AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1297400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297400 MtrunA17_Chr4g0054521 94.030 67 3 1 56 121 864 930 2.76e-35 128
Msa1297400 MtrunA17_Chr5g0400681 91.045 67 5 1 56 121 867 933 1.77e-33 122
Msa1297400 MtrunA17_Chr3g0095081 64.179 67 22 2 56 120 902 968 4.29e-22 90.5
Msa1297400 MtrunA17_Chr2g0277081 63.077 65 23 1 56 119 862 926 9.97e-22 89.4
Msa1297400 MtrunA17_Chr5g0442151 66.667 69 20 3 56 121 858 926 1.21e-21 89.4
Msa1297400 MtrunA17_Chr4g0035464 65.714 70 18 2 57 120 860 929 3.21e-21 87.8
Msa1297400 MtrunA17_Chr7g0262141 60.345 58 22 1 57 113 889 946 3.56e-17 76.3
Msa1297400 MtrunA17_Chr3g0140871 49.275 69 34 1 57 124 864 932 7.88e-16 72.4
Msa1297400 MtrunA17_Chr4g0017251 53.125 64 29 1 56 118 850 913 7.68e-15 69.7
Msa1297400 MtrunA17_Chr1g0183231 55.000 60 25 2 57 115 880 938 8.90e-15 69.7
Msa1297400 MtrunA17_Chr4g0058761 53.226 62 28 1 59 119 951 1012 1.21e-14 69.3
Msa1297400 MtrunA17_Chr4g0046511 60.938 64 22 3 56 118 872 933 1.62e-14 68.9
Msa1297400 MtrunA17_Chr4g0018701 52.632 57 26 1 57 112 897 953 1.50e-13 65.9
Msa1297400 MtrunA17_Chr2g0280191 52.459 61 28 1 56 115 866 926 1.90e-13 65.9
Msa1297400 MtrunA17_Chr1g0190411 53.333 60 26 2 57 115 886 944 2.06e-13 65.5
Msa1297400 MtrunA17_Chr2g0282831 58.730 63 23 3 56 117 844 904 2.11e-13 65.5
Msa1297400 MtrunA17_Chr1g0201241 46.970 66 32 1 57 119 898 963 3.34e-13 65.1
Msa1297400 MtrunA17_Chr2g0312651 50.000 60 30 0 56 115 984 1043 6.24e-13 64.3
Msa1297400 MtrunA17_Chr5g0402071 44.262 61 31 2 61 119 346 405 1.04e-11 60.8
Msa1297400 MtrunA17_Chr3g0140861 47.619 63 30 2 56 115 836 898 1.68e-11 60.1
Msa1297400 MtrunA17_Chr5g0419721 50.000 64 26 2 57 119 824 882 3.64e-11 59.3
Msa1297400 MtrunA17_Chr1g0169841 47.541 61 30 1 57 115 987 1047 4.42e-11 58.9
Msa1297400 MtrunA17_Chr2g0296591 48.684 76 34 4 57 128 1130 1204 5.50e-11 58.5
Msa1297400 MtrunA17_Chr4g0018801 47.273 55 28 1 59 112 670 724 6.91e-11 58.5
Msa1297400 MtrunA17_Chr5g0400701 56.522 46 19 1 57 101 883 928 8.15e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297400 AT5G65700.1 87.879 66 7 1 56 120 869 934 1.25e-31 118
Msa1297400 AT5G65700.2 87.879 66 7 1 56 120 869 934 1.25e-31 118
Msa1297400 AT3G49670.1 81.818 66 11 1 56 120 865 930 8.47e-29 110
Msa1297400 AT1G75820.1 63.380 71 20 2 56 120 866 936 4.29e-22 90.9
Msa1297400 AT4G20270.1 61.194 67 24 2 56 120 886 952 8.71e-22 90.1
Msa1297400 AT4G28650.1 56.667 60 25 1 57 115 879 938 8.15e-16 72.8
Msa1297400 AT1G09970.2 51.667 60 28 1 57 115 857 916 4.85e-14 67.8
Msa1297400 AT1G09970.1 51.667 60 28 1 57 115 856 915 5.00e-14 67.8
Msa1297400 AT1G08590.1 48.333 60 30 1 57 115 902 961 6.52e-14 67.4
Msa1297400 AT5G49660.1 50.820 61 29 1 56 115 837 897 3.05e-13 65.5
Msa1297400 AT1G34110.2 48.438 64 32 1 57 119 951 1014 5.29e-13 64.7
Msa1297400 AT1G34110.1 48.438 64 32 1 57 119 951 1014 5.40e-13 64.7
Msa1297400 AT5G61480.1 47.541 61 31 1 56 115 898 958 1.61e-12 63.5
Msa1297400 AT5G63930.2 50.000 60 30 0 56 115 916 975 3.23e-12 62.8
Msa1297400 AT5G63930.1 50.000 60 30 0 56 115 980 1039 3.64e-12 62.4
Msa1297400 AT4G28490.1 54.098 61 25 3 56 115 870 928 9.71e-12 61.2
Msa1297400 AT2G33170.2 43.284 67 38 0 56 122 1000 1066 1.42e-11 60.8
Msa1297400 AT2G33170.1 43.284 67 38 0 56 122 1000 1066 1.42e-11 60.8
Msa1297400 AT1G17230.2 47.458 59 31 0 57 115 975 1033 1.54e-11 60.8
Msa1297400 AT1G17230.1 47.458 59 31 0 57 115 975 1033 1.65e-11 60.5
Msa1297400 AT1G28440.1 50.000 64 29 3 56 118 865 926 1.65e-11 60.5
Msa1297400 AT3G19700.1 65.116 43 14 1 56 97 865 907 1.80e-11 60.5
Msa1297400 AT3G24240.1 48.387 62 27 2 57 117 969 1026 3.35e-11 59.7
Msa1297400 AT5G48940.1 45.312 64 29 2 57 119 970 1028 7.06e-11 58.9

Find 64 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCAGTTCCACTTGATTT+GGG 0.156263 8_3:+57699330 Msa1297400:CDS
AGAAAACCAGTTGGAGAATT+TGG 0.201190 8_3:+57699225 Msa1297400:CDS
TCCTTCAGTTCCACTTGATT+TGG 0.221091 8_3:+57699329 Msa1297400:CDS
GGAAGAGGATGAGTGGTAGA+AGG 0.272087 8_3:-57698817 None:intergenic
TTTCTTCGCCATTTGCATCT+TGG 0.292810 8_3:+57698944 Msa1297400:CDS
AGCTGTGCTGTGCTATTTAA+AGG 0.293216 8_3:-57698635 None:intergenic
CTTCAAATCCTCTCCTGTTT+CGG 0.326008 8_3:+57698907 Msa1297400:CDS
ATAAAAGAAAATACTATATC+TGG 0.329689 8_3:-57698283 None:intergenic
TGATTGGCTGGTGGTTCATT+CGG 0.332631 8_3:+57698588 Msa1297400:five_prime_UTR
GAAACTATATCGCAATTATA+AGG 0.346358 8_3:+57698761 Msa1297400:five_prime_UTR
GGTTGAATGAGAGGAGGAAG+AGG 0.371326 8_3:-57698832 None:intergenic
GATTGGCTGGTGGTTCATTC+GGG 0.373632 8_3:+57698589 Msa1297400:five_prime_UTR
GAAGAGGATGAGTGGTAGAA+GGG 0.378013 8_3:-57698816 None:intergenic
GAGAGATGGGTGACCGAAAC+AGG 0.379925 8_3:-57698920 None:intergenic
TTAACACGTATTTGTTCAAA+TGG 0.381958 8_3:+57698326 Msa1297400:five_prime_UTR
AAAATACTATATCTGGTAGA+AGG 0.420453 8_3:-57698276 None:intergenic
TGTGGGGTGTGGAAAGTACT+TGG 0.421647 8_3:-57698859 None:intergenic
CCATCACCAAATTCTCCAAC+TGG 0.439622 8_3:-57699231 None:intergenic
CTTCGCCATTTGCATCTTGG+AGG 0.464181 8_3:+57698947 Msa1297400:CDS
AAAAGCGACGTTTACAGCTT+CGG 0.468170 8_3:+57699174 Msa1297400:CDS
TGGTTCATTCGGGATAGTCT+CGG 0.481402 8_3:+57698599 Msa1297400:five_prime_UTR
GTGTGAAAGAGAGAGGGAAT+GGG 0.483412 8_3:-57698237 None:intergenic
CTTCAGTTCCACTTGATTTG+GGG 0.485130 8_3:+57699331 Msa1297400:CDS
CGAAACAGGAGAGGATTTGA+AGG 0.488102 8_3:-57698906 None:intergenic
TGAGTGGTATTCAGAGATGT+GGG 0.503238 8_3:-57698876 None:intergenic
AAGAGGATGAGTGGTAGAAG+GGG 0.513080 8_3:-57698815 None:intergenic
ATTTGAAGGAGAGGAGTGAG+TGG 0.514237 8_3:-57698892 None:intergenic
GTGAGTGGTATTCAGAGATG+TGG 0.523967 8_3:-57698877 None:intergenic
GGTTCATTCGGGATAGTCTC+GGG 0.527514 8_3:+57698600 Msa1297400:five_prime_UTR
CAGGAGAGGATTTGAAGGAG+AGG 0.528888 8_3:-57698901 None:intergenic
TGTGTGAAAGAGAGAGGGAA+TGG 0.529008 8_3:-57698238 None:intergenic
GTACTTCTAGAGCTTGTAGC+AGG 0.534823 8_3:+57699201 Msa1297400:CDS
CTTGGGTAAGGTTGAATGAG+AGG 0.535384 8_3:-57698841 None:intergenic
TCGCAATTATAAGGCTAAGC+TGG 0.538745 8_3:+57698770 Msa1297400:five_prime_UTR
AGTTACCTCCAAGATGCAAA+TGG 0.542647 8_3:-57698952 None:intergenic
GAGAGGAGGAAGAGGATGAG+TGG 0.543137 8_3:-57698824 None:intergenic
GCTGTGCTGTGCTATTTAAA+GGG 0.547042 8_3:-57698634 None:intergenic
TGGAGTAGACATTGTGCAAT+GGG 0.549400 8_3:+57699251 Msa1297400:CDS
CTGGCGGCAAAGCGGCGATG+AGG 0.549532 8_3:+57698789 Msa1297400:exon
AGTGGAACTGAAGGAAGTCT+AGG 0.552601 8_3:-57699321 None:intergenic
TGCTGTGCTATTTAAAGGGA+AGG 0.562386 8_3:-57698630 None:intergenic
CCAGTTGGAGAATTTGGTGA+TGG 0.564487 8_3:+57699231 Msa1297400:CDS
CCCAAATCAAGTGGAACTGA+AGG 0.566973 8_3:-57699330 None:intergenic
GTATTCAGAGATGTGGGGTG+TGG 0.567728 8_3:-57698870 None:intergenic
AACAAACACCCCAAATCAAG+TGG 0.567756 8_3:-57699339 None:intergenic
GTGTGGAAAGTACTTGGGTA+AGG 0.576259 8_3:-57698853 None:intergenic
GACTATCGATAGTTTCACAT+TGG 0.588550 8_3:+57698683 Msa1297400:five_prime_UTR
TTGTGTGTGTGAAAGAGAGA+GGG 0.591594 8_3:-57698243 None:intergenic
ATGGCGAAGAAAAGAGAGAT+GGG 0.595466 8_3:-57698933 None:intergenic
GTGGGGTGTGGAAAGTACTT+GGG 0.597175 8_3:-57698858 None:intergenic
AATGGCGAAGAAAAGAGAGA+TGG 0.599455 8_3:-57698934 None:intergenic
AAAATGACAGACTCAAACAA+AGG 0.599944 8_3:+57699279 Msa1297400:CDS
TTTGTGTGTGTGAAAGAGAG+AGG 0.600059 8_3:-57698244 None:intergenic
TGTGAAAGAGAGAGGGAATG+GGG 0.601573 8_3:-57698236 None:intergenic
GGGTAAGGTTGAATGAGAGG+AGG 0.606338 8_3:-57698838 None:intergenic
CGTGTTAAATGTATCCACAA+TGG 0.617828 8_3:-57698311 None:intergenic
ATGGAGTAGACATTGTGCAA+TGG 0.636956 8_3:+57699250 Msa1297400:CDS
CAATTATAAGGCTAAGCTGG+CGG 0.638574 8_3:+57698773 Msa1297400:five_prime_UTR
ATGGGTGACCGAAACAGGAG+AGG 0.645390 8_3:-57698915 None:intergenic
AGGCTAAGCTGGCGGCAAAG+CGG 0.651684 8_3:+57698781 Msa1297400:five_prime_UTR
TAGTTTCGTGACGAAGACGA+TGG 0.666610 8_3:-57698745 None:intergenic
GTAGCAGGAAGAAAACCAGT+TGG 0.667156 8_3:+57699216 Msa1297400:CDS
GAGTGGTATTCAGAGATGTG+GGG 0.694116 8_3:-57698875 None:intergenic
CGACGTTTACAGCTTCGGTG+TGG 0.695824 8_3:+57699179 Msa1297400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAGAAAATACTATATC+TGG - chr8_3:57698286-57698305 None:intergenic 15.0%
!! CAGTAAATTTATTTATAACA+CGG + chr8_3:57698510-57698529 Msa1297400:intron 15.0%
!! ATAAAAGAAAATACTATATC+TGG - chr8_3:57698286-57698305 None:intergenic 15.0%
!! CAGTAAATTTATTTATAACA+CGG + chr8_3:57698510-57698529 Msa1297400:intron 15.0%
!! TCATTCAATGAAAATTAAGA+AGG - chr8_3:57698434-57698453 None:intergenic 20.0%
!!! TTGAATTATTTTGTAAAAGC+AGG + chr8_3:57698561-57698580 Msa1297400:intron 20.0%
!! ATCAAGATTCAAACTATTAA+GGG - chr8_3:57699002-57699021 None:intergenic 20.0%
!! TTAATAGTTTGAATCTTGAT+CGG + chr8_3:57699002-57699021 Msa1297400:intron 20.0%
!! TCATCATAAACAAAAAGAAT+CGG - chr8_3:57699100-57699119 None:intergenic 20.0%
!! TCATTCAATGAAAATTAAGA+AGG - chr8_3:57698434-57698453 None:intergenic 20.0%
!!! TTGAATTATTTTGTAAAAGC+AGG + chr8_3:57698561-57698580 Msa1297400:intron 20.0%
!! ATCAAGATTCAAACTATTAA+GGG - chr8_3:57699002-57699021 None:intergenic 20.0%
!! TTAATAGTTTGAATCTTGAT+CGG + chr8_3:57699002-57699021 Msa1297400:intron 20.0%
!! TCATCATAAACAAAAAGAAT+CGG - chr8_3:57699100-57699119 None:intergenic 20.0%
! AAAATACTATATCTGGTAGA+AGG - chr8_3:57698279-57698298 None:intergenic 25.0%
!! TTCTTTTATACAAACCATTG+TGG + chr8_3:57698297-57698316 Msa1297400:five_prime_UTR 25.0%
! TTAACACGTATTTGTTCAAA+TGG + chr8_3:57698326-57698345 Msa1297400:five_prime_UTR 25.0%
! AAAAAAAAGCAGAAATAGCA+TGG - chr8_3:57698410-57698429 None:intergenic 25.0%
! GAAACTATATCGCAATTATA+AGG + chr8_3:57698761-57698780 Msa1297400:five_prime_UTR 25.0%
! GATCAAGATTCAAACTATTA+AGG - chr8_3:57699003-57699022 None:intergenic 25.0%
! CTAAAATACCTAGTTTGAAA+TGG + chr8_3:57699063-57699082 Msa1297400:intron 25.0%
! AAAATACTATATCTGGTAGA+AGG - chr8_3:57698279-57698298 None:intergenic 25.0%
!! TTCTTTTATACAAACCATTG+TGG + chr8_3:57698297-57698316 Msa1297400:five_prime_UTR 25.0%
! TTAACACGTATTTGTTCAAA+TGG + chr8_3:57698326-57698345 Msa1297400:five_prime_UTR 25.0%
! AAAAAAAAGCAGAAATAGCA+TGG - chr8_3:57698410-57698429 None:intergenic 25.0%
! GAAACTATATCGCAATTATA+AGG + chr8_3:57698761-57698780 Msa1297400:five_prime_UTR 25.0%
! GATCAAGATTCAAACTATTA+AGG - chr8_3:57699003-57699022 None:intergenic 25.0%
! CTAAAATACCTAGTTTGAAA+TGG + chr8_3:57699063-57699082 Msa1297400:intron 25.0%
ACGTATTTGTTCAAATGGTA+AGG + chr8_3:57698331-57698350 Msa1297400:intron 30.0%
AATGTGCATGTGTATAAGAA+TGG - chr8_3:57698476-57698495 None:intergenic 30.0%
ATAACACGGCAATTGATATT+TGG + chr8_3:57698524-57698543 Msa1297400:intron 30.0%
!! TGTAAAAGCAGGATTTTGAT+TGG + chr8_3:57698572-57698591 Msa1297400:intron 30.0%
AAAATGACAGACTCAAACAA+AGG + chr8_3:57699279-57699298 Msa1297400:CDS 30.0%
ACGTATTTGTTCAAATGGTA+AGG + chr8_3:57698331-57698350 Msa1297400:intron 30.0%
AATGTGCATGTGTATAAGAA+TGG - chr8_3:57698476-57698495 None:intergenic 30.0%
ATAACACGGCAATTGATATT+TGG + chr8_3:57698524-57698543 Msa1297400:intron 30.0%
!! TGTAAAAGCAGGATTTTGAT+TGG + chr8_3:57698572-57698591 Msa1297400:intron 30.0%
AAAATGACAGACTCAAACAA+AGG + chr8_3:57699279-57699298 Msa1297400:CDS 30.0%
CGTGTTAAATGTATCCACAA+TGG - chr8_3:57698314-57698333 None:intergenic 35.0%
AAAAGCAGAAATAGCATGGT+GGG - chr8_3:57698406-57698425 None:intergenic 35.0%
AAAAAGCAGAAATAGCATGG+TGG - chr8_3:57698407-57698426 None:intergenic 35.0%
GACTATCGATAGTTTCACAT+TGG + chr8_3:57698683-57698702 Msa1297400:five_prime_UTR 35.0%
TAGTTTGAATCTTGATCGGA+TGG + chr8_3:57699006-57699025 Msa1297400:intron 35.0%
AGAAAACCAGTTGGAGAATT+TGG + chr8_3:57699225-57699244 Msa1297400:CDS 35.0%
CGTGTTAAATGTATCCACAA+TGG - chr8_3:57698314-57698333 None:intergenic 35.0%
AAAAGCAGAAATAGCATGGT+GGG - chr8_3:57698406-57698425 None:intergenic 35.0%
AAAAAGCAGAAATAGCATGG+TGG - chr8_3:57698407-57698426 None:intergenic 35.0%
GACTATCGATAGTTTCACAT+TGG + chr8_3:57698683-57698702 Msa1297400:five_prime_UTR 35.0%
TAGTTTGAATCTTGATCGGA+TGG + chr8_3:57699006-57699025 Msa1297400:intron 35.0%
AGAAAACCAGTTGGAGAATT+TGG + chr8_3:57699225-57699244 Msa1297400:CDS 35.0%
TTGTGTGTGTGAAAGAGAGA+GGG - chr8_3:57698246-57698265 None:intergenic 40.0%
TTTGTGTGTGTGAAAGAGAG+AGG - chr8_3:57698247-57698266 None:intergenic 40.0%
!!! AAAGCAGGATTTTGATTGGC+TGG + chr8_3:57698576-57698595 Msa1297400:intron 40.0%
!! TGCTGTGCTATTTAAAGGGA+AGG - chr8_3:57698633-57698652 None:intergenic 40.0%
!! GCTGTGCTGTGCTATTTAAA+GGG - chr8_3:57698637-57698656 None:intergenic 40.0%
!! AGCTGTGCTGTGCTATTTAA+AGG - chr8_3:57698638-57698657 None:intergenic 40.0%
TCGCAATTATAAGGCTAAGC+TGG + chr8_3:57698770-57698789 Msa1297400:five_prime_UTR 40.0%
CAATTATAAGGCTAAGCTGG+CGG + chr8_3:57698773-57698792 Msa1297400:five_prime_UTR 40.0%
TGAGTGGTATTCAGAGATGT+GGG - chr8_3:57698879-57698898 None:intergenic 40.0%
CTTCAAATCCTCTCCTGTTT+CGG + chr8_3:57698907-57698926 Msa1297400:CDS 40.0%
ATGGCGAAGAAAAGAGAGAT+GGG - chr8_3:57698936-57698955 None:intergenic 40.0%
AATGGCGAAGAAAAGAGAGA+TGG - chr8_3:57698937-57698956 None:intergenic 40.0%
TTTCTTCGCCATTTGCATCT+TGG + chr8_3:57698944-57698963 Msa1297400:CDS 40.0%
AGTTACCTCCAAGATGCAAA+TGG - chr8_3:57698955-57698974 None:intergenic 40.0%
CATCTGGACCATTTCAAACT+AGG - chr8_3:57699074-57699093 None:intergenic 40.0%
CAAAAAGAATCGGTCACATC+TGG - chr8_3:57699090-57699109 None:intergenic 40.0%
AAAAGCGACGTTTACAGCTT+CGG + chr8_3:57699174-57699193 Msa1297400:CDS 40.0%
ATGGAGTAGACATTGTGCAA+TGG + chr8_3:57699250-57699269 Msa1297400:CDS 40.0%
TGGAGTAGACATTGTGCAAT+GGG + chr8_3:57699251-57699270 Msa1297400:CDS 40.0%
TCCTTCAGTTCCACTTGATT+TGG + chr8_3:57699329-57699348 Msa1297400:CDS 40.0%
CCTTCAGTTCCACTTGATTT+GGG + chr8_3:57699330-57699349 Msa1297400:CDS 40.0%
! CTTCAGTTCCACTTGATTTG+GGG + chr8_3:57699331-57699350 Msa1297400:CDS 40.0%
AACAAACACCCCAAATCAAG+TGG - chr8_3:57699342-57699361 None:intergenic 40.0%
TTGTGTGTGTGAAAGAGAGA+GGG - chr8_3:57698246-57698265 None:intergenic 40.0%
TTTGTGTGTGTGAAAGAGAG+AGG - chr8_3:57698247-57698266 None:intergenic 40.0%
!!! AAAGCAGGATTTTGATTGGC+TGG + chr8_3:57698576-57698595 Msa1297400:intron 40.0%
!! TGCTGTGCTATTTAAAGGGA+AGG - chr8_3:57698633-57698652 None:intergenic 40.0%
!! GCTGTGCTGTGCTATTTAAA+GGG - chr8_3:57698637-57698656 None:intergenic 40.0%
!! AGCTGTGCTGTGCTATTTAA+AGG - chr8_3:57698638-57698657 None:intergenic 40.0%
TCGCAATTATAAGGCTAAGC+TGG + chr8_3:57698770-57698789 Msa1297400:five_prime_UTR 40.0%
CAATTATAAGGCTAAGCTGG+CGG + chr8_3:57698773-57698792 Msa1297400:five_prime_UTR 40.0%
TGAGTGGTATTCAGAGATGT+GGG - chr8_3:57698879-57698898 None:intergenic 40.0%
CTTCAAATCCTCTCCTGTTT+CGG + chr8_3:57698907-57698926 Msa1297400:CDS 40.0%
ATGGCGAAGAAAAGAGAGAT+GGG - chr8_3:57698936-57698955 None:intergenic 40.0%
AATGGCGAAGAAAAGAGAGA+TGG - chr8_3:57698937-57698956 None:intergenic 40.0%
TTTCTTCGCCATTTGCATCT+TGG + chr8_3:57698944-57698963 Msa1297400:CDS 40.0%
AGTTACCTCCAAGATGCAAA+TGG - chr8_3:57698955-57698974 None:intergenic 40.0%
CATCTGGACCATTTCAAACT+AGG - chr8_3:57699074-57699093 None:intergenic 40.0%
CAAAAAGAATCGGTCACATC+TGG - chr8_3:57699090-57699109 None:intergenic 40.0%
AAAAGCGACGTTTACAGCTT+CGG + chr8_3:57699174-57699193 Msa1297400:CDS 40.0%
ATGGAGTAGACATTGTGCAA+TGG + chr8_3:57699250-57699269 Msa1297400:CDS 40.0%
TGGAGTAGACATTGTGCAAT+GGG + chr8_3:57699251-57699270 Msa1297400:CDS 40.0%
TCCTTCAGTTCCACTTGATT+TGG + chr8_3:57699329-57699348 Msa1297400:CDS 40.0%
CCTTCAGTTCCACTTGATTT+GGG + chr8_3:57699330-57699349 Msa1297400:CDS 40.0%
! CTTCAGTTCCACTTGATTTG+GGG + chr8_3:57699331-57699350 Msa1297400:CDS 40.0%
AACAAACACCCCAAATCAAG+TGG - chr8_3:57699342-57699361 None:intergenic 40.0%
TGTGAAAGAGAGAGGGAATG+GGG - chr8_3:57698239-57698258 None:intergenic 45.0%
GTGTGAAAGAGAGAGGGAAT+GGG - chr8_3:57698240-57698259 None:intergenic 45.0%
TGTGTGAAAGAGAGAGGGAA+TGG - chr8_3:57698241-57698260 None:intergenic 45.0%
GTCAGCAGCAACAAAAGGAA+GGG - chr8_3:57698365-57698384 None:intergenic 45.0%
! TGATTGGCTGGTGGTTCATT+CGG + chr8_3:57698588-57698607 Msa1297400:five_prime_UTR 45.0%
TGGTTCATTCGGGATAGTCT+CGG + chr8_3:57698599-57698618 Msa1297400:five_prime_UTR 45.0%
TAGTTTCGTGACGAAGACGA+TGG - chr8_3:57698748-57698767 None:intergenic 45.0%
AAGAGGATGAGTGGTAGAAG+GGG - chr8_3:57698818-57698837 None:intergenic 45.0%
GAAGAGGATGAGTGGTAGAA+GGG - chr8_3:57698819-57698838 None:intergenic 45.0%
CTTGGGTAAGGTTGAATGAG+AGG - chr8_3:57698844-57698863 None:intergenic 45.0%
!! GTGTGGAAAGTACTTGGGTA+AGG - chr8_3:57698856-57698875 None:intergenic 45.0%
GAGTGGTATTCAGAGATGTG+GGG - chr8_3:57698878-57698897 None:intergenic 45.0%
GTGAGTGGTATTCAGAGATG+TGG - chr8_3:57698880-57698899 None:intergenic 45.0%
ATTTGAAGGAGAGGAGTGAG+TGG - chr8_3:57698895-57698914 None:intergenic 45.0%
CGAAACAGGAGAGGATTTGA+AGG - chr8_3:57698909-57698928 None:intergenic 45.0%
!! GTACTTCTAGAGCTTGTAGC+AGG + chr8_3:57699201-57699220 Msa1297400:CDS 45.0%
GTAGCAGGAAGAAAACCAGT+TGG + chr8_3:57699216-57699235 Msa1297400:CDS 45.0%
CCATCACCAAATTCTCCAAC+TGG - chr8_3:57699234-57699253 None:intergenic 45.0%
! CCAGTTGGAGAATTTGGTGA+TGG + chr8_3:57699231-57699250 Msa1297400:CDS 45.0%
AGTGGAACTGAAGGAAGTCT+AGG - chr8_3:57699324-57699343 None:intergenic 45.0%
CCCAAATCAAGTGGAACTGA+AGG - chr8_3:57699333-57699352 None:intergenic 45.0%
TGTGAAAGAGAGAGGGAATG+GGG - chr8_3:57698239-57698258 None:intergenic 45.0%
GTGTGAAAGAGAGAGGGAAT+GGG - chr8_3:57698240-57698259 None:intergenic 45.0%
TGTGTGAAAGAGAGAGGGAA+TGG - chr8_3:57698241-57698260 None:intergenic 45.0%
GTCAGCAGCAACAAAAGGAA+GGG - chr8_3:57698365-57698384 None:intergenic 45.0%
! TGATTGGCTGGTGGTTCATT+CGG + chr8_3:57698588-57698607 Msa1297400:five_prime_UTR 45.0%
TGGTTCATTCGGGATAGTCT+CGG + chr8_3:57698599-57698618 Msa1297400:five_prime_UTR 45.0%
TAGTTTCGTGACGAAGACGA+TGG - chr8_3:57698748-57698767 None:intergenic 45.0%
AAGAGGATGAGTGGTAGAAG+GGG - chr8_3:57698818-57698837 None:intergenic 45.0%
GAAGAGGATGAGTGGTAGAA+GGG - chr8_3:57698819-57698838 None:intergenic 45.0%
CTTGGGTAAGGTTGAATGAG+AGG - chr8_3:57698844-57698863 None:intergenic 45.0%
!! GTGTGGAAAGTACTTGGGTA+AGG - chr8_3:57698856-57698875 None:intergenic 45.0%
GAGTGGTATTCAGAGATGTG+GGG - chr8_3:57698878-57698897 None:intergenic 45.0%
GTGAGTGGTATTCAGAGATG+TGG - chr8_3:57698880-57698899 None:intergenic 45.0%
ATTTGAAGGAGAGGAGTGAG+TGG - chr8_3:57698895-57698914 None:intergenic 45.0%
CGAAACAGGAGAGGATTTGA+AGG - chr8_3:57698909-57698928 None:intergenic 45.0%
!! GTACTTCTAGAGCTTGTAGC+AGG + chr8_3:57699201-57699220 Msa1297400:CDS 45.0%
GTAGCAGGAAGAAAACCAGT+TGG + chr8_3:57699216-57699235 Msa1297400:CDS 45.0%
CCATCACCAAATTCTCCAAC+TGG - chr8_3:57699234-57699253 None:intergenic 45.0%
! CCAGTTGGAGAATTTGGTGA+TGG + chr8_3:57699231-57699250 Msa1297400:CDS 45.0%
AGTGGAACTGAAGGAAGTCT+AGG - chr8_3:57699324-57699343 None:intergenic 45.0%
CCCAAATCAAGTGGAACTGA+AGG - chr8_3:57699333-57699352 None:intergenic 45.0%
!! TAGATTAAAAAAAATAAAAT+TGG - chr8_3:57699046-57699065 None:intergenic 5.0%
!! TAGATTAAAAAAAATAAAAT+TGG - chr8_3:57699046-57699065 None:intergenic 5.0%
CGTCAGCAGCAACAAAAGGA+AGG - chr8_3:57698366-57698385 None:intergenic 50.0%
GAGACGTCAGCAGCAACAAA+AGG - chr8_3:57698370-57698389 None:intergenic 50.0%
!!! GCAGGATTTTGATTGGCTGG+TGG + chr8_3:57698579-57698598 Msa1297400:intron 50.0%
! GATTGGCTGGTGGTTCATTC+GGG + chr8_3:57698589-57698608 Msa1297400:five_prime_UTR 50.0%
GGTTCATTCGGGATAGTCTC+GGG + chr8_3:57698600-57698619 Msa1297400:five_prime_UTR 50.0%
GGAAGAGGATGAGTGGTAGA+AGG - chr8_3:57698820-57698839 None:intergenic 50.0%
GGTTGAATGAGAGGAGGAAG+AGG - chr8_3:57698835-57698854 None:intergenic 50.0%
GGGTAAGGTTGAATGAGAGG+AGG - chr8_3:57698841-57698860 None:intergenic 50.0%
!! GTGGGGTGTGGAAAGTACTT+GGG - chr8_3:57698861-57698880 None:intergenic 50.0%
!! TGTGGGGTGTGGAAAGTACT+TGG - chr8_3:57698862-57698881 None:intergenic 50.0%
GTATTCAGAGATGTGGGGTG+TGG - chr8_3:57698873-57698892 None:intergenic 50.0%
CAGGAGAGGATTTGAAGGAG+AGG - chr8_3:57698904-57698923 None:intergenic 50.0%
CTTCGCCATTTGCATCTTGG+AGG + chr8_3:57698947-57698966 Msa1297400:CDS 50.0%
CGTCAGCAGCAACAAAAGGA+AGG - chr8_3:57698366-57698385 None:intergenic 50.0%
GAGACGTCAGCAGCAACAAA+AGG - chr8_3:57698370-57698389 None:intergenic 50.0%
!!! GCAGGATTTTGATTGGCTGG+TGG + chr8_3:57698579-57698598 Msa1297400:intron 50.0%
! GATTGGCTGGTGGTTCATTC+GGG + chr8_3:57698589-57698608 Msa1297400:five_prime_UTR 50.0%
GGTTCATTCGGGATAGTCTC+GGG + chr8_3:57698600-57698619 Msa1297400:five_prime_UTR 50.0%
GGAAGAGGATGAGTGGTAGA+AGG - chr8_3:57698820-57698839 None:intergenic 50.0%
GGTTGAATGAGAGGAGGAAG+AGG - chr8_3:57698835-57698854 None:intergenic 50.0%
GGGTAAGGTTGAATGAGAGG+AGG - chr8_3:57698841-57698860 None:intergenic 50.0%
!! GTGGGGTGTGGAAAGTACTT+GGG - chr8_3:57698861-57698880 None:intergenic 50.0%
!! TGTGGGGTGTGGAAAGTACT+TGG - chr8_3:57698862-57698881 None:intergenic 50.0%
GTATTCAGAGATGTGGGGTG+TGG - chr8_3:57698873-57698892 None:intergenic 50.0%
CAGGAGAGGATTTGAAGGAG+AGG - chr8_3:57698904-57698923 None:intergenic 50.0%
CTTCGCCATTTGCATCTTGG+AGG + chr8_3:57698947-57698966 Msa1297400:CDS 50.0%
GAGAGGAGGAAGAGGATGAG+TGG - chr8_3:57698827-57698846 None:intergenic 55.0%
ATGGGTGACCGAAACAGGAG+AGG - chr8_3:57698918-57698937 None:intergenic 55.0%
GAGAGATGGGTGACCGAAAC+AGG - chr8_3:57698923-57698942 None:intergenic 55.0%
CGACGTTTACAGCTTCGGTG+TGG + chr8_3:57699179-57699198 Msa1297400:CDS 55.0%
GAGAGGAGGAAGAGGATGAG+TGG - chr8_3:57698827-57698846 None:intergenic 55.0%
ATGGGTGACCGAAACAGGAG+AGG - chr8_3:57698918-57698937 None:intergenic 55.0%
GAGAGATGGGTGACCGAAAC+AGG - chr8_3:57698923-57698942 None:intergenic 55.0%
CGACGTTTACAGCTTCGGTG+TGG + chr8_3:57699179-57699198 Msa1297400:CDS 55.0%
AGGCTAAGCTGGCGGCAAAG+CGG + chr8_3:57698781-57698800 Msa1297400:five_prime_UTR 60.0%
AGGCTAAGCTGGCGGCAAAG+CGG + chr8_3:57698781-57698800 Msa1297400:five_prime_UTR 60.0%
CTGGCGGCAAAGCGGCGATG+AGG + chr8_3:57698789-57698808 Msa1297400:exon 70.0%
CTGGCGGCAAAGCGGCGATG+AGG + chr8_3:57698789-57698808 Msa1297400:exon 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 57698222 57699371 57698222 ID=Msa1297400;Name=Msa1297400
chr8_3 mRNA 57698222 57699371 57698222 ID=Msa1297400-mRNA-1;Parent=Msa1297400;Name=Msa1297400-mRNA-1;_AED=0.06;_eAED=0.06;_QI=349|1|0.66|1|0.5|0.33|3|0|128
chr8_3 exon 57698222 57698347 57698222 ID=Msa1297400-mRNA-1:exon:16535;Parent=Msa1297400-mRNA-1
chr8_3 exon 57698583 57698968 57698583 ID=Msa1297400-mRNA-1:exon:16536;Parent=Msa1297400-mRNA-1
chr8_3 exon 57699148 57699371 57699148 ID=Msa1297400-mRNA-1:exon:16537;Parent=Msa1297400-mRNA-1
chr8_3 five_prime_UTR 57698222 57698347 57698222 ID=Msa1297400-mRNA-1:five_prime_utr;Parent=Msa1297400-mRNA-1
chr8_3 five_prime_UTR 57698583 57698805 57698583 ID=Msa1297400-mRNA-1:five_prime_utr;Parent=Msa1297400-mRNA-1
chr8_3 CDS 57698806 57698968 57698806 ID=Msa1297400-mRNA-1:cds;Parent=Msa1297400-mRNA-1
chr8_3 CDS 57699148 57699371 57699148 ID=Msa1297400-mRNA-1:cds;Parent=Msa1297400-mRNA-1
Gene Sequence

>Msa1297400

ATGAGGCTTCCCCTTCTACCACTCATCCTCTTCCTCCTCTCATTCAACCTTACCCAAGTACTTTCCACACCCCACATCTCTGAATACCACTCACTCCTCTCCTTCAAATCCTCTCCTGTTTCGGTCACCCATCTCTCTTTTCTTCGCCATTTGCATCTTGGAGACGTTTACAGCTTGAAAGTTGATGAAAAAAGCGACGTTTACAGCTTCGGTGTGGTACTTCTAGAGCTTGTAGCAGGAAGAAAACCAGTTGGAGAATTTGGTGATGGAGTAGACATTGTGCAATGGGTGAGAAAAATGACAGACTCAAACAAAGGTGTTCTAAAAGTACTTGATCCTAGACTTCCTTCAGTTCCACTTGATTTGGGGTGTTTGTTTCAGCTTTAA
Protein sequence

>Msa1297400

MRLPLLPLILFLLSFNLTQVLSTPHISEYHSLLSFKSSPVSVTHLSFLRHLHLGDVYSLKVDEKSDVYSFGVVLLELVAGRKPVGEFGDGVDIVQWVRKMTDSNKGVLKVLDPRLPSVPLDLGCLFQL*