AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1285450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1285450 MtrunA17_Chr8g0360521 97.460 315 7 1 1 314 103 417 0.0 645
Msa1285450 MtrunA17_Chr3g0113671 50.641 312 148 4 1 306 138 449 1.05e-111 331
Msa1285450 MtrunA17_Chr4g0006991 49.196 311 152 3 1 306 118 427 1.62e-111 330
Msa1285450 MtrunA17_Chr5g0437601 49.201 313 149 5 2 307 116 425 3.25e-110 326
Msa1285450 MtrunA17_Chr7g0242701 50.482 311 148 3 1 306 118 427 1.41e-109 325
Msa1285450 MtrunA17_Chr4g0005491 50.476 315 146 6 2 307 95 408 7.28e-109 322
Msa1285450 MtrunA17_Chr3g0114101 49.201 313 149 5 2 307 122 431 3.40e-108 322
Msa1285450 MtrunA17_Chr7g0243301 47.152 316 155 7 2 307 97 410 1.86e-103 308
Msa1285450 MtrunA17_Chr4g0005481 47.557 307 158 3 2 306 141 446 4.30e-102 306
Msa1285450 MtrunA17_Chr5g0437291 45.253 316 164 5 1 307 197 512 1.26e-98 300
Msa1285450 MtrunA17_Chr1g0171171 41.346 312 156 8 2 306 84 375 2.09e-73 230
Msa1285450 MtrunA17_Chr3g0105301 38.782 312 172 6 4 307 77 377 8.65e-73 229
Msa1285450 MtrunA17_Chr1g0210771 39.159 309 168 8 2 306 90 382 1.48e-71 226
Msa1285450 MtrunA17_Chr5g0430371 35.514 321 180 8 3 307 91 400 6.08e-68 217
Msa1285450 MtrunA17_Chr7g0258851 35.404 322 181 8 1 307 279 588 4.34e-64 212
Msa1285450 MtrunA17_Chr1g0175671 34.783 322 183 8 1 307 201 510 2.50e-63 209
Msa1285450 MtrunA17_Chr2g0314801 36.928 306 166 7 3 303 67 350 3.43e-63 203
Msa1285450 MtrunA17_Chr4g0068111 34.835 333 179 8 1 313 146 460 4.28e-63 206
Msa1285450 MtrunA17_Chr3g0097891 36.102 313 167 7 3 307 64 351 5.50e-63 203
Msa1285450 MtrunA17_Chr4g0071391 35.016 317 181 11 3 306 142 446 1.85e-61 202
Msa1285450 MtrunA17_Chr2g0287901 39.571 326 163 14 2 308 129 439 1.44e-60 199
Msa1285450 MtrunA17_Chr5g0413271 35.220 318 177 8 3 308 178 478 2.05e-60 200
Msa1285450 MtrunA17_Chr7g0259711 35.976 328 173 12 2 308 123 434 3.04e-60 199
Msa1285450 MtrunA17_Chr7g0269991 34.365 323 168 10 3 306 170 467 1.05e-59 198
Msa1285450 MtrunA17_Chr2g0283581 36.129 310 169 8 2 306 73 358 1.16e-58 192
Msa1285450 MtrunA17_Chr3g0085081 36.422 313 166 8 1 306 62 348 1.18e-58 192
Msa1285450 MtrunA17_Chr4g0041011 34.286 315 167 9 2 306 79 363 4.24e-58 191
Msa1285450 MtrunA17_Chr3g0091031 36.102 313 167 8 1 306 62 348 2.90e-57 188
Msa1285450 MtrunA17_Chr2g0314791 33.016 315 173 8 1 309 66 348 5.88e-57 187
Msa1285450 MtrunA17_Chr4g0045941 33.550 307 179 6 4 307 67 351 3.35e-56 185
Msa1285450 MtrunA17_Chr2g0325481 34.194 310 176 8 2 307 82 367 7.25e-56 185
Msa1285450 MtrunA17_Chr5g0413851 35.669 314 169 7 4 309 74 362 2.17e-55 184
Msa1285450 MtrunA17_Chr1g0213281 31.861 317 196 9 3 307 208 516 4.10e-54 184
Msa1285450 MtrunA17_Chr4g0045951 33.333 309 181 6 2 307 77 363 7.89e-54 179
Msa1285450 MtrunA17_Chr3g0085071 33.974 312 175 8 1 306 77 363 1.20e-53 179
Msa1285450 MtrunA17_Chr8g0368461 32.132 333 186 9 3 308 211 530 6.25e-50 173
Msa1285450 MtrunA17_Chr3g0126021 31.975 319 181 8 3 308 166 461 1.78e-49 171
Msa1285450 MtrunA17_Chr8g0384151 31.481 324 186 9 1 311 121 421 6.80e-49 168
Msa1285450 MtrunA17_Chr1g0209921 34.010 197 121 5 1 195 77 266 6.24e-39 138
Msa1285450 MtrunA17_Chr1g0178081 28.882 322 159 7 2 314 89 349 7.48e-37 135
Msa1285450 MtrunA17_Chr7g0226991 28.704 324 204 11 3 308 90 404 6.88e-36 133
Msa1285450 MtrunA17_Chr8g0391481 28.698 338 197 10 3 314 60 379 7.68e-35 130
Msa1285450 MtrunA17_Chr6g0456691 28.313 332 191 11 1 306 109 419 8.54e-34 128
Msa1285450 MtrunA17_Chr5g0414091 27.463 335 187 13 1 302 116 427 3.25e-33 127
Msa1285450 MtrunA17_Chr1g0188191 27.859 341 199 15 3 314 267 589 6.36e-33 127
Msa1285450 MtrunA17_Chr8g0347321 28.963 328 197 13 1 306 126 439 8.97e-33 125
Msa1285450 MtrunA17_Chr6g0476041 27.193 342 196 12 3 312 200 520 9.36e-33 126
Msa1285450 MtrunA17_Chr5g0414051 27.729 339 192 12 1 308 106 422 5.25e-32 123
Msa1285450 MtrunA17_Chr3g0140371 26.667 330 201 9 3 308 50 362 7.13e-32 122
Msa1285450 MtrunA17_Chr2g0283851 29.969 327 183 15 4 302 88 396 3.24e-31 120
Msa1285450 MtrunA17_Chr8g0384421 28.395 324 190 11 5 306 169 472 6.70e-31 120
Msa1285450 MtrunA17_Chr5g0414061 28.235 340 188 11 1 308 125 440 3.83e-30 118
Msa1285450 MtrunA17_Chr6g0456681 27.640 322 201 11 1 302 95 404 1.58e-29 116
Msa1285450 MtrunA17_Chr5g0414041 26.667 330 208 8 1 308 118 435 3.75e-28 113
Msa1285450 MtrunA17_Chr8g0387921 28.916 332 185 16 1 302 105 415 4.07e-28 112
Msa1285450 MtrunA17_Chr4g0070341 27.507 349 161 16 5 306 168 471 6.69e-26 107
Msa1285450 MtrunA17_Chr4g0049421 27.059 340 204 16 1 314 79 400 6.21e-25 103
Msa1285450 MtrunA17_Chr6g0455901 25.826 333 194 14 3 306 108 416 5.12e-23 98.6
Msa1285450 MtrunA17_Chr6g0451321 24.012 329 208 14 1 306 148 457 2.43e-20 91.3
Msa1285450 MtrunA17_Chr7g0221291 54.098 61 28 0 1 61 110 170 1.25e-18 81.6
Msa1285450 MtrunA17_Chr7g0221271 54.098 61 28 0 1 61 110 170 5.31e-18 80.9
Msa1285450 MtrunA17_Chr2g0287931 32.540 126 76 3 30 147 2 126 1.26e-15 72.4
Msa1285450 MtrunA17_Chr3g0085101 37.736 106 57 3 163 264 6 106 3.03e-14 68.2
Msa1285450 MtrunA17_Chr5g0429191 61.224 49 13 2 252 299 2 45 1.79e-12 61.2
Msa1285450 MtrunA17_Chr1g0209931 34.286 105 52 2 205 309 8 95 2.65e-12 62.4
Msa1285450 MtrunA17_Chr5g0414081 29.197 137 71 3 185 308 78 201 6.58e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1285450 AT1G73140.1 73.482 313 82 1 2 313 100 412 1.47e-180 505
Msa1285450 AT5G01360.2 51.125 311 147 2 1 306 68 378 6.29e-118 345
Msa1285450 AT5G01360.1 51.125 311 147 2 1 306 124 434 4.22e-117 345
Msa1285450 AT3G55990.1 50.479 313 149 3 1 307 172 484 1.17e-114 340
Msa1285450 AT2G40150.1 50.000 318 147 3 1 306 103 420 5.42e-114 336
Msa1285450 AT2G40320.1 47.604 313 154 4 2 307 114 423 2.13e-105 314
Msa1285450 AT3G11030.1 48.875 311 152 5 3 307 140 449 1.28e-104 313
Msa1285450 AT5G01620.3 44.805 308 165 4 2 306 152 457 8.03e-101 304
Msa1285450 AT5G01620.2 44.805 308 165 4 2 306 144 449 1.01e-100 303
Msa1285450 AT5G01620.1 44.805 308 165 4 2 306 144 449 1.01e-100 303
Msa1285450 AT2G40160.1 46.349 315 160 4 2 307 110 424 2.45e-100 301
Msa1285450 AT2G40160.2 46.349 315 160 4 2 307 110 424 3.47e-100 301
Msa1285450 AT2G38320.2 47.022 319 157 6 2 310 9 325 3.15e-98 293
Msa1285450 AT2G38320.1 47.022 319 157 6 2 310 92 408 2.76e-97 293
Msa1285450 AT3G54260.1 39.228 311 171 5 2 309 82 377 2.07e-73 231
Msa1285450 AT5G58600.1 35.691 311 183 5 4 307 101 401 5.34e-67 215
Msa1285450 AT2G30900.1 37.380 313 166 10 3 307 76 366 5.86e-66 211
Msa1285450 AT3G12060.1 36.335 322 178 8 1 307 227 536 9.56e-66 216
Msa1285450 AT5G20590.1 35.938 320 178 10 2 307 174 480 2.40e-65 213
Msa1285450 AT3G62390.1 36.364 319 172 10 3 307 171 472 3.30e-65 213
Msa1285450 AT5G06700.1 36.875 320 179 10 1 307 286 595 1.29e-63 211
Msa1285450 AT2G40160.3 47.059 204 101 2 2 198 110 313 1.40e-63 204
Msa1285450 AT1G78710.1 35.048 311 175 9 2 307 69 357 2.99e-63 204
Msa1285450 AT5G49340.1 36.858 331 166 10 1 307 131 442 1.21e-62 205
Msa1285450 AT2G30010.1 34.365 323 176 8 4 307 92 397 4.21e-62 202
Msa1285450 AT2G30010.2 34.365 323 176 8 4 307 135 440 8.18e-62 203
Msa1285450 AT2G31110.2 37.217 309 167 8 2 306 77 362 4.23e-61 199
Msa1285450 AT1G29050.1 34.839 310 175 7 1 306 92 378 2.44e-59 195
Msa1285450 AT2G34070.1 33.762 311 178 7 1 306 96 383 4.08e-59 194
Msa1285450 AT3G06080.2 35.826 321 180 8 4 308 137 447 9.63e-59 196
Msa1285450 AT1G60790.1 34.365 323 182 10 3 308 225 534 1.18e-57 194
Msa1285450 AT2G42570.1 35.484 310 173 6 2 307 79 365 2.60e-56 186
Msa1285450 AT5G19160.1 33.133 332 188 10 4 314 133 451 1.85e-54 184
Msa1285450 AT5G06230.2 36.420 324 180 8 2 311 59 370 3.05e-54 181
Msa1285450 AT3G14850.2 33.333 312 182 8 2 308 66 356 3.20e-54 181
Msa1285450 AT5G06230.1 36.420 324 180 8 2 311 100 411 9.78e-54 181
Msa1285450 AT3G11570.1 33.929 336 174 11 1 311 113 425 2.14e-50 172
Msa1285450 AT1G48880.1 30.745 322 183 11 1 308 146 441 1.53e-46 163
Msa1285450 AT2G34070.2 32.353 272 156 7 40 306 1 249 2.52e-46 157
Msa1285450 AT1G78710.2 29.412 306 146 7 2 307 62 297 1.11e-44 154
Msa1285450 AT3G14850.1 31.752 274 161 8 40 308 1 253 1.21e-40 142
Msa1285450 AT5G20590.2 34.951 206 121 5 2 196 174 377 8.43e-39 141
Msa1285450 AT5G58600.2 34.359 195 119 2 4 195 101 289 2.53e-38 137
Msa1285450 AT3G62390.2 34.314 204 122 5 3 196 171 372 4.87e-38 140
Msa1285450 AT2G31110.3 36.458 192 112 3 2 190 77 261 7.94e-38 135
Msa1285450 AT2G30900.2 37.500 192 106 6 3 188 76 259 1.43e-37 135
Msa1285450 AT5G64020.1 29.909 331 190 12 4 306 92 408 7.59e-37 136
Msa1285450 AT3G06080.1 34.483 203 123 3 4 196 137 339 6.62e-36 132
Msa1285450 AT5G19160.2 33.005 203 126 3 4 196 133 335 1.30e-35 132
Msa1285450 AT1G70230.1 26.266 316 213 10 1 308 110 413 3.18e-35 132
Msa1285450 AT5G06230.3 36.408 206 117 4 2 195 100 303 7.78e-34 126
Msa1285450 AT2G37720.1 29.499 339 169 14 4 306 178 482 8.12e-34 129
Msa1285450 AT2G31110.1 35.345 232 123 8 79 306 6 214 1.05e-33 123
Msa1285450 AT5G64470.3 28.182 330 201 14 1 311 87 399 4.11e-33 126
Msa1285450 AT3G02440.1 28.528 326 198 11 1 306 158 468 1.06e-32 126
Msa1285450 AT3G02440.2 28.528 326 198 11 1 306 166 476 1.08e-32 126
Msa1285450 AT4G25360.1 27.405 343 200 13 3 314 208 532 2.45e-32 126
Msa1285450 AT4G25360.2 27.405 343 200 13 3 314 208 532 2.45e-32 126
Msa1285450 AT5G20680.6 27.697 343 168 13 5 306 154 457 3.98e-32 124
Msa1285450 AT5G15890.1 29.245 318 192 9 1 302 218 518 8.32e-32 124
Msa1285450 AT5G20680.2 27.697 343 168 13 5 306 230 533 9.90e-32 124
Msa1285450 AT5G20680.4 27.697 343 168 13 5 306 248 551 1.13e-31 124
Msa1285450 AT5G20680.1 27.697 343 168 13 5 306 248 551 1.13e-31 124
Msa1285450 AT5G20680.3 27.697 343 168 13 5 306 248 551 1.13e-31 124
Msa1285450 AT5G20680.5 27.697 343 168 13 5 306 248 551 1.13e-31 124
Msa1285450 AT2G14530.1 28.274 336 188 14 1 306 94 406 1.17e-31 122
Msa1285450 AT5G51640.1 27.168 346 194 13 3 312 171 494 4.38e-31 122
Msa1285450 AT5G64470.2 27.679 336 201 16 1 311 87 405 4.25e-29 115
Msa1285450 AT5G15900.1 27.829 327 200 11 1 306 98 409 5.27e-28 112
Msa1285450 AT5G15900.2 27.829 327 200 11 1 306 117 428 7.47e-28 112
Msa1285450 AT1G01430.1 26.586 331 207 12 1 307 130 448 3.16e-27 110
Msa1285450 AT3G28150.1 28.869 336 183 16 1 306 101 410 2.47e-26 108
Msa1285450 AT4G01080.1 26.074 326 196 13 1 302 122 426 3.39e-26 107
Msa1285450 AT4G11090.1 24.848 330 201 12 1 302 111 421 5.01e-26 107
Msa1285450 AT4G23790.1 25.507 345 182 16 1 302 111 423 8.31e-26 106
Msa1285450 AT5G64470.1 25.000 200 132 7 11 196 98 293 4.21e-13 69.3
Msa1285450 AT5G64470.4 25.126 199 131 7 12 196 99 293 5.05e-13 68.9

Find 137 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCAATCAGCAAAAGATTT+TGG 0.125911 8_3:-29993737 None:intergenic
AGAAGTGAATACAATATAAT+TGG 0.171993 8_3:-29991561 None:intergenic
AGGATTTCATTCCATGTATC+TGG 0.201338 8_3:-29993782 None:intergenic
GATGCTCACACCTCAGTTTA+TGG 0.206651 8_3:+29993671 Msa1285450:CDS
AACAAAAGAATGATGTTTAT+TGG 0.212670 8_3:+29992071 Msa1285450:CDS
CTGATTCTTTCTACACAAAT+TGG 0.216029 8_3:+29991834 Msa1285450:CDS
CAATACAAGGTTCGTCATAT+TGG 0.230048 8_3:+29993540 Msa1285450:CDS
TAGCTCTCTGTTCTTTAGTT+AGG 0.246525 8_3:-29993712 None:intergenic
TGGAGGGATTTCTTTCCTTC+AGG 0.275411 8_3:-29992152 None:intergenic
TCTTCATAGGTGGAATTCTT+TGG 0.275642 8_3:-29992172 None:intergenic
CTGTCGTGACGTTATATTCT+TGG 0.282711 8_3:-29992952 None:intergenic
AATACAAGGTTCGTCATATT+GGG 0.282741 8_3:+29993541 Msa1285450:CDS
CCTTATACGATCTAAGCTTT+TGG 0.289384 8_3:+29994770 Msa1285450:intron
TGCAATGTATTTGAGGGAAA+TGG 0.304967 8_3:+29991723 Msa1285450:five_prime_UTR
TTGGCAAGGAGTGGACATTT+TGG 0.310618 8_3:+29992664 Msa1285450:CDS
TGCATTCACTGGTGTTTACC+TGG 0.316704 8_3:+29993755 Msa1285450:CDS
AACAGATCATAGACTCAAAT+TGG 0.319491 8_3:-29992112 None:intergenic
GCTTGCTCTTCAAGCTATCT+AGG 0.322912 8_3:-29991608 None:intergenic
ATACTACTGGGCTCCATTCA+TGG 0.353905 8_3:+29992556 Msa1285450:CDS
CGTTGAGTTCACGTATGTTC+TGG 0.354464 8_3:+29994237 Msa1285450:three_prime_UTR
TCAACTTTAAATATCTTCAT+AGG 0.360087 8_3:-29992185 None:intergenic
ATATAATTGGGAAAATTGAT+TGG 0.370417 8_3:-29991548 None:intergenic
TGCCGAAAATTATATTGTTT+AGG 0.375449 8_3:-29994083 None:intergenic
CAGCTCTACGGCATGTCATC+TGG 0.379386 8_3:+29991645 Msa1285450:five_prime_UTR
TTATATTCTTGGACTTCATC+TGG 0.380050 8_3:-29992941 None:intergenic
TAACTTACCACAAATGTGTT+GGG 0.386349 8_3:-29993063 None:intergenic
ATGCATCGATTGAATACTAC+TGG 0.392477 8_3:+29992543 Msa1285450:CDS
ATTTGAGTCTATGATCTGTT+TGG 0.397442 8_3:+29992115 Msa1285450:CDS
ATAATTGGGTGATGTTCAAT+AGG 0.397627 8_3:-29993634 None:intergenic
CAATATGACGAACCTTGTAT+TGG 0.400765 8_3:-29993539 None:intergenic
AGTATGAACAACTAGCAATA+TGG 0.407962 8_3:-29994321 Msa1285460:CDS
GTGGTCTGCTTGACCAAGTA+AGG 0.408960 8_3:-29991791 None:intergenic
TTTAGGTTTCCATGAAGAAC+AGG 0.415460 8_3:-29994066 None:intergenic
AGAAGTGAGTTGCCCTTACT+TGG 0.416247 8_3:+29991778 Msa1285450:five_prime_UTR
GCAATGTATTTGAGGGAAAT+GGG 0.419224 8_3:+29991724 Msa1285450:five_prime_UTR
ACGCTCTATTAAATTAAAGT+TGG 0.428012 8_3:-29994881 Msa1285460:CDS
TGGTGGAAGGAATCGATTAC+CGG 0.428048 8_3:+29991665 Msa1285450:five_prime_UTR
AGGCTAAAATCATAAACTAT+AGG 0.429225 8_3:-29994046 None:intergenic
CAAATCATACGGTACATAAA+CGG 0.437106 8_3:+29992603 Msa1285450:CDS
CAATTTGTGTAGAAAGAATC+AGG 0.437639 8_3:-29991833 None:intergenic
ATTACCGGTTGTTGTGTTGC+CGG 0.437991 8_3:+29991680 Msa1285450:five_prime_UTR
TCTTAAACAGATATGCATAT+AGG 0.438576 8_3:-29993802 None:intergenic
GAAGTGAATACAATATAATT+GGG 0.442470 8_3:-29991560 None:intergenic
GAACAACTAGCAATATGGTT+TGG 0.449984 8_3:-29994316 Msa1285460:CDS
GAAATTGATTCAACCATGAA+TGG 0.451055 8_3:-29992569 None:intergenic
TTGGGAAAATTGATTGGATC+CGG 0.452959 8_3:-29991542 None:intergenic
AATATGACGAACCTTGTATT+GGG 0.455104 8_3:-29993538 None:intergenic
ACAAGCATCATTCAGATTGA+TGG 0.455770 8_3:+29993872 Msa1285450:three_prime_UTR
CCCTCCTCAATGCGATCCTC+CGG 0.457796 8_3:-29991699 None:intergenic
TTCTAATCTTTGCAGTTACA+AGG 0.466052 8_3:+29991580 Msa1285450:five_prime_UTR
CAGACCACTTGCATGAAGAA+TGG 0.466368 8_3:+29991806 Msa1285450:exon
TCCTCCGGCAACACAACAAC+CGG 0.467189 8_3:-29991684 None:intergenic
GGAGGGTTGCAATGTATTTG+AGG 0.469634 8_3:+29991716 Msa1285450:five_prime_UTR
GCATTCACTGGTGTTTACCT+GGG 0.470418 8_3:+29993756 Msa1285450:CDS
CTCACGTAGCATTGATGAAA+GGG 0.471007 8_3:-29992718 None:intergenic
GTATTTGAGGGAAATGGGTA+TGG 0.471646 8_3:+29991729 Msa1285450:five_prime_UTR
CTCTATTAAATTAAAGTTGG+AGG 0.475359 8_3:-29994878 Msa1285460:CDS
AGACCACTTGCATGAAGAAT+GGG 0.475855 8_3:+29991807 Msa1285450:exon
AGGAGCTGGGAATGGAAGCC+AGG 0.483811 8_3:+29993485 Msa1285450:intron
GGAGATGCTGATATCGAGCC+AGG 0.490893 8_3:-29994295 Msa1285460:CDS
ATCCAAAAGGCAAAAGGTGT+TGG 0.491457 8_3:-29993947 None:intergenic
CATAGGTGGAATTCTTTGGA+GGG 0.491727 8_3:-29992168 None:intergenic
CGGTATATTTGTGAATGATC+GGG 0.491737 8_3:-29994818 Msa1285460:CDS
CTCAGTTTATGGCGAGCGAA+AGG 0.496603 8_3:+29993682 Msa1285450:CDS
GCGGTATATTTGTGAATGAT+CGG 0.497341 8_3:-29994819 Msa1285460:CDS
GAGGGTTGCAATGTATTTGA+GGG 0.504839 8_3:+29991717 Msa1285450:five_prime_UTR
ATTCTTTCTACACAAATTGG+AGG 0.504866 8_3:+29991837 Msa1285450:CDS
CATGGGACAGAAAAGACTCC+AGG 0.506317 8_3:-29994377 Msa1285460:intron
CATTGATATGAGATGGTTGA+AGG 0.506584 8_3:-29991493 None:intergenic
GCCGGAGGATCGCATTGAGG+AGG 0.515226 8_3:+29991698 Msa1285450:five_prime_UTR
TCTTCTCCAGGAGCTGGGAA+TGG 0.516727 8_3:+29993477 Msa1285450:intron
CAACTCACTTCTTCATAAAG+AGG 0.520659 8_3:-29991767 None:intergenic
TATACAATCCAAAAGGCAAA+AGG 0.521640 8_3:-29993953 None:intergenic
TCATAGGTGGAATTCTTTGG+AGG 0.523486 8_3:-29992169 None:intergenic
GGATTTCATTCCATGTATCT+GGG 0.524709 8_3:-29993781 None:intergenic
GAAAAGAAAGCAAAATAGAA+TGG 0.525233 8_3:-29993925 None:intergenic
GTAACTTACCACAAATGTGT+TGG 0.530098 8_3:-29993064 None:intergenic
TACCTGGGGTCCCAGATACA+TGG 0.530934 8_3:+29993771 Msa1285450:CDS
AATTGGAGGTGGCAGCCTCA+TGG 0.531507 8_3:+29991851 Msa1285450:CDS
GTTGCAAATGTACATGTCAA+AGG 0.533678 8_3:-29994116 None:intergenic
TTCCATGTATCTGGGACCCC+AGG 0.534611 8_3:-29993773 None:intergenic
GGTTCGTCATATTGGGGCAC+AGG 0.534995 8_3:+29993548 Msa1285450:CDS
TCAGTTTATGGCGAGCGAAA+GGG 0.538195 8_3:+29993683 Msa1285450:CDS
TCTTCGAAGAGAATGCATCC+TGG 0.541502 8_3:+29994359 Msa1285450:three_prime_UTR
GTTCTGTTATACAATCCAAA+AGG 0.542969 8_3:-29993960 None:intergenic
GAATTAAATCGTCTGAGAGT+TGG 0.543056 8_3:+29994259 Msa1285450:three_prime_UTR
TGTTCGAGAGCTATGTTTGG+TGG 0.543784 8_3:+29992687 Msa1285450:CDS
CATTCACTGGTGTTTACCTG+GGG 0.545869 8_3:+29993757 Msa1285450:CDS
TGGTGTTCGAGAGCTATGTT+TGG 0.547200 8_3:+29992684 Msa1285450:CDS
CTTTCTACACAAATTGGAGG+TGG 0.550918 8_3:+29991840 Msa1285450:CDS
GTACAATCTGTAATTCCTGA+AGG 0.559209 8_3:+29992137 Msa1285450:CDS
TCTCCCATTCTTCATGCAAG+TGG 0.561570 8_3:-29991810 None:intergenic
TATGAAGATATTTAAAGTTG+AGG 0.563429 8_3:+29992187 Msa1285450:CDS
ATGCATTCTCTTCGAAGAAG+AGG 0.567019 8_3:-29994354 Msa1285460:CDS
AGCAAGCTTCTGAAACTGAG+TGG 0.568778 8_3:-29994201 Msa1285460:CDS
TAATTGGGTGATGTTCAATA+GGG 0.568867 8_3:-29993633 None:intergenic
TTAAATCGTCTGAGAGTTGG+TGG 0.569032 8_3:+29994262 Msa1285450:three_prime_UTR
CCGGAGGATCGCATTGAGGA+GGG 0.569780 8_3:+29991699 Msa1285450:five_prime_UTR
ACTCACGTAGCATTGATGAA+AGG 0.570089 8_3:-29992719 None:intergenic
AACATGTGCAACAACTTCAA+AGG 0.571932 8_3:-29992044 None:intergenic
TAAATAAAACACAAATGTTG+TGG 0.572067 8_3:-29994147 None:intergenic
TAGCTAAGCATGGCAAACAT+TGG 0.579693 8_3:+29992645 Msa1285450:CDS
GCAAGCTTCTGAAACTGAGT+GGG 0.583735 8_3:-29994200 Msa1285460:CDS
TGAAGTTGTTGCACATGTTG+AGG 0.585215 8_3:+29992048 Msa1285450:CDS
CTCATGGATGCAACCTCCCA+AGG 0.585468 8_3:+29991867 Msa1285450:CDS
TATTTGAGGGAAATGGGTAT+GGG 0.586741 8_3:+29991730 Msa1285450:five_prime_UTR
CGTCACGACAGCATATAAGT+TGG 0.586816 8_3:+29992964 Msa1285450:CDS
TCAAGTCTTCGATTGATCGC+CGG 0.588728 8_3:+29991523 Msa1285450:five_prime_UTR
TTCGAAGAAGAGGTTAGCAA+AGG 0.592548 8_3:-29994344 Msa1285460:CDS
ACTTTAAATATCTTCATAGG+TGG 0.594287 8_3:-29992182 None:intergenic
TGATCATGCAACAAATCATA+CGG 0.594915 8_3:+29992592 Msa1285450:CDS
AACTTACCACAAATGTGTTG+GGG 0.596950 8_3:-29993062 None:intergenic
GCAAATGTACATGTCAAAGG+TGG 0.597773 8_3:-29994113 None:intergenic
ACCGGTTGTTGTGTTGCCGG+AGG 0.598806 8_3:+29991683 Msa1285450:five_prime_UTR
TGGCAAACATTGGCAAGGAG+TGG 0.599503 8_3:+29992655 Msa1285450:CDS
ATTGGAGACTCATTACAAAG+AGG 0.601036 8_3:+29992089 Msa1285450:CDS
ACGGCATGTCATCTGGTGGA+AGG 0.604816 8_3:+29991652 Msa1285450:five_prime_UTR
CACTTCTTCATAAAGAGGAT+AGG 0.612582 8_3:-29991762 None:intergenic
CTTGACTCAATAGCTAAGCA+TGG 0.612907 8_3:+29992635 Msa1285450:CDS
CTCTACGGCATGTCATCTGG+TGG 0.613992 8_3:+29991648 Msa1285450:five_prime_UTR
AGCATCATTCAGATTGATGG+TGG 0.614413 8_3:+29993875 Msa1285450:three_prime_UTR
TGTTGTAAATAATAATCAAG+TGG 0.615423 8_3:-29994174 None:intergenic
GTTGCCGGAGGATCGCATTG+AGG 0.623555 8_3:+29991695 Msa1285450:five_prime_UTR
TCATACGGTACATAAACGGA+TGG 0.623999 8_3:+29992607 Msa1285450:CDS
AATGAATCATACCCAATACA+AGG 0.626400 8_3:+29993527 Msa1285450:CDS
AAAGTGTTGGCTACCTTGGG+AGG 0.628073 8_3:-29991880 None:intergenic
GTTGGTGGTAAATCTTGACC+TGG 0.628676 8_3:+29994277 Msa1285450:three_prime_UTR
CTTGGGAGGTTGCATCCATG+AGG 0.629461 8_3:-29991866 None:intergenic
CAAATGTACATGTCAAAGGT+GGG 0.631598 8_3:-29994112 None:intergenic
GAGATACAAACTGTAAACAA+AGG 0.634145 8_3:+29994006 Msa1285450:three_prime_UTR
TGCATCGATTGAATACTACT+GGG 0.637341 8_3:+29992544 Msa1285450:CDS
AAGCATGGCAAACATTGGCA+AGG 0.639024 8_3:+29992650 Msa1285450:CDS
GAGACAGCATTGATATGAGA+TGG 0.642148 8_3:-29991500 None:intergenic
TGGTCTGCTTGACCAAGTAA+GGG 0.642359 8_3:-29991790 None:intergenic
GATACAAAGCGTGAAGATAG+CGG 0.644538 8_3:-29994838 Msa1285460:CDS
ATACAAGGTTCGTCATATTG+GGG 0.647384 8_3:+29993542 Msa1285450:CDS
CTTTCGCTCGCCATAAACTG+AGG 0.649074 8_3:-29993681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTAAGTTCACAAT+CGG - chr8_3:29991943-29991962 None:intergenic 15.0%
!! TAATAACTCTAGTTATTATT+AGG - chr8_3:29993253-29993272 None:intergenic 15.0%
!!! AAATTCGATTTTTTATTTTC+TGG + chr8_3:29993414-29993433 Msa1285450:intron 15.0%
!!! AACCTAAACAATATAATTTT+CGG + chr8_3:29994081-29994100 Msa1285450:three_prime_UTR 15.0%
!!! CTTGAATTTTATTCAAAAAA+TGG - chr8_3:29994486-29994505 Msa1285460:intron 15.0%
!!! CATTTTTTGAATAAAATTCA+AGG + chr8_3:29994484-29994503 Msa1285450:intron 15.0%
!!! CTATTTTAGTTGTTTTTTTT+TGG + chr8_3:29994566-29994585 Msa1285450:intron 15.0%
!! AGTTAAGTTATTCTATATAT+TGG - chr8_3:29994703-29994722 Msa1285460:intron 15.0%
!! ATATAATTGGGAAAATTGAT+TGG - chr8_3:29991551-29991570 None:intergenic 20.0%
!! GAAGTGAATACAATATAATT+GGG - chr8_3:29991563-29991582 None:intergenic 20.0%
!! AGAAGTGAATACAATATAAT+TGG - chr8_3:29991564-29991583 None:intergenic 20.0%
!! AACAAAAGAATGATGTTTAT+TGG + chr8_3:29992071-29992090 Msa1285450:CDS 20.0%
!! TCAACTTTAAATATCTTCAT+AGG - chr8_3:29992188-29992207 None:intergenic 20.0%
!!! TATGAAGATATTTAAAGTTG+AGG + chr8_3:29992187-29992206 Msa1285450:CDS 20.0%
!! TTAAGTTATTTATGAAAGCA+TGG - chr8_3:29992256-29992275 None:intergenic 20.0%
!! AATCAGACAAATTATTGAAT+TGG + chr8_3:29992886-29992905 Msa1285450:intron 20.0%
!!! ATTATCAAGTTTTGCTAATA+AGG + chr8_3:29993123-29993142 Msa1285450:intron 20.0%
!!! TATCAAAACTTTTCTTAACA+TGG + chr8_3:29993827-29993846 Msa1285450:exon 20.0%
!! TAAATAAAACACAAATGTTG+TGG - chr8_3:29994150-29994169 None:intergenic 20.0%
!! TGTTGTAAATAATAATCAAG+TGG - chr8_3:29994177-29994196 None:intergenic 20.0%
! ACTTTAAATATCTTCATAGG+TGG - chr8_3:29992185-29992204 None:intergenic 25.0%
! GACATAAATATTTGTGAGAT+TGG - chr8_3:29992371-29992390 None:intergenic 25.0%
!!! AAATTCCTATAGCTTTGTTT+TGG + chr8_3:29992510-29992529 Msa1285450:intron 25.0%
!! AAAAATACATTTTGAGCAAG+TGG - chr8_3:29993031-29993050 None:intergenic 25.0%
! GAAGAGAGATGTTAATTATT+AGG - chr8_3:29993334-29993353 None:intergenic 25.0%
!!! AAGAATTTATTTTCTTCTCC+AGG + chr8_3:29993465-29993484 Msa1285450:intron 25.0%
!! TTTTCTGTACTCTGATAATT+GGG - chr8_3:29993651-29993670 None:intergenic 25.0%
! TCTTAAACAGATATGCATAT+AGG - chr8_3:29993805-29993824 None:intergenic 25.0%
! GAAAAGAAAGCAAAATAGAA+TGG - chr8_3:29993928-29993947 None:intergenic 25.0%
! AGGCTAAAATCATAAACTAT+AGG - chr8_3:29994049-29994068 None:intergenic 25.0%
! TGCCGAAAATTATATTGTTT+AGG - chr8_3:29994086-29994105 None:intergenic 25.0%
! CATTTAGTAGAAAAGACTAA+TGG + chr8_3:29994743-29994762 Msa1285450:intron 25.0%
! CTCTATTAAATTAAAGTTGG+AGG - chr8_3:29994881-29994900 Msa1285460:CDS 25.0%
! ACGCTCTATTAAATTAAAGT+TGG - chr8_3:29994884-29994903 Msa1285460:CDS 25.0%
TTCTAATCTTTGCAGTTACA+AGG + chr8_3:29991580-29991599 Msa1285450:five_prime_UTR 30.0%
CAATTTGTGTAGAAAGAATC+AGG - chr8_3:29991836-29991855 None:intergenic 30.0%
CTGATTCTTTCTACACAAAT+TGG + chr8_3:29991834-29991853 Msa1285450:CDS 30.0%
ATTCTTTCTACACAAATTGG+AGG + chr8_3:29991837-29991856 Msa1285450:CDS 30.0%
! GTTAAAATCTGGAAAAGTGT+TGG - chr8_3:29991896-29991915 None:intergenic 30.0%
AACAGATCATAGACTCAAAT+TGG - chr8_3:29992115-29992134 None:intergenic 30.0%
ATTTGAGTCTATGATCTGTT+TGG + chr8_3:29992115-29992134 Msa1285450:CDS 30.0%
! GAAATTGATTCAACCATGAA+TGG - chr8_3:29992572-29992591 None:intergenic 30.0%
TGATCATGCAACAAATCATA+CGG + chr8_3:29992592-29992611 Msa1285450:CDS 30.0%
CAAATCATACGGTACATAAA+CGG + chr8_3:29992603-29992622 Msa1285450:CDS 30.0%
AGTACTTGAATGAATGTTCA+TGG + chr8_3:29992781-29992800 Msa1285450:intron 30.0%
!! TGTGCTTTTTACACAACTAT+AGG - chr8_3:29992813-29992832 None:intergenic 30.0%
! GTTGTGTAAAAAGCACAAAA+TGG + chr8_3:29992816-29992835 Msa1285450:intron 30.0%
!! AACATGTTTTGTTCCAGATA+TGG + chr8_3:29992914-29992933 Msa1285450:intron 30.0%
TTATATTCTTGGACTTCATC+TGG - chr8_3:29992944-29992963 None:intergenic 30.0%
! ATAAGTTGGCGTTAAAAACA+TGG + chr8_3:29992978-29992997 Msa1285450:CDS 30.0%
! TAAGTTGGCGTTAAAAACAT+GGG + chr8_3:29992979-29992998 Msa1285450:CDS 30.0%
TAACTTACCACAAATGTGTT+GGG - chr8_3:29993066-29993085 None:intergenic 30.0%
AAACATTCACCAAATCATGT+GGG - chr8_3:29993209-29993228 None:intergenic 30.0%
AATGAATCATACCCAATACA+AGG + chr8_3:29993527-29993546 Msa1285450:CDS 30.0%
AATATGACGAACCTTGTATT+GGG - chr8_3:29993541-29993560 None:intergenic 30.0%
AATACAAGGTTCGTCATATT+GGG + chr8_3:29993541-29993560 Msa1285450:CDS 30.0%
TAATTGGGTGATGTTCAATA+GGG - chr8_3:29993636-29993655 None:intergenic 30.0%
ATAATTGGGTGATGTTCAAT+AGG - chr8_3:29993637-29993656 None:intergenic 30.0%
! CTTTTCTGTACTCTGATAAT+TGG - chr8_3:29993652-29993671 None:intergenic 30.0%
! TGCAATCAGCAAAAGATTTT+GGG - chr8_3:29993739-29993758 None:intergenic 30.0%
! ATGCAATCAGCAAAAGATTT+TGG - chr8_3:29993740-29993759 None:intergenic 30.0%
TATACAATCCAAAAGGCAAA+AGG - chr8_3:29993956-29993975 None:intergenic 30.0%
GTTCTGTTATACAATCCAAA+AGG - chr8_3:29993963-29993982 None:intergenic 30.0%
GAGATACAAACTGTAAACAA+AGG + chr8_3:29994006-29994025 Msa1285450:three_prime_UTR 30.0%
AGTATGAACAACTAGCAATA+TGG - chr8_3:29994324-29994343 Msa1285460:CDS 30.0%
!!! CCATTACTGTCATTTTTCAT+GGG - chr8_3:29994397-29994416 Msa1285460:intron 30.0%
!!! TCCATTACTGTCATTTTTCA+TGG - chr8_3:29994398-29994417 Msa1285460:intron 30.0%
CATTGATATGAGATGGTTGA+AGG - chr8_3:29991496-29991515 None:intergenic 35.0%
TTGGGAAAATTGATTGGATC+CGG - chr8_3:29991545-29991564 None:intergenic 35.0%
TGCAATGTATTTGAGGGAAA+TGG + chr8_3:29991723-29991742 Msa1285450:five_prime_UTR 35.0%
GCAATGTATTTGAGGGAAAT+GGG + chr8_3:29991724-29991743 Msa1285450:five_prime_UTR 35.0%
TATTTGAGGGAAATGGGTAT+GGG + chr8_3:29991730-29991749 Msa1285450:five_prime_UTR 35.0%
CACTTCTTCATAAAGAGGAT+AGG - chr8_3:29991765-29991784 None:intergenic 35.0%
CAACTCACTTCTTCATAAAG+AGG - chr8_3:29991770-29991789 None:intergenic 35.0%
ATCAAGGCGAAGTTAAAATC+TGG - chr8_3:29991907-29991926 None:intergenic 35.0%
! CTGAAGTCAAGAAAAGCATA+AGG - chr8_3:29992017-29992036 None:intergenic 35.0%
! CTTATGCTTTTCTTGACTTC+AGG + chr8_3:29992015-29992034 Msa1285450:intron 35.0%
AACATGTGCAACAACTTCAA+AGG - chr8_3:29992047-29992066 None:intergenic 35.0%
ATTGGAGACTCATTACAAAG+AGG + chr8_3:29992089-29992108 Msa1285450:CDS 35.0%
GTACAATCTGTAATTCCTGA+AGG + chr8_3:29992137-29992156 Msa1285450:CDS 35.0%
TCTTCATAGGTGGAATTCTT+TGG - chr8_3:29992175-29992194 None:intergenic 35.0%
!! ATGCATCGATTGAATACTAC+TGG + chr8_3:29992543-29992562 Msa1285450:CDS 35.0%
!! TGCATCGATTGAATACTACT+GGG + chr8_3:29992544-29992563 Msa1285450:CDS 35.0%
GTGAGTATATCTATAGACCT+TGG + chr8_3:29992736-29992755 Msa1285450:intron 35.0%
CGTTAAAAACATGGGCAAAT+TGG + chr8_3:29992987-29993006 Msa1285450:CDS 35.0%
! ATACATTTTGAGCAAGTGGT+TGG - chr8_3:29993027-29993046 None:intergenic 35.0%
AACTTACCACAAATGTGTTG+GGG - chr8_3:29993065-29993084 None:intergenic 35.0%
GTAACTTACCACAAATGTGT+TGG - chr8_3:29993067-29993086 None:intergenic 35.0%
GCAGAGTCACTCTTTAAATT+TGG - chr8_3:29993169-29993188 None:intergenic 35.0%
CAAACATTCACCAAATCATG+TGG - chr8_3:29993210-29993229 None:intergenic 35.0%
CAATATGACGAACCTTGTAT+TGG - chr8_3:29993542-29993561 None:intergenic 35.0%
CAATACAAGGTTCGTCATAT+TGG + chr8_3:29993540-29993559 Msa1285450:CDS 35.0%
ATACAAGGTTCGTCATATTG+GGG + chr8_3:29993542-29993561 Msa1285450:CDS 35.0%
TAGCTCTCTGTTCTTTAGTT+AGG - chr8_3:29993715-29993734 None:intergenic 35.0%
GGATTTCATTCCATGTATCT+GGG - chr8_3:29993784-29993803 None:intergenic 35.0%
AGGATTTCATTCCATGTATC+TGG - chr8_3:29993785-29993804 None:intergenic 35.0%
ACAAGCATCATTCAGATTGA+TGG + chr8_3:29993872-29993891 Msa1285450:three_prime_UTR 35.0%
!! TGATTTTAGCCTGTTCTTCA+TGG + chr8_3:29994057-29994076 Msa1285450:three_prime_UTR 35.0%
! TTTAGGTTTCCATGAAGAAC+AGG - chr8_3:29994069-29994088 None:intergenic 35.0%
CAAATGTACATGTCAAAGGT+GGG - chr8_3:29994115-29994134 None:intergenic 35.0%
GTTGCAAATGTACATGTCAA+AGG - chr8_3:29994119-29994138 None:intergenic 35.0%
GAATTAAATCGTCTGAGAGT+TGG + chr8_3:29994259-29994278 Msa1285450:three_prime_UTR 35.0%
GAACAACTAGCAATATGGTT+TGG - chr8_3:29994319-29994338 Msa1285460:CDS 35.0%
CCCATGAAAAATGACAGTAA+TGG + chr8_3:29994394-29994413 Msa1285450:intron 35.0%
CCAAAAGCTTAGATCGTATA+AGG - chr8_3:29994773-29994792 Msa1285460:CDS 35.0%
! CCTTATACGATCTAAGCTTT+TGG + chr8_3:29994770-29994789 Msa1285450:intron 35.0%
CGGTATATTTGTGAATGATC+GGG - chr8_3:29994821-29994840 Msa1285460:CDS 35.0%
GCGGTATATTTGTGAATGAT+CGG - chr8_3:29994822-29994841 Msa1285460:CDS 35.0%
!! GAGACAGCATTGATATGAGA+TGG - chr8_3:29991503-29991522 None:intergenic 40.0%
!! AGCCATGTTTTTCAGCTCTA+CGG + chr8_3:29991633-29991652 Msa1285450:five_prime_UTR 40.0%
GAGGGTTGCAATGTATTTGA+GGG + chr8_3:29991717-29991736 Msa1285450:five_prime_UTR 40.0%
GTATTTGAGGGAAATGGGTA+TGG + chr8_3:29991729-29991748 Msa1285450:five_prime_UTR 40.0%
AGACCACTTGCATGAAGAAT+GGG + chr8_3:29991807-29991826 Msa1285450:exon 40.0%
CTTTCTACACAAATTGGAGG+TGG + chr8_3:29991840-29991859 Msa1285450:CDS 40.0%
ACTTCGCCTTGATTACTCTT+AGG + chr8_3:29991914-29991933 Msa1285450:intron 40.0%
CGGAAACCTAAGAGTAATCA+AGG - chr8_3:29991923-29991942 None:intergenic 40.0%
TGAAGTTGTTGCACATGTTG+AGG + chr8_3:29992048-29992067 Msa1285450:CDS 40.0%
CATAGGTGGAATTCTTTGGA+GGG - chr8_3:29992171-29992190 None:intergenic 40.0%
TCATAGGTGGAATTCTTTGG+AGG - chr8_3:29992172-29992191 None:intergenic 40.0%
!!! TCCTATAGCTTTGTTTTGGC+AGG + chr8_3:29992514-29992533 Msa1285450:intron 40.0%
TCCTGCCAAAACAAAGCTAT+AGG - chr8_3:29992518-29992537 None:intergenic 40.0%
TCATACGGTACATAAACGGA+TGG + chr8_3:29992607-29992626 Msa1285450:CDS 40.0%
CTTGACTCAATAGCTAAGCA+TGG + chr8_3:29992635-29992654 Msa1285450:CDS 40.0%
TAGCTAAGCATGGCAAACAT+TGG + chr8_3:29992645-29992664 Msa1285450:CDS 40.0%
!! CTCACGTAGCATTGATGAAA+GGG - chr8_3:29992721-29992740 None:intergenic 40.0%
!! ACTCACGTAGCATTGATGAA+AGG - chr8_3:29992722-29992741 None:intergenic 40.0%
GTATATCTATAGACCTTGGC+TGG + chr8_3:29992740-29992759 Msa1285450:intron 40.0%
!! CAAGTACTGAACTATGCTCA+CGG - chr8_3:29992769-29992788 None:intergenic 40.0%
TTCATCTGGTGATCCATATC+TGG - chr8_3:29992930-29992949 None:intergenic 40.0%
CTGTCGTGACGTTATATTCT+TGG - chr8_3:29992955-29992974 None:intergenic 40.0%
AAAGTGAAGCCCACATGATT+TGG + chr8_3:29993197-29993216 Msa1285450:intron 40.0%
! TTATTTTCTTCTCCAGGAGC+TGG + chr8_3:29993471-29993490 Msa1285450:intron 40.0%
! TATTTTCTTCTCCAGGAGCT+GGG + chr8_3:29993472-29993491 Msa1285450:intron 40.0%
!! CTTTTGCTGATTGCATTCAC+TGG + chr8_3:29993744-29993763 Msa1285450:CDS 40.0%
AGCATCATTCAGATTGATGG+TGG + chr8_3:29993875-29993894 Msa1285450:three_prime_UTR 40.0%
! TTCCAACACCTTTTGCCTTT+TGG + chr8_3:29993945-29993964 Msa1285450:three_prime_UTR 40.0%
! ATCCAAAAGGCAAAAGGTGT+TGG - chr8_3:29993950-29993969 None:intergenic 40.0%
GCAAATGTACATGTCAAAGG+TGG - chr8_3:29994116-29994135 None:intergenic 40.0%
TTAAATCGTCTGAGAGTTGG+TGG + chr8_3:29994262-29994281 Msa1285450:three_prime_UTR 40.0%
TTCGAAGAAGAGGTTAGCAA+AGG - chr8_3:29994347-29994366 Msa1285460:CDS 40.0%
ATGCATTCTCTTCGAAGAAG+AGG - chr8_3:29994357-29994376 Msa1285460:CDS 40.0%
TCAAGGAAACGATGAAGATG+TGG + chr8_3:29994501-29994520 Msa1285450:intron 40.0%
GATACAAAGCGTGAAGATAG+CGG - chr8_3:29994841-29994860 Msa1285460:CDS 40.0%
TCAAGTCTTCGATTGATCGC+CGG + chr8_3:29991523-29991542 Msa1285450:five_prime_UTR 45.0%
GCTTGCTCTTCAAGCTATCT+AGG - chr8_3:29991611-29991630 None:intergenic 45.0%
TGCCGTAGAGCTGAAAAACA+TGG - chr8_3:29991638-29991657 None:intergenic 45.0%
TGGTGGAAGGAATCGATTAC+CGG + chr8_3:29991665-29991684 Msa1285450:five_prime_UTR 45.0%
! ATTACCGGTTGTTGTGTTGC+CGG + chr8_3:29991680-29991699 Msa1285450:five_prime_UTR 45.0%
GGAGGGTTGCAATGTATTTG+AGG + chr8_3:29991716-29991735 Msa1285450:five_prime_UTR 45.0%
AGAAGTGAGTTGCCCTTACT+TGG + chr8_3:29991778-29991797 Msa1285450:five_prime_UTR 45.0%
TGGTCTGCTTGACCAAGTAA+GGG - chr8_3:29991793-29991812 None:intergenic 45.0%
CAGACCACTTGCATGAAGAA+TGG + chr8_3:29991806-29991825 Msa1285450:exon 45.0%
TCTCCCATTCTTCATGCAAG+TGG - chr8_3:29991813-29991832 None:intergenic 45.0%
!! GGAAAAGTGTTGGCTACCTT+GGG - chr8_3:29991886-29991905 None:intergenic 45.0%
!! TGGAAAAGTGTTGGCTACCT+TGG - chr8_3:29991887-29991906 None:intergenic 45.0%
TGGAGGGATTTCTTTCCTTC+AGG - chr8_3:29992155-29992174 None:intergenic 45.0%
ATACTACTGGGCTCCATTCA+TGG + chr8_3:29992556-29992575 Msa1285450:CDS 45.0%
AAGCATGGCAAACATTGGCA+AGG + chr8_3:29992650-29992669 Msa1285450:CDS 45.0%
! TTGGCAAGGAGTGGACATTT+TGG + chr8_3:29992664-29992683 Msa1285450:CDS 45.0%
TGGTGTTCGAGAGCTATGTT+TGG + chr8_3:29992684-29992703 Msa1285450:CDS 45.0%
TGTTCGAGAGCTATGTTTGG+TGG + chr8_3:29992687-29992706 Msa1285450:CDS 45.0%
CGTCACGACAGCATATAAGT+TGG + chr8_3:29992964-29992983 Msa1285450:CDS 45.0%
GTATGTCCCCAACACATTTG+TGG + chr8_3:29993056-29993075 Msa1285450:intron 45.0%
! AAGCAGTTTTCGTCACTTCC+TGG - chr8_3:29993506-29993525 None:intergenic 45.0%
GATGCTCACACCTCAGTTTA+TGG + chr8_3:29993671-29993690 Msa1285450:CDS 45.0%
TCAGTTTATGGCGAGCGAAA+GGG + chr8_3:29993683-29993702 Msa1285450:CDS 45.0%
TGCATTCACTGGTGTTTACC+TGG + chr8_3:29993755-29993774 Msa1285450:CDS 45.0%
GCATTCACTGGTGTTTACCT+GGG + chr8_3:29993756-29993775 Msa1285450:CDS 45.0%
CATTCACTGGTGTTTACCTG+GGG + chr8_3:29993757-29993776 Msa1285450:CDS 45.0%
!! GCAAGCTTCTGAAACTGAGT+GGG - chr8_3:29994203-29994222 Msa1285460:CDS 45.0%
!! AGCAAGCTTCTGAAACTGAG+TGG - chr8_3:29994204-29994223 Msa1285460:CDS 45.0%
CGTTGAGTTCACGTATGTTC+TGG + chr8_3:29994237-29994256 Msa1285450:three_prime_UTR 45.0%
!! GTTGGTGGTAAATCTTGACC+TGG + chr8_3:29994277-29994296 Msa1285450:three_prime_UTR 45.0%
TCTTCGAAGAGAATGCATCC+TGG + chr8_3:29994359-29994378 Msa1285450:three_prime_UTR 45.0%
GTGGTCTGCTTGACCAAGTA+AGG - chr8_3:29991794-29991813 None:intergenic 50.0%
!! AAAGTGTTGGCTACCTTGGG+AGG - chr8_3:29991883-29991902 None:intergenic 50.0%
TGGCAAACATTGGCAAGGAG+TGG + chr8_3:29992655-29992674 Msa1285450:CDS 50.0%
CTTTCGCTCGCCATAAACTG+AGG - chr8_3:29993684-29993703 None:intergenic 50.0%
CTCAGTTTATGGCGAGCGAA+AGG + chr8_3:29993682-29993701 Msa1285450:CDS 50.0%
CATGGGACAGAAAAGACTCC+AGG - chr8_3:29994380-29994399 Msa1285460:intron 50.0%
CAGCTCTACGGCATGTCATC+TGG + chr8_3:29991645-29991664 Msa1285450:five_prime_UTR 55.0%
CTCTACGGCATGTCATCTGG+TGG + chr8_3:29991648-29991667 Msa1285450:five_prime_UTR 55.0%
ACGGCATGTCATCTGGTGGA+AGG + chr8_3:29991652-29991671 Msa1285450:five_prime_UTR 55.0%
TCCTCCGGCAACACAACAAC+CGG - chr8_3:29991687-29991706 None:intergenic 55.0%
AATTGGAGGTGGCAGCCTCA+TGG + chr8_3:29991851-29991870 Msa1285450:CDS 55.0%
CTTGGGAGGTTGCATCCATG+AGG - chr8_3:29991869-29991888 None:intergenic 55.0%
CTCATGGATGCAACCTCCCA+AGG + chr8_3:29991867-29991886 Msa1285450:CDS 55.0%
TCTTCTCCAGGAGCTGGGAA+TGG + chr8_3:29993477-29993496 Msa1285450:intron 55.0%
GGTTCGTCATATTGGGGCAC+AGG + chr8_3:29993548-29993567 Msa1285450:CDS 55.0%
TACCTGGGGTCCCAGATACA+TGG + chr8_3:29993771-29993790 Msa1285450:CDS 55.0%
! TTCCATGTATCTGGGACCCC+AGG - chr8_3:29993776-29993795 None:intergenic 55.0%
GGAGATGCTGATATCGAGCC+AGG - chr8_3:29994298-29994317 Msa1285460:CDS 55.0%
ACCGGTTGTTGTGTTGCCGG+AGG + chr8_3:29991683-29991702 Msa1285450:five_prime_UTR 60.0%
GTTGCCGGAGGATCGCATTG+AGG + chr8_3:29991695-29991714 Msa1285450:five_prime_UTR 60.0%
CCCTCCTCAATGCGATCCTC+CGG - chr8_3:29991702-29991721 None:intergenic 60.0%
CCGGAGGATCGCATTGAGGA+GGG + chr8_3:29991699-29991718 Msa1285450:five_prime_UTR 60.0%
ATGCTCACGGAGACCAGCCA+AGG - chr8_3:29992756-29992775 None:intergenic 60.0%
TGGCTTCCATTCCCAGCTCC+TGG - chr8_3:29993486-29993505 None:intergenic 60.0%
AGGAGCTGGGAATGGAAGCC+AGG + chr8_3:29993485-29993504 Msa1285450:intron 60.0%
GCCGGAGGATCGCATTGAGG+AGG + chr8_3:29991698-29991717 Msa1285450:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 29991463 29994908 29991463 ID=Msa1285450;Name=Msa1285450
chr8_3 mRNA 29991463 29994908 29991463 ID=Msa1285450-mRNA-1;Parent=Msa1285450;Name=Msa1285450-mRNA-1;_AED=0.08;_eAED=0.08;_QI=355|0.8|0.83|1|0.6|0.5|6|682|314
chr8_3 exon 29991463 29991888 29991463 ID=Msa1285450-mRNA-1:exon:9836;Parent=Msa1285450-mRNA-1
chr8_3 exon 29992037 29992208 29992037 ID=Msa1285450-mRNA-1:exon:9837;Parent=Msa1285450-mRNA-1
chr8_3 exon 29992536 29992735 29992536 ID=Msa1285450-mRNA-1:exon:9838;Parent=Msa1285450-mRNA-1
chr8_3 exon 29992931 29993074 29992931 ID=Msa1285450-mRNA-1:exon:9839;Parent=Msa1285450-mRNA-1
chr8_3 exon 29993487 29994398 29993487 ID=Msa1285450-mRNA-1:exon:9840;Parent=Msa1285450-mRNA-1
chr8_3 exon 29994781 29994908 29994781 ID=Msa1285450-mRNA-1:exon:9841;Parent=Msa1285450-mRNA-1
chr8_3 five_prime_UTR 29991463 29991817 29991463 ID=Msa1285450-mRNA-1:five_prime_utr;Parent=Msa1285450-mRNA-1
chr8_3 CDS 29991818 29991888 29991818 ID=Msa1285450-mRNA-1:cds;Parent=Msa1285450-mRNA-1
chr8_3 CDS 29992037 29992208 29992037 ID=Msa1285450-mRNA-1:cds;Parent=Msa1285450-mRNA-1
chr8_3 CDS 29992536 29992735 29992536 ID=Msa1285450-mRNA-1:cds;Parent=Msa1285450-mRNA-1
chr8_3 CDS 29992931 29993074 29992931 ID=Msa1285450-mRNA-1:cds;Parent=Msa1285450-mRNA-1
chr8_3 CDS 29993487 29993844 29993487 ID=Msa1285450-mRNA-1:cds;Parent=Msa1285450-mRNA-1
chr8_3 three_prime_UTR 29993845 29994398 29993845 ID=Msa1285450-mRNA-1:three_prime_utr;Parent=Msa1285450-mRNA-1
chr8_3 three_prime_UTR 29994781 29994908 29994781 ID=Msa1285450-mRNA-1:three_prime_utr;Parent=Msa1285450-mRNA-1
Gene Sequence

>Msa1285450

ATGAAGAATGGGAGACCTGATTCTTTCTACACAAATTGGAGGTGGCAGCCTCATGGATGCAACCTCCCAAGGTTTGATCCTTTGAAGTTGTTGCACATGTTGAGGAACAAAAGAATGATGTTTATTGGAGACTCATTACAAAGAGGCCAATTTGAGTCTATGATCTGTTTGGTACAATCTGTAATTCCTGAAGGAAAGAAATCCCTCCAAAGAATTCCACCTATGAAGATATTTAAAGTTGAGGAGTTTAATGCATCGATTGAATACTACTGGGCTCCATTCATGGTTGAATCAATTTCTGATCATGCAACAAATCATACGGTACATAAACGGATGGTGATGCTTGACTCAATAGCTAAGCATGGCAAACATTGGCAAGGAGTGGACATTTTGGTGTTCGAGAGCTATGTTTGGTGGATGCACAGCCCTTTCATCAATGCTACATATGGATCACCAGATGAAGTCCAAGAATATAACGTCACGACAGCATATAAGTTGGCGTTAAAAACATGGGCAAATTGGTTAGAATCAAACATCCAACCACTTGCTCAAAATGTATTTTTCATGAGTATGTCCCCAACACATTTGAGCTGGGAATGGAAGCCAGGAAGTGACGAAAACTGCTTCAATGAATCATACCCAATACAAGGTTCGTCATATTGGGGCACAGGCTCGAATCTTGAGATTATGAAGATATTACATGATTCACTACAAGAACTGAAAATAGATGTCACCCTATTGAACATCACCCAATTATCAGAGTACAGAAAAGATGCTCACACCTCAGTTTATGGCGAGCGAAAGGGCAAGCTCCTAACTAAAGAACAGAGAGCTAACCCAAAATCTTTTGCTGATTGCATTCACTGGTGTTTACCTGGGGTCCCAGATACATGGAATGAAATCCTATATGCATATCTGTTTAAGAACTATCAAAACTTTTCTTAA
Protein sequence

>Msa1285450

MKNGRPDSFYTNWRWQPHGCNLPRFDPLKLLHMLRNKRMMFIGDSLQRGQFESMICLVQSVIPEGKKSLQRIPPMKIFKVEEFNASIEYYWAPFMVESISDHATNHTVHKRMVMLDSIAKHGKHWQGVDILVFESYVWWMHSPFINATYGSPDEVQEYNVTTAYKLALKTWANWLESNIQPLAQNVFFMSMSPTHLSWEWKPGSDENCFNESYPIQGSSYWGTGSNLEIMKILHDSLQELKIDVTLLNITQLSEYRKDAHTSVYGERKGKLLTKEQRANPKSFADCIHWCLPGVPDTWNEILYAYLFKNYQNFS*