AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1175100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1175100 MtrunA17_Chr8g0345171 96.269 134 4 1 1 134 1 133 6.33e-94 266
Msa1175100 MtrunA17_Chr7g0276621 55.469 128 43 6 1 123 23 141 1.01e-37 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1175100 AT3G14280.1 39.815 108 57 3 21 128 21 120 2.84e-15 68.2

Find 29 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTTGAATTGCAATAAAA+TGG 0.221285 8_1:-6376465 Msa1175100:exon
TTCTTGAAGAATTAGCTTAA+TGG 0.238281 8_1:-6377500 Msa1175100:exon
GAATTATCATATTTCTGAAT+TGG 0.275390 8_1:+6377448 None:intergenic
AAGAAGAAGATGAGATAATA+AGG 0.331139 8_1:+6377537 None:intergenic
AATTATCATATTTCTGAATT+GGG 0.345181 8_1:+6377449 None:intergenic
TTGTCTCACTCATAGCTTTG+AGG 0.351441 8_1:-6376412 Msa1175100:three_prime_UTR
AGTTGGTTTCAAATTGCAAA+AGG 0.405294 8_1:-6376535 Msa1175100:CDS
CTTTGGAGCATAGCATCGTT+TGG 0.411384 8_1:+6377393 None:intergenic
TGAATCTTCTAAAACAAAGT+TGG 0.441522 8_1:-6376552 Msa1175100:CDS
CTTGAAATTTGTCTATAAGA+TGG 0.456782 8_1:+6376873 None:intergenic
TCAAATAGCACATGTGTAGT+AGG 0.464126 8_1:-6376343 Msa1175100:three_prime_UTR
TTATTTCTCCACTCTCATCT+TGG 0.480024 8_1:+6376919 None:intergenic
GTTGGTTTCAAATTGCAAAA+GGG 0.500062 8_1:-6376534 Msa1175100:CDS
ATTTGTGAAGGGTATGATGA+AGG 0.501766 8_1:-6376897 Msa1175100:CDS
GACCAACGGTTCATCACAAA+AGG 0.504204 8_1:-6376851 Msa1175100:intron
GAAATTGATGATGCAATTGA+AGG 0.528412 8_1:-6376508 Msa1175100:CDS
GTGGAGAAATAATTTGTGAA+GGG 0.550320 8_1:-6376908 Msa1175100:CDS
AGTGGAGAAATAATTTGTGA+AGG 0.561503 8_1:-6376909 Msa1175100:CDS
TTTGTGAAGGGTATGATGAA+GGG 0.566441 8_1:-6376896 Msa1175100:CDS
CACAGCATCCATCAAGAACA+AGG 0.570428 8_1:-6377335 Msa1175100:intron
ATTCTTCAAGAACAAGAAAG+AGG 0.573168 8_1:+6377511 None:intergenic
AAGAAGATGAGATAATAAGG+AGG 0.575986 8_1:+6377540 None:intergenic
TAGACAAATTTCAAGACCAA+CGG 0.578411 8_1:-6376865 Msa1175100:CDS
TGAAGGTGGTCGTTTGCAAG+AGG 0.580771 8_1:-6376491 Msa1175100:CDS
AAAGTCAACAGTTGGTCCAG+TGG 0.602548 8_1:-6377368 Msa1175100:CDS
CAAAGCTATGAGTGAGACAA+AGG 0.622287 8_1:+6376415 None:intergenic
ATTTGCTACCAAGATGAGAG+TGG 0.633835 8_1:-6376927 Msa1175100:CDS
CTTCAAGAACAAGAAAGAGG+TGG 0.639571 8_1:+6377514 None:intergenic
ATTGATGATGCAATTGAAGG+TGG 0.706142 8_1:-6376505 Msa1175100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATCATATTTCTGAATT+GGG + chr8_1:6376414-6376433 None:intergenic 15.0%
!! AAGAATAGATAAAAGATAAT+AGG + chr8_1:6376558-6376577 None:intergenic 15.0%
!! ATAGATATATGTATATCAAT+TGG + chr8_1:6376601-6376620 None:intergenic 15.0%
!! AAAAAAGATGAAAAAAACTT+TGG - chr8_1:6376647-6376666 Msa1175100:intron 15.0%
!!! AGATAAGTTTTTTCTTTTTT+TGG - chr8_1:6376767-6376786 Msa1175100:intron 15.0%
!!! AGAATAAAATTGTCATTTTA+TGG + chr8_1:6377093-6377112 None:intergenic 15.0%
!!! AAAATGACAATTTTATTCTT+CGG - chr8_1:6377094-6377113 Msa1175100:intron 15.0%
!! TAATTTGAATTGCAATAAAA+TGG - chr8_1:6377395-6377414 Msa1175100:CDS 15.0%
!!! AATTATCATATTTCTGAATT+GGG + chr8_1:6376414-6376433 None:intergenic 15.0%
!! AAGAATAGATAAAAGATAAT+AGG + chr8_1:6376558-6376577 None:intergenic 15.0%
!! ATAGATATATGTATATCAAT+TGG + chr8_1:6376601-6376620 None:intergenic 15.0%
!! AAAAAAGATGAAAAAAACTT+TGG - chr8_1:6376647-6376666 Msa1175100:intron 15.0%
!!! AGATAAGTTTTTTCTTTTTT+TGG - chr8_1:6376767-6376786 Msa1175100:intron 15.0%
!!! AGAATAAAATTGTCATTTTA+TGG + chr8_1:6377093-6377112 None:intergenic 15.0%
!!! AAAATGACAATTTTATTCTT+CGG - chr8_1:6377094-6377113 Msa1175100:intron 15.0%
!! TAATTTGAATTGCAATAAAA+TGG - chr8_1:6377395-6377414 Msa1175100:CDS 15.0%
!!! GAATTATCATATTTCTGAAT+TGG + chr8_1:6376415-6376434 None:intergenic 20.0%
!!! AAAAAAACTTTGGTTAGTTA+TGG - chr8_1:6376657-6376676 Msa1175100:intron 20.0%
!!! TACCTTATAGTTTTTTCTAA+GGG + chr8_1:6376735-6376754 None:intergenic 20.0%
!!! TGGATATATCTACTTTTTAT+TGG - chr8_1:6376787-6376806 Msa1175100:intron 20.0%
!! AATTGATGTTTATATTCTGA+AGG - chr8_1:6377057-6377076 Msa1175100:intron 20.0%
!! ATATAAATATGTCTTACCAA+TGG - chr8_1:6377252-6377271 Msa1175100:intron 20.0%
!!! GAATTATCATATTTCTGAAT+TGG + chr8_1:6376415-6376434 None:intergenic 20.0%
!!! AAAAAAACTTTGGTTAGTTA+TGG - chr8_1:6376657-6376676 Msa1175100:intron 20.0%
!!! TACCTTATAGTTTTTTCTAA+GGG + chr8_1:6376735-6376754 None:intergenic 20.0%
!!! TGGATATATCTACTTTTTAT+TGG - chr8_1:6376787-6376806 Msa1175100:intron 20.0%
!! AATTGATGTTTATATTCTGA+AGG - chr8_1:6377057-6377076 Msa1175100:intron 20.0%
!! ATATAAATATGTCTTACCAA+TGG - chr8_1:6377252-6377271 Msa1175100:intron 20.0%
! AATAAGGAGGAAAAATGAAA+TGG + chr8_1:6376310-6376329 None:intergenic 25.0%
! AAGAAGAAGATGAGATAATA+AGG + chr8_1:6376326-6376345 None:intergenic 25.0%
!! TTCTTGAAGAATTAGCTTAA+TGG - chr8_1:6376360-6376379 Msa1175100:three_prime_UTR 25.0%
!! TTTGGTTAGTTATGGAAATT+TGG - chr8_1:6376665-6376684 Msa1175100:intron 25.0%
! TTTGTCTCTAGTTATATCTT+TGG - chr8_1:6376704-6376723 Msa1175100:intron 25.0%
!!! GTACCTTATAGTTTTTTCTA+AGG + chr8_1:6376736-6376755 None:intergenic 25.0%
! TTTCTCCTAGTAGTAAAAAT+AGG - chr8_1:6376823-6376842 Msa1175100:intron 25.0%
! TGTCTAATTTGATACTAAAC+AGG - chr8_1:6376881-6376900 Msa1175100:CDS 25.0%
!!! TACTAAACAGGTTTTTTATG+AGG - chr8_1:6376893-6376912 Msa1175100:CDS 25.0%
! CTTGAAATTTGTCTATAAGA+TGG + chr8_1:6376990-6377009 None:intergenic 25.0%
!!! TGAATCTTCTAAAACAAAGT+TGG - chr8_1:6377308-6377327 Msa1175100:intron 25.0%
! AATAAGGAGGAAAAATGAAA+TGG + chr8_1:6376310-6376329 None:intergenic 25.0%
! AAGAAGAAGATGAGATAATA+AGG + chr8_1:6376326-6376345 None:intergenic 25.0%
!! TTCTTGAAGAATTAGCTTAA+TGG - chr8_1:6376360-6376379 Msa1175100:three_prime_UTR 25.0%
!! TTTGGTTAGTTATGGAAATT+TGG - chr8_1:6376665-6376684 Msa1175100:intron 25.0%
! TTTGTCTCTAGTTATATCTT+TGG - chr8_1:6376704-6376723 Msa1175100:intron 25.0%
!!! GTACCTTATAGTTTTTTCTA+AGG + chr8_1:6376736-6376755 None:intergenic 25.0%
! TTTCTCCTAGTAGTAAAAAT+AGG - chr8_1:6376823-6376842 Msa1175100:intron 25.0%
! TGTCTAATTTGATACTAAAC+AGG - chr8_1:6376881-6376900 Msa1175100:CDS 25.0%
!!! TACTAAACAGGTTTTTTATG+AGG - chr8_1:6376893-6376912 Msa1175100:CDS 25.0%
! CTTGAAATTTGTCTATAAGA+TGG + chr8_1:6376990-6377009 None:intergenic 25.0%
!!! TGAATCTTCTAAAACAAAGT+TGG - chr8_1:6377308-6377327 Msa1175100:intron 25.0%
AAGAAGATGAGATAATAAGG+AGG + chr8_1:6376323-6376342 None:intergenic 30.0%
ATTCTTCAAGAACAAGAAAG+AGG + chr8_1:6376352-6376371 None:intergenic 30.0%
!! GATTTTAACCTTGTTCTTGA+TGG + chr8_1:6376536-6376555 None:intergenic 30.0%
GTCCCTTAGAAAAAACTATA+AGG - chr8_1:6376730-6376749 Msa1175100:intron 30.0%
ACTATAAGGTACGTCAAAAT+TGG - chr8_1:6376744-6376763 Msa1175100:intron 30.0%
! AGTGGAGAAATAATTTGTGA+AGG - chr8_1:6376951-6376970 Msa1175100:CDS 30.0%
! GTGGAGAAATAATTTGTGAA+GGG - chr8_1:6376952-6376971 Msa1175100:CDS 30.0%
TAGACAAATTTCAAGACCAA+CGG - chr8_1:6376995-6377014 Msa1175100:intron 30.0%
! ATGACAATTTTATTCTTCGG+CGG - chr8_1:6377097-6377116 Msa1175100:intron 30.0%
TGCTACCAGTTAAAATCTTT+CGG - chr8_1:6377125-6377144 Msa1175100:intron 30.0%
AAATGTCGATGAGGTTAATT+TGG - chr8_1:6377153-6377172 Msa1175100:intron 30.0%
!! AGGTTAATTTGGTATTTTCG+TGG - chr8_1:6377164-6377183 Msa1175100:intron 30.0%
! TTTCTAGCATTCTTTGATGT+CGG - chr8_1:6377190-6377209 Msa1175100:intron 30.0%
AGCACAACAAAAAATTCCAT+TGG + chr8_1:6377271-6377290 None:intergenic 30.0%
!!! TGGAATTTTTTGTTGTGCTT+AGG - chr8_1:6377272-6377291 Msa1175100:intron 30.0%
!!! GGAATTTTTTGTTGTGCTTA+GGG - chr8_1:6377273-6377292 Msa1175100:intron 30.0%
! AGTTGGTTTCAAATTGCAAA+AGG - chr8_1:6377325-6377344 Msa1175100:intron 30.0%
! GTTGGTTTCAAATTGCAAAA+GGG - chr8_1:6377326-6377345 Msa1175100:intron 30.0%
!! GAAATTGATGATGCAATTGA+AGG - chr8_1:6377352-6377371 Msa1175100:CDS 30.0%
TAAAATGAAAAATGTGCTGC+AGG + chr8_1:6377549-6377568 None:intergenic 30.0%
AAGAAGATGAGATAATAAGG+AGG + chr8_1:6376323-6376342 None:intergenic 30.0%
ATTCTTCAAGAACAAGAAAG+AGG + chr8_1:6376352-6376371 None:intergenic 30.0%
!! GATTTTAACCTTGTTCTTGA+TGG + chr8_1:6376536-6376555 None:intergenic 30.0%
GTCCCTTAGAAAAAACTATA+AGG - chr8_1:6376730-6376749 Msa1175100:intron 30.0%
ACTATAAGGTACGTCAAAAT+TGG - chr8_1:6376744-6376763 Msa1175100:intron 30.0%
! AGTGGAGAAATAATTTGTGA+AGG - chr8_1:6376951-6376970 Msa1175100:CDS 30.0%
! GTGGAGAAATAATTTGTGAA+GGG - chr8_1:6376952-6376971 Msa1175100:CDS 30.0%
TAGACAAATTTCAAGACCAA+CGG - chr8_1:6376995-6377014 Msa1175100:intron 30.0%
! ATGACAATTTTATTCTTCGG+CGG - chr8_1:6377097-6377116 Msa1175100:intron 30.0%
TGCTACCAGTTAAAATCTTT+CGG - chr8_1:6377125-6377144 Msa1175100:intron 30.0%
AAATGTCGATGAGGTTAATT+TGG - chr8_1:6377153-6377172 Msa1175100:intron 30.0%
!! AGGTTAATTTGGTATTTTCG+TGG - chr8_1:6377164-6377183 Msa1175100:intron 30.0%
! TTTCTAGCATTCTTTGATGT+CGG - chr8_1:6377190-6377209 Msa1175100:intron 30.0%
AGCACAACAAAAAATTCCAT+TGG + chr8_1:6377271-6377290 None:intergenic 30.0%
!!! TGGAATTTTTTGTTGTGCTT+AGG - chr8_1:6377272-6377291 Msa1175100:intron 30.0%
!!! GGAATTTTTTGTTGTGCTTA+GGG - chr8_1:6377273-6377292 Msa1175100:intron 30.0%
! AGTTGGTTTCAAATTGCAAA+AGG - chr8_1:6377325-6377344 Msa1175100:intron 30.0%
! GTTGGTTTCAAATTGCAAAA+GGG - chr8_1:6377326-6377345 Msa1175100:intron 30.0%
!! GAAATTGATGATGCAATTGA+AGG - chr8_1:6377352-6377371 Msa1175100:CDS 30.0%
TAAAATGAAAAATGTGCTGC+AGG + chr8_1:6377549-6377568 None:intergenic 30.0%
!!! CCAACTGTTGACTTTTTCTT+TGG + chr8_1:6376487-6376506 None:intergenic 35.0%
CCAAAGAAAAAGTCAACAGT+TGG - chr8_1:6376484-6376503 Msa1175100:CDS 35.0%
!!! CCTAGCCTATTTTTACTACT+AGG + chr8_1:6376831-6376850 None:intergenic 35.0%
CCTAGTAGTAAAAATAGGCT+AGG - chr8_1:6376828-6376847 Msa1175100:intron 35.0%
! TTTTATGAGGATGAGTCTCA+AGG - chr8_1:6376906-6376925 Msa1175100:CDS 35.0%
! TTATTTCTCCACTCTCATCT+TGG + chr8_1:6376944-6376963 None:intergenic 35.0%
! ATTTGTGAAGGGTATGATGA+AGG - chr8_1:6376963-6376982 Msa1175100:intron 35.0%
! TTTGTGAAGGGTATGATGAA+GGG - chr8_1:6376964-6376983 Msa1175100:intron 35.0%
TACCAGTTAAAATCTTTCGG+CGG - chr8_1:6377128-6377147 Msa1175100:intron 35.0%
!! ATTGATGATGCAATTGAAGG+TGG - chr8_1:6377355-6377374 Msa1175100:CDS 35.0%
TCAAATAGCACATGTGTAGT+AGG - chr8_1:6377517-6377536 Msa1175100:five_prime_UTR 35.0%
!!! CCAACTGTTGACTTTTTCTT+TGG + chr8_1:6376487-6376506 None:intergenic 35.0%
CCAAAGAAAAAGTCAACAGT+TGG - chr8_1:6376484-6376503 Msa1175100:CDS 35.0%
!!! CCTAGCCTATTTTTACTACT+AGG + chr8_1:6376831-6376850 None:intergenic 35.0%
CCTAGTAGTAAAAATAGGCT+AGG - chr8_1:6376828-6376847 Msa1175100:intron 35.0%
! TTTTATGAGGATGAGTCTCA+AGG - chr8_1:6376906-6376925 Msa1175100:CDS 35.0%
! TTATTTCTCCACTCTCATCT+TGG + chr8_1:6376944-6376963 None:intergenic 35.0%
! ATTTGTGAAGGGTATGATGA+AGG - chr8_1:6376963-6376982 Msa1175100:intron 35.0%
! TTTGTGAAGGGTATGATGAA+GGG - chr8_1:6376964-6376983 Msa1175100:intron 35.0%
TACCAGTTAAAATCTTTCGG+CGG - chr8_1:6377128-6377147 Msa1175100:intron 35.0%
!! ATTGATGATGCAATTGAAGG+TGG - chr8_1:6377355-6377374 Msa1175100:CDS 35.0%
TCAAATAGCACATGTGTAGT+AGG - chr8_1:6377517-6377536 Msa1175100:five_prime_UTR 35.0%
CTTCAAGAACAAGAAAGAGG+TGG + chr8_1:6376349-6376368 None:intergenic 40.0%
! GCTGTGATTTTCAATACCAC+TGG + chr8_1:6376511-6376530 None:intergenic 40.0%
! ATTTGCTACCAAGATGAGAG+TGG - chr8_1:6376933-6376952 Msa1175100:CDS 40.0%
! TACCTTTTGTGATGAACCGT+TGG + chr8_1:6377014-6377033 None:intergenic 40.0%
!! AACCGCCGAAAGATTTTAAC+TGG + chr8_1:6377133-6377152 None:intergenic 40.0%
CAAAGCTATGAGTGAGACAA+AGG + chr8_1:6377448-6377467 None:intergenic 40.0%
! TTGTCTCACTCATAGCTTTG+AGG - chr8_1:6377448-6377467 Msa1175100:CDS 40.0%
CTTCAAGAACAAGAAAGAGG+TGG + chr8_1:6376349-6376368 None:intergenic 40.0%
! GCTGTGATTTTCAATACCAC+TGG + chr8_1:6376511-6376530 None:intergenic 40.0%
! ATTTGCTACCAAGATGAGAG+TGG - chr8_1:6376933-6376952 Msa1175100:CDS 40.0%
! TACCTTTTGTGATGAACCGT+TGG + chr8_1:6377014-6377033 None:intergenic 40.0%
!! AACCGCCGAAAGATTTTAAC+TGG + chr8_1:6377133-6377152 None:intergenic 40.0%
CAAAGCTATGAGTGAGACAA+AGG + chr8_1:6377448-6377467 None:intergenic 40.0%
! TTGTCTCACTCATAGCTTTG+AGG - chr8_1:6377448-6377467 Msa1175100:CDS 40.0%
!! CTTTGGAGCATAGCATCGTT+TGG + chr8_1:6376470-6376489 None:intergenic 45.0%
AAAGTCAACAGTTGGTCCAG+TGG - chr8_1:6376492-6376511 Msa1175100:CDS 45.0%
CACAGCATCCATCAAGAACA+AGG - chr8_1:6376525-6376544 Msa1175100:CDS 45.0%
GACCAACGGTTCATCACAAA+AGG - chr8_1:6377009-6377028 Msa1175100:intron 45.0%
!! CTTTGGAGCATAGCATCGTT+TGG + chr8_1:6376470-6376489 None:intergenic 45.0%
AAAGTCAACAGTTGGTCCAG+TGG - chr8_1:6376492-6376511 Msa1175100:CDS 45.0%
CACAGCATCCATCAAGAACA+AGG - chr8_1:6376525-6376544 Msa1175100:CDS 45.0%
GACCAACGGTTCATCACAAA+AGG - chr8_1:6377009-6377028 Msa1175100:intron 45.0%
TCGGCGGTTAAATGTCGATG+AGG - chr8_1:6377144-6377163 Msa1175100:intron 50.0%
TGAAGGTGGTCGTTTGCAAG+AGG - chr8_1:6377369-6377388 Msa1175100:CDS 50.0%
TCGGCGGTTAAATGTCGATG+AGG - chr8_1:6377144-6377163 Msa1175100:intron 50.0%
TGAAGGTGGTCGTTTGCAAG+AGG - chr8_1:6377369-6377388 Msa1175100:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 6376306 6377576 6376306 ID=Msa1175100;Name=Msa1175100
chr8_1 mRNA 6376306 6377576 6376306 ID=Msa1175100-mRNA-1;Parent=Msa1175100;Name=Msa1175100-mRNA-1;_AED=0.06;_eAED=0.06;_QI=73|1|1|1|1|1|3|174|134
chr8_1 exon 6376306 6376588 6376306 ID=Msa1175100-mRNA-1:exon:2207;Parent=Msa1175100-mRNA-1
chr8_1 exon 6376852 6376979 6376852 ID=Msa1175100-mRNA-1:exon:2206;Parent=Msa1175100-mRNA-1
chr8_1 exon 6377336 6377576 6377336 ID=Msa1175100-mRNA-1:exon:2205;Parent=Msa1175100-mRNA-1
chr8_1 five_prime_UTR 6377504 6377576 6377504 ID=Msa1175100-mRNA-1:five_prime_utr;Parent=Msa1175100-mRNA-1
chr8_1 CDS 6377336 6377503 6377336 ID=Msa1175100-mRNA-1:cds;Parent=Msa1175100-mRNA-1
chr8_1 CDS 6376852 6376979 6376852 ID=Msa1175100-mRNA-1:cds;Parent=Msa1175100-mRNA-1
chr8_1 CDS 6376480 6376588 6376480 ID=Msa1175100-mRNA-1:cds;Parent=Msa1175100-mRNA-1
chr8_1 three_prime_UTR 6376306 6376479 6376306 ID=Msa1175100-mRNA-1:three_prime_utr;Parent=Msa1175100-mRNA-1
Gene Sequence

>Msa1175100

ATGGCTGTTCACTGCAATTCTTTAGCTCAAATCCCAATTCAGAAATATGATAATTCATTTGTGAAATATTCAAACAATTTGTTGATGTCCAAACGATGCTATGCTCCAAAGAAAAAGTCAACAGTTGGTCCAGTGGTATTGAAAATCACAGCATCCATCAAGAACAAGGTTTTTTATGAGGATGAGTCTCAAGGTATAATTTGCTACCAAGATGAGAGTGGAGAAATAATTTGTGAAGGGTATGATGAAGGGCCATCTTATAGACAAATTTCAAGACCAACGGTTCATCACAAAAGGGATGTAGAAATTGTGAATCTTCTAAAACAAAGTTGGTTTCAAATTGCAAAAGGGAAAGAAATTGATGATGCAATTGAAGGTGGTCGTTTGCAAGAGGATTTAATTTGA

Protein sequence

>Msa1175100

MAVHCNSLAQIPIQKYDNSFVKYSNNLLMSKRCYAPKKKSTVGPVVLKITASIKNKVFYEDESQGIICYQDESGEIICEGYDEGPSYRQISRPTVHHKRDVEIVNLLKQSWFQIAKGKEIDDAIEGGRLQEDLI*