Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1184750 | sp|Q58FX0|BAP2_ARATH | 36.585 | 205 | 96 | 6 | 1 | 176 | 6 | 205 | 9.63e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1184750 | A0A2Z6MN52 | 87.640 | 178 | 21 | 1 | 1 | 178 | 1 | 177 | 3.22e-112 | 326 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0106530 | Msa1184750 | 0.813202 | 2.883822e-51 | -8.615850e-47 |
| Msa0437070 | Msa1184750 | 0.807235 | 5.581882e-50 | -8.615850e-47 |
| Msa0259440 | Msa1184750 | 0.825811 | 3.823467e-54 | -8.615850e-47 |
| Msa0259580 | Msa1184750 | 0.820408 | 6.968935e-53 | -8.615850e-47 |
| Msa0305620 | Msa1184750 | 0.813250 | 2.815601e-51 | -8.615850e-47 |
| Msa1109670 | Msa1184750 | 0.802842 | 4.630867e-49 | -8.615850e-47 |
| Msa1184750 | Msa1184760 | 0.851872 | 6.688034e-61 | -8.615850e-47 |
| Msa1184750 | Msa1238690 | 0.818676 | 1.731197e-52 | -8.615850e-47 |
| Msa1184750 | Msa1280140 | 0.833975 | 3.915881e-56 | -8.615850e-47 |
| Msa1184750 | Msa1323280 | 0.922686 | 7.119165e-89 | -8.615850e-47 |
| Msa1184750 | Msa1363810 | 0.836710 | 7.979461e-57 | -8.615850e-47 |
| Msa1184750 | Msa1376450 | 0.811555 | 6.602378e-51 | -8.615850e-47 |
| Msa1184750 | Msa1391550 | 0.815658 | 8.260379e-52 | -8.615850e-47 |
| Msa1184750 | Msa1405820 | 0.805566 | 1.254673e-49 | -8.615850e-47 |
| Msa0572560 | Msa1184750 | 0.845982 | 2.891287e-59 | -8.615850e-47 |
| Msa0853810 | Msa1184750 | 0.808034 | 3.775673e-50 | -8.615850e-47 |
| Msa0968050 | Msa1184750 | 0.828616 | 8.141684e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1184750 | MtrunA17_Chr8g0350191 | 91.620 | 179 | 14 | 1 | 1 | 178 | 1 | 179 | 3.13e-112 | 317 |
| Msa1184750 | MtrunA17_Chr8g0350201 | 92.179 | 179 | 13 | 1 | 1 | 178 | 1 | 179 | 3.53e-112 | 317 |
| Msa1184750 | MtrunA17_Chr8g0350211 | 91.620 | 179 | 14 | 1 | 1 | 178 | 1 | 179 | 8.32e-110 | 310 |
| Msa1184750 | MtrunA17_Chr7g0246561 | 53.039 | 181 | 64 | 6 | 1 | 176 | 1 | 165 | 8.50e-54 | 168 |
| Msa1184750 | MtrunA17_Chr8g0350271 | 37.705 | 183 | 97 | 5 | 2 | 174 | 5 | 180 | 2.98e-28 | 103 |
| Msa1184750 | MtrunA17_Chr8g0350221 | 36.612 | 183 | 99 | 5 | 2 | 174 | 5 | 180 | 5.16e-28 | 103 |
| Msa1184750 | MtrunA17_Chr8g0350231 | 36.158 | 177 | 98 | 3 | 2 | 168 | 5 | 176 | 7.45e-27 | 100 |
| Msa1184750 | MtrunA17_Chr8g0350241 | 35.714 | 182 | 101 | 5 | 2 | 174 | 5 | 179 | 1.39e-24 | 94.4 |
| Msa1184750 | MtrunA17_Chr8g0350291 | 34.615 | 182 | 103 | 5 | 2 | 174 | 5 | 179 | 4.88e-23 | 90.5 |
| Msa1184750 | MtrunA17_Chr3g0109941 | 33.516 | 182 | 105 | 5 | 2 | 174 | 5 | 179 | 2.31e-22 | 88.6 |
| Msa1184750 | MtrunA17_Chr8g0350281 | 31.928 | 166 | 99 | 4 | 2 | 156 | 7 | 169 | 1.52e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1184750 | AT2G45760.1 | 36.585 | 205 | 96 | 6 | 1 | 176 | 6 | 205 | 9.79e-34 | 119 |
| Msa1184750 | AT3G61190.1 | 37.705 | 183 | 103 | 6 | 1 | 178 | 16 | 192 | 1.60e-28 | 105 |
| Msa1184750 | AT3G61190.2 | 37.705 | 183 | 103 | 6 | 1 | 178 | 16 | 192 | 1.60e-28 | 105 |
Find 44 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAAGCCATAAA+AGG | 0.217867 | 8_1:+20872533 | Msa1184750:CDS |
| TACACTTTCAAATGTGTCTT+TGG | 0.278737 | 8_1:-20873006 | None:intergenic |
| TTATGGCTTTGTTGTTCTCT+TGG | 0.294333 | 8_1:-20872529 | None:intergenic |
| ATAATAGAGTTAGGAGAAAT+GGG | 0.321912 | 8_1:+20872822 | Msa1184750:CDS |
| GATAATAGAGTTAGGAGAAA+TGG | 0.337385 | 8_1:+20872821 | Msa1184750:CDS |
| CAACAGGGATTCCTGCTGTT+TGG | 0.341709 | 8_1:+20872975 | Msa1184750:CDS |
| GGCGTTCCGGTGGCCGGTAA+TGG | 0.366500 | 8_1:+20872935 | Msa1184750:CDS |
| GTGAATGGAGTTCCGGTAAC+TGG | 0.395156 | 8_1:+20872914 | Msa1184750:CDS |
| TCCTGCAGCAGAGTTGCAAC+AGG | 0.395408 | 8_1:+20872959 | Msa1184750:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.400854 | 8_1:-20872877 | None:intergenic |
| CACATACCCTCCAAGAAAAT+CGG | 0.422233 | 8_1:-20872759 | None:intergenic |
| ATTAGAACAAGAACTTTGTT+GGG | 0.425451 | 8_1:-20872876 | None:intergenic |
| CCCTGTTGCAACTCTGCTGC+AGG | 0.438745 | 8_1:-20872960 | None:intergenic |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8_1:-20872948 | None:intergenic |
| GCCACCGGAACGCCAGTTAC+CGG | 0.450510 | 8_1:-20872926 | None:intergenic |
| AGTTCCGGTAACTGGCGTTC+CGG | 0.456993 | 8_1:+20872922 | Msa1184750:CDS |
| AGTAGGGTAACTTCCGCCTT+CGG | 0.464742 | 8_1:-20872621 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8_1:+20872813 | Msa1184750:CDS |
| CCAACTTCTCGTTCCAAGTA+GGG | 0.480749 | 8_1:-20872637 | None:intergenic |
| AAGTAGTAGCAGTGTTGGTA+TGG | 0.480912 | 8_1:+20872721 | Msa1184750:CDS |
| AAGGCGGAAGTTACCCTACT+TGG | 0.483567 | 8_1:+20872624 | Msa1184750:CDS |
| TGGTAGTTTAACCAAACAGC+AGG | 0.487555 | 8_1:-20872986 | None:intergenic |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8_1:-20872606 | None:intergenic |
| TCAATGTCATCGTCGGTGAA+TGG | 0.489891 | 8_1:+20872899 | Msa1184750:CDS |
| GTTATGAACCTTGCATGTAA+TGG | 0.492338 | 8_1:-20872671 | None:intergenic |
| AGAACAACAAAGCCATAAAA+GGG | 0.500399 | 8_1:+20872534 | Msa1184750:CDS |
| TGTTGGTATGGCTAGAATAC+CGG | 0.512707 | 8_1:+20872733 | Msa1184750:CDS |
| AGAGGAAGTAGTAGCAGTGT+TGG | 0.515573 | 8_1:+20872716 | Msa1184750:CDS |
| ATCGTCGGTGAATGGAGTTC+CGG | 0.515963 | 8_1:+20872907 | Msa1184750:CDS |
| CCTCCAAGAAAATCGGAAAC+CGG | 0.520232 | 8_1:-20872752 | None:intergenic |
| TGCAAGGTTCATAACAATAG+AGG | 0.521084 | 8_1:+20872679 | Msa1184750:CDS |
| TTCTAATTCAATGTCATCGT+CGG | 0.527140 | 8_1:+20872892 | Msa1184750:CDS |
| TTGGAACGAGAAGTTGGTAA+TGG | 0.534411 | 8_1:+20872643 | Msa1184750:CDS |
| CCCTACTTGGAACGAGAAGT+TGG | 0.543637 | 8_1:+20872637 | Msa1184750:CDS |
| ACCAACTTCTCGTTCCAAGT+AGG | 0.585914 | 8_1:-20872638 | None:intergenic |
| TGGGGTTGTAAATATTTCAG+TGG | 0.590455 | 8_1:+20872841 | Msa1184750:CDS |
| TTTGTCACGGTTCAATGTGA+CGG | 0.605787 | 8_1:+20872563 | Msa1184750:CDS |
| TGGATGTGCCATTACATGCA+AGG | 0.624126 | 8_1:+20872663 | Msa1184750:CDS |
| GAGGTGAAGTACAAAACTAG+AGG | 0.629180 | 8_1:+20872698 | Msa1184750:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.631770 | 8_1:+20872608 | Msa1184750:CDS |
| AAAAGGGAACACATTTGTCA+CGG | 0.633602 | 8_1:+20872550 | Msa1184750:CDS |
| TAATAGAGTTAGGAGAAATG+GGG | 0.635926 | 8_1:+20872823 | Msa1184750:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8_1:+20872605 | Msa1184750:CDS |
| CCTGCAGCAGAGTTGCAACA+GGG | 0.674047 | 8_1:+20872960 | Msa1184750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | + | chr8_1:20872792-20872811 | Msa1184750:CDS | 15.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | + | chr8_1:20872798-20872817 | Msa1184750:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | + | chr8_1:20872799-20872818 | Msa1184750:CDS | 20.0% |
| ! | ATAATAGAGTTAGGAGAAAT+GGG | + | chr8_1:20872822-20872841 | Msa1184750:CDS | 25.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | - | chr8_1:20872879-20872898 | None:intergenic | 25.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | - | chr8_1:20872880-20872899 | None:intergenic | 25.0% |
| AGAACAACAAAGCCATAAAA+GGG | + | chr8_1:20872534-20872553 | Msa1184750:CDS | 30.0% | |
| GATAATAGAGTTAGGAGAAA+TGG | + | chr8_1:20872821-20872840 | Msa1184750:CDS | 30.0% | |
| TAATAGAGTTAGGAGAAATG+GGG | + | chr8_1:20872823-20872842 | Msa1184750:CDS | 30.0% | |
| TTCTAATTCAATGTCATCGT+CGG | + | chr8_1:20872892-20872911 | Msa1184750:CDS | 30.0% | |
| ! | AGAGATGACTATTTTGTCAG+CGG | + | chr8_1:20872499-20872518 | Msa1184750:CDS | 35.0% |
| !! | TTATGGCTTTGTTGTTCTCT+TGG | - | chr8_1:20872532-20872551 | None:intergenic | 35.0% |
| GAGAACAACAAAGCCATAAA+AGG | + | chr8_1:20872533-20872552 | Msa1184750:CDS | 35.0% | |
| ! | GACAAATGTGTTCCCTTTTA+TGG | - | chr8_1:20872549-20872568 | None:intergenic | 35.0% |
| AAAAGGGAACACATTTGTCA+CGG | + | chr8_1:20872550-20872569 | Msa1184750:CDS | 35.0% | |
| GTTATGAACCTTGCATGTAA+TGG | - | chr8_1:20872674-20872693 | None:intergenic | 35.0% | |
| TGCAAGGTTCATAACAATAG+AGG | + | chr8_1:20872679-20872698 | Msa1184750:CDS | 35.0% | |
| TGGGGTTGTAAATATTTCAG+TGG | + | chr8_1:20872841-20872860 | Msa1184750:CDS | 35.0% | |
| TTTGTCACGGTTCAATGTGA+CGG | + | chr8_1:20872563-20872582 | Msa1184750:CDS | 40.0% | |
| !! | TTGGAACGAGAAGTTGGTAA+TGG | + | chr8_1:20872643-20872662 | Msa1184750:CDS | 40.0% |
| GAGGTGAAGTACAAAACTAG+AGG | + | chr8_1:20872698-20872717 | Msa1184750:CDS | 40.0% | |
| !! | AAGTAGTAGCAGTGTTGGTA+TGG | + | chr8_1:20872721-20872740 | Msa1184750:CDS | 40.0% |
| TGTTGGTATGGCTAGAATAC+CGG | + | chr8_1:20872733-20872752 | Msa1184750:CDS | 40.0% | |
| !!! | ATACCGGTTTCCGATTTTCT+TGG | + | chr8_1:20872749-20872768 | Msa1184750:CDS | 40.0% |
| CACATACCCTCCAAGAAAAT+CGG | - | chr8_1:20872762-20872781 | None:intergenic | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | + | chr8_1:20872813-20872832 | Msa1184750:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+AGG | - | chr8_1:20872989-20873008 | None:intergenic | 40.0% | |
| ACCACGAAACTTGATTCCGA+AGG | + | chr8_1:20872605-20872624 | Msa1184750:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | + | chr8_1:20872608-20872627 | Msa1184750:CDS | 45.0% | |
| CCAACTTCTCGTTCCAAGTA+GGG | - | chr8_1:20872640-20872659 | None:intergenic | 45.0% | |
| ACCAACTTCTCGTTCCAAGT+AGG | - | chr8_1:20872641-20872660 | None:intergenic | 45.0% | |
| ! | TGGATGTGCCATTACATGCA+AGG | + | chr8_1:20872663-20872682 | Msa1184750:CDS | 45.0% |
| AGAGGAAGTAGTAGCAGTGT+TGG | + | chr8_1:20872716-20872735 | Msa1184750:CDS | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+CGG | - | chr8_1:20872755-20872774 | None:intergenic | 45.0% | |
| ! | CGGTTTCCGATTTTCTTGGA+GGG | + | chr8_1:20872753-20872772 | Msa1184750:CDS | 45.0% |
| TCAATGTCATCGTCGGTGAA+TGG | + | chr8_1:20872899-20872918 | Msa1184750:CDS | 45.0% | |
| GCCTTCGGAATCAAGTTTCG+TGG | - | chr8_1:20872609-20872628 | None:intergenic | 50.0% | |
| AGTAGGGTAACTTCCGCCTT+CGG | - | chr8_1:20872624-20872643 | None:intergenic | 50.0% | |
| AAGGCGGAAGTTACCCTACT+TGG | + | chr8_1:20872624-20872643 | Msa1184750:CDS | 50.0% | |
| CCCTACTTGGAACGAGAAGT+TGG | + | chr8_1:20872637-20872656 | Msa1184750:CDS | 50.0% | |
| ! | CCGGTTTCCGATTTTCTTGG+AGG | + | chr8_1:20872752-20872771 | Msa1184750:CDS | 50.0% |
| ATCGTCGGTGAATGGAGTTC+CGG | + | chr8_1:20872907-20872926 | Msa1184750:CDS | 50.0% | |
| GTGAATGGAGTTCCGGTAAC+TGG | + | chr8_1:20872914-20872933 | Msa1184750:CDS | 50.0% | |
| CAACAGGGATTCCTGCTGTT+TGG | + | chr8_1:20872975-20872994 | Msa1184750:CDS | 50.0% | |
| AGTTCCGGTAACTGGCGTTC+CGG | + | chr8_1:20872922-20872941 | Msa1184750:CDS | 55.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | - | chr8_1:20872951-20872970 | None:intergenic | 55.0% | |
| TCCTGCAGCAGAGTTGCAAC+AGG | + | chr8_1:20872959-20872978 | Msa1184750:CDS | 55.0% | |
| CCTGCAGCAGAGTTGCAACA+GGG | + | chr8_1:20872960-20872979 | Msa1184750:CDS | 55.0% | |
| CCCTGTTGCAACTCTGCTGC+AGG | - | chr8_1:20872963-20872982 | None:intergenic | 60.0% | |
| TCCGGTAACTGGCGTTCCGG+TGG | + | chr8_1:20872925-20872944 | Msa1184750:CDS | 65.0% | |
| GCCACCGGAACGCCAGTTAC+CGG | - | chr8_1:20872929-20872948 | None:intergenic | 65.0% | |
| GTAACTGGCGTTCCGGTGGC+CGG | + | chr8_1:20872929-20872948 | Msa1184750:CDS | 65.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | - | chr8_1:20872944-20872963 | None:intergenic | 65.0% | |
| GGCGTTCCGGTGGCCGGTAA+TGG | + | chr8_1:20872935-20872954 | Msa1184750:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 20872485 | 20873021 | 20872485 | ID=Msa1184750;Name=Msa1184750 |
| chr8_1 | mRNA | 20872485 | 20873021 | 20872485 | ID=Msa1184750-mRNA-1;Parent=Msa1184750;Name=Msa1184750-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|178 |
| chr8_1 | exon | 20872485 | 20873021 | 20872485 | ID=Msa1184750-mRNA-1:exon:7938;Parent=Msa1184750-mRNA-1 |
| chr8_1 | CDS | 20872485 | 20873021 | 20872485 | ID=Msa1184750-mRNA-1:cds;Parent=Msa1184750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
