AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1232680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1232680 MtrunA17_Chr8g0341821 93.973 448 15 2 1 436 1 448 0.0 868
Msa1232680 MtrunA17_Chr8g0339291 68.308 467 125 6 1 446 1 465 0.0 620
Msa1232680 MtrunA17_Chr7g0235071 66.523 463 125 7 1 443 1 453 0.0 607
Msa1232680 MtrunA17_Chr7g0238471 71.605 81 22 1 61 141 34 113 5.39e-37 131
Msa1232680 MtrunA17_Chr7g0238481 57.303 89 33 2 182 270 14 97 4.63e-24 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1232680 AT3G07270.1 56.103 467 179 9 1 447 1 461 1.48e-173 494
Msa1232680 AT3G07270.2 57.753 445 164 8 21 447 23 461 2.32e-173 494

Find 95 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACATTGCACAAATTTA+AGG 0.111527 8_2:+9243200 None:intergenic
AGTACTGATTGCTTTAATTT+TGG 0.156856 8_2:+9243909 None:intergenic
TTTATAGATAGATTTATTTA+TGG 0.170581 8_2:+9243282 None:intergenic
TATATGAACAACACCATAAA+AGG 0.177080 8_2:+9243639 None:intergenic
TCTGCTTTGTGAGCCTTTCT+TGG 0.182440 8_2:+9243530 None:intergenic
AATGACAAAGTTCGTCTTAA+AGG 0.202003 8_2:-9243979 Msa1232680:CDS
TCTTCAATTGTTAAATCTTT+AGG 0.230854 8_2:-9243865 Msa1232680:CDS
GGCCTAAATAATAATAAAAT+TGG 0.265269 8_2:-9244417 Msa1232680:CDS
CAAAGATCTGTTACTAGGTT+TGG 0.272301 8_2:-9246539 Msa1232680:CDS
ATGAACTATTGATTGTAAAA+TGG 0.296775 8_2:+9243576 None:intergenic
AAAGATCTGTTACTAGGTTT+GGG 0.298638 8_2:-9246538 Msa1232680:CDS
GTTAAGTTACTTGTTTCTTC+AGG 0.301438 8_2:-9244078 Msa1232680:CDS
TTTAGTCTTCTAAAGTTTAG+AGG 0.313951 8_2:-9244009 Msa1232680:CDS
TTATTATTTAGGCCATCTTC+AGG 0.323009 8_2:+9244426 None:intergenic
TCTACAAGCTCTTTCCTTGT+TGG 0.323847 8_2:+9243835 None:intergenic
GAAACTGCATTGACCATAAT+AGG 0.333087 8_2:+9243886 None:intergenic
TGCTTAAGCCTACCACTCTT+TGG 0.341064 8_2:+9244277 None:intergenic
CATGTTGGTTATATCCCTTC+AGG 0.349179 8_2:-9244300 Msa1232680:CDS
GATATCGACTCAAACGATAT+AGG 0.353026 8_2:-9244105 Msa1232680:CDS
TTACTTGTTTCTTCAGGATC+TGG 0.355301 8_2:-9244072 Msa1232680:CDS
TGTAAAATGGACTTTCCAAT+TGG 0.369408 8_2:+9243589 None:intergenic
ACATGACACTTGACTTGAAA+TGG 0.378614 8_2:+9244318 None:intergenic
AGTATGATAGCAACTCCTTC+TGG 0.380673 8_2:+9244159 None:intergenic
TGCTCACATTGTGACCAAAA+AGG 0.383650 8_2:+9243673 None:intergenic
ATAGGATACTTTCTTTCTAA+AGG 0.384378 8_2:-9243619 Msa1232680:CDS
TCTAAAGGATGTTATCCAAT+TGG 0.386154 8_2:-9243604 Msa1232680:CDS
AGCAATCAGTACTCCTATTA+TGG 0.386350 8_2:-9243899 Msa1232680:CDS
TCGATATCTTTGTATGGAAA+AGG 0.388798 8_2:+9244120 None:intergenic
CCAAGTGCTACATCTCTTGA+AGG 0.424595 8_2:-9243331 Msa1232680:CDS
ATATCGACTCAAACGATATA+GGG 0.424779 8_2:-9244104 Msa1232680:CDS
TTTGAGTCGATATCTTTGTA+TGG 0.426394 8_2:+9244114 None:intergenic
TAAACTTTGGTGTATTTGAT+TGG 0.427298 8_2:-9243062 Msa1232680:three_prime_UTR
AATAAAATTGGTCATGGTGG+TGG 0.440425 8_2:-9244405 Msa1232680:CDS
TTTCAGTTAATGGGGTCGTT+TGG 0.445001 8_2:-9246658 None:intergenic
TCAAACTTCAAGTCCAAGAA+AGG 0.460674 8_2:-9243543 Msa1232680:CDS
GGCTGAAGATTTGTAAACTT+TGG 0.476216 8_2:-9243075 Msa1232680:three_prime_UTR
GTCACACTTGTATGATCTCT+AGG 0.476571 8_2:-9243450 Msa1232680:CDS
ATCTTTGACTGCACACTCAA+TGG 0.481435 8_2:+9246555 None:intergenic
TCCACCTACCAATGGTGAAA+GGG 0.484027 8_2:+9243498 None:intergenic
ATTGGTCATGGTGGTGGAGT+AGG 0.493372 8_2:-9244399 Msa1232680:CDS
TGCAGAAACCCTTTCACCAT+TGG 0.500531 8_2:-9243506 Msa1232680:CDS
TGTGTTCAGCTTTGCATCAA+GGG 0.508751 8_2:-9244188 Msa1232680:CDS
CGATATCTTTGTATGGAAAA+GGG 0.509754 8_2:+9244121 None:intergenic
TTCCACCTACCAATGGTGAA+AGG 0.509895 8_2:+9243497 None:intergenic
ATGTGTTCAGCTTTGCATCA+AGG 0.516850 8_2:-9244189 Msa1232680:CDS
CTAATACCAATGGAGAGGTA+AGG 0.520921 8_2:-9243732 Msa1232680:CDS
CTACCACTCTTTGGCCTGAA+GGG 0.523240 8_2:+9244286 None:intergenic
CCTACCACTCTTTGGCCTGA+AGG 0.523497 8_2:+9244285 None:intergenic
ATTCTACCACATGTCATATG+AGG 0.525458 8_2:+9243238 None:intergenic
CCTTCAAGAGATGTAGCACT+TGG 0.525506 8_2:+9243331 None:intergenic
TCCTAAAACAGCAATGGTTG+CGG 0.528343 8_2:+9243402 None:intergenic
TTACCACGTGATAATGGTCA+TGG 0.528850 8_2:-9243012 Msa1232680:three_prime_UTR
AACATTGATATAGAGATGAA+TGG 0.535613 8_2:-9243763 Msa1232680:CDS
TATTATTTAGGCCATCTTCA+GGG 0.537173 8_2:+9244427 None:intergenic
ATCAAACCTTACCTCTCCAT+TGG 0.540240 8_2:+9243726 None:intergenic
TCACACTTGTATGATCTCTA+GGG 0.541059 8_2:-9243449 Msa1232680:CDS
AATAATAATAAAATTGGTCA+TGG 0.542699 8_2:-9244411 Msa1232680:CDS
ATATGAACAACACCATAAAA+GGG 0.543872 8_2:+9243640 None:intergenic
AATAATAAAATTGGTCATGG+TGG 0.544701 8_2:-9244408 Msa1232680:CDS
GGTTACATTTCTAATACCAA+TGG 0.551456 8_2:-9243742 Msa1232680:CDS
TATTGAACTTAATAGAAAAG+AGG 0.553539 8_2:+9243037 None:intergenic
GCAGTCAAAGATCTGTTACT+AGG 0.561012 8_2:-9246544 Msa1232680:CDS
GGTGCATTATTCCCTGAAGA+TGG 0.565188 8_2:-9244438 Msa1232680:CDS
CTTAAGCAAGCTTCCTCGTG+TGG 0.570483 8_2:-9244262 Msa1232680:CDS
CCTTCAGGCCAAAGAGTGGT+AGG 0.576895 8_2:-9244285 Msa1232680:CDS
TTTCAAGTCAAGTGTCATGT+TGG 0.580286 8_2:-9244315 Msa1232680:CDS
GGGGAAGATATTAATAGGGA+AGG 0.581098 8_2:-9246517 Msa1232680:CDS
AAGATCTGTTACTAGGTTTG+GGG 0.583510 8_2:-9246537 Msa1232680:CDS
ACCCTTTCACCATTGGTAGG+TGG 0.594194 8_2:-9243499 Msa1232680:CDS
GGTCATGGTGGTGGAGTAGG+TGG 0.598165 8_2:-9244396 Msa1232680:CDS
GAAACCCTTTCACCATTGGT+AGG 0.598373 8_2:-9243502 Msa1232680:CDS
AAGGACCACTAGATCAATGT+TGG 0.603976 8_2:-9243960 Msa1232680:CDS
AATGTGAAGGACATTGTTGA+AGG 0.604917 8_2:-9244459 Msa1232680:CDS
GGACACCAACATTGATCTAG+TGG 0.609250 8_2:+9243955 None:intergenic
GAAACTTCAGCATATAGAGA+AGG 0.616046 8_2:+9243934 None:intergenic
AAGACTCACAATAAGAGTAG+AGG 0.617418 8_2:+9244349 None:intergenic
TCGACTCAAACGATATAGGG+TGG 0.618583 8_2:-9244101 Msa1232680:CDS
CGACTCAAACGATATAGGGT+GGG 0.620043 8_2:-9244100 Msa1232680:CDS
CAAGTGCTACATCTCTTGAA+GGG 0.625994 8_2:-9243330 Msa1232680:CDS
AAGCAAGCTTCCTCGTGTGG+CGG 0.628840 8_2:-9244259 Msa1232680:CDS
AGATCTGTTACTAGGTTTGG+GGG 0.630337 8_2:-9246536 Msa1232680:CDS
CTTTAGGAGAAGATCCAACA+AGG 0.631850 8_2:-9243849 Msa1232680:CDS
CACACTTGTATGATCTCTAG+GGG 0.636802 8_2:-9243448 Msa1232680:CDS
AGGTTATAGGCAAAATGTGA+AGG 0.638395 8_2:-9244472 Msa1232680:CDS
ATCACATTTCCACCTACCAA+TGG 0.639419 8_2:+9243490 None:intergenic
GCAAATGCTTCCGCCACACG+AGG 0.643408 8_2:+9244249 None:intergenic
GAAGTGTCCTAAAACAGCAA+TGG 0.645074 8_2:+9243396 None:intergenic
CATTTCTAATACCAATGGAG+AGG 0.648093 8_2:-9243737 Msa1232680:CDS
AGTCTTCCTCATATGACATG+TGG 0.653216 8_2:-9243244 Msa1232680:three_prime_UTR
TATCCCTTCAGGCCAAAGAG+TGG 0.666606 8_2:-9244289 Msa1232680:CDS
TCTCAACTAATCTTGCTGCA+AGG 0.675422 8_2:-9243375 Msa1232680:CDS
CATCAAGGGATACAACCAGA+AGG 0.683587 8_2:-9244174 Msa1232680:CDS
ATCTCATCTGCTAAACGCTG+AGG 0.692989 8_2:+9244210 None:intergenic
TCAAATGCATTCAAACTGTG+AGG 0.746780 8_2:-9246635 Msa1232680:CDS
AATCCATGACCATTATCACG+TGG 0.750173 8_2:+9243009 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATCAATTTCTTTAT+GGG + chr8_2:9243650-9243669 None:intergenic 10.0%
!! AAAATTAATCAATTTCTTTA+TGG + chr8_2:9243651-9243670 None:intergenic 10.0%
!!! ATTTTAAACTAATATAATAG+CGG - chr8_2:9243666-9243685 Msa1232680:CDS 10.0%
!!! AAAATAAAAACTTTTTTCTT+TGG - chr8_2:9244418-9244437 Msa1232680:CDS 10.0%
!! TCATAAAAAAAATTCTTATA+AGG + chr8_2:9244821-9244840 None:intergenic 10.0%
!! TTTATAGATAGATTTATTTA+TGG + chr8_2:9246370-9246389 None:intergenic 10.0%
!! TTACTCAAAATAATTAAAAA+TGG + chr8_2:9246507-9246526 None:intergenic 10.0%
!!! TTTGTTTATTTGTTTTATTT+TGG - chr8_2:9246552-9246571 Msa1232680:CDS 10.0%
!!! TTGTTTATTTGTTTTATTTT+GGG - chr8_2:9246553-9246572 Msa1232680:CDS 10.0%
!! TGATATAACAAACAAAATTT+AGG - chr8_2:9243602-9243621 Msa1232680:CDS 15.0%
!!! AACAAAATTTAGGATATTAT+TGG - chr8_2:9243612-9243631 Msa1232680:CDS 15.0%
!! AATTAATCAATTTCTTTATG+GGG + chr8_2:9243649-9243668 None:intergenic 15.0%
!!! TTTTAAACTAATATAATAGC+GGG - chr8_2:9243667-9243686 Msa1232680:CDS 15.0%
!! TTAAAAAAAATCTTTCTTGA+AGG - chr8_2:9244291-9244310 Msa1232680:CDS 15.0%
!!! TAAATGTTATTTAACCAAAT+AGG + chr8_2:9244453-9244472 None:intergenic 15.0%
!! ATTTGGTTAAATAACATTTA+AGG - chr8_2:9244453-9244472 Msa1232680:CDS 15.0%
!! TAATTTCTTCAAAACATTTA+AGG - chr8_2:9244479-9244498 Msa1232680:intron 15.0%
!!! TTATAACACATTTTAAGATA+TGG - chr8_2:9244896-9244915 Msa1232680:intron 15.0%
!! TTTAATTAGATTTGTACTTA+AGG - chr8_2:9245135-9245154 Msa1232680:intron 15.0%
!!! GACCAATTTTATTATTATTT+AGG + chr8_2:9245237-9245256 None:intergenic 15.0%
!! AATAATAATAAAATTGGTCA+TGG - chr8_2:9245238-9245257 Msa1232680:intron 15.0%
!!! TTCAAAAAAAAAGTACACTA+AGG + chr8_2:9243391-9243410 None:intergenic 20.0%
!! TTTAAACTAATATAATAGCG+GGG - chr8_2:9243668-9243687 Msa1232680:CDS 20.0%
!!! AATTGAGATTTGCTATTATA+GGG + chr8_2:9243965-9243984 None:intergenic 20.0%
!!! AAATTGAGATTTGCTATTAT+AGG + chr8_2:9243966-9243985 None:intergenic 20.0%
!! AACATCACATTATATATGTA+AGG - chr8_2:9244034-9244053 Msa1232680:CDS 20.0%
!! ACATCACATTATATATGTAA+GGG - chr8_2:9244035-9244054 Msa1232680:CDS 20.0%
!!! ATGTTTTTCTAACTATTACT+TGG + chr8_2:9244126-9244145 None:intergenic 20.0%
!! TAATAGTTAGAAAAACATTG+TGG - chr8_2:9244128-9244147 Msa1232680:CDS 20.0%
!!! GTGATTTTTCTAAAAAATGA+CGG + chr8_2:9244254-9244273 None:intergenic 20.0%
!! TTTAAAAAAGTCCTATACAA+AGG - chr8_2:9244740-9244759 Msa1232680:intron 20.0%
!! TATGGACAAAAAAATATTGT+AGG - chr8_2:9244868-9244887 Msa1232680:intron 20.0%
!! ATGGACAAAAAAATATTGTA+GGG - chr8_2:9244869-9244888 Msa1232680:intron 20.0%
!! ACAATTATCTTAGGTTATAT+GGG - chr8_2:9244967-9244986 Msa1232680:intron 20.0%
!!! TATTTTTTAGAAATATGAGG+TGG + chr8_2:9245021-9245040 None:intergenic 20.0%
!!! TGGTATTTTTTAGAAATATG+AGG + chr8_2:9245024-9245043 None:intergenic 20.0%
!!! ATTTCTAAAAAATACCATAG+CGG - chr8_2:9245027-9245046 Msa1232680:intron 20.0%
!!! AAATAGCATTTTTGTTCAAA+AGG - chr8_2:9245080-9245099 Msa1232680:intron 20.0%
!! AAAAAAACATAGTTTCTTTG+TGG + chr8_2:9245117-9245136 None:intergenic 20.0%
!! GGCCTAAATAATAATAAAAT+TGG - chr8_2:9245232-9245251 Msa1232680:intron 20.0%
!! TCTTCAATTGTTAAATCTTT+AGG - chr8_2:9245784-9245803 Msa1232680:intron 20.0%
!!! ATGAACTATTGATTGTAAAA+TGG + chr8_2:9246076-9246095 None:intergenic 20.0%
!!! CAATCAATAGTTCATTTTTA+TGG - chr8_2:9246081-9246100 Msa1232680:intron 20.0%
!! TATTGAACTTAATAGAAAAG+AGG + chr8_2:9246615-9246634 None:intergenic 20.0%
! GCATTCTTCATAAATAAAAG+TGG + chr8_2:9243228-9243247 None:intergenic 25.0%
! TGTCCTAATGATATTGTTTA+CGG - chr8_2:9243255-9243274 Msa1232680:three_prime_UTR 25.0%
!!! GTTACTATGTAATTTTAGTG+AGG - chr8_2:9243459-9243478 Msa1232680:CDS 25.0%
! TGAGGTTTGAATAATAAGAA+GGG - chr8_2:9243477-9243496 Msa1232680:CDS 25.0%
! TTGCAATTTATCTTCATTCA+GGG + chr8_2:9243741-9243760 None:intergenic 25.0%
! TTTGCAATTTATCTTCATTC+AGG + chr8_2:9243742-9243761 None:intergenic 25.0%
!! AGTTCATACAAACTATTTTC+TGG - chr8_2:9244185-9244204 Msa1232680:CDS 25.0%
!!! ATACAAACTATTTTCTGGTT+GGG - chr8_2:9244190-9244209 Msa1232680:CDS 25.0%
!!! TTTGGAAACTTTTTCCTATT+TGG - chr8_2:9244436-9244455 Msa1232680:CDS 25.0%
! CATAATATGCATAACAACAT+GGG - chr8_2:9244562-9244581 Msa1232680:intron 25.0%
! AGATACGATAAAGTCAATAA+AGG - chr8_2:9244649-9244668 Msa1232680:intron 25.0%
! TGTACCTGAATCATATATTA+TGG - chr8_2:9244850-9244869 Msa1232680:intron 25.0%
! TTGTCCATAATATATGATTC+AGG + chr8_2:9244857-9244876 None:intergenic 25.0%
! TAAAACTCGACAATTATCTT+AGG - chr8_2:9244958-9244977 Msa1232680:intron 25.0%
! GACAATTATCTTAGGTTATA+TGG - chr8_2:9244966-9244985 Msa1232680:intron 25.0%
! CAATTATCTTAGGTTATATG+GGG - chr8_2:9244968-9244987 Msa1232680:intron 25.0%
! AATAATAAAATTGGTCATGG+TGG - chr8_2:9245241-9245260 Msa1232680:intron 25.0%
! AGAACAAAAATGCTGAAATA+TGG - chr8_2:9245608-9245627 Msa1232680:intron 25.0%
!!! TTTAGTCTTCTAAAGTTTAG+AGG - chr8_2:9245640-9245659 Msa1232680:intron 25.0%
!!! AGTACTGATTGCTTTAATTT+TGG + chr8_2:9245743-9245762 None:intergenic 25.0%
! AACCATTTCAAAAATCTAGT+AGG + chr8_2:9245844-9245863 None:intergenic 25.0%
! AACATTGATATAGAGATGAA+TGG - chr8_2:9245886-9245905 Msa1232680:intron 25.0%
! ATATGAACAACACCATAAAA+GGG + chr8_2:9246012-9246031 None:intergenic 25.0%
! TATATGAACAACACCATAAA+AGG + chr8_2:9246013-9246032 None:intergenic 25.0%
!!! TTTTATGGTGTTGTTCATAT+AGG - chr8_2:9246012-9246031 Msa1232680:intron 25.0%
! ATAGGATACTTTCTTTCTAA+AGG - chr8_2:9246030-9246049 Msa1232680:intron 25.0%
!!! ATGTTGCAAAAGATTTTTAG+TGG - chr8_2:9246464-9246483 Msa1232680:intron 25.0%
!! TAAACTTTGGTGTATTTGAT+TGG - chr8_2:9246587-9246606 Msa1232680:CDS 25.0%
!! AATATTTTACCACGTGATAA+TGG - chr8_2:9246631-9246650 Msa1232680:CDS 25.0%
AAAGATCTGTTACTAGGTTT+GGG - chr8_2:9243111-9243130 Msa1232680:three_prime_UTR 30.0%
TTTGGGGGAAGATATTAATA+GGG - chr8_2:9243128-9243147 Msa1232680:three_prime_UTR 30.0%
GATCCGTAAACAATATCATT+AGG + chr8_2:9243261-9243280 None:intergenic 30.0%
! TGTGTTCGACTTATATTTTC+AGG - chr8_2:9243330-9243349 Msa1232680:CDS 30.0%
GTGAGGTTTGAATAATAAGA+AGG - chr8_2:9243476-9243495 Msa1232680:CDS 30.0%
GAGGTTTGAATAATAAGAAG+GGG - chr8_2:9243478-9243497 Msa1232680:CDS 30.0%
AAAACATAAGACATGAACGA+AGG + chr8_2:9243544-9243563 None:intergenic 30.0%
! TTCGTTCATGTCTTATGTTT+TGG - chr8_2:9243543-9243562 Msa1232680:CDS 30.0%
ACTAACAAAGTCTACATTCA+GGG + chr8_2:9243770-9243789 None:intergenic 30.0%
TTTGCATATTATCTGCATTC+AGG - chr8_2:9243890-9243909 Msa1232680:CDS 30.0%
TTGCATATTATCTGCATTCA+GGG - chr8_2:9243891-9243910 Msa1232680:CDS 30.0%
CACATTATATATGTAAGGGT+CGG - chr8_2:9244039-9244058 Msa1232680:CDS 30.0%
ACATTATATATGTAAGGGTC+GGG - chr8_2:9244040-9244059 Msa1232680:CDS 30.0%
!! TCACCTTTAAGGTTAATAAG+TGG + chr8_2:9244085-9244104 None:intergenic 30.0%
GTTTATGAACTTCACCTTTA+AGG + chr8_2:9244096-9244115 None:intergenic 30.0%
!! CATACAAACTATTTTCTGGT+TGG - chr8_2:9244189-9244208 Msa1232680:CDS 30.0%
!!! TACAAACTATTTTCTGGTTG+GGG - chr8_2:9244191-9244210 Msa1232680:CDS 30.0%
CCATGTTGTTATGCATATTA+TGG + chr8_2:9244564-9244583 None:intergenic 30.0%
CCATAATATGCATAACAACA+TGG - chr8_2:9244561-9244580 Msa1232680:intron 30.0%
TTTATCGTATCTTCATGGTT+TGG + chr8_2:9244641-9244660 None:intergenic 30.0%
TTGACTTTATCGTATCTTCA+TGG + chr8_2:9244646-9244665 None:intergenic 30.0%
TGTAATTGAGAATTTAGACG+TGG - chr8_2:9244693-9244712 Msa1232680:intron 30.0%
!!! TTAGACGTGGTTTTATAGAA+AGG - chr8_2:9244706-9244725 Msa1232680:intron 30.0%
! GATATTAAACTTTTCTCTGC+AGG - chr8_2:9245157-9245176 Msa1232680:intron 30.0%
TATTATTTAGGCCATCTTCA+GGG + chr8_2:9245225-9245244 None:intergenic 30.0%
TTATTATTTAGGCCATCTTC+AGG + chr8_2:9245226-9245245 None:intergenic 30.0%
CGATATCTTTGTATGGAAAA+GGG + chr8_2:9245531-9245550 None:intergenic 30.0%
TCGATATCTTTGTATGGAAA+AGG + chr8_2:9245532-9245551 None:intergenic 30.0%
TTTGAGTCGATATCTTTGTA+TGG + chr8_2:9245538-9245557 None:intergenic 30.0%
!! ATATCGACTCAAACGATATA+GGG - chr8_2:9245545-9245564 Msa1232680:intron 30.0%
! GTTAAGTTACTTGTTTCTTC+AGG - chr8_2:9245571-9245590 Msa1232680:intron 30.0%
AATGACAAAGTTCGTCTTAA+AGG - chr8_2:9245670-9245689 Msa1232680:intron 30.0%
!! CTCCTACTAGATTTTTGAAA+TGG - chr8_2:9245839-9245858 Msa1232680:intron 30.0%
GGTTACATTTCTAATACCAA+TGG - chr8_2:9245907-9245926 Msa1232680:intron 30.0%
TCTAAAGGATGTTATCCAAT+TGG - chr8_2:9246045-9246064 Msa1232680:intron 30.0%
! TGTAAAATGGACTTTCCAAT+TGG + chr8_2:9246063-9246082 None:intergenic 30.0%
TCTAGGGGAATTGAAAAATT+TGG - chr8_2:9246216-9246235 Msa1232680:intron 30.0%
TGCAACATTGCACAAATTTA+AGG + chr8_2:9246452-9246471 None:intergenic 30.0%
TCAAATGCATTCAAACTGTG+AGG - chr8_2:9243014-9243033 Msa1232680:three_prime_UTR 35.0%
CAAAGATCTGTTACTAGGTT+TGG - chr8_2:9243110-9243129 Msa1232680:three_prime_UTR 35.0%
AAGATCTGTTACTAGGTTTG+GGG - chr8_2:9243112-9243131 Msa1232680:three_prime_UTR 35.0%
GTTTGGGGGAAGATATTAAT+AGG - chr8_2:9243127-9243146 Msa1232680:three_prime_UTR 35.0%
ATTTCTTTATGGGGATCTAG+TGG + chr8_2:9243640-9243659 None:intergenic 35.0%
! TAGTCACTTTAGTTCCTGAA+TGG - chr8_2:9243694-9243713 Msa1232680:CDS 35.0%
! AGTCACTTTAGTTCCTGAAT+GGG - chr8_2:9243695-9243714 Msa1232680:CDS 35.0%
GACTAACAAAGTCTACATTC+AGG + chr8_2:9243771-9243790 None:intergenic 35.0%
TCAGGGACTAAATTGACTAA+CGG + chr8_2:9243785-9243804 None:intergenic 35.0%
ACTAACGAAGTCTACATTCA+GGG + chr8_2:9243834-9243853 None:intergenic 35.0%
ATCAACTGAATTATGCTCAC+AGG + chr8_2:9244011-9244030 None:intergenic 35.0%
CATTATATATGTAAGGGTCG+GGG - chr8_2:9244041-9244060 Msa1232680:CDS 35.0%
!! CCTTTAAGGTTAATAAGTGG+AGG + chr8_2:9244082-9244101 None:intergenic 35.0%
CCTCCACTTATTAACCTTAA+AGG - chr8_2:9244079-9244098 Msa1232680:CDS 35.0%
!!! ACAAACTATTTTCTGGTTGG+GGG - chr8_2:9244192-9244211 Msa1232680:CDS 35.0%
TAGAATTTGCCTCTCTAGTT+TGG - chr8_2:9244585-9244604 Msa1232680:intron 35.0%
ACTAGATGTTGCCTTTGTAT+AGG + chr8_2:9244754-9244773 None:intergenic 35.0%
! AAAAGTACACATCGTTTCTG+CGG - chr8_2:9245055-9245074 Msa1232680:intron 35.0%
! AACTTTTCTCTGCAGGTTAT+AGG - chr8_2:9245164-9245183 Msa1232680:intron 35.0%
AGGTTATAGGCAAAATGTGA+AGG - chr8_2:9245177-9245196 Msa1232680:intron 35.0%
AATGTGAAGGACATTGTTGA+AGG - chr8_2:9245190-9245209 Msa1232680:intron 35.0%
AATAAAATTGGTCATGGTGG+TGG - chr8_2:9245244-9245263 Msa1232680:intron 35.0%
AAGACTCACAATAAGAGTAG+AGG + chr8_2:9245303-9245322 None:intergenic 35.0%
! ACATGACACTTGACTTGAAA+TGG + chr8_2:9245334-9245353 None:intergenic 35.0%
! TTTCAAGTCAAGTGTCATGT+TGG - chr8_2:9245334-9245353 Msa1232680:intron 35.0%
!! GATATCGACTCAAACGATAT+AGG - chr8_2:9245544-9245563 Msa1232680:intron 35.0%
! TTACTTGTTTCTTCAGGATC+TGG - chr8_2:9245577-9245596 Msa1232680:intron 35.0%
GAAACTTCAGCATATAGAGA+AGG + chr8_2:9245718-9245737 None:intergenic 35.0%
AGCAATCAGTACTCCTATTA+TGG - chr8_2:9245750-9245769 Msa1232680:intron 35.0%
! GAAACTGCATTGACCATAAT+AGG + chr8_2:9245766-9245785 None:intergenic 35.0%
CATTTCTAATACCAATGGAG+AGG - chr8_2:9245912-9245931 Msa1232680:intron 35.0%
! CATCATTTGCTTCCCTTTTA+TGG - chr8_2:9245997-9246016 Msa1232680:intron 35.0%
TCAAACTTCAAGTCCAAGAA+AGG - chr8_2:9246106-9246125 Msa1232680:intron 35.0%
TCACACTTGTATGATCTCTA+GGG - chr8_2:9246200-9246219 Msa1232680:intron 35.0%
TGGAATACTCTCCAAAAACT+TGG + chr8_2:9246301-9246320 None:intergenic 35.0%
ATTCTACCACATGTCATATG+AGG + chr8_2:9246414-9246433 None:intergenic 35.0%
GGCTGAAGATTTGTAAACTT+TGG - chr8_2:9246574-9246593 Msa1232680:CDS 35.0%
ATCTTTGACTGCACACTCAA+TGG + chr8_2:9243097-9243116 None:intergenic 40.0%
GCAGTCAAAGATCTGTTACT+AGG - chr8_2:9243105-9243124 Msa1232680:three_prime_UTR 40.0%
AGATCTGTTACTAGGTTTGG+GGG - chr8_2:9243113-9243132 Msa1232680:three_prime_UTR 40.0%
GGGGAAGATATTAATAGGGA+AGG - chr8_2:9243132-9243151 Msa1232680:three_prime_UTR 40.0%
AGATCATAGTTACTCACCTC+TGG + chr8_2:9243205-9243224 None:intergenic 40.0%
ACATAAGACATGAACGAAGG+TGG + chr8_2:9243541-9243560 None:intergenic 40.0%
! GTCACTTTAGTTCCTGAATG+GGG - chr8_2:9243696-9243715 Msa1232680:CDS 40.0%
GACTAACGAAGTCTACATTC+AGG + chr8_2:9243835-9243854 None:intergenic 40.0%
! AGTCACTTTAGTCCCTGAAT+GGG + chr8_2:9243937-9243956 None:intergenic 40.0%
! TAGTCACTTTAGTCCCTGAA+TGG + chr8_2:9243850-9243869 None:intergenic 40.0%
TTCAGTTCGATTCTCTCCTA+GGG - chr8_2:9244608-9244627 Msa1232680:intron 40.0%
!!! TGTACTTTTGAGATCCGCTA+TGG + chr8_2:9245044-9245063 None:intergenic 40.0%
! CATGTTGGTTATATCCCTTC+AGG - chr8_2:9245349-9245368 Msa1232680:intron 40.0%
ATGTGTTCAGCTTTGCATCA+AGG - chr8_2:9245460-9245479 Msa1232680:intron 40.0%
TGTGTTCAGCTTTGCATCAA+GGG - chr8_2:9245461-9245480 Msa1232680:intron 40.0%
AGTATGATAGCAACTCCTTC+TGG + chr8_2:9245493-9245512 None:intergenic 40.0%
AAGGACCACTAGATCAATGT+TGG - chr8_2:9245689-9245708 Msa1232680:intron 40.0%
! CTTTAGGAGAAGATCCAACA+AGG - chr8_2:9245800-9245819 Msa1232680:intron 40.0%
TCTACAAGCTCTTTCCTTGT+TGG + chr8_2:9245817-9245836 None:intergenic 40.0%
CTAATACCAATGGAGAGGTA+AGG - chr8_2:9245917-9245936 Msa1232680:intron 40.0%
ATCAAACCTTACCTCTCCAT+TGG + chr8_2:9245926-9245945 None:intergenic 40.0%
! CTGAAGTGAACTTGCCTTTT+TGG - chr8_2:9245962-9245981 Msa1232680:intron 40.0%
TGCTCACATTGTGACCAAAA+AGG + chr8_2:9245979-9245998 None:intergenic 40.0%
ATCACATTTCCACCTACCAA+TGG + chr8_2:9246162-9246181 None:intergenic 40.0%
GTCACACTTGTATGATCTCT+AGG - chr8_2:9246199-9246218 Msa1232680:intron 40.0%
CACACTTGTATGATCTCTAG+GGG - chr8_2:9246201-9246220 Msa1232680:intron 40.0%
TCCTAAAACAGCAATGGTTG+CGG + chr8_2:9246250-9246269 None:intergenic 40.0%
! GAAGTGTCCTAAAACAGCAA+TGG + chr8_2:9246256-9246275 None:intergenic 40.0%
TCTCAACTAATCTTGCTGCA+AGG - chr8_2:9246274-9246293 Msa1232680:intron 40.0%
CAAGTGCTACATCTCTTGAA+GGG - chr8_2:9246319-9246338 Msa1232680:intron 40.0%
AGTCTTCCTCATATGACATG+TGG - chr8_2:9246405-9246424 Msa1232680:intron 40.0%
!! TTACCACGTGATAATGGTCA+TGG - chr8_2:9246637-9246656 Msa1232680:CDS 40.0%
AATCCATGACCATTATCACG+TGG + chr8_2:9246643-9246662 None:intergenic 40.0%
GACACCATTTCGTGTTGCAA+AGG - chr8_2:9243164-9243183 Msa1232680:three_prime_UTR 45.0%
AAGGCCTTTGCAACACGAAA+TGG + chr8_2:9243171-9243190 None:intergenic 45.0%
! GTTGCAAAGGCCTTTTCTGA+AGG - chr8_2:9243177-9243196 Msa1232680:three_prime_UTR 45.0%
! ACCTCTGGTTCCTTCAGAAA+AGG + chr8_2:9243190-9243209 None:intergenic 45.0%
ACTAACGGAGTCTGCATTCA+GGG + chr8_2:9243802-9243821 None:intergenic 45.0%
TCCCTGAAATTGACCATGCA+GGG - chr8_2:9243862-9243881 Msa1232680:CDS 45.0%
TCCCTGCATGGTCAATTTCA+GGG + chr8_2:9243866-9243885 None:intergenic 45.0%
TATGCAAAACAGTCCCTGCA+TGG + chr8_2:9243878-9243897 None:intergenic 45.0%
! GTCACTTTAGTCCCTGAATG+GGG + chr8_2:9243936-9243955 None:intergenic 45.0%
CGAACTGAACCAAACTAGAG+AGG + chr8_2:9244597-9244616 None:intergenic 45.0%
GTTCAGTTCGATTCTCTCCT+AGG - chr8_2:9244607-9244626 Msa1232680:intron 45.0%
TCAGTTCGATTCTCTCCTAG+GGG - chr8_2:9244609-9244628 Msa1232680:intron 45.0%
!! ATGGTTTGGTTCTATCCCCT+AGG + chr8_2:9244627-9244646 None:intergenic 45.0%
GGTGCATTATTCCCTGAAGA+TGG - chr8_2:9245211-9245230 Msa1232680:intron 45.0%
TGCTTAAGCCTACCACTCTT+TGG + chr8_2:9245375-9245394 None:intergenic 45.0%
ATCTCATCTGCTAAACGCTG+AGG + chr8_2:9245442-9245461 None:intergenic 45.0%
CATCAAGGGATACAACCAGA+AGG - chr8_2:9245475-9245494 Msa1232680:intron 45.0%
TCGACTCAAACGATATAGGG+TGG - chr8_2:9245548-9245567 Msa1232680:intron 45.0%
CGACTCAAACGATATAGGGT+GGG - chr8_2:9245549-9245568 Msa1232680:intron 45.0%
GGACACCAACATTGATCTAG+TGG + chr8_2:9245697-9245716 None:intergenic 45.0%
TCTGCTTTGTGAGCCTTTCT+TGG + chr8_2:9246122-9246141 None:intergenic 45.0%
TGCAGAAACCCTTTCACCAT+TGG - chr8_2:9246143-9246162 Msa1232680:intron 45.0%
GAAACCCTTTCACCATTGGT+AGG - chr8_2:9246147-9246166 Msa1232680:intron 45.0%
TCCACCTACCAATGGTGAAA+GGG + chr8_2:9246154-9246173 None:intergenic 45.0%
TTCCACCTACCAATGGTGAA+AGG + chr8_2:9246155-9246174 None:intergenic 45.0%
!!! ACCGCAACCATTGCTGTTTT+AGG - chr8_2:9246246-9246265 Msa1232680:intron 45.0%
! TGCTGCAAGGACCAAGTTTT+TGG - chr8_2:9246287-9246306 Msa1232680:intron 45.0%
CCTTCAAGAGATGTAGCACT+TGG + chr8_2:9246321-9246340 None:intergenic 45.0%
CCAAGTGCTACATCTCTTGA+AGG - chr8_2:9246318-9246337 Msa1232680:intron 45.0%
!! GCCTTTTCTGAAGGAACCAG+AGG - chr8_2:9243186-9243205 Msa1232680:three_prime_UTR 50.0%
! AAAGCATCTGGTGACAGGGA+CGG + chr8_2:9243518-9243537 None:intergenic 50.0%
!! GTGGAAAGCATCTGGTGACA+GGG + chr8_2:9243522-9243541 None:intergenic 50.0%
!! GAACGAAGGTGGAAAGCATC+TGG + chr8_2:9243530-9243549 None:intergenic 50.0%
TGACGAATCGTTCCCCATTC+AGG + chr8_2:9243921-9243940 None:intergenic 50.0%
GACTAACGGAGTCTGCATTC+AGG + chr8_2:9243803-9243822 None:intergenic 50.0%
GTCCCTGAAATTGACCATGC+AGG - chr8_2:9243861-9243880 Msa1232680:CDS 50.0%
GTCCCTGCATGGTCAATTTC+AGG + chr8_2:9243867-9243886 None:intergenic 50.0%
TGACGAATCGTTCCCCATTC+AGG - chr8_2:9243921-9243940 Msa1232680:CDS 50.0%
GACGAATCGTTCCCCATTCA+GGG - chr8_2:9243922-9243941 Msa1232680:CDS 50.0%
!! ATTGGTCATGGTGGTGGAGT+AGG - chr8_2:9245250-9245269 Msa1232680:intron 50.0%
TATCCCTTCAGGCCAAAGAG+TGG - chr8_2:9245360-9245379 Msa1232680:intron 50.0%
CTACCACTCTTTGGCCTGAA+GGG + chr8_2:9245366-9245385 None:intergenic 50.0%
CTTAAGCAAGCTTCCTCGTG+TGG - chr8_2:9245387-9245406 Msa1232680:intron 50.0%
ACCCTTTCACCATTGGTAGG+TGG - chr8_2:9246150-9246169 Msa1232680:intron 50.0%
AAGCATCTGGTGACAGGGAC+GGG + chr8_2:9243517-9243536 None:intergenic 55.0%
!! GGTGGAAAGCATCTGGTGAC+AGG + chr8_2:9243523-9243542 None:intergenic 55.0%
CCTACCACTCTTTGGCCTGA+AGG + chr8_2:9245367-9245386 None:intergenic 55.0%
CCTTCAGGCCAAAGAGTGGT+AGG - chr8_2:9245364-9245383 Msa1232680:intron 55.0%
AAGCAAGCTTCCTCGTGTGG+CGG - chr8_2:9245390-9245409 Msa1232680:intron 55.0%
AGCATCTGGTGACAGGGACG+GGG + chr8_2:9243516-9243535 None:intergenic 60.0%
!! GGTCATGGTGGTGGAGTAGG+TGG - chr8_2:9245253-9245272 Msa1232680:intron 60.0%
GCAAATGCTTCCGCCACACG+AGG + chr8_2:9245403-9245422 None:intergenic 60.0%
GCATCTGGTGACAGGGACGG+GGG + chr8_2:9243515-9243534 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 9243000 9246671 9243000 ID=Msa1232680;Name=Msa1232680
chr8_2 mRNA 9243000 9246671 9243000 ID=Msa1232680-mRNA-1;Parent=Msa1232680;Name=Msa1232680-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0.5|1|0|0.5|2|324|447
chr8_2 exon 9243000 9244492 9243000 ID=Msa1232680-mRNA-1:exon:3616;Parent=Msa1232680-mRNA-1
chr8_2 exon 9246497 9246671 9246497 ID=Msa1232680-mRNA-1:exon:3615;Parent=Msa1232680-mRNA-1
chr8_2 CDS 9246497 9246671 9246497 ID=Msa1232680-mRNA-1:cds;Parent=Msa1232680-mRNA-1
chr8_2 CDS 9243324 9244492 9243324 ID=Msa1232680-mRNA-1:cds;Parent=Msa1232680-mRNA-1
chr8_2 three_prime_UTR 9243000 9243323 9243000 ID=Msa1232680-mRNA-1:three_prime_utr;Parent=Msa1232680-mRNA-1
Gene Sequence

>Msa1232680

ATGGGGTCGTTTGGTCAAATGCATTCAAACTGTGAGGTTGAAAATTTAGTAAGTAATGATGAAGTGAGTTTTAAGAAAGAGATCAATAGAAGTGCCATTGAGTGTGCAGTCAAAGATCTGTTACTAGGTTTGGGGGAAGATATTAATAGGGAAGGCATTATAAAGACACCATTTCGTTATAGGCAAAATGTGAAGGACATTGTTGAAGGTGCATTATTCCCTGAAGATGGCCTAAATAATAATAAAATTGGTCATGGTGGTGGAGTAGGTGGACTTGTGATTGTGAGAGATATTGACCTCTACTCTTATTGTGAGTCTTGCTTGTTACCATTTCAAGTCAAGTGTCATGTTGGTTATATCCCTTCAGGCCAAAGAGTGGTAGGCTTAAGCAAGCTTCCTCGTGTGGCGGAAGCATTTGCAAAACGACTTCAAGAGCCTCAGCGTTTAGCAGATGAGATGTGTTCAGCTTTGCATCAAGGGATACAACCAGAAGGAGTTGCTATCATACTACAATGCACACATATCCCTTTTCCATACAAAGATATCGACTCAAACGATATAGGGTGGGTTAAGTTACTTGTTTCTTCAGGATCTGGTGTTTATGAGAACAAAAATGCTGAAATATGGAGTGATTTCTTTAGTCTTCTAAAGTTTAGAGGTCTTGATAATGACAAAGTTCGTCTTAAAGGACCACTAGATCAATGTTGGTGTCCTTCTCTATATGCTGAAGTTTCATCCAAAATTAAAGCAATCAGTACTCCTATTATGGTCAATGCAGTTTCTTCAATTGTTAAATCTTTAGGAGAAGATCCAACAAGGAAAGAGCTTGTAGAAACTCCTACTAGATTTTTGAAATGGTTGATGAACTTTCAATGTGCTAGTAACATTGATATAGAGATGAATGGTTACATTTCTAATACCAATGGAGAGGTAAGGTTTGATGACAATGAAATATATTCTGAAGTGAACTTGCCTTTTTGGTCACAATGTGAGCATCATTTGCTTCCCTTTTATGGTGTTGTTCATATAGGATACTTTCTTTCTAAAGGATGTTATCCAATTGGAAAGTCCATTTTACAATCAATAGTTCATTTTTATGGTTTCAAACTTCAAGTCCAAGAAAGGCTCACAAAGCAGATTGCAGAAACCCTTTCACCATTGGTAGGTGGAAATGTGATTGTAGTTGTTGAAGCAAGTCACACTTGTATGATCTCTAGGGGAATTGAAAAATTTGGAAGCAATACCGCAACCATTGCTGTTTTAGGACACTTCTCAACTAATCTTGCTGCAAGGACCAAGTTTTTGGAGAGTATTCCAAGTGCTACATCTCTTGAAGGGCAATGA
Protein sequence

>Msa1232680

MGSFGQMHSNCEVENLVSNDEVSFKKEINRSAIECAVKDLLLGLGEDINREGIIKTPFRYRQNVKDIVEGALFPEDGLNNNKIGHGGGVGGLVIVRDIDLYSYCESCLLPFQVKCHVGYIPSGQRVVGLSKLPRVAEAFAKRLQEPQRLADEMCSALHQGIQPEGVAIILQCTHIPFPYKDIDSNDIGWVKLLVSSGSGVYENKNAEIWSDFFSLLKFRGLDNDKVRLKGPLDQCWCPSLYAEVSSKIKAISTPIMVNAVSSIVKSLGEDPTRKELVETPTRFLKWLMNFQCASNIDIEMNGYISNTNGEVRFDDNEIYSEVNLPFWSQCEHHLLPFYGVVHIGYFLSKGCYPIGKSILQSIVHFYGFKLQVQERLTKQIAETLSPLVGGNVIVVVEASHTCMISRGIEKFGSNTATIAVLGHFSTNLAARTKFLESIPSATSLEGQ*