Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1280140 | sp|Q58FX0|BAP2_ARATH | 34.634 | 205 | 100 | 6 | 1 | 176 | 6 | 205 | 4.86e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1280140 | A0A2Z6MN52 | 87.079 | 178 | 22 | 1 | 1 | 178 | 1 | 177 | 1.42e-111 | 324 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1238690 | Msa1280140 | 0.830108 | 3.536602e-55 | -8.615850e-47 |
| Msa1280140 | Msa1280150 | 0.805391 | 1.365254e-49 | -8.615850e-47 |
| Msa1280140 | Msa1323280 | 0.805475 | 1.311437e-49 | -8.615850e-47 |
| Msa1280140 | Msa1391550 | 0.800825 | 1.201744e-48 | -8.615850e-47 |
| Msa1184750 | Msa1280140 | 0.833975 | 3.915881e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1280140 | MtrunA17_Chr8g0350191 | 93.855 | 179 | 10 | 1 | 1 | 178 | 1 | 179 | 2.96e-113 | 319 |
| Msa1280140 | MtrunA17_Chr8g0350201 | 93.296 | 179 | 11 | 1 | 1 | 178 | 1 | 179 | 5.84e-113 | 318 |
| Msa1280140 | MtrunA17_Chr8g0350211 | 91.061 | 179 | 15 | 1 | 1 | 178 | 1 | 179 | 2.21e-108 | 307 |
| Msa1280140 | MtrunA17_Chr7g0246561 | 50.549 | 182 | 69 | 6 | 1 | 177 | 1 | 166 | 1.23e-51 | 163 |
| Msa1280140 | MtrunA17_Chr8g0350271 | 37.705 | 183 | 97 | 5 | 2 | 174 | 5 | 180 | 1.07e-28 | 105 |
| Msa1280140 | MtrunA17_Chr8g0350221 | 36.066 | 183 | 100 | 5 | 2 | 174 | 5 | 180 | 7.28e-28 | 102 |
| Msa1280140 | MtrunA17_Chr8g0350231 | 36.158 | 177 | 98 | 3 | 2 | 168 | 5 | 176 | 2.83e-27 | 101 |
| Msa1280140 | MtrunA17_Chr8g0350241 | 35.165 | 182 | 102 | 5 | 2 | 174 | 5 | 179 | 2.45e-24 | 94.0 |
| Msa1280140 | MtrunA17_Chr8g0350291 | 35.165 | 182 | 102 | 5 | 2 | 174 | 5 | 179 | 1.14e-23 | 92.0 |
| Msa1280140 | MtrunA17_Chr3g0109941 | 34.066 | 182 | 104 | 5 | 2 | 174 | 5 | 179 | 2.36e-22 | 88.6 |
| Msa1280140 | MtrunA17_Chr8g0350281 | 32.530 | 166 | 98 | 4 | 2 | 156 | 7 | 169 | 5.59e-21 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1280140 | AT2G45760.1 | 34.634 | 205 | 100 | 6 | 1 | 176 | 6 | 205 | 4.94e-32 | 114 |
| Msa1280140 | AT3G61190.1 | 36.066 | 183 | 106 | 6 | 1 | 178 | 16 | 192 | 5.22e-27 | 101 |
| Msa1280140 | AT3G61190.2 | 36.066 | 183 | 106 | 6 | 1 | 178 | 16 | 192 | 5.22e-27 | 101 |
Find 43 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAAGCCATAAA+AGG | 0.217867 | 8_3:+18205296 | Msa1280140:CDS |
| TTATGGCTTTGTTGTTCTCT+TGG | 0.294333 | 8_3:-18205292 | None:intergenic |
| CAGCAGGGATTCCTGCTGTT+TGG | 0.345360 | 8_3:+18205738 | Msa1280140:CDS |
| TACCCTCTCAAATGTGTCTT+TGG | 0.346126 | 8_3:-18205769 | None:intergenic |
| GGTGTTCCGGTGGCCGGTAA+TGG | 0.375336 | 8_3:+18205698 | Msa1280140:CDS |
| ATAATAGAGTTCGCAGAAAT+GGG | 0.379540 | 8_3:+18205585 | Msa1280140:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.403293 | 8_3:-18205640 | None:intergenic |
| GATAATAGAGTTCGCAGAAA+TGG | 0.407974 | 8_3:+18205584 | Msa1280140:CDS |
| ATTAGAACAAGAACTTTGTT+GGG | 0.411878 | 8_3:-18205639 | None:intergenic |
| GTGAATGGAGTTCCGGTAAC+TGG | 0.413671 | 8_3:+18205677 | Msa1280140:CDS |
| TCGATGTCATCGACGGTGAA+TGG | 0.414254 | 8_3:+18205662 | Msa1280140:CDS |
| TACATACCCTCCCAGAAAAT+CGG | 0.417592 | 8_3:-18205522 | None:intergenic |
| CGTAGGGTAACTTCCGCCTT+CGG | 0.421093 | 8_3:-18205384 | None:intergenic |
| GTTACGAACCTTGCATGTAA+TGG | 0.433682 | 8_3:-18205434 | None:intergenic |
| CCCTGCTGCAACTCTGCTGC+AGG | 0.437005 | 8_3:-18205723 | None:intergenic |
| TCCTGCAGCAGAGTTGCAGC+AGG | 0.439575 | 8_3:+18205722 | Msa1280140:CDS |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8_3:-18205711 | None:intergenic |
| AGTTCCGGTAACTGGTGTTC+CGG | 0.460170 | 8_3:+18205685 | Msa1280140:CDS |
| TGGTAGTTTAACCAAACAGC+AGG | 0.487555 | 8_3:-18205749 | None:intergenic |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8_3:-18205369 | None:intergenic |
| CCTCCCAGAAAATCGGAAAC+AGG | 0.493008 | 8_3:-18205515 | None:intergenic |
| TTCTAATTCGATGTCATCGA+CGG | 0.494293 | 8_3:+18205655 | Msa1280140:CDS |
| AGAACAACAAAGCCATAAAA+GGG | 0.500399 | 8_3:+18205297 | Msa1280140:CDS |
| GCCACCGGAACACCAGTTAC+CGG | 0.504701 | 8_3:-18205689 | None:intergenic |
| TAGTAGTAATAGTGTTGGTA+TGG | 0.506711 | 8_3:+18205484 | Msa1280140:CDS |
| GTAACTGGTGTTCCGGTGGC+CGG | 0.509562 | 8_3:+18205692 | Msa1280140:CDS |
| ATCGACGGTGAATGGAGTTC+CGG | 0.517982 | 8_3:+18205670 | Msa1280140:CDS |
| GGTTGTAAATATTTCAGTAA+AGG | 0.518683 | 8_3:+18205607 | Msa1280140:CDS |
| TGCAAGGTTCGTAACAATAG+AGG | 0.533455 | 8_3:+18205442 | Msa1280140:CDS |
| CCCTACGTGGAACGAGAAGT+TGG | 0.563010 | 8_3:+18205400 | Msa1280140:CDS |
| CCAACTTCTCGTTCCACGTA+GGG | 0.563124 | 8_3:-18205400 | None:intergenic |
| AGAGGTAGTAGTAATAGTGT+TGG | 0.570429 | 8_3:+18205479 | Msa1280140:CDS |
| TCCGGTAACTGGTGTTCCGG+TGG | 0.577500 | 8_3:+18205688 | Msa1280140:CDS |
| TTTGTCACGGTTCAATGCGA+CGG | 0.618008 | 8_3:+18205326 | Msa1280140:CDS |
| CCTGCAGCAGAGTTGCAGCA+GGG | 0.618156 | 8_3:+18205723 | Msa1280140:CDS |
| GAGGTGAAGTACAAAACAAG+AGG | 0.620322 | 8_3:+18205461 | Msa1280140:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.631770 | 8_3:+18205371 | Msa1280140:CDS |
| AAAAGGGAACACATTTGTCA+CGG | 0.633602 | 8_3:+18205313 | Msa1280140:CDS |
| TCGACGTGCCATTACATGCA+AGG | 0.639724 | 8_3:+18205426 | Msa1280140:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8_3:+18205368 | Msa1280140:CDS |
| TAATAGAGTTCGCAGAAATG+GGG | 0.668203 | 8_3:+18205586 | Msa1280140:CDS |
| AAGGCGGAAGTTACCCTACG+TGG | 0.698408 | 8_3:+18205387 | Msa1280140:CDS |
| ACCAACTTCTCGTTCCACGT+AGG | 0.720845 | 8_3:-18205401 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | + | chr8_3:18205555-18205574 | Msa1280140:CDS | 15.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | + | chr8_3:18205561-18205580 | Msa1280140:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | + | chr8_3:18205562-18205581 | Msa1280140:CDS | 20.0% |
| ! | GGTTGTAAATATTTCAGTAA+AGG | + | chr8_3:18205607-18205626 | Msa1280140:CDS | 25.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | - | chr8_3:18205642-18205661 | None:intergenic | 25.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | - | chr8_3:18205643-18205662 | None:intergenic | 25.0% |
| ! | AGAGATAACTATTTTGTCAG+CGG | + | chr8_3:18205262-18205281 | Msa1280140:CDS | 30.0% |
| AGAACAACAAAGCCATAAAA+GGG | + | chr8_3:18205297-18205316 | Msa1280140:CDS | 30.0% | |
| !! | TAGTAGTAATAGTGTTGGTA+TGG | + | chr8_3:18205484-18205503 | Msa1280140:CDS | 30.0% |
| ATAATAGAGTTCGCAGAAAT+GGG | + | chr8_3:18205585-18205604 | Msa1280140:CDS | 30.0% | |
| !! | TTATGGCTTTGTTGTTCTCT+TGG | - | chr8_3:18205295-18205314 | None:intergenic | 35.0% |
| GAGAACAACAAAGCCATAAA+AGG | + | chr8_3:18205296-18205315 | Msa1280140:CDS | 35.0% | |
| ! | GACAAATGTGTTCCCTTTTA+TGG | - | chr8_3:18205312-18205331 | None:intergenic | 35.0% |
| AAAAGGGAACACATTTGTCA+CGG | + | chr8_3:18205313-18205332 | Msa1280140:CDS | 35.0% | |
| AGAGGTAGTAGTAATAGTGT+TGG | + | chr8_3:18205479-18205498 | Msa1280140:CDS | 35.0% | |
| GATAATAGAGTTCGCAGAAA+TGG | + | chr8_3:18205584-18205603 | Msa1280140:CDS | 35.0% | |
| TAATAGAGTTCGCAGAAATG+GGG | + | chr8_3:18205586-18205605 | Msa1280140:CDS | 35.0% | |
| TTCTAATTCGATGTCATCGA+CGG | + | chr8_3:18205655-18205674 | Msa1280140:CDS | 35.0% | |
| GTTACGAACCTTGCATGTAA+TGG | - | chr8_3:18205437-18205456 | None:intergenic | 40.0% | |
| TGCAAGGTTCGTAACAATAG+AGG | + | chr8_3:18205442-18205461 | Msa1280140:CDS | 40.0% | |
| GAGGTGAAGTACAAAACAAG+AGG | + | chr8_3:18205461-18205480 | Msa1280140:CDS | 40.0% | |
| ! | AGTACCTGTTTCCGATTTTC+TGG | + | chr8_3:18205511-18205530 | Msa1280140:CDS | 40.0% |
| ! | GTACCTGTTTCCGATTTTCT+GGG | + | chr8_3:18205512-18205531 | Msa1280140:CDS | 40.0% |
| TACATACCCTCCCAGAAAAT+CGG | - | chr8_3:18205525-18205544 | None:intergenic | 40.0% | |
| TGGTAGTTTAACCAAACAGC+AGG | - | chr8_3:18205752-18205771 | None:intergenic | 40.0% | |
| TTTGTCACGGTTCAATGCGA+CGG | + | chr8_3:18205326-18205345 | Msa1280140:CDS | 45.0% | |
| ACCACGAAACTTGATTCCGA+AGG | + | chr8_3:18205368-18205387 | Msa1280140:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | + | chr8_3:18205371-18205390 | Msa1280140:CDS | 45.0% | |
| !! | CTGTTTCCGATTTTCTGGGA+GGG | + | chr8_3:18205516-18205535 | Msa1280140:CDS | 45.0% |
| GCCTTCGGAATCAAGTTTCG+TGG | - | chr8_3:18205372-18205391 | None:intergenic | 50.0% | |
| CCAACTTCTCGTTCCACGTA+GGG | - | chr8_3:18205403-18205422 | None:intergenic | 50.0% | |
| ACCAACTTCTCGTTCCACGT+AGG | - | chr8_3:18205404-18205423 | None:intergenic | 50.0% | |
| ! | TCGACGTGCCATTACATGCA+AGG | + | chr8_3:18205426-18205445 | Msa1280140:CDS | 50.0% |
| CCTCCCAGAAAATCGGAAAC+AGG | - | chr8_3:18205518-18205537 | None:intergenic | 50.0% | |
| ! | CCTGTTTCCGATTTTCTGGG+AGG | + | chr8_3:18205515-18205534 | Msa1280140:CDS | 50.0% |
| TCGATGTCATCGACGGTGAA+TGG | + | chr8_3:18205662-18205681 | Msa1280140:CDS | 50.0% | |
| ATCGACGGTGAATGGAGTTC+CGG | + | chr8_3:18205670-18205689 | Msa1280140:CDS | 50.0% | |
| GTGAATGGAGTTCCGGTAAC+TGG | + | chr8_3:18205677-18205696 | Msa1280140:CDS | 50.0% | |
| AGTTCCGGTAACTGGTGTTC+CGG | + | chr8_3:18205685-18205704 | Msa1280140:CDS | 50.0% | |
| CGTAGGGTAACTTCCGCCTT+CGG | - | chr8_3:18205387-18205406 | None:intergenic | 55.0% | |
| AAGGCGGAAGTTACCCTACG+TGG | + | chr8_3:18205387-18205406 | Msa1280140:CDS | 55.0% | |
| CCCTACGTGGAACGAGAAGT+TGG | + | chr8_3:18205400-18205419 | Msa1280140:CDS | 55.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | - | chr8_3:18205714-18205733 | None:intergenic | 55.0% | |
| CAGCAGGGATTCCTGCTGTT+TGG | + | chr8_3:18205738-18205757 | Msa1280140:CDS | 55.0% | |
| TCCGGTAACTGGTGTTCCGG+TGG | + | chr8_3:18205688-18205707 | Msa1280140:CDS | 60.0% | |
| GCCACCGGAACACCAGTTAC+CGG | - | chr8_3:18205692-18205711 | None:intergenic | 60.0% | |
| GTAACTGGTGTTCCGGTGGC+CGG | + | chr8_3:18205692-18205711 | Msa1280140:CDS | 60.0% | |
| TCCTGCAGCAGAGTTGCAGC+AGG | + | chr8_3:18205722-18205741 | Msa1280140:CDS | 60.0% | |
| CCTGCAGCAGAGTTGCAGCA+GGG | + | chr8_3:18205723-18205742 | Msa1280140:CDS | 60.0% | |
| GGTGTTCCGGTGGCCGGTAA+TGG | + | chr8_3:18205698-18205717 | Msa1280140:CDS | 65.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | - | chr8_3:18205707-18205726 | None:intergenic | 65.0% | |
| CCCTGCTGCAACTCTGCTGC+AGG | - | chr8_3:18205726-18205745 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 18205248 | 18205784 | 18205248 | ID=Msa1280140;Name=Msa1280140 |
| chr8_3 | mRNA | 18205248 | 18205784 | 18205248 | ID=Msa1280140-mRNA-1;Parent=Msa1280140;Name=Msa1280140-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|178 |
| chr8_3 | exon | 18205248 | 18205784 | 18205248 | ID=Msa1280140-mRNA-1:exon:6913;Parent=Msa1280140-mRNA-1 |
| chr8_3 | CDS | 18205248 | 18205784 | 18205248 | ID=Msa1280140-mRNA-1:cds;Parent=Msa1280140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
