AlfalfaGEDB | Msa1321590

M. sativa cultivar zhongmu-4 / Msa1321590

KEGG: K14431
GO: GO:0001101 GO:0002376 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009627 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045087 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141
PFAM: PF07716.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321590	XP_003627926.1	98.283	466	7	1	1	465	1	466	0.0	938

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321590	sp\|Q6IVC2\|TGAL1_ORYSJ	67.401	454	137	6	18	465	24	472	0.0	563

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321590	G7LGW2	98.283	466	7	1	1	465	1	466	0.0	938

TFs/TRs:

Gene ID	Type	Classification
Msa1321590	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0610550	Msa1321590	0.826487	2.640604e-54	-8.615850e-47
Msa0746170	Msa1321590	0.801299	9.612740e-49	-8.615850e-47
Msa0746210	Msa1321590	0.809536	1.802592e-50	-8.615850e-47
Msa0828590	Msa1321590	0.817204	3.723328e-52	-8.615850e-47
Msa0462600	Msa1321590	0.809658	1.696886e-50	-8.615850e-47
Msa0985180	Msa1321590	0.815003	1.154643e-51	-8.615850e-47
Msa1186760	Msa1321590	0.858181	9.823137e-63	-8.615850e-47
Msa0542560	Msa1321590	0.804995	1.653112e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321590	MtrunA17_Chr8g0353501	98.283	466	7	1	1	465	1	466	0.0	938
Msa1321590	MtrunA17_Chr7g0256211	67.377	469	132	9	1	465	1	452	0.0	588
Msa1321590	MtrunA17_Chr5g0422771	66.667	303	98	3	165	465	163	464	1.91e-142	416
Msa1321590	MtrunA17_Chr1g0210151	60.519	347	115	6	134	465	1	340	2.36e-136	396
Msa1321590	MtrunA17_Chr1g0174701	60.261	307	109	4	167	464	203	505	2.88e-129	384
Msa1321590	MtrunA17_Chr7g0258321	52.850	386	159	9	97	464	82	462	6.09e-129	381
Msa1321590	MtrunA17_Chr2g0321431	63.194	288	96	3	178	465	192	469	3.19e-124	370
Msa1321590	MtrunA17_Chr3g0124601	56.575	327	124	4	149	465	43	361	4.46e-119	352
Msa1321590	MtrunA17_Chr4g0026401	58.419	291	102	3	178	465	187	461	2.76e-113	342
Msa1321590	MtrunA17_Chr3g0115371	50.658	304	137	3	168	465	53	349	1.04e-94	290
Msa1321590	MtrunA17_Chr8g0382861	51.689	296	129	4	175	465	70	356	1.86e-94	289
Msa1321590	MtrunA17_Chr5g0424861	52.431	288	125	4	180	462	67	347	8.70e-90	277
Msa1321590	MtrunA17_Chr3g0093451	58.824	187	64	4	159	332	23	209	2.49e-73	230
Msa1321590	MtrunA17_Chr5g0424851	48.636	220	105	3	244	462	10	222	2.21e-59	201
Msa1321590	MtrunA17_Chr5g0424851	46.117	206	104	2	248	453	228	426	6.16e-55	189
Msa1321590	MtrunA17_Chr1g0196951	33.333	207	108	5	257	458	59	240	5.21e-23	97.4
Msa1321590	MtrunA17_Chr8g0384821	28.333	240	144	4	241	452	8	247	4.73e-18	84.0
Msa1321590	MtrunA17_Chr7g0242651	24.171	211	157	2	248	455	9	219	9.97e-13	67.8
Msa1321590	MtrunA17_Chr2g0315631	22.581	217	127	3	248	444	4	199	5.21e-12	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321590	AT3G12250.6	80.723	332	62	2	134	465	1	330	0.0	528
Msa1321590	AT3G12250.2	80.723	332	62	2	134	465	1	330	0.0	528
Msa1321590	AT3G12250.7	80.723	332	62	2	134	465	1	330	0.0	528
Msa1321590	AT3G12250.1	80.723	332	62	2	134	465	1	330	0.0	528
Msa1321590	AT3G12250.4	75.350	357	61	3	134	465	1	355	0.0	515
Msa1321590	AT3G12250.5	82.566	304	52	1	162	465	1	303	5.40e-178	500
Msa1321590	AT5G06950.2	75.802	343	59	3	134	465	1	330	5.69e-178	501
Msa1321590	AT5G06950.4	75.802	343	59	3	134	465	1	330	5.69e-178	501
Msa1321590	AT5G06950.1	75.802	343	59	3	134	465	1	330	5.69e-178	501
Msa1321590	AT5G06950.5	75.802	343	59	3	134	465	1	330	5.69e-178	501
Msa1321590	AT5G06950.3	75.802	343	59	3	134	465	1	330	5.69e-178	501
Msa1321590	AT3G12250.3	82.566	304	52	1	162	465	22	324	9.11e-178	501
Msa1321590	AT5G06960.2	77.711	332	72	2	134	465	1	330	1.53e-173	490
Msa1321590	AT5G06960.1	77.711	332	72	2	134	465	1	330	1.53e-173	490
Msa1321590	AT5G06960.3	80.556	288	55	1	178	465	24	310	7.66e-163	462
Msa1321590	AT1G68640.1	69.552	335	99	2	133	465	119	452	5.23e-146	425
Msa1321590	AT1G08320.2	64.236	288	93	2	178	465	52	329	1.59e-130	382
Msa1321590	AT1G08320.5	64.236	288	93	2	178	465	52	329	1.59e-130	382
Msa1321590	AT1G08320.1	64.236	288	93	2	178	465	176	453	4.07e-129	383
Msa1321590	AT1G08320.4	64.236	288	93	2	178	465	176	453	4.07e-129	383
Msa1321590	AT1G08320.3	64.236	288	93	2	178	465	176	453	4.07e-129	383
Msa1321590	AT5G06839.2	60.968	310	107	4	169	464	106	415	1.37e-126	374
Msa1321590	AT5G06839.3	60.968	310	106	5	169	464	149	457	3.91e-126	374
Msa1321590	AT5G06839.1	60.968	310	106	5	169	464	106	414	4.39e-126	373
Msa1321590	AT1G22070.1	54.328	335	139	4	135	465	58	382	1.55e-114	342
Msa1321590	AT5G10030.5	58.054	298	112	3	173	465	73	362	2.89e-113	338
Msa1321590	AT5G10030.3	58.054	298	112	3	173	465	73	362	2.89e-113	338
Msa1321590	AT5G10030.1	58.054	298	112	3	173	465	73	362	2.89e-113	338
Msa1321590	AT5G10030.4	58.054	298	112	3	173	465	73	362	2.89e-113	338
Msa1321590	AT5G10030.2	58.054	298	112	3	173	465	73	362	2.89e-113	338
Msa1321590	AT1G77920.1	53.666	341	135	5	136	465	38	366	1.94e-111	333
Msa1321590	AT5G65210.5	50.140	357	152	5	127	465	18	366	4.60e-108	325
Msa1321590	AT5G65210.7	50.140	357	152	5	127	465	18	366	4.60e-108	325
Msa1321590	AT5G65210.3	50.140	357	152	5	127	465	18	366	4.60e-108	325
Msa1321590	AT5G65210.1	50.140	357	152	5	127	465	18	366	4.60e-108	325
Msa1321590	AT5G65210.2	50.140	357	152	5	127	465	18	366	4.60e-108	325
Msa1321590	AT5G65210.4	50.140	357	152	5	127	465	18	366	4.60e-108	325
Msa1321590	AT5G65210.6	56.376	298	117	3	173	465	7	296	5.37e-108	322
Msa1321590	AT1G58330.1	25.116	215	153	4	248	455	10	223	5.62e-12	65.9
Msa1321590	AT3G14880.2	22.791	215	124	3	255	444	17	214	6.15e-12	65.9
Msa1321590	AT3G14880.1	24.390	205	140	3	255	444	17	221	3.97e-11	63.5

Find 148 sgRNAs with CRISPR-Local

Find 388 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTGTATGAACACTTAATA+TGG	0.220209	8_4:-27651397	None:intergenic
CTGCTGAAAGGTGTTTCTTA+TGG	0.270813	8_4:+27653992	Msa1321590:CDS
AACGACTACTTTCCAATTGT+TGG	0.295766	8_4:-27652976	None:intergenic
TTGCTCTTAACAAGTGTTCT+TGG	0.297430	8_4:+27655102	Msa1321590:three_prime_UTR
CGGCCATGGCAAATTCTTCC+TGG	0.301627	8_4:-27651422	None:intergenic
CAATGCATCTTCTGCTTGTT+GGG	0.319617	8_4:-27654465	None:intergenic
GGCTCTGTTTGGTTCGTCAA+TGG	0.323135	8_4:-27651324	None:intergenic
TAGTCAACCATATCGCTTAG+AGG	0.358339	8_4:+27650475	Msa1321590:CDS
ACCTTCCTCATCTGGTTCCT+CGG	0.368179	8_4:+27654544	Msa1321590:CDS
GCTGAAGCTCTTGCTCTAGT+TGG	0.368756	8_4:-27653009	None:intergenic
TCCTTACTTTCTTTCTCAAC+CGG	0.370751	8_4:-27652184	None:intergenic
TTGTACTTGTAGTCTTTGTA+AGG	0.376294	8_4:+27655127	Msa1321590:three_prime_UTR
GCAACCTATGTGGCTCTGTT+TGG	0.379874	8_4:-27651335	None:intergenic
CCTTCCTCATCTGGTTCCTC+GGG	0.387610	8_4:+27654545	Msa1321590:CDS
ACTGGTGCACCTTCCTCATC+TGG	0.390580	8_4:+27654536	Msa1321590:CDS
ACAGCTTCTGGCAAATCAAT+TGG	0.390690	8_4:+27654391	Msa1321590:intron
GCTCTGTTTGGTTCGTCAAT+GGG	0.395776	8_4:-27651323	None:intergenic
ACATAGTCTAGGAATGGAAT+TGG	0.399932	8_4:+27655175	Msa1321590:three_prime_UTR
TATCATGACCAAAGTATTGA+TGG	0.401161	8_4:-27655075	None:intergenic
TCAAGAGAACTGGTATGAAC+AGG	0.403786	8_4:-27651162	None:intergenic
CAGGGCCAGGAGAAATTGTT+TGG	0.403898	8_4:-27651143	None:intergenic
ATAGGCCTATGTCCAACAAT+TGG	0.407086	8_4:+27652964	Msa1321590:intron
ACAATGCATCTTCTGCTTGT+TGG	0.407137	8_4:-27654466	None:intergenic
ACACATTCACTGAGTGGAAA+TGG	0.409133	8_4:+27653217	Msa1321590:CDS
AGCAAGAGCTTCAGCGAGCC+AGG	0.410136	8_4:+27653017	Msa1321590:CDS
TCCACATGCCAGACAACAAA+TGG	0.411800	8_4:-27653963	None:intergenic
ACAGCATCCTCTAAGCGATA+TGG	0.415546	8_4:-27650482	None:intergenic
CACTACAATCATTAATCCCT+TGG	0.416890	8_4:-27655013	None:intergenic
ATTGTCGTGTTGTCAATATT+CGG	0.418451	8_4:-27654916	None:intergenic
AGAAAGTAGAAAGACTTGTA+TGG	0.419423	8_4:-27648517	None:intergenic
CGCTCTCAGCTCTCTCTGGT+TGG	0.422468	8_4:+27654985	Msa1321590:CDS
AGCAGAAGATGCATTGTCTC+AGG	0.422669	8_4:+27654472	Msa1321590:CDS
TCTCACTGAGCAGCAGTTGA+TGG	0.427305	8_4:+27654418	Msa1321590:CDS
TCATACCAGTTCTCTTGATA+AGG	0.433339	8_4:+27651167	Msa1321590:CDS
AATATTGACAACACGACAAT+CGG	0.436484	8_4:+27654919	Msa1321590:CDS
GCTTCAGCGAGCCAGGCAAC+AGG	0.437362	8_4:+27653024	Msa1321590:CDS
TGCGCGCTCTCAGCTCTCTC+TGG	0.437796	8_4:+27654981	Msa1321590:CDS
CTCTCTGGTTGGCTCGTCCA+AGG	0.444712	8_4:+27654996	Msa1321590:CDS
TTTAACTCACTTAAAGTAAG+TGG	0.445667	8_4:+27651120	Msa1321590:CDS
TGAATTTATGAATTAAGTAC+AGG	0.454107	8_4:-27648576	None:intergenic
CTGAAGCTCTTGCTCTAGTT+GGG	0.454588	8_4:-27653008	None:intergenic
ACAAGAAGACATCTAGAGAT+TGG	0.455189	8_4:+27648484	Msa1321590:five_prime_UTR
GAACTTCGCATGATTGTTGA+TGG	0.456225	8_4:+27653877	Msa1321590:CDS
GCATTTGTTGTAGTGTCTGC+TGG	0.459312	8_4:-27654892	None:intergenic
ACTCACCTTATCAAGAGAAC+TGG	0.460871	8_4:-27651172	None:intergenic
TTTATCCTTTGATCTGTCAC+TGG	0.461119	8_4:-27652006	None:intergenic
CTTGCAGAGACGCTGTCCAC+TGG	0.462489	8_4:+27654518	Msa1321590:CDS
ATGAATTAAGTACAGGAATG+AGG	0.466028	8_4:-27648569	None:intergenic
GGCAAATTACATGGGTCAAA+TGG	0.469208	8_4:+27654574	Msa1321590:CDS
ATCAACAATCATGCGAAGTT+CGG	0.473485	8_4:-27653875	None:intergenic
GATGAGAAGGGAGCAGAAAG+AGG	0.473605	8_4:+27649447	Msa1321590:CDS
GGGGAATGTGGCAAATTACA+TGG	0.473681	8_4:+27654565	Msa1321590:CDS
GAAAGGTGTTTCTTATGGCT+TGG	0.475014	8_4:+27653997	Msa1321590:CDS
GGGTCAAATGGCTATGGCCA+TGG	0.476020	8_4:+27654586	Msa1321590:CDS
AGTTGGTGTTGAAGATGAGA+AGG	0.478818	8_4:+27649434	Msa1321590:CDS
ATCAATCACCATCAATACTT+TGG	0.479625	8_4:+27655067	Msa1321590:three_prime_UTR
GCTATGGCCATGGGTAAACT+TGG	0.487827	8_4:+27654596	Msa1321590:CDS
GCTGAAAGGTGTTGCAGCTA+AGG	0.488277	8_4:+27653930	Msa1321590:CDS
TGCAGAATATGCAAGGTGGC+TGG	0.488727	8_4:+27653792	Msa1321590:CDS
GTAGTTTGGGTTATATTTAG+CGG	0.489226	8_4:+27655281	Msa1321590:three_prime_UTR
AGATGCATTGTCTCAGGGTA+TGG	0.491210	8_4:+27654478	Msa1321590:CDS
TTCAACTTATATTGAGAGGC+GGG	0.495335	8_4:-27655313	None:intergenic
CCATGGCCGATGCCAGTCCT+AGG	0.497921	8_4:+27651436	Msa1321590:CDS
TCGGTCATCTGAACTTCTCA+AGG	0.499364	8_4:+27654026	Msa1321590:CDS
ATGAGAAGGGAGCAGAAAGA+GGG	0.507949	8_4:+27649448	Msa1321590:CDS
ATTGTCCATTGTAATTGTCA+TGG	0.508909	8_4:+27655233	Msa1321590:three_prime_UTR
TCTCTGGTTGGCTCGTCCAA+GGG	0.513960	8_4:+27654997	Msa1321590:CDS
CACCCGCTGATTCTTGTCAT+CGG	0.514566	8_4:-27651484	None:intergenic
TCTGTCGAAATATCAGTCCT+AGG	0.517561	8_4:-27651453	None:intergenic
ATCTGGTTCCTCGGGGAATG+TGG	0.518894	8_4:+27654553	Msa1321590:CDS
CCATTGTAATTGTCATGGAA+TGG	0.528907	8_4:+27655238	Msa1321590:three_prime_UTR
GGGAGTCTTCATATCAAGCT+CGG	0.529762	8_4:+27653186	Msa1321590:intron
CCTAGGACTGGCATCGGCCA+TGG	0.532193	8_4:-27651436	None:intergenic
CTCACTGAGCAGCAGTTGAT+GGG	0.536958	8_4:+27654419	Msa1321590:CDS
GAACTGGTATGAACAGGGCC+AGG	0.537901	8_4:-27651156	None:intergenic
AGGACTGATATTTCGACAGA+TGG	0.538631	8_4:+27651456	Msa1321590:CDS
TCCATTTGTTGTCTGGCATG+TGG	0.541899	8_4:+27653962	Msa1321590:CDS
GTGATGGTGGGAGTGTTGCA+TGG	0.541903	8_4:+27649398	Msa1321590:exon
CGAACCAAACAGAGCCACAT+AGG	0.549458	8_4:+27651331	Msa1321590:CDS
ACTTTCCAATTGTTGGACAT+AGG	0.549896	8_4:-27652969	None:intergenic
TGATGGTGGGAGTGTTGCAT+GGG	0.549942	8_4:+27649399	Msa1321590:exon
GAGTTCACATAGTCTAGGAA+TGG	0.550824	8_4:+27655169	Msa1321590:three_prime_UTR
AAAGGATAAATCAGATCAGA+AGG	0.550838	8_4:+27652019	Msa1321590:CDS
TGCATCTTCTGCTTGTTGGG+AGG	0.551942	8_4:-27654462	None:intergenic
ATCACAAGTCCATTGGAAAG+TGG	0.552426	8_4:+27648450	None:intergenic
GGTCAAATGGCTATGGCCAT+GGG	0.552555	8_4:+27654587	Msa1321590:CDS
ATCAGTCCTAGGACTGGCAT+CGG	0.552732	8_4:-27651442	None:intergenic
TTAGTTAATTGCAACCTATG+TGG	0.552953	8_4:-27651345	None:intergenic
GGAGTCTTCATATCAAGCTC+GGG	0.556167	8_4:+27653187	Msa1321590:CDS
CGAAATATCAGTCCTAGGAC+TGG	0.557940	8_4:-27651448	None:intergenic
AGGTGTTTCTTATGGCTTGG+TGG	0.558392	8_4:+27654000	Msa1321590:CDS
AAGCTTCAATCATCAAATCC+AGG	0.559884	8_4:+27651366	Msa1321590:CDS
TCCTAGACTATGTGAACTCT+AGG	0.561749	8_4:-27655165	None:intergenic
TCCTAGAGTTCACATAGTCT+AGG	0.561876	8_4:+27655164	Msa1321590:three_prime_UTR
CATAAGAAACACCTTTCAGC+AGG	0.562302	8_4:-27653991	None:intergenic
AGTAGAAAGACTTGTATGGA+TGG	0.563688	8_4:-27648513	None:intergenic
CGAGCTTCACTTCCGATGCA+TGG	0.564803	8_4:+27649475	Msa1321590:CDS
TTACATGGGTCAAATGGCTA+TGG	0.565231	8_4:+27654580	Msa1321590:CDS
CAGTGGACAGCGTCTCTGCA+AGG	0.565845	8_4:-27654517	None:intergenic
CACGATTCTGAGCGAGCCTG+CGG	0.566544	8_4:-27652145	None:intergenic
ACTGAATCTAACCTGTTGCC+TGG	0.566777	8_4:-27653035	None:intergenic
AGTGGCCAAACAATTTCTCC+TGG	0.570700	8_4:+27651138	Msa1321590:CDS
ATGGATTTCAGCTATACAGA+TGG	0.574550	8_4:+27650410	Msa1321590:intron
TTGGGAGGATTGTTGCAAGT+TGG	0.576668	8_4:-27654447	None:intergenic
CCGCGAAAAGTAATCATGGA+TGG	0.583482	8_4:-27654957	None:intergenic
AAGTTTCAACTTATATTGAG+AGG	0.588125	8_4:-27655317	None:intergenic
ACTCGAGGGCTTCATTCGAC+AGG	0.590463	8_4:+27654622	Msa1321590:CDS
ATCAACTGCTGCTCAGTGAG+AGG	0.591227	8_4:-27654416	None:intergenic
AAAAGTAATCATGGATGGCA+AGG	0.593869	8_4:-27654952	None:intergenic
AGTGGACAGCGTCTCTGCAA+GGG	0.594409	8_4:-27654516	None:intergenic
GGAATATGATGATGTGATGG+TGG	0.594577	8_4:+27649385	Msa1321590:five_prime_UTR
GAGGATGCTGTTGCTCTGAG+TGG	0.595978	8_4:+27650494	Msa1321590:CDS
TATTATTGTAGACTCTCCGC+AGG	0.596692	8_4:+27652129	Msa1321590:intron
CAATCATCAAATCCAGGTAG+CGG	0.596748	8_4:+27651372	Msa1321590:CDS
CTAAGAAATATACCATGCAT+CGG	0.599591	8_4:-27649487	None:intergenic
CACCGATGACAAGAATCAGC+GGG	0.600003	8_4:+27651482	Msa1321590:CDS
GAGTGTTCCAAGTTTACCCA+TGG	0.600742	8_4:-27654603	None:intergenic
TGACTCCAGTGACAGATCAA+AGG	0.603790	8_4:+27652001	Msa1321590:CDS
AAGTGTTCATACAGAAAACC+AGG	0.608836	8_4:+27651404	Msa1321590:CDS
ATTCCCCGAGGAACCAGATG+AGG	0.609365	8_4:-27654549	None:intergenic
GCAGAAGATGCATTGTCTCA+GGG	0.610171	8_4:+27654473	Msa1321590:CDS
CATGATTGTTGATGGTGTAG+TGG	0.611316	8_4:+27653885	Msa1321590:CDS
TGGATTTCAGCTATACAGAT+GGG	0.613061	8_4:+27650411	Msa1321590:intron
GCAGCCGCGAAAAGTAATCA+TGG	0.613549	8_4:-27654961	None:intergenic
AGATGAGGAAGGTGCACCAG+TGG	0.616659	8_4:-27654534	None:intergenic
GTTGGTGTTGAAGATGAGAA+GGG	0.617547	8_4:+27649435	Msa1321590:CDS
CCATTCCATGACAATTACAA+TGG	0.621913	8_4:-27655238	None:intergenic
GGTAAACTTGGAACACTCGA+GGG	0.626928	8_4:+27654608	Msa1321590:CDS
CATGCATCGGAAGTGAAGCT+CGG	0.629593	8_4:-27649474	None:intergenic
TTTCAACTTATATTGAGAGG+CGG	0.631750	8_4:-27655314	None:intergenic
CAAGAGAACTGGTATGAACA+GGG	0.632120	8_4:-27651161	None:intergenic
TGTGGAAAACACCTGCTGAA+AGG	0.636597	8_4:+27653980	Msa1321590:CDS
GGGTAAACTTGGAACACTCG+AGG	0.640334	8_4:+27654607	Msa1321590:CDS
ATGGGAAGTAGAATCAGAGT+TGG	0.641548	8_4:+27649417	Msa1321590:CDS
CCCGAGGAACCAGATGAGGA+AGG	0.644618	8_4:-27654545	None:intergenic
CAGGCTCGCTCAGAATCGTG+AGG	0.649410	8_4:+27652148	Msa1321590:CDS
AAACCAGGAAGAATTTGCCA+TGG	0.653768	8_4:+27651419	Msa1321590:CDS
AATTCGATGCAGAATATGCA+AGG	0.654839	8_4:+27653785	Msa1321590:CDS
TCTGATTATAGATTCAAACT+CGG	0.655868	8_4:+27651095	Msa1321590:intron
GAATATGATGATGTGATGGT+GGG	0.661147	8_4:+27649386	Msa1321590:five_prime_UTR
AGTGGAATATGATGATGTGA+TGG	0.663379	8_4:+27649382	Msa1321590:five_prime_UTR
GGGAATGTGGCAAATTACAT+GGG	0.666390	8_4:+27654566	Msa1321590:CDS
CTTCCTCATCTGGTTCCTCG+GGG	0.671637	8_4:+27654546	Msa1321590:CDS
GAACAAACACATTCACTGAG+TGG	0.681277	8_4:+27653211	Msa1321590:CDS
ACACCGATGACAAGAATCAG+CGG	0.687622	8_4:+27651481	Msa1321590:CDS
GCTCGCTCAGAATCGTGAGG+CGG	0.692281	8_4:+27652151	Msa1321590:CDS
GTAATTTGCCACATTCCCCG+AGG	0.720922	8_4:-27654561	None:intergenic
TCGATGCAGAATATGCAAGG+TGG	0.769831	8_4:+27653788	Msa1321590:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTATTATTTTATTTTA+TGG	+	chr8_4:27648689-27648708	Msa1321590:intron	0.0%
!!!	TTTTTATTTATTTTTACATC+TGG	+	chr8_4:27648845-27648864	Msa1321590:intron	10.0%
!!!	TTTTATTTATTTTTACATCT+GGG	+	chr8_4:27648846-27648865	Msa1321590:intron	10.0%
!!!	TTATCATTTTTTAAATAAAC+TGG	+	chr8_4:27650662-27650681	Msa1321590:intron	10.0%
!!!	TGATTTAAATAAATTGATTT+TGG	-	chr8_4:27652813-27652832	None:intergenic	10.0%
!!!	TAGTAAGATATTTTATAATA+GGG	-	chr8_4:27654828-27654847	None:intergenic	10.0%
!!!	ATAGTAAGATATTTTATAAT+AGG	-	chr8_4:27654829-27654848	None:intergenic	10.0%
!!	TATTATAAAATATCTTACTA+TGG	+	chr8_4:27654828-27654847	Msa1321590:intron	10.0%
!!!	TTTAACTTTCTTATATCTTT+TGG	+	chr8_4:27649726-27649745	Msa1321590:intron	15.0%
!!	TCAAAATATATGTTATAGTT+TGG	+	chr8_4:27650122-27650141	Msa1321590:intron	15.0%
!!	TTAAAGATAATAAATATGGT+TGG	-	chr8_4:27652719-27652738	None:intergenic	15.0%
!!!	TAATTTTTTACAACTATCAT+AGG	-	chr8_4:27652882-27652901	None:intergenic	15.0%
!!	AACATAACAAAAACAAATTA+TGG	-	chr8_4:27653689-27653708	None:intergenic	15.0%
!!	TATTAAAAAAGGAATAGTAA+AGG	+	chr8_4:27654226-27654245	Msa1321590:intron	15.0%
!!	TGAATTTATGAATTAAGTAC+AGG	-	chr8_4:27648579-27648598	None:intergenic	20.0%
!!	AACACAAATATCTATCATAA+AGG	-	chr8_4:27649011-27649030	None:intergenic	20.0%
!!!	ATTTGTTTTGTACTATGTTT+TGG	+	chr8_4:27649128-27649147	Msa1321590:intron	20.0%
!!!	ATTTCAATTTTTAGATTGCA+AGG	+	chr8_4:27649516-27649535	Msa1321590:intron	20.0%
!!!	TTGCCTTAAATTTAGATTTT+GGG	+	chr8_4:27649577-27649596	Msa1321590:intron	20.0%
!!!	TTTTCATATAAGATGTAAGT+CGG	-	chr8_4:27649703-27649722	None:intergenic	20.0%
!!!	ATGTTAAATAGCTTTTCTAA+AGG	-	chr8_4:27650066-27650085	None:intergenic	20.0%
!!!	AAGGAAATTGTTTTATTTTG+AGG	+	chr8_4:27650088-27650107	Msa1321590:intron	20.0%
!!!	TTTTATTTTGAGGAGTATAA+TGG	+	chr8_4:27650098-27650117	Msa1321590:intron	20.0%
!!!	TTTCTCTAAAATGAAATGTA+TGG	+	chr8_4:27650362-27650381	Msa1321590:intron	20.0%
!!	AGATGTTATCATTTAAACAA+GGG	-	chr8_4:27651538-27651557	None:intergenic	20.0%
!!	ATATTAACAGAATTATTGCA+TGG	+	chr8_4:27652048-27652067	Msa1321590:intron	20.0%
!!	ATTATACGATAGATACTAAT+TGG	-	chr8_4:27652089-27652108	None:intergenic	20.0%
!!	ATGATAACAACATTTATATG+GGG	-	chr8_4:27652273-27652292	None:intergenic	20.0%
!!	CATGATAACAACATTTATAT+GGG	-	chr8_4:27652274-27652293	None:intergenic	20.0%
!!	ACATGATAACAACATTTATA+TGG	-	chr8_4:27652275-27652294	None:intergenic	20.0%
!!	AGTTTGTACAAAAAACTATT+CGG	+	chr8_4:27652329-27652348	Msa1321590:intron	20.0%
!!!	AAGTCAATAATTTTAACATG+AGG	+	chr8_4:27652431-27652450	Msa1321590:intron	20.0%
!!!	AGTCAATAATTTTAACATGA+GGG	+	chr8_4:27652432-27652451	Msa1321590:intron	20.0%
!!!	CAAAGTATATTCATTTTCAT+AGG	+	chr8_4:27652946-27652965	Msa1321590:intron	20.0%
!!	TGACAAAAGAAAACTTTAAA+TGG	+	chr8_4:27653429-27653448	Msa1321590:intron	20.0%
!!	ACACAATTAAATGATTGTTA+TGG	-	chr8_4:27653751-27653770	None:intergenic	20.0%
!!	TTCAAAATAAGTCAAAGATT+CGG	-	chr8_4:27654143-27654162	None:intergenic	20.0%
!!	AAACTTGTGTTCAATTAATT+TGG	+	chr8_4:27654661-27654680	Msa1321590:intron	20.0%
!!!	ATTTTTGTGTTTTTGTTACA+AGG	+	chr8_4:27654856-27654875	Msa1321590:intron	20.0%
!	TCGTCATCAAAAAAATTTCA+AGG	+	chr8_4:27648623-27648642	Msa1321590:five_prime_UTR	25.0%
!	TCATCAAAAAAATTTCAAGG+TGG	+	chr8_4:27648626-27648645	Msa1321590:intron	25.0%
!	CATCAAAAAAATTTCAAGGT+GGG	+	chr8_4:27648627-27648646	Msa1321590:intron	25.0%
!	AATAAAAACCATCATGTTCA+TGG	+	chr8_4:27648725-27648744	Msa1321590:intron	25.0%
!	TTAGTTCAACTTAGTATAAC+AGG	+	chr8_4:27648754-27648773	Msa1321590:intron	25.0%
!	ATATTTGTGTTGCAACTAAA+GGG	+	chr8_4:27649020-27649039	Msa1321590:intron	25.0%
!!	AATTCAGTTTATGTTTTACC+AGG	+	chr8_4:27649082-27649101	Msa1321590:intron	25.0%
!	TCTGTTGTGTTTATTGTAAT+TGG	+	chr8_4:27649162-27649181	Msa1321590:intron	25.0%
!!	AATTCACTTCATGGTTTTTA+AGG	+	chr8_4:27649185-27649204	Msa1321590:intron	25.0%
!!!	TTGATTTGTTGAGATTTATG+AGG	+	chr8_4:27649254-27649273	Msa1321590:intron	25.0%
!!!	TTTGTTGTTTGTTTTGTGTT+AGG	+	chr8_4:27649328-27649347	Msa1321590:intron	25.0%
!!!	TTGTTGTTTGTTTTGTGTTA+GGG	+	chr8_4:27649329-27649348	Msa1321590:intron	25.0%
!	CAAGGTGAAATATAGATTTA+GGG	+	chr8_4:27649534-27649553	Msa1321590:intron	25.0%
!!	GTTGCCTTAAATTTAGATTT+TGG	+	chr8_4:27649576-27649595	Msa1321590:intron	25.0%
!	AAGCCCAAAATCTAAATTTA+AGG	-	chr8_4:27649583-27649602	None:intergenic	25.0%
!!!	CTTATTTTGCATAAATCTCT+AGG	+	chr8_4:27649664-27649683	Msa1321590:intron	25.0%
!	GAAAAGCTATTTAACATACA+AGG	+	chr8_4:27650069-27650088	Msa1321590:intron	25.0%
!!!	GAAAAAGTTTTGTTACTACT+AGG	+	chr8_4:27650203-27650222	Msa1321590:intron	25.0%
!!!	TAGATTTGTTGATCGAAATT+TGG	+	chr8_4:27650229-27650248	Msa1321590:intron	25.0%
!	ATCCTAATTCTGACAAAATA+TGG	+	chr8_4:27650391-27650410	Msa1321590:intron	25.0%
!!	ATCCATATTTTGTCAGAATT+AGG	-	chr8_4:27650396-27650415	None:intergenic	25.0%
!!!	AATTTTCATGTTTCTGACTT+TGG	+	chr8_4:27650446-27650465	Msa1321590:CDS	25.0%
!!!	AAAAAGTTTTATGAGACTCT+TGG	-	chr8_4:27650611-27650630	None:intergenic	25.0%
!	TCGTTTAGACAAGTAAAAAA+AGG	-	chr8_4:27650635-27650654	None:intergenic	25.0%
!	AATAAACTGGAACTATACAT+TGG	+	chr8_4:27650675-27650694	Msa1321590:intron	25.0%
!	AAAGATAGACAATGATATCT+AGG	-	chr8_4:27651014-27651033	None:intergenic	25.0%
!	AAAATTCATTAGCAAAGGAA+AGG	-	chr8_4:27651075-27651094	None:intergenic	25.0%
!	TCAGAAAAATTCATTAGCAA+AGG	-	chr8_4:27651080-27651099	None:intergenic	25.0%
!	TCTGATTATAGATTCAAACT+CGG	+	chr8_4:27651095-27651114	Msa1321590:intron	25.0%
!	TTTAACTCACTTAAAGTAAG+TGG	+	chr8_4:27651120-27651139	Msa1321590:CDS	25.0%
!!!	ATGTTGAAGTTTTAGCATTA+CGG	+	chr8_4:27651239-27651258	Msa1321590:intron	25.0%
!	TTCTGTATGAACACTTAATA+TGG	-	chr8_4:27651400-27651419	None:intergenic	25.0%
!	GAGATGTTATCATTTAAACA+AGG	-	chr8_4:27651539-27651558	None:intergenic	25.0%
!	ATAAGTTGTTTATCCAAACA+GGG	+	chr8_4:27651794-27651813	Msa1321590:intron	25.0%
!!!	CATCTTATTAAGGCTTTATT+TGG	-	chr8_4:27651888-27651907	None:intergenic	25.0%
!	GTTTGTACAAAAAACTATTC+GGG	+	chr8_4:27652330-27652349	Msa1321590:intron	25.0%
!!!	GTCAATAATTTTAACATGAG+GGG	+	chr8_4:27652433-27652452	Msa1321590:intron	25.0%
!!!	TCAATAATTTTAACATGAGG+GGG	+	chr8_4:27652434-27652453	Msa1321590:intron	25.0%
!	AAACATGCATTAAACATTAC+AGG	-	chr8_4:27652556-27652575	None:intergenic	25.0%
!!	CATGTTTGAATTATTTTCGA+GGG	+	chr8_4:27652569-27652588	Msa1321590:intron	25.0%
!	AAGATAATAAATATGGTTGG+AGG	-	chr8_4:27652716-27652735	None:intergenic	25.0%
!	GAAAATGAATATACTTTGTC+AGG	-	chr8_4:27652944-27652963	None:intergenic	25.0%
!!	AATTTCTTGTTTCTTCTTGT+AGG	+	chr8_4:27653165-27653184	Msa1321590:intron	25.0%
!!	ATTTCTTGTTTCTTCTTGTA+GGG	+	chr8_4:27653166-27653185	Msa1321590:intron	25.0%
!	TGAACTAGAAATATGTCTAA+TGG	+	chr8_4:27653277-27653296	Msa1321590:intron	25.0%
!	ACAGGAAAATGATTAAAATG+TGG	+	chr8_4:27653589-27653608	Msa1321590:intron	25.0%
!	CAATCATTTAATTGTGTTTC+AGG	+	chr8_4:27653754-27653773	Msa1321590:intron	25.0%
!!	CACATTATGATGAGATTTTT+AGG	+	chr8_4:27653908-27653927	Msa1321590:CDS	25.0%
!	AATGTGATCCATATTGAAAA+GGG	-	chr8_4:27654066-27654085	None:intergenic	25.0%
!	TCAAAATAAGTCAAAGATTC+GGG	-	chr8_4:27654142-27654161	None:intergenic	25.0%
!	TTAAGAACAAGAACTTTCTT+TGG	+	chr8_4:27654182-27654201	Msa1321590:intron	25.0%
!	TGGGAGTTTAGTATTAAAAA+AGG	+	chr8_4:27654215-27654234	Msa1321590:intron	25.0%
!!!	ACCGAGTTTTAATATTTTGA+AGG	+	chr8_4:27654310-27654329	Msa1321590:intron	25.0%
!	GCCTTCAAAATATTAAAACT+CGG	-	chr8_4:27654314-27654333	None:intergenic	25.0%
!!	ATTAATTTGGTACATTTGCA+TGG	+	chr8_4:27654674-27654693	Msa1321590:intron	25.0%
!	AAGTTTCAACTTATATTGAG+AGG	-	chr8_4:27655320-27655339	None:intergenic	25.0%
	AGAAAGTAGAAAGACTTGTA+TGG	-	chr8_4:27648520-27648539	None:intergenic	30.0%
	ATGAATTAAGTACAGGAATG+AGG	-	chr8_4:27648572-27648591	None:intergenic	30.0%
!	AAAAAATTTCAAGGTGGGTT+TGG	+	chr8_4:27648632-27648651	Msa1321590:intron	30.0%
	AAAATTCACCATGAACATGA+TGG	-	chr8_4:27648736-27648755	None:intergenic	30.0%
!!	TTTGATCTTGTTGATGATGT+TGG	+	chr8_4:27648790-27648809	Msa1321590:intron	30.0%
!	TTGACTTTTGAGCACTTATT+TGG	+	chr8_4:27648920-27648939	Msa1321590:intron	30.0%
!	TATCATAAAGGCACAAACTT+TGG	-	chr8_4:27648999-27649018	None:intergenic	30.0%
	GATATTTGTGTTGCAACTAA+AGG	+	chr8_4:27649019-27649038	Msa1321590:intron	30.0%
	CCAAAACATAAATTTCAACC+TGG	-	chr8_4:27649103-27649122	None:intergenic	30.0%
!!	CCAGGTTGAAATTTATGTTT+TGG	+	chr8_4:27649100-27649119	Msa1321590:intron	30.0%
	TGTAATTGGAATTCACTTCA+TGG	+	chr8_4:27649176-27649195	Msa1321590:intron	30.0%
	CTAAGAAATATACCATGCAT+CGG	-	chr8_4:27649490-27649509	None:intergenic	30.0%
	GCAAGGTGAAATATAGATTT+AGG	+	chr8_4:27649533-27649552	Msa1321590:intron	30.0%
!!	ATTTAGATTTTGGGCTTGTT+TGG	+	chr8_4:27649586-27649605	Msa1321590:intron	30.0%
!	GTTTGGATTGACTTATTTAC+TGG	+	chr8_4:27649603-27649622	Msa1321590:intron	30.0%
!!	TAAATCTCTAGGATAGCTTA+TGG	+	chr8_4:27649675-27649694	Msa1321590:intron	30.0%
	AATAATTGTCATAGTCCAGT+TGG	-	chr8_4:27649847-27649866	None:intergenic	30.0%
	TTAGGTTAAACCTTATCATG+TGG	+	chr8_4:27649964-27649983	Msa1321590:intron	30.0%
	TATACAATCTCCACATGATA+AGG	-	chr8_4:27649977-27649996	None:intergenic	30.0%
	TCATTGTATTGTATGTAACG+TGG	+	chr8_4:27650017-27650036	Msa1321590:intron	30.0%
	CATTGTATTGTATGTAACGT+GGG	+	chr8_4:27650018-27650037	Msa1321590:intron	30.0%
	ATTGTATTGTATGTAACGTG+GGG	+	chr8_4:27650019-27650038	Msa1321590:intron	30.0%
!	TACTTAGACGTGTTTTCTTT+GGG	+	chr8_4:27650282-27650301	Msa1321590:intron	30.0%
!	ACAACTACTTGTTGATTACT+TGG	+	chr8_4:27650722-27650741	Msa1321590:intron	30.0%
	TTGTCAAATGGAAACTATAG+TGG	-	chr8_4:27650877-27650896	None:intergenic	30.0%
!!	AAGTACAAAGTGTTGTCAAA+TGG	-	chr8_4:27650889-27650908	None:intergenic	30.0%
	TTGTACTTAATAGCATATCG+TGG	+	chr8_4:27650901-27650920	Msa1321590:intron	30.0%
!	ATTCTAGTGTTGTTGAATAG+CGG	-	chr8_4:27650980-27650999	None:intergenic	30.0%
	TTAGTTAATTGCAACCTATG+TGG	-	chr8_4:27651348-27651367	None:intergenic	30.0%
!	CAAGTTGAACTTGTCAAATA+AGG	+	chr8_4:27651597-27651616	Msa1321590:intron	30.0%
!	TAACTATGTTGCATAGCTTT+TGG	-	chr8_4:27651682-27651701	None:intergenic	30.0%
	CATAAGTTGTTTATCCAAAC+AGG	+	chr8_4:27651793-27651812	Msa1321590:intron	30.0%
	AAAGGATAAATCAGATCAGA+AGG	+	chr8_4:27652019-27652038	Msa1321590:CDS	30.0%
!	ATGTCGGTTACAAAGTTATA+AGG	-	chr8_4:27652363-27652382	None:intergenic	30.0%
!	CAAAAAGTGTAACTTCCTAT+TGG	-	chr8_4:27652389-27652408	None:intergenic	30.0%
!	CAATAGGAAGTTACACTTTT+TGG	+	chr8_4:27652387-27652406	Msa1321590:intron	30.0%
!!	CAATAATTTTAACATGAGGG+GGG	+	chr8_4:27652435-27652454	Msa1321590:intron	30.0%
!!	AATAATTTTAACATGAGGGG+GGG	+	chr8_4:27652436-27652455	Msa1321590:intron	30.0%
!	GCATGTTTGAATTATTTTCG+AGG	+	chr8_4:27652568-27652587	Msa1321590:intron	30.0%
	AATGTGAAGTGATCTATGAT+TGG	-	chr8_4:27652776-27652795	None:intergenic	30.0%
	CAAGAAGAAACAAGAAATTC+AGG	-	chr8_4:27653164-27653183	None:intergenic	30.0%
!!	CATATTTCTAGTTCAAACCA+AGG	-	chr8_4:27653272-27653291	None:intergenic	30.0%
!!	AGTAGATTTGAGGTATTGTT+TGG	+	chr8_4:27653381-27653400	Msa1321590:intron	30.0%
!!	TTTGAGGTATTGTTTGGTAT+AGG	+	chr8_4:27653387-27653406	Msa1321590:intron	30.0%
	GAATGTGATCCATATTGAAA+AGG	-	chr8_4:27654067-27654086	None:intergenic	30.0%
!	TTCCTTTTTATGCTGATACT+CGG	+	chr8_4:27654084-27654103	Msa1321590:intron	30.0%
!	TTCAATTGATTTCAAGGAAC+AGG	-	chr8_4:27654118-27654137	None:intergenic	30.0%
	CAAAATAAGTCAAAGATTCG+GGG	-	chr8_4:27654141-27654160	None:intergenic	30.0%
	TGAGATATGTATTTAACAGC+AGG	-	chr8_4:27654279-27654298	None:intergenic	30.0%
	AAGTTCATAACCTAAAGTGT+CGG	-	chr8_4:27654741-27654760	None:intergenic	30.0%
	ATTGTCGTGTTGTCAATATT+CGG	-	chr8_4:27654919-27654938	None:intergenic	30.0%
	AATATTGACAACACGACAAT+CGG	+	chr8_4:27654919-27654938	Msa1321590:CDS	30.0%
	ATCAATCACCATCAATACTT+TGG	+	chr8_4:27655067-27655086	Msa1321590:three_prime_UTR	30.0%
!!	TATCATGACCAAAGTATTGA+TGG	-	chr8_4:27655078-27655097	None:intergenic	30.0%
	TTGTACTTGTAGTCTTTGTA+AGG	+	chr8_4:27655127-27655146	Msa1321590:three_prime_UTR	30.0%
	ATTGTCCATTGTAATTGTCA+TGG	+	chr8_4:27655233-27655252	Msa1321590:three_prime_UTR	30.0%
!!	TTTTTCGAACAAAGGTAGTT+TGG	+	chr8_4:27655267-27655286	Msa1321590:three_prime_UTR	30.0%
!	TTTTCGAACAAAGGTAGTTT+GGG	+	chr8_4:27655268-27655287	Msa1321590:three_prime_UTR	30.0%
!	GTAGTTTGGGTTATATTTAG+CGG	+	chr8_4:27655281-27655300	Msa1321590:three_prime_UTR	30.0%
	TTTCAACTTATATTGAGAGG+CGG	-	chr8_4:27655317-27655336	None:intergenic	30.0%
	ACAAGAAGACATCTAGAGAT+TGG	+	chr8_4:27648484-27648503	Msa1321590:five_prime_UTR	35.0%
	AGTAGAAAGACTTGTATGGA+TGG	-	chr8_4:27648516-27648535	None:intergenic	35.0%
	ATGAGGAAAAAGATAGACAG+AGG	-	chr8_4:27648555-27648574	None:intergenic	35.0%
!!	CTTGTTGATGATGTTGGATT+TGG	+	chr8_4:27648796-27648815	Msa1321590:intron	35.0%
!!	TAACTGAGTTGGTGTCAAAT+TGG	+	chr8_4:27648871-27648890	Msa1321590:intron	35.0%
	TGTCTTTGTGTTCTTGTGAT+TGG	+	chr8_4:27649059-27649078	Msa1321590:intron	35.0%
	GTTGAGATTTATGAGGTTGT+TGG	+	chr8_4:27649261-27649280	Msa1321590:intron	35.0%
!	GATTTATGAGGTTGTTGGTT+TGG	+	chr8_4:27649266-27649285	Msa1321590:intron	35.0%
!	TACATTTTGTTGAGAAGCAG+TGG	+	chr8_4:27649364-27649383	Msa1321590:five_prime_UTR	35.0%
!	AGTGGAATATGATGATGTGA+TGG	+	chr8_4:27649382-27649401	Msa1321590:five_prime_UTR	35.0%
!	GAATATGATGATGTGATGGT+GGG	+	chr8_4:27649386-27649405	Msa1321590:five_prime_UTR	35.0%
	TCACTGTGAATATCTTCCTT+GGG	+	chr8_4:27649938-27649957	Msa1321590:intron	35.0%
!!	TTATAGTTTGGCTCTAGTCT+TGG	+	chr8_4:27650134-27650153	Msa1321590:intron	35.0%
	TTGTTGATCGAAATTTGGCA+TGG	+	chr8_4:27650234-27650253	Msa1321590:intron	35.0%
!	GTACTTAGACGTGTTTTCTT+TGG	+	chr8_4:27650281-27650300	Msa1321590:intron	35.0%
!	ACTTAGACGTGTTTTCTTTG+GGG	+	chr8_4:27650283-27650302	Msa1321590:intron	35.0%
	ATGGATTTCAGCTATACAGA+TGG	+	chr8_4:27650410-27650429	Msa1321590:intron	35.0%
	TGGATTTCAGCTATACAGAT+GGG	+	chr8_4:27650411-27650430	Msa1321590:intron	35.0%
	ACATGTAAACTTTGCCAAGT+CGG	-	chr8_4:27650539-27650558	None:intergenic	35.0%
	TGTTGTATTATCTCGTGTCT+TGG	+	chr8_4:27650581-27650600	Msa1321590:intron	35.0%
	TGTGAATGTGTGTTTGTAGT+TGG	-	chr8_4:27650776-27650795	None:intergenic	35.0%
	TTCATTAGCAAAGGAAAGGA+TGG	-	chr8_4:27651071-27651090	None:intergenic	35.0%
	TCATACCAGTTCTCTTGATA+AGG	+	chr8_4:27651167-27651186	Msa1321590:CDS	35.0%
	TTCCAATGAATTTCCTGCTT+CGG	+	chr8_4:27651211-27651230	Msa1321590:intron	35.0%
	TTCAACATTTATACCGAAGC+AGG	-	chr8_4:27651227-27651246	None:intergenic	35.0%
	ATGTTGGCAACTAAAGAGAA+AGG	-	chr8_4:27651290-27651309	None:intergenic	35.0%
	AAGCTTCAATCATCAAATCC+AGG	+	chr8_4:27651366-27651385	Msa1321590:CDS	35.0%
	AAGTGTTCATACAGAAAACC+AGG	+	chr8_4:27651404-27651423	Msa1321590:CDS	35.0%
!	ACAAGGGAGAAAAAGCATAA+AGG	-	chr8_4:27651522-27651541	None:intergenic	35.0%
	TTGACAAGTTCAACTTGAAC+TGG	-	chr8_4:27651594-27651613	None:intergenic	35.0%
	AAGTGCTTATGCATAAGCTA+CGG	-	chr8_4:27651820-27651839	None:intergenic	35.0%
	ATGCGCTACTCATCTTATTA+AGG	-	chr8_4:27651898-27651917	None:intergenic	35.0%
	TTTATCCTTTGATCTGTCAC+TGG	-	chr8_4:27652009-27652028	None:intergenic	35.0%
	TCCTTACTTTCTTTCTCAAC+CGG	-	chr8_4:27652187-27652206	None:intergenic	35.0%
	AACTTCCTATTGGACAATGT+CGG	-	chr8_4:27652379-27652398	None:intergenic	35.0%
	TGGTCTTCCTTCAGTTAAAT+TGG	+	chr8_4:27652407-27652426	Msa1321590:intron	35.0%
!	TGACTTGCCAATTTAACTGA+AGG	-	chr8_4:27652417-27652436	None:intergenic	35.0%
	CAGGTCATATGATTCTTCAA+CGG	-	chr8_4:27652537-27652556	None:intergenic	35.0%
	CTTCAAGTCAAAACTCATTC+TGG	-	chr8_4:27652674-27652693	None:intergenic	35.0%
	ACGTGTGTTTGATTCAATGA+TGG	-	chr8_4:27652843-27652862	None:intergenic	35.0%
	AACACACGTGTATACTTCTT+TGG	+	chr8_4:27652854-27652873	Msa1321590:intron	35.0%
	TGCAATTCAATGGTCAATTG+AGG	-	chr8_4:27652918-27652937	None:intergenic	35.0%
	ACTTTCCAATTGTTGGACAT+AGG	-	chr8_4:27652972-27652991	None:intergenic	35.0%
	AACGACTACTTTCCAATTGT+TGG	-	chr8_4:27652979-27652998	None:intergenic	35.0%
	CGTGTCGTACAAAAATCAAA+AGG	-	chr8_4:27653112-27653131	None:intergenic	35.0%
	AAAATAGAGTACGACTGCTA+AGG	-	chr8_4:27653138-27653157	None:intergenic	35.0%
!	GAACTATGTTACTTGTTCCT+TGG	+	chr8_4:27653252-27653271	Msa1321590:intron	35.0%
	AATATGTCTAATGGTCTGTC+TGG	+	chr8_4:27653286-27653305	Msa1321590:intron	35.0%
	GAAAGTAAGAAGAGAGAAGT+AGG	+	chr8_4:27653318-27653337	Msa1321590:intron	35.0%
	AACGAACCACCATTTATGAA+AGG	+	chr8_4:27653561-27653580	Msa1321590:intron	35.0%
	ACGAACCACCATTTATGAAA+GGG	+	chr8_4:27653562-27653581	Msa1321590:intron	35.0%
	TTGTACCCTTTCATAAATGG+TGG	-	chr8_4:27653570-27653589	None:intergenic	35.0%
	CTGTTGTACCCTTTCATAAA+TGG	-	chr8_4:27653573-27653592	None:intergenic	35.0%
!	CATTTATGAAAGGGTACAAC+AGG	+	chr8_4:27653571-27653590	Msa1321590:intron	35.0%
!	ATTATGGTGTTTTACGCTGA+TGG	-	chr8_4:27653673-27653692	None:intergenic	35.0%
	AATTCGATGCAGAATATGCA+AGG	+	chr8_4:27653785-27653804	Msa1321590:CDS	35.0%
	ATCAACAATCATGCGAAGTT+CGG	-	chr8_4:27653878-27653897	None:intergenic	35.0%
!!	GATGAGATTTTTAGGCTGAA+AGG	+	chr8_4:27653916-27653935	Msa1321590:CDS	35.0%
!!!	GATGTTTTCCATTTGTTGTC+TGG	+	chr8_4:27653955-27653974	Msa1321590:CDS	35.0%
!	TATGCTGACCCTTTTCAATA+TGG	+	chr8_4:27654055-27654074	Msa1321590:intron	35.0%
	TGTATTTAACAGCAGGCTTA+GGG	-	chr8_4:27654272-27654291	None:intergenic	35.0%
	ATGTATTTAACAGCAGGCTT+AGG	-	chr8_4:27654273-27654292	None:intergenic	35.0%
!	TGGTACATTTGCATGGTTTT+TGG	+	chr8_4:27654681-27654700	Msa1321590:intron	35.0%
	AAAAGTAATCATGGATGGCA+AGG	-	chr8_4:27654955-27654974	None:intergenic	35.0%
	CACTACAATCATTAATCCCT+TGG	-	chr8_4:27655016-27655035	None:intergenic	35.0%
	TTGCTCTTAACAAGTGTTCT+TGG	+	chr8_4:27655102-27655121	Msa1321590:three_prime_UTR	35.0%
	ACATAGTCTAGGAATGGAAT+TGG	+	chr8_4:27655175-27655194	Msa1321590:three_prime_UTR	35.0%
	CCATTCCATGACAATTACAA+TGG	-	chr8_4:27655241-27655260	None:intergenic	35.0%
	CCATTGTAATTGTCATGGAA+TGG	+	chr8_4:27655238-27655257	Msa1321590:three_prime_UTR	35.0%
!!	GGCTTTGATTTTTCGAACAA+AGG	+	chr8_4:27655259-27655278	Msa1321590:three_prime_UTR	35.0%
	TTCAACTTATATTGAGAGGC+GGG	-	chr8_4:27655316-27655335	None:intergenic	35.0%
!!!	GTTCTTGTTTTGCGTGTGTT+TGG	+	chr8_4:27648951-27648970	Msa1321590:intron	40.0%
!!!	TTTTGCGTGTGTTTGGTTTG+TGG	+	chr8_4:27648958-27648977	Msa1321590:intron	40.0%
!!	TTTGCGTGTGTTTGGTTTGT+GGG	+	chr8_4:27648959-27648978	Msa1321590:intron	40.0%
!	GGAATATGATGATGTGATGG+TGG	+	chr8_4:27649385-27649404	Msa1321590:five_prime_UTR	40.0%
	ATGGGAAGTAGAATCAGAGT+TGG	+	chr8_4:27649417-27649436	Msa1321590:CDS	40.0%
!!	AGTTGGTGTTGAAGATGAGA+AGG	+	chr8_4:27649434-27649453	Msa1321590:CDS	40.0%
!!	GTTGGTGTTGAAGATGAGAA+GGG	+	chr8_4:27649435-27649454	Msa1321590:CDS	40.0%
	TAGTCCAGTTGGTCCATATA+AGG	-	chr8_4:27649836-27649855	None:intergenic	40.0%
	GTCACTGTGAATATCTTCCT+TGG	+	chr8_4:27649937-27649956	Msa1321590:intron	40.0%
	AATATCTTCCTTGGGTGGTT+AGG	+	chr8_4:27649946-27649965	Msa1321590:intron	40.0%
	TATGCAGCTAGTTGTATGAC+AGG	+	chr8_4:27650181-27650200	Msa1321590:intron	40.0%
	TCGAAATTTGGCATGGTTCA+TGG	+	chr8_4:27650241-27650260	Msa1321590:intron	40.0%
	TAGTCAACCATATCGCTTAG+AGG	+	chr8_4:27650475-27650494	Msa1321590:CDS	40.0%
	AGTCTTCTTGTAATCCGACT+TGG	+	chr8_4:27650522-27650541	Msa1321590:intron	40.0%
	TTGCAGATCGCAGAAAATAG+TGG	+	chr8_4:27650815-27650834	Msa1321590:intron	40.0%
	TGCAGATCGCAGAAAATAGT+GGG	+	chr8_4:27650816-27650835	Msa1321590:intron	40.0%
	CAAGAGAACTGGTATGAACA+GGG	-	chr8_4:27651164-27651183	None:intergenic	40.0%
	TCAAGAGAACTGGTATGAAC+AGG	-	chr8_4:27651165-27651184	None:intergenic	40.0%
	ACTCACCTTATCAAGAGAAC+TGG	-	chr8_4:27651175-27651194	None:intergenic	40.0%
	TACCGAAGCAGGAAATTCAT+TGG	-	chr8_4:27651216-27651235	None:intergenic	40.0%
	CAATCATCAAATCCAGGTAG+CGG	+	chr8_4:27651372-27651391	Msa1321590:CDS	40.0%
	AAACCAGGAAGAATTTGCCA+TGG	+	chr8_4:27651419-27651438	Msa1321590:CDS	40.0%
	TCTGTCGAAATATCAGTCCT+AGG	-	chr8_4:27651456-27651475	None:intergenic	40.0%
	AGGACTGATATTTCGACAGA+TGG	+	chr8_4:27651456-27651475	Msa1321590:CDS	40.0%
	TATTATTGTAGACTCTCCGC+AGG	+	chr8_4:27652129-27652148	Msa1321590:intron	40.0%
	TGTAACCGACATTGTCCAAT+AGG	+	chr8_4:27652371-27652390	Msa1321590:intron	40.0%
	CAAGTCAAAACTCATTCTGG+AGG	-	chr8_4:27652671-27652690	None:intergenic	40.0%
	TGTCAGGTCTTGCAATTCAA+TGG	-	chr8_4:27652928-27652947	None:intergenic	40.0%
	ATAGGCCTATGTCCAACAAT+TGG	+	chr8_4:27652964-27652983	Msa1321590:intron	40.0%
	ACTGGTGCAATCTAATTGCA+AGG	-	chr8_4:27653065-27653084	None:intergenic	40.0%
	GAACAAACACATTCACTGAG+TGG	+	chr8_4:27653211-27653230	Msa1321590:CDS	40.0%
	ACACATTCACTGAGTGGAAA+TGG	+	chr8_4:27653217-27653236	Msa1321590:CDS	40.0%
	TGAGCAGTGTAGTAGATTTG+AGG	+	chr8_4:27653371-27653390	Msa1321590:intron	40.0%
	GAACTTCGCATGATTGTTGA+TGG	+	chr8_4:27653877-27653896	Msa1321590:CDS	40.0%
!!	CATGATTGTTGATGGTGTAG+TGG	+	chr8_4:27653885-27653904	Msa1321590:CDS	40.0%
	CATAAGAAACACCTTTCAGC+AGG	-	chr8_4:27653994-27654013	None:intergenic	40.0%
!!	CTGCTGAAAGGTGTTTCTTA+TGG	+	chr8_4:27653992-27654011	Msa1321590:CDS	40.0%
!!	GAAAGGTGTTTCTTATGGCT+TGG	+	chr8_4:27653997-27654016	Msa1321590:CDS	40.0%
!!!	TCTTATGGCTTGGTGGTTTT+CGG	+	chr8_4:27654007-27654026	Msa1321590:CDS	40.0%
	AGCCGAGTATCAGCATAAAA+AGG	-	chr8_4:27654089-27654108	None:intergenic	40.0%
!	TCGGGGTTCAATTGATTTCA+AGG	-	chr8_4:27654124-27654143	None:intergenic	40.0%
	AACAAGAACTTTCTTTGGCG+TGG	+	chr8_4:27654187-27654206	Msa1321590:intron	40.0%
	TTGACTCAGTGTGACAAATG+TGG	+	chr8_4:27654366-27654385	Msa1321590:intron	40.0%
	ACAAATGTGGTTACAGCTTC+TGG	+	chr8_4:27654379-27654398	Msa1321590:intron	40.0%
	ACAGCTTCTGGCAAATCAAT+TGG	+	chr8_4:27654391-27654410	Msa1321590:intron	40.0%
	CAATGCATCTTCTGCTTGTT+GGG	-	chr8_4:27654468-27654487	None:intergenic	40.0%
	ACAATGCATCTTCTGCTTGT+TGG	-	chr8_4:27654469-27654488	None:intergenic	40.0%
	GGGAATGTGGCAAATTACAT+GGG	+	chr8_4:27654566-27654585	Msa1321590:CDS	40.0%
	GGCAAATTACATGGGTCAAA+TGG	+	chr8_4:27654574-27654593	Msa1321590:CDS	40.0%
	TTACATGGGTCAAATGGCTA+TGG	+	chr8_4:27654580-27654599	Msa1321590:CDS	40.0%
!!	CCATCCATGATTACTTTTCG+CGG	+	chr8_4:27654957-27654976	Msa1321590:CDS	40.0%
	TCCTAGAGTTCACATAGTCT+AGG	+	chr8_4:27655164-27655183	Msa1321590:three_prime_UTR	40.0%
	TCCTAGACTATGTGAACTCT+AGG	-	chr8_4:27655168-27655187	None:intergenic	40.0%
	GAGTTCACATAGTCTAGGAA+TGG	+	chr8_4:27655169-27655188	Msa1321590:three_prime_UTR	40.0%
	ACATCTGGGTCTAACTGAGT+TGG	+	chr8_4:27648860-27648879	Msa1321590:intron	45.0%
!!	GTTTGGTTTGTGGGTACACA+TGG	+	chr8_4:27648968-27648987	Msa1321590:intron	45.0%
	ATGAGAAGGGAGCAGAAAGA+GGG	+	chr8_4:27649448-27649467	Msa1321590:CDS	45.0%
	CAAAACCGCTCTGCCTTATA+TGG	+	chr8_4:27649820-27649839	Msa1321590:intron	45.0%
	TTGGTCCATATAAGGCAGAG+CGG	-	chr8_4:27649828-27649847	None:intergenic	45.0%
	TCTGCCTTATATGGACCAAC+TGG	+	chr8_4:27649829-27649848	Msa1321590:intron	45.0%
	CTGTGAATATCTTCCTTGGG+TGG	+	chr8_4:27649941-27649960	Msa1321590:intron	45.0%
	AGGTTTAACCTAACCACCCA+AGG	-	chr8_4:27649957-27649976	None:intergenic	45.0%
	TCATGGTGTATCGTGTTCAC+TGG	+	chr8_4:27650258-27650277	Msa1321590:intron	45.0%
	CATGGTGTATCGTGTTCACT+GGG	+	chr8_4:27650259-27650278	Msa1321590:intron	45.0%
	ACAGCATCCTCTAAGCGATA+TGG	-	chr8_4:27650485-27650504	None:intergenic	45.0%
	AGTGGCCAAACAATTTCTCC+TGG	+	chr8_4:27651138-27651157	Msa1321590:CDS	45.0%
!	GCTCTGTTTGGTTCGTCAAT+GGG	-	chr8_4:27651326-27651345	None:intergenic	45.0%
	CTTAATATGGTACCGCTACC+TGG	-	chr8_4:27651387-27651406	None:intergenic	45.0%
	CGAAATATCAGTCCTAGGAC+TGG	-	chr8_4:27651451-27651470	None:intergenic	45.0%
	ACACCGATGACAAGAATCAG+CGG	+	chr8_4:27651481-27651500	Msa1321590:CDS	45.0%
	AGATGTGCAAATGCAGGACA+GGG	-	chr8_4:27651566-27651585	None:intergenic	45.0%
	GTTTCCAGATGTGCAAATGC+AGG	-	chr8_4:27651572-27651591	None:intergenic	45.0%
	TGACTCCAGTGACAGATCAA+AGG	+	chr8_4:27652001-27652020	Msa1321590:CDS	45.0%
	GCCGGTTGAGAAAGAAAGTA+AGG	+	chr8_4:27652183-27652202	Msa1321590:intron	45.0%
	CTGAAGCTCTTGCTCTAGTT+GGG	-	chr8_4:27653011-27653030	None:intergenic	45.0%
	ACTGAATCTAACCTGTTGCC+TGG	-	chr8_4:27653038-27653057	None:intergenic	45.0%
	AGAAAGAGAGAGCAGACAAC+TGG	-	chr8_4:27653083-27653102	None:intergenic	45.0%
	GGGAGTCTTCATATCAAGCT+CGG	+	chr8_4:27653186-27653205	Msa1321590:intron	45.0%
	GGAGTCTTCATATCAAGCTC+GGG	+	chr8_4:27653187-27653206	Msa1321590:CDS	45.0%
!	TGGTGTTTTACGCTGATGGT+GGG	-	chr8_4:27653669-27653688	None:intergenic	45.0%
!	ATGGTGTTTTACGCTGATGG+TGG	-	chr8_4:27653670-27653689	None:intergenic	45.0%
	TCGATGCAGAATATGCAAGG+TGG	+	chr8_4:27653788-27653807	Msa1321590:CDS	45.0%
!!	TCCATTTGTTGTCTGGCATG+TGG	+	chr8_4:27653962-27653981	Msa1321590:CDS	45.0%
	TCCACATGCCAGACAACAAA+TGG	-	chr8_4:27653966-27653985	None:intergenic	45.0%
	TGTGGAAAACACCTGCTGAA+AGG	+	chr8_4:27653980-27653999	Msa1321590:CDS	45.0%
!	AGGTGTTTCTTATGGCTTGG+TGG	+	chr8_4:27654000-27654019	Msa1321590:CDS	45.0%
	TCGGTCATCTGAACTTCTCA+AGG	+	chr8_4:27654026-27654045	Msa1321590:CDS	45.0%
	CATCTGAACTTCTCAAGGTG+AGG	+	chr8_4:27654031-27654050	Msa1321590:intron	45.0%
	TTGGGAGGATTGTTGCAAGT+TGG	-	chr8_4:27654450-27654469	None:intergenic	45.0%
	AGCAGAAGATGCATTGTCTC+AGG	+	chr8_4:27654472-27654491	Msa1321590:CDS	45.0%
	GCAGAAGATGCATTGTCTCA+GGG	+	chr8_4:27654473-27654492	Msa1321590:CDS	45.0%
	AGATGCATTGTCTCAGGGTA+TGG	+	chr8_4:27654478-27654497	Msa1321590:CDS	45.0%
	GGGGAATGTGGCAAATTACA+TGG	+	chr8_4:27654565-27654584	Msa1321590:CDS	45.0%
	GAGTGTTCCAAGTTTACCCA+TGG	-	chr8_4:27654606-27654625	None:intergenic	45.0%
	GGTAAACTTGGAACACTCGA+GGG	+	chr8_4:27654608-27654627	Msa1321590:CDS	45.0%
!!	CTATGTAGCACCGACACTTT+AGG	+	chr8_4:27654728-27654747	Msa1321590:intron	45.0%
!!	GCATTTGTTGTAGTGTCTGC+TGG	-	chr8_4:27654895-27654914	None:intergenic	45.0%
	CCGCGAAAAGTAATCATGGA+TGG	-	chr8_4:27654960-27654979	None:intergenic	45.0%
!!	AAATTTAAAGATAATAAATA+TGG	-	chr8_4:27652723-27652742	None:intergenic	5.0%
!!	TGATGGTGGGAGTGTTGCAT+GGG	+	chr8_4:27649399-27649418	Msa1321590:exon	50.0%
	GATGAGAAGGGAGCAGAAAG+AGG	+	chr8_4:27649447-27649466	Msa1321590:CDS	50.0%
	CATGCATCGGAAGTGAAGCT+CGG	-	chr8_4:27649477-27649496	None:intergenic	50.0%
!	GCAGAGCGGTTTTGCATAGA+GGG	-	chr8_4:27649814-27649833	None:intergenic	50.0%
!!	GCTCTAGTCTTGGAGAGCAT+CGG	+	chr8_4:27650144-27650163	Msa1321590:intron	50.0%
	CAGGGCCAGGAGAAATTGTT+TGG	-	chr8_4:27651146-27651165	None:intergenic	50.0%
!	GGGCTTTTATCGCGTGATGT+TGG	-	chr8_4:27651306-27651325	None:intergenic	50.0%
!	GGCTCTGTTTGGTTCGTCAA+TGG	-	chr8_4:27651327-27651346	None:intergenic	50.0%
	CGAACCAAACAGAGCCACAT+AGG	+	chr8_4:27651331-27651350	Msa1321590:CDS	50.0%
!	GCAACCTATGTGGCTCTGTT+TGG	-	chr8_4:27651338-27651357	None:intergenic	50.0%
!	ATCAGTCCTAGGACTGGCAT+CGG	-	chr8_4:27651445-27651464	None:intergenic	50.0%
	CACCGATGACAAGAATCAGC+GGG	+	chr8_4:27651482-27651501	Msa1321590:CDS	50.0%
!!	CACCCGCTGATTCTTGTCAT+CGG	-	chr8_4:27651487-27651506	None:intergenic	50.0%
	CAGATGTGCAAATGCAGGAC+AGG	-	chr8_4:27651567-27651586	None:intergenic	50.0%
	CTGTCCTGCATTTGCACATC+TGG	+	chr8_4:27651565-27651584	Msa1321590:intron	50.0%
	GCTGAAGCTCTTGCTCTAGT+TGG	-	chr8_4:27653012-27653031	None:intergenic	50.0%
	CTTCGCAGCTGAGAGAAGAA+AGG	+	chr8_4:27653630-27653649	Msa1321590:intron	50.0%
	TGCAGAATATGCAAGGTGGC+TGG	+	chr8_4:27653792-27653811	Msa1321590:CDS	50.0%
!!	GCTGAAAGGTGTTGCAGCTA+AGG	+	chr8_4:27653930-27653949	Msa1321590:CDS	50.0%
	GAACTTTCTTTGGCGTGGCA+TGG	+	chr8_4:27654192-27654211	Msa1321590:intron	50.0%
	TTTCTTTGGCGTGGCATGGT+GGG	+	chr8_4:27654196-27654215	Msa1321590:intron	50.0%
	ATCAACTGCTGCTCAGTGAG+AGG	-	chr8_4:27654419-27654438	None:intergenic	50.0%
	TCTCACTGAGCAGCAGTTGA+TGG	+	chr8_4:27654418-27654437	Msa1321590:CDS	50.0%
	CTCACTGAGCAGCAGTTGAT+GGG	+	chr8_4:27654419-27654438	Msa1321590:CDS	50.0%
!	TGCATCTTCTGCTTGTTGGG+AGG	-	chr8_4:27654465-27654484	None:intergenic	50.0%
!	ACCTTCCTCATCTGGTTCCT+CGG	+	chr8_4:27654544-27654563	Msa1321590:CDS	50.0%
!	GTAATTTGCCACATTCCCCG+AGG	-	chr8_4:27654564-27654583	None:intergenic	50.0%
	GGTCAAATGGCTATGGCCAT+GGG	+	chr8_4:27654587-27654606	Msa1321590:CDS	50.0%
	GCTATGGCCATGGGTAAACT+TGG	+	chr8_4:27654596-27654615	Msa1321590:CDS	50.0%
	GGGTAAACTTGGAACACTCG+AGG	+	chr8_4:27654607-27654626	Msa1321590:CDS	50.0%
	GCAGCCGCGAAAAGTAATCA+TGG	-	chr8_4:27654964-27654983	None:intergenic	50.0%
!!	GTGATGGTGGGAGTGTTGCA+TGG	+	chr8_4:27649398-27649417	Msa1321590:exon	55.0%
	CGAGCTTCACTTCCGATGCA+TGG	+	chr8_4:27649475-27649494	Msa1321590:CDS	55.0%
!	GGCAGAGCGGTTTTGCATAG+AGG	-	chr8_4:27649815-27649834	None:intergenic	55.0%
!	GAGGATGCTGTTGCTCTGAG+TGG	+	chr8_4:27650494-27650513	Msa1321590:CDS	55.0%
	GAACTGGTATGAACAGGGCC+AGG	-	chr8_4:27651159-27651178	None:intergenic	55.0%
	CGGCCATGGCAAATTCTTCC+TGG	-	chr8_4:27651425-27651444	None:intergenic	55.0%
	GCATAAGCTACGGCCCTGTT+TGG	-	chr8_4:27651810-27651829	None:intergenic	55.0%
	GTGAGGCGGCAAGAAAAAGC+CGG	+	chr8_4:27652165-27652184	Msa1321590:CDS	55.0%
	CTTTCTTTGGCGTGGCATGG+TGG	+	chr8_4:27654195-27654214	Msa1321590:intron	55.0%
	AGTGGACAGCGTCTCTGCAA+GGG	-	chr8_4:27654519-27654538	None:intergenic	55.0%
!	AGATGAGGAAGGTGCACCAG+TGG	-	chr8_4:27654537-27654556	None:intergenic	55.0%
	ACTGGTGCACCTTCCTCATC+TGG	+	chr8_4:27654536-27654555	Msa1321590:CDS	55.0%
!	CCTTCCTCATCTGGTTCCTC+GGG	+	chr8_4:27654545-27654564	Msa1321590:CDS	55.0%
!	CTTCCTCATCTGGTTCCTCG+GGG	+	chr8_4:27654546-27654565	Msa1321590:CDS	55.0%
	ATTCCCCGAGGAACCAGATG+AGG	-	chr8_4:27654552-27654571	None:intergenic	55.0%
!	ATCTGGTTCCTCGGGGAATG+TGG	+	chr8_4:27654553-27654572	Msa1321590:CDS	55.0%
	GGGTCAAATGGCTATGGCCA+TGG	+	chr8_4:27654586-27654605	Msa1321590:CDS	55.0%
	ACTCGAGGGCTTCATTCGAC+AGG	+	chr8_4:27654622-27654641	Msa1321590:CDS	55.0%
!!	TCTCTGGTTGGCTCGTCCAA+GGG	+	chr8_4:27654997-27655016	Msa1321590:CDS	55.0%
	CACGATTCTGAGCGAGCCTG+CGG	-	chr8_4:27652148-27652167	None:intergenic	60.0%
	CAGGCTCGCTCAGAATCGTG+AGG	+	chr8_4:27652148-27652167	Msa1321590:CDS	60.0%
	GCTCGCTCAGAATCGTGAGG+CGG	+	chr8_4:27652151-27652170	Msa1321590:CDS	60.0%
	AGCAAGAGCTTCAGCGAGCC+AGG	+	chr8_4:27653017-27653036	Msa1321590:CDS	60.0%
	CAGTGGACAGCGTCTCTGCA+AGG	-	chr8_4:27654520-27654539	None:intergenic	60.0%
	CTTGCAGAGACGCTGTCCAC+TGG	+	chr8_4:27654518-27654537	Msa1321590:CDS	60.0%
	CCCGAGGAACCAGATGAGGA+AGG	-	chr8_4:27654548-27654567	None:intergenic	60.0%
	CGCTCTCAGCTCTCTCTGGT+TGG	+	chr8_4:27654985-27655004	Msa1321590:CDS	60.0%
!!	CTCTCTGGTTGGCTCGTCCA+AGG	+	chr8_4:27654996-27655015	Msa1321590:CDS	60.0%
!!	CCTAGGACTGGCATCGGCCA+TGG	-	chr8_4:27651439-27651458	None:intergenic	65.0%
	CCATGGCCGATGCCAGTCCT+AGG	+	chr8_4:27651436-27651455	Msa1321590:CDS	65.0%
	GCTTCAGCGAGCCAGGCAAC+AGG	+	chr8_4:27653024-27653043	Msa1321590:CDS	65.0%
	TGCGCGCTCTCAGCTCTCTC+TGG	+	chr8_4:27654981-27655000	Msa1321590:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	27648464	27655341	27648464	ID=Msa1321590;Name=Msa1321590
chr8_4	mRNA	27648464	27655341	27648464	ID=Msa1321590-mRNA-1;Parent=Msa1321590;Name=Msa1321590-mRNA-1;_AED=0.12;_eAED=0.12;_QI=248\|1\|1\|1\|0.90\|0.83\|12\|317\|465
chr8_4	exon	27648464	27648644	27648464	ID=Msa1321590-mRNA-1:exon:9241;Parent=Msa1321590-mRNA-1
chr8_4	exon	27649350	27649496	27649350	ID=Msa1321590-mRNA-1:exon:9242;Parent=Msa1321590-mRNA-1
chr8_4	exon	27650421	27650518	27650421	ID=Msa1321590-mRNA-1:exon:9243;Parent=Msa1321590-mRNA-1
chr8_4	exon	27651106	27651188	27651106	ID=Msa1321590-mRNA-1:exon:9244;Parent=Msa1321590-mRNA-1
chr8_4	exon	27651300	27651503	27651300	ID=Msa1321590-mRNA-1:exon:9245;Parent=Msa1321590-mRNA-1
chr8_4	exon	27651966	27652040	27651966	ID=Msa1321590-mRNA-1:exon:9246;Parent=Msa1321590-mRNA-1
chr8_4	exon	27652140	27652199	27652140	ID=Msa1321590-mRNA-1:exon:9247;Parent=Msa1321590-mRNA-1
chr8_4	exon	27652968	27653045	27652968	ID=Msa1321590-mRNA-1:exon:9248;Parent=Msa1321590-mRNA-1
chr8_4	exon	27653187	27653238	27653187	ID=Msa1321590-mRNA-1:exon:9249;Parent=Msa1321590-mRNA-1
chr8_4	exon	27653776	27654047	27653776	ID=Msa1321590-mRNA-1:exon:9250;Parent=Msa1321590-mRNA-1
chr8_4	exon	27654395	27654643	27654395	ID=Msa1321590-mRNA-1:exon:9251;Parent=Msa1321590-mRNA-1
chr8_4	exon	27654878	27655341	27654878	ID=Msa1321590-mRNA-1:exon:9252;Parent=Msa1321590-mRNA-1
chr8_4	five_prime_UTR	27648464	27648644	27648464	ID=Msa1321590-mRNA-1:five_prime_utr;Parent=Msa1321590-mRNA-1
chr8_4	five_prime_UTR	27649350	27649416	27649350	ID=Msa1321590-mRNA-1:five_prime_utr;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27649417	27649496	27649417	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27650421	27650518	27650421	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27651106	27651188	27651106	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27651300	27651503	27651300	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27651966	27652040	27651966	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27652140	27652199	27652140	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27652968	27653045	27652968	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27653187	27653238	27653187	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27653776	27654047	27653776	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27654395	27654643	27654395	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	CDS	27654878	27655024	27654878	ID=Msa1321590-mRNA-1:cds;Parent=Msa1321590-mRNA-1
chr8_4	three_prime_UTR	27655025	27655341	27655025	ID=Msa1321590-mRNA-1:three_prime_utr;Parent=Msa1321590-mRNA-1

Gene Sequence

>Msa1321590

ATGGGAAGTAGAATCAGAGTTGGTGTTGAAGATGAGAAGGGAGCAGAAAGAGGGATGCCGAGCTTCACTTCCGATGCATGCTATACAGATGGGAACACAATTGATAATTTTCATGTTTCTGACTTTGGAGCATTTAGTCAACCATATCGCTTAGAGGATGCTGTTGCTCTGAGTGGAAATTCAAACTCGGTTTTTAACTCACTTAAAGTAAGTGGCCAAACAATTTCTCCTGGCCCTGTTCATACCAGTTCTCTTGATAAGTTGCCAACATCACGCGATAAAAGCCCATTGACGAACCAAACAGAGCCACATAGGTTGCAATTAACTAAGCTTCAATCATCAAATCCAGGTAGCGGTACCATATTAAGTGTTCATACAGAAAACCAGGAAGAATTTGCCATGGCCGATGCCAGTCCTAGGACTGATATTTCGACAGATGGAGACACCGATGACAAGAATCAGCGGTTTGATAGAAATCAATCACTTGCTGTTGCGAATTCTGACTCCAGTGACAGATCAAAGGATAAATCAGATCAGAAGACTCTCCGCAGGCTCGCTCAGAATCGTGAGGCGGCAAGAAAAAGCCGGTTGAGAAAGAAAGCCTATGTCCAACAATTGGAAAGTAGTCGTTTGAAGCTGACCCAACTAGAGCAAGAGCTTCAGCGAGCCAGGCAACAGGGAGTCTTCATATCAAGCTCGGGTGAACAAACACATTCACTGAGTGGAAATGGAGCAATGCAATTCGATGCAGAATATGCAAGGTGGCTGGAAGAGCAGAATCGACAAATTAATGAGCTGAGAGCAGCTGTAAATTCTCATGCAAGTGATACCGAACTTCGCATGATTGTTGATGGTGTAGTGGCACATTATGATGAGATTTTTAGGCTGAAAGGTGTTGCAGCTAAGGCTGATGTTTTCCATTTGTTGTCTGGCATGTGGAAAACACCTGCTGAAAGGTGTTTCTTATGGCTTGGTGGTTTTCGGTCATCTGAACTTCTCAAGCTTCTGGCAAATCAATTGGAACCTCTCACTGAGCAGCAGTTGATGGGTATTACCAACTTGCAACAATCCTCCCAACAAGCAGAAGATGCATTGTCTCAGGGTATGGAAGCATTACAACAGTCCCTTGCAGAGACGCTGTCCACTGGTGCACCTTCCTCATCTGGTTCCTCGGGGAATGTGGCAAATTACATGGGTCAAATGGCTATGGCCATGGGTAAACTTGGAACACTCGAGGGCTTCATTCGACAGGCTGACAATCTGCGCCAGCAGACACTACAACAAATGCACCGAATATTGACAACACGACAATCGGCTCGTGCACTCCTTGCCATCCATGATTACTTTTCGCGGCTGCGCGCTCTCAGCTCTCTCTGGTTGGCTCGTCCAAGGGATTAA

Protein sequence

>Msa1321590

MGSRIRVGVEDEKGAERGMPSFTSDACYTDGNTIDNFHVSDFGAFSQPYRLEDAVALSGNSNSVFNSLKVSGQTISPGPVHTSSLDKLPTSRDKSPLTNQTEPHRLQLTKLQSSNPGSGTILSVHTENQEEFAMADASPRTDISTDGDTDDKNQRFDRNQSLAVANSDSSDRSKDKSDQKTLRRLAQNREAARKSRLRKKAYVQQLESSRLKLTQLEQELQRARQQGVFISSSGEQTHSLSGNGAMQFDAEYARWLEEQNRQINELRAAVNSHASDTELRMIVDGVVAHYDEIFRLKGVAAKADVFHLLSGMWKTPAERCFLWLGGFRSSELLKLLANQLEPLTEQQLMGITNLQQSSQQAEDALSQGMEALQQSLAETLSTGAPSSSGSSGNVANYMGQMAMAMGKLGTLEGFIRQADNLRQQTLQQMHRILTTRQSARALLAIHDYFSRLRALSSLWLARPRD*