Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0363060 | XP_013460772.1 | 96.154 | 494 | 19 | 0 | 1 | 494 | 1 | 494 | 0.0 | 986 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0363060 | sp|Q54ND5|MINP1_DICDI | 27.174 | 460 | 259 | 10 | 56 | 462 | 102 | 538 | 1.94e-48 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0363060 | A0A072UZE6 | 96.154 | 494 | 19 | 0 | 1 | 494 | 1 | 494 | 0.0 | 986 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000880 | Msa0363060 | 0.812522 | 4.063474e-51 | -8.615850e-47 |
| Msa0000910 | Msa0363060 | 0.850157 | 2.035439e-60 | -8.615850e-47 |
| Msa0002560 | Msa0363060 | 0.873217 | 1.744982e-67 | -8.615850e-47 |
| Msa0002680 | Msa0363060 | 0.847878 | 8.752049e-60 | -8.615850e-47 |
| Msa0002710 | Msa0363060 | 0.807355 | 5.264369e-50 | -8.615850e-47 |
| Msa0003220 | Msa0363060 | 0.877034 | 8.682129e-69 | -8.615850e-47 |
| Msa0003270 | Msa0363060 | 0.861127 | 1.275893e-63 | -8.615850e-47 |
| Msa0003630 | Msa0363060 | 0.811316 | 7.441183e-51 | -8.615850e-47 |
| Msa0004540 | Msa0363060 | 0.823623 | 1.253473e-53 | -8.615850e-47 |
| Msa0005270 | Msa0363060 | 0.810025 | 1.415260e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0363060 | MtrunA17_Chr3g0114041 | 95.910 | 489 | 20 | 0 | 1 | 489 | 1 | 489 | 0.0 | 946 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0363060 | AT1G09870.1 | 66.049 | 486 | 151 | 4 | 2 | 482 | 3 | 479 | 0.0 | 678 |
Find 139 sgRNAs with CRISPR-Local
Find 348 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGGCGGGGCAGCACCG+TGG | 0.668105 | 3_1:-55141206 | Msa0363060:CDS |
| AAACCAAGCAGACATGAGAA+TGG | 0.520129 | 3_1:+55141411 | None:intergenic |
| AAAGAAAAGGATAAGGGAGT+TGG | 0.492650 | 3_1:-55144456 | Msa0363060:CDS |
| AAAGCTTACCGGCATTGGAA+TGG | 0.488189 | 3_1:+55141084 | None:intergenic |
| AAATCACTAAATTATAGAAT+GGG | 0.395225 | 3_1:-55142454 | Msa0363060:CDS |
| AACAAACTAAAATTCATCTT+TGG | 0.224993 | 3_1:+55140432 | None:intergenic |
| AACATCTCTCCACCGTGTCC+AGG | 0.481498 | 3_1:-55145485 | Msa0363060:intron |
| AACCAAGCAGACATGAGAAT+GGG | 0.505960 | 3_1:+55141412 | None:intergenic |
| AAGCTTACCGGCATTGGAAT+GGG | 0.404546 | 3_1:+55141085 | None:intergenic |
| AATCACTAAATTATAGAATG+GGG | 0.619098 | 3_1:-55142453 | Msa0363060:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTAACAGAACAAGAGTGAT+TGG | + | chr3_1:55140086-55140105 | None:intergenic | 30.0% | |
| GAATGTTGGAAAACATGTTA+GGG | + | chr3_1:55140198-55140217 | None:intergenic | 30.0% | |
| AGAATGTTGGAAAACATGTT+AGG | + | chr3_1:55140199-55140218 | None:intergenic | 30.0% | |
| ACTAATATAGACATGGACAT+TGG | + | chr3_1:55140560-55140579 | None:intergenic | 30.0% | |
| ATTTATGACTGTTGAAACGA+TGG | - | chr3_1:55140656-55140675 | Msa0363060:intron | 30.0% | |
| TTTATGACTGTTGAAACGAT+GGG | - | chr3_1:55140657-55140676 | Msa0363060:intron | 30.0% | |
| GTGTATTAGGCTGTTATATT+GGG | - | chr3_1:55140848-55140867 | Msa0363060:intron | 30.0% | |
| CTAATCACTTCTATACCAAA+TGG | - | chr3_1:55141106-55141125 | Msa0363060:CDS | 30.0% | |
| TGGAATAGTATGATCATTGA+TGG | - | chr3_1:55141126-55141145 | Msa0363060:CDS | 30.0% | |
| TCCAACAAAGAAAAGGATAA+GGG | - | chr3_1:55141188-55141207 | Msa0363060:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 55139998 | 55145674 | 55139998 | ID=Msa0363060;Name=Msa0363060 |
| chr3_1 | mRNA | 55139998 | 55145674 | 55139998 | ID=Msa0363060-mRNA-1;Parent=Msa0363060;Name=Msa0363060-mRNA-1;_AED=0.17;_eAED=0.17;_QI=76|1|1|1|0.90|0.83|12|419|494 |
| chr3_1 | exon | 55139998 | 55140233 | 55139998 | ID=Msa0363060-mRNA-1:exon:10018;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55140255 | 55140587 | 55140255 | ID=Msa0363060-mRNA-1:exon:10017;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55140695 | 55140736 | 55140695 | ID=Msa0363060-mRNA-1:exon:10016;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55141093 | 55141289 | 55141093 | ID=Msa0363060-mRNA-1:exon:10015;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55141394 | 55141498 | 55141394 | ID=Msa0363060-mRNA-1:exon:10014;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55142395 | 55142533 | 55142395 | ID=Msa0363060-mRNA-1:exon:10013;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55142890 | 55142946 | 55142890 | ID=Msa0363060-mRNA-1:exon:10012;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55143087 | 55143227 | 55143087 | ID=Msa0363060-mRNA-1:exon:10011;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55143784 | 55143942 | 55143784 | ID=Msa0363060-mRNA-1:exon:10010;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55144211 | 55144504 | 55144211 | ID=Msa0363060-mRNA-1:exon:10009;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55145301 | 55145388 | 55145301 | ID=Msa0363060-mRNA-1:exon:10008;Parent=Msa0363060-mRNA-1 |
| chr3_1 | exon | 55145486 | 55145674 | 55145486 | ID=Msa0363060-mRNA-1:exon:10007;Parent=Msa0363060-mRNA-1 |
| chr3_1 | five_prime_UTR | 55145599 | 55145674 | 55145599 | ID=Msa0363060-mRNA-1:five_prime_utr;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55145486 | 55145598 | 55145486 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55145301 | 55145388 | 55145301 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55144211 | 55144504 | 55144211 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55143784 | 55143942 | 55143784 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55143087 | 55143227 | 55143087 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55142890 | 55142946 | 55142890 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55142395 | 55142533 | 55142395 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55141394 | 55141498 | 55141394 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55141093 | 55141289 | 55141093 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55140695 | 55140736 | 55140695 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | CDS | 55140438 | 55140587 | 55140438 | ID=Msa0363060-mRNA-1:cds;Parent=Msa0363060-mRNA-1 |
| chr3_1 | three_prime_UTR | 55140255 | 55140437 | 55140255 | ID=Msa0363060-mRNA-1:three_prime_utr;Parent=Msa0363060-mRNA-1 |
| chr3_1 | three_prime_UTR | 55139998 | 55140233 | 55139998 | ID=Msa0363060-mRNA-1:three_prime_utr;Parent=Msa0363060-mRNA-1 |
| Gene Sequence |
>Msa0363060 ATGGCGAAACCAATCACTCTTGTTCTGTTAATATTATTCCTTTCAATTTTACATTTCTCAAACGCTGCAGAAGAAGCTTTTGATGTTCGCAAACATCTCTCCACCGTGTCCAGGTACGGAGTTGTGAAAGACATTACTGACAAGAATTTCATTCCTTCTAAAATTCCTGAAGGATGTACTCCAATTCATTTAAATCTTGTGGCAAGGCATGGAACTCGTTCTCCAACAAAGAAAAGGATAAGGGAGTTGGATAATTTGGCAGCTCAATTGGAAGATCTTGTAAGGGATGCGAAAGAGCGGAAATTGTCTTTGGAGAGAGTTCCGTCTTGGTTAAATGGATGGAAATCTCCTTGGCATGGAAGGCTTAAGGGCGGTGAGTTAATTAGACTAGGAGAGGAAGAATTGTTTGAACTTGGAATCAGGATTAGAGAAAGGTTTCCGAGTTTGTTTGATGAGGACTACCATCCAGACATATATCCTATAAAGGCAACTCAGGTTCCCCGAGCATCAGCTAGTGCTGTGGCATTTGGAATGGGTCTTTTCAGTGGCAATGGAACTCTTGGAACTGGTAAACACAGAGCTTTTGCTGTTGCAAGTGAAAGCCGTGCTAGTGACATCATGCTGAGATTTCATGATTGTTGTCATAACTACAAGGATTTCAGGAAAAAGAAGGAACCCGCAGTTGATAAACTTAAGGAACCTATCTTGGATGAGATTACATCTGCATTAGTTGGGCGATATGGGCTGAATTTTACAAGGCAGATTACATCATCTCTCTGGTTCTTATGTAAACAGGAAGCATCCTTGTTGGATATAACTGATCAAGCGTGTAGTCTTTTCAGCCCATCGGAGATTACATTGCTGGAGTGGACAGATGACTTGGAGGCGTTTATTCTGAAGGGTTATGGTAAATCACTAAATTATAGAATGGGGATGCCATTACTTGAAGATGTTGTTCAATCCATGGAGCAAGCTATTAAAGCTGAAGAAGAAAAACATGTTCCTGGCAGCTTTGAAAAAGCAAGACTCCGGTTTGCTCATGCAGAAACTATAGTCCCATTCTCATGTCTGCTTGGTTTATTTCTTGAAGAGTCTGAGTTTGACAAAATCCAGAAGGAGAAACCTTTGGAGCTACCTCCAAAGCCTCCACAGAAAAGAAAATGGCGGGGCAGCACCGTGGCTCCTTTTGCTGGTAACAACATGCTGCTCCTGTATAGTTGTCCTGAGTCAGGCAAAGCTAAAAGCAAGCACTTCGTGCAAGTGCTGCATAATGAACACCCCATTCCAATGCCGGGTTGTCATGGTTCTGATTTCTGTCCGTTTGAAGTATTCAAGGAAAAAATAGTTGCACCTCATCGGAAGCATGACTATGATACAGTTTGTAATGCAAAGCAAGAGCCGAAGTCCCCACGGAGTATGATATTCCAAATATTTCAGTGGCTTTTCCCACTAGCGAAAGGTGACAACCCCAAAGATGAATTTTAG |
| Protein sequence |
>Msa0363060 MAKPITLVLLILFLSILHFSNAAEEAFDVRKHLSTVSRYGVVKDITDKNFIPSKIPEGCTPIHLNLVARHGTRSPTKKRIRELDNLAAQLEDLVRDAKERKLSLERVPSWLNGWKSPWHGRLKGGELIRLGEEELFELGIRIRERFPSLFDEDYHPDIYPIKATQVPRASASAVAFGMGLFSGNGTLGTGKHRAFAVASESRASDIMLRFHDCCHNYKDFRKKKEPAVDKLKEPILDEITSALVGRYGLNFTRQITSSLWFLCKQEASLLDITDQACSLFSPSEITLLEWTDDLEAFILKGYGKSLNYRMGMPLLEDVVQSMEQAIKAEEEKHVPGSFEKARLRFAHAETIVPFSCLLGLFLEESEFDKIQKEKPLELPPKPPQKRKWRGSTVAPFAGNNMLLLYSCPESGKAKSKHFVQVLHNEHPIPMPGCHGSDFCPFEVFKEKIVAPHRKHDYDTVCNAKQEPKSPRSMIFQIFQWLFPLAKGDNPKDEF* |
