AlfalfaGEDB | Msa0818310

M. sativa cultivar zhongmu-4 / Msa0818310

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa0818310	0.840033	1.109567e-57	-8.615850e-47
Msa0008410	Msa0818310	0.802832	4.652543e-49	-8.615850e-47
Msa0014990	Msa0818310	0.829531	4.886312e-55	-8.615850e-47
Msa0017770	Msa0818310	-0.812380	4.365111e-51	-8.615850e-47
Msa0026130	Msa0818310	0.844965	5.452345e-59	-8.615850e-47
Msa0027260	Msa0818310	0.847984	8.183563e-60	-8.615850e-47
Msa0028420	Msa0818310	0.811639	6.329762e-51	-8.615850e-47
Msa0029630	Msa0818310	0.801005	1.104405e-48	-8.615850e-47
Msa0032260	Msa0818310	0.821425	4.066086e-53	-8.615850e-47
Msa0034420	Msa0818310	0.804826	1.792395e-49	-8.615850e-47
Msa0046740	Msa0818310	0.816885	4.390580e-52	-8.615850e-47
Msa0048180	Msa0818310	0.803782	2.958267e-49	-8.615850e-47
Msa0056430	Msa0818310	0.802814	4.691715e-49	-8.615850e-47
Msa0056470	Msa0818310	0.852021	6.064832e-61	-8.615850e-47
Msa0064350	Msa0818310	0.828472	8.821560e-55	-8.615850e-47
Msa0064380	Msa0818310	0.817587	3.052982e-52	-8.615850e-47
Msa0069330	Msa0818310	0.825162	5.448459e-54	-8.615850e-47
Msa0072370	Msa0818310	0.804256	2.357044e-49	-8.615850e-47
Msa0076040	Msa0818310	0.846990	1.534926e-59	-8.615850e-47
Msa0078650	Msa0818310	0.811385	7.188975e-51	-8.615850e-47
Msa0082280	Msa0818310	0.838681	2.488608e-57	-8.615850e-47
Msa0083070	Msa0818310	0.814226	1.715757e-51	-8.615850e-47
Msa0094470	Msa0818310	0.808558	2.919357e-50	-8.615850e-47
Msa0104550	Msa0818310	0.815736	7.932938e-52	-8.615850e-47
Msa0112790	Msa0818310	0.828243	1.001858e-54	-8.615850e-47
Msa0115990	Msa0818310	0.801386	9.226832e-49	-8.615850e-47
Msa0608320	Msa0818310	0.800450	1.432860e-48	-8.615850e-47
Msa0621950	Msa0818310	0.807590	4.692237e-50	-8.615850e-47
Msa0651050	Msa0818310	0.812690	3.734750e-51	-8.615850e-47
Msa0661300	Msa0818310	0.831430	1.676711e-55	-8.615850e-47
Msa0662500	Msa0818310	0.828267	9.884373e-55	-8.615850e-47
Msa0685460	Msa0818310	0.823763	1.162624e-53	-8.615850e-47
Msa0687630	Msa0818310	0.821924	3.116865e-53	-8.615850e-47
Msa0689230	Msa0818310	0.852581	4.203018e-61	-8.615850e-47
Msa0690150	Msa0818310	0.831479	1.630964e-55	-8.615850e-47
Msa0692840	Msa0818310	0.874679	5.593320e-68	-8.615850e-47
Msa0692940	Msa0818310	0.828262	9.914948e-55	-8.615850e-47
Msa0703230	Msa0818310	0.805257	1.456409e-49	-8.615850e-47
Msa0709500	Msa0818310	0.806978	6.325451e-50	-8.615850e-47
Msa0716690	Msa0818310	0.814656	1.378764e-51	-8.615850e-47
Msa0719510	Msa0818310	0.820847	5.523834e-53	-8.615850e-47
Msa0720820	Msa0818310	0.804595	2.003205e-49	-8.615850e-47
Msa0746420	Msa0818310	0.814546	1.457923e-51	-8.615850e-47
Msa0749760	Msa0818310	0.848965	4.379126e-60	-8.615850e-47
Msa0750880	Msa0818310	0.849607	2.901061e-60	-8.615850e-47
Msa0755340	Msa0818310	0.810243	1.269764e-50	-8.615850e-47
Msa0756260	Msa0818310	0.830443	2.928313e-55	-8.615850e-47
Msa0761940	Msa0818310	0.821033	5.004975e-53	-8.615850e-47
Msa0763020	Msa0818310	0.814934	1.196075e-51	-8.615850e-47
Msa0763070	Msa0818310	0.825489	4.559806e-54	-8.615850e-47
Msa0784200	Msa0818310	0.987738	2.364369e-171	-8.615850e-47
Msa0790850	Msa0818310	0.802435	5.617299e-49	-8.615850e-47
Msa0818310	Msa0830090	0.814089	1.839849e-51	-8.615850e-47
Msa0818310	Msa0841940	0.816417	5.589693e-52	-8.615850e-47
Msa0818310	Msa0858770	0.987830	1.074991e-171	-8.615850e-47
Msa0818310	Msa0881290	0.839696	1.357531e-57	-8.615850e-47
Msa0818310	Msa0896540	0.801330	9.474497e-49	-8.615850e-47
Msa0818310	Msa0902760	0.871867	4.926580e-67	-8.615850e-47
Msa0818310	Msa0925440	0.806516	7.920329e-50	-8.615850e-47
Msa0818310	Msa0931460	0.819889	9.163070e-53	-8.615850e-47
Msa0818310	Msa0936340	0.812752	3.619472e-51	-8.615850e-47
Msa0818310	Msa0953310	0.819036	1.433784e-52	-8.615850e-47
Msa0818310	Msa0968590	0.857243	1.863788e-62	-8.615850e-47
Msa0818310	Msa0970030	0.804066	2.581306e-49	-8.615850e-47
Msa0818310	Msa1004600	0.802079	6.650880e-49	-8.615850e-47
Msa0818310	Msa1006840	0.827506	1.506746e-54	-8.615850e-47
Msa0818310	Msa1012320	0.815496	8.975378e-52	-8.615850e-47
Msa0818310	Msa1013400	0.881237	2.835541e-70	-8.615850e-47
Msa0818310	Msa1019240	0.800789	1.222126e-48	-8.615850e-47
Msa0818310	Msa1019260	0.831311	1.793930e-55	-8.615850e-47
Msa0818310	Msa1024100	0.808379	3.188666e-50	-8.615850e-47
Msa0818310	Msa1040400	0.813351	2.675418e-51	-8.615850e-47
Msa0818310	Msa1066490	0.803885	2.815423e-49	-8.615850e-47
Msa0818310	Msa1078170	0.813592	2.368043e-51	-8.615850e-47
Msa0818310	Msa1078270	0.843545	1.311761e-58	-8.615850e-47
Msa0818310	Msa1081150	0.856176	3.839483e-62	-8.615850e-47
Msa0818310	Msa1081570	0.801228	9.940417e-49	-8.615850e-47
Msa0818310	Msa1081930	0.820721	5.904710e-53	-8.615850e-47
Msa0818310	Msa1081990	0.858717	6.801561e-63	-8.615850e-47
Msa0818310	Msa1083150	0.809569	1.773268e-50	-8.615850e-47
Msa0818310	Msa1091530	0.808058	3.732787e-50	-8.615850e-47
Msa0818310	Msa1093530	0.871490	6.567180e-67	-8.615850e-47
Msa0818310	Msa1093840	0.824802	6.625584e-54	-8.615850e-47
Msa0818310	Msa1101600	0.866024	3.856961e-65	-8.615850e-47
Msa0818310	Msa1104140	0.825466	4.617180e-54	-8.615850e-47
Msa0818310	Msa1119200	0.820307	7.350387e-53	-8.615850e-47
Msa0818310	Msa1123500	0.805146	1.537037e-49	-8.615850e-47
Msa0818310	Msa1125760	0.802327	5.914135e-49	-8.615850e-47
Msa0818310	Msa1125940	0.804857	1.766030e-49	-8.615850e-47
Msa0818310	Msa1126510	0.905733	3.141153e-80	-8.615850e-47
Msa0818310	Msa1126770	0.869906	2.179204e-66	-8.615850e-47
Msa0818310	Msa1126830	0.809425	1.904105e-50	-8.615850e-47
Msa0818310	Msa1127890	0.839740	1.322569e-57	-8.615850e-47
Msa0818310	Msa1128600	0.818641	1.763278e-52	-8.615850e-47
Msa0818310	Msa1130980	0.845961	2.928911e-59	-8.615850e-47
Msa0818310	Msa1134110	0.812258	4.641612e-51	-8.615850e-47
Msa0818310	Msa1158570	0.823605	1.265615e-53	-8.615850e-47
Msa0818310	Msa1165870	0.835189	1.939514e-56	-8.615850e-47
Msa0818310	Msa1165940	0.891658	3.249662e-74	-8.615850e-47
Msa0818310	Msa1166330	0.827331	1.659660e-54	-8.615850e-47
Msa0818310	Msa1166340	0.813561	2.404632e-51	-8.615850e-47
Msa0818310	Msa1166860	0.883646	3.762176e-71	-8.615850e-47
Msa0818310	Msa1169770	0.842777	2.101902e-58	-8.615850e-47
Msa0818310	Msa1171360	0.803316	3.695476e-49	-8.615850e-47
Msa0818310	Msa1184370	0.806687	7.286770e-50	-8.615850e-47
Msa0818310	Msa1187840	0.812078	5.081464e-51	-8.615850e-47
Msa0818310	Msa1196980	0.806113	9.630977e-50	-8.615850e-47
Msa0818310	Msa1207270	0.862127	6.312424e-64	-8.615850e-47
Msa0818310	Msa1210810	0.807278	5.465510e-50	-8.615850e-47
Msa0818310	Msa1218600	0.893490	5.987089e-75	-8.615850e-47
Msa0818310	Msa1221060	0.806268	8.935128e-50	-8.615850e-47
Msa0818310	Msa1227690	0.801394	9.194007e-49	-8.615850e-47
Msa0818310	Msa1229320	0.849902	2.400079e-60	-8.615850e-47
Msa0818310	Msa1239410	0.807978	3.880893e-50	-8.615850e-47
Msa0818310	Msa1239910	0.819627	1.051697e-52	-8.615850e-47
Msa0818310	Msa1251160	0.885221	9.800499e-72	-8.615850e-47
Msa0818310	Msa1254700	0.808148	3.571203e-50	-8.615850e-47
Msa0818310	Msa1257510	0.864890	8.776833e-65	-8.615850e-47
Msa0818310	Msa1283170	0.841397	4.871791e-58	-8.615850e-47
Msa0818310	Msa1292830	0.825762	3.928102e-54	-8.615850e-47
Msa0818310	Msa1303250	0.803693	3.085819e-49	-8.615850e-47
Msa0818310	Msa1305710	0.810527	1.102871e-50	-8.615850e-47
Msa0818310	Msa1307540	0.803644	3.159062e-49	-8.615850e-47
Msa0818310	Msa1307950	0.843939	1.028872e-58	-8.615850e-47
Msa0818310	Msa1321630	0.848229	7.002402e-60	-8.615850e-47
Msa0818310	Msa1343260	0.844636	6.687468e-59	-8.615850e-47
Msa0818310	Msa1353060	0.841662	4.146985e-58	-8.615850e-47
Msa0818310	Msa1356440	0.801764	7.720162e-49	-8.615850e-47
Msa0818310	Msa1358220	0.839794	1.280544e-57	-8.615850e-47
Msa0818310	Msa1361100	0.818153	2.273574e-52	-8.615850e-47
Msa0818310	Msa1361400	0.808473	3.044554e-50	-8.615850e-47
Msa0818310	Msa1361410	0.817229	3.674901e-52	-8.615850e-47
Msa0818310	Msa1363330	0.806015	1.010046e-49	-8.615850e-47
Msa0818310	Msa1366800	0.806804	6.884058e-50	-8.615850e-47
Msa0818310	Msa1367590	0.844580	6.921213e-59	-8.615850e-47
Msa0818310	Msa1367880	0.820934	5.275767e-53	-8.615850e-47
Msa0818310	Msa1370860	0.821035	5.000933e-53	-8.615850e-47
Msa0818310	Msa1374120	0.800294	1.541722e-48	-8.615850e-47
Msa0818310	Msa1374550	0.803271	3.775533e-49	-8.615850e-47
Msa0818310	Msa1376650	0.812821	3.496135e-51	-8.615850e-47
Msa0818310	Msa1379760	0.830380	3.033989e-55	-8.615850e-47
Msa0818310	Msa1382780	0.834228	3.384316e-56	-8.615850e-47
Msa0818310	Msa1386600	0.805770	1.136846e-49	-8.615850e-47
Msa0818310	Msa1394290	0.803721	3.045983e-49	-8.615850e-47
Msa0818310	Msa1404190	0.806629	7.495860e-50	-8.615850e-47
Msa0818310	Msa1407460	0.820864	5.474340e-53	-8.615850e-47
Msa0818310	Msa1413690	0.814959	1.180940e-51	-8.615850e-47
Msa0818310	Msa1418340	0.848664	5.307698e-60	-8.615850e-47
Msa0818310	Msa1420060	0.800871	1.176105e-48	-8.615850e-47
Msa0818310	Msa1421550	0.805610	1.228390e-49	-8.615850e-47
Msa0818310	Msa1430240	0.854927	8.883497e-62	-8.615850e-47
Msa0818310	Msa1432700	0.801405	9.145911e-49	-8.615850e-47
Msa0818310	Msa1436570	0.800046	1.731865e-48	-8.615850e-47
Msa0818310	Msa1443780	0.826077	3.307059e-54	-8.615850e-47
Msa0818310	Msa1446820	0.813744	2.192073e-51	-8.615850e-47
Msa0818310	Msa1448690	0.833613	4.822767e-56	-8.615850e-47
Msa0818310	Msa1450280	0.819899	9.114290e-53	-8.615850e-47
Msa0818310	Msa1451160	0.820154	7.967502e-53	-8.615850e-47
Msa0818310	Msa1456050	0.800581	1.347439e-48	-8.615850e-47
Msa0818310	Msa1457910	0.819997	8.656035e-53	-8.615850e-47
Msa0818310	Msa1465080	0.815468	9.102863e-52	-8.615850e-47
Msa0125450	Msa0818310	0.820855	5.501770e-53	-8.615850e-47
Msa0125540	Msa0818310	0.843113	1.710506e-58	-8.615850e-47
Msa0126370	Msa0818310	0.809010	2.337636e-50	-8.615850e-47
Msa0127310	Msa0818310	0.814041	1.885587e-51	-8.615850e-47
Msa0128860	Msa0818310	0.835278	1.841913e-56	-8.615850e-47
Msa0129060	Msa0818310	0.803091	4.112156e-49	-8.615850e-47
Msa0140820	Msa0818310	0.866030	3.839042e-65	-8.615850e-47
Msa0140850	Msa0818310	0.839065	1.979810e-57	-8.615850e-47
Msa0148060	Msa0818310	0.829329	5.472057e-55	-8.615850e-47
Msa0149190	Msa0818310	0.810924	9.049271e-51	-8.615850e-47
Msa0149200	Msa0818310	0.834444	2.986180e-56	-8.615850e-47
Msa0151370	Msa0818310	0.821981	3.023890e-53	-8.615850e-47
Msa0166590	Msa0818310	0.849354	3.411862e-60	-8.615850e-47
Msa0174730	Msa0818310	0.835798	1.360212e-56	-8.615850e-47
Msa0174770	Msa0818310	0.812545	4.017366e-51	-8.615850e-47
Msa0181610	Msa0818310	0.836880	7.219813e-57	-8.615850e-47
Msa0182620	Msa0818310	-0.819527	1.108144e-52	-8.615850e-47
Msa0186890	Msa0818310	0.807892	4.047401e-50	-8.615850e-47
Msa0187930	Msa0818310	0.823981	1.033395e-53	-8.615850e-47
Msa0189290	Msa0818310	0.813221	2.856444e-51	-8.615850e-47
Msa0189700	Msa0818310	0.839368	1.652655e-57	-8.615850e-47
Msa0194540	Msa0818310	0.824674	7.103798e-54	-8.615850e-47
Msa0197420	Msa0818310	0.807379	5.203191e-50	-8.615850e-47
Msa0197930	Msa0818310	0.828804	7.333844e-55	-8.615850e-47
Msa0199690	Msa0818310	0.800094	1.693139e-48	-8.615850e-47
Msa0199880	Msa0818310	0.837869	4.028783e-57	-8.615850e-47
Msa0223080	Msa0818310	0.812673	3.765686e-51	-8.615850e-47
Msa0229230	Msa0818310	0.828564	8.381885e-55	-8.615850e-47
Msa0229760	Msa0818310	0.877344	6.775781e-69	-8.615850e-47
Msa0231650	Msa0818310	0.819872	9.243497e-53	-8.615850e-47
Msa0232010	Msa0818310	0.854763	9.909563e-62	-8.615850e-47
Msa0232080	Msa0818310	0.855539	5.895112e-62	-8.615850e-47
Msa0366890	Msa0818310	0.820259	7.538413e-53	-8.615850e-47
Msa0369450	Msa0818310	0.900935	4.448788e-78	-8.615850e-47
Msa0376310	Msa0818310	0.811128	8.173513e-51	-8.615850e-47
Msa0380300	Msa0818310	0.856332	3.454895e-62	-8.615850e-47
Msa0387730	Msa0818310	0.867441	1.365609e-65	-8.615850e-47
Msa0390030	Msa0818310	0.821221	4.530031e-53	-8.615850e-47
Msa0392490	Msa0818310	0.804415	2.184042e-49	-8.615850e-47
Msa0395820	Msa0818310	0.843908	1.049054e-58	-8.615850e-47
Msa0405710	Msa0818310	0.800837	1.194939e-48	-8.615850e-47
Msa0427150	Msa0818310	0.810442	1.150560e-50	-8.615850e-47
Msa0432210	Msa0818310	0.841913	3.560997e-58	-8.615850e-47
Msa0438690	Msa0818310	0.821466	3.978564e-53	-8.615850e-47
Msa0439200	Msa0818310	0.814159	1.775525e-51	-8.615850e-47
Msa0468330	Msa0818310	0.834312	3.223347e-56	-8.615850e-47
Msa0471440	Msa0818310	0.802735	4.871271e-49	-8.615850e-47
Msa0475790	Msa0818310	0.809998	1.434009e-50	-8.615850e-47
Msa0482650	Msa0818310	0.828778	7.440177e-55	-8.615850e-47
Msa0239190	Msa0818310	0.812546	4.015188e-51	-8.615850e-47
Msa0239570	Msa0818310	0.818235	2.178531e-52	-8.615850e-47
Msa0242230	Msa0818310	0.810786	9.692519e-51	-8.615850e-47
Msa0242410	Msa0818310	0.809719	1.647023e-50	-8.615850e-47
Msa0244780	Msa0818310	0.823082	1.677191e-53	-8.615850e-47
Msa0255090	Msa0818310	0.826811	2.210695e-54	-8.615850e-47
Msa0259380	Msa0818310	0.820710	5.939305e-53	-8.615850e-47
Msa0268130	Msa0818310	0.833919	4.044613e-56	-8.615850e-47
Msa0270320	Msa0818310	0.802818	4.683867e-49	-8.615850e-47
Msa0273900	Msa0818310	0.816089	6.618337e-52	-8.615850e-47
Msa0277180	Msa0818310	0.808382	3.184128e-50	-8.615850e-47
Msa0280610	Msa0818310	0.886229	4.102320e-72	-8.615850e-47
Msa0292330	Msa0818310	0.809517	1.819509e-50	-8.615850e-47
Msa0294160	Msa0818310	0.812574	3.959972e-51	-8.615850e-47
Msa0298990	Msa0818310	0.835273	1.846981e-56	-8.615850e-47
Msa0300090	Msa0818310	0.847749	9.497944e-60	-8.615850e-47
Msa0300120	Msa0818310	0.830890	2.275724e-55	-8.615850e-47
Msa0302160	Msa0818310	0.817049	4.034165e-52	-8.615850e-47
Msa0315170	Msa0818310	0.854805	9.638381e-62	-8.615850e-47
Msa0316810	Msa0818310	0.809963	1.458972e-50	-8.615850e-47
Msa0317280	Msa0818310	0.841313	5.126975e-58	-8.615850e-47
Msa0317340	Msa0818310	0.850666	1.465559e-60	-8.615850e-47
Msa0339490	Msa0818310	0.814137	1.795640e-51	-8.615850e-47
Msa0487630	Msa0818310	0.810632	1.046786e-50	-8.615850e-47
Msa0497490	Msa0818310	0.807627	4.609739e-50	-8.615850e-47
Msa0518390	Msa0818310	0.890343	1.073639e-73	-8.615850e-47
Msa0518450	Msa0818310	0.889524	2.242180e-73	-8.615850e-47
Msa0521050	Msa0818310	0.873599	1.297546e-67	-8.615850e-47
Msa0523270	Msa0818310	0.803721	3.044640e-49	-8.615850e-47
Msa0524500	Msa0818310	0.855413	6.415033e-62	-8.615850e-47
Msa0526540	Msa0818310	0.801498	8.751594e-49	-8.615850e-47
Msa0527550	Msa0818310	0.855469	6.179079e-62	-8.615850e-47
Msa0541860	Msa0818310	0.810097	1.365290e-50	-8.615850e-47
Msa0552810	Msa0818310	0.872625	2.754169e-67	-8.615850e-47
Msa0556060	Msa0818310	0.809529	1.809340e-50	-8.615850e-47
Msa0566700	Msa0818310	0.850907	1.252947e-60	-8.615850e-47
Msa0575170	Msa0818310	0.821834	3.270011e-53	-8.615850e-47
Msa0581070	Msa0818310	0.852233	5.279537e-61	-8.615850e-47
Msa0581110	Msa0818310	0.815925	7.199499e-52	-8.615850e-47
Msa0585960	Msa0818310	0.808005	3.830549e-50	-8.615850e-47
Msa0588140	Msa0818310	0.805474	1.311815e-49	-8.615850e-47
Msa0588360	Msa0818310	0.823768	1.159469e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGATTGAATTTCCATTTC+AGG	0.174955	5_3:-53997886	Msa0818310:CDS
TATCCAACAAAATTATTAAT+AGG	0.224730	5_3:+53996658	None:intergenic
CGATGCAGTCTAGCAGAAAT+TGG	0.245631	5_3:-53996453	Msa0818310:three_prime_UTR
GATGATGGCTATACAGTTAA+AGG	0.253349	5_3:-53999870	None:intergenic
CATCTTAATCTCATTAGTTT+TGG	0.281168	5_3:-53996604	Msa0818310:three_prime_UTR
CCTTAGAATCTCGTTATATT+CGG	0.330694	5_3:-53996552	Msa0818310:three_prime_UTR
GGGAATGTCGGGTGTATGTT+GGG	0.332389	5_3:-53997923	Msa0818310:CDS
TAGATCTCTGTCTCATCTTT+CGG	0.343914	5_3:+53996755	None:intergenic
ACAATTGTCTTACCCTGAAA+TGG	0.370325	5_3:+53997873	None:intergenic
CCGAATATAACGAGATTCTA+AGG	0.379000	5_3:+53996552	None:intergenic
CAGATTGAATTTCCATTTCA+GGG	0.381938	5_3:-53997885	Msa0818310:intron
TTTGTCGTAAACCTAGTTGA+TGG	0.405515	5_3:+53996954	None:intergenic
TCGGGTGTATGTTGGGGAAC+TGG	0.428344	5_3:-53997916	Msa0818310:CDS
ATTTGGAGACAAGAGACAAA+TGG	0.431064	5_3:-53997944	Msa0818310:CDS
GAGATCTAAGTAGTGAGCTT+TGG	0.432068	5_3:-53996740	Msa0818310:exon
CCTGAAATGGAAATTCAATC+TGG	0.442021	5_3:+53997886	None:intergenic
AGTGGAAGAAGAAGCGTATG+AGG	0.483173	5_3:-53996795	Msa0818310:CDS
AAGAGACAAATGGGAATGTC+GGG	0.483284	5_3:-53997934	Msa0818310:CDS
TTTGGAGACAAGAGACAAAT+GGG	0.483437	5_3:-53997943	Msa0818310:CDS
TGGGAATGTCGGGTGTATGT+TGG	0.485092	5_3:-53997924	Msa0818310:CDS
TCTTTCAGCGGCCATCAACT+AGG	0.508309	5_3:-53996965	Msa0818310:intron
GATGCAGTCTAGCAGAAATT+GGG	0.521054	5_3:-53996452	Msa0818310:three_prime_UTR
CAAGAGACAAATGGGAATGT+CGG	0.521145	5_3:-53997935	Msa0818310:CDS
ACCTTGGCTCTCATCTTGAA+CGG	0.524585	5_3:+53996923	None:intergenic
AGCGTATGAGGAGATTGAAG+AGG	0.544618	5_3:-53996783	Msa0818310:CDS
GGAATGTCGGGTGTATGTTG+GGG	0.631965	5_3:-53997922	Msa0818310:CDS
ACCGTTCAAGATGAGAGCCA+AGG	0.659345	5_3:-53996924	Msa0818310:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTATAAAGAAATTTAATCA+AGG	-	chr5_3:53997359-53997378	Msa0818310:intron	10.0%
!!!	TTAATAATAGTGAATTATTT+TGG	-	chr5_3:53997883-53997902	Msa0818310:intron	10.0%
!!!	ATTAGAAATTTTTAGATTTA+GGG	+	chr5_3:53998833-53998852	None:intergenic	10.0%
!!!	AATTAGAAATTTTTAGATTT+AGG	+	chr5_3:53998834-53998853	None:intergenic	10.0%
!!	ATATAACATATAAACATAAA+AGG	-	chr5_3:53998865-53998884	Msa0818310:intron	10.0%
!!	AAACCAAAAAAATAAGTTAA+GGG	-	chr5_3:53996746-53996765	Msa0818310:exon	15.0%
!!	GAATCCAAAAAATAAATTAA+GGG	-	chr5_3:53996866-53996885	Msa0818310:intron	15.0%
!!	ACATTATAAATAGTAATCTT+TGG	-	chr5_3:53997051-53997070	Msa0818310:intron	15.0%
!!	AATTATTTAGAAGAATGTTA+TGG	+	chr5_3:53997340-53997359	None:intergenic	15.0%
!!!	ACAAAACTTTTAATAGTTAA+GGG	+	chr5_3:53997434-53997453	None:intergenic	15.0%
!!!	AACAAAACTTTTAATAGTTA+AGG	+	chr5_3:53997435-53997454	None:intergenic	15.0%
!!!	AACTATTAAAAGTTTTGTTT+TGG	-	chr5_3:53997436-53997455	Msa0818310:intron	15.0%
!!!	CATATCGTTTTATATAATTT+TGG	-	chr5_3:53997810-53997829	Msa0818310:intron	15.0%
!!!	GATTTTGTAAAACTATTTTT+AGG	-	chr5_3:53998336-53998355	Msa0818310:intron	15.0%
!!!	ATTTTGATTTTTTTAGATGA+AGG	+	chr5_3:53998665-53998684	None:intergenic	15.0%
!!!	AGAGAATTTTTTTAGATTTA+GGG	+	chr5_3:53998697-53998716	None:intergenic	15.0%
!!!	TAGAGAATTTTTTTAGATTT+AGG	+	chr5_3:53998698-53998717	None:intergenic	15.0%
!!!	TGTTTAAATTTTGGTTTTTT+TGG	+	chr5_3:53998808-53998827	None:intergenic	15.0%
!!	ATATAAACATAAAAGGTTAA+TGG	-	chr5_3:53998872-53998891	Msa0818310:intron	15.0%
!!!	TTCTGATTTTATCTTAATTT+TGG	-	chr5_3:53999460-53999479	Msa0818310:intron	15.0%
!!!	AATCCTATTAATAATTTTGT+TGG	-	chr5_3:53999650-53999669	Msa0818310:intron	15.0%
!!	TATCCAACAAAATTATTAAT+AGG	+	chr5_3:53999656-53999675	None:intergenic	15.0%
!!!	AACTACTTTGATAATTTTTT+TGG	-	chr5_3:53999799-53999818	Msa0818310:intron	15.0%
!!!	ACTACTTTGATAATTTTTTT+GGG	-	chr5_3:53999800-53999819	Msa0818310:intron	15.0%
!!	GAAACCAAAAAATAAGTTAA+GGG	+	chr5_3:53996714-53996733	None:intergenic	20.0%
!!!	ACGAAACTTTTAATAGTTAA+GGG	+	chr5_3:53996775-53996794	None:intergenic	20.0%
!!!	AACGAAACTTTTAATAGTTA+AGG	+	chr5_3:53996774-53996793	None:intergenic	20.0%
!!!	ACGAAACTTTTAATAGTTAA+GGG	+	chr5_3:53996775-53996794	None:intergenic	20.0%
!!!	AACGAAACTTTTAATAGTTA+AGG	+	chr5_3:53996774-53996793	None:intergenic	20.0%
!!!	AACTATTAAAAGTTTCGTTT+TGG	-	chr5_3:53996522-53996541	Msa0818310:three_prime_UTR	20.0%
!!	GAAACCAAAAAATAAGTTAA+GGG	+	chr5_3:53996714-53996733	None:intergenic	20.0%
!!	GAAACCAAAAAATAAGTTAA+GGG	-	chr5_3:53996714-53996733	Msa0818310:three_prime_UTR	20.0%
!!	GAAACCAAAAAAATAAGTTA+AGG	-	chr5_3:53996745-53996764	Msa0818310:exon	20.0%
!!!	AACTATTAAAAGTTTCGTTT+TGG	+	chr5_3:53996522-53996541	None:intergenic	20.0%
!!!	AACGAAACTTTTAATAGTTA+AGG	-	chr5_3:53996774-53996793	Msa0818310:CDS	20.0%
!!!	ACGAAACTTTTAATAGTTAA+GGG	-	chr5_3:53996775-53996794	Msa0818310:CDS	20.0%
!!	AAAACCCAAAAATAAGTTAA+GGG	-	chr5_3:53996806-53996825	Msa0818310:intron	20.0%
!!	AACTATTAAAAGTTTCGTTT+CGG	+	chr5_3:53996522-53996541	None:intergenic	20.0%
!!!	TTTATTTTTTGGATTCGTTT+TGG	+	chr5_3:53996862-53996881	None:intergenic	20.0%
!!	CGAATCCAAAAAATAAATTA+AGG	-	chr5_3:53996865-53996884	Msa0818310:intron	20.0%
!!!	AGACCAAGAAAAATAATTTT+TGG	-	chr5_3:53997027-53997046	Msa0818310:intron	20.0%
!!	GAAACCAAAAAATAAGTTAA+GGG	+	chr5_3:53996714-53996733	None:intergenic	20.0%
!!	TAAACCAAAAACTAAGTTAA+GGG	+	chr5_3:53997463-53997482	None:intergenic	20.0%
!!!	ACGAAACTTTTAATAGTTAA+GGG	+	chr5_3:53996775-53996794	None:intergenic	20.0%
!!!	AACGAAACTTTTAATAGTTA+AGG	+	chr5_3:53996774-53996793	None:intergenic	20.0%
!!!	AACTATTAAAAGTTTCGTTT+TGG	-	chr5_3:53996522-53996541	Msa0818310:three_prime_UTR	20.0%
!!	GAAACCAAAAAATAAGTTAA+GGG	+	chr5_3:53996714-53996733	None:intergenic	20.0%
!!	GAAACCAAAAAATAAGTTAA+GGG	-	chr5_3:53996714-53996733	Msa0818310:three_prime_UTR	20.0%
!!!	AACTATTAAAAGTTTCGTTT+TGG	+	chr5_3:53996522-53996541	None:intergenic	20.0%
!!!	AACGAAACTTTTAATAGTTA+AGG	-	chr5_3:53996774-53996793	Msa0818310:CDS	20.0%
!!!	ACGAAACTTTTAATAGTTAA+GGG	-	chr5_3:53996775-53996794	Msa0818310:CDS	20.0%
!!	AAAACCCAAAAATAAGTTAA+GGG	-	chr5_3:53996806-53996825	Msa0818310:intron	20.0%
!!	AACTATTAAAAGTTTCGTTT+CGG	+	chr5_3:53996522-53996541	None:intergenic	20.0%
!!	GAAACCAACAAATAAATTAA+GGG	-	chr5_3:53997746-53997765	Msa0818310:intron	20.0%
!!	GTTTGATATGCTAAAAAATA+AGG	-	chr5_3:53998276-53998295	Msa0818310:intron	20.0%
!!!	ATTTTTAGGATAAGACAATT+TGG	-	chr5_3:53998350-53998369	Msa0818310:intron	20.0%
!!!	CAAGAATTTAATCTTTTGAA+TGG	+	chr5_3:53998574-53998593	None:intergenic	20.0%
!!	CAAAAGATTAAATTCTTGTT+AGG	-	chr5_3:53998576-53998595	Msa0818310:intron	20.0%
!!!	TTTAGGGTTTGTTTAAATTT+TGG	+	chr5_3:53998681-53998700	None:intergenic	20.0%
!!!	TATGAAAACTTACTTGTTAT+TGG	-	chr5_3:53998903-53998922	Msa0818310:intron	20.0%
!!	CTTAATTCTTGTTCAAATTT+GGG	-	chr5_3:53999191-53999210	Msa0818310:intron	20.0%
!!!	TTTCATGTTTTGATACATTA+TGG	-	chr5_3:53999678-53999697	Msa0818310:intron	20.0%
!!!	TTCATGTTTTGATACATTAT+GGG	-	chr5_3:53999679-53999698	Msa0818310:intron	20.0%
!	CGAAACCAAAAAATAAGTTA+AGG	+	chr5_3:53996713-53996732	None:intergenic	25.0%
!!!	CTTATTTTTTGGTTTCGTTT+TGG	-	chr5_3:53996493-53996512	Msa0818310:three_prime_UTR	25.0%
!	GAAACCAAAAAGTAAGTTAA+GGG	+	chr5_3:53996549-53996568	None:intergenic	25.0%
!	CGAAACCAAAAAATAAGTTA+AGG	+	chr5_3:53996713-53996732	None:intergenic	25.0%
!!!	CTTATTTTTTGGTTTCGTTT+TGG	-	chr5_3:53996493-53996512	Msa0818310:three_prime_UTR	25.0%
!	ATACATTCATCCTAATGTAA+AGG	+	chr5_3:53996674-53996693	None:intergenic	25.0%
!	GATGAATGTATTAGAGTTAA+GGG	-	chr5_3:53996683-53996702	Msa0818310:three_prime_UTR	25.0%
!!!	CTTATTTTTTGGTTTCGTTT+TGG	+	chr5_3:53996493-53996512	None:intergenic	25.0%
!	CGAAACCAAAAAATAAGTTA+AGG	-	chr5_3:53996713-53996732	Msa0818310:three_prime_UTR	25.0%
!!!	TAGTCCCTTAACTTATTTTT+TGG	+	chr5_3:53996721-53996740	None:intergenic	25.0%
!!!	CTTATTTTTGGGTTTTCTTT+TGG	+	chr5_3:53996802-53996821	None:intergenic	25.0%
!	GAAAACCCAAAAATAAGTTA+AGG	-	chr5_3:53996805-53996824	Msa0818310:intron	25.0%
!!!	TGGTCCCTTAATTTATTTTT+TGG	+	chr5_3:53996873-53996892	None:intergenic	25.0%
!	GTCTGAGAAAATATCAATAT+TGG	+	chr5_3:53996938-53996957	None:intergenic	25.0%
!!!	GTGCCAAAAATTATTTTTCT+TGG	+	chr5_3:53997033-53997052	None:intergenic	25.0%
!	CGAAACCAAAAAATAAGTTA+AGG	+	chr5_3:53996713-53996732	None:intergenic	25.0%
!!!	CTTATTTTTTGGTTTCGTTT+TGG	-	chr5_3:53996493-53996512	Msa0818310:three_prime_UTR	25.0%
!	CTAAACCAAAAACTAAGTTA+AGG	+	chr5_3:53997464-53997483	None:intergenic	25.0%
!!!	CTTAGTTTTTGGTTTAGTTT+TGG	-	chr5_3:53997467-53997486	Msa0818310:intron	25.0%
!	CGAAACCAAAAAATAAGTTA+AGG	+	chr5_3:53996713-53996732	None:intergenic	25.0%
!!!	CTTATTTTTTGGTTTCGTTT+TGG	-	chr5_3:53996493-53996512	Msa0818310:three_prime_UTR	25.0%
!	ATACATTCATCCTAACATAA+AGG	+	chr5_3:53997586-53997605	None:intergenic	25.0%
!	GATGAATGTATTAGAGTTAA+GGG	-	chr5_3:53996683-53996702	Msa0818310:three_prime_UTR	25.0%
!!!	CTTATTTTTTGGTTTCGTTT+TGG	+	chr5_3:53996493-53996512	None:intergenic	25.0%
!	CGAAACCAAAAAATAAGTTA+AGG	-	chr5_3:53996713-53996732	Msa0818310:three_prime_UTR	25.0%
!!!	CTTATTTTTGGGTTTTCTTT+TGG	+	chr5_3:53996802-53996821	None:intergenic	25.0%
!	GAAAACCCAAAAATAAGTTA+AGG	-	chr5_3:53996805-53996824	Msa0818310:intron	25.0%
!!!	TTTATTTGTTGGTTTCGTTT+TGG	+	chr5_3:53997742-53997761	None:intergenic	25.0%
!	CGAAACCAACAAATAAATTA+AGG	-	chr5_3:53997745-53997764	Msa0818310:intron	25.0%
!!!	CATTGGCTTAATTACTTTTT+TGG	+	chr5_3:53997773-53997792	None:intergenic	25.0%
!!	CCTTTTATCTTTATCTGTTT+GGG	+	chr5_3:53998046-53998065	None:intergenic	25.0%
!!	TCCTTTTATCTTTATCTGTT+TGG	+	chr5_3:53998047-53998066	None:intergenic	25.0%
!!	TTGTCTTTTGCATTTTTAAC+TGG	-	chr5_3:53998218-53998237	Msa0818310:intron	25.0%
!	GATATGCTAAAAAATAAGGA+CGG	-	chr5_3:53998280-53998299	Msa0818310:intron	25.0%
!!	TTTGAATTTGCTCTATTCAA+AGG	+	chr5_3:53998504-53998523	None:intergenic	25.0%
!!	TAATCTTTTGAATGGAACAA+TGG	+	chr5_3:53998566-53998585	None:intergenic	25.0%
!	GATTAAATTCTTGTTAGGTT+TGG	-	chr5_3:53998581-53998600	Msa0818310:intron	25.0%
!!	GTTAGGTTTGGTTTAAATAA+CGG	-	chr5_3:53998593-53998612	Msa0818310:intron	25.0%
!!	ATTTGAACTTGAAGGAATTT+TGG	-	chr5_3:53998937-53998956	Msa0818310:intron	25.0%
!	AGATGAAAAATGAGAAAATG+AGG	-	chr5_3:53999004-53999023	Msa0818310:intron	25.0%
!!	AACTAATGCAAGAACATTTT+TGG	-	chr5_3:53999128-53999147	Msa0818310:intron	25.0%
!	GCTTAATTCTTGTTCAAATT+TGG	-	chr5_3:53999190-53999209	Msa0818310:intron	25.0%
!!!	CATCTTAATCTCATTAGTTT+TGG	-	chr5_3:53999707-53999726	Msa0818310:intron	25.0%
!	GGTGCTTATAATTACTCTTT+TGG	-	chr5_3:53996462-53996481	Msa0818310:three_prime_UTR	30.0%
!!!	TGGTCCCTTAACTTATTTTT+TGG	-	chr5_3:53996482-53996501	Msa0818310:three_prime_UTR	30.0%
	CGAAACCAAAAAGTAAGTTA+AGG	+	chr5_3:53996550-53996569	None:intergenic	30.0%
!!!	TACTTTTTGGTTTCGTGTTT+TGG	-	chr5_3:53996555-53996574	Msa0818310:three_prime_UTR	30.0%
!!!	TGGTCCCTTAACTTATTTTT+TGG	-	chr5_3:53996482-53996501	Msa0818310:three_prime_UTR	30.0%
	GGATGAATGTATTAGAGTTA+AGG	-	chr5_3:53996682-53996701	Msa0818310:three_prime_UTR	30.0%
!!!	GGTCCCTTAACTTATTTTTT+TGG	+	chr5_3:53996483-53996502	None:intergenic	30.0%
	GACACTATAGATGCATTAAA+AGG	+	chr5_3:53997126-53997145	None:intergenic	30.0%
!	TCAAGGCTTAATTACTCTTT+TGG	-	chr5_3:53997376-53997395	Msa0818310:intron	30.0%
!!!	TGGTCCCTTAACTTATTTTT+TGG	-	chr5_3:53996482-53996501	Msa0818310:three_prime_UTR	30.0%
!!!	TGGTCCCTTAACTTATTTTT+TGG	-	chr5_3:53996482-53996501	Msa0818310:three_prime_UTR	30.0%
	GGATGAATGTATTAGAGTTA+AGG	-	chr5_3:53996682-53996701	Msa0818310:three_prime_UTR	30.0%
!!!	TGGTCCCTTAACTTATTTTT+TGG	+	chr5_3:53996482-53996501	None:intergenic	30.0%
	TGGTCCCTTAATTTATTTGT+TGG	+	chr5_3:53997753-53997772	None:intergenic	30.0%
	TCTTTGTATGTGTTTCTCAT+TGG	+	chr5_3:53997790-53997809	None:intergenic	30.0%
	CGTCTTAGATTTACATTTCT+CGG	+	chr5_3:53997849-53997868	None:intergenic	30.0%
	CCACAAACTTCAAACTTAAT+AGG	+	chr5_3:53997924-53997943	None:intergenic	30.0%
!	CCTATTAAGTTTGAAGTTTG+TGG	-	chr5_3:53997921-53997940	Msa0818310:CDS	30.0%
	ACAAAATGAAAGCAGCAATA+TGG	+	chr5_3:53997948-53997967	None:intergenic	30.0%
!!!	TTGCTGCTTTCATTTTGTTT+TGG	-	chr5_3:53997950-53997969	Msa0818310:CDS	30.0%
	TAAGAACACTAGAGATCAAA+AGG	-	chr5_3:53997997-53998016	Msa0818310:intron	30.0%
	AAGAACACTAGAGATCAAAA+GGG	-	chr5_3:53997998-53998017	Msa0818310:intron	30.0%
	CCCAAACAGATAAAGATAAA+AGG	-	chr5_3:53998043-53998062	Msa0818310:intron	30.0%
	GTCACATGAAGATAAATACA+TGG	+	chr5_3:53998086-53998105	None:intergenic	30.0%
	TGAAACTTCATGTTCCAATT+AGG	-	chr5_3:53998159-53998178	Msa0818310:intron	30.0%
	TTTCAAACTCATAGCCTAAT+TGG	+	chr5_3:53998176-53998195	None:intergenic	30.0%
	ATTAGGCTATGAGTTTGAAA+AGG	-	chr5_3:53998176-53998195	Msa0818310:intron	30.0%
	TTAGGCTATGAGTTTGAAAA+GGG	-	chr5_3:53998177-53998196	Msa0818310:intron	30.0%
	CAGATTGAATTTCCATTTCA+GGG	-	chr5_3:53998426-53998445	Msa0818310:intron	30.0%
	GGTATGATAATTGTTGTAGT+TGG	+	chr5_3:53998483-53998502	None:intergenic	30.0%
	ATTCAAGTGTCAAAAGAATG+AGG	-	chr5_3:53998617-53998636	Msa0818310:intron	30.0%
!!	GTTGTTTGATTTGAACTTGA+AGG	-	chr5_3:53998929-53998948	Msa0818310:intron	30.0%
!!!	ACTTGAAGGAATTTTGGTTT+TGG	-	chr5_3:53998943-53998962	Msa0818310:intron	30.0%
!!!	CTTGAAGGAATTTTGGTTTT+GGG	-	chr5_3:53998944-53998963	Msa0818310:intron	30.0%
!	TTGGACCTATTTTCTGTTTT+TGG	+	chr5_3:53999244-53999263	None:intergenic	30.0%
!	GTAAATGGGCTTTAATCAAT+TGG	+	chr5_3:53999263-53999282	None:intergenic	30.0%
	CAGATAATAAGCACGTAAAT+GGG	+	chr5_3:53999277-53999296	None:intergenic	30.0%
!!	TTGTCTTTTTCATCTTTCAG+CGG	-	chr5_3:53999334-53999353	Msa0818310:intron	30.0%
!!	CTATTGTTTGCTTTTATCAG+TGG	-	chr5_3:53999498-53999517	Msa0818310:intron	30.0%
	CCTTAGAATCTCGTTATATT+CGG	-	chr5_3:53999759-53999778	Msa0818310:intron	30.0%
!!	TGCTCCCTTAACTTACTTTT+TGG	-	chr5_3:53996542-53996561	Msa0818310:three_prime_UTR	35.0%
!!	CGGTCCCTTAACTTATTTTT+GGG	+	chr5_3:53996813-53996832	None:intergenic	35.0%
!!	TCGGTCCCTTAACTTATTTT+TGG	+	chr5_3:53996814-53996833	None:intergenic	35.0%
	CCAAAAGAGTAATTAAGCCT+CGG	-	chr5_3:53996890-53996909	Msa0818310:intron	35.0%
	CAATATTGGAATGCTCAACT+TGG	+	chr5_3:53996924-53996943	None:intergenic	35.0%
	CAGGCTAAGATACTTATAGT+GGG	+	chr5_3:53996981-53997000	None:intergenic	35.0%
	TATAAGTATCTTAGCCTGCA+TGG	-	chr5_3:53996983-53997002	Msa0818310:intron	35.0%
!!!	GGTTTTTTAAACGTGTGAGA+CGG	-	chr5_3:53997072-53997091	Msa0818310:intron	35.0%
!!	ATAAAAGAGTGAGTGTTGGA+AGG	+	chr5_3:53997198-53997217	None:intergenic	35.0%
!	TCCAACACTCACTCTTTTAT+TGG	-	chr5_3:53997198-53997217	Msa0818310:intron	35.0%
!!	TGGTCCCTTAACTTAGTTTT+TGG	-	chr5_3:53997456-53997475	Msa0818310:intron	35.0%
!!	CGGTCCCTTAACTTATTTTT+GGG	+	chr5_3:53996813-53996832	None:intergenic	35.0%
!!	TCGGTCCCTTAACTTATTTT+TGG	+	chr5_3:53996814-53996833	None:intergenic	35.0%
	AGAACACTAGAGATCAAAAG+GGG	-	chr5_3:53997999-53998018	Msa0818310:intron	35.0%
	TCAAAAGGGGGATATGTTAA+TGG	-	chr5_3:53998012-53998031	Msa0818310:intron	35.0%
	TAGGCTATGAGTTTGAAAAG+GGG	-	chr5_3:53998178-53998197	Msa0818310:intron	35.0%
	GAAAAGGGGGAATATTCTTA+CGG	-	chr5_3:53998192-53998211	Msa0818310:intron	35.0%
	ATTTGGAGACAAGAGACAAA+TGG	-	chr5_3:53998367-53998386	Msa0818310:intron	35.0%
	TTTGGAGACAAGAGACAAAT+GGG	-	chr5_3:53998368-53998387	Msa0818310:intron	35.0%
	CCTGAAATGGAAATTCAATC+TGG	+	chr5_3:53998428-53998447	None:intergenic	35.0%
	CCAGATTGAATTTCCATTTC+AGG	-	chr5_3:53998425-53998444	Msa0818310:intron	35.0%
	ACAATTGTCTTACCCTGAAA+TGG	+	chr5_3:53998441-53998460	None:intergenic	35.0%
	GGTAAGACAATTGTTGTAGT+TGG	-	chr5_3:53998447-53998466	Msa0818310:intron	35.0%
!	GAATGGAACAATGGTAATGT+CGG	+	chr5_3:53998557-53998576	None:intergenic	35.0%
!!	ATGAAGGTATTGAAGTGTCA+AGG	+	chr5_3:53998649-53998668	None:intergenic	35.0%
!!!	ATGCAAGAACATTTTTGGCA+TGG	-	chr5_3:53999133-53999152	Msa0818310:intron	35.0%
!!!	ACCTATTTTCTGTTTTTGGC+GGG	+	chr5_3:53999240-53999259	None:intergenic	35.0%
!!!	GACCTATTTTCTGTTTTTGG+CGG	+	chr5_3:53999241-53999260	None:intergenic	35.0%
	GCAGATAATAAGCACGTAAA+TGG	+	chr5_3:53999278-53999297	None:intergenic	35.0%
	TTACGTGCTTATTATCTGCT+AGG	-	chr5_3:53999279-53999298	Msa0818310:intron	35.0%
	TACGTGCTTATTATCTGCTA+GGG	-	chr5_3:53999280-53999299	Msa0818310:intron	35.0%
	CAAGGTTGCAACGATTTATA+GGG	+	chr5_3:53999317-53999336	None:intergenic	35.0%
	ACAAGGTTGCAACGATTTAT+AGG	+	chr5_3:53999318-53999337	None:intergenic	35.0%
	GCTGAAAGATGAAAAAGACA+AGG	+	chr5_3:53999335-53999354	None:intergenic	35.0%
	TTTGTCGTAAACCTAGTTGA+TGG	+	chr5_3:53999360-53999379	None:intergenic	35.0%
	AGCAGTGAAATAGTTTACCT+TGG	+	chr5_3:53999407-53999426	None:intergenic	35.0%
	TAGATCTCTGTCTCATCTTT+CGG	+	chr5_3:53999559-53999578	None:intergenic	35.0%
	CCGAATATAACGAGATTCTA+AGG	+	chr5_3:53999762-53999781	None:intergenic	35.0%
	TAAAGGACCAAAGTGGTTGA+CGG	+	chr5_3:53996657-53996676	None:intergenic	40.0%
	CCTAATGTAAAGGACCAAAG+TGG	+	chr5_3:53996664-53996683	None:intergenic	40.0%
	CCACTTTGGTCCTTTACATT+AGG	-	chr5_3:53996661-53996680	Msa0818310:three_prime_UTR	40.0%
!	CCGAGGCTTAATTACTCTTT+TGG	+	chr5_3:53996893-53996912	None:intergenic	40.0%
	TCAACTTGGATGTAAATCCG+AGG	+	chr5_3:53996910-53996929	None:intergenic	40.0%
	GCAGGCTAAGATACTTATAG+TGG	+	chr5_3:53996982-53997001	None:intergenic	40.0%
!	TAGCCTGCATGGAGAATTTT+TGG	-	chr5_3:53996994-53997013	Msa0818310:intron	40.0%
	GCCAATAAAAGAGTGAGTGT+TGG	+	chr5_3:53997202-53997221	None:intergenic	40.0%
!!	TTTTATTGGCCTACATCACG+TGG	-	chr5_3:53997212-53997231	Msa0818310:intron	40.0%
!	TTTATTGGCCTACATCACGT+GGG	-	chr5_3:53997213-53997232	Msa0818310:intron	40.0%
	TGGGTCTCACATATTGGAAA+TGG	-	chr5_3:53997232-53997251	Msa0818310:intron	40.0%
	GGGTCTCACATATTGGAAAT+GGG	-	chr5_3:53997233-53997252	Msa0818310:intron	40.0%
	TAAAGGACCAAAGTGGTTGA+CGG	+	chr5_3:53996657-53996676	None:intergenic	40.0%
	CCTAACATAAAGGACCAAAG+TGG	+	chr5_3:53997576-53997595	None:intergenic	40.0%
	CCACTTTGGTCCTTTATGTT+AGG	-	chr5_3:53997573-53997592	Msa0818310:intron	40.0%
	GAACACTAGAGATCAAAAGG+GGG	-	chr5_3:53998000-53998019	Msa0818310:intron	40.0%
	AGGCTATGAGTTTGAAAAGG+GGG	-	chr5_3:53998179-53998198	Msa0818310:intron	40.0%
	CAAGAGACAAATGGGAATGT+CGG	-	chr5_3:53998376-53998395	Msa0818310:intron	40.0%
	AAGAGACAAATGGGAATGTC+GGG	-	chr5_3:53998377-53998396	Msa0818310:intron	40.0%
	ATTCAAACTTGCCAAACAGC+AGG	-	chr5_3:53998517-53998536	Msa0818310:intron	40.0%
!	GGTATTGAAGTGTCAAGGAA+TGG	+	chr5_3:53998644-53998663	None:intergenic	40.0%
	ACTCACGCTTGTCTTAAACA+AGG	+	chr5_3:53999037-53999056	None:intergenic	40.0%
	GAGATCTAAGTAGTGAGCTT+TGG	-	chr5_3:53999571-53999590	Msa0818310:intron	40.0%
	GATGCAGTCTAGCAGAAATT+GGG	-	chr5_3:53999859-53999878	Msa0818310:intron	40.0%
	GTTGACGGAGGAAGAGTTAA+GGG	+	chr5_3:53996642-53996661	None:intergenic	45.0%
	GTGCCAAAAATTCTCCATGC+AGG	+	chr5_3:53997000-53997019	None:intergenic	45.0%
	ATCACGTGGGTCTCACATAT+TGG	-	chr5_3:53997226-53997245	Msa0818310:intron	45.0%
	GTTGACGGAGGAAGAGTTAA+GGG	+	chr5_3:53996642-53996661	None:intergenic	45.0%
	AATGTTGGAGTCCTGCTGTT+TGG	+	chr5_3:53998531-53998550	None:intergenic	45.0%
!	TGGTAATGTCGGTCGAATGT+TGG	+	chr5_3:53998546-53998565	None:intergenic	45.0%
!!!	GGTTTTGGGTGATTTGAGAG+AGG	-	chr5_3:53998958-53998977	Msa0818310:intron	45.0%
	GCGTGAGTTAACTAACGATG+CGG	-	chr5_3:53999049-53999068	Msa0818310:intron	45.0%
!!!	AACATTTTTGGCATGGCTGC+AGG	-	chr5_3:53999140-53999159	Msa0818310:intron	45.0%
!!!	ACATTTTTGGCATGGCTGCA+GGG	-	chr5_3:53999141-53999160	Msa0818310:intron	45.0%
!!!	TGGCGGGCTTCTAAGTTTTT+GGG	+	chr5_3:53999224-53999243	None:intergenic	45.0%
!!!	TTGGCGGGCTTCTAAGTTTT+TGG	+	chr5_3:53999225-53999244	None:intergenic	45.0%
	GCCCGCCAAAAACAGAAAAT+AGG	-	chr5_3:53999236-53999255	Msa0818310:intron	45.0%
!	ACCTTGGCTCTCATCTTGAA+CGG	+	chr5_3:53999391-53999410	None:intergenic	45.0%
!	AGTGGAAGAAGAAGCGTATG+AGG	-	chr5_3:53999516-53999535	Msa0818310:intron	45.0%
	AGCGTATGAGGAGATTGAAG+AGG	-	chr5_3:53999528-53999547	Msa0818310:intron	45.0%
	CGATGCAGTCTAGCAGAAAT+TGG	-	chr5_3:53999858-53999877	Msa0818310:intron	45.0%
	GGTTGACGGAGGAAGAGTTA+AGG	+	chr5_3:53996643-53996662	None:intergenic	50.0%
	TCTTCCTCCGTCAACCACTT+TGG	-	chr5_3:53996647-53996666	Msa0818310:three_prime_UTR	50.0%
	ATGTGAGACCCACGTGATGT+AGG	+	chr5_3:53997224-53997243	None:intergenic	50.0%
	GGTTGACGGAGGAAGAGTTA+AGG	+	chr5_3:53996643-53996662	None:intergenic	50.0%
	TCTTCCTCCGTCAACCACTT+TGG	-	chr5_3:53996647-53996666	Msa0818310:three_prime_UTR	50.0%
	TGGGAATGTCGGGTGTATGT+TGG	-	chr5_3:53998387-53998406	Msa0818310:intron	50.0%
	GGGAATGTCGGGTGTATGTT+GGG	-	chr5_3:53998388-53998407	Msa0818310:intron	50.0%
	GGAATGTCGGGTGTATGTTG+GGG	-	chr5_3:53998389-53998408	Msa0818310:intron	50.0%
	TCTTTCAGCGGCCATCAACT+AGG	-	chr5_3:53999346-53999365	Msa0818310:intron	50.0%
	ACCGTTCAAGATGAGAGCCA+AGG	-	chr5_3:53999387-53999406	Msa0818310:intron	50.0%
	AGGACCAAAGTGGTTGACGG+AGG	+	chr5_3:53996654-53996673	None:intergenic	55.0%
	AGGACCAAAGTGGTTGACGG+AGG	+	chr5_3:53996654-53996673	None:intergenic	55.0%
	TCGGGTGTATGTTGGGGAAC+TGG	-	chr5_3:53998395-53998414	Msa0818310:intron	55.0%
!	AGGGCGCGAGCGAAAAGTGC+AGG	-	chr5_3:53999160-53999179	Msa0818310:intron	65.0%
!	GGGCGCGAGCGAAAAGTGCA+GGG	-	chr5_3:53999161-53999180	Msa0818310:intron	65.0%
!	GGCGCGAGCGAAAAGTGCAG+GGG	-	chr5_3:53999162-53999181	Msa0818310:intron	65.0%
!	GCGCGAGCGAAAAGTGCAGG+GGG	-	chr5_3:53999163-53999182	Msa0818310:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	53996445	53999888	53996445	ID=Msa0818310;Name=Msa0818310
chr5_3	mRNA	53996445	53999888	53996445	ID=Msa0818310-mRNA-1;Parent=Msa0818310;Name=Msa0818310-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|0.33\|0.25\|0.5\|1\|1\|4\|309\|74
chr5_3	exon	53996445	53996815	53996445	ID=Msa0818310-mRNA-1:exon:16374;Parent=Msa0818310-mRNA-1
chr5_3	exon	53996925	53996979	53996925	ID=Msa0818310-mRNA-1:exon:16373;Parent=Msa0818310-mRNA-1
chr5_3	exon	53997886	53997975	53997886	ID=Msa0818310-mRNA-1:exon:16372;Parent=Msa0818310-mRNA-1
chr5_3	exon	53999871	53999888	53999871	ID=Msa0818310-mRNA-1:exon:16371;Parent=Msa0818310-mRNA-1
chr5_3	CDS	53999871	53999888	53999871	ID=Msa0818310-mRNA-1:cds;Parent=Msa0818310-mRNA-1
chr5_3	CDS	53997886	53997975	53997886	ID=Msa0818310-mRNA-1:cds;Parent=Msa0818310-mRNA-1
chr5_3	CDS	53996925	53996979	53996925	ID=Msa0818310-mRNA-1:cds;Parent=Msa0818310-mRNA-1
chr5_3	CDS	53996754	53996815	53996754	ID=Msa0818310-mRNA-1:cds;Parent=Msa0818310-mRNA-1
chr5_3	three_prime_UTR	53996445	53996753	53996445	ID=Msa0818310-mRNA-1:three_prime_utr;Parent=Msa0818310-mRNA-1

Gene Sequence	>Msa0818310 ATGGCTATACAGTTAAAGGATAAGACAATTTGGAGACAAGAGACAAATGGGAATGTCGGGTGTATGTTGGGGAACTGGCGTTTAGCCAGATTGAATTTCCATTTCAGGCGGCCATCAACTAGGTTTACGACAAAGCAAAACACCGTTCAAGATGAGAGCCAAGTGGAAGAAGAAGCGTATGAGGAGATTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCTAA
Protein sequence	>Msa0818310 MAIQLKDKTIWRQETNGNVGCMLGNWRLARLNFHFRRPSTRFTTKQNTVQDESQVEEEAYEEIEEEAPKDETEI*