AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1309570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309570 MtrunA17_Chr8g0336161 93.926 461 15 4 1 451 1 458 0.0 875
Msa1309570 MtrunA17_Chr7g0233351 61.752 468 147 9 1 451 1 453 0.0 520
Msa1309570 MtrunA17_Chr4g0055371 52.298 457 164 11 2 442 5 423 6.04e-127 374
Msa1309570 MtrunA17_Chr5g0424111 56.040 298 108 4 144 437 155 433 1.40e-96 297
Msa1309570 MtrunA17_Chr3g0114791 48.767 365 146 8 103 437 68 421 1.14e-92 287
Msa1309570 MtrunA17_Chr8g0377191 39.785 186 112 0 181 366 3 188 5.58e-34 126
Msa1309570 MtrunA17_Chr8g0377671 37.755 196 122 0 171 366 1 196 1.31e-33 127
Msa1309570 MtrunA17_Chr8g0377671 38.272 162 100 0 186 347 108 269 1.59e-25 105
Msa1309570 MtrunA17_Chr1g0171491 31.884 207 136 2 173 379 45 246 5.19e-28 117
Msa1309570 MtrunA17_Chr8g0382971 36.517 178 113 0 188 365 69 246 7.96e-28 113
Msa1309570 MtrunA17_Chr8g0382971 35.028 177 110 2 186 360 113 286 2.26e-21 95.1
Msa1309570 MtrunA17_Chr8g0382971 33.083 133 85 2 236 366 25 155 2.03e-13 71.2
Msa1309570 MtrunA17_Chr8g0380791 33.333 180 120 0 187 366 10 189 9.94e-27 113
Msa1309570 MtrunA17_Chr8g0380791 35.036 137 89 0 230 366 7 143 6.04e-20 92.8
Msa1309570 MtrunA17_Chr8g0380791 38.596 114 70 0 190 303 82 195 6.94e-16 80.5
Msa1309570 MtrunA17_Chr4g0059911 32.258 217 139 2 185 401 51 259 1.57e-25 107
Msa1309570 MtrunA17_Chr4g0059911 36.413 184 116 1 192 374 11 194 1.38e-24 104
Msa1309570 MtrunA17_Chr4g0059911 33.333 132 87 1 236 366 9 140 1.94e-17 83.6
Msa1309570 MtrunA17_Chr3g0131001 37.209 172 108 0 191 362 56 227 5.39e-25 108
Msa1309570 MtrunA17_Chr3g0131001 38.281 128 79 0 238 365 57 184 8.75e-16 80.1
Msa1309570 MtrunA17_Chr3g0131001 38.235 102 62 1 187 288 144 244 2.82e-11 65.9
Msa1309570 MtrunA17_Chr8g0353121 30.263 228 151 2 165 387 23 247 5.41e-25 108
Msa1309570 MtrunA17_Chr5g0424301 35.928 167 107 0 187 353 66 232 2.43e-24 106
Msa1309570 MtrunA17_Chr5g0424301 39.062 128 78 0 238 365 71 198 1.58e-21 97.8
Msa1309570 MtrunA17_Chr5g0424301 36.792 106 67 0 261 366 71 176 9.05e-16 80.1
Msa1309570 MtrunA17_Chr8g0383331 37.838 148 92 0 189 336 3 150 2.66e-24 99.0
Msa1309570 MtrunA17_Chr8g0383331 39.062 128 78 0 234 361 2 129 3.94e-23 95.5
Msa1309570 MtrunA17_Chr8g0385241 36.126 191 105 4 189 365 124 311 3.09e-24 104
Msa1309570 MtrunA17_Chr8g0385241 30.986 213 113 3 188 366 54 266 5.77e-20 91.7
Msa1309570 MtrunA17_Chr8g0385241 32.620 187 116 1 188 364 8 194 4.85e-19 89.0
Msa1309570 MtrunA17_Chr8g0385241 34.884 172 109 2 184 352 175 346 2.27e-18 87.0
Msa1309570 MtrunA17_Chr8g0385241 26.042 192 132 1 185 366 28 219 8.79e-14 73.2
Msa1309570 MtrunA17_Chr7g0256301 31.319 182 123 1 174 353 53 234 4.00e-24 105
Msa1309570 MtrunA17_Chr3g0115111 34.759 187 121 1 191 376 58 244 5.50e-24 105
Msa1309570 MtrunA17_Chr3g0115111 33.065 124 83 0 189 312 103 226 1.01e-13 73.6
Msa1309570 MtrunA17_Chr8g0385201 35.119 168 108 1 184 350 24 191 4.04e-23 102
Msa1309570 MtrunA17_Chr8g0385201 38.596 114 70 0 252 365 47 160 1.10e-17 85.9
Msa1309570 MtrunA17_Chr8g0385201 33.645 107 70 1 261 366 32 138 3.16e-13 72.0
Msa1309570 MtrunA17_Chr3g0082501 31.746 189 125 1 187 375 61 245 6.29e-23 102
Msa1309570 MtrunA17_Chr8g0377211 42.857 119 68 0 229 347 35 153 4.77e-22 92.8
Msa1309570 MtrunA17_Chr8g0377211 33.333 132 65 1 241 372 24 132 1.64e-15 74.3
Msa1309570 MtrunA17_Chr1g0180261 33.721 172 91 1 218 366 56 227 1.06e-21 98.2
Msa1309570 MtrunA17_Chr1g0180261 29.592 196 113 2 178 348 37 232 3.27e-21 97.1
Msa1309570 MtrunA17_Chr1g0180261 34.848 132 86 0 234 365 49 180 8.83e-14 73.9
Msa1309570 MtrunA17_Chr1g0180261 36.735 98 62 0 206 303 136 233 6.68e-13 70.9
Msa1309570 MtrunA17_Chr2g0316611 31.771 192 108 1 190 358 105 296 6.52e-19 88.2
Msa1309570 MtrunA17_Chr2g0316611 32.402 179 121 0 185 363 31 209 1.65e-18 87.0
Msa1309570 MtrunA17_Chr2g0316611 30.612 196 131 1 168 363 157 347 9.33e-16 78.6
Msa1309570 MtrunA17_Chr2g0316631 31.122 196 112 2 187 359 33 228 2.25e-18 85.5
Msa1309570 MtrunA17_Chr2g0316631 30.114 176 123 0 190 365 105 280 1.06e-15 77.8
Msa1309570 MtrunA17_Chr2g0316631 31.579 171 117 0 188 358 126 296 2.01e-15 76.6
Msa1309570 MtrunA17_Chr2g0316631 28.846 156 111 0 188 343 149 304 8.08e-12 65.9
Msa1309570 MtrunA17_Chr6g0467061 32.768 177 119 0 184 360 13 189 9.81e-17 81.3
Msa1309570 MtrunA17_Chr6g0467061 31.771 192 108 1 190 358 88 279 4.25e-16 79.3
Msa1309570 MtrunA17_Chr6g0467061 34.815 135 88 0 230 364 13 147 5.93e-16 79.0
Msa1309570 MtrunA17_Chr6g0467061 32.386 176 119 0 188 363 155 330 3.83e-14 73.6
Msa1309570 MtrunA17_Chr8g0383551 26.639 244 131 6 218 441 105 320 3.88e-16 79.7
Msa1309570 MtrunA17_Chr8g0377221 33.544 158 95 2 186 342 70 218 8.11e-14 71.6
Msa1309570 MtrunA17_Chr8g0377221 23.913 230 119 4 188 370 3 223 1.67e-12 67.8
Msa1309570 MtrunA17_Chr1g0210831 32.836 134 90 0 232 365 609 742 4.26e-13 71.6
Msa1309570 MtrunA17_Chr1g0210831 32.800 125 84 0 216 340 616 740 6.16e-12 67.8
Msa1309570 MtrunA17_Chr5g0398011 34.862 109 71 0 238 346 37 145 2.38e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309570 AT4G17080.1 58.047 379 133 9 86 439 128 505 8.15e-137 403
Msa1309570 AT2G35170.1 53.959 341 128 7 95 411 111 446 4.45e-117 352
Msa1309570 AT1G21920.1 55.556 315 116 5 136 437 107 410 5.44e-100 305
Msa1309570 AT1G21920.2 56.641 256 97 4 136 378 107 361 3.04e-94 289
Msa1309570 AT1G77660.1 50.909 330 135 3 128 441 100 418 2.61e-93 288
Msa1309570 AT3G56960.2 32.857 210 135 2 168 375 48 253 6.29e-30 123
Msa1309570 AT3G56960.1 32.857 210 135 2 168 375 48 253 6.29e-30 123
Msa1309570 AT2G41210.1 35.450 189 118 1 187 375 67 251 7.10e-30 123
Msa1309570 AT1G60890.1 37.778 180 112 0 187 366 8 187 1.57e-29 122
Msa1309570 AT1G60890.1 38.843 121 66 2 189 309 102 214 9.21e-14 73.9
Msa1309570 AT1G60890.1 37.168 113 71 0 191 303 81 193 2.92e-13 72.4
Msa1309570 AT1G60890.2 37.778 180 112 0 187 366 20 199 1.62e-29 122
Msa1309570 AT1G60890.2 38.843 121 66 2 189 309 114 226 9.88e-14 73.9
Msa1309570 AT1G60890.2 37.168 113 71 0 191 303 93 205 3.11e-13 72.4
Msa1309570 AT1G21980.1 33.014 209 130 2 152 360 48 246 1.84e-27 116
Msa1309570 AT1G21980.1 39.062 128 78 0 238 365 78 205 7.84e-22 99.0
Msa1309570 AT1G21980.1 36.792 106 67 0 261 366 78 183 1.49e-15 79.7
Msa1309570 AT3G07960.1 32.804 189 123 1 187 375 24 208 1.05e-26 114
Msa1309570 AT3G07960.3 32.804 189 123 1 187 375 24 208 1.20e-26 113
Msa1309570 AT3G07960.2 32.804 189 123 1 187 375 24 208 1.37e-26 113
Msa1309570 AT3G07960.4 32.804 189 123 1 187 375 24 208 1.37e-26 113
Msa1309570 AT1G10900.2 37.222 180 113 0 187 366 8 187 1.38e-26 113
Msa1309570 AT1G10900.2 31.111 135 93 0 231 365 6 140 8.47e-15 77.4
Msa1309570 AT1G10900.2 39.091 110 67 0 194 303 84 193 3.94e-13 72.0
Msa1309570 AT1G10900.2 40.000 90 54 0 189 278 102 191 1.41e-11 67.4
Msa1309570 AT1G10900.1 37.222 180 113 0 187 366 8 187 1.38e-26 113
Msa1309570 AT1G10900.1 31.111 135 93 0 231 365 6 140 8.47e-15 77.4
Msa1309570 AT1G10900.1 39.091 110 67 0 194 303 84 193 3.94e-13 72.0
Msa1309570 AT1G10900.1 40.000 90 54 0 189 278 102 191 1.41e-11 67.4
Msa1309570 AT1G10900.3 37.222 180 113 0 187 366 8 187 1.38e-26 113
Msa1309570 AT1G10900.3 31.111 135 93 0 231 365 6 140 8.47e-15 77.4
Msa1309570 AT1G10900.3 39.091 110 67 0 194 303 84 193 3.94e-13 72.0
Msa1309570 AT1G10900.3 40.000 90 54 0 189 278 102 191 1.41e-11 67.4
Msa1309570 AT2G26420.2 34.595 185 119 1 178 360 39 223 3.00e-26 112
Msa1309570 AT2G26420.2 35.938 128 82 0 238 365 55 182 1.03e-17 86.3
Msa1309570 AT2G26420.2 33.962 106 70 0 261 366 55 160 2.93e-12 69.3
Msa1309570 AT2G26420.3 34.595 185 119 1 178 360 39 223 3.00e-26 112
Msa1309570 AT2G26420.3 35.938 128 82 0 238 365 55 182 1.03e-17 86.3
Msa1309570 AT2G26420.3 33.962 106 70 0 261 366 55 160 2.93e-12 69.3
Msa1309570 AT2G26420.1 34.595 185 119 1 178 360 39 223 3.70e-26 112
Msa1309570 AT2G26420.1 35.938 128 82 0 238 365 55 182 1.29e-17 85.9
Msa1309570 AT2G26420.1 33.962 106 70 0 261 366 55 160 3.27e-12 69.3
Msa1309570 AT1G77740.2 34.706 170 111 0 191 360 78 247 1.97e-24 107
Msa1309570 AT1G77740.2 39.844 128 77 0 238 365 79 206 5.38e-22 99.8
Msa1309570 AT1G77740.1 34.706 170 111 0 191 360 78 247 1.97e-24 107
Msa1309570 AT1G77740.1 39.844 128 77 0 238 365 79 206 5.38e-22 99.8
Msa1309570 AT3G09920.3 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1309570 AT3G09920.3 35.185 108 70 0 261 368 55 162 1.33e-14 76.6
Msa1309570 AT3G09920.3 37.069 116 73 0 188 303 120 235 5.17e-14 75.1
Msa1309570 AT3G09920.4 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1309570 AT3G09920.4 35.185 108 70 0 261 368 55 162 1.33e-14 76.6
Msa1309570 AT3G09920.4 37.069 116 73 0 188 303 120 235 5.17e-14 75.1
Msa1309570 AT3G09920.2 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1309570 AT3G09920.2 35.185 108 70 0 261 368 55 162 1.33e-14 76.6
Msa1309570 AT3G09920.2 37.069 116 73 0 188 303 120 235 5.17e-14 75.1
Msa1309570 AT3G09920.1 30.000 210 145 1 157 366 22 229 3.02e-23 103
Msa1309570 AT3G09920.1 35.185 108 70 0 261 368 55 162 1.33e-14 76.6
Msa1309570 AT3G09920.1 37.069 116 73 0 188 303 120 235 5.17e-14 75.1
Msa1309570 AT1G75010.1 32.000 125 85 0 216 340 605 729 4.20e-11 65.9
Msa1309570 AT1G75010.1 34.286 105 69 0 259 363 602 706 4.47e-11 65.5

Find 142 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCAATATTGTGGAAATT+AGG 0.064253 8_4:-6051392 Msa1309570:CDS
ATTGAAATTGAAATTGAAAT+TGG 0.184005 8_4:+6051734 None:intergenic
GGGGAATAGGGTTAGGGATT+TGG 0.223176 8_4:+6051755 None:intergenic
AACCCTAATTTCCACAATAT+TGG 0.227162 8_4:+6051389 None:intergenic
GAGTAAGGGTAGTAGGTATA+AGG 0.242613 8_4:-6051220 Msa1309570:CDS
ATCCAATATTGTGGAAATTA+GGG 0.251880 8_4:-6051391 Msa1309570:CDS
TGAAATTGAAATTGGGGAAT+AGG 0.258224 8_4:+6051742 None:intergenic
TTGAAATTGAAATTGAAATT+GGG 0.281485 8_4:+6051735 None:intergenic
GAAATGGGTTTGGTGTTTAT+AGG 0.300633 8_4:-6051176 Msa1309570:CDS
TATGAGGATGAAAGAGATTA+GGG 0.301966 8_4:+6051859 None:intergenic
GAAATTGAAATTGGGGAATA+GGG 0.305926 8_4:+6051743 None:intergenic
TGGTGTTGAGAGTTGGAGTA+AGG 0.329589 8_4:-6051235 Msa1309570:CDS
TATGAAGGGGATTGGGTTGA+TGG 0.332229 8_4:-6051273 Msa1309570:CDS
CGGACAAGATGCATGGATTC+GGG 0.332377 8_4:-6049678 Msa1309570:CDS
GATGGGAAATATGAAGGTTT+TGG 0.332995 8_4:-6051255 Msa1309570:CDS
CTTGTTAATAAGATGTTCAT+TGG 0.356604 8_4:-6048127 Msa1309570:three_prime_UTR
TATAGAGATGGATTGAGAAA+TGG 0.358776 8_4:-6051192 Msa1309570:CDS
GTCTTGGCCACTATCACTTT+AGG 0.368209 8_4:-6051032 Msa1309570:intron
TTATGAGGATGAAAGAGATT+AGG 0.372460 8_4:+6051858 None:intergenic
TTGACACCAACAGGGGAAAC+TGG 0.381788 8_4:+6049499 None:intergenic
AGAAGAAAGGGAGGGAAAGA+AGG 0.382410 8_4:+6051607 None:intergenic
TTACAACAATAGCTGGGTTA+AGG 0.384562 8_4:+6048243 None:intergenic
TGAAATTGGGGAATAGGGTT+AGG 0.385743 8_4:+6051748 None:intergenic
CTGGAGTGATTGACACCAAC+AGG 0.386537 8_4:+6049490 None:intergenic
ATCTACCTTGGCTACATCAT+AGG 0.396492 8_4:+6048674 None:intergenic
GATTTGTTGGTGAGTTTAAG+TGG 0.397616 8_4:-6051068 Msa1309570:CDS
AGCAGCTACTCTGGCTGCAT+TGG 0.398227 8_4:+6048611 None:intergenic
GCGGACAAGATGCATGGATT+CGG 0.398614 8_4:-6049679 Msa1309570:CDS
ATATTGTGGAAATTAGGGTT+AGG 0.403227 8_4:-6051386 Msa1309570:CDS
AGAGTTGGAGTAAGGGTAGT+AGG 0.407656 8_4:-6051227 Msa1309570:CDS
ATAGAGATGGATTGAGAAAT+GGG 0.407814 8_4:-6051191 Msa1309570:CDS
AGGGTTAGGGTTTATGCAAA+TGG 0.414027 8_4:-6051372 Msa1309570:CDS
AGTAAGGGTAGTAGGTATAA+GGG 0.424632 8_4:-6051219 Msa1309570:CDS
TGGGTTGATGGGAAATATGA+AGG 0.426845 8_4:-6051261 Msa1309570:CDS
GATGATGGGAGTAGATTTGT+TGG 0.429175 8_4:-6051081 Msa1309570:CDS
TGGGATCTTACAACAATAGC+TGG 0.430401 8_4:+6048236 None:intergenic
AAAGAGATTAGGGTTGAAAT+CGG 0.437710 8_4:+6051869 None:intergenic
ATTTGTTGGTGAGTTTAAGT+GGG 0.438217 8_4:-6051067 Msa1309570:CDS
CGGTGGTGATTTGAATTTGA+GGG 0.439339 8_4:+6051497 None:intergenic
GCAAATGGCCATCGTTATGA+AGG 0.442940 8_4:-6049643 Msa1309570:CDS
GAATAGGGTTAGGGATTTGG+AGG 0.443890 8_4:+6051758 None:intergenic
TATTGTGGAAATTAGGGTTA+GGG 0.446996 8_4:-6051385 Msa1309570:CDS
GATTTATTATCTATCTGCTA+TGG 0.447077 8_4:-6048398 Msa1309570:three_prime_UTR
GTGGAAGATATGAAGGGGAT+TGG 0.450612 8_4:-6051281 Msa1309570:CDS
GGAATGTATACATTTAGAAA+TGG 0.453121 8_4:-6049589 Msa1309570:CDS
AGTTGATAGCCTCACAAAAC+AGG 0.458611 8_4:+6048531 None:intergenic
TGAAATTGAAATTGAAATTG+GGG 0.459705 8_4:+6051736 None:intergenic
GAAATTGGGGAATAGGGTTA+GGG 0.462785 8_4:+6051749 None:intergenic
AATATGAGTGGAAGATATGA+AGG 0.464536 8_4:-6051288 Msa1309570:CDS
TGGAAGATATGAAGGGGATT+GGG 0.467735 8_4:-6051280 Msa1309570:CDS
AGCCTTGACAGCAGCTACTC+TGG 0.475041 8_4:+6048602 None:intergenic
GGTGTTGAGAGTTGGAGTAA+GGG 0.478917 8_4:-6051234 Msa1309570:CDS
GAGAAACTCAATCTGGTCAC+TGG 0.482465 8_4:-6049567 Msa1309570:CDS
TATACTGGGGATGTTTATGC+TGG 0.487144 8_4:-6051150 Msa1309570:CDS
ATTTATTATCTATCTGCTAT+GGG 0.487475 8_4:-6048397 Msa1309570:three_prime_UTR
GAGGGGAAAAGACATGGTCT+TGG 0.489572 8_4:-6049610 Msa1309570:CDS
AAACTCACCTAAAGTGATAG+TGG 0.490431 8_4:+6051025 None:intergenic
AGAAGAAAGAGTGAGTAGGT+GGG 0.490534 8_4:+6051571 None:intergenic
GTCAGCAACTGTGGAGATGA+TGG 0.491405 8_4:+6048433 None:intergenic
TGGGGTGTTAAACATGGTCT+TGG 0.492897 8_4:-6051048 Msa1309570:CDS
ACATATCCAGTTTCCCCTGT+TGG 0.495511 8_4:-6049505 Msa1309570:CDS
GGTGTTCATACTTGTGATGA+TGG 0.496834 8_4:-6051096 Msa1309570:CDS
CGGAGAAAGCGAAAGCAGAT+AGG 0.497218 8_4:+6051541 None:intergenic
AAGAAGAAAGAGTGAGTAGG+TGG 0.498494 8_4:+6051570 None:intergenic
AATGAGAAGTGTTGTGGGAG+TGG 0.498578 8_4:-6051324 Msa1309570:CDS
TATGTGGCGTTCTGCGTGCT+TGG 0.500334 8_4:+6049523 None:intergenic
ATGAAGGGGATTGGGTTGAT+GGG 0.500799 8_4:-6051272 Msa1309570:CDS
ATGAGAAGTGTTGTGGGAGT+GGG 0.501259 8_4:-6051323 Msa1309570:CDS
TATGCTGGTGAATGGTTGAA+TGG 0.503530 8_4:-6051135 Msa1309570:CDS
ATTGAATTGAAAAGTTTATG+AGG 0.504198 8_4:+6051843 None:intergenic
CTTTAACTGACTTGGAAATG+AGG 0.507005 8_4:-6048164 Msa1309570:three_prime_UTR
GATATTTGCTTTAACTGACT+TGG 0.507054 8_4:-6048172 Msa1309570:three_prime_UTR
TGTTTCACTGTGAGGACTAT+CGG 0.507879 8_4:+6051465 None:intergenic
GGTTGAATGGTCAGAGTCAT+GGG 0.508114 8_4:-6051122 Msa1309570:CDS
GGGAAAGAAGGAGGATTAGA+CGG 0.508839 8_4:+6051619 None:intergenic
GAAGAAAGAGTGAGTAGGTG+GGG 0.509004 8_4:+6051572 None:intergenic
AGAAATGGAGAAACTCAATC+TGG 0.509744 8_4:-6049574 Msa1309570:CDS
ATTTACTCGTTCATCTACCT+TGG 0.514543 8_4:+6048662 None:intergenic
GTGGCGGAGACGGCGGAGTG+CGG 0.516080 8_4:+6051647 None:intergenic
AGATTTGTAGCTAGCTACTC+TGG 0.516897 8_4:-6048468 Msa1309570:three_prime_UTR
GATGGATTGAGAAATGGGTT+TGG 0.521028 8_4:-6051186 Msa1309570:CDS
GGGATGTTTATGCTGGTGAA+TGG 0.523748 8_4:-6051143 Msa1309570:CDS
GCGGAGACGGCGGAGTGCGG+CGG 0.525703 8_4:+6051650 None:intergenic
GACGGAAACGGTGGCGGAGA+CGG 0.530033 8_4:+6051637 None:intergenic
TGAAGAATGAGAAGTGTTGT+GGG 0.537863 8_4:-6051329 Msa1309570:CDS
GCGGAGTGCGGCGGATGAGA+TGG 0.539666 8_4:+6051659 None:intergenic
GGATTAGACGGAAACGGTGG+CGG 0.540352 8_4:+6051631 None:intergenic
TATAAGGGTATGTATAGAGA+TGG 0.544385 8_4:-6051204 Msa1309570:CDS
GTGAAGAAATAGAATTGTGT+TGG 0.554593 8_4:+6051808 None:intergenic
AGAAAGGGAGGGAAAGAAGG+AGG 0.556007 8_4:+6051610 None:intergenic
TCATGTAGAGTCGTATATGC+TGG 0.556815 8_4:-6048279 Msa1309570:three_prime_UTR
TTTGTTGGTGAGTTTAAGTG+GGG 0.563464 8_4:-6051066 Msa1309570:CDS
AGTCGTATATGCTGGTGTGC+TGG 0.566878 8_4:-6048271 Msa1309570:three_prime_UTR
TGGTTGAATGGTCAGAGTCA+TGG 0.568619 8_4:-6051123 Msa1309570:CDS
ACGGTGGTGATTTGAATTTG+AGG 0.571474 8_4:+6051496 None:intergenic
CTGCACTGTATTAAGTACTC+TGG 0.572225 8_4:+6049471 None:intergenic
AGAAATGGAGATACGTATGC+TGG 0.572290 8_4:-6049712 Msa1309570:CDS
AGCCAGAGTAGCTGCTGTCA+AGG 0.572431 8_4:-6048604 Msa1309570:CDS
GAAGGAGGATTAGACGGAAA+CGG 0.574183 8_4:+6051625 None:intergenic
AACAGGGGAAACTGGATATG+TGG 0.576987 8_4:+6049507 None:intergenic
GTTTATTACTATAATATGAG+TGG 0.579922 8_4:-6051300 Msa1309570:CDS
TGAGAAGTGTTGTGGGAGTG+GGG 0.582204 8_4:-6051322 Msa1309570:CDS
GCCATCGTTATGAAGGAACC+TGG 0.582273 8_4:-6049636 Msa1309570:CDS
ATGGGTCTTGTATAGAGAGA+GGG 0.583170 8_4:-6048379 Msa1309570:three_prime_UTR
TTTAAGTGGGGTGTTAAACA+TGG 0.583836 8_4:-6051054 Msa1309570:CDS
ATATGAGTGGAAGATATGAA+GGG 0.589019 8_4:-6051287 Msa1309570:CDS
TTAAAGAAGAAAGAGTGAGT+AGG 0.590259 8_4:+6051567 None:intergenic
TATGAAGGAACCTGGCATGA+GGG 0.590379 8_4:-6049628 Msa1309570:CDS
CAGAGTACTTAATACAGTGC+AGG 0.593895 8_4:-6049470 Msa1309570:intron
ATGAAGGAACCTGGCATGAG+GGG 0.602811 8_4:-6049627 Msa1309570:CDS
ATGAAGAATGAGAAGTGTTG+TGG 0.603885 8_4:-6051330 Msa1309570:CDS
TGGCATGAGGGGAAAAGACA+TGG 0.607412 8_4:-6049616 Msa1309570:CDS
GCAAATGGTGATGTGTATGA+AGG 0.608050 8_4:-6051357 Msa1309570:CDS
GGAAACGGTGGCGGAGACGG+CGG 0.612548 8_4:+6051640 None:intergenic
TTATGAAGGAACCTGGCATG+AGG 0.612995 8_4:-6049629 Msa1309570:CDS
GGGATCTTACAACAATAGCT+GGG 0.613050 8_4:+6048237 None:intergenic
TATATTGCGGACAAGATGCA+TGG 0.616225 8_4:-6049685 Msa1309570:CDS
GAGTGCGGCGGATGAGATGG+CGG 0.619453 8_4:+6051662 None:intergenic
CAGTGAAACAGTGGAATCGA+CGG 0.623242 8_4:-6051452 Msa1309570:CDS
GTGTTCATACTTGTGATGAT+GGG 0.626133 8_4:-6051095 Msa1309570:CDS
GCCAGGTTCCTTCATAACGA+TGG 0.629240 8_4:+6049635 None:intergenic
TATGGGTCTTGTATAGAGAG+AGG 0.629329 8_4:-6048380 Msa1309570:three_prime_UTR
AGGACTATCGGAAGAGACGA+CGG 0.633764 8_4:+6051477 None:intergenic
TGGAGTGATTGACACCAACA+GGG 0.636221 8_4:+6049491 None:intergenic
GAAAGCCTATGATGTAGCCA+AGG 0.637335 8_4:-6048679 Msa1309570:CDS
AATAGGGTTAGGGATTTGGA+GGG 0.639807 8_4:+6051759 None:intergenic
TTTGTAGCTAGCTACTCTGG+TGG 0.640557 8_4:-6048465 Msa1309570:three_prime_UTR
TTGCGTTCAGTCAGCAACTG+TGG 0.650991 8_4:+6048424 None:intergenic
TATGAGTGGAAGATATGAAG+GGG 0.653354 8_4:-6051286 Msa1309570:CDS
AATGGTCAGAGTCATGGGAG+TGG 0.666277 8_4:-6051117 Msa1309570:CDS
TAGTCCTCACAGTGAAACAG+TGG 0.670821 8_4:-6051461 Msa1309570:CDS
GATTCCACTGTTTCACTGTG+AGG 0.673057 8_4:+6051457 None:intergenic
TCAAAACATCTTGCGCACGA+TGG 0.680906 8_4:+6048193 None:intergenic
GTATGCTGGTGAGTATATTG+CGG 0.683410 8_4:-6049698 Msa1309570:CDS
AAGACAGAAGAAAAGTGCGG+CGG 0.688106 8_4:+6051521 None:intergenic
GGAGGATTAGACGGAAACGG+TGG 0.701072 8_4:+6051628 None:intergenic
GGACAAGATGCATGGATTCG+GGG 0.701613 8_4:-6049677 Msa1309570:CDS
GTCTTGTATAGAGAGAGGGA+AGG 0.707455 8_4:-6048375 Msa1309570:three_prime_UTR
GGAAAGACAGAAGAAAAGTG+CGG 0.713433 8_4:+6051518 None:intergenic
TGAAACAGTGGAATCGACGG+CGG 0.724376 8_4:-6051449 Msa1309570:CDS
GGAGTGATTGACACCAACAG+GGG 0.733255 8_4:+6049492 None:intergenic
ACTATCGGAAGAGACGACGG+TGG 0.753609 8_4:+6051480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGATTTTATGATGAAT+TGG + chr8_4:6048352-6048371 None:intergenic 15.0%
!! TTGAAATTGAAATTGAAATT+GGG + chr8_4:6048403-6048422 None:intergenic 15.0%
!! ATTGAAATTGAAATTGAAAT+TGG + chr8_4:6048404-6048423 None:intergenic 15.0%
!! TATATTTGAGAAAAATAAGA+AGG + chr8_4:6050266-6050285 None:intergenic 15.0%
!!! TTCTTATTTTTCTCAAATAT+AGG - chr8_4:6050265-6050284 Msa1309570:intron 15.0%
!! AAATAGTTATACTTATACTA+TGG + chr8_4:6050374-6050393 None:intergenic 15.0%
!!! AGTATAAGTATAACTATTTT+TGG - chr8_4:6050375-6050394 Msa1309570:intron 15.0%
!!! GTATAAGTATAACTATTTTT+GGG - chr8_4:6050376-6050395 Msa1309570:intron 15.0%
!!! TATAAGTATAACTATTTTTG+GGG - chr8_4:6050377-6050396 Msa1309570:intron 15.0%
!!! TATTTTCATTTCTTTAGAAA+TGG - chr8_4:6050408-6050427 Msa1309570:intron 15.0%
!! AAAAAATCAGAAATTCAGAT+CGG - chr8_4:6048220-6048239 Msa1309570:three_prime_UTR 20.0%
!! ATTGAATTGAAAAGTTTATG+AGG + chr8_4:6048295-6048314 None:intergenic 20.0%
!! TGAAATTGAAATTGAAATTG+GGG + chr8_4:6048402-6048421 None:intergenic 20.0%
!!! ATTTTTTAGTGGAAAATTGT+GGG + chr8_4:6048429-6048448 None:intergenic 20.0%
!!! ATGAAAAGATGATTTTTTAG+TGG + chr8_4:6048440-6048459 None:intergenic 20.0%
!! ATAGTAAAAAGAAGAAGAAA+GGG + chr8_4:6048543-6048562 None:intergenic 20.0%
!! GTTTATTACTATAATATGAG+TGG - chr8_4:6048835-6048854 Msa1309570:intron 20.0%
!! AATATATTCCACCTAATATT+TGG + chr8_4:6049492-6049511 None:intergenic 20.0%
!! AATATTAGGTGGAATATATT+TGG - chr8_4:6049492-6049511 Msa1309570:CDS 20.0%
!! TAATATTGCATCTGTTTATA+GGG - chr8_4:6049782-6049801 Msa1309570:intron 20.0%
!! TTCACGTTAAGTATATTAAA+GGG + chr8_4:6050064-6050083 None:intergenic 20.0%
!! TTCAACAAAAAAACAGAATA+GGG + chr8_4:6050153-6050172 None:intergenic 20.0%
!! TTTCAACAAAAAAACAGAAT+AGG + chr8_4:6050154-6050173 None:intergenic 20.0%
!!! ATTGTTGAATTGATTTATGT+AGG - chr8_4:6050322-6050341 Msa1309570:intron 20.0%
!!! TTGTTGAATTGATTTATGTA+GGG - chr8_4:6050323-6050342 Msa1309570:intron 20.0%
!!! ATAAGTATAACTATTTTTGG+GGG - chr8_4:6050378-6050397 Msa1309570:intron 20.0%
!! ACTAAAAGAAAAGAAAATAG+AGG - chr8_4:6050751-6050770 Msa1309570:intron 20.0%
!! CATATCATTCTAATTATTCT+AGG + chr8_4:6050815-6050834 None:intergenic 20.0%
!! AAAATTACACTAAAACACAA+CGG + chr8_4:6050960-6050979 None:intergenic 20.0%
!! ATTTATTATCTATCTGCTAT+GGG - chr8_4:6051738-6051757 Msa1309570:CDS 20.0%
!!! TCTCTTCTTATTTTTACTAT+GGG + chr8_4:6051921-6051940 None:intergenic 20.0%
!!! TTCTCTTCTTATTTTTACTA+TGG + chr8_4:6051922-6051941 None:intergenic 20.0%
!!! GATTTTTTAGTGGAAAATTG+TGG + chr8_4:6048430-6048449 None:intergenic 25.0%
! GATAGTAAAAAGAAGAAGAA+AGG + chr8_4:6048544-6048563 None:intergenic 25.0%
!!! GGATGAATTTTTCGAATTTT+TGG + chr8_4:6048728-6048747 None:intergenic 25.0%
! GAAAAATTCATCCAATATTG+TGG - chr8_4:6048735-6048754 Msa1309570:intron 25.0%
! ATCCAATATTGTGGAAATTA+GGG - chr8_4:6048744-6048763 Msa1309570:intron 25.0%
!!! GTGTTTATAGGTTTTATACT+GGG - chr8_4:6048971-6048990 Msa1309570:intron 25.0%
!!! TGTTTATAGGTTTTATACTG+GGG - chr8_4:6048972-6048991 Msa1309570:intron 25.0%
! TAAATTACTAATGGTGTATC+TGG - chr8_4:6049278-6049297 Msa1309570:intron 25.0%
! AAATTACTAATGGTGTATCT+GGG - chr8_4:6049279-6049298 Msa1309570:intron 25.0%
! ATATTTGGATTTCAGATGTT+AGG - chr8_4:6049507-6049526 Msa1309570:CDS 25.0%
!! ATTTCAGATGTTAGGTTTTA+AGG - chr8_4:6049515-6049534 Msa1309570:CDS 25.0%
!!! ATCTGTTTTTGATCATTCTT+TGG - chr8_4:6049613-6049632 Msa1309570:CDS 25.0%
! TTAAGAATATAGTAGACAGA+TGG - chr8_4:6049720-6049739 Msa1309570:intron 25.0%
! GTAATATTGCATCTGTTTAT+AGG - chr8_4:6049781-6049800 Msa1309570:intron 25.0%
! AATATTGCATCTGTTTATAG+GGG - chr8_4:6049783-6049802 Msa1309570:intron 25.0%
! AAAGTAACGAAAACAAAAAG+TGG + chr8_4:6049809-6049828 None:intergenic 25.0%
! CAGCAAGTAAAATTAAAATG+CGG + chr8_4:6050002-6050021 None:intergenic 25.0%
! TCACGTTAAGTATATTAAAG+GGG + chr8_4:6050063-6050082 None:intergenic 25.0%
! GTTCACGTTAAGTATATTAA+AGG + chr8_4:6050065-6050084 None:intergenic 25.0%
!!! GACATGTTTTTAATGAATAG+TGG - chr8_4:6050213-6050232 Msa1309570:intron 25.0%
! TATACTATGGAGCAAATAAA+AGG + chr8_4:6050361-6050380 None:intergenic 25.0%
! GGAATGTATACATTTAGAAA+TGG - chr8_4:6050546-6050565 Msa1309570:intron 25.0%
! GAGCATTTAACAATAGATTA+AGG + chr8_4:6050717-6050736 None:intergenic 25.0%
!! TTTTCTTTTAGTGTTAGCTA+GGG + chr8_4:6050743-6050762 None:intergenic 25.0%
!! AGTAAGTCTTATAGTTTGAT+AGG - chr8_4:6050871-6050890 Msa1309570:intron 25.0%
!! GTAAGTCTTATAGTTTGATA+GGG - chr8_4:6050872-6050891 Msa1309570:intron 25.0%
! TAAAAAAATTCATGTACTGC+TGG - chr8_4:6050995-6051014 Msa1309570:intron 25.0%
! GCTATGTATTAAGGAAATAT+TGG - chr8_4:6051094-6051113 Msa1309570:CDS 25.0%
!! ATTGGACATTTTCTTCTATA+TGG - chr8_4:6051292-6051311 Msa1309570:CDS 25.0%
!!! ATCAACTACAACTTTTGTTT+TGG - chr8_4:6051620-6051639 Msa1309570:CDS 25.0%
! GATTTATTATCTATCTGCTA+TGG - chr8_4:6051737-6051756 Msa1309570:CDS 25.0%
! ACATGAATTTACAAAAATGC+AGG + chr8_4:6051842-6051861 None:intergenic 25.0%
! CTTGTTAATAAGATGTTCAT+TGG - chr8_4:6052008-6052027 Msa1309570:five_prime_UTR 25.0%
! CTTCACTTTCTCTTTTTGTT+TGG + chr8_4:6048139-6048158 None:intergenic 30.0%
AAAGAGATTAGGGTTGAAAT+CGG + chr8_4:6048269-6048288 None:intergenic 30.0%
TATGAGGATGAAAGAGATTA+GGG + chr8_4:6048279-6048298 None:intergenic 30.0%
TTATGAGGATGAAAGAGATT+AGG + chr8_4:6048280-6048299 None:intergenic 30.0%
!!! TAGAATTGTGTTGGTTTTTG+TGG + chr8_4:6048321-6048340 None:intergenic 30.0%
GTGAAGAAATAGAATTGTGT+TGG + chr8_4:6048330-6048349 None:intergenic 30.0%
GAAATTGAAATTGGGGAATA+GGG + chr8_4:6048395-6048414 None:intergenic 30.0%
TGAAATTGAAATTGGGGAAT+AGG + chr8_4:6048396-6048415 None:intergenic 30.0%
TAAAAAGAAGAAGAAAGGGA+GGG + chr8_4:6048539-6048558 None:intergenic 30.0%
TTAAAGAAGAAAGAGTGAGT+AGG + chr8_4:6048571-6048590 None:intergenic 30.0%
CATCCAATATTGTGGAAATT+AGG - chr8_4:6048743-6048762 Msa1309570:intron 30.0%
AACCCTAATTTCCACAATAT+TGG + chr8_4:6048749-6048768 None:intergenic 30.0%
ATATTGTGGAAATTAGGGTT+AGG - chr8_4:6048749-6048768 Msa1309570:intron 30.0%
TATTGTGGAAATTAGGGTTA+GGG - chr8_4:6048750-6048769 Msa1309570:intron 30.0%
AATATGAGTGGAAGATATGA+AGG - chr8_4:6048847-6048866 Msa1309570:intron 30.0%
ATATGAGTGGAAGATATGAA+GGG - chr8_4:6048848-6048867 Msa1309570:intron 30.0%
! TATAAGGGTATGTATAGAGA+TGG - chr8_4:6048931-6048950 Msa1309570:intron 30.0%
!! TATAGAGATGGATTGAGAAA+TGG - chr8_4:6048943-6048962 Msa1309570:intron 30.0%
!! ATAGAGATGGATTGAGAAAT+GGG - chr8_4:6048944-6048963 Msa1309570:intron 30.0%
!! GGTGTTTATAGGTTTTATAC+TGG - chr8_4:6048970-6048989 Msa1309570:intron 30.0%
! ATTTGTTGGTGAGTTTAAGT+GGG - chr8_4:6049068-6049087 Msa1309570:intron 30.0%
! GTTTTGTGTGAATCAATGAT+TGG - chr8_4:6049412-6049431 Msa1309570:intron 30.0%
GTGAATCAATGATTGGAATT+TGG - chr8_4:6049419-6049438 Msa1309570:intron 30.0%
ACATGTCATAATAAGTTAGC+TGG + chr8_4:6049592-6049611 None:intergenic 30.0%
AATGCTGAGTTTAGATTCAA+AGG - chr8_4:6049846-6049865 Msa1309570:intron 30.0%
! ACTTGCTGCTAATTTAAGTT+TGG - chr8_4:6050014-6050033 Msa1309570:intron 30.0%
CACGTTAAGTATATTAAAGG+GGG + chr8_4:6050062-6050081 None:intergenic 30.0%
TCAGGATATAAAACAGGATA+AGG + chr8_4:6050241-6050260 None:intergenic 30.0%
AAGGATTCAGGATATAAAAC+AGG + chr8_4:6050247-6050266 None:intergenic 30.0%
GAGAAAAATAAGAAGGATTC+AGG + chr8_4:6050259-6050278 None:intergenic 30.0%
ATTCAACAATAACGCACTAA+CGG + chr8_4:6050312-6050331 None:intergenic 30.0%
! CTTTTCTTTTAGTGTTAGCT+AGG + chr8_4:6050744-6050763 None:intergenic 30.0%
TCAAACTATAAGACTTACTC+AGG + chr8_4:6050870-6050889 None:intergenic 30.0%
!! GACTTGTTGAAAAATCTGAT+TGG - chr8_4:6050931-6050950 Msa1309570:intron 30.0%
TATATGAAACTTGAGACAGA+TGG + chr8_4:6051024-6051043 None:intergenic 30.0%
CAGAACAATGCTATGTATTA+AGG - chr8_4:6051085-6051104 Msa1309570:CDS 30.0%
GAAATTTCGACAAAATGAGA+TGG + chr8_4:6051213-6051232 None:intergenic 30.0%
! TCTCATTTTGTCGAAATTTC+TGG - chr8_4:6051213-6051232 Msa1309570:CDS 30.0%
TTTGTAAAAGCTCGAGATTA+TGG - chr8_4:6051262-6051281 Msa1309570:CDS 30.0%
AGCACATTTGTGCAAAATAA+AGG - chr8_4:6051323-6051342 Msa1309570:CDS 30.0%
!!! TATCTGTTTGCAGTGTTTTT+TGG + chr8_4:6051359-6051378 None:intergenic 30.0%
CACGAACTTTCATTATTAAC+CGG - chr8_4:6051394-6051413 Msa1309570:CDS 30.0%
ACGAACTTTCATTATTAACC+GGG - chr8_4:6051395-6051414 Msa1309570:CDS 30.0%
GATATTTGCTTTAACTGACT+TGG - chr8_4:6051963-6051982 Msa1309570:five_prime_UTR 30.0%
GTAAAAAGAAGAAGAAAGGG+AGG + chr8_4:6048540-6048559 None:intergenic 35.0%
!! ATGAAGAATGAGAAGTGTTG+TGG - chr8_4:6048805-6048824 Msa1309570:intron 35.0%
!! TGAAGAATGAGAAGTGTTGT+GGG - chr8_4:6048806-6048825 Msa1309570:intron 35.0%
TATGAGTGGAAGATATGAAG+GGG - chr8_4:6048849-6048868 Msa1309570:intron 35.0%
! GATGGGAAATATGAAGGTTT+TGG - chr8_4:6048880-6048899 Msa1309570:intron 35.0%
AGTAAGGGTAGTAGGTATAA+GGG - chr8_4:6048916-6048935 Msa1309570:intron 35.0%
!! GAAATGGGTTTGGTGTTTAT+AGG - chr8_4:6048959-6048978 Msa1309570:intron 35.0%
GTGTTCATACTTGTGATGAT+GGG - chr8_4:6049040-6049059 Msa1309570:intron 35.0%
!! GATTTGTTGGTGAGTTTAAG+TGG - chr8_4:6049067-6049086 Msa1309570:intron 35.0%
! TTTGTTGGTGAGTTTAAGTG+GGG - chr8_4:6049069-6049088 Msa1309570:intron 35.0%
! TTTAAGTGGGGTGTTAAACA+TGG - chr8_4:6049081-6049100 Msa1309570:intron 35.0%
AAACTCACCTAAAGTGATAG+TGG + chr8_4:6049113-6049132 None:intergenic 35.0%
CAATCACAATTCAAGAACAC+CGG + chr8_4:6049317-6049336 None:intergenic 35.0%
!!! GAGTTTTATTCCTGTGTTTG+TGG - chr8_4:6049441-6049460 Msa1309570:intron 35.0%
TGTTTGTGGATATCAGTAGT+TGG - chr8_4:6049455-6049474 Msa1309570:intron 35.0%
! TATCAGTAGTTGGTTCAGTT+TGG - chr8_4:6049465-6049484 Msa1309570:intron 35.0%
! ATCAGTAGTTGGTTCAGTTT+GGG - chr8_4:6049466-6049485 Msa1309570:intron 35.0%
TTCAGTTTGGGCCAAATATT+AGG - chr8_4:6049478-6049497 Msa1309570:CDS 35.0%
!!! GTTTTAAGGATTAGTGACGT+AGG - chr8_4:6049529-6049548 Msa1309570:CDS 35.0%
!!! TGGTTGGTTTTGACATGTTT+AGG - chr8_4:6049679-6049698 Msa1309570:CDS 35.0%
!!! GGTTGGTTTTGACATGTTTA+GGG - chr8_4:6049680-6049699 Msa1309570:CDS 35.0%
! TCGTTACTTTATGTAACCTG+TGG - chr8_4:6049819-6049838 Msa1309570:intron 35.0%
TGTTGAAAAACTGTGCTTGT+TGG - chr8_4:6050166-6050185 Msa1309570:intron 35.0%
ATATAGGTGCATCTCTTCTA+AGG - chr8_4:6050281-6050300 Msa1309570:intron 35.0%
ACTATGGAGCAAATAAAAGG+AGG + chr8_4:6050358-6050377 None:intergenic 35.0%
AGAAATGGAGAAACTCAATC+TGG - chr8_4:6050561-6050580 Msa1309570:intron 35.0%
! TAGACTGTATGGGTTTTTGT+TGG - chr8_4:6051139-6051158 Msa1309570:CDS 35.0%
CGAGATTATGGACAGATAAT+TGG - chr8_4:6051274-6051293 Msa1309570:CDS 35.0%
CTGTTTGTTCACAAAAAACC+CGG + chr8_4:6051416-6051435 None:intergenic 35.0%
ATTTACTCGTTCATCTACCT+TGG + chr8_4:6051476-6051495 None:intergenic 35.0%
! TTTCGAATTCCTGTTTTGTG+AGG - chr8_4:6051595-6051614 Msa1309570:CDS 35.0%
TTACAACAATAGCTGGGTTA+AGG + chr8_4:6051895-6051914 None:intergenic 35.0%
!! CTTTAACTGACTTGGAAATG+AGG - chr8_4:6051971-6051990 Msa1309570:five_prime_UTR 35.0%
AATAGGGTTAGGGATTTGGA+GGG + chr8_4:6048379-6048398 None:intergenic 40.0%
GAAATTGGGGAATAGGGTTA+GGG + chr8_4:6048389-6048408 None:intergenic 40.0%
TGAAATTGGGGAATAGGGTT+AGG + chr8_4:6048390-6048409 None:intergenic 40.0%
AGAAGAAAGAGTGAGTAGGT+GGG + chr8_4:6048567-6048586 None:intergenic 40.0%
AAGAAGAAAGAGTGAGTAGG+TGG + chr8_4:6048568-6048587 None:intergenic 40.0%
GGAAAGACAGAAGAAAAGTG+CGG + chr8_4:6048620-6048639 None:intergenic 40.0%
CGGTGGTGATTTGAATTTGA+GGG + chr8_4:6048641-6048660 None:intergenic 40.0%
ACGGTGGTGATTTGAATTTG+AGG + chr8_4:6048642-6048661 None:intergenic 40.0%
TGTTTCACTGTGAGGACTAT+CGG + chr8_4:6048673-6048692 None:intergenic 40.0%
AGGGTTAGGGTTTATGCAAA+TGG - chr8_4:6048763-6048782 Msa1309570:intron 40.0%
! GCAAATGGTGATGTGTATGA+AGG - chr8_4:6048778-6048797 Msa1309570:intron 40.0%
TGGAAGATATGAAGGGGATT+GGG - chr8_4:6048855-6048874 Msa1309570:intron 40.0%
!! TGGGTTGATGGGAAATATGA+AGG - chr8_4:6048874-6048893 Msa1309570:intron 40.0%
!!! AAGGTTTTGGTGTTGAGAGT+TGG - chr8_4:6048893-6048912 Msa1309570:intron 40.0%
GAGTAAGGGTAGTAGGTATA+AGG - chr8_4:6048915-6048934 Msa1309570:intron 40.0%
!! GATGGATTGAGAAATGGGTT+TGG - chr8_4:6048949-6048968 Msa1309570:intron 40.0%
TATACTGGGGATGTTTATGC+TGG - chr8_4:6048985-6049004 Msa1309570:intron 40.0%
TATGCTGGTGAATGGTTGAA+TGG - chr8_4:6049000-6049019 Msa1309570:intron 40.0%
GGTGTTCATACTTGTGATGA+TGG - chr8_4:6049039-6049058 Msa1309570:intron 40.0%
!! GATGATGGGAGTAGATTTGT+TGG - chr8_4:6049054-6049073 Msa1309570:intron 40.0%
CTACTGATATCCACAAACAC+AGG + chr8_4:6049454-6049473 None:intergenic 40.0%
AGTTTGGGCCAAATATTAGG+TGG - chr8_4:6049481-6049500 Msa1309570:CDS 40.0%
! TTGACATGTTTAGGGCTTGT+AGG - chr8_4:6049688-6049707 Msa1309570:CDS 40.0%
CAGATGGAGTTTCTAAACTG+CGG - chr8_4:6049736-6049755 Msa1309570:intron 40.0%
AAACTCAGCATTAGAACCAC+AGG + chr8_4:6049838-6049857 None:intergenic 40.0%
TGCTTTATGCCTCTTCAAAC+TGG - chr8_4:6049869-6049888 Msa1309570:intron 40.0%
TCTCTTAAGTTCCTTGCAAG+AGG - chr8_4:6049909-6049928 Msa1309570:intron 40.0%
AGGTTACAAGTTCACAGTCA+TGG + chr8_4:6049941-6049960 None:intergenic 40.0%
GAAGTTGTTGCTATGTGATC+TGG + chr8_4:6049964-6049983 None:intergenic 40.0%
AGTATCAGTGACTAGGATTC+TGG - chr8_4:6050191-6050210 Msa1309570:intron 40.0%
AGAAATGGAGATACGTATGC+TGG - chr8_4:6050423-6050442 Msa1309570:intron 40.0%
GTATGCTGGTGAGTATATTG+CGG - chr8_4:6050437-6050456 Msa1309570:intron 40.0%
TATATTGCGGACAAGATGCA+TGG - chr8_4:6050450-6050469 Msa1309570:intron 40.0%
! GGGGTATACTGTTTTGCAAA+TGG - chr8_4:6050477-6050496 Msa1309570:intron 40.0%
CAATCTGGTCACTGGAAAAA+TGG - chr8_4:6050576-6050595 Msa1309570:intron 40.0%
CTGCACTGTATTAAGTACTC+TGG + chr8_4:6050667-6050686 None:intergenic 40.0%
! CAGAGTACTTAATACAGTGC+AGG - chr8_4:6050665-6050684 Msa1309570:intron 40.0%
TATTCTTCACTGAAACCACG+AGG - chr8_4:6051055-6051074 Msa1309570:CDS 40.0%
ATTCTTCACTGAAACCACGA+GGG - chr8_4:6051056-6051075 Msa1309570:CDS 40.0%
AGACTGTTCAGTCAAAACTC+TGG + chr8_4:6051120-6051139 None:intergenic 40.0%
ACTGAACAGTCTAGACTGTA+TGG - chr8_4:6051128-6051147 Msa1309570:CDS 40.0%
CTGAACAGTCTAGACTGTAT+GGG - chr8_4:6051129-6051148 Msa1309570:CDS 40.0%
! GGTTTTTGTTGGAAAGAGAG+TGG - chr8_4:6051150-6051169 Msa1309570:CDS 40.0%
TTTGTTGGAAAGAGAGTGGT+AGG - chr8_4:6051154-6051173 Msa1309570:CDS 40.0%
!! ATGGCATGCGTATTGACTAT+TGG + chr8_4:6051194-6051213 None:intergenic 40.0%
!!! CGGGTTTTTTGTGAACAAAC+AGG - chr8_4:6051414-6051433 Msa1309570:CDS 40.0%
ATCTACCTTGGCTACATCAT+AGG + chr8_4:6051464-6051483 None:intergenic 40.0%
!! AGTTGATAGCCTCACAAAAC+AGG + chr8_4:6051607-6051626 None:intergenic 40.0%
AGATTTGTAGCTAGCTACTC+TGG - chr8_4:6051667-6051686 Msa1309570:CDS 40.0%
! TATGGGTCTTGTATAGAGAG+AGG - chr8_4:6051755-6051774 Msa1309570:CDS 40.0%
! ATGGGTCTTGTATAGAGAGA+GGG - chr8_4:6051756-6051775 Msa1309570:CDS 40.0%
TCATGTAGAGTCGTATATGC+TGG - chr8_4:6051856-6051875 Msa1309570:CDS 40.0%
GGGATCTTACAACAATAGCT+GGG + chr8_4:6051901-6051920 None:intergenic 40.0%
TGGGATCTTACAACAATAGC+TGG + chr8_4:6051902-6051921 None:intergenic 40.0%
GAATAGGGTTAGGGATTTGG+AGG + chr8_4:6048380-6048399 None:intergenic 45.0%
GAAGGAGGATTAGACGGAAA+CGG + chr8_4:6048513-6048532 None:intergenic 45.0%
GGGAAAGAAGGAGGATTAGA+CGG + chr8_4:6048519-6048538 None:intergenic 45.0%
AGAAGAAAGGGAGGGAAAGA+AGG + chr8_4:6048531-6048550 None:intergenic 45.0%
GAAGAAAGAGTGAGTAGGTG+GGG + chr8_4:6048566-6048585 None:intergenic 45.0%
! AAGACAGAAGAAAAGTGCGG+CGG + chr8_4:6048617-6048636 None:intergenic 45.0%
TAGTCCTCACAGTGAAACAG+TGG - chr8_4:6048674-6048693 Msa1309570:CDS 45.0%
GATTCCACTGTTTCACTGTG+AGG + chr8_4:6048681-6048700 None:intergenic 45.0%
CAGTGAAACAGTGGAATCGA+CGG - chr8_4:6048683-6048702 Msa1309570:CDS 45.0%
!! AATGAGAAGTGTTGTGGGAG+TGG - chr8_4:6048811-6048830 Msa1309570:intron 45.0%
!! ATGAGAAGTGTTGTGGGAGT+GGG - chr8_4:6048812-6048831 Msa1309570:intron 45.0%
GTGGAAGATATGAAGGGGAT+TGG - chr8_4:6048854-6048873 Msa1309570:intron 45.0%
!! TATGAAGGGGATTGGGTTGA+TGG - chr8_4:6048862-6048881 Msa1309570:intron 45.0%
!! ATGAAGGGGATTGGGTTGAT+GGG - chr8_4:6048863-6048882 Msa1309570:intron 45.0%
! TGGTGTTGAGAGTTGGAGTA+AGG - chr8_4:6048900-6048919 Msa1309570:intron 45.0%
! GGTGTTGAGAGTTGGAGTAA+GGG - chr8_4:6048901-6048920 Msa1309570:intron 45.0%
AGAGTTGGAGTAAGGGTAGT+AGG - chr8_4:6048908-6048927 Msa1309570:intron 45.0%
GGGATGTTTATGCTGGTGAA+TGG - chr8_4:6048992-6049011 Msa1309570:intron 45.0%
TGGTTGAATGGTCAGAGTCA+TGG - chr8_4:6049012-6049031 Msa1309570:intron 45.0%
GGTTGAATGGTCAGAGTCAT+GGG - chr8_4:6049013-6049032 Msa1309570:intron 45.0%
! TGGGGTGTTAAACATGGTCT+TGG - chr8_4:6049087-6049106 Msa1309570:intron 45.0%
GTCTTGGCCACTATCACTTT+AGG - chr8_4:6049103-6049122 Msa1309570:intron 45.0%
CAATTCAAGAACACCGGACA+CGG + chr8_4:6049311-6049330 None:intergenic 45.0%
ATGTTTCACCAGTTCCTAGC+TGG - chr8_4:6049644-6049663 Msa1309570:CDS 45.0%
GCTTTCAGACCAGTTTGAAG+AGG + chr8_4:6049881-6049900 None:intergenic 45.0%
CATGGACTTAACCTCTTGCA+AGG + chr8_4:6049923-6049942 None:intergenic 45.0%
GTTGTTGCTATGTGATCTGG+AGG + chr8_4:6049961-6049980 None:intergenic 45.0%
! AGTGTTGCTGATGGTCAACA+AGG + chr8_4:6050124-6050143 None:intergenic 45.0%
GTTGGTCAGTATCAGTGACT+AGG - chr8_4:6050184-6050203 Msa1309570:intron 45.0%
GCAAATGGCCATCGTTATGA+AGG - chr8_4:6050492-6050511 Msa1309570:intron 45.0%
TTATGAAGGAACCTGGCATG+AGG - chr8_4:6050506-6050525 Msa1309570:intron 45.0%
TATGAAGGAACCTGGCATGA+GGG - chr8_4:6050507-6050526 Msa1309570:intron 45.0%
GAGAAACTCAATCTGGTCAC+TGG - chr8_4:6050568-6050587 Msa1309570:intron 45.0%
AACAGGGGAAACTGGATATG+TGG + chr8_4:6050631-6050650 None:intergenic 45.0%
ACATATCCAGTTTCCCCTGT+TGG - chr8_4:6050630-6050649 Msa1309570:intron 45.0%
TGGAGTGATTGACACCAACA+GGG + chr8_4:6050647-6050666 None:intergenic 45.0%
TTCTTCACTGAAACCACGAG+GGG - chr8_4:6051057-6051076 Msa1309570:CDS 45.0%
GAAAGCCTATGATGTAGCCA+AGG - chr8_4:6051456-6051475 Msa1309570:CDS 45.0%
TTTGTAGCTAGCTACTCTGG+TGG - chr8_4:6051670-6051689 Msa1309570:CDS 45.0%
GTCTTGTATAGAGAGAGGGA+AGG - chr8_4:6051760-6051779 Msa1309570:CDS 45.0%
TCAAAACATCTTGCGCACGA+TGG + chr8_4:6051945-6051964 None:intergenic 45.0%
!!! TTTTTTTTTTAAATTACTAA+TGG - chr8_4:6049269-6049288 Msa1309570:intron 5.0%
GGGGAATAGGGTTAGGGATT+TGG + chr8_4:6048383-6048402 None:intergenic 50.0%
AGAAAGGGAGGGAAAGAAGG+AGG + chr8_4:6048528-6048547 None:intergenic 50.0%
CGGAGAAAGCGAAAGCAGAT+AGG + chr8_4:6048597-6048616 None:intergenic 50.0%
AGGACTATCGGAAGAGACGA+CGG + chr8_4:6048661-6048680 None:intergenic 50.0%
TGAAACAGTGGAATCGACGG+CGG - chr8_4:6048686-6048705 Msa1309570:CDS 50.0%
!! TGAGAAGTGTTGTGGGAGTG+GGG - chr8_4:6048813-6048832 Msa1309570:intron 50.0%
AATGGTCAGAGTCATGGGAG+TGG - chr8_4:6049018-6049037 Msa1309570:intron 50.0%
TTTCACCAGTTCCTAGCTGG+TGG - chr8_4:6049647-6049666 Msa1309570:CDS 50.0%
! GGGTGTATCAGTGTTGCTGA+TGG + chr8_4:6050133-6050152 None:intergenic 50.0%
GCGGACAAGATGCATGGATT+CGG - chr8_4:6050456-6050475 Msa1309570:intron 50.0%
CGGACAAGATGCATGGATTC+GGG - chr8_4:6050457-6050476 Msa1309570:intron 50.0%
GGACAAGATGCATGGATTCG+GGG - chr8_4:6050458-6050477 Msa1309570:intron 50.0%
GCCATCGTTATGAAGGAACC+TGG - chr8_4:6050499-6050518 Msa1309570:intron 50.0%
GCCAGGTTCCTTCATAACGA+TGG + chr8_4:6050503-6050522 None:intergenic 50.0%
ATGAAGGAACCTGGCATGAG+GGG - chr8_4:6050508-6050527 Msa1309570:intron 50.0%
! ATGTCTTTTCCCCTCATGCC+AGG + chr8_4:6050520-6050539 None:intergenic 50.0%
TGGCATGAGGGGAAAAGACA+TGG - chr8_4:6050519-6050538 Msa1309570:intron 50.0%
GAGGGGAAAAGACATGGTCT+TGG - chr8_4:6050525-6050544 Msa1309570:intron 50.0%
TTGACACCAACAGGGGAAAC+TGG + chr8_4:6050639-6050658 None:intergenic 50.0%
GGAGTGATTGACACCAACAG+GGG + chr8_4:6050646-6050665 None:intergenic 50.0%
CTGGAGTGATTGACACCAAC+AGG + chr8_4:6050648-6050667 None:intergenic 50.0%
TCTTCACTGAAACCACGAGG+GGG - chr8_4:6051058-6051077 Msa1309570:CDS 50.0%
GTCAGCAACTGTGGAGATGA+TGG + chr8_4:6051705-6051724 None:intergenic 50.0%
TTGCGTTCAGTCAGCAACTG+TGG + chr8_4:6051714-6051733 None:intergenic 50.0%
AGTCGTATATGCTGGTGTGC+TGG - chr8_4:6051864-6051883 Msa1309570:CDS 50.0%
GGATTAGACGGAAACGGTGG+CGG + chr8_4:6048507-6048526 None:intergenic 55.0%
GGAGGATTAGACGGAAACGG+TGG + chr8_4:6048510-6048529 None:intergenic 55.0%
ACTATCGGAAGAGACGACGG+TGG + chr8_4:6048658-6048677 None:intergenic 55.0%
TATGTGGCGTTCTGCGTGCT+TGG + chr8_4:6050615-6050634 None:intergenic 55.0%
CTTCACTGAAACCACGAGGG+GGG - chr8_4:6051059-6051078 Msa1309570:CDS 55.0%
TTCACTGAAACCACGAGGGG+GGG - chr8_4:6051060-6051079 Msa1309570:CDS 55.0%
AGCAGCTACTCTGGCTGCAT+TGG + chr8_4:6051527-6051546 None:intergenic 55.0%
AGCCAGAGTAGCTGCTGTCA+AGG - chr8_4:6051531-6051550 Msa1309570:CDS 55.0%
AGCCTTGACAGCAGCTACTC+TGG + chr8_4:6051536-6051555 None:intergenic 55.0%
ATCTGGGTCAGTGCCGTGTC+CGG - chr8_4:6049295-6049314 Msa1309570:intron 60.0%
ACCAGTTCCTAGCTGGTGGC+TGG - chr8_4:6049651-6049670 Msa1309570:CDS 60.0%
TTGTTCTGTTCCCCCCCTCG+TGG + chr8_4:6051073-6051092 None:intergenic 60.0%
GAGTGCGGCGGATGAGATGG+CGG + chr8_4:6048476-6048495 None:intergenic 65.0%
!! GACGGAAACGGTGGCGGAGA+CGG + chr8_4:6048501-6048520 None:intergenic 65.0%
GCCAGCCACCAGCTAGGAAC+TGG + chr8_4:6049655-6049674 None:intergenic 65.0%
GTTCCTAGCTGGTGGCTGGC+TGG - chr8_4:6049655-6049674 Msa1309570:CDS 65.0%
GCGGAGTGCGGCGGATGAGA+TGG + chr8_4:6048479-6048498 None:intergenic 70.0%
!! GGAAACGGTGGCGGAGACGG+CGG + chr8_4:6048498-6048517 None:intergenic 70.0%
CAGCCAGCCAGCCACCAGCT+AGG + chr8_4:6049661-6049680 None:intergenic 70.0%
! CTAGCTGGTGGCTGGCTGGC+TGG - chr8_4:6049659-6049678 Msa1309570:CDS 70.0%
! CTGGTGGCTGGCTGGCTGGT+TGG - chr8_4:6049663-6049682 Msa1309570:CDS 70.0%
! GTGGCGGAGACGGCGGAGTG+CGG + chr8_4:6048491-6048510 None:intergenic 75.0%
! GCGGAGACGGCGGAGTGCGG+CGG + chr8_4:6048488-6048507 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 6048125 6052032 6048125 ID=Msa1309570;Name=Msa1309570
chr8_4 mRNA 6048125 6052032 6048125 ID=Msa1309570-mRNA-1;Parent=Msa1309570;Name=Msa1309570-mRNA-1;_AED=0.02;_eAED=0.02;_QI=86|1|1|1|1|1|3|417|451
chr8_4 exon 6048125 6048721 6048125 ID=Msa1309570-mRNA-1:exon:2044;Parent=Msa1309570-mRNA-1
chr8_4 exon 6049471 6049732 6049471 ID=Msa1309570-mRNA-1:exon:2043;Parent=Msa1309570-mRNA-1
chr8_4 exon 6051033 6052032 6051033 ID=Msa1309570-mRNA-1:exon:2042;Parent=Msa1309570-mRNA-1
chr8_4 five_prime_UTR 6051947 6052032 6051947 ID=Msa1309570-mRNA-1:five_prime_utr;Parent=Msa1309570-mRNA-1
chr8_4 CDS 6051033 6051946 6051033 ID=Msa1309570-mRNA-1:cds;Parent=Msa1309570-mRNA-1
chr8_4 CDS 6049471 6049732 6049471 ID=Msa1309570-mRNA-1:cds;Parent=Msa1309570-mRNA-1
chr8_4 CDS 6048542 6048721 6048542 ID=Msa1309570-mRNA-1:cds;Parent=Msa1309570-mRNA-1
chr8_4 three_prime_UTR 6048125 6048541 6048125 ID=Msa1309570-mRNA-1:three_prime_utr;Parent=Msa1309570-mRNA-1
Gene Sequence

>Msa1309570

ATGCATCAGAAAAAATCAGAAATTCAGATCGGAAAAGAAAGTTCTTGTATCTCTTCCGATTTCAACCCTAATCTCTTTCATCCTCATAAACTTTTCAATTCAATTCACCACAAAAACCAACACAATTCTATTTCTTCACCAATTCATCATAAAATCAAAATCAAACCCTCCAAATCCCTAACCCTATTCCCCAATTTCAATTTCAATTTCAATTTCCCACAATTTTCCACTAAAAAATCATCTTTTCATTTTCATTCTCTCTCCGCCATCTCATCCGCCGCACTCCGCCGTCTCCGCCACCGTTTCCGTCTAATCCTCCTTCTTTCCCTCCCTTTCTTCTTCTTTTTACTATCCCCACCTACTCACTCTTTCTTCTTTAATTTCCTATCTGCTTTCGCTTTCTCCGCCGCACTTTTCTTCTGTCTTTCCCTCAAATTCAAATCACCACCGTCGTCTCTTCCGATAGTCCTCACAGTGAAACAGTGGAATCGACGGCGGAGAGAGAATAAAATTTCCAAAAATTCGAAAAATTCATCCAATATTGTGGAAATTAGGGTTAGGGTTTATGCAAATGGTGATGTGTATGAAGGTGAAATGAAGAATGAGAAGTGTTGTGGGAGTGGGGTTTATTACTATAATATGAGTGGAAGATATGAAGGGGATTGGGTTGATGGGAAATATGAAGGTTTTGGTGTTGAGAGTTGGAGTAAGGGTAGTAGGTATAAGGGTATGTATAGAGATGGATTGAGAAATGGGTTTGGTGTTTATAGGTTTTATACTGGGGATGTTTATGCTGGTGAATGGTTGAATGGTCAGAGTCATGGGAGTGGTGTTCATACTTGTGATGATGGGAGTAGATTTGTTGGTGAGTTTAAGTGGGGTGTTAAACATGGTCTTGGCCACTATCACTTTAGAAATGGAGATACGTATGCTGGTGAGTATATTGCGGACAAGATGCATGGATTCGGGGTATACTGTTTTGCAAATGGCCATCGTTATGAAGGAACCTGGCATGAGGGGAAAAGACATGGTCTTGGAATGTATACATTTAGAAATGGAGAAACTCAATCTGGTCACTGGAAAAATGGAGTACTTGACATTCCAAGCACGCAGAACGCCACATATCCAGTTTCCCCTGTTGGTGTCAATCACTCCAGAGTACTTAATACAGTGCAGGAAGCTAGAAGAGCAGCTGAGAAAGCCTATGATGTAGCCAAGGTAGATGAACGAGTAAATAGATCAGTATCAGCAGCTAATAGAGCAGCCAATGCAGCCAGAGTAGCTGCTGTCAAGGCTGTGCAAAATGAAATGCATCATCATGTTAACGAAGAGAGCTTTCGAATTCCTGTTTTGTGA
Protein sequence

>Msa1309570

MHQKKSEIQIGKESSCISSDFNPNLFHPHKLFNSIHHKNQHNSISSPIHHKIKIKPSKSLTLFPNFNFNFNFPQFSTKKSSFHFHSLSAISSAALRRLRHRFRLILLLSLPFFFFLLSPPTHSFFFNFLSAFAFSAALFFCLSLKFKSPPSSLPIVLTVKQWNRRRRENKISKNSKNSSNIVEIRVRVYANGDVYEGEMKNEKCCGSGVYYYNMSGRYEGDWVDGKYEGFGVESWSKGSRYKGMYRDGLRNGFGVYRFYTGDVYAGEWLNGQSHGSGVHTCDDGSRFVGEFKWGVKHGLGHYHFRNGDTYAGEYIADKMHGFGVYCFANGHRYEGTWHEGKRHGLGMYTFRNGETQSGHWKNGVLDIPSTQNATYPVSPVGVNHSRVLNTVQEARRAAEKAYDVAKVDERVNRSVSAANRAANAARVAAVKAVQNEMHHHVNEESFRIPVL*