTGAGAGA+AGG	+	chr8_2:60236840-60236859	None:intergenic	35.0%
	ATCATGTCTATCACAATGAC+AGG	-	chr8_2:60236859-60236878	Msa1258930:CDS	35.0%
	CAAATGTGAGAAGAGAAGAA+TGG	+	chr8_2:60237053-60237072	None:intergenic	35.0%
	TTCTTCTCTTCTCACATTTG+CGG	-	chr8_2:60237053-60237072	Msa1258930:CDS	35.0%
!!	ATTGAAAACACTTTTTGGGC+AGG	-	chr8_2:60237144-60237163	Msa1258930:intron	35.0%
!	ACGCAGTACAAAATGTTTTC+AGG	-	chr8_2:60237439-60237458	Msa1258930:intron	35.0%
	TGGACACAATACGAAATGTT+TGG	-	chr8_2:60237500-60237519	Msa1258930:intron	35.0%
	AAGGCTATGAAGAAGTTCAA+TGG	-	chr8_2:60237557-60237576	Msa1258930:intron	35.0%
	TAATTCATCCTTTGTGTGAC+AGG	-	chr8_2:60237629-60237648	Msa1258930:CDS	35.0%
	TGTTGAGAGTAATGAACACT+TGG	-	chr8_2:60237776-60237795	Msa1258930:CDS	35.0%
	GAGCAATTATTCAGCAAACA+TGG	-	chr8_2:60237798-60237817	Msa1258930:exon	35.0%
	CTAGCATTGTCTTGTGGATA+AGG	+	chr8_2:60236754-60236773	None:intergenic	40.0%
	AAAGGAACACCACAACACTA+GGG	+	chr8_2:60236806-60236825	None:intergenic	40.0%
	ATGTTGAGAGAAGGATGAGA+AGG	+	chr8_2:60236831-60236850	None:intergenic	40.0%
	TTGGTGTGCAAGAGTGAATT+AGG	+	chr8_2:60236904-60236923	None:intergenic	40.0%
	ATGTGAGAAGAGAAGAATGG+CGG	+	chr8_2:60237050-60237069	None:intergenic	40.0%
	AACTTCTTCAACGGTACTCA+TGG	+	chr8_2:60237534-60237553	None:intergenic	40.0%
	TACCGTTGAAGAAGTTGAGA+AGG	-	chr8_2:60237538-60237557	Msa1258930:intron	40.0%
	AGCCTTCTCAACTTCTTCAA+CGG	+	chr8_2:60237543-60237562	None:intergenic	40.0%
	AGCTAATTCCTGTCACACAA+AGG	+	chr8_2:60237640-60237659	None:intergenic	40.0%
	TTTGTGTGACAGGAATTAGC+TGG	-	chr8_2:60237639-60237658	Msa1258930:CDS	40.0%
	TCTATGTTACTCAGTTGCCA+CGG	-	chr8_2:60237751-60237770	Msa1258930:CDS	40.0%
	AATGTCATTGCTGCTCTTCA+CGG	-	chr8_2:60237915-60237934	Msa1258930:five_prime_UTR	40.0%
	CTTCACGGTCAGGTAAATAT+TGG	-	chr8_2:60237930-60237949	Msa1258930:five_prime_UTR	40.0%
	CTAGCATTGTCTTGTGGATA+AGG	+	chr8_2:60236754-60236773	None:intergenic	40.0%
	AAAGGAACACCACAACACTA+GGG	+	chr8_2:60236806-60236825	None:intergenic	40.0%
	ATGTTGAGAGAAGGATGAGA+AGG	+	chr8_2:60236831-60236850	None:intergenic	40.0%
	TTGGTGTGCAAGAGTGAATT+AGG	+	chr8_2:60236904-60236923	None:intergenic	40.0%
	ATGTGAGAAGAGAAGAATGG+CGG	+	chr8_2:60237050-60237069	None:intergenic	40.0%
	AACTTCTTCAACGGTACTCA+TGG	+	chr8_2:60237534-60237553	None:intergenic	40.0%
	TACCGTTGAAGAAGTTGAGA+AGG	-	chr8_2:60237538-60237557	Msa1258930:intron	40.0%
	AGCCTTCTCAACTTCTTCAA+CGG	+	chr8_2:60237543-60237562	None:intergenic	40.0%
	AGCTAATTCCTGTCACACAA+AGG	+	chr8_2:60237640-60237659	None:intergenic	40.0%
	TTTGTGTGACAGGAATTAGC+TGG	-	chr8_2:60237639-60237658	Msa1258930:CDS	40.0%
	TCTATGTTACTCAGTTGCCA+CGG	-	chr8_2:60237751-60237770	Msa1258930:CDS	40.0%
	AATGTCATTGCTGCTCTTCA+CGG	-	chr8_2:60237915-60237934	Msa1258930:five_prime_UTR	40.0%
	CTTCACGGTCAGGTAAATAT+TGG	-	chr8_2:60237930-60237949	Msa1258930:five_prime_UTR	40.0%
	CAAATCCAACCCTAGTGTTG+TGG	-	chr8_2:60236794-60236813	Msa1258930:CDS	45.0%
	GAAAGGAACACCACAACACT+AGG	+	chr8_2:60236807-60236826	None:intergenic	45.0%
	GAGAAGGATGAGAAGGAGAA+AGG	+	chr8_2:60236824-60236843	None:intergenic	45.0%
!!	GGGTTTTGTAGCTGCTTGAA+AGG	+	chr8_2:60236961-60236980	None:intergenic	45.0%
	TGTGAGAAGAGAAGAATGGC+GGG	+	chr8_2:60237049-60237068	None:intergenic	45.0%
	TCTCTTCTCACATTTGCGGT+TGG	-	chr8_2:60237057-60237076	Msa1258930:CDS	45.0%
!!	ACATTTGCGGTTGGTGATGT+TGG	-	chr8_2:60237066-60237085	Msa1258930:CDS	45.0%
	GACTCAAGATACCGATGTTG+AGG	-	chr8_2:60237120-60237139	Msa1258930:intron	45.0%
!!	AAAACACTTTTTGGGCAGGC+TGG	-	chr8_2:60237148-60237167	Msa1258930:intron	45.0%
!!	AAACACTTTTTGGGCAGGCT+GGG	-	chr8_2:60237149-60237168	Msa1258930:intron	45.0%
	CACTACCTGATCTCCCAAAA+CGG	-	chr8_2:60237700-60237719	Msa1258930:CDS	45.0%
	AAAGGAGAATCAAATGCGCG+TGG	+	chr8_2:60237726-60237745	None:intergenic	45.0%
	TCATTACTCTCAACACTCCG+TGG	+	chr8_2:60237771-60237790	None:intergenic	45.0%
	AAAGTCTCAGGTCGATCACA+GGG	-	chr8_2:60237852-60237871	Msa1258930:five_prime_UTR	45.0%
	CAAATCCAACCCTAGTGTTG+TGG	-	chr8_2:60236794-60236813	Msa1258930:CDS	45.0%
	GAAAGGAACACCACAACACT+AGG	+	chr8_2:60236807-60236826	None:intergenic	45.0%
	GAGAAGGATGAGAAGGAGAA+AGG	+	chr8_2:60236824-60236843	None:intergenic	45.0%
!!	GGGTTTTGTAGCTGCTTGAA+AGG	+	chr8_2:60236961-60236980	None:intergenic	45.0%
	TGTGAGAAGAGAAGAATGGC+GGG	+	chr8_2:60237049-60237068	None:intergenic	45.0%
	TCTCTTCTCACATTTGCGGT+TGG	-	chr8_2:60237057-60237076	Msa1258930:CDS	45.0%
!!	ACATTTGCGGTTGGTGATGT+TGG	-	chr8_2:60237066-60237085	Msa1258930:CDS	45.0%
	GACTCAAGATACCGATGTTG+AGG	-	chr8_2:60237120-60237139	Msa1258930:intron	45.0%
!!	AAAACACTTTTTGGGCAGGC+TGG	-	chr8_2:60237148-60237167	Msa1258930:intron	45.0%
!!	AAACACTTTTTGGGCAGGCT+GGG	-	chr8_2:60237149-60237168	Msa1258930:intron	45.0%
	CACTACCTGATCTCCCAAAA+CGG	-	chr8_2:60237700-60237719	Msa1258930:CDS	45.0%
	AAAGGAGAATCAAATGCGCG+TGG	+	chr8_2:60237726-60237745	None:intergenic	45.0%
	TCATTACTCTCAACACTCCG+TGG	+	chr8_2:60237771-60237790	None:intergenic	45.0%
	AAAGTCTCAGGTCGATCACA+GGG	-	chr8_2:60237852-60237871	Msa1258930:five_prime_UTR	45.0%
	GAACACCACAACACTAGGGT+TGG	+	chr8_2:60236802-60236821	None:intergenic	50.0%
	CAAAGTCTCAGGTCGATCAC+AGG	-	chr8_2:60237851-60237870	Msa1258930:five_prime_UTR	50.0%
	TCAGGTCGATCACAGGGTTA+TGG	-	chr8_2:60237858-60237877	Msa1258930:five_prime_UTR	50.0%
	GAACACCACAACACTAGGGT+TGG	+	chr8_2:60236802-60236821	None:intergenic	50.0%
	CAAAGTCTCAGGTCGATCAC+AGG	-	chr8_2:60237851-60237870	Msa1258930:five_prime_UTR	50.0%
	TCAGGTCGATCACAGGGTTA+TGG	-	chr8_2:60237858-60237877	Msa1258930:five_prime_UTR	50.0%
!!	ATGGCGGGTTCTTCTCCTTC+TGG	+	chr8_2:60237034-60237053	None:intergenic	55.0%
	TGGGACTGTAGTGCATGCTG+AGG	-	chr8_2:60237168-60237187	Msa1258930:intron	55.0%
!!	CAAATGCGCGTGGCCGTTTT+GGG	+	chr8_2:60237716-60237735	None:intergenic	55.0%
!	TCAAATGCGCGTGGCCGTTT+TGG	+	chr8_2:60237717-60237736	None:intergenic	55.0%
	CATTGCTGCTCTTCACGGTC+AGG	-	chr8_2:60237920-60237939	Msa1258930:five_prime_UTR	55.0%
!!	ATGGCGGGTTCTTCTCCTTC+TGG	+	chr8_2:60237034-60237053	None:intergenic	55.0%
	TGGGACTGTAGTGCATGCTG+AGG	-	chr8_2:60237168-60237187	Msa1258930:intron	55.0%
!!	CAAATGCGCGTGGCCGTTTT+GGG	+	chr8_2:60237716-60237735	None:intergenic	55.0%
!	TCAAATGCGCGTGGCCGTTT+TGG	+	chr8_2:60237717-60237736	None:intergenic	55.0%
	CATTGCTGCTCTTCACGGTC+AGG	-	chr8_2:60237920-60237939	Msa1258930:five_prime_UTR	55.0%
	GCGGGTTCTTCTCCTTCTGG+CGG	+	chr8_2:60237031-60237050	None:intergenic	60.0%
!!!	CGTGGCCGTTTTGGGAGATC+AGG	+	chr8_2:60237708-60237727	None:intergenic	60.0%
	GCGGGTTCTTCTCCTTCTGG+CGG	+	chr8_2:60237031-60237050	None:intergenic	60.0%
!!!	CGTGGCCGTTTTGGGAGATC+AGG	+	chr8_2:60237708-60237727	None:intergenic	60.0%
	CTGGCGGTGAGAGAGAGCGA+GGG	+	chr8_2:60237015-60237034	None:intergenic	65.0%
	TCTGGCGGTGAGAGAGAGCG+AGG	+	chr8_2:60237016-60237035	None:intergenic	65.0%
	CGCTCTCTCTCACCGCCAGA+AGG	-	chr8_2:60237016-60237035	Msa1258930:CDS	65.0%
	CTGGCGGTGAGAGAGAGCGA+GGG	+	chr8_2:60237015-60237034	None:intergenic	65.0%
	TCTGGCGGTGAGAGAGAGCG+AGG	+	chr8_2:60237016-60237035	None:intergenic	65.0%
	CGCTCTCTCTCACCGCCAGA+AGG	-	chr8_2:60237016-60237035	Msa1258930:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	60236705	60238047	60236705	ID=Msa1258930;Name=Msa1258930
chr8_2	mRNA	60236705	60238047	60236705	ID=Msa1258930-mRNA-1;Parent=Msa1258930;Name=Msa1258930-mRNA-1;_AED=0.33;_eAED=0.33;_QI=242\|1\|1\|1\|1\|1\|3\|67\|222
chr8_2	exon	60236705	60237101	60236705	ID=Msa1258930-mRNA-1:exon:18914;Parent=Msa1258930-mRNA-1
chr8_2	exon	60237169	60237264	60237169	ID=Msa1258930-mRNA-1:exon:18913;Parent=Msa1258930-mRNA-1
chr8_2	exon	60237563	60238047	60237563	ID=Msa1258930-mRNA-1:exon:18912;Parent=Msa1258930-mRNA-1
chr8_2	five_prime_UTR	60237806	60238047	60237806	ID=Msa1258930-mRNA-1:five_prime_utr;Parent=Msa1258930-mRNA-1
chr8_2	CDS	60237563	60237805	60237563	ID=Msa1258930-mRNA-1:cds;Parent=Msa1258930-mRNA-1
chr8_2	CDS	60237169	60237264	60237169	ID=Msa1258930-mRNA-1:cds;Parent=Msa1258930-mRNA-1
chr8_2	CDS	60236772	60237101	60236772	ID=Msa1258930-mRNA-1:cds;Parent=Msa1258930-mRNA-1
chr8_2	three_prime_UTR	60236705	60236771	60236705	ID=Msa1258930-mRNA-1:three_prime_utr;Parent=Msa1258930-mRNA-1

Gene Sequence

>Msa1258930

ATGCTATTAAGCCTTTCAAGCAGCTACAAAACCCTTCATCATATGATAATTCTTCAAGGTTTTCGCCCTCGCTCTCTCTCACCGCCAGAAGGAGAAGAACCCGCCATTCTTCTCTTCTCACATTTGCGGTTGGTGATGTTGGAAATTCTTCTGAAGATTTACGTTGGGAATTTGACTCAAGATACCGATGTTGAGGAATTGAAAACACTTTTTGGGCAGGCTGGGACTGTAGTGCATGCTGAGGTAATTTATACTTGGACACAATACGAAATGTTTGGATGTGTAACCATGAGTACCGTTGAAGAAGTTGAGAAGGCTATGAAGAAGTTCAATGGCTATGAATTAGCTGGAAATTTCTTGACTGTTAGTAAGGATGATATAAGAAAAACACTACCTGATCTCCCAAAACGGCCACGCGCATTTGATTCTCCTTTTAGTGTCTATGTTACTCAGTTGCCACGGAGTGTTGAGAGTAATGAACACTTGGAGCAATTATTCAGCAAACATGGTAAGGTTGAGAGTGCTGAGTTAATCTGTAACAAAGTCTCAGGTCGATCACAGGGTTATGGATTTGTTACAATGTCTAGTGAGACTGATATGAAAAATGTCATTGCTGCTCTTCACGGTCAGGTAAATATTGGTAACATACATTTAAATCTGCTCAACTGA

Protein sequence

>Msa1258930

MLLSLSSSYKTLHHMIILQGFRPRSLSPPEGEEPAILLFSHLRLVMLEILLKIYVGNLTQDTDVEELKTLFGQAGTVVHAEVIYTWTQYEMFGCVTMSTVEEVEKAMKKFNGYELAGNFLTVSKDDIRKTLPDLPKRPRAFDSPFSVYVTQLPRSVESNEHLEQLFSKHGKVESAELICNKVSGRSQGYGFVTMSSETDMKNVIAALHGQVNIGNIHLNLLN*