AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1280150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1280150 MtrunA17_Chr8g0350211 96.648 179 6 0 1 179 1 179 3.41e-128 357
Msa1280150 MtrunA17_Chr8g0350191 90.503 179 17 0 1 179 1 179 6.73e-109 308
Msa1280150 MtrunA17_Chr8g0350201 89.944 179 18 0 1 179 1 179 2.57e-108 306
Msa1280150 MtrunA17_Chr7g0246561 54.396 182 63 5 1 178 1 166 1.63e-58 180
Msa1280150 MtrunA17_Chr8g0350271 38.043 184 96 6 2 175 5 180 7.21e-29 105
Msa1280150 MtrunA17_Chr8g0350231 36.517 178 97 4 2 169 5 176 2.38e-27 101
Msa1280150 MtrunA17_Chr8g0350221 35.870 184 100 6 2 175 5 180 4.71e-27 100
Msa1280150 MtrunA17_Chr8g0350241 36.066 183 100 6 2 175 5 179 2.64e-25 96.3
Msa1280150 MtrunA17_Chr8g0350291 34.973 183 102 6 2 175 5 179 5.40e-24 92.8
Msa1280150 MtrunA17_Chr3g0109941 34.973 183 102 7 2 175 5 179 7.95e-23 89.7
Msa1280150 MtrunA17_Chr8g0350281 32.749 171 105 5 2 171 7 168 4.65e-21 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1280150 AT2G45760.1 35.294 204 101 5 1 177 6 205 4.58e-35 122
Msa1280150 AT3G61190.1 37.766 188 97 6 1 179 16 192 1.18e-29 108
Msa1280150 AT3G61190.2 37.766 188 97 6 1 179 16 192 1.18e-29 108

Find 41 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATATCATATGTTTCTT+TGG 0.263645 8_3:-18211339 None:intergenic
ATAATAGAGTTAGGAGAAAT+GGG 0.321912 8_3:+18211155 Msa1280150:CDS
GATAATAGAGTTAGGAGAAA+TGG 0.337385 8_3:+18211154 Msa1280150:CDS
GTGAGTGGAGTTCCGGTAAC+CGG 0.370119 8_3:+18211247 Msa1280150:CDS
GTCGATGGTGAGTGGAGTTC+CGG 0.410987 8_3:+18211240 Msa1280150:CDS
TAACAGGAATTCCAGCTGTT+TGG 0.411065 8_3:+18211308 Msa1280150:CDS
TCATGTAGCGGAGTTGTAAC+AGG 0.421560 8_3:+18211292 Msa1280150:CDS
CACATACCCTCCAAGAAAAT+CGG 0.422233 8_3:-18211092 None:intergenic
AGTTCCGGTAACCGGGGTTC+CGG 0.425765 8_3:+18211255 Msa1280150:CDS
GTCACCGGAACCCCGGTTAC+CGG 0.431972 8_3:-18211259 None:intergenic
TCCGCTACATGAGCTATTAC+CGG 0.458069 8_3:-18211281 None:intergenic
TGTAGGGTAACTTCCGCCTT+CGG 0.459071 8_3:-18210951 None:intergenic
GGTTGTGGGATAATAGAGTT+AGG 0.466052 8_3:+18211146 Msa1280150:CDS
TGGTAGTTTAACCAAACAGC+TGG 0.466544 8_3:-18211319 None:intergenic
CCTCCAAGAAAATCGGAAAC+AGG 0.468174 8_3:-18211085 None:intergenic
CCAACTTCTCGTTCCATGTA+GGG 0.474471 8_3:-18210967 None:intergenic
GCCTTCGGAATCAAGTTTCG+TGG 0.489360 8_3:-18210936 None:intergenic
GGTAGTAGTAGCAATAGTGT+TGG 0.506913 8_3:+18211049 Msa1280150:CDS
GTAACCGGGGTTCCGGTGAC+CGG 0.511262 8_3:+18211262 Msa1280150:CDS
TGAGTGGAGTTCCGGTAACC+GGG 0.516054 8_3:+18211248 Msa1280150:CDS
GGTTGTAAATATTTCAGTAA+AGG 0.518683 8_3:+18211177 Msa1280150:CDS
ATGGAACGAGAAGTTGGTAA+TGG 0.536894 8_3:+18210973 Msa1280150:CDS
AAGGCGGAAGTTACCCTACA+TGG 0.551053 8_3:+18210954 Msa1280150:CDS
TAGTAGCAATAGTGTTGGAA+TGG 0.552031 8_3:+18211054 Msa1280150:CDS
TCCACTCACCATCGACGACA+TGG 0.556286 8_3:-18211233 None:intergenic
GTGATGAACCTTGCATGTAA+TGG 0.558756 8_3:-18211001 None:intergenic
CCCTACATGGAACGAGAAGT+TGG 0.565961 8_3:+18210967 Msa1280150:CDS
ACCAACTTCTCGTTCCATGT+AGG 0.582262 8_3:-18210968 None:intergenic
TCCATGTCGTCGATGGTGAG+TGG 0.586254 8_3:+18211232 Msa1280150:CDS
CATGAGCTATTACCGGTCAC+CGG 0.597980 8_3:-18211274 None:intergenic
GATGTGAAGTACAAAACAAG+AGG 0.601532 8_3:+18211028 Msa1280150:CDS
TTCGTCACGGTTCAATGTGA+CGG 0.611637 8_3:+18210893 Msa1280150:CDS
TGGATGTGCCATTACATGCA+AGG 0.624126 8_3:+18210993 Msa1280150:CDS
ACGAAACTTGATTCCGAAGG+CGG 0.631770 8_3:+18210938 Msa1280150:CDS
TAATAGAGTTAGGAGAAATG+GGG 0.635926 8_3:+18211156 Msa1280150:CDS
ACCACGAAACTTGATTCCGA+AGG 0.642396 8_3:+18210935 Msa1280150:CDS
ATTACCGGTCACCGGAACCC+CGG 0.657610 8_3:-18211266 None:intergenic
ACCGGTAATAGCTCATGTAG+CGG 0.660755 8_3:+18211280 Msa1280150:CDS
GAGTGGAGTTCCGGTAACCG+GGG 0.677190 8_3:+18211249 Msa1280150:CDS
AAAAGGGAACACATTCGTCA+CGG 0.677311 8_3:+18210880 Msa1280150:CDS
TCAATGTGACGGAAGCAATG+AGG 0.684521 8_3:+18210904 Msa1280150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGCAATTTTTAAGTTAT+AGG + chr8_3:18211125-18211144 Msa1280150:CDS 15.0%
!! AGAATAAAAAAGCCATAAAA+GGG + chr8_3:18210864-18210883 Msa1280150:CDS 20.0%
!!! AATTTTTAAGTTATAGGTTG+TGG + chr8_3:18211131-18211150 Msa1280150:CDS 20.0%
!!! ATTTTTAAGTTATAGGTTGT+GGG + chr8_3:18211132-18211151 Msa1280150:CDS 20.0%
! GAGAATAAAAAAGCCATAAA+AGG + chr8_3:18210863-18210882 Msa1280150:CDS 25.0%
! ATAATAGAGTTAGGAGAAAT+GGG + chr8_3:18211155-18211174 Msa1280150:CDS 25.0%
! GGTTGTAAATATTTCAGTAA+AGG + chr8_3:18211177-18211196 Msa1280150:CDS 25.0%
GATAATAGAGTTAGGAGAAA+TGG + chr8_3:18211154-18211173 Msa1280150:CDS 30.0%
TAATAGAGTTAGGAGAAATG+GGG + chr8_3:18211156-18211175 Msa1280150:CDS 30.0%
GATGTGAAGTACAAAACAAG+AGG + chr8_3:18211028-18211047 Msa1280150:CDS 35.0%
! TAGTAGCAATAGTGTTGGAA+TGG + chr8_3:18211054-18211073 Msa1280150:CDS 35.0%
! GACGAATGTGTTCCCTTTTA+TGG - chr8_3:18210879-18210898 None:intergenic 40.0%
AAAAGGGAACACATTCGTCA+CGG + chr8_3:18210880-18210899 Msa1280150:CDS 40.0%
!! ATGGAACGAGAAGTTGGTAA+TGG + chr8_3:18210973-18210992 Msa1280150:CDS 40.0%
GTGATGAACCTTGCATGTAA+TGG - chr8_3:18211004-18211023 None:intergenic 40.0%
GGTAGTAGTAGCAATAGTGT+TGG + chr8_3:18211049-18211068 Msa1280150:CDS 40.0%
! GTACCTGTTTCCGATTTTCT+TGG + chr8_3:18211082-18211101 Msa1280150:CDS 40.0%
! CTGTTTCCGATTTTCTTGGA+GGG + chr8_3:18211086-18211105 Msa1280150:CDS 40.0%
CACATACCCTCCAAGAAAAT+CGG - chr8_3:18211095-18211114 None:intergenic 40.0%
GGTTGTGGGATAATAGAGTT+AGG + chr8_3:18211146-18211165 Msa1280150:CDS 40.0%
TAACAGGAATTCCAGCTGTT+TGG + chr8_3:18211308-18211327 Msa1280150:CDS 40.0%
TGGTAGTTTAACCAAACAGC+TGG - chr8_3:18211322-18211341 None:intergenic 40.0%
TTCGTCACGGTTCAATGTGA+CGG + chr8_3:18210893-18210912 Msa1280150:CDS 45.0%
! TCAATGTGACGGAAGCAATG+AGG + chr8_3:18210904-18210923 Msa1280150:CDS 45.0%
ACCACGAAACTTGATTCCGA+AGG + chr8_3:18210935-18210954 Msa1280150:CDS 45.0%
ACGAAACTTGATTCCGAAGG+CGG + chr8_3:18210938-18210957 Msa1280150:CDS 45.0%
CCAACTTCTCGTTCCATGTA+GGG - chr8_3:18210970-18210989 None:intergenic 45.0%
ACCAACTTCTCGTTCCATGT+AGG - chr8_3:18210971-18210990 None:intergenic 45.0%
! TGGATGTGCCATTACATGCA+AGG + chr8_3:18210993-18211012 Msa1280150:CDS 45.0%
CCTCCAAGAAAATCGGAAAC+AGG - chr8_3:18211088-18211107 None:intergenic 45.0%
! CCTGTTTCCGATTTTCTTGG+AGG + chr8_3:18211085-18211104 Msa1280150:CDS 45.0%
ACCGGTAATAGCTCATGTAG+CGG + chr8_3:18211280-18211299 Msa1280150:CDS 45.0%
TCCGCTACATGAGCTATTAC+CGG - chr8_3:18211284-18211303 None:intergenic 45.0%
! TCATGTAGCGGAGTTGTAAC+AGG + chr8_3:18211292-18211311 Msa1280150:CDS 45.0%
GCCTTCGGAATCAAGTTTCG+TGG - chr8_3:18210939-18210958 None:intergenic 50.0%
TGTAGGGTAACTTCCGCCTT+CGG - chr8_3:18210954-18210973 None:intergenic 50.0%
AAGGCGGAAGTTACCCTACA+TGG + chr8_3:18210954-18210973 Msa1280150:CDS 50.0%
CCCTACATGGAACGAGAAGT+TGG + chr8_3:18210967-18210986 Msa1280150:CDS 50.0%
CATGAGCTATTACCGGTCAC+CGG - chr8_3:18211277-18211296 None:intergenic 50.0%
CTCGAGTTCCATGTCGTCGA+TGG + chr8_3:18211225-18211244 Msa1280150:CDS 55.0%
TCCATGTCGTCGATGGTGAG+TGG + chr8_3:18211232-18211251 Msa1280150:CDS 55.0%
TCCACTCACCATCGACGACA+TGG - chr8_3:18211236-18211255 None:intergenic 55.0%
GTCGATGGTGAGTGGAGTTC+CGG + chr8_3:18211240-18211259 Msa1280150:CDS 55.0%
GTGAGTGGAGTTCCGGTAAC+CGG + chr8_3:18211247-18211266 Msa1280150:CDS 55.0%
TGAGTGGAGTTCCGGTAACC+GGG + chr8_3:18211248-18211267 Msa1280150:CDS 55.0%
GAGTGGAGTTCCGGTAACCG+GGG + chr8_3:18211249-18211268 Msa1280150:CDS 60.0%
AGTTCCGGTAACCGGGGTTC+CGG + chr8_3:18211255-18211274 Msa1280150:CDS 60.0%
ATTACCGGTCACCGGAACCC+CGG - chr8_3:18211269-18211288 None:intergenic 60.0%
GTCACCGGAACCCCGGTTAC+CGG - chr8_3:18211262-18211281 None:intergenic 65.0%
GTAACCGGGGTTCCGGTGAC+CGG + chr8_3:18211262-18211281 Msa1280150:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 18210815 18211354 18210815 ID=Msa1280150;Name=Msa1280150
chr8_3 mRNA 18210815 18211354 18210815 ID=Msa1280150-mRNA-1;Parent=Msa1280150;Name=Msa1280150-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|179
chr8_3 exon 18210815 18211354 18210815 ID=Msa1280150-mRNA-1:exon:6912;Parent=Msa1280150-mRNA-1
chr8_3 CDS 18210815 18211354 18210815 ID=Msa1280150-mRNA-1:cds;Parent=Msa1280150-mRNA-1
Gene Sequence

>Msa1280150

ATGTCACGAACAATAGAGATAACAATTTTGTCAGCAGAGAATCTTCAAGAGAATAAAAAAGCCATAAAAGGGAACACATTCGTCACGGTTCAATGTGACGGAAGCAATGAGGAAGTGAGCACCACGAAACTTGATTCCGAAGGCGGAAGTTACCCTACATGGAACGAGAAGTTGGTAATGGATGTGCCATTACATGCAAGGTTCATCACAATAGATGTGAAGTACAAAACAAGAGGTAGTAGTAGCAATAGTGTTGGAATGGCTAGAGTACCTGTTTCCGATTTTCTTGGAGGGTATGTGCATGAAAATCAATTGCAATTTTTAAGTTATAGGTTGTGGGATAATAGAGTTAGGAGAAATGGGGTTGTAAATATTTCAGTAAAGGTAAAAATGTCGCAACAAAATTCTTGCTCGAGTTCCATGTCGTCGATGGTGAGTGGAGTTCCGGTAACCGGGGTTCCGGTGACCGGTAATAGCTCATGTAGCGGAGTTGTAACAGGAATTCCAGCTGTTTGGTTAAACTACCAAAGAAACATATGA

Protein sequence

>Msa1280150

MSRTIEITILSAENLQENKKAIKGNTFVTVQCDGSNEEVSTTKLDSEGGSYPTWNEKLVMDVPLHARFITIDVKYKTRGSSSNSVGMARVPVSDFLGGYVHENQLQFLSYRLWDNRVRRNGVVNISVKVKMSQQNSCSSSMSSMVSGVPVTGVPVTGNSSCSGVVTGIPAVWLNYQRNI*