Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323280 | sp|Q58FX0|BAP2_ARATH | 37.186 | 199 | 90 | 8 | 1 | 169 | 12 | 205 | 4.68e-30 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323280 | A0A2Z6MN52 | 87.209 | 172 | 20 | 2 | 1 | 171 | 7 | 177 | 1.22e-106 | 311 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0106530 | Msa1323280 | 0.814901 | 1.216504e-51 | -8.615850e-47 |
| Msa0775920 | Msa1323280 | 0.806657 | 7.394971e-50 | -8.615850e-47 |
| Msa1280140 | Msa1323280 | 0.805475 | 1.311437e-49 | -8.615850e-47 |
| Msa1323280 | Msa1363810 | 0.848593 | 5.554114e-60 | -8.615850e-47 |
| Msa1323280 | Msa1391550 | 0.801432 | 9.027662e-49 | -8.615850e-47 |
| Msa1323280 | Msa1405820 | 0.813759 | 2.175587e-51 | -8.615850e-47 |
| Msa0437070 | Msa1323280 | 0.812676 | 3.761490e-51 | -8.615850e-47 |
| Msa0259440 | Msa1323280 | 0.801862 | 7.370427e-49 | -8.615850e-47 |
| Msa1123720 | Msa1323280 | 0.808603 | 2.855856e-50 | -8.615850e-47 |
| Msa1184750 | Msa1323280 | 0.922686 | 7.119165e-89 | -8.615850e-47 |
| Msa1184760 | Msa1323280 | 0.854286 | 1.362030e-61 | -8.615850e-47 |
| Msa0572560 | Msa1323280 | 0.852266 | 5.168303e-61 | -8.615850e-47 |
| Msa0853810 | Msa1323280 | 0.805534 | 1.274240e-49 | -8.615850e-47 |
| Msa0968050 | Msa1323280 | 0.802288 | 6.024067e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323280 | MtrunA17_Chr8g0350201 | 87.861 | 173 | 19 | 2 | 1 | 171 | 7 | 179 | 7.33e-106 | 300 |
| Msa1323280 | MtrunA17_Chr8g0350191 | 87.283 | 173 | 20 | 2 | 1 | 171 | 7 | 179 | 7.33e-106 | 300 |
| Msa1323280 | MtrunA17_Chr8g0350211 | 89.017 | 173 | 17 | 2 | 1 | 171 | 7 | 179 | 1.20e-104 | 297 |
| Msa1323280 | MtrunA17_Chr7g0246561 | 53.143 | 175 | 62 | 6 | 1 | 170 | 7 | 166 | 1.98e-51 | 162 |
| Msa1323280 | MtrunA17_Chr8g0350271 | 36.517 | 178 | 95 | 5 | 1 | 167 | 10 | 180 | 1.93e-25 | 96.3 |
| Msa1323280 | MtrunA17_Chr8g0350221 | 35.393 | 178 | 97 | 5 | 1 | 167 | 10 | 180 | 2.83e-25 | 95.9 |
| Msa1323280 | MtrunA17_Chr8g0350231 | 34.884 | 172 | 96 | 3 | 1 | 161 | 10 | 176 | 3.96e-24 | 92.8 |
| Msa1323280 | MtrunA17_Chr8g0350241 | 33.898 | 177 | 100 | 5 | 1 | 167 | 10 | 179 | 5.47e-21 | 84.7 |
| Msa1323280 | MtrunA17_Chr8g0350291 | 34.463 | 177 | 99 | 5 | 1 | 167 | 10 | 179 | 1.16e-20 | 84.0 |
| Msa1323280 | MtrunA17_Chr3g0109941 | 32.768 | 177 | 102 | 5 | 1 | 167 | 10 | 179 | 4.83e-20 | 82.4 |
| Msa1323280 | MtrunA17_Chr8g0350281 | 32.500 | 160 | 95 | 3 | 1 | 149 | 12 | 169 | 1.18e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1323280 | AT2G45760.1 | 37.186 | 199 | 90 | 8 | 1 | 169 | 12 | 205 | 4.76e-31 | 112 |
| Msa1323280 | AT3G61190.1 | 37.500 | 176 | 100 | 6 | 1 | 171 | 22 | 192 | 4.08e-26 | 98.6 |
| Msa1323280 | AT3G61190.2 | 37.500 | 176 | 100 | 6 | 1 | 171 | 22 | 192 | 4.08e-26 | 98.6 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAAGCCATAAA+AGG | 0.217867 | 8_4:+31522633 | Msa1323280:CDS |
| CACCCTTTCAAATGTGTCTT+TGG | 0.267837 | 8_4:-31523103 | None:intergenic |
| TTATGGCTTTGTTGTTCTCT+TGG | 0.294333 | 8_4:-31522629 | None:intergenic |
| CAACAGGGATTCCTGCTGTT+TGG | 0.341709 | 8_4:+31523072 | Msa1323280:CDS |
| ATAATAGAGTTAGGAGAAAC+GGG | 0.341844 | 8_4:+31522922 | Msa1323280:CDS |
| GGTGTTCCGGTGGCCGGTAA+TGG | 0.375336 | 8_4:+31523032 | Msa1323280:CDS |
| TCCTGCAGCAGAGTTGCAAC+AGG | 0.395408 | 8_4:+31523056 | Msa1323280:CDS |
| ATTAGTGGAGTTCCGGTAAC+TGG | 0.400709 | 8_4:+31523011 | Msa1323280:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.403293 | 8_4:-31522977 | None:intergenic |
| ATTAGAACAAGAACTTTGTT+GGG | 0.411878 | 8_4:-31522976 | None:intergenic |
| GTCATCGATTAGTGGAGTTC+CGG | 0.435279 | 8_4:+31523004 | Msa1323280:CDS |
| GACATACCCTCCAAGAAAAT+CGG | 0.435714 | 8_4:-31522859 | None:intergenic |
| CCCTGTTGCAACTCTGCTGC+AGG | 0.438745 | 8_4:-31523057 | None:intergenic |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8_4:-31523045 | None:intergenic |
| AGTTCCGGTAACTGGTGTTC+CGG | 0.450510 | 8_4:+31523019 | Msa1323280:CDS |
| AATTCGATGTCATCGATTAG+TGG | 0.458468 | 8_4:+31522996 | Msa1323280:CDS |
| AGTAGGGTAACTTCCGCCTT+CGG | 0.464742 | 8_4:-31522721 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8_4:+31522913 | Msa1323280:CDS |
| CCAACTTCTCGTTCCAAGTA+GGG | 0.480749 | 8_4:-31522737 | None:intergenic |
| AAGTAGTAGCAGTGTTGGTA+TGG | 0.480912 | 8_4:+31522821 | Msa1323280:CDS |
| AAGGCGGAAGTTACCCTACT+TGG | 0.483567 | 8_4:+31522724 | Msa1323280:CDS |
| TGGTAGTTTAACCAAACAGC+AGG | 0.487555 | 8_4:-31523083 | None:intergenic |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8_4:-31522706 | None:intergenic |
| GTTATGAACCTTGCATGTAA+TGG | 0.492338 | 8_4:-31522771 | None:intergenic |
| AGAACAACAAAGCCATAAAA+GGG | 0.500399 | 8_4:+31522634 | Msa1323280:CDS |
| GCCACCGGAACACCAGTTAC+CGG | 0.504701 | 8_4:-31523023 | None:intergenic |
| GATAATAGAGTTAGGAGAAA+CGG | 0.505051 | 8_4:+31522921 | Msa1323280:CDS |
| GTAACTGGTGTTCCGGTGGC+CGG | 0.509562 | 8_4:+31523026 | Msa1323280:CDS |
| TGTTGGTATGGCTAGAATAC+CGG | 0.512707 | 8_4:+31522833 | Msa1323280:CDS |
| AGAGGAAGTAGTAGCAGTGT+TGG | 0.515573 | 8_4:+31522816 | Msa1323280:CDS |
| CCTCCAAGAAAATCGGAAAC+CGG | 0.520232 | 8_4:-31522852 | None:intergenic |
| TGCAAGGTTCATAACAATAG+AGG | 0.521084 | 8_4:+31522779 | Msa1323280:CDS |
| TTGGAACGAGAAGTTGGTAA+TGG | 0.534411 | 8_4:+31522743 | Msa1323280:CDS |
| CCCTACTTGGAACGAGAAGT+TGG | 0.543637 | 8_4:+31522737 | Msa1323280:CDS |
| TCCGGTAACTGGTGTTCCGG+TGG | 0.577500 | 8_4:+31523022 | Msa1323280:CDS |
| GGTTGTAAATATTTCAGTGA+AGG | 0.577908 | 8_4:+31522944 | Msa1323280:CDS |
| ACCAACTTCTCGTTCCAAGT+AGG | 0.585914 | 8_4:-31522738 | None:intergenic |
| TTTGTCACGGTTCAATGTGA+CGG | 0.605787 | 8_4:+31522663 | Msa1323280:CDS |
| TGGATGTGCCATTACATGCA+AGG | 0.624126 | 8_4:+31522763 | Msa1323280:CDS |
| GAGGTGAAGTACAAAACTAG+AGG | 0.629180 | 8_4:+31522798 | Msa1323280:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.631770 | 8_4:+31522708 | Msa1323280:CDS |
| AAAAGGGAACACATTTGTCA+CGG | 0.633602 | 8_4:+31522650 | Msa1323280:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8_4:+31522705 | Msa1323280:CDS |
| CCTGCAGCAGAGTTGCAACA+GGG | 0.674047 | 8_4:+31523057 | Msa1323280:CDS |
| TAATAGAGTTAGGAGAAACG+GGG | 0.700815 | 8_4:+31522923 | Msa1323280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | + | chr8_4:31522892-31522911 | Msa1323280:CDS | 15.0% |
| !!! | AAAATTGCAATTGATTTTCA+TGG | - | chr8_4:31522884-31522903 | None:intergenic | 20.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | + | chr8_4:31522898-31522917 | Msa1323280:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | + | chr8_4:31522899-31522918 | Msa1323280:CDS | 20.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | - | chr8_4:31522979-31522998 | None:intergenic | 25.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | - | chr8_4:31522980-31522999 | None:intergenic | 25.0% |
| AGAACAACAAAGCCATAAAA+GGG | + | chr8_4:31522634-31522653 | Msa1323280:CDS | 30.0% | |
| GATAATAGAGTTAGGAGAAA+CGG | + | chr8_4:31522921-31522940 | Msa1323280:CDS | 30.0% | |
| ATAATAGAGTTAGGAGAAAC+GGG | + | chr8_4:31522922-31522941 | Msa1323280:CDS | 30.0% | |
| GGTTGTAAATATTTCAGTGA+AGG | + | chr8_4:31522944-31522963 | Msa1323280:CDS | 30.0% | |
| !! | TTATGGCTTTGTTGTTCTCT+TGG | - | chr8_4:31522632-31522651 | None:intergenic | 35.0% |
| GAGAACAACAAAGCCATAAA+AGG | + | chr8_4:31522633-31522652 | Msa1323280:CDS | 35.0% | |
| ! | GACAAATGTGTTCCCTTTTA+TGG | - | chr8_4:31522649-31522668 | None:intergenic | 35.0% |
| AAAAGGGAACACATTTGTCA+CGG | + | chr8_4:31522650-31522669 | Msa1323280:CDS | 35.0% | |
| GTTATGAACCTTGCATGTAA+TGG | - | chr8_4:31522774-31522793 | None:intergenic | 35.0% | |
| TGCAAGGTTCATAACAATAG+AGG | + | chr8_4:31522779-31522798 | Msa1323280:CDS | 35.0% | |
| TAATAGAGTTAGGAGAAACG+GGG | + | chr8_4:31522923-31522942 | Msa1323280:CDS | 35.0% | |
| ! | AATTCGATGTCATCGATTAG+TGG | + | chr8_4:31522996-31523015 | Msa1323280:CDS | 35.0% |
| TTTGTCACGGTTCAATGTGA+CGG | + | chr8_4:31522663-31522682 | Msa1323280:CDS | 40.0% | |
| !! | TTGGAACGAGAAGTTGGTAA+TGG | + | chr8_4:31522743-31522762 | Msa1323280:CDS | 40.0% |
| GAGGTGAAGTACAAAACTAG+AGG | + | chr8_4:31522798-31522817 | Msa1323280:CDS | 40.0% | |
| !! | AAGTAGTAGCAGTGTTGGTA+TGG | + | chr8_4:31522821-31522840 | Msa1323280:CDS | 40.0% |
| TGTTGGTATGGCTAGAATAC+CGG | + | chr8_4:31522833-31522852 | Msa1323280:CDS | 40.0% | |
| !!! | ATACCGGTTTCCGATTTTCT+TGG | + | chr8_4:31522849-31522868 | Msa1323280:CDS | 40.0% |
| GACATACCCTCCAAGAAAAT+CGG | - | chr8_4:31522862-31522881 | None:intergenic | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | + | chr8_4:31522913-31522932 | Msa1323280:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+AGG | - | chr8_4:31523086-31523105 | None:intergenic | 40.0% | |
| ACCACGAAACTTGATTCCGA+AGG | + | chr8_4:31522705-31522724 | Msa1323280:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | + | chr8_4:31522708-31522727 | Msa1323280:CDS | 45.0% | |
| CCAACTTCTCGTTCCAAGTA+GGG | - | chr8_4:31522740-31522759 | None:intergenic | 45.0% | |
| ACCAACTTCTCGTTCCAAGT+AGG | - | chr8_4:31522741-31522760 | None:intergenic | 45.0% | |
| ! | TGGATGTGCCATTACATGCA+AGG | + | chr8_4:31522763-31522782 | Msa1323280:CDS | 45.0% |
| AGAGGAAGTAGTAGCAGTGT+TGG | + | chr8_4:31522816-31522835 | Msa1323280:CDS | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+CGG | - | chr8_4:31522855-31522874 | None:intergenic | 45.0% | |
| ! | CGGTTTCCGATTTTCTTGGA+GGG | + | chr8_4:31522853-31522872 | Msa1323280:CDS | 45.0% |
| ! | GTCATCGATTAGTGGAGTTC+CGG | + | chr8_4:31523004-31523023 | Msa1323280:CDS | 45.0% |
| ATTAGTGGAGTTCCGGTAAC+TGG | + | chr8_4:31523011-31523030 | Msa1323280:CDS | 45.0% | |
| GCCTTCGGAATCAAGTTTCG+TGG | - | chr8_4:31522709-31522728 | None:intergenic | 50.0% | |
| AGTAGGGTAACTTCCGCCTT+CGG | - | chr8_4:31522724-31522743 | None:intergenic | 50.0% | |
| AAGGCGGAAGTTACCCTACT+TGG | + | chr8_4:31522724-31522743 | Msa1323280:CDS | 50.0% | |
| CCCTACTTGGAACGAGAAGT+TGG | + | chr8_4:31522737-31522756 | Msa1323280:CDS | 50.0% | |
| ! | CCGGTTTCCGATTTTCTTGG+AGG | + | chr8_4:31522852-31522871 | Msa1323280:CDS | 50.0% |
| AGTTCCGGTAACTGGTGTTC+CGG | + | chr8_4:31523019-31523038 | Msa1323280:CDS | 50.0% | |
| CAACAGGGATTCCTGCTGTT+TGG | + | chr8_4:31523072-31523091 | Msa1323280:CDS | 50.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | - | chr8_4:31523048-31523067 | None:intergenic | 55.0% | |
| TCCTGCAGCAGAGTTGCAAC+AGG | + | chr8_4:31523056-31523075 | Msa1323280:CDS | 55.0% | |
| CCTGCAGCAGAGTTGCAACA+GGG | + | chr8_4:31523057-31523076 | Msa1323280:CDS | 55.0% | |
| TCCGGTAACTGGTGTTCCGG+TGG | + | chr8_4:31523022-31523041 | Msa1323280:CDS | 60.0% | |
| GCCACCGGAACACCAGTTAC+CGG | - | chr8_4:31523026-31523045 | None:intergenic | 60.0% | |
| GTAACTGGTGTTCCGGTGGC+CGG | + | chr8_4:31523026-31523045 | Msa1323280:CDS | 60.0% | |
| CCCTGTTGCAACTCTGCTGC+AGG | - | chr8_4:31523060-31523079 | None:intergenic | 60.0% | |
| GGTGTTCCGGTGGCCGGTAA+TGG | + | chr8_4:31523032-31523051 | Msa1323280:CDS | 65.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | - | chr8_4:31523041-31523060 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 31522603 | 31523118 | 31522603 | ID=Msa1323280;Name=Msa1323280 |
| chr8_4 | mRNA | 31522603 | 31523118 | 31522603 | ID=Msa1323280-mRNA-1;Parent=Msa1323280;Name=Msa1323280-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|171 |
| chr8_4 | exon | 31522603 | 31523118 | 31522603 | ID=Msa1323280-mRNA-1:exon:10249;Parent=Msa1323280-mRNA-1 |
| chr8_4 | CDS | 31522603 | 31523118 | 31522603 | ID=Msa1323280-mRNA-1:cds;Parent=Msa1323280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
