AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1313250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1313250 MtrunA17_Chr8g0341821 93.527 448 17 2 1 436 1 448 0.0 841
Msa1313250 MtrunA17_Chr8g0339291 67.804 469 124 7 1 446 1 465 0.0 614
Msa1313250 MtrunA17_Chr7g0235071 65.875 463 128 7 1 443 1 453 0.0 602
Msa1313250 MtrunA17_Chr7g0238471 71.605 81 22 1 61 141 34 113 5.22e-37 131
Msa1313250 MtrunA17_Chr7g0238481 56.180 89 34 2 182 270 14 97 2.87e-23 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1313250 AT3G07270.1 56.629 445 169 8 21 447 23 461 3.01e-171 489
Msa1313250 AT3G07270.2 56.629 445 169 8 21 447 23 461 3.04e-171 489

Find 96 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACATTGCACAAATTTA+AGG 0.111527 8_4:+11096090 None:intergenic
AGTACTGATTGCTTTAATTT+TGG 0.156856 8_4:+11096795 None:intergenic
AATGACAAAGTTCGTCTTAA+AGG 0.202003 8_4:-11096865 Msa1313250:CDS
TCTTCAATTGTTAAATCTTT+AGG 0.230854 8_4:-11096751 Msa1313250:CDS
TATGTGAACAACACCATAAA+AGG 0.261763 8_4:+11096525 None:intergenic
GGCCTAAATAATAATAAAAT+TGG 0.265269 8_4:-11097303 Msa1313250:CDS
TGCTGCAAGGACCAAGTTCT+TGG 0.279067 8_4:-11096248 Msa1313250:CDS
AAAGATCTGTTGCTAGGTTT+GGG 0.298958 8_4:-11099135 Msa1313250:CDS
TATACAATCCATGGCCATTA+TGG 0.301148 8_4:+11095899 None:intergenic
CAAAGATCTGTTGCTAGGTT+TGG 0.317637 8_4:-11099136 Msa1313250:CDS
TTATTATTTAGGCCATCTTC+AGG 0.323009 8_4:+11097312 None:intergenic
TCTACAAGCTCTTTCCTTGT+TGG 0.323847 8_4:+11096721 None:intergenic
GAAACTGCATTGACCATAAT+AGG 0.333087 8_4:+11096772 None:intergenic
GTTAATCTAATTGTTTCTTC+AGG 0.336586 8_4:-11096964 Msa1313250:CDS
TGCTTAAGCCTACCACTCTT+TGG 0.341064 8_4:+11097163 None:intergenic
CTAATTGTTTCTTCAGGATC+TGG 0.341640 8_4:-11096958 Msa1313250:CDS
TCTAGGGGAATTGAGAAGTT+TGG 0.342366 8_4:-11096319 Msa1313250:CDS
CATGTTGGTTATATCCCTTC+AGG 0.349179 8_4:-11097186 Msa1313250:CDS
GATATCGACTCAAACGATAT+AGG 0.353026 8_4:-11096991 Msa1313250:CDS
ATGAACTATTGATTGCAAAA+TGG 0.356937 8_4:+11096462 None:intergenic
TGCAAAATGGACTTTCCAAT+TGG 0.376372 8_4:+11096475 None:intergenic
ACATGACACTTGACTTGAAA+TGG 0.378614 8_4:+11097204 None:intergenic
AGTATGATAGCAACTCCTTC+TGG 0.380673 8_4:+11097045 None:intergenic
TGCTCACATTGTGACCAAAA+AGG 0.383650 8_4:+11096559 None:intergenic
AGCAATCAGTACTCCTATTA+TGG 0.386350 8_4:-11096785 Msa1313250:CDS
TCGATATCTTTGTATGGAAA+AGG 0.388798 8_4:+11097006 None:intergenic
TCCACCTACCAATGGTGAAA+TGG 0.397277 8_4:+11096384 None:intergenic
TTTAGTCTTCTTAAGTTTCG+AGG 0.398957 8_4:-11096895 Msa1313250:CDS
ATAGGATACTTTCTTTCTAA+AGG 0.402986 8_4:-11096505 Msa1313250:CDS
CCAAGTGCTACATCTCTTGA+AGG 0.424595 8_4:-11096217 Msa1313250:CDS
ATATCGACTCAAACGATATA+GGG 0.424779 8_4:-11096990 Msa1313250:CDS
AACATTGATATTGAGATGAA+TGG 0.425168 8_4:-11096649 Msa1313250:CDS
TTTGAGTCGATATCTTTGTA+TGG 0.426394 8_4:+11097000 None:intergenic
AAAACTTTGGTGTATTTGAT+TGG 0.432637 8_4:-11095952 Msa1313250:three_prime_UTR
TCTAAAGGATGCTATCCAAT+TGG 0.437570 8_4:-11096490 Msa1313250:CDS
AATAAAATTGGTCATGGTGG+TGG 0.440425 8_4:-11097291 Msa1313250:CDS
TTTCAGTTAATGGGGTCGTT+TGG 0.445001 8_4:-11099255 None:intergenic
GGCTGAAGATTTGAAAACTT+TGG 0.458466 8_4:-11095965 Msa1313250:three_prime_UTR
TCAAGCTTCAAGTTCAAGAA+AGG 0.466364 8_4:-11096429 Msa1313250:CDS
GTCACACTTGTATGATCTCT+AGG 0.476571 8_4:-11096336 Msa1313250:CDS
ATCTTTGACTGCACACTCAA+TGG 0.481435 8_4:+11099152 None:intergenic
ATTGGTCATGGTGGTGGAGT+AGG 0.493372 8_4:-11097285 Msa1313250:CDS
TGTGTTCAGCTTTGCATCAA+GGG 0.508751 8_4:-11097074 Msa1313250:CDS
CGATATCTTTGTATGGAAAA+GGG 0.509754 8_4:+11097007 None:intergenic
TGCAGAAACCATTTCACCAT+TGG 0.513288 8_4:-11096392 Msa1313250:CDS
ATGTGTTCAGCTTTGCATCA+AGG 0.516850 8_4:-11097075 Msa1313250:CDS
CTAATACCAATGGAGAGGTA+AGG 0.520921 8_4:-11096618 Msa1313250:CDS
CTACCACTCTTTGGCCTGAA+GGG 0.523240 8_4:+11097172 None:intergenic
CCTACCACTCTTTGGCCTGA+AGG 0.523497 8_4:+11097171 None:intergenic
TGGAATACTCTCCAAGAACT+TGG 0.524589 8_4:+11096237 None:intergenic
ATTCTACCACATGTCATATG+AGG 0.525458 8_4:+11096128 None:intergenic
CCTTCAAGAGATGTAGCACT+TGG 0.525506 8_4:+11096217 None:intergenic
TCCTAAAACAGCAATGGTTG+AGG 0.526006 8_4:+11096288 None:intergenic
ATGTGAACAACACCATAAAA+GGG 0.531357 8_4:+11096526 None:intergenic
TATTATTTAGGCCATCTTCA+GGG 0.537173 8_4:+11097313 None:intergenic
ATCAAACCTTACCTCTCCAT+TGG 0.540240 8_4:+11096612 None:intergenic
TCACACTTGTATGATCTCTA+GGG 0.541059 8_4:-11096335 Msa1313250:CDS
AATAATAATAAAATTGGTCA+TGG 0.542699 8_4:-11097297 Msa1313250:CDS
TATTGAACTTAATAGAAAAG+AGG 0.544660 8_4:+11095927 None:intergenic
AATAATAAAATTGGTCATGG+TGG 0.544701 8_4:-11097294 Msa1313250:CDS
CAGAAGAGATATACAATCCA+TGG 0.548438 8_4:+11095890 None:intergenic
GGTTACATTTCTAATACCAA+TGG 0.551456 8_4:-11096628 Msa1313250:CDS
GGTGCATTATTCCCTGAAGA+TGG 0.565188 8_4:-11097324 Msa1313250:CDS
GCAGTCAAAGATCTGTTGCT+AGG 0.569319 8_4:-11099141 Msa1313250:CDS
TCAAATGCATTCAAATTGTG+AGG 0.569936 8_4:-11099232 Msa1313250:CDS
CTTAAGCAAGCTTCCTCGTG+TGG 0.570483 8_4:-11097148 Msa1313250:CDS
AAGATCTGTTGCTAGGTTTG+GGG 0.575866 8_4:-11099134 Msa1313250:CDS
CCTTCAGGCCAAAGAGTGGT+AGG 0.576895 8_4:-11097171 Msa1313250:CDS
TTTCAAGTCAAGTGTCATGT+TGG 0.580286 8_4:-11097201 Msa1313250:CDS
ACCATTTCACCATTGGTAGG+TGG 0.594194 8_4:-11096385 Msa1313250:CDS
GGTCATGGTGGTGGAGTAGG+TGG 0.598165 8_4:-11097282 Msa1313250:CDS
GAAACCATTTCACCATTGGT+AGG 0.598373 8_4:-11096388 Msa1313250:CDS
GGGGAAGATATTAACAGGGA+AGG 0.599304 8_4:-11099114 Msa1313250:CDS
AGATCTGTTGCTAGGTTTGG+GGG 0.602359 8_4:-11099133 Msa1313250:CDS
AATGTGAAGGACATTGTTGA+AGG 0.604917 8_4:-11097345 Msa1313250:CDS
AAGGACCACTCGATCAATGT+TGG 0.610498 8_4:-11096846 Msa1313250:CDS
GAAACTTCAGCATATAGAGA+AGG 0.616046 8_4:+11096820 None:intergenic
AAGACTCACAATAAGAGTAG+AGG 0.617418 8_4:+11097235 None:intergenic
TCGACTCAAACGATATAGGG+TGG 0.618583 8_4:-11096987 Msa1313250:CDS
CGACTCAAACGATATAGGGT+GGG 0.620043 8_4:-11096986 Msa1313250:CDS
CAAGTGCTACATCTCTTGAA+GGG 0.625994 8_4:-11096216 Msa1313250:CDS
AAGCAAGCTTCCTCGTGTGG+CGG 0.628840 8_4:-11097145 Msa1313250:CDS
CTTTAGGAGAAGATCCAACA+AGG 0.631850 8_4:-11096735 Msa1313250:CDS
AAATGTGATTGTAGTTGTTG+AGG 0.632370 8_4:-11096362 Msa1313250:CDS
CACACTTGTATGATCTCTAG+GGG 0.636802 8_4:-11096334 Msa1313250:CDS
AGGTTATAGGCAAAATGTGA+AGG 0.638395 8_4:-11097358 Msa1313250:CDS
ATCACATTTCCACCTACCAA+TGG 0.639419 8_4:+11096376 None:intergenic
GGACACCAACATTGATCGAG+TGG 0.642099 8_4:+11096841 None:intergenic
GCAAATGCTTCCGCCACACG+AGG 0.643408 8_4:+11097135 None:intergenic
GAAGTGTCCTAAAACAGCAA+TGG 0.645074 8_4:+11096282 None:intergenic
CATTTCTAATACCAATGGAG+AGG 0.648093 8_4:-11096623 Msa1313250:CDS
AGTCTTCCTCATATGACATG+TGG 0.653216 8_4:-11096134 Msa1313250:three_prime_UTR
TATCCCTTCAGGCCAAAGAG+TGG 0.666606 8_4:-11097175 Msa1313250:CDS
TCTCAACTAATCTTGCTGCA+AGG 0.675422 8_4:-11096261 Msa1313250:CDS
CATCAAGGGATACAACCAGA+AGG 0.683587 8_4:-11097060 Msa1313250:CDS
ATCTCATCTGCTAAACGCTG+AGG 0.692989 8_4:+11097096 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATCAATTTCTTTAT+GGG + chr8_4:11096552-11096571 None:intergenic 10.0%
!! AAAATTAATCAATTTCTTTA+TGG + chr8_4:11096553-11096572 None:intergenic 10.0%
!!! AAAATAAAAACTTTTTTCTT+TGG - chr8_4:11097017-11097036 Msa1313250:CDS 10.0%
!! TTACTCAAAATAATTAAAAA+TGG + chr8_4:11099109-11099128 None:intergenic 10.0%
!!! TTTGTTTATTTGTTTTATTT+TGG - chr8_4:11099154-11099173 Msa1313250:CDS 10.0%
!!! TTGTTTATTTGTTTTATTTT+GGG - chr8_4:11099155-11099174 Msa1313250:CDS 10.0%
!!! AAAAAAAAAAAAGTACTCTA+AGG + chr8_4:11096286-11096305 None:intergenic 15.0%
!! TGATATAACAAACAAAATTT+AGG - chr8_4:11096504-11096523 Msa1313250:CDS 15.0%
!!! AACAAAATTTAGGATATTAT+CGG - chr8_4:11096514-11096533 Msa1313250:CDS 15.0%
!! AATTAATCAATTTCTTTATG+GGG + chr8_4:11096551-11096570 None:intergenic 15.0%
!!! ATGATTTTTCTAAAAAATGA+CGG + chr8_4:11096850-11096869 None:intergenic 15.0%
!! TTTAAAAAAATCTTTCTTGA+AGG - chr8_4:11096890-11096909 Msa1313250:CDS 15.0%
!!! TAAATGTTATTTAACCAAAT+AGG + chr8_4:11097052-11097071 None:intergenic 15.0%
!! ATTTGGTTAAATAACATTTA+AGG - chr8_4:11097052-11097071 Msa1313250:CDS 15.0%
!!! TAATTTGTTCAAAACATTTA+AGG - chr8_4:11097078-11097097 Msa1313250:CDS 15.0%
!!! TTATAACACATTTTAAGATA+TGG - chr8_4:11097495-11097514 Msa1313250:intron 15.0%
!! TTTAATTAGATTTGTACTTA+AGG - chr8_4:11097741-11097760 Msa1313250:intron 15.0%
!!! GACCAATTTTATTATTATTT+AGG + chr8_4:11097843-11097862 None:intergenic 15.0%
!! AATAATAATAAAATTGGTCA+TGG - chr8_4:11097844-11097863 Msa1313250:intron 15.0%
!! AATTGTGAGGTTAAAAATTT+AGG - chr8_4:11095922-11095941 Msa1313250:three_prime_UTR 20.0%
!! GTATTCTTCATAAATAAAAG+TGG + chr8_4:11096123-11096142 None:intergenic 20.0%
!!! TTTGTTCGACTTATATTTTT+AGG - chr8_4:11096225-11096244 Msa1313250:CDS 20.0%
!! CATGCTAATAAATATTCTAA+GGG + chr8_4:11096319-11096338 None:intergenic 20.0%
!! TCATGCTAATAAATATTCTA+AGG + chr8_4:11096320-11096339 None:intergenic 20.0%
!!! ATGTTTTTCTAACTATTACT+TGG + chr8_4:11096740-11096759 None:intergenic 20.0%
!! TAATAGTTAGAAAAACATTG+TGG - chr8_4:11096742-11096761 Msa1313250:CDS 20.0%
!! AGATACTATAAAGTCAATAA+AGG - chr8_4:11097248-11097267 Msa1313250:CDS 20.0%
!! TTTAAAAAAGTCCTATACAA+AGG - chr8_4:11097339-11097358 Msa1313250:CDS 20.0%
!! TATGGACAAAAAAATATTGT+AGG - chr8_4:11097467-11097486 Msa1313250:intron 20.0%
!! ATGGACAAAAAAATATTGTA+GGG - chr8_4:11097468-11097487 Msa1313250:intron 20.0%
!!! TATTTTTTAGAAATATGAGG+TGG + chr8_4:11097627-11097646 None:intergenic 20.0%
!!! TGGTATTTTTTAGAAATATG+AGG + chr8_4:11097630-11097649 None:intergenic 20.0%
!!! ATTTCTAAAAAATACCATAG+CGG - chr8_4:11097633-11097652 Msa1313250:intron 20.0%
!!! AAATAGCATTTTTGTTCAAA+AGG - chr8_4:11097686-11097705 Msa1313250:intron 20.0%
!! AAAAAAACATAGTTTCTTTG+TGG + chr8_4:11097723-11097742 None:intergenic 20.0%
!! GGCCTAAATAATAATAAAAT+TGG - chr8_4:11097838-11097857 Msa1313250:intron 20.0%
!! AGAATAAAAATGCTGAAATA+TGG - chr8_4:11098214-11098233 Msa1313250:intron 20.0%
!! TCTTCAATTGTTAAATCTTT+AGG - chr8_4:11098390-11098409 Msa1313250:intron 20.0%
!!! CAATCAATAGTTCATTTTTA+TGG - chr8_4:11098687-11098706 Msa1313250:intron 20.0%
!! TATTGAACTTAATAGAAAAG+AGG + chr8_4:11099217-11099236 None:intergenic 20.0%
!!! AGTTCAATATTTTACCATAA+TGG - chr8_4:11099228-11099247 Msa1313250:CDS 20.0%
! AACATCGCATTATTATGTAA+GGG - chr8_4:11096649-11096668 Msa1313250:CDS 25.0%
! AAAAATCTTTCTTGAAGGAA+AGG - chr8_4:11096895-11096914 Msa1313250:CDS 25.0%
!!! TTTGGAAACTTTTTCCTATT+TGG - chr8_4:11097035-11097054 Msa1313250:CDS 25.0%
! CATAATATGCATAACAACAT+GGG - chr8_4:11097161-11097180 Msa1313250:CDS 25.0%
! TTTATAGTATCTTCATGGTT+TGG + chr8_4:11097240-11097259 None:intergenic 25.0%
! TTGACTTTATAGTATCTTCA+TGG + chr8_4:11097245-11097264 None:intergenic 25.0%
! TGTACCTGAATCATATATTA+TGG - chr8_4:11097449-11097468 Msa1313250:intron 25.0%
! TTGTCCATAATATATGATTC+AGG + chr8_4:11097456-11097475 None:intergenic 25.0%
! TAAAACTCGACAATTATCTT+AGG - chr8_4:11097567-11097586 Msa1313250:intron 25.0%
! AATAATAAAATTGGTCATGG+TGG - chr8_4:11097847-11097866 Msa1313250:intron 25.0%
!! GTTAATCTAATTGTTTCTTC+AGG - chr8_4:11098177-11098196 Msa1313250:intron 25.0%
!!! AGTACTGATTGCTTTAATTT+TGG + chr8_4:11098349-11098368 None:intergenic 25.0%
! AACCATTTCAAAAATCTACT+AGG + chr8_4:11098450-11098469 None:intergenic 25.0%
! AACATTGATATTGAGATGAA+TGG - chr8_4:11098492-11098511 Msa1313250:intron 25.0%
! ATAGGATACTTTCTTTCTAA+AGG - chr8_4:11098636-11098655 Msa1313250:intron 25.0%
!! ATGAACTATTGATTGCAAAA+TGG + chr8_4:11098682-11098701 None:intergenic 25.0%
!!! ATGTTGCAAAAGATTTTTAG+TGG - chr8_4:11099066-11099085 Msa1313250:intron 25.0%
!! AAAACTTTGGTGTATTTGAT+TGG - chr8_4:11099189-11099208 Msa1313250:CDS 25.0%
TCAAATGCATTCAAATTGTG+AGG - chr8_4:11095909-11095928 Msa1313250:three_prime_UTR 30.0%
TGTCCTAATGATATTGTTCA+AGG - chr8_4:11096150-11096169 Msa1313250:three_prime_UTR 30.0%
GATCCTTGAACAATATCATT+AGG + chr8_4:11096156-11096175 None:intergenic 30.0%
!! GTTACTATGTAATTTTAGCG+AGG - chr8_4:11096361-11096380 Msa1313250:CDS 30.0%
CGAGGTTTAAATAATGAGAA+GGG - chr8_4:11096379-11096398 Msa1313250:CDS 30.0%
GAGGTTTAAATAATGAGAAG+GGG - chr8_4:11096380-11096399 Msa1313250:CDS 30.0%
ATTTCTTTATGGGGATTTAG+TGG + chr8_4:11096542-11096561 None:intergenic 30.0%
ATCAACTGAATTATGCTCAT+AGG + chr8_4:11096626-11096645 None:intergenic 30.0%
GAACATCGCATTATTATGTA+AGG - chr8_4:11096648-11096667 Msa1313250:CDS 30.0%
! GTTTTTGAACTTCACCTTTA+AGG + chr8_4:11096710-11096729 None:intergenic 30.0%
AGTAGATAATTCAGTTCAGT+TGG - chr8_4:11096782-11096801 Msa1313250:CDS 30.0%
ATAATTCAGTTCAGTTGGTT+GGG - chr8_4:11096787-11096806 Msa1313250:CDS 30.0%
CCATGTTGTTATGCATATTA+TGG + chr8_4:11097163-11097182 None:intergenic 30.0%
CCATAATATGCATAACAACA+TGG - chr8_4:11097160-11097179 Msa1313250:CDS 30.0%
TGTAATTGAGAATTTAGACG+TGG - chr8_4:11097292-11097311 Msa1313250:CDS 30.0%
!!! TTAGACGTGGTTTTATAGAA+AGG - chr8_4:11097305-11097324 Msa1313250:CDS 30.0%
TCGACAATTATCTTAGGTTA+TGG - chr8_4:11097573-11097592 Msa1313250:intron 30.0%
CGACAATTATCTTAGGTTAT+GGG - chr8_4:11097574-11097593 Msa1313250:intron 30.0%
GACAATTATCTTAGGTTATG+GGG - chr8_4:11097575-11097594 Msa1313250:intron 30.0%
! GATATTAAACTTTTCTCTGC+AGG - chr8_4:11097763-11097782 Msa1313250:intron 30.0%
TATTATTTAGGCCATCTTCA+GGG + chr8_4:11097831-11097850 None:intergenic 30.0%
TTATTATTTAGGCCATCTTC+AGG + chr8_4:11097832-11097851 None:intergenic 30.0%
CGATATCTTTGTATGGAAAA+GGG + chr8_4:11098137-11098156 None:intergenic 30.0%
TCGATATCTTTGTATGGAAA+AGG + chr8_4:11098138-11098157 None:intergenic 30.0%
TTTGAGTCGATATCTTTGTA+TGG + chr8_4:11098144-11098163 None:intergenic 30.0%
!! ATATCGACTCAAACGATATA+GGG - chr8_4:11098151-11098170 Msa1313250:intron 30.0%
! TTTAGTCTTCTTAAGTTTCG+AGG - chr8_4:11098246-11098265 Msa1313250:intron 30.0%
AATGACAAAGTTCGTCTTAA+AGG - chr8_4:11098276-11098295 Msa1313250:intron 30.0%
!! CTCCTAGTAGATTTTTGAAA+TGG - chr8_4:11098445-11098464 Msa1313250:intron 30.0%
GGTTACATTTCTAATACCAA+TGG - chr8_4:11098513-11098532 Msa1313250:intron 30.0%
ATGTGAACAACACCATAAAA+GGG + chr8_4:11098618-11098637 None:intergenic 30.0%
TATGTGAACAACACCATAAA+AGG + chr8_4:11098619-11098638 None:intergenic 30.0%
!!! TTTTATGGTGTTGTTCACAT+AGG - chr8_4:11098618-11098637 Msa1313250:intron 30.0%
AAATGTGATTGTAGTTGTTG+AGG - chr8_4:11098779-11098798 Msa1313250:intron 30.0%
TGCAACATTGCACAAATTTA+AGG + chr8_4:11099054-11099073 None:intergenic 30.0%
! ATATTTTACCATAATGGCCA+TGG - chr8_4:11099234-11099253 Msa1313250:CDS 30.0%
AAAGATCTGTTGCTAGGTTT+GGG - chr8_4:11096006-11096025 Msa1313250:three_prime_UTR 35.0%
TTTGGGGGAAGATATTAACA+GGG - chr8_4:11096023-11096042 Msa1313250:three_prime_UTR 35.0%
GCGAGGTTTAAATAATGAGA+AGG - chr8_4:11096378-11096397 Msa1313250:CDS 35.0%
AAAACAGAAGACATGAACGA+AGG + chr8_4:11096446-11096465 None:intergenic 35.0%
! TTCGTTCATGTCTTCTGTTT+TGG - chr8_4:11096445-11096464 Msa1313250:CDS 35.0%
GATAATTCAGTTCAGTTGGT+TGG - chr8_4:11096786-11096805 Msa1313250:CDS 35.0%
TAATTCAGTTCAGTTGGTTG+GGG - chr8_4:11096788-11096807 Msa1313250:CDS 35.0%
ACTAGATGTTGCCTTTGTAT+AGG + chr8_4:11097353-11097372 None:intergenic 35.0%
! AAAAGTACACATCGTTTCTG+CGG - chr8_4:11097661-11097680 Msa1313250:intron 35.0%
! AACTTTTCTCTGCAGGTTAT+AGG - chr8_4:11097770-11097789 Msa1313250:intron 35.0%
AGGTTATAGGCAAAATGTGA+AGG - chr8_4:11097783-11097802 Msa1313250:intron 35.0%
AATGTGAAGGACATTGTTGA+AGG - chr8_4:11097796-11097815 Msa1313250:intron 35.0%
AATAAAATTGGTCATGGTGG+TGG - chr8_4:11097850-11097869 Msa1313250:intron 35.0%
AAGACTCACAATAAGAGTAG+AGG + chr8_4:11097909-11097928 None:intergenic 35.0%
! ACATGACACTTGACTTGAAA+TGG + chr8_4:11097940-11097959 None:intergenic 35.0%
! TTTCAAGTCAAGTGTCATGT+TGG - chr8_4:11097940-11097959 Msa1313250:intron 35.0%
!! GATATCGACTCAAACGATAT+AGG - chr8_4:11098150-11098169 Msa1313250:intron 35.0%
! CTAATTGTTTCTTCAGGATC+TGG - chr8_4:11098183-11098202 Msa1313250:intron 35.0%
GAAACTTCAGCATATAGAGA+AGG + chr8_4:11098324-11098343 None:intergenic 35.0%
AGCAATCAGTACTCCTATTA+TGG - chr8_4:11098356-11098375 Msa1313250:intron 35.0%
! GAAACTGCATTGACCATAAT+AGG + chr8_4:11098372-11098391 None:intergenic 35.0%
CATTTCTAATACCAATGGAG+AGG - chr8_4:11098518-11098537 Msa1313250:intron 35.0%
! CATCATTTGCTTCCCTTTTA+TGG - chr8_4:11098603-11098622 Msa1313250:intron 35.0%
TCTAAAGGATGCTATCCAAT+TGG - chr8_4:11098651-11098670 Msa1313250:intron 35.0%
! TGCAAAATGGACTTTCCAAT+TGG + chr8_4:11098669-11098688 None:intergenic 35.0%
! TCAAGCTTCAAGTTCAAGAA+AGG - chr8_4:11098712-11098731 Msa1313250:intron 35.0%
TCACACTTGTATGATCTCTA+GGG - chr8_4:11098806-11098825 Msa1313250:intron 35.0%
ATTCTACCACATGTCATATG+AGG + chr8_4:11099016-11099035 None:intergenic 35.0%
GGCTGAAGATTTGAAAACTT+TGG - chr8_4:11099176-11099195 Msa1313250:CDS 35.0%
TATACAATCCATGGCCATTA+TGG + chr8_4:11099245-11099264 None:intergenic 35.0%
ATCTTTGACTGCACACTCAA+TGG + chr8_4:11095992-11096011 None:intergenic 40.0%
CAAAGATCTGTTGCTAGGTT+TGG - chr8_4:11096005-11096024 Msa1313250:three_prime_UTR 40.0%
AAGATCTGTTGCTAGGTTTG+GGG - chr8_4:11096007-11096026 Msa1313250:three_prime_UTR 40.0%
GTTTGGGGGAAGATATTAAC+AGG - chr8_4:11096022-11096041 Msa1313250:three_prime_UTR 40.0%
AGATCATAGTTACTCACCTC+TGG + chr8_4:11096100-11096119 None:intergenic 40.0%
AATAATGAGAAGGGGTGATG+AGG - chr8_4:11096388-11096407 Msa1313250:CDS 40.0%
GCATTATTATGTAAGGGTCG+TGG - chr8_4:11096655-11096674 Msa1313250:CDS 40.0%
!! TCACCTTTAAGGTGAACAAG+TGG + chr8_4:11096699-11096718 None:intergenic 40.0%
TAGAATTTGCCTCTCTGGTT+TGG - chr8_4:11097184-11097203 Msa1313250:CDS 40.0%
TTCAGTTCGATTCTCTCCTA+GGG - chr8_4:11097207-11097226 Msa1313250:CDS 40.0%
!!! TGTACTTTTGAGATCCGCTA+TGG + chr8_4:11097650-11097669 None:intergenic 40.0%
! CATGTTGGTTATATCCCTTC+AGG - chr8_4:11097955-11097974 Msa1313250:intron 40.0%
ATGTGTTCAGCTTTGCATCA+AGG - chr8_4:11098066-11098085 Msa1313250:intron 40.0%
TGTGTTCAGCTTTGCATCAA+GGG - chr8_4:11098067-11098086 Msa1313250:intron 40.0%
AGTATGATAGCAACTCCTTC+TGG + chr8_4:11098099-11098118 None:intergenic 40.0%
! CTTTAGGAGAAGATCCAACA+AGG - chr8_4:11098406-11098425 Msa1313250:intron 40.0%
TCTACAAGCTCTTTCCTTGT+TGG + chr8_4:11098423-11098442 None:intergenic 40.0%
CTAATACCAATGGAGAGGTA+AGG - chr8_4:11098523-11098542 Msa1313250:intron 40.0%
ATCAAACCTTACCTCTCCAT+TGG + chr8_4:11098532-11098551 None:intergenic 40.0%
! CTGAAGTGAACTTGCCTTTT+TGG - chr8_4:11098568-11098587 Msa1313250:intron 40.0%
TGCTCACATTGTGACCAAAA+AGG + chr8_4:11098585-11098604 None:intergenic 40.0%
TGCAGAAACCATTTCACCAT+TGG - chr8_4:11098749-11098768 Msa1313250:intron 40.0%
GAAACCATTTCACCATTGGT+AGG - chr8_4:11098753-11098772 Msa1313250:intron 40.0%
ATCACATTTCCACCTACCAA+TGG + chr8_4:11098768-11098787 None:intergenic 40.0%
GTCACACTTGTATGATCTCT+AGG - chr8_4:11098805-11098824 Msa1313250:intron 40.0%
CACACTTGTATGATCTCTAG+GGG - chr8_4:11098807-11098826 Msa1313250:intron 40.0%
TCTAGGGGAATTGAGAAGTT+TGG - chr8_4:11098822-11098841 Msa1313250:intron 40.0%
!!! ACCTCAACCATTGCTGTTTT+AGG - chr8_4:11098852-11098871 Msa1313250:intron 40.0%
TCCTAAAACAGCAATGGTTG+AGG + chr8_4:11098856-11098875 None:intergenic 40.0%
! GAAGTGTCCTAAAACAGCAA+TGG + chr8_4:11098862-11098881 None:intergenic 40.0%
TCTCAACTAATCTTGCTGCA+AGG - chr8_4:11098880-11098899 Msa1313250:intron 40.0%
TGGAATACTCTCCAAGAACT+TGG + chr8_4:11098907-11098926 None:intergenic 40.0%
CAAGTGCTACATCTCTTGAA+GGG - chr8_4:11098925-11098944 Msa1313250:intron 40.0%
AGTCTTCCTCATATGACATG+TGG - chr8_4:11099007-11099026 Msa1313250:intron 40.0%
GCAGTCAAAGATCTGTTGCT+AGG - chr8_4:11096000-11096019 Msa1313250:three_prime_UTR 45.0%
AGATCTGTTGCTAGGTTTGG+GGG - chr8_4:11096008-11096027 Msa1313250:three_prime_UTR 45.0%
GGGGAAGATATTAACAGGGA+AGG - chr8_4:11096027-11096046 Msa1313250:three_prime_UTR 45.0%
GACACCATTTCGTGTTGCAA+AGG - chr8_4:11096059-11096078 Msa1313250:three_prime_UTR 45.0%
AAGGCCTTTGCAACACGAAA+TGG + chr8_4:11096066-11096085 None:intergenic 45.0%
! GTTGCAAAGGCCTTTTCTGA+AGG - chr8_4:11096072-11096091 Msa1313250:three_prime_UTR 45.0%
! ACCTCTGGTTCCTTCAGAAA+AGG + chr8_4:11096085-11096104 None:intergenic 45.0%
! AAAGCATTTGGTGACAGGGA+CGG + chr8_4:11096420-11096439 None:intergenic 45.0%
!! GTGGAAAGCATTTGGTGACA+GGG + chr8_4:11096424-11096443 None:intergenic 45.0%
!! GAACGAAGGTGGAAAGCATT+TGG + chr8_4:11096432-11096451 None:intergenic 45.0%
ACAGAAGACATGAACGAAGG+TGG + chr8_4:11096443-11096462 None:intergenic 45.0%
!! CCTTTAAGGTGAACAAGTGG+AGG + chr8_4:11096696-11096715 None:intergenic 45.0%
CCTCCACTTGTTCACCTTAA+AGG - chr8_4:11096693-11096712 Msa1313250:CDS 45.0%
ATGGGTAGAATTTGCCTCTC+TGG - chr8_4:11097179-11097198 Msa1313250:CDS 45.0%
GTTCAGTTCGATTCTCTCCT+AGG - chr8_4:11097206-11097225 Msa1313250:CDS 45.0%
TCAGTTCGATTCTCTCCTAG+GGG - chr8_4:11097208-11097227 Msa1313250:CDS 45.0%
!! ATGGTTTGGTTCTATCCCCT+AGG + chr8_4:11097226-11097245 None:intergenic 45.0%
GGTGCATTATTCCCTGAAGA+TGG - chr8_4:11097817-11097836 Msa1313250:intron 45.0%
TGCTTAAGCCTACCACTCTT+TGG + chr8_4:11097981-11098000 None:intergenic 45.0%
ATCTCATCTGCTAAACGCTG+AGG + chr8_4:11098048-11098067 None:intergenic 45.0%
CATCAAGGGATACAACCAGA+AGG - chr8_4:11098081-11098100 Msa1313250:intron 45.0%
TCGACTCAAACGATATAGGG+TGG - chr8_4:11098154-11098173 Msa1313250:intron 45.0%
CGACTCAAACGATATAGGGT+GGG - chr8_4:11098155-11098174 Msa1313250:intron 45.0%
AAGGACCACTCGATCAATGT+TGG - chr8_4:11098295-11098314 Msa1313250:intron 45.0%
ACCATTTCACCATTGGTAGG+TGG - chr8_4:11098756-11098775 Msa1313250:intron 45.0%
TCCACCTACCAATGGTGAAA+TGG + chr8_4:11098760-11098779 None:intergenic 45.0%
CCTTCAAGAGATGTAGCACT+TGG + chr8_4:11098927-11098946 None:intergenic 45.0%
CCAAGTGCTACATCTCTTGA+AGG - chr8_4:11098924-11098943 Msa1313250:intron 45.0%
!! TTATAAAAAAAATTCTTATA+AGG + chr8_4:11097420-11097439 None:intergenic 5.0%
!! GCCTTTTCTGAAGGAACCAG+AGG - chr8_4:11096081-11096100 Msa1313250:three_prime_UTR 50.0%
! AAGCATTTGGTGACAGGGAC+GGG + chr8_4:11096419-11096438 None:intergenic 50.0%
!! GGTGGAAAGCATTTGGTGAC+AGG + chr8_4:11096425-11096444 None:intergenic 50.0%
CGAACTGAACCAAACCAGAG+AGG + chr8_4:11097196-11097215 None:intergenic 50.0%
!! ATTGGTCATGGTGGTGGAGT+AGG - chr8_4:11097856-11097875 Msa1313250:intron 50.0%
TATCCCTTCAGGCCAAAGAG+TGG - chr8_4:11097966-11097985 Msa1313250:intron 50.0%
CTACCACTCTTTGGCCTGAA+GGG + chr8_4:11097972-11097991 None:intergenic 50.0%
CTTAAGCAAGCTTCCTCGTG+TGG - chr8_4:11097993-11098012 Msa1313250:intron 50.0%
GGACACCAACATTGATCGAG+TGG + chr8_4:11098303-11098322 None:intergenic 50.0%
TGCTGCAAGGACCAAGTTCT+TGG - chr8_4:11098893-11098912 Msa1313250:intron 50.0%
! AGCATTTGGTGACAGGGACG+GGG + chr8_4:11096418-11096437 None:intergenic 55.0%
CCTACCACTCTTTGGCCTGA+AGG + chr8_4:11097973-11097992 None:intergenic 55.0%
CCTTCAGGCCAAAGAGTGGT+AGG - chr8_4:11097970-11097989 Msa1313250:intron 55.0%
AAGCAAGCTTCCTCGTGTGG+CGG - chr8_4:11097996-11098015 Msa1313250:intron 55.0%
! GCATTTGGTGACAGGGACGG+GGG + chr8_4:11096417-11096436 None:intergenic 60.0%
!! GGTCATGGTGGTGGAGTAGG+TGG - chr8_4:11097859-11097878 Msa1313250:intron 60.0%
GCAAATGCTTCCGCCACACG+AGG + chr8_4:11098009-11098028 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 11095895 11099268 11095895 ID=Msa1313250;Name=Msa1313250
chr8_4 mRNA 11095895 11099268 11095895 ID=Msa1313250-mRNA-1;Parent=Msa1313250;Name=Msa1313250-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0.5|1|0|0.5|2|315|447
chr8_4 exon 11095895 11097378 11095895 ID=Msa1313250-mRNA-1:exon:4158;Parent=Msa1313250-mRNA-1
chr8_4 exon 11099094 11099268 11099094 ID=Msa1313250-mRNA-1:exon:4157;Parent=Msa1313250-mRNA-1
chr8_4 CDS 11099094 11099268 11099094 ID=Msa1313250-mRNA-1:cds;Parent=Msa1313250-mRNA-1
chr8_4 CDS 11096210 11097378 11096210 ID=Msa1313250-mRNA-1:cds;Parent=Msa1313250-mRNA-1
chr8_4 three_prime_UTR 11095895 11096209 11095895 ID=Msa1313250-mRNA-1:three_prime_utr;Parent=Msa1313250-mRNA-1
Gene Sequence

>Msa1313250

ATGGGGTCGTTTGGTCAAATGCATTCAAATTGTGAGGTTAAAAATTTAGGAAGTAATGATGAAGTAAGTTTTAAGAAAGAGATCAATAGAAGTGCCATTGAGTGTGCAGTCAAAGATCTGTTGCTAGGTTTGGGGGAAGATATTAACAGGGAAGGCATTATAAAGACACCATTTCGTTATAGGCAAAATGTGAAGGACATTGTTGAAGGTGCATTATTCCCTGAAGATGGCCTAAATAATAATAAAATTGGTCATGGTGGTGGAGTAGGTGGACTTGTGATTGTGAGAGATATTGACCTCTACTCTTATTGTGAGTCTTGCTTGTTACCATTTCAAGTCAAGTGTCATGTTGGTTATATCCCTTCAGGCCAAAGAGTGGTAGGCTTAAGCAAGCTTCCTCGTGTGGCGGAAGCATTTGCAAAACGACTTCAAGAGCCTCAGCGTTTAGCAGATGAGATGTGTTCAGCTTTGCATCAAGGGATACAACCAGAAGGAGTTGCTATCATACTACAATGCACACATATCCCTTTTCCATACAAAGATATCGACTCAAACGATATAGGGTGGGTTAATCTAATTGTTTCTTCAGGATCTGGTGTTTATGAGAATAAAAATGCTGAAATATGGAGTGATTTCTTTAGTCTTCTTAAGTTTCGAGGTATTGATAATGACAAAGTTCGTCTTAAAGGACCACTCGATCAATGTTGGTGTCCTTCTCTATATGCTGAAGTTTCATCCAAAATTAAAGCAATCAGTACTCCTATTATGGTCAATGCAGTTTCTTCAATTGTTAAATCTTTAGGAGAAGATCCAACAAGGAAAGAGCTTGTAGAAACTCCTAGTAGATTTTTGAAATGGTTGATGAACTTTCAATGTGCTAGTAACATTGATATTGAGATGAATGGTTACATTTCTAATACCAATGGAGAGGTAAGGTTTGATGACAATGAAATATATTCTGAAGTGAACTTGCCTTTTTGGTCACAATGTGAGCATCATTTGCTTCCCTTTTATGGTGTTGTTCACATAGGATACTTTCTTTCTAAAGGATGCTATCCAATTGGAAAGTCCATTTTGCAATCAATAGTTCATTTTTATGGCTTCAAGCTTCAAGTTCAAGAAAGGCTCACAAAGCAGATTGCAGAAACCATTTCACCATTGGTAGGTGGAAATGTGATTGTAGTTGTTGAGGCAAGTCACACTTGTATGATCTCTAGGGGAATTGAGAAGTTTGGAAGCAATACCTCAACCATTGCTGTTTTAGGACACTTCTCAACTAATCTTGCTGCAAGGACCAAGTTCTTGGAGAGTATTCCAAGTGCTACATCTCTTGAAGGGCAATGA
Protein sequence

>Msa1313250

MGSFGQMHSNCEVKNLGSNDEVSFKKEINRSAIECAVKDLLLGLGEDINREGIIKTPFRYRQNVKDIVEGALFPEDGLNNNKIGHGGGVGGLVIVRDIDLYSYCESCLLPFQVKCHVGYIPSGQRVVGLSKLPRVAEAFAKRLQEPQRLADEMCSALHQGIQPEGVAIILQCTHIPFPYKDIDSNDIGWVNLIVSSGSGVYENKNAEIWSDFFSLLKFRGIDNDKVRLKGPLDQCWCPSLYAEVSSKIKAISTPIMVNAVSSIVKSLGEDPTRKELVETPSRFLKWLMNFQCASNIDIEMNGYISNTNGEVRFDDNEIYSEVNLPFWSQCEHHLLPFYGVVHIGYFLSKGCYPIGKSILQSIVHFYGFKLQVQERLTKQIAETISPLVGGNVIVVVEASHTCMISRGIEKFGSNTSTIAVLGHFSTNLAARTKFLESIPSATSLEGQ*