AlfalfaGEDB | Msa1300700

M. sativa cultivar zhongmu-4 / Msa1300700

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300700	I3S6K5	91.011	178	3	5	1	167	1	176	1.64e-106	311

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0060730	Msa1300700	-0.801083	1.064288e-48	-8.615850e-47
Msa0878220	Msa1300700	0.805567	1.253982e-49	-8.615850e-47
Msa0944020	Msa1300700	0.818774	1.644658e-52	-8.615850e-47
Msa1061740	Msa1300700	0.802067	6.689939e-49	-8.615850e-47
Msa1140630	Msa1300700	0.819866	9.275158e-53	-8.615850e-47
Msa1175100	Msa1300700	0.806563	7.742817e-50	-8.615850e-47
Msa1214750	Msa1300700	0.836049	1.174962e-56	-8.615850e-47
Msa1300700	Msa1336290	0.803972	2.701265e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300700	MtrunA17_Chr4g0063791	91.011	178	3	5	1	167	1	176	3.16e-110	311
Msa1300700	MtrunA17_Chr2g0333051	38.889	144	64	4	28	162	5	133	5.58e-19	78.2
Msa1300700	MtrunA17_Chr4g0003391	35.915	142	69	4	28	167	5	126	5.15e-14	65.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300700	AT3G15760.1	39.568	139	67	5	31	166	9	133	4.19e-16	71.2
Msa1300700	AT1G52565.1	39.552	134	59	4	28	159	8	121	1.02e-14	67.4

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Find 36 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGGTGAAGAGAGAAT+TGG	0.390105	8_3:-62354287	None:intergenic
AACAGAGCAGCTTTGTATAA+TGG	0.334899	8_3:+62354660	Msa1300700:CDS
AACATGGTGAAGAGAGAATT+GGG	0.400815	8_3:-62354286	None:intergenic
AAGGTTCAAAAGAATTGTAA+TGG	0.522701	8_3:+62354609	Msa1300700:CDS
AAGTACAGAAAACAACAACA+TGG	0.727013	8_3:+62354531	Msa1300700:CDS
AATGGTGGAAACGAGAACAG+AGG	0.717333	8_3:+62354627	Msa1300700:CDS
AATTCACCTTAACAGAATAG+AGG	0.541511	8_3:-62354898	None:intergenic
AGAATTGGGATTTCTGAAGA+CGG	0.611480	8_3:-62354272	None:intergenic
AGATCGTGTGAATCAACGAT+TGG	0.545757	8_3:+62354692	Msa1300700:CDS
AGCAAGGATGATGACTGTGC+CGG	0.571426	8_3:-62354247	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGATGAAAAGTGAGAAAG+TGG	-	chr8_3:62354143-62354162	None:intergenic	30.0%
TGAAGAAAAGAAGAAAGGAT+TGG	-	chr8_3:62354168-62354187	None:intergenic	30.0%
AAGAAGAAGAAGAAGAAACA+TGG	-	chr8_3:62354305-62354324	None:intergenic	30.0%
GATAAACAAGAACGTAGAAT+AGG	-	chr8_3:62354336-62354355	None:intergenic	30.0%
AGTAAAAAGTAACTTACCAG+AGG	-	chr8_3:62354358-62354377	None:intergenic	30.0%
AAGTACAGAAAACAACAACA+TGG	+	chr8_3:62354531-62354550	Msa1300700:CDS	30.0%
GTTCAAAAGAATTGTAATGG+TGG	+	chr8_3:62354612-62354631	Msa1300700:CDS	30.0%
AATTCACCTTAACAGAATAG+AGG	-	chr8_3:62354901-62354920	None:intergenic	30.0%
CTATTCTGTTAAGGTGAATT+AGG	+	chr8_3:62354901-62354920	Msa1300700:three_prime_UTR	30.0%
CCGAACTAAAATTTGAACAT+TGG	-	chr8_3:62355052-62355071	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	62354110	62355120	62354110	ID=Msa1300700;Name=Msa1300700
chr8_3	mRNA	62354110	62355120	62354110	ID=Msa1300700-mRNA-1;Parent=Msa1300700;Name=Msa1300700-mRNA-1;_AED=0.05;_eAED=0.05;_QI=19\|1\|1\|1\|1\|1\|2\|395\|167
chr8_3	exon	62354110	62354360	62354110	ID=Msa1300700-mRNA-1:exon:18507;Parent=Msa1300700-mRNA-1
chr8_3	exon	62354454	62355120	62354454	ID=Msa1300700-mRNA-1:exon:18508;Parent=Msa1300700-mRNA-1
chr8_3	five_prime_UTR	62354110	62354128	62354110	ID=Msa1300700-mRNA-1:five_prime_utr;Parent=Msa1300700-mRNA-1
chr8_3	CDS	62354129	62354360	62354129	ID=Msa1300700-mRNA-1:cds;Parent=Msa1300700-mRNA-1
chr8_3	CDS	62354454	62354725	62354454	ID=Msa1300700-mRNA-1:cds;Parent=Msa1300700-mRNA-1
chr8_3	three_prime_UTR	62354726	62355120	62354726	ID=Msa1300700-mRNA-1:three_prime_utr;Parent=Msa1300700-mRNA-1

Gene Sequence

>Msa1300700

ATGAGACTATACCACTTTCTCACTTTTCATCAAAGACCAATCCTTTCTTCTTTTCTTCAAAACATGTCTTCAATGATTACCTCACAAGGTGCTGTCTTTGCAACCGCCATGGCTGTCTCCGGCACAGTCATCATCCTTGCTCTCCGTCTTCAGAAATCCCAATTCTCTCTTCACCATGTTTCTTCTTCTTCTTCTTCTTCAACTCCTATTCTACGTTCTTGTTTATCCTCTGATGAAAAGAAAACAAAGAAACTGAAGAAAAGAGTTAGTTTTGCTAAGGATGTTGTGGATTCATGTAAAGATGGAGAAAAGTACAGAAAACAACAACATGGTACTAGTACTATGAATAATACTACTAAACATTCTTTGAAATTGAATTCAAGATCAAAGGTTCAAAAGAATTGTAATGGTGGAAACGAGAACAGAGGAATGCCTGCAAACAGAGCAGCTTTGTATAATGGAATTCTTAGAGATCGTGTGAATCAACGATTGGCTTATTGTTGA

Protein sequence

>Msa1300700

MRLYHFLTFHQRPILSSFLQNMSSMITSQGAVFATAMAVSGTVIILALRLQKSQFSLHHVSSSSSSSTPILRSCLSSDEKKTKKLKKRVSFAKDVVDSCKDGEKYRKQQHGTSTMNNTTKHSLKLNSRSKVQKNCNGGNENRGMPANRAALYNGILRDRVNQRLAYC*