AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1325820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1325820 MtrunA17_Chr8g0360371 87.662 308 33 3 1 308 1 303 2.81e-147 416
Msa1325820 MtrunA17_Chr2g0317231 68.033 122 38 1 1 122 3 123 8.50e-63 213
Msa1325820 MtrunA17_Chr2g0324711 42.561 289 145 8 7 276 8 294 3.48e-55 183
Msa1325820 MtrunA17_Chr2g0324721 46.927 179 86 3 1 173 4 179 9.27e-48 159
Msa1325820 MtrunA17_Chr8g0385031 38.832 291 144 9 1 272 1 276 5.64e-45 154
Msa1325820 MtrunA17_Chr8g0385021 39.130 253 130 5 1 232 1 250 7.79e-43 148
Msa1325820 MtrunA17_Chr8g0385041 40.639 219 101 7 22 231 23 221 1.15e-39 141
Msa1325820 MtrunA17_Chr4g0040291 51.887 106 51 0 20 125 21 126 3.16e-39 136
Msa1325820 MtrunA17_Chr4g0041811 46.281 121 63 2 7 125 15 135 7.31e-36 129
Msa1325820 MtrunA17_Chr3g0136871 45.833 144 66 4 21 161 24 158 1.44e-35 126
Msa1325820 MtrunA17_Chr1g0150131 45.455 121 63 2 8 125 9 129 8.93e-35 124
Msa1325820 MtrunA17_Chr3g0127521 34.317 271 152 5 1 245 2 272 3.24e-33 124
Msa1325820 MtrunA17_Chr2g0331181 48.000 125 57 3 12 133 18 137 1.01e-31 117
Msa1325820 MtrunA17_Chr2g0319611 43.478 115 61 3 21 131 23 137 2.07e-30 115
Msa1325820 MtrunA17_Chr6g0458331 49.541 109 50 2 26 131 39 145 1.72e-29 110
Msa1325820 MtrunA17_Chr4g0039721 38.356 146 75 5 5 142 12 150 1.57e-27 105
Msa1325820 MtrunA17_Chr4g0062341 36.970 165 86 5 9 163 12 168 1.09e-26 103
Msa1325820 MtrunA17_Chr4g0062311 36.970 165 86 5 9 163 12 168 1.09e-26 103
Msa1325820 MtrunA17_Chr7g0256441 38.889 144 59 5 21 161 24 141 4.93e-24 95.5
Msa1325820 MtrunA17_Chr5g0394321 31.410 156 105 1 7 162 9 162 1.94e-22 92.0
Msa1325820 MtrunA17_Chr4g0073901 34.167 120 74 4 7 121 10 129 8.55e-20 85.1
Msa1325820 MtrunA17_Chr2g0323021 32.031 128 83 3 4 130 5 129 3.32e-18 82.0
Msa1325820 MtrunA17_Chr2g0289741 34.959 123 71 4 11 132 15 129 1.15e-17 79.0
Msa1325820 MtrunA17_Chr8g0379081 49.275 69 33 1 69 135 2 70 3.42e-17 76.3
Msa1325820 MtrunA17_Chr4g0073921 34.146 123 77 4 5 123 9 131 5.20e-17 79.3
Msa1325820 MtrunA17_Chr4g0064041 37.000 100 61 1 24 121 191 290 7.11e-17 80.1
Msa1325820 MtrunA17_Chr4g0064041 31.111 135 84 2 10 135 8 142 2.89e-16 78.6
Msa1325820 MtrunA17_Chr2g0306461 35.043 117 73 3 7 121 10 125 1.53e-16 76.6
Msa1325820 MtrunA17_Chr5g0440711 34.307 137 83 5 4 137 9 141 2.96e-16 74.3
Msa1325820 MtrunA17_Chr1g0188981 31.731 104 68 1 21 121 26 129 9.06e-16 74.3
Msa1325820 MtrunA17_Chr1g0205701 31.250 112 77 0 11 122 12 123 1.56e-15 73.6
Msa1325820 MtrunA17_Chr8g0387804 32.258 124 78 2 4 121 4 127 2.06e-15 71.6
Msa1325820 MtrunA17_Chr3g0132431 34.752 141 85 4 1 138 1 137 3.68e-15 72.4
Msa1325820 MtrunA17_Chr4g0064031 36.290 124 73 4 16 134 20 142 9.69e-15 70.9
Msa1325820 MtrunA17_Chr4g0033131 31.387 137 81 5 7 137 10 139 1.28e-14 70.5
Msa1325820 MtrunA17_Chr4g0033531 31.387 137 81 5 7 137 10 139 1.28e-14 70.5
Msa1325820 MtrunA17_Chr1g0196801 33.793 145 86 4 18 155 17 158 1.85e-14 70.5
Msa1325820 MtrunA17_Chr6g0453921 36.232 138 79 5 1 135 17 148 2.65e-14 70.1
Msa1325820 MtrunA17_Chr8g0335531 37.736 159 81 8 5 153 6 156 4.79e-14 69.3
Msa1325820 MtrunA17_Chr1g0211101 27.632 152 101 4 3 150 12 158 9.25e-14 68.6
Msa1325820 MtrunA17_Chr3g0132441 37.097 124 71 4 1 121 1 120 5.71e-13 66.2
Msa1325820 MtrunA17_Chr8g0335571 35.833 120 71 4 5 122 6 121 6.96e-13 66.2
Msa1325820 MtrunA17_Chr6g0453801 36.667 120 70 4 5 121 15 131 7.96e-13 65.9
Msa1325820 MtrunA17_Chr7g0253641 31.667 120 76 3 5 122 7 122 7.85e-12 63.9
Msa1325820 MtrunA17_Chr6g0481491 31.933 119 74 3 3 121 11 122 9.27e-12 63.5
Msa1325820 MtrunA17_Chr3g0132511 35.000 100 62 2 23 121 25 122 2.49e-11 62.4
Msa1325820 MtrunA17_Chr6g0459441 34.021 97 60 3 26 121 28 121 4.71e-11 60.8
Msa1325820 MtrunA17_Chr6g0483981 33.140 172 91 6 7 172 8 161 5.94e-11 63.2
Msa1325820 MtrunA17_Chr3g0132411 32.456 114 73 3 4 117 46 155 9.22e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1325820 AT4G27520.1 54.198 131 56 1 1 127 4 134 9.15e-50 169
Msa1325820 AT5G53870.1 41.418 268 142 7 8 273 12 266 4.47e-49 167
Msa1325820 AT5G53870.2 47.917 192 82 5 8 197 12 187 4.62e-49 167
Msa1325820 AT4G28365.1 48.366 153 72 3 1 146 5 157 1.81e-47 158
Msa1325820 AT4G32490.1 50.926 108 53 0 16 123 23 130 3.57e-41 142
Msa1325820 AT2G25060.1 43.678 174 76 5 4 174 13 167 1.72e-38 134
Msa1325820 AT4G31840.1 46.259 147 75 3 5 148 8 153 1.37e-36 129
Msa1325820 AT5G57920.1 44.954 109 57 2 19 124 18 126 1.90e-32 119
Msa1325820 AT5G57920.2 41.667 120 67 2 8 124 6 125 2.48e-32 118
Msa1325820 AT5G57920.3 42.188 128 69 3 19 141 50 177 7.45e-32 118
Msa1325820 AT4G30590.1 38.922 167 79 4 1 160 1 151 7.71e-32 117
Msa1325820 AT5G25090.1 43.802 121 65 2 8 125 10 130 1.85e-31 116
Msa1325820 AT3G20570.1 37.143 175 98 3 9 182 15 178 1.55e-30 114
Msa1325820 AT1G48940.1 45.902 122 56 3 5 122 11 126 2.37e-27 105
Msa1325820 AT2G23990.1 39.744 156 79 5 5 156 8 152 9.78e-27 104
Msa1325820 AT1G79800.1 39.073 151 74 5 4 146 11 151 4.81e-26 102
Msa1325820 AT5G14345.1 44.545 110 55 3 28 134 20 126 2.33e-25 99.4
Msa1325820 AT3G18590.1 45.082 122 60 3 5 122 9 127 3.03e-25 100
Msa1325820 AT2G23990.2 36.000 175 78 6 5 156 8 171 3.25e-22 93.2
Msa1325820 AT2G31050.1 39.450 109 65 1 26 134 31 138 3.57e-20 87.0
Msa1325820 AT5G26330.1 34.513 113 74 0 12 124 12 124 3.40e-18 81.3
Msa1325820 AT2G26720.1 37.113 97 60 1 26 122 31 126 6.44e-17 78.2
Msa1325820 AT5G07475.1 31.507 146 93 4 1 143 7 148 9.39e-17 77.4
Msa1325820 AT2G32300.1 30.128 156 92 3 10 165 12 150 2.86e-16 77.4
Msa1325820 AT2G32300.2 30.128 156 92 3 10 165 42 180 4.49e-15 74.7
Msa1325820 AT3G17675.1 30.252 119 79 2 5 121 11 127 7.32e-15 70.5
Msa1325820 AT3G60270.1 36.893 103 60 3 21 122 23 121 3.68e-14 70.1
Msa1325820 AT5G15350.1 35.000 120 73 3 23 140 28 144 8.88e-14 68.6
Msa1325820 AT1G64640.1 31.933 119 79 2 24 141 33 150 3.85e-13 67.4
Msa1325820 AT4G12880.1 34.653 101 61 3 23 121 28 125 3.75e-12 63.5
Msa1325820 AT2G27035.1 30.070 143 84 5 10 151 15 142 6.51e-12 63.2
Msa1325820 AT5G20230.1 32.353 102 66 2 23 122 24 124 9.45e-12 63.5
Msa1325820 AT1G72230.1 33.613 119 68 3 3 121 10 117 1.17e-11 62.8
Msa1325820 AT3G27200.1 29.600 125 82 4 1 122 1 122 2.48e-11 62.0

Find 136 sgRNAs with CRISPR-Local

Find 348 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAGCTCCTTCGAGCTTTC+TGG 0.120831 8_4:-38490134 Msa1325820:CDS
CGCTGGAGCTGCTGCTCCCC+CGG 0.274437 8_4:+38490207 None:intergenic
CTTGGTGAAGGAGATGATTT+TGG 0.279381 8_4:+38490610 None:intergenic
GCTGGAACAGGGGAGCTTGC+TGG 0.296743 8_4:+38490499 None:intergenic
CCATCTTCGAATTTCTTGAT+TGG 0.318575 8_4:+38490748 None:intergenic
TACCTATAGTGTCACTGATT+TGG 0.332683 8_4:+38491386 None:intergenic
CATCTTCGAATTTCTTGATT+GGG 0.341423 8_4:+38490749 None:intergenic
GGTCCTCCGGCACCAGGACC+CGG 0.348865 8_4:-38490286 Msa1325820:CDS
GTAGGTCCACCGGCTGGAAC+AGG 0.370740 8_4:+38490487 None:intergenic
CCATCAGGGGCACCAGGACC+CGG 0.380832 8_4:-38490250 Msa1325820:CDS
TCCTGGTGCAGCTGATCCCC+CGG 0.383431 8_4:+38490231 None:intergenic
GGATCAGCTGCACCAGGACC+CGG 0.386077 8_4:-38490226 Msa1325820:CDS
TGATGGAGTCGCTGCTCCCC+CGG 0.392645 8_4:+38490267 None:intergenic
ACCTGTTCCTTCCAGCCCAC+TGG 0.419128 8_4:+38491410 None:intergenic
GGAGGACCACCTGGTGTAGA+TGG 0.422147 8_4:+38490301 None:intergenic
TCCACCAACGAAATACTTGA+AGG 0.422626 8_4:+38491471 None:intergenic
CCGGGTCCTGGTGCCCCTGA+TGG 0.429925 8_4:+38490250 None:intergenic
ATCTAATTCAACTGCACCAC+CGG 0.438406 8_4:-38490173 Msa1325820:CDS
AAAAGATCAAAACTGTGAAA+AGG 0.439027 8_4:-38490674 Msa1325820:CDS
GTCCTCCGGCACCAGGACCC+GGG 0.445759 8_4:-38490285 Msa1325820:CDS
GATCAGCTGCACCAGGACCC+GGG 0.447402 8_4:-38490225 Msa1325820:CDS
CATCAGGGGCACCAGGACCC+GGG 0.448864 8_4:-38490249 Msa1325820:CDS
AGCACCAACGGCAAGGGTTG+CGG 0.449999 8_4:+38490105 None:intergenic
GCCTTCAAGTATTTCGTTGG+TGG 0.451428 8_4:-38491472 Msa1325820:CDS
CCACCTGCTGGTGAAGGAGC+TGG 0.452499 8_4:+38490325 None:intergenic
AAAGATCAAAACTGTGAAAA+GGG 0.454501 8_4:-38490673 Msa1325820:CDS
GAGAATTACAACCAGTGGGC+TGG 0.458809 8_4:-38491421 Msa1325820:CDS
GCTGGAGGTGTGCTCACTAC+TGG 0.459593 8_4:+38490391 None:intergenic
ACCTCCGGCTGGTGGACCTA+TGG 0.461437 8_4:-38490434 Msa1325820:CDS
TCTCCTTCACCAAGTGGTGG+TGG 0.464912 8_4:-38490601 Msa1325820:CDS
GCAGCTCCAGCGGGGTCACC+AGG 0.465912 8_4:-38490196 Msa1325820:CDS
GCCGGAGGTGTGCTCACTAC+TGG 0.466620 8_4:+38490448 None:intergenic
AGCTCCCCTGTTCCAGCCGG+TGG 0.468611 8_4:-38490493 Msa1325820:CDS
ACCAGTGGGCTGGAAGGAAC+AGG 0.473167 8_4:-38491411 Msa1325820:CDS
CCTGGTGCCGGAGGACCACC+TGG 0.478758 8_4:+38490292 None:intergenic
TTTCAAGTACAAGAAGGGTT+CGG 0.479224 8_4:-38490824 Msa1325820:CDS
CAAGTATTTCGTTGGTGGAA+AGG 0.487435 8_4:-38491467 Msa1325820:CDS
TTTCCACTGATGAAATAGAA+TGG 0.497104 8_4:+38490694 None:intergenic
ATCTACACCAGGTGGTCCTC+CGG 0.503454 8_4:-38490299 Msa1325820:CDS
CCAGGTGGTCCTCCGGCACC+AGG 0.503564 8_4:-38490292 Msa1325820:CDS
TGTTCCAGCCGGTGGACCTA+CGG 0.504775 8_4:-38490485 Msa1325820:CDS
GGAGCTGGTGGTCCACCTGC+CGG 0.504880 8_4:+38490340 None:intergenic
TATTTCGTTGGTGGAAAGGA+TGG 0.506955 8_4:-38491463 Msa1325820:CDS
GGTGGTCCACCTGCCGGAGA+AGG 0.507316 8_4:+38490346 None:intergenic
GAGTTCACTTTGGACAGAGC+AGG 0.508058 8_4:-38490718 Msa1325820:CDS
GCTTCATCTCCTTCTCCGGC+AGG 0.510071 8_4:-38490355 Msa1325820:CDS
TTAGATCCTGGTGACCCCGC+TGG 0.511126 8_4:+38490190 None:intergenic
ACCTCCAGCCGGTGGACCTA+CGG 0.511871 8_4:-38490377 Msa1325820:CDS
GCGACTCCATCAGGGGCACC+AGG 0.515771 8_4:-38490256 Msa1325820:CDS
CAAGCCTTCAAGTATTTCGT+TGG 0.517033 8_4:-38491475 Msa1325820:CDS
TCCTCCGGCACCAGGACCCG+GGG 0.517479 8_4:-38490284 Msa1325820:CDS
TATTCCGCAACCCTTGCCGT+TGG 0.520673 8_4:-38490109 Msa1325820:CDS
ATCAGCTGCACCAGGACCCG+GGG 0.524267 8_4:-38490224 Msa1325820:CDS
ATCAGGGGCACCAGGACCCG+GGG 0.524267 8_4:-38490248 Msa1325820:CDS
GTGAAGGATATTGAATTGAA+TGG 0.524878 8_4:+38491565 None:intergenic
GAAGGGTTCGGACTCGGTGT+TGG 0.525077 8_4:-38490812 Msa1325820:CDS
CCACCAGCTCCTTCACCAGC+AGG 0.525764 8_4:-38490328 Msa1325820:CDS
TTCCAAATCAGTGACACTAT+AGG 0.526349 8_4:-38491388 Msa1325820:intron
CACTGGTTGTAATTCTCAGA+AGG 0.527393 8_4:+38491427 None:intergenic
GTAATATGTGATGTGTTGTA+TGG 0.529396 8_4:+38491637 None:intergenic
CCAATCAAGAAATTCGAAGA+TGG 0.535933 8_4:-38490748 Msa1325820:CDS
GTACAAGAAGGGTTCGGACT+CGG 0.539622 8_4:-38490818 Msa1325820:CDS
GCAAGCTCCCCTGTTCCAGC+CGG 0.541586 8_4:-38490496 Msa1325820:CDS
GTGAAGAGAACAAAGAAGAG+AGG 0.543513 8_4:+38491517 None:intergenic
CTTGCTGGAGGAATAGCCGC+TGG 0.545733 8_4:+38490514 None:intergenic
TACGGCTTCATCTCCTTCTC+CGG 0.546654 8_4:-38490359 Msa1325820:CDS
AGATGAAGCCGTAGGTCCAC+CGG 0.546744 8_4:+38490369 None:intergenic
TGTGATGTGTTGTATGGTAA+AGG 0.547072 8_4:+38491643 None:intergenic
AGGTCCACCGGCTGGAACAG+GGG 0.547696 8_4:+38490489 None:intergenic
GAAGCCGTAGGTCCACCGGC+TGG 0.549612 8_4:+38490373 None:intergenic
CTCACTACTGGAGATGGAGA+TGG 0.551978 8_4:+38490403 None:intergenic
GTGAGCACACCTCCGGCTGG+TGG 0.552623 8_4:-38490442 Msa1325820:CDS
AGAAGATGCCGTAGGTCCAC+CGG 0.560317 8_4:+38490477 None:intergenic
GGGGAGCAGCGACTCCATCA+GGG 0.561209 8_4:-38490264 Msa1325820:CDS
TCCAGTAGTGAGCACACCTC+CGG 0.562951 8_4:-38490449 Msa1325820:CDS
GGTGTGCTCACTACTGGAGA+TGG 0.563208 8_4:+38490454 None:intergenic
TAGGTCCACCGGCTGGAACA+GGG 0.566664 8_4:+38490488 None:intergenic
TCATCTCCTTCTCCGGCAGG+TGG 0.568679 8_4:-38490352 Msa1325820:CDS
CTCCATAGCAAAATGTGTGA+AGG 0.568998 8_4:+38491549 None:intergenic
GTATGGTAAAGGAAATTGAA+TGG 0.573547 8_4:+38491654 None:intergenic
CCTGCTGGTGAAGGAGCTGG+TGG 0.574961 8_4:+38490328 None:intergenic
GGGCCATTCTATTTCATCAG+TGG 0.577208 8_4:-38490697 Msa1325820:CDS
AAACAGAGCACCAACGGCAA+GGG 0.581779 8_4:+38490099 None:intergenic
AGTTCACTTTGGACAGAGCA+GGG 0.582623 8_4:-38490717 Msa1325820:CDS
GATGCCGTAGGTCCACCGGC+TGG 0.582686 8_4:+38490481 None:intergenic
GGTGTGCTCACTACTGGAGA+TGG 0.585001 8_4:+38490397 None:intergenic
AAAACAGAGCACCAACGGCA+AGG 0.585538 8_4:+38490098 None:intergenic
GTAGTGAGCACACCTCCGGC+TGG 0.586869 8_4:-38490445 Msa1325820:CDS
GGAGGAATAGCCGCTGGAGA+TGG 0.587071 8_4:+38490520 None:intergenic
GTTGTCGTTGGTGACGGTGA+AGG 0.587432 8_4:+38490568 None:intergenic
GGGAGCAGCGACTCCATCAG+GGG 0.587521 8_4:-38490263 Msa1325820:CDS
GGTGGTGAAACAGAGGGTGA+TGG 0.589277 8_4:+38490541 None:intergenic
ACTGGTTGTAATTCTCAGAA+GGG 0.589662 8_4:+38491428 None:intergenic
GGTGTAGATGGACCACCTGC+TGG 0.589795 8_4:+38490313 None:intergenic
GGTGATGGAGTGGTTGTCGT+TGG 0.590401 8_4:+38490556 None:intergenic
TCGTTGGTGGAAAGGATGGA+TGG 0.594920 8_4:-38491459 Msa1325820:CDS
GGTGATAAGCCACCACCACT+TGG 0.595997 8_4:+38490592 None:intergenic
GGAGTGGTTGTCGTTGGTGA+CGG 0.597376 8_4:+38490562 None:intergenic
GCCGTAGGTCCACCGGCTGG+AGG 0.602135 8_4:+38490376 None:intergenic
ACAAATGAACCACTGCTACC+CGG 0.604696 8_4:+38490154 None:intergenic
GTAGTGAGCACACCTCCAGC+CGG 0.608290 8_4:-38490388 Msa1325820:CDS
ATTACAACCAGTGGGCTGGA+AGG 0.611232 8_4:-38491417 Msa1325820:CDS
TGGTGATACAGAGTTCACTT+TGG 0.612356 8_4:-38490728 Msa1325820:CDS
GTCGTTGGTGACGGTGAAGG+TGG 0.613000 8_4:+38490571 None:intergenic
GTGAGCACACCTCCAGCCGG+TGG 0.614961 8_4:-38490385 Msa1325820:CDS
GGAGATGGAGATGATGCCAT+AGG 0.617212 8_4:+38490418 None:intergenic
AAGCCACCACCACTTGGTGA+AGG 0.618943 8_4:+38490598 None:intergenic
TCTAATTCAACTGCACCACC+GGG 0.619239 8_4:-38490172 Msa1325820:CDS
TTCTGAGAATTACAACCAGT+GGG 0.619799 8_4:-38491425 Msa1325820:CDS
GCCATAGGTCCACCAGCCGG+AGG 0.620945 8_4:+38490433 None:intergenic
GGAACAGGGGAGCTTGCTGG+AGG 0.624417 8_4:+38490502 None:intergenic
GGTGCAGTTGAATTAGATCC+TGG 0.624618 8_4:+38490178 None:intergenic
ACTGCACCACCGGGTAGCAG+TGG 0.628398 8_4:-38490163 Msa1325820:CDS
TTCGTGGAGTTATAACAACA+AGG 0.629941 8_4:+38490638 None:intergenic
GATGCCATAGGTCCACCAGC+CGG 0.631786 8_4:+38490430 None:intergenic
CTTCTGAGAATTACAACCAG+TGG 0.634874 8_4:-38491426 Msa1325820:CDS
GATGGACCACCTGCTGGTGA+AGG 0.637837 8_4:+38490319 None:intergenic
CCAGCTCCTTCACCAGCAGG+TGG 0.640600 8_4:-38490325 Msa1325820:CDS
TGAAACAGAGGGTGATGGAG+TGG 0.649812 8_4:+38490546 None:intergenic
GAATACACCAGAAAGCTCGA+AGG 0.652876 8_4:+38490127 None:intergenic
GGAATAGCCGCTGGAGATGG+TGG 0.653879 8_4:+38490523 None:intergenic
TCATCTCCTTCACCAAGTGG+TGG 0.662715 8_4:-38490604 Msa1325820:CDS
TCAGCTGCACCAGGACCCGG+GGG 0.663432 8_4:-38490223 Msa1325820:CDS
AAATCATCTCCTTCACCAAG+TGG 0.664193 8_4:-38490607 Msa1325820:CDS
CCTCCGGCACCAGGACCCGG+GGG 0.666079 8_4:-38490283 Msa1325820:CDS
TCAGGGGCACCAGGACCCGG+GGG 0.668002 8_4:-38490247 Msa1325820:CDS
GGGGAGCAGCAGCTCCAGCG+GGG 0.672067 8_4:-38490204 Msa1325820:CDS
GGAGAAGGAGATGAAGCCGT+AGG 0.672907 8_4:+38490361 None:intergenic
TCGTGGAGTTATAACAACAA+GGG 0.673218 8_4:+38490639 None:intergenic
GCAGGTGGTCCATCTACACC+AGG 0.676664 8_4:-38490310 Msa1325820:CDS
GGAGATGGAGAAGATGCCGT+AGG 0.676847 8_4:+38490469 None:intergenic
TGGAGATGGTGGTGAAACAG+AGG 0.697284 8_4:+38490534 None:intergenic
GGAGATGGTGGTGAAACAGA+GGG 0.709029 8_4:+38490535 None:intergenic
AATGAACCACTGCTACCCGG+TGG 0.713699 8_4:+38490157 None:intergenic
TGTTTCACCACCATCTCCAG+CGG 0.715569 8_4:-38490530 Msa1325820:CDS
GGTGGTCCATCTACACCAGG+TGG 0.729161 8_4:-38490307 Msa1325820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATTAATATTAACATG+GGG + chr8_4:38490506-38490525 None:intergenic 10.0%
!! ATTAAATTAATATTAACATG+GGG + chr8_4:38490506-38490525 None:intergenic 10.0%
!! AAATCACAATAATTTCTTTA+TGG - chr8_4:38490776-38490795 Msa1325820:CDS 15.0%
!!! TATGATCCTTTAATTTTTTA+TGG - chr8_4:38490853-38490872 Msa1325820:intron 15.0%
!!! AAAATTTTATAGGAAAAACA+AGG + chr8_4:38490908-38490927 None:intergenic 15.0%
!!! AAAATTTTATAGGAAAAACA+AGG + chr8_4:38490908-38490927 None:intergenic 15.0%
!!! TTTAAGTTGCAAAATTTTAT+AGG + chr8_4:38490965-38490984 None:intergenic 15.0%
!! AAATCACAATAATTTCTTTA+TGG - chr8_4:38490776-38490795 Msa1325820:CDS 15.0%
!!! TATGATCCTTTAATTTTTTA+TGG - chr8_4:38490853-38490872 Msa1325820:intron 15.0%
!!! AAAATTTTATAGGAAAAACA+AGG + chr8_4:38490908-38490927 None:intergenic 15.0%
!!! AAAATTTTATAGGAAAAACA+AGG + chr8_4:38490908-38490927 None:intergenic 15.0%
!!! TTTAAGTTGCAAAATTTTAT+AGG + chr8_4:38490965-38490984 None:intergenic 15.0%
!! AATTGAATGGAAAAAACAAA+GGG + chr8_4:38490197-38490216 None:intergenic 20.0%
!! AAATTGAATGGAAAAAACAA+AGG + chr8_4:38490198-38490217 None:intergenic 20.0%
!!! TGAAATTAATTTTTTGGTCT+AGG - chr8_4:38490679-38490698 Msa1325820:CDS 20.0%
!!! GAAATTAATTTTTTGGTCTA+GGG - chr8_4:38490680-38490699 Msa1325820:CDS 20.0%
!! GAACAACCATAAAAAATTAA+AGG + chr8_4:38490862-38490881 None:intergenic 20.0%
!!! TTCAAGTTGCAAAATTTTAT+AGG + chr8_4:38490918-38490937 None:intergenic 20.0%
!! AATTGAATGGAAAAAACAAA+GGG + chr8_4:38490197-38490216 None:intergenic 20.0%
!! AAATTGAATGGAAAAAACAA+AGG + chr8_4:38490198-38490217 None:intergenic 20.0%
!!! TGAAATTAATTTTTTGGTCT+AGG - chr8_4:38490679-38490698 Msa1325820:CDS 20.0%
!!! GAAATTAATTTTTTGGTCTA+GGG - chr8_4:38490680-38490699 Msa1325820:CDS 20.0%
!! GAACAACCATAAAAAATTAA+AGG + chr8_4:38490862-38490881 None:intergenic 20.0%
!!! TTCAAGTTGCAAAATTTTAT+AGG + chr8_4:38490918-38490937 None:intergenic 20.0%
! ATTAAAGGATCATACAATAG+AGG + chr8_4:38490847-38490866 None:intergenic 25.0%
!!! TATAGTTTTCAAGTACAAGA+AGG - chr8_4:38491031-38491050 Msa1325820:intron 25.0%
!!! ATAGTTTTCAAGTACAAGAA+GGG - chr8_4:38491032-38491051 Msa1325820:intron 25.0%
! AAAAGATCAAAACTGTGAAA+AGG - chr8_4:38491187-38491206 Msa1325820:intron 25.0%
! AAAGATCAAAACTGTGAAAA+GGG - chr8_4:38491188-38491207 Msa1325820:intron 25.0%
! ATTAAAGGATCATACAATAG+AGG + chr8_4:38490847-38490866 None:intergenic 25.0%
!!! TATAGTTTTCAAGTACAAGA+AGG - chr8_4:38491031-38491050 Msa1325820:intron 25.0%
!!! ATAGTTTTCAAGTACAAGAA+GGG - chr8_4:38491032-38491051 Msa1325820:intron 25.0%
! AAAAGATCAAAACTGTGAAA+AGG - chr8_4:38491187-38491206 Msa1325820:intron 25.0%
! AAAGATCAAAACTGTGAAAA+GGG - chr8_4:38491188-38491207 Msa1325820:intron 25.0%
GTATGGTAAAGGAAATTGAA+TGG + chr8_4:38490210-38490229 None:intergenic 30.0%
GTAATATGTGATGTGTTGTA+TGG + chr8_4:38490227-38490246 None:intergenic 30.0%
GTGAAGGATATTGAATTGAA+TGG + chr8_4:38490299-38490318 None:intergenic 30.0%
ATTGAAACTGATCATAACCA+TGG + chr8_4:38490572-38490591 None:intergenic 30.0%
AGTTGAATATGTGTTTAGTG+TGG - chr8_4:38490720-38490739 Msa1325820:CDS 30.0%
!!! TTAGTGTGGATTTTGTTGTT+TGG - chr8_4:38490734-38490753 Msa1325820:CDS 30.0%
CATCTTCGAATTTCTTGATT+GGG + chr8_4:38491115-38491134 None:intergenic 30.0%
TTTCCACTGATGAAATAGAA+TGG + chr8_4:38491170-38491189 None:intergenic 30.0%
GTATGGTAAAGGAAATTGAA+TGG + chr8_4:38490210-38490229 None:intergenic 30.0%
GTAATATGTGATGTGTTGTA+TGG + chr8_4:38490227-38490246 None:intergenic 30.0%
GTGAAGGATATTGAATTGAA+TGG + chr8_4:38490299-38490318 None:intergenic 30.0%
ATTGAAACTGATCATAACCA+TGG + chr8_4:38490572-38490591 None:intergenic 30.0%
AGTTGAATATGTGTTTAGTG+TGG - chr8_4:38490720-38490739 Msa1325820:CDS 30.0%
!!! TTAGTGTGGATTTTGTTGTT+TGG - chr8_4:38490734-38490753 Msa1325820:CDS 30.0%
CATCTTCGAATTTCTTGATT+GGG + chr8_4:38491115-38491134 None:intergenic 30.0%
TTTCCACTGATGAAATAGAA+TGG + chr8_4:38491170-38491189 None:intergenic 30.0%
TGTGATGTGTTGTATGGTAA+AGG + chr8_4:38490221-38490240 None:intergenic 35.0%
! ATCCTTCACACATTTTGCTA+TGG - chr8_4:38490310-38490329 Msa1325820:CDS 35.0%
ACTGGTTGTAATTCTCAGAA+GGG + chr8_4:38490436-38490455 None:intergenic 35.0%
! TTCTGAGAATTACAACCAGT+GGG - chr8_4:38490436-38490455 Msa1325820:CDS 35.0%
TTCCAAATCAGTGACACTAT+AGG - chr8_4:38490473-38490492 Msa1325820:CDS 35.0%
! TACCTATAGTGTCACTGATT+TGG + chr8_4:38490478-38490497 None:intergenic 35.0%
CTATGTAACATACAACTCCA+TGG - chr8_4:38490552-38490571 Msa1325820:CDS 35.0%
! TTTCAAGTACAAGAAGGGTT+CGG - chr8_4:38491037-38491056 Msa1325820:intron 35.0%
CCATCTTCGAATTTCTTGAT+TGG + chr8_4:38491116-38491135 None:intergenic 35.0%
CCAATCAAGAAATTCGAAGA+TGG - chr8_4:38491113-38491132 Msa1325820:intron 35.0%
TCGTGGAGTTATAACAACAA+GGG + chr8_4:38491225-38491244 None:intergenic 35.0%
TTCGTGGAGTTATAACAACA+AGG + chr8_4:38491226-38491245 None:intergenic 35.0%
TGTGATGTGTTGTATGGTAA+AGG + chr8_4:38490221-38490240 None:intergenic 35.0%
! ATCCTTCACACATTTTGCTA+TGG - chr8_4:38490310-38490329 Msa1325820:CDS 35.0%
ACTGGTTGTAATTCTCAGAA+GGG + chr8_4:38490436-38490455 None:intergenic 35.0%
! TTCTGAGAATTACAACCAGT+GGG - chr8_4:38490436-38490455 Msa1325820:CDS 35.0%
TTCCAAATCAGTGACACTAT+AGG - chr8_4:38490473-38490492 Msa1325820:CDS 35.0%
! TACCTATAGTGTCACTGATT+TGG + chr8_4:38490478-38490497 None:intergenic 35.0%
CTATGTAACATACAACTCCA+TGG - chr8_4:38490552-38490571 Msa1325820:CDS 35.0%
! TTTCAAGTACAAGAAGGGTT+CGG - chr8_4:38491037-38491056 Msa1325820:intron 35.0%
CCATCTTCGAATTTCTTGAT+TGG + chr8_4:38491116-38491135 None:intergenic 35.0%
CCAATCAAGAAATTCGAAGA+TGG - chr8_4:38491113-38491132 Msa1325820:intron 35.0%
TCGTGGAGTTATAACAACAA+GGG + chr8_4:38491225-38491244 None:intergenic 35.0%
TTCGTGGAGTTATAACAACA+AGG + chr8_4:38491226-38491245 None:intergenic 35.0%
CTCCATAGCAAAATGTGTGA+AGG + chr8_4:38490315-38490334 None:intergenic 40.0%
GTGAAGAGAACAAAGAAGAG+AGG + chr8_4:38490347-38490366 None:intergenic 40.0%
CAAGCCTTCAAGTATTTCGT+TGG - chr8_4:38490386-38490405 Msa1325820:CDS 40.0%
TCCACCAACGAAATACTTGA+AGG + chr8_4:38490393-38490412 None:intergenic 40.0%
! CAAGTATTTCGTTGGTGGAA+AGG - chr8_4:38490394-38490413 Msa1325820:CDS 40.0%
! TATTTCGTTGGTGGAAAGGA+TGG - chr8_4:38490398-38490417 Msa1325820:CDS 40.0%
! CACTGGTTGTAATTCTCAGA+AGG + chr8_4:38490437-38490456 None:intergenic 40.0%
! CTTCTGAGAATTACAACCAG+TGG - chr8_4:38490435-38490454 Msa1325820:CDS 40.0%
TGGTGATACAGAGTTCACTT+TGG - chr8_4:38491133-38491152 Msa1325820:intron 40.0%
! CTTGGTGAAGGAGATGATTT+TGG + chr8_4:38491254-38491273 None:intergenic 40.0%
AAATCATCTCCTTCACCAAG+TGG - chr8_4:38491254-38491273 Msa1325820:intron 40.0%
ATCTAATTCAACTGCACCAC+CGG - chr8_4:38491688-38491707 Msa1325820:five_prime_UTR 40.0%
! ACTCAAAAACAGAGCACCAA+CGG + chr8_4:38491771-38491790 None:intergenic 40.0%
CTCCATAGCAAAATGTGTGA+AGG + chr8_4:38490315-38490334 None:intergenic 40.0%
GTGAAGAGAACAAAGAAGAG+AGG + chr8_4:38490347-38490366 None:intergenic 40.0%
CAAGCCTTCAAGTATTTCGT+TGG - chr8_4:38490386-38490405 Msa1325820:CDS 40.0%
TCCACCAACGAAATACTTGA+AGG + chr8_4:38490393-38490412 None:intergenic 40.0%
! CAAGTATTTCGTTGGTGGAA+AGG - chr8_4:38490394-38490413 Msa1325820:CDS 40.0%
! TATTTCGTTGGTGGAAAGGA+TGG - chr8_4:38490398-38490417 Msa1325820:CDS 40.0%
! CACTGGTTGTAATTCTCAGA+AGG + chr8_4:38490437-38490456 None:intergenic 40.0%
! CTTCTGAGAATTACAACCAG+TGG - chr8_4:38490435-38490454 Msa1325820:CDS 40.0%
TGGTGATACAGAGTTCACTT+TGG - chr8_4:38491133-38491152 Msa1325820:intron 40.0%
! CTTGGTGAAGGAGATGATTT+TGG + chr8_4:38491254-38491273 None:intergenic 40.0%
AAATCATCTCCTTCACCAAG+TGG - chr8_4:38491254-38491273 Msa1325820:intron 40.0%
ATCTAATTCAACTGCACCAC+CGG - chr8_4:38491688-38491707 Msa1325820:five_prime_UTR 40.0%
! ACTCAAAAACAGAGCACCAA+CGG + chr8_4:38491771-38491790 None:intergenic 40.0%
GCCTTCAAGTATTTCGTTGG+TGG - chr8_4:38490389-38490408 Msa1325820:CDS 45.0%
AGTTCACTTTGGACAGAGCA+GGG - chr8_4:38491144-38491163 Msa1325820:intron 45.0%
GGGCCATTCTATTTCATCAG+TGG - chr8_4:38491164-38491183 Msa1325820:intron 45.0%
!!! GATGATTTTGGTGGTGTTCG+TGG + chr8_4:38491242-38491261 None:intergenic 45.0%
! GGTGAAGGAGATGATTTTGG+TGG + chr8_4:38491251-38491270 None:intergenic 45.0%
GGTGCAGTTGAATTAGATCC+TGG + chr8_4:38491686-38491705 None:intergenic 45.0%
TCTAATTCAACTGCACCACC+GGG - chr8_4:38491689-38491708 Msa1325820:five_prime_UTR 45.0%
ACAAATGAACCACTGCTACC+CGG + chr8_4:38491710-38491729 None:intergenic 45.0%
GAATACACCAGAAAGCTCGA+AGG + chr8_4:38491737-38491756 None:intergenic 45.0%
GCCTTCAAGTATTTCGTTGG+TGG - chr8_4:38490389-38490408 Msa1325820:CDS 45.0%
AGTTCACTTTGGACAGAGCA+GGG - chr8_4:38491144-38491163 Msa1325820:intron 45.0%
GGGCCATTCTATTTCATCAG+TGG - chr8_4:38491164-38491183 Msa1325820:intron 45.0%
!!! GATGATTTTGGTGGTGTTCG+TGG + chr8_4:38491242-38491261 None:intergenic 45.0%
! GGTGAAGGAGATGATTTTGG+TGG + chr8_4:38491251-38491270 None:intergenic 45.0%
GGTGCAGTTGAATTAGATCC+TGG + chr8_4:38491686-38491705 None:intergenic 45.0%
TCTAATTCAACTGCACCACC+GGG - chr8_4:38491689-38491708 Msa1325820:five_prime_UTR 45.0%
ACAAATGAACCACTGCTACC+CGG + chr8_4:38491710-38491729 None:intergenic 45.0%
GAATACACCAGAAAGCTCGA+AGG + chr8_4:38491737-38491756 None:intergenic 45.0%
!! AATTAAATTAATATTAACAT+GGG + chr8_4:38490507-38490526 None:intergenic 5.0%
!! AAATTAAATTAATATTAACA+TGG + chr8_4:38490508-38490527 None:intergenic 5.0%
!!! TATATATGAAATTAATTTTT+TGG - chr8_4:38490673-38490692 Msa1325820:CDS 5.0%
!! AATTAAATTAATATTAACAT+GGG + chr8_4:38490507-38490526 None:intergenic 5.0%
!! AAATTAAATTAATATTAACA+TGG + chr8_4:38490508-38490527 None:intergenic 5.0%
!!! TATATATGAAATTAATTTTT+TGG - chr8_4:38490673-38490692 Msa1325820:CDS 5.0%
! TCGTTGGTGGAAAGGATGGA+TGG - chr8_4:38490402-38490421 Msa1325820:CDS 50.0%
GAGAATTACAACCAGTGGGC+TGG - chr8_4:38490440-38490459 Msa1325820:CDS 50.0%
!! ATTACAACCAGTGGGCTGGA+AGG - chr8_4:38490444-38490463 Msa1325820:CDS 50.0%
GTACAAGAAGGGTTCGGACT+CGG - chr8_4:38491043-38491062 Msa1325820:intron 50.0%
GAGTTCACTTTGGACAGAGC+AGG - chr8_4:38491143-38491162 Msa1325820:intron 50.0%
TCATCTCCTTCACCAAGTGG+TGG - chr8_4:38491257-38491276 Msa1325820:intron 50.0%
!! TGAAACAGAGGGTGATGGAG+TGG + chr8_4:38491318-38491337 None:intergenic 50.0%
! GGAGATGGTGGTGAAACAGA+GGG + chr8_4:38491329-38491348 None:intergenic 50.0%
! TGGAGATGGTGGTGAAACAG+AGG + chr8_4:38491330-38491349 None:intergenic 50.0%
TGTTTCACCACCATCTCCAG+CGG - chr8_4:38491331-38491350 Msa1325820:intron 50.0%
GGAGATGGAGATGATGCCAT+AGG + chr8_4:38491446-38491465 None:intergenic 50.0%
CTCACTACTGGAGATGGAGA+TGG + chr8_4:38491404-38491423 None:intergenic 50.0%
TACGGCTTCATCTCCTTCTC+CGG - chr8_4:38491502-38491521 Msa1325820:CDS 50.0%
TGTAGCTCCTTCGAGCTTTC+TGG - chr8_4:38491727-38491746 Msa1325820:five_prime_UTR 50.0%
! AAACAGAGCACCAACGGCAA+GGG + chr8_4:38491765-38491784 None:intergenic 50.0%
! AAAACAGAGCACCAACGGCA+AGG + chr8_4:38491766-38491785 None:intergenic 50.0%
! TCGTTGGTGGAAAGGATGGA+TGG - chr8_4:38490402-38490421 Msa1325820:CDS 50.0%
GAGAATTACAACCAGTGGGC+TGG - chr8_4:38490440-38490459 Msa1325820:CDS 50.0%
!! ATTACAACCAGTGGGCTGGA+AGG - chr8_4:38490444-38490463 Msa1325820:CDS 50.0%
GTACAAGAAGGGTTCGGACT+CGG - chr8_4:38491043-38491062 Msa1325820:intron 50.0%
GAGTTCACTTTGGACAGAGC+AGG - chr8_4:38491143-38491162 Msa1325820:intron 50.0%
TCATCTCCTTCACCAAGTGG+TGG - chr8_4:38491257-38491276 Msa1325820:intron 50.0%
!! TGAAACAGAGGGTGATGGAG+TGG + chr8_4:38491318-38491337 None:intergenic 50.0%
! GGAGATGGTGGTGAAACAGA+GGG + chr8_4:38491329-38491348 None:intergenic 50.0%
! TGGAGATGGTGGTGAAACAG+AGG + chr8_4:38491330-38491349 None:intergenic 50.0%
TGTTTCACCACCATCTCCAG+CGG - chr8_4:38491331-38491350 Msa1325820:intron 50.0%
GGAGATGGAGATGATGCCAT+AGG + chr8_4:38491446-38491465 None:intergenic 50.0%
CTCACTACTGGAGATGGAGA+TGG + chr8_4:38491404-38491423 None:intergenic 50.0%
TACGGCTTCATCTCCTTCTC+CGG - chr8_4:38491502-38491521 Msa1325820:CDS 50.0%
TGTAGCTCCTTCGAGCTTTC+TGG - chr8_4:38491727-38491746 Msa1325820:five_prime_UTR 50.0%
! AAACAGAGCACCAACGGCAA+GGG + chr8_4:38491765-38491784 None:intergenic 50.0%
! AAAACAGAGCACCAACGGCA+AGG + chr8_4:38491766-38491785 None:intergenic 50.0%
TCTCCTTCACCAAGTGGTGG+TGG - chr8_4:38491260-38491279 Msa1325820:intron 55.0%
! AAGCCACCACCACTTGGTGA+AGG + chr8_4:38491266-38491285 None:intergenic 55.0%
GGTGATAAGCCACCACCACT+TGG + chr8_4:38491272-38491291 None:intergenic 55.0%
!! GTTGTCGTTGGTGACGGTGA+AGG + chr8_4:38491296-38491315 None:intergenic 55.0%
! GGAGTGGTTGTCGTTGGTGA+CGG + chr8_4:38491302-38491321 None:intergenic 55.0%
!! GGTGATGGAGTGGTTGTCGT+TGG + chr8_4:38491308-38491327 None:intergenic 55.0%
GGTGGTGAAACAGAGGGTGA+TGG + chr8_4:38491323-38491342 None:intergenic 55.0%
AGAAGATGCCGTAGGTCCAC+CGG + chr8_4:38491387-38491406 None:intergenic 55.0%
GGAGATGGAGAAGATGCCGT+AGG + chr8_4:38491395-38491414 None:intergenic 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_4:38491410-38491429 None:intergenic 55.0%
TCCAGTAGTGAGCACACCTC+CGG - chr8_4:38491412-38491431 Msa1325820:CDS 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_4:38491410-38491429 None:intergenic 55.0%
AGATGAAGCCGTAGGTCCAC+CGG + chr8_4:38491495-38491514 None:intergenic 55.0%
GGAGAAGGAGATGAAGCCGT+AGG + chr8_4:38491503-38491522 None:intergenic 55.0%
ATCTACACCAGGTGGTCCTC+CGG - chr8_4:38491562-38491581 Msa1325820:five_prime_UTR 55.0%
AATGAACCACTGCTACCCGG+TGG + chr8_4:38491707-38491726 None:intergenic 55.0%
TATTCCGCAACCCTTGCCGT+TGG - chr8_4:38491752-38491771 Msa1325820:five_prime_UTR 55.0%
TCTCCTTCACCAAGTGGTGG+TGG - chr8_4:38491260-38491279 Msa1325820:intron 55.0%
! AAGCCACCACCACTTGGTGA+AGG + chr8_4:38491266-38491285 None:intergenic 55.0%
GGTGATAAGCCACCACCACT+TGG + chr8_4:38491272-38491291 None:intergenic 55.0%
!! GTTGTCGTTGGTGACGGTGA+AGG + chr8_4:38491296-38491315 None:intergenic 55.0%
! GGAGTGGTTGTCGTTGGTGA+CGG + chr8_4:38491302-38491321 None:intergenic 55.0%
!! GGTGATGGAGTGGTTGTCGT+TGG + chr8_4:38491308-38491327 None:intergenic 55.0%
GGTGGTGAAACAGAGGGTGA+TGG + chr8_4:38491323-38491342 None:intergenic 55.0%
AGAAGATGCCGTAGGTCCAC+CGG + chr8_4:38491387-38491406 None:intergenic 55.0%
GGAGATGGAGAAGATGCCGT+AGG + chr8_4:38491395-38491414 None:intergenic 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_4:38491410-38491429 None:intergenic 55.0%
TCCAGTAGTGAGCACACCTC+CGG - chr8_4:38491412-38491431 Msa1325820:CDS 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_4:38491410-38491429 None:intergenic 55.0%
AGATGAAGCCGTAGGTCCAC+CGG + chr8_4:38491495-38491514 None:intergenic 55.0%
GGAGAAGGAGATGAAGCCGT+AGG + chr8_4:38491503-38491522 None:intergenic 55.0%
ATCTACACCAGGTGGTCCTC+CGG - chr8_4:38491562-38491581 Msa1325820:five_prime_UTR 55.0%
AATGAACCACTGCTACCCGG+TGG + chr8_4:38491707-38491726 None:intergenic 55.0%
TATTCCGCAACCCTTGCCGT+TGG - chr8_4:38491752-38491771 Msa1325820:five_prime_UTR 55.0%
!! ACCAGTGGGCTGGAAGGAAC+AGG - chr8_4:38490450-38490469 Msa1325820:CDS 60.0%
ACCTGTTCCTTCCAGCCCAC+TGG + chr8_4:38490454-38490473 None:intergenic 60.0%
!! GAAGGGTTCGGACTCGGTGT+TGG - chr8_4:38491049-38491068 Msa1325820:intron 60.0%
!! GTCGTTGGTGACGGTGAAGG+TGG + chr8_4:38491293-38491312 None:intergenic 60.0%
GGAATAGCCGCTGGAGATGG+TGG + chr8_4:38491341-38491360 None:intergenic 60.0%
GGAGGAATAGCCGCTGGAGA+TGG + chr8_4:38491344-38491363 None:intergenic 60.0%
CTTGCTGGAGGAATAGCCGC+TGG + chr8_4:38491350-38491369 None:intergenic 60.0%
! TAGGTCCACCGGCTGGAACA+GGG + chr8_4:38491376-38491395 None:intergenic 60.0%
TGTTCCAGCCGGTGGACCTA+CGG - chr8_4:38491376-38491395 Msa1325820:intron 60.0%
GATGCCATAGGTCCACCAGC+CGG + chr8_4:38491434-38491453 None:intergenic 60.0%
! GCTGGAGGTGTGCTCACTAC+TGG + chr8_4:38491473-38491492 None:intergenic 60.0%
GTAGTGAGCACACCTCCAGC+CGG - chr8_4:38491473-38491492 Msa1325820:CDS 60.0%
GCTTCATCTCCTTCTCCGGC+AGG - chr8_4:38491506-38491525 Msa1325820:CDS 60.0%
TCATCTCCTTCTCCGGCAGG+TGG - chr8_4:38491509-38491528 Msa1325820:CDS 60.0%
GATGGACCACCTGCTGGTGA+AGG + chr8_4:38491545-38491564 None:intergenic 60.0%
GGTGTAGATGGACCACCTGC+TGG + chr8_4:38491551-38491570 None:intergenic 60.0%
GCAGGTGGTCCATCTACACC+AGG - chr8_4:38491551-38491570 Msa1325820:exon 60.0%
GGTGGTCCATCTACACCAGG+TGG - chr8_4:38491554-38491573 Msa1325820:exon 60.0%
GGAGGACCACCTGGTGTAGA+TGG + chr8_4:38491563-38491582 None:intergenic 60.0%
TTAGATCCTGGTGACCCCGC+TGG + chr8_4:38491674-38491693 None:intergenic 60.0%
AGCACCAACGGCAAGGGTTG+CGG + chr8_4:38491759-38491778 None:intergenic 60.0%
!! ACCAGTGGGCTGGAAGGAAC+AGG - chr8_4:38490450-38490469 Msa1325820:CDS 60.0%
ACCTGTTCCTTCCAGCCCAC+TGG + chr8_4:38490454-38490473 None:intergenic 60.0%
!! GAAGGGTTCGGACTCGGTGT+TGG - chr8_4:38491049-38491068 Msa1325820:intron 60.0%
!! GTCGTTGGTGACGGTGAAGG+TGG + chr8_4:38491293-38491312 None:intergenic 60.0%
GGAATAGCCGCTGGAGATGG+TGG + chr8_4:38491341-38491360 None:intergenic 60.0%
GGAGGAATAGCCGCTGGAGA+TGG + chr8_4:38491344-38491363 None:intergenic 60.0%
CTTGCTGGAGGAATAGCCGC+TGG + chr8_4:38491350-38491369 None:intergenic 60.0%
! TAGGTCCACCGGCTGGAACA+GGG + chr8_4:38491376-38491395 None:intergenic 60.0%
TGTTCCAGCCGGTGGACCTA+CGG - chr8_4:38491376-38491395 Msa1325820:intron 60.0%
GATGCCATAGGTCCACCAGC+CGG + chr8_4:38491434-38491453 None:intergenic 60.0%
! GCTGGAGGTGTGCTCACTAC+TGG + chr8_4:38491473-38491492 None:intergenic 60.0%
GTAGTGAGCACACCTCCAGC+CGG - chr8_4:38491473-38491492 Msa1325820:CDS 60.0%
GCTTCATCTCCTTCTCCGGC+AGG - chr8_4:38491506-38491525 Msa1325820:CDS 60.0%
TCATCTCCTTCTCCGGCAGG+TGG - chr8_4:38491509-38491528 Msa1325820:CDS 60.0%
GATGGACCACCTGCTGGTGA+AGG + chr8_4:38491545-38491564 None:intergenic 60.0%
GGTGTAGATGGACCACCTGC+TGG + chr8_4:38491551-38491570 None:intergenic 60.0%
GCAGGTGGTCCATCTACACC+AGG - chr8_4:38491551-38491570 Msa1325820:exon 60.0%
GGTGGTCCATCTACACCAGG+TGG - chr8_4:38491554-38491573 Msa1325820:exon 60.0%
GGAGGACCACCTGGTGTAGA+TGG + chr8_4:38491563-38491582 None:intergenic 60.0%
TTAGATCCTGGTGACCCCGC+TGG + chr8_4:38491674-38491693 None:intergenic 60.0%
AGCACCAACGGCAAGGGTTG+CGG + chr8_4:38491759-38491778 None:intergenic 60.0%
!! GGAACAGGGGAGCTTGCTGG+AGG + chr8_4:38491362-38491381 None:intergenic 65.0%
GCTGGAACAGGGGAGCTTGC+TGG + chr8_4:38491365-38491384 None:intergenic 65.0%
GCAAGCTCCCCTGTTCCAGC+CGG - chr8_4:38491365-38491384 Msa1325820:intron 65.0%
! AGGTCCACCGGCTGGAACAG+GGG + chr8_4:38491375-38491394 None:intergenic 65.0%
! GTAGGTCCACCGGCTGGAAC+AGG + chr8_4:38491377-38491396 None:intergenic 65.0%
! GCCGGAGGTGTGCTCACTAC+TGG + chr8_4:38491416-38491435 None:intergenic 65.0%
GTAGTGAGCACACCTCCGGC+TGG - chr8_4:38491416-38491435 Msa1325820:CDS 65.0%
! ACCTCCGGCTGGTGGACCTA+TGG - chr8_4:38491427-38491446 Msa1325820:CDS 65.0%
ACCTCCAGCCGGTGGACCTA+CGG - chr8_4:38491484-38491503 Msa1325820:CDS 65.0%
CCTGCTGGTGAAGGAGCTGG+TGG + chr8_4:38491536-38491555 None:intergenic 65.0%
CCACCAGCTCCTTCACCAGC+AGG - chr8_4:38491533-38491552 Msa1325820:CDS 65.0%
CCACCTGCTGGTGAAGGAGC+TGG + chr8_4:38491539-38491558 None:intergenic 65.0%
CCAGCTCCTTCACCAGCAGG+TGG - chr8_4:38491536-38491555 Msa1325820:exon 65.0%
TGATGGAGTCGCTGCTCCCC+CGG + chr8_4:38491597-38491616 None:intergenic 65.0%
GGGGAGCAGCGACTCCATCA+GGG - chr8_4:38491597-38491616 Msa1325820:five_prime_UTR 65.0%
GGGAGCAGCGACTCCATCAG+GGG - chr8_4:38491598-38491617 Msa1325820:five_prime_UTR 65.0%
TCCTGGTGCAGCTGATCCCC+CGG + chr8_4:38491633-38491652 None:intergenic 65.0%
GGATCAGCTGCACCAGGACC+CGG - chr8_4:38491635-38491654 Msa1325820:five_prime_UTR 65.0%
GATCAGCTGCACCAGGACCC+GGG - chr8_4:38491636-38491655 Msa1325820:five_prime_UTR 65.0%
ATCAGCTGCACCAGGACCCG+GGG - chr8_4:38491637-38491656 Msa1325820:five_prime_UTR 65.0%
ACTGCACCACCGGGTAGCAG+TGG - chr8_4:38491698-38491717 Msa1325820:five_prime_UTR 65.0%
!! GGAACAGGGGAGCTTGCTGG+AGG + chr8_4:38491362-38491381 None:intergenic 65.0%
GCTGGAACAGGGGAGCTTGC+TGG + chr8_4:38491365-38491384 None:intergenic 65.0%
GCAAGCTCCCCTGTTCCAGC+CGG - chr8_4:38491365-38491384 Msa1325820:intron 65.0%
! AGGTCCACCGGCTGGAACAG+GGG + chr8_4:38491375-38491394 None:intergenic 65.0%
! GTAGGTCCACCGGCTGGAAC+AGG + chr8_4:38491377-38491396 None:intergenic 65.0%
! GCCGGAGGTGTGCTCACTAC+TGG + chr8_4:38491416-38491435 None:intergenic 65.0%
GTAGTGAGCACACCTCCGGC+TGG - chr8_4:38491416-38491435 Msa1325820:CDS 65.0%
! ACCTCCGGCTGGTGGACCTA+TGG - chr8_4:38491427-38491446 Msa1325820:CDS 65.0%
ACCTCCAGCCGGTGGACCTA+CGG - chr8_4:38491484-38491503 Msa1325820:CDS 65.0%
CCTGCTGGTGAAGGAGCTGG+TGG + chr8_4:38491536-38491555 None:intergenic 65.0%
CCACCAGCTCCTTCACCAGC+AGG - chr8_4:38491533-38491552 Msa1325820:CDS 65.0%
CCACCTGCTGGTGAAGGAGC+TGG + chr8_4:38491539-38491558 None:intergenic 65.0%
CCAGCTCCTTCACCAGCAGG+TGG - chr8_4:38491536-38491555 Msa1325820:exon 65.0%
TGATGGAGTCGCTGCTCCCC+CGG + chr8_4:38491597-38491616 None:intergenic 65.0%
GGGGAGCAGCGACTCCATCA+GGG - chr8_4:38491597-38491616 Msa1325820:five_prime_UTR 65.0%
GGGAGCAGCGACTCCATCAG+GGG - chr8_4:38491598-38491617 Msa1325820:five_prime_UTR 65.0%
TCCTGGTGCAGCTGATCCCC+CGG + chr8_4:38491633-38491652 None:intergenic 65.0%
GGATCAGCTGCACCAGGACC+CGG - chr8_4:38491635-38491654 Msa1325820:five_prime_UTR 65.0%
GATCAGCTGCACCAGGACCC+GGG - chr8_4:38491636-38491655 Msa1325820:five_prime_UTR 65.0%
ATCAGCTGCACCAGGACCCG+GGG - chr8_4:38491637-38491656 Msa1325820:five_prime_UTR 65.0%
ACTGCACCACCGGGTAGCAG+TGG - chr8_4:38491698-38491717 Msa1325820:five_prime_UTR 65.0%
AGCTCCCCTGTTCCAGCCGG+TGG - chr8_4:38491368-38491387 Msa1325820:intron 70.0%
GATGCCGTAGGTCCACCGGC+TGG + chr8_4:38491383-38491402 None:intergenic 70.0%
GTGAGCACACCTCCGGCTGG+TGG - chr8_4:38491419-38491438 Msa1325820:CDS 70.0%
GCCATAGGTCCACCAGCCGG+AGG + chr8_4:38491431-38491450 None:intergenic 70.0%
GTGAGCACACCTCCAGCCGG+TGG - chr8_4:38491476-38491495 Msa1325820:CDS 70.0%
GAAGCCGTAGGTCCACCGGC+TGG + chr8_4:38491491-38491510 None:intergenic 70.0%
GGTGGTCCACCTGCCGGAGA+AGG + chr8_4:38491518-38491537 None:intergenic 70.0%
GGAGCTGGTGGTCCACCTGC+CGG + chr8_4:38491524-38491543 None:intergenic 70.0%
GATGGAGTCGCTGCTCCCCC+GGG + chr8_4:38491596-38491615 None:intergenic 70.0%
GGGGGAGCAGCGACTCCATC+AGG - chr8_4:38491596-38491615 Msa1325820:five_prime_UTR 70.0%
!! GCGACTCCATCAGGGGCACC+AGG - chr8_4:38491605-38491624 Msa1325820:five_prime_UTR 70.0%
!! CCATCAGGGGCACCAGGACC+CGG - chr8_4:38491611-38491630 Msa1325820:five_prime_UTR 70.0%
!! CATCAGGGGCACCAGGACCC+GGG - chr8_4:38491612-38491631 Msa1325820:five_prime_UTR 70.0%
!! ATCAGGGGCACCAGGACCCG+GGG - chr8_4:38491613-38491632 Msa1325820:five_prime_UTR 70.0%
CCTGGTGCAGCTGATCCCCC+GGG + chr8_4:38491632-38491651 None:intergenic 70.0%
TCAGCTGCACCAGGACCCGG+GGG - chr8_4:38491638-38491657 Msa1325820:five_prime_UTR 70.0%
AGCTCCCCTGTTCCAGCCGG+TGG - chr8_4:38491368-38491387 Msa1325820:intron 70.0%
GATGCCGTAGGTCCACCGGC+TGG + chr8_4:38491383-38491402 None:intergenic 70.0%
GTGAGCACACCTCCGGCTGG+TGG - chr8_4:38491419-38491438 Msa1325820:CDS 70.0%
GCCATAGGTCCACCAGCCGG+AGG + chr8_4:38491431-38491450 None:intergenic 70.0%
GTGAGCACACCTCCAGCCGG+TGG - chr8_4:38491476-38491495 Msa1325820:CDS 70.0%
GAAGCCGTAGGTCCACCGGC+TGG + chr8_4:38491491-38491510 None:intergenic 70.0%
GGTGGTCCACCTGCCGGAGA+AGG + chr8_4:38491518-38491537 None:intergenic 70.0%
GGAGCTGGTGGTCCACCTGC+CGG + chr8_4:38491524-38491543 None:intergenic 70.0%
GATGGAGTCGCTGCTCCCCC+GGG + chr8_4:38491596-38491615 None:intergenic 70.0%
GGGGGAGCAGCGACTCCATC+AGG - chr8_4:38491596-38491615 Msa1325820:five_prime_UTR 70.0%
!! GCGACTCCATCAGGGGCACC+AGG - chr8_4:38491605-38491624 Msa1325820:five_prime_UTR 70.0%
!! CCATCAGGGGCACCAGGACC+CGG - chr8_4:38491611-38491630 Msa1325820:five_prime_UTR 70.0%
!! CATCAGGGGCACCAGGACCC+GGG - chr8_4:38491612-38491631 Msa1325820:five_prime_UTR 70.0%
!! ATCAGGGGCACCAGGACCCG+GGG - chr8_4:38491613-38491632 Msa1325820:five_prime_UTR 70.0%
CCTGGTGCAGCTGATCCCCC+GGG + chr8_4:38491632-38491651 None:intergenic 70.0%
TCAGCTGCACCAGGACCCGG+GGG - chr8_4:38491638-38491657 Msa1325820:five_prime_UTR 70.0%
! GCCGTAGGTCCACCGGCTGG+AGG + chr8_4:38491380-38491399 None:intergenic 75.0%
! CCTGGTGCCGGAGGACCACC+TGG + chr8_4:38491572-38491591 None:intergenic 75.0%
CCAGGTGGTCCTCCGGCACC+AGG - chr8_4:38491569-38491588 Msa1325820:five_prime_UTR 75.0%
GGTCCTCCGGCACCAGGACC+CGG - chr8_4:38491575-38491594 Msa1325820:five_prime_UTR 75.0%
GTCCTCCGGCACCAGGACCC+GGG - chr8_4:38491576-38491595 Msa1325820:five_prime_UTR 75.0%
TCCTCCGGCACCAGGACCCG+GGG - chr8_4:38491577-38491596 Msa1325820:five_prime_UTR 75.0%
CCGGGTCCTGGTGCCCCTGA+TGG + chr8_4:38491614-38491633 None:intergenic 75.0%
!! TCAGGGGCACCAGGACCCGG+GGG - chr8_4:38491614-38491633 Msa1325820:five_prime_UTR 75.0%
GCAGCTGATCCCCCGGGTCC+TGG + chr8_4:38491626-38491645 None:intergenic 75.0%
CCCGGGGGATCAGCTGCACC+AGG - chr8_4:38491629-38491648 Msa1325820:five_prime_UTR 75.0%
GCTGGAGCTGCTGCTCCCCC+GGG + chr8_4:38491656-38491675 None:intergenic 75.0%
CGCTGGAGCTGCTGCTCCCC+CGG + chr8_4:38491657-38491676 None:intergenic 75.0%
CGGGGGAGCAGCAGCTCCAG+CGG - chr8_4:38491655-38491674 Msa1325820:five_prime_UTR 75.0%
GGGGGAGCAGCAGCTCCAGC+GGG - chr8_4:38491656-38491675 Msa1325820:five_prime_UTR 75.0%
GGGGAGCAGCAGCTCCAGCG+GGG - chr8_4:38491657-38491676 Msa1325820:five_prime_UTR 75.0%
GCAGCTCCAGCGGGGTCACC+AGG - chr8_4:38491665-38491684 Msa1325820:five_prime_UTR 75.0%
! GCCGTAGGTCCACCGGCTGG+AGG + chr8_4:38491380-38491399 None:intergenic 75.0%
! CCTGGTGCCGGAGGACCACC+TGG + chr8_4:38491572-38491591 None:intergenic 75.0%
CCAGGTGGTCCTCCGGCACC+AGG - chr8_4:38491569-38491588 Msa1325820:five_prime_UTR 75.0%
GGTCCTCCGGCACCAGGACC+CGG - chr8_4:38491575-38491594 Msa1325820:five_prime_UTR 75.0%
GTCCTCCGGCACCAGGACCC+GGG - chr8_4:38491576-38491595 Msa1325820:five_prime_UTR 75.0%
TCCTCCGGCACCAGGACCCG+GGG - chr8_4:38491577-38491596 Msa1325820:five_prime_UTR 75.0%
CCGGGTCCTGGTGCCCCTGA+TGG + chr8_4:38491614-38491633 None:intergenic 75.0%
!! TCAGGGGCACCAGGACCCGG+GGG - chr8_4:38491614-38491633 Msa1325820:five_prime_UTR 75.0%
GCAGCTGATCCCCCGGGTCC+TGG + chr8_4:38491626-38491645 None:intergenic 75.0%
CCCGGGGGATCAGCTGCACC+AGG - chr8_4:38491629-38491648 Msa1325820:five_prime_UTR 75.0%
GCTGGAGCTGCTGCTCCCCC+GGG + chr8_4:38491656-38491675 None:intergenic 75.0%
CGCTGGAGCTGCTGCTCCCC+CGG + chr8_4:38491657-38491676 None:intergenic 75.0%
CGGGGGAGCAGCAGCTCCAG+CGG - chr8_4:38491655-38491674 Msa1325820:five_prime_UTR 75.0%
GGGGGAGCAGCAGCTCCAGC+GGG - chr8_4:38491656-38491675 Msa1325820:five_prime_UTR 75.0%
GGGGAGCAGCAGCTCCAGCG+GGG - chr8_4:38491657-38491676 Msa1325820:five_prime_UTR 75.0%
GCAGCTCCAGCGGGGTCACC+AGG - chr8_4:38491665-38491684 Msa1325820:five_prime_UTR 75.0%
! CCTCCGGCACCAGGACCCGG+GGG - chr8_4:38491578-38491597 Msa1325820:five_prime_UTR 80.0%
! GCTCCCCCGGGTCCTGGTGC+CGG + chr8_4:38491584-38491603 None:intergenic 80.0%
! GTCGCTGCTCCCCCGGGTCC+TGG + chr8_4:38491590-38491609 None:intergenic 80.0%
! GCTGCTGCTCCCCCGGGTCC+TGG + chr8_4:38491650-38491669 None:intergenic 80.0%
! CCTCCGGCACCAGGACCCGG+GGG - chr8_4:38491578-38491597 Msa1325820:five_prime_UTR 80.0%
! GCTCCCCCGGGTCCTGGTGC+CGG + chr8_4:38491584-38491603 None:intergenic 80.0%
! GTCGCTGCTCCCCCGGGTCC+TGG + chr8_4:38491590-38491609 None:intergenic 80.0%
! GCTGCTGCTCCCCCGGGTCC+TGG + chr8_4:38491650-38491669 None:intergenic 80.0%
!! CCCCCGGGTCCTGGTGCCGG+AGG + chr8_4:38491581-38491600 None:intergenic 85.0%
!! CCCCCGGGTCCTGGTGCCGG+AGG + chr8_4:38491581-38491600 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 38490087 38491796 38490087 ID=Msa1325820;Name=Msa1325820
chr8_4 mRNA 38490087 38491796 38490087 ID=Msa1325820-mRNA-1;Parent=Msa1325820;Name=Msa1325820-mRNA-1;_AED=0.01;_eAED=0.01;_QI=242|1|0.5|1|1|1|2|0|308
chr8_4 exon 38490087 38490847 38490087 ID=Msa1325820-mRNA-1:exon:11712;Parent=Msa1325820-mRNA-1
chr8_4 exon 38491389 38491796 38491389 ID=Msa1325820-mRNA-1:exon:11711;Parent=Msa1325820-mRNA-1
chr8_4 five_prime_UTR 38491555 38491796 38491555 ID=Msa1325820-mRNA-1:five_prime_utr;Parent=Msa1325820-mRNA-1
chr8_4 CDS 38491389 38491554 38491389 ID=Msa1325820-mRNA-1:cds;Parent=Msa1325820-mRNA-1
chr8_4 CDS 38490087 38490847 38490087 ID=Msa1325820-mRNA-1:cds;Parent=Msa1325820-mRNA-1
Gene Sequence

>Msa1325820

ATGGAGTTTAAGATACCTCTCTTCTTTGTTCTCTTCACTCTTGTTGTCTCAACTTCTCAAGCCTTCAAGTATTTCGTTGGTGGAAAGGATGGATGGACTCTAAACCCTTCTGAGAATTACAACCAGTGGGCTGGAAGGAACAGGTTCCAAATCAGTGACACTATAGTTTTCAAGTACAAGAAGGGTTCGGACTCGGTGTTGGAAGTGAAGAAAGAAGATTATGAAAAGTGCAACAAAACAAACCCAATCAAGAAATTCGAAGATGGTGATACAGAGTTCACTTTGGACAGAGCAGGGCCATTCTATTTCATCAGTGGAAAAGATCAAAACTGTGAAAAGGGTCAGAAATTAACCCTTGTTGTTATAACTCCACGAACACCACCAAAATCATCTCCTTCACCAAGTGGTGGTGGCTTATCACCACCTTCACCGTCACCAACGACAACCACTCCATCACCCTCTGTTTCACCACCATCTCCAGCGGCTATTCCTCCAGCAAGCTCCCCTGTTCCAGCCGGTGGACCTACGGCATCTTCTCCATCTCCAGTAGTGAGCACACCTCCGGCTGGTGGACCTATGGCATCATCTCCATCTCCATCTCCAGTAGTGAGCACACCTCCAGCCGGTGGACCTACGGCTTCATCTCCTTCTCCGGCAGGTGGACCACCAGCTCCTTCACCAGCAGGTGGTCCATCTACACCAGGTGGTCCTCCGGCACCAGGACCCGGGGGAGCAGCGACTCCATCAGGGGCACCAGGACCCGGGGGATCAGCTGCACCAGGACCCGGGGGAGCAGCAGCTCCAGCGGGGTCACCAGGATCTAATTCAACTGCACCACCGGGTAGCAGTGGTTCATTTGTAGCTCCTTCGAGCTTTCTGGTGTATTCCGCAACCCTTGCCGTTGGTGCTCTGTTTTTGAGTTACTGA
Protein sequence

>Msa1325820

MEFKIPLFFVLFTLVVSTSQAFKYFVGGKDGWTLNPSENYNQWAGRNRFQISDTIVFKYKKGSDSVLEVKKEDYEKCNKTNPIKKFEDGDTEFTLDRAGPFYFISGKDQNCEKGQKLTLVVITPRTPPKSSPSPSGGGLSPPSPSPTTTTPSPSVSPPSPAAIPPASSPVPAGGPTASSPSPVVSTPPAGGPMASSPSPSPVVSTPPAGGPTASSPSPAGGPPAPSPAGGPSTPGGPPAPGPGGAATPSGAPGPGGSAAPGPGGAAAPAGSPGSNSTAPPGSSGSFVAPSSFLVYSATLAVGALFLSY*