AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1330050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330050 MtrunA17_Chr4g0060981 95.890 219 9 0 1 219 1 219 9.62e-149 412
Msa1330050 MtrunA17_Chr5g0406031 40.404 198 111 2 25 219 41 234 9.26e-52 167
Msa1330050 MtrunA17_Chr8g0371291 38.095 210 123 3 8 217 21 223 2.03e-46 153
Msa1330050 MtrunA17_Chr5g0444161 35.437 206 114 6 17 219 41 230 5.37e-27 103
Msa1330050 MtrunA17_Chr5g0444141 35.533 197 112 6 27 219 80 265 8.38e-26 101
Msa1330050 MtrunA17_Chr6g0451051 46.575 73 38 1 148 219 39 111 5.26e-16 72.4
Msa1330050 MtrunA17_Chr3g0128771 33.333 93 59 1 130 219 72 164 2.64e-15 71.2
Msa1330050 MtrunA17_Chr5g0437361 45.833 72 36 1 148 216 74 145 4.63e-15 70.1
Msa1330050 MtrunA17_Chr8g0383941 36.735 98 52 2 122 217 88 177 1.14e-14 70.1
Msa1330050 MtrunA17_Chr8g0383881 43.056 72 39 1 148 217 102 173 3.44e-14 68.9
Msa1330050 MtrunA17_Chr8g0383861 43.056 72 39 1 148 217 112 183 3.83e-14 68.9
Msa1330050 MtrunA17_Chr8g0388231 39.726 73 41 1 149 218 309 381 5.63e-14 70.1
Msa1330050 MtrunA17_Chr7g0246761 55.319 47 21 0 170 216 86 132 5.69e-14 67.8
Msa1330050 MtrunA17_Chr6g0451031 41.667 72 42 0 148 219 43 114 8.33e-14 65.9
Msa1330050 MtrunA17_Chr3g0118071 32.456 114 66 3 106 216 41 146 8.53e-14 67.8
Msa1330050 MtrunA17_Chr3g0120011 38.961 77 45 1 142 216 85 161 1.02e-13 67.4
Msa1330050 MtrunA17_Chr5g0430781 40.000 80 44 2 139 216 75 152 1.40e-13 67.4
Msa1330050 MtrunA17_Chr3g0143301 46.667 75 38 2 145 219 44 116 1.64e-13 65.1
Msa1330050 MtrunA17_Chr7g0274721 36.620 71 45 0 146 216 65 135 1.69e-13 66.2
Msa1330050 MtrunA17_Chr8g0383871 41.667 72 40 1 148 217 111 182 2.35e-13 66.6
Msa1330050 MtrunA17_Chr8g0383921 41.667 72 40 1 148 217 110 181 2.45e-13 66.6
Msa1330050 MtrunA17_Chr7g0244851 41.892 74 38 2 149 219 81 152 4.30e-13 65.1
Msa1330050 MtrunA17_Chr8g0370171 41.333 75 41 1 145 216 66 140 4.98e-13 65.5
Msa1330050 MtrunA17_Chr4g0030831 40.541 74 41 1 149 219 84 157 5.70e-13 64.7
Msa1330050 MtrunA17_Chr6g0486651 43.836 73 38 2 149 219 93 164 5.98e-13 67.0
Msa1330050 MtrunA17_Chr2g0327791 44.118 68 35 1 155 219 113 180 6.17e-13 65.5
Msa1330050 MtrunA17_Chr5g0407431 42.466 73 39 1 148 217 76 148 6.19e-13 65.5
Msa1330050 MtrunA17_Chr8g0383931 50.000 52 26 0 166 217 133 184 7.37e-13 65.5
Msa1330050 MtrunA17_Chr8g0383901 50.000 52 26 0 166 217 123 174 7.41e-13 65.1
Msa1330050 MtrunA17_Chr6g0451041 55.319 47 21 0 173 219 17 63 7.65e-13 62.4
Msa1330050 MtrunA17_Chr7g0248831 38.356 73 42 1 149 218 80 152 9.64e-13 64.3
Msa1330050 MtrunA17_Chr8g0348151 40.000 70 41 1 149 218 90 158 1.15e-12 64.3
Msa1330050 MtrunA17_Chr3g0086801 41.333 75 43 1 145 219 76 149 1.20e-12 63.5
Msa1330050 MtrunA17_Chr2g0295571 39.726 73 43 1 148 219 92 164 1.49e-12 65.5
Msa1330050 MtrunA17_Chr1g0157091 45.070 71 37 2 149 219 48 116 1.57e-12 62.4
Msa1330050 MtrunA17_Chr7g0248841 51.064 47 23 0 171 217 101 147 1.58e-12 63.2
Msa1330050 MtrunA17_Chr5g0426871 38.667 75 42 3 149 219 108 182 2.10e-12 65.5
Msa1330050 MtrunA17_Chr3g0113811 42.105 76 39 2 146 216 73 148 2.33e-12 63.2
Msa1330050 MtrunA17_Chr4g0067591 37.662 77 47 1 143 219 74 149 2.85e-12 64.3
Msa1330050 MtrunA17_Chr3g0118061 31.250 112 67 4 115 216 35 146 3.17e-12 63.5
Msa1330050 MtrunA17_Chr3g0129671 40.845 71 40 1 149 217 109 179 3.38e-12 63.5
Msa1330050 MtrunA17_Chr1g0184631 38.158 76 43 2 146 217 65 140 3.61e-12 62.8
Msa1330050 MtrunA17_Chr7g0274711 38.158 76 43 2 146 217 64 139 3.81e-12 62.4
Msa1330050 MtrunA17_Chr3g0105441 36.364 77 44 1 148 219 101 177 4.03e-12 63.9
Msa1330050 MtrunA17_Chr2g0307411 40.278 72 41 1 148 219 76 145 5.01e-12 62.4
Msa1330050 MtrunA17_Chr1g0167811 39.241 79 38 3 148 219 93 168 5.34e-12 63.9
Msa1330050 MtrunA17_Chr1g0191601 42.373 59 30 1 161 219 118 172 5.67e-12 63.9
Msa1330050 MtrunA17_Chr7g0274741 38.356 73 41 2 148 216 69 141 6.49e-12 62.0
Msa1330050 MtrunA17_Chr5g0441191 35.714 70 44 1 147 216 173 241 7.33e-12 63.5
Msa1330050 MtrunA17_Chr7g0274731 35.211 71 46 0 146 216 86 156 7.53e-12 62.0
Msa1330050 MtrunA17_Chr2g0325141 50.000 46 23 0 174 219 91 136 7.76e-12 60.8
Msa1330050 MtrunA17_Chr7g0273051 47.826 46 24 0 174 219 120 165 9.71e-12 63.5
Msa1330050 MtrunA17_Chr3g0132491 39.474 76 42 2 148 219 106 181 1.11e-11 63.2
Msa1330050 MtrunA17_Chr4g0013561 35.294 68 44 0 149 216 85 152 1.16e-11 62.0
Msa1330050 MtrunA17_Chr5g0409211 41.791 67 33 1 156 216 70 136 1.17e-11 61.2
Msa1330050 MtrunA17_Chr7g0276591 38.710 62 38 0 155 216 82 143 1.18e-11 62.8
Msa1330050 MtrunA17_Chr3g0144241 38.571 70 43 0 148 217 78 147 1.20e-11 61.6
Msa1330050 MtrunA17_Chr1g0198201 38.028 71 42 1 148 216 60 130 1.25e-11 62.0
Msa1330050 MtrunA17_Chr8g0345361 43.056 72 39 2 149 219 92 162 1.30e-11 62.4
Msa1330050 MtrunA17_Chr4g0073131 42.424 66 37 1 155 219 82 147 1.46e-11 60.8
Msa1330050 MtrunA17_Chr1g0184171 37.333 75 43 2 148 219 102 175 1.48e-11 62.8
Msa1330050 MtrunA17_Chr8g0366561 50.000 48 24 0 172 219 140 187 1.51e-11 62.8
Msa1330050 MtrunA17_Chr1g0171791 36.986 73 45 1 147 219 132 203 1.65e-11 62.0
Msa1330050 MtrunA17_Chr5g0409201 40.000 70 36 1 155 218 68 137 2.00e-11 60.1
Msa1330050 MtrunA17_Chr3g0117821 51.163 43 21 0 174 216 85 127 2.04e-11 60.1
Msa1330050 MtrunA17_Chr8g0388201 29.167 96 65 1 125 217 295 390 2.35e-11 62.4
Msa1330050 MtrunA17_Chr1g0169651 39.437 71 40 2 149 219 660 727 2.75e-11 62.4
Msa1330050 MtrunA17_Chr8g0369571 48.889 45 23 0 174 218 90 134 2.76e-11 60.1
Msa1330050 MtrunA17_Chr3g0126611 32.184 87 55 2 133 216 288 373 2.88e-11 62.0
Msa1330050 MtrunA17_Chr4g0073141 32.632 95 53 2 136 219 95 189 3.25e-11 62.0
Msa1330050 MtrunA17_Chr4g0027761 39.130 69 40 1 148 216 99 165 3.34e-11 60.5
Msa1330050 MtrunA17_Chr4g0052671 50.000 44 22 0 174 217 106 149 3.39e-11 59.3
Msa1330050 MtrunA17_Chr6g0470751 36.842 76 44 2 148 219 96 171 3.92e-11 61.2
Msa1330050 MtrunA17_Chr2g0325161 50.000 46 23 0 174 219 123 168 3.97e-11 60.8
Msa1330050 MtrunA17_Chr3g0133001 52.174 46 22 0 174 219 156 201 4.12e-11 61.6
Msa1330050 MtrunA17_Chr8g0350741 51.111 45 22 0 172 216 87 131 4.21e-11 59.7
Msa1330050 MtrunA17_Chr2g0279431 48.936 47 24 0 170 216 157 203 4.41e-11 61.6
Msa1330050 MtrunA17_Chr2g0300331 48.077 52 27 0 168 219 163 214 4.55e-11 60.5
Msa1330050 MtrunA17_Chr5g0427431 52.174 46 22 0 174 219 160 205 4.88e-11 61.6
Msa1330050 MtrunA17_Chr3g0126931 28.571 147 86 4 89 216 23 169 4.89e-11 61.2
Msa1330050 MtrunA17_Chr2g0297011 34.722 72 46 1 149 219 76 147 6.93e-11 58.9
Msa1330050 MtrunA17_Chr5g0396601 38.889 72 41 2 149 217 322 393 9.58e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330050 AT1G74410.1 56.373 204 84 2 17 219 22 221 1.26e-72 220
Msa1330050 AT5G66070.5 41.063 207 116 4 14 219 20 221 3.47e-49 160
Msa1330050 AT5G66070.1 41.063 207 116 4 14 219 20 221 3.47e-49 160
Msa1330050 AT5G66070.2 37.719 228 118 4 14 219 20 245 8.94e-47 155
Msa1330050 AT2G17730.1 38.462 208 120 4 16 219 38 241 1.33e-45 152
Msa1330050 AT4G35840.1 36.667 210 120 3 19 219 31 236 7.86e-44 147
Msa1330050 AT2G17730.2 38.191 199 115 4 16 210 38 232 2.13e-40 139
Msa1330050 AT4G35840.2 31.897 232 116 3 25 219 42 268 4.48e-37 130
Msa1330050 AT3G20395.1 29.825 228 134 5 5 219 2 216 1.26e-29 110
Msa1330050 AT5G66070.4 36.527 167 100 3 14 179 20 181 1.11e-26 101
Msa1330050 AT5G66070.3 33.511 188 101 4 14 179 20 205 2.28e-24 96.3
Msa1330050 AT3G10910.1 40.816 98 49 3 125 219 68 159 1.59e-16 74.7
Msa1330050 AT5G05280.1 41.667 72 42 0 148 219 87 158 1.66e-15 72.0
Msa1330050 AT2G27940.1 42.254 71 41 0 148 218 114 184 3.31e-15 72.4
Msa1330050 AT2G17450.1 44.872 78 35 2 148 217 68 145 1.97e-14 69.3
Msa1330050 AT5G01880.1 44.444 72 39 1 149 219 79 150 2.54e-14 68.6
Msa1330050 AT3G16720.1 38.961 77 47 0 143 219 88 164 6.62e-14 69.7
Msa1330050 AT4G10160.1 39.706 68 41 0 149 216 72 139 1.68e-13 67.4
Msa1330050 AT5G53110.1 37.363 91 51 2 131 218 287 374 2.02e-13 68.9
Msa1330050 AT5G42200.1 42.466 73 41 1 145 216 74 146 2.40e-13 65.9
Msa1330050 AT4G10150.1 38.235 68 42 0 149 216 86 153 4.62e-13 66.6
Msa1330050 AT4G17905.2 42.857 63 34 1 157 219 166 226 8.97e-13 66.6
Msa1330050 AT4G17905.1 42.857 63 34 1 157 219 140 200 9.01e-13 66.6
Msa1330050 AT4G09120.1 37.374 99 51 4 125 219 77 168 9.98e-13 66.6
Msa1330050 AT2G46160.1 39.241 79 39 1 149 218 104 182 1.13e-12 65.1
Msa1330050 AT2G46160.2 39.241 79 39 1 149 218 104 182 1.13e-12 65.1
Msa1330050 AT5G57750.1 37.879 66 39 1 155 218 101 166 1.16e-12 65.1
Msa1330050 AT1G20823.1 41.667 72 40 1 148 217 83 154 1.23e-12 64.7
Msa1330050 AT1G76410.1 35.165 91 57 1 129 217 57 147 1.63e-12 63.9
Msa1330050 AT1G35330.1 42.466 73 38 2 148 216 99 171 2.88e-12 65.1
Msa1330050 AT2G35910.1 40.580 69 40 1 149 216 121 189 3.48e-12 63.9
Msa1330050 AT2G42360.1 41.892 74 41 1 148 219 80 153 4.00e-12 63.9
Msa1330050 AT3G14320.1 52.174 46 22 0 174 219 88 133 4.90e-12 63.2
Msa1330050 AT4G40070.1 47.059 51 27 0 169 219 119 169 5.11e-12 64.3
Msa1330050 AT3G18930.2 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1330050 AT3G18930.3 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1330050 AT3G18930.1 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1330050 AT4G09130.1 38.710 93 50 4 131 219 76 165 5.84e-12 64.3
Msa1330050 AT3G18773.1 38.889 72 42 1 148 217 102 173 7.23e-12 62.8
Msa1330050 AT2G20030.1 39.726 73 41 2 149 219 98 169 7.47e-12 64.3
Msa1330050 AT5G10380.1 41.667 72 41 1 149 219 109 180 8.35e-12 63.5
Msa1330050 AT1G53820.1 50.000 46 23 0 174 219 120 165 9.22e-12 63.5
Msa1330050 AT1G49220.1 40.541 74 42 1 146 217 105 178 9.86e-12 63.2
Msa1330050 AT1G72310.1 37.333 75 47 0 145 219 98 172 1.11e-11 63.5
Msa1330050 AT2G42350.1 42.029 69 40 0 148 216 74 142 1.39e-11 62.0
Msa1330050 AT3G05200.1 52.174 46 22 0 174 219 128 173 1.48e-11 63.5
Msa1330050 AT4G30370.1 40.541 74 39 2 149 219 89 160 1.67e-11 61.2
Msa1330050 AT4G09100.1 34.615 78 47 1 146 219 53 130 1.82e-11 60.1
Msa1330050 AT4G28890.1 39.726 73 41 2 149 219 97 168 2.19e-11 62.8
Msa1330050 AT2G18670.1 39.726 73 42 1 149 219 83 155 2.36e-11 60.8
Msa1330050 AT2G40830.1 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1330050 AT2G40830.3 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1330050 AT2G40830.4 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1330050 AT2G40830.2 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1330050 AT1G33480.1 35.294 68 44 0 149 216 75 142 2.86e-11 61.6
Msa1330050 AT1G35625.1 38.889 72 40 3 150 218 207 277 3.32e-11 62.0
Msa1330050 AT1G28040.1 30.973 113 66 3 106 218 197 297 3.99e-11 61.6
Msa1330050 AT2G18650.1 35.616 73 45 1 149 219 99 171 4.00e-11 62.0
Msa1330050 AT3G48030.1 36.986 73 44 2 149 219 180 252 4.77e-11 61.6
Msa1330050 AT2G44578.1 42.308 52 30 0 168 219 64 115 4.77e-11 59.3
Msa1330050 AT4G30400.1 37.143 70 42 1 149 216 107 176 5.44e-11 62.0
Msa1330050 AT3G48030.2 36.986 73 44 2 149 219 102 174 5.56e-11 61.2
Msa1330050 AT1G53010.1 30.233 129 68 4 112 219 48 175 6.89e-11 59.3
Msa1330050 AT1G49200.1 38.961 77 39 2 146 217 106 179 7.58e-11 60.1
Msa1330050 AT5G17600.1 48.837 43 22 0 174 216 142 184 7.78e-11 61.2
Msa1330050 AT1G35630.1 41.667 72 38 3 150 218 207 277 9.07e-11 60.8
Msa1330050 AT2G25410.1 46.000 50 27 0 169 218 322 371 9.45e-11 60.8
Msa1330050 AT5G07040.1 35.616 73 44 1 149 218 66 138 9.91e-11 58.5

Find 78 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACCAAAATAGCATTAT+TGG 0.170704 8_4:-49083118 None:intergenic
GTTTCATTGGGTGCCATTGC+CGG 0.215022 8_4:+49081249 Msa1330050:CDS
CTAGTCCAATAATGCTATTT+TGG 0.245191 8_4:+49083113 Msa1330050:three_prime_UTR
ATTGTAGCTGGGGCCCTTAC+AGG 0.247414 8_4:+49081171 Msa1330050:intron
CAGCAGCTTATGATTGAAAA+TGG 0.286404 8_4:-49080381 None:intergenic
ACGAAACGAAATTCCCTTCT+TGG 0.308335 8_4:+49080472 Msa1330050:CDS
TGGAAGCTTCCCGTGCTTAT+TGG 0.315076 8_4:+49081295 Msa1330050:CDS
AAAGGCGCATAATAGAAATT+TGG 0.316639 8_4:-49080510 None:intergenic
AAAATTTGAAATAGCAAATC+AGG 0.318338 8_4:+49083181 Msa1330050:three_prime_UTR
TTTCATTGGGTGCCATTGCC+GGG 0.321224 8_4:+49081250 Msa1330050:CDS
TTCACGCGGCGCGTCTTCTA+TGG 0.328316 8_4:+49081335 Msa1330050:CDS
CTCTGATTGGCTATCATTCA+TGG 0.356125 8_4:+49080439 Msa1330050:exon
TTCCATTGATTGATTGTTGA+CGG 0.369847 8_4:+49080308 Msa1330050:five_prime_UTR
CTGTGATATAATGATCATAA+AGG 0.370931 8_4:-49080253 None:intergenic
CGAAACGAAATTCCCTTCTT+GGG 0.382549 8_4:+49080473 Msa1330050:CDS
TTTCTGTGCAATATGTTTGC+AGG 0.383281 8_4:+49082667 Msa1330050:CDS
TCCCCGGAGAAAACCACTTT+TGG 0.384936 8_4:-49081226 None:intergenic
TACACATTCTGTCTGCAAAT+AGG 0.395070 8_4:-49082943 None:intergenic
AATTTACAGGACATTGAAGT+TGG 0.400528 8_4:+49082835 Msa1330050:intron
TTTCTCCGGGGAGTTTCATT+GGG 0.418806 8_4:+49081237 Msa1330050:CDS
AGCTGCTGTTGCTGCACCTA+TGG 0.420837 8_4:+49080396 Msa1330050:five_prime_UTR
ACTTGATATGCGTGGACAAA+TGG 0.421325 8_4:+49082899 Msa1330050:CDS
AAAACATGATAACTGAGTAT+TGG 0.436148 8_4:-49083144 None:intergenic
CACCAGCTATAGCTCCTGTA+AGG 0.439321 8_4:-49081185 None:intergenic
TGAATCCCGTGACAATCCTC+TGG 0.451565 8_4:-49082591 None:intergenic
GAATAAGCTACCGCACCATA+TGG 0.453688 8_4:+49082616 Msa1330050:CDS
TGGCTATCATTCATGGAAAC+TGG 0.457921 8_4:+49080446 Msa1330050:exon
TTCCCGTGCTTATTGGTGTA+TGG 0.470182 8_4:+49081302 Msa1330050:CDS
ACGTTTCCACACTCTCTGAT+TGG 0.472939 8_4:+49080426 Msa1330050:five_prime_UTR
CGCATATCAAGTGAAAAGTA+TGG 0.479146 8_4:-49082888 None:intergenic
CATTGCCGGGGCTATACTCT+CGG 0.488812 8_4:+49081263 Msa1330050:CDS
TCATGCCCTTCGAAACCATA+TGG 0.493143 8_4:-49082631 None:intergenic
GCAGCTAAGGCTACCAAAAG+TGG 0.501253 8_4:+49081213 Msa1330050:CDS
GCCCTTACAGGAGCTATAGC+TGG 0.509484 8_4:+49081183 Msa1330050:CDS
ATTTCTCCATGAAGTGGGAG+GGG 0.523573 8_4:+49083047 Msa1330050:three_prime_UTR
TTTCTCCATGAAGTGGGAGG+GGG 0.525103 8_4:+49083048 Msa1330050:three_prime_UTR
TATACTCTCGGTGGAGGTGT+TGG 0.526459 8_4:+49081275 Msa1330050:CDS
TTGTAAGCAGTTAAAATAGC+AGG 0.527550 8_4:-49081623 None:intergenic
TAGAGGAACTTGTTCGTGGA+AGG 0.529453 8_4:+49081579 Msa1330050:CDS
TGCAGTGAGAGTTCCAGAAA+AGG 0.530821 8_4:-49080528 None:intergenic
AGCCGTCAACAATCAATCAA+TGG 0.532462 8_4:-49080310 None:intergenic
TTCATAGAGGAACTTGTTCG+TGG 0.535580 8_4:+49081575 Msa1330050:CDS
CTCCACCGAGAGTATAGCCC+CGG 0.540703 8_4:-49081268 None:intergenic
TCAATGGAAAATGAAAAGTA+AGG 0.543772 8_4:-49080294 None:intergenic
GACAAAACGAAAACCCAAGA+AGG 0.544046 8_4:-49080486 None:intergenic
TTATTTCTCCATGAAGTGGG+AGG 0.545078 8_4:+49083045 Msa1330050:three_prime_UTR
TATTGGTGTATGGAACAAAC+TGG 0.545201 8_4:+49081312 Msa1330050:CDS
ACCGCACCATATGGTTTCGA+AGG 0.553055 8_4:+49082625 Msa1330050:CDS
TATTTCTCCATGAAGTGGGA+GGG 0.558759 8_4:+49083046 Msa1330050:three_prime_UTR
TTCATTGGGTGCCATTGCCG+GGG 0.560934 8_4:+49081251 Msa1330050:CDS
TGGAAACGTTTATGAGCCAT+AGG 0.562502 8_4:-49080412 None:intergenic
CGAGAGTATAGCCCCGGCAA+TGG 0.563534 8_4:-49081262 None:intergenic
TTCCATACACCAATAAGCAC+GGG 0.567244 8_4:-49081304 None:intergenic
TTCGAAGGGCATGAAGGCAG+AGG 0.573221 8_4:+49082640 Msa1330050:CDS
TGATTGAAAATGGAAGGATA+AGG 0.574012 8_4:-49080371 None:intergenic
AGCTTATGATTGAAAATGGA+AGG 0.578222 8_4:-49080377 None:intergenic
AATCCAGGCAGATTTCATAG+AGG 0.579193 8_4:+49081562 Msa1330050:intron
GAAAATGAAAAGTAAGGTGT+TGG 0.587260 8_4:-49080288 None:intergenic
TGATAGCCAATCAGAGAGTG+TGG 0.589978 8_4:-49080432 None:intergenic
GTTCCATACACCAATAAGCA+CGG 0.597188 8_4:-49081305 None:intergenic
TATTGGACTAGATTACAGAA+TGG 0.600503 8_4:-49083101 None:intergenic
CGGGGCTATACTCTCGGTGG+AGG 0.602147 8_4:+49081269 Msa1330050:CDS
AAGGATAAGGAAGATGAAGC+TGG 0.608532 8_4:-49080358 None:intergenic
TATGGTTTCGAAGGGCATGA+AGG 0.614703 8_4:+49082634 Msa1330050:CDS
ACAAAACGAAAACCCAAGAA+GGG 0.623335 8_4:-49080485 None:intergenic
TGGCACCCAATGAAACTCCC+CGG 0.623545 8_4:-49081242 None:intergenic
CTATCATTCATGGAAACTGG+TGG 0.623614 8_4:+49080449 Msa1330050:exon
GTTGGAGTTGCTAATAACGC+TGG 0.623875 8_4:+49082530 Msa1330050:CDS
GAATCCCGTGACAATCCTCT+GGG 0.625719 8_4:-49082590 None:intergenic
ATCACCCAGAGGATTGTCAC+GGG 0.633383 8_4:+49082586 Msa1330050:CDS
ACCAGCTATAGCTCCTGTAA+GGG 0.637027 8_4:-49081184 None:intergenic
CCGCACCATATGGTTTCGAA+GGG 0.637296 8_4:+49082626 Msa1330050:CDS
CCCTTCGAAACCATATGGTG+CGG 0.643401 8_4:-49082626 None:intergenic
TATCACCCAGAGGATTGTCA+CGG 0.646030 8_4:+49082585 Msa1330050:CDS
TGCCGGGGCTATACTCTCGG+TGG 0.658573 8_4:+49081266 Msa1330050:CDS
ATGGAACAAACTGGTTCACG+CGG 0.720989 8_4:+49081321 Msa1330050:CDS
CATAGCTTAGTATCACCCAG+AGG 0.736019 8_4:+49082575 Msa1330050:CDS
TGAAAAGTATGGTCGCAACG+AGG 0.774791 8_4:-49082877 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAAAACAAATTATAAA+TGG - chr8_4:49082319-49082338 None:intergenic 10.0%
!!! TTATTTTGTGTTAATATTTA+CGG + chr8_4:49082975-49082994 Msa1330050:three_prime_UTR 10.0%
!!! TTTTTTTGTTTGATTTGAAT+TGG + chr8_4:49080679-49080698 Msa1330050:intron 15.0%
!!! TATGAAAGTTTAAGAATTTT+TGG + chr8_4:49080758-49080777 Msa1330050:intron 15.0%
!!! ATGAAAGTTTAAGAATTTTT+GGG + chr8_4:49080759-49080778 Msa1330050:intron 15.0%
!! TCAGAATTTAAATATGATAT+AGG - chr8_4:49082040-49082059 None:intergenic 15.0%
!!! TATTTTGTGTTAATATTTAC+GGG + chr8_4:49082976-49082995 Msa1330050:three_prime_UTR 15.0%
!!! TGAAAGTTTAAGAATTTTTG+GGG + chr8_4:49080760-49080779 Msa1330050:intron 20.0%
!!! ATAATTTGTTTATCCAAACA+GGG - chr8_4:49080825-49080844 None:intergenic 20.0%
!!! AATAATTTGTTTATCCAAAC+AGG - chr8_4:49080826-49080845 None:intergenic 20.0%
!! TCAAATAAACTAATTCAAAC+AGG + chr8_4:49081083-49081102 Msa1330050:intron 20.0%
!!! ATTGGTTTAAAATTATGCAT+CGG - chr8_4:49081529-49081548 None:intergenic 20.0%
!!! AAATTCTGAAACTAAAAAAG+AGG + chr8_4:49082051-49082070 Msa1330050:intron 20.0%
!! TACTATGTGTTAAATTTGTA+TGG - chr8_4:49082165-49082184 None:intergenic 20.0%
!! ATACAAATTTAACACATAGT+AGG + chr8_4:49082164-49082183 Msa1330050:intron 20.0%
!! ATTGAGTTATGTATATACAT+TGG + chr8_4:49082230-49082249 Msa1330050:intron 20.0%
!! CATTGAGAAATATAATTTAC+AGG + chr8_4:49082822-49082841 Msa1330050:intron 20.0%
!!! AAAATTTGAAATAGCAAATC+AGG + chr8_4:49083181-49083200 Msa1330050:three_prime_UTR 20.0%
! CTGTGATATAATGATCATAA+AGG - chr8_4:49080256-49080275 None:intergenic 25.0%
! TCAATGGAAAATGAAAAGTA+AGG - chr8_4:49080297-49080316 None:intergenic 25.0%
! TTGCACAAATCAATTTATTC+TGG + chr8_4:49080646-49080665 Msa1330050:intron 25.0%
!! TAAAAGAATTGCTATTGACT+AGG - chr8_4:49080799-49080818 None:intergenic 25.0%
!! TAGTCAATAGCAATTCTTTT+AGG + chr8_4:49080798-49080817 Msa1330050:intron 25.0%
!! AGTCAATAGCAATTCTTTTA+GGG + chr8_4:49080799-49080818 Msa1330050:intron 25.0%
! GTTTATCATATAAGCACTTA+CGG + chr8_4:49080867-49080886 Msa1330050:intron 25.0%
! TTTATCATATAAGCACTTAC+GGG + chr8_4:49080868-49080887 Msa1330050:intron 25.0%
! GTTTATTTGAGCTTATCTAT+TGG - chr8_4:49081073-49081092 None:intergenic 25.0%
! AGCTTGAAATGCATATATAT+AGG + chr8_4:49081422-49081441 Msa1330050:intron 25.0%
! GGATTTAAATGTTACAGAAT+TGG - chr8_4:49081547-49081566 None:intergenic 25.0%
! ATTCTGTAACATTTAAATCC+AGG + chr8_4:49081547-49081566 Msa1330050:intron 25.0%
!!! TTTTAAAAGGCAAAAATTGG+GGG - chr8_4:49081679-49081698 None:intergenic 25.0%
!!! CTTTTAAAAGGCAAAAATTG+GGG - chr8_4:49081680-49081699 None:intergenic 25.0%
!!! GCTTTTAAAAGGCAAAAATT+GGG - chr8_4:49081681-49081700 None:intergenic 25.0%
!!! TGCTTTTAAAAGGCAAAAAT+TGG - chr8_4:49081682-49081701 None:intergenic 25.0%
!!! TAGTTTTATGAGCATCTTTT+GGG + chr8_4:49081907-49081926 Msa1330050:intron 25.0%
! ATCTTTAAATGACCTACATT+GGG - chr8_4:49082402-49082421 None:intergenic 25.0%
! AATCTTTAAATGACCTACAT+TGG - chr8_4:49082403-49082422 None:intergenic 25.0%
! TTTCAGGATTTGTGATTTAA+TGG + chr8_4:49082700-49082719 Msa1330050:intron 25.0%
! ATAGGGAAAATGAAAAATTG+TGG - chr8_4:49082759-49082778 None:intergenic 25.0%
! TATCATCCAAATCTCATATA+GGG - chr8_4:49082776-49082795 None:intergenic 25.0%
! ATATCATCCAAATCTCATAT+AGG - chr8_4:49082777-49082796 None:intergenic 25.0%
!!! TTTTTTATTTCTCCATGAAG+TGG + chr8_4:49083041-49083060 Msa1330050:three_prime_UTR 25.0%
!!! TTTTTATTTCTCCATGAAGT+GGG + chr8_4:49083042-49083061 Msa1330050:three_prime_UTR 25.0%
! TTCTACCAAAATAGCATTAT+TGG - chr8_4:49083121-49083140 None:intergenic 25.0%
! AAAACATGATAACTGAGTAT+TGG - chr8_4:49083147-49083166 None:intergenic 25.0%
GAAAATGAAAAGTAAGGTGT+TGG - chr8_4:49080291-49080310 None:intergenic 30.0%
! TTCCATTGATTGATTGTTGA+CGG + chr8_4:49080308-49080327 Msa1330050:five_prime_UTR 30.0%
TGATTGAAAATGGAAGGATA+AGG - chr8_4:49080374-49080393 None:intergenic 30.0%
AGCTTATGATTGAAAATGGA+AGG - chr8_4:49080380-49080399 None:intergenic 30.0%
! AAAGGCGCATAATAGAAATT+TGG - chr8_4:49080513-49080532 None:intergenic 30.0%
AGGATATTAGAGATTACCAA+AGG - chr8_4:49080561-49080580 None:intergenic 30.0%
AAATCATCATAGATGGTACA+AGG - chr8_4:49080581-49080600 None:intergenic 30.0%
TTGTACCATCTATGATGATT+TGG + chr8_4:49080580-49080599 Msa1330050:intron 30.0%
AATTACGAAGCTTAAACATG+AGG + chr8_4:49080613-49080632 Msa1330050:intron 30.0%
ATTACGAAGCTTAAACATGA+GGG + chr8_4:49080614-49080633 Msa1330050:intron 30.0%
ATTGGTTGTAGTGAAATTCA+TGG + chr8_4:49080697-49080716 Msa1330050:intron 30.0%
TTGTAGTGAAATTCATGGTT+TGG + chr8_4:49080702-49080721 Msa1330050:intron 30.0%
!!! TTTAAGAATTTTTGGGGTGT+TGG + chr8_4:49080766-49080785 Msa1330050:intron 30.0%
!! GTTATTTCTATAAGCTCTTC+AGG - chr8_4:49080971-49080990 None:intergenic 30.0%
AAACTTATGAAAACAGCTTC+CGG - chr8_4:49081016-49081035 None:intergenic 30.0%
!! TTGTGTTTTTCAATTGTAGC+TGG + chr8_4:49081159-49081178 Msa1330050:intron 30.0%
!! TGTGTTTTTCAATTGTAGCT+GGG + chr8_4:49081160-49081179 Msa1330050:intron 30.0%
! TTCACCTTGATTTTTGTATG+TGG + chr8_4:49081387-49081406 Msa1330050:intron 30.0%
TTGTCCACATACAAAAATCA+AGG - chr8_4:49081394-49081413 None:intergenic 30.0%
TAAGATGCATCTTAAAAACC+AGG - chr8_4:49081480-49081499 None:intergenic 30.0%
!!! TTAAGAGTGTGTTTTTTGAG+TGG + chr8_4:49081497-49081516 Msa1330050:intron 30.0%
TTAAAATTATGCATCGGAGA+TGG - chr8_4:49081523-49081542 None:intergenic 30.0%
TTGTAAGCAGTTAAAATAGC+AGG - chr8_4:49081626-49081645 None:intergenic 30.0%
!! ACAAAGCTACTGCTTTTAAA+AGG - chr8_4:49081692-49081711 None:intergenic 30.0%
ATATAATAGTGTAGTTGCGA+TGG + chr8_4:49081733-49081752 Msa1330050:intron 30.0%
! ACGGAAAATTTGCATTTTTC+AGG - chr8_4:49081874-49081893 None:intergenic 30.0%
!!! CTAGTTTTATGAGCATCTTT+TGG + chr8_4:49081906-49081925 Msa1330050:intron 30.0%
! GAGGAGAAAAAAACATTTTC+AGG - chr8_4:49081970-49081989 None:intergenic 30.0%
TGATTACATGAATCTCTTTC+TGG + chr8_4:49082352-49082371 Msa1330050:intron 30.0%
TCTTTAAATGACCTACATTG+GGG - chr8_4:49082401-49082420 None:intergenic 30.0%
!!! TCATTTGTTTTAGTTTGAGC+AGG + chr8_4:49082508-49082527 Msa1330050:intron 30.0%
! CATTTTCCCTATATGAGATT+TGG + chr8_4:49082767-49082786 Msa1330050:intron 30.0%
AATTTACAGGACATTGAAGT+TGG + chr8_4:49082835-49082854 Msa1330050:intron 30.0%
! TATTGGACTAGATTACAGAA+TGG - chr8_4:49083104-49083123 None:intergenic 30.0%
!! CTAGTCCAATAATGCTATTT+TGG + chr8_4:49083113-49083132 Msa1330050:three_prime_UTR 30.0%
! ATGAAGCTGGCTAGATTTTT+GGG - chr8_4:49080348-49080367 None:intergenic 35.0%
CAGCAGCTTATGATTGAAAA+TGG - chr8_4:49080384-49080403 None:intergenic 35.0%
ACAAAACGAAAACCCAAGAA+GGG - chr8_4:49080488-49080507 None:intergenic 35.0%
! TTTCTATTATGCGCCTTTTC+TGG + chr8_4:49080515-49080534 Msa1330050:CDS 35.0%
AACAGCCAAATCATCATAGA+TGG - chr8_4:49080588-49080607 None:intergenic 35.0%
!!! TTTTTGTTGGCTTCTTCTCA+TGG + chr8_4:49081135-49081154 Msa1330050:intron 35.0%
!! GTGTTTTTCAATTGTAGCTG+GGG + chr8_4:49081161-49081180 Msa1330050:intron 35.0%
TATTGGTGTATGGAACAAAC+TGG + chr8_4:49081312-49081331 Msa1330050:CDS 35.0%
TCTATGGTAAGTGATCGTTA+TGG + chr8_4:49081351-49081370 Msa1330050:intron 35.0%
TCATAAAACTAGAGAAGAGC+GGG - chr8_4:49081898-49081917 None:intergenic 35.0%
CTCATAAAACTAGAGAAGAG+CGG - chr8_4:49081899-49081918 None:intergenic 35.0%
AGGTGCAATGAAAGAAAATG+AGG - chr8_4:49081950-49081969 None:intergenic 35.0%
!!! TTGTTTTAGTTTGAGCAGGT+TGG + chr8_4:49082512-49082531 Msa1330050:intron 35.0%
TTTCTGTGCAATATGTTTGC+AGG + chr8_4:49082667-49082686 Msa1330050:CDS 35.0%
! TGCAGGTGATTATTCTTTTC+AGG + chr8_4:49082684-49082703 Msa1330050:intron 35.0%
CGCATATCAAGTGAAAAGTA+TGG - chr8_4:49082891-49082910 None:intergenic 35.0%
! TACTTTTCACTTGATATGCG+TGG + chr8_4:49082891-49082910 Msa1330050:CDS 35.0%
TACACATTCTGTCTGCAAAT+AGG - chr8_4:49082946-49082965 None:intergenic 35.0%
AGCCGTCAACAATCAATCAA+TGG - chr8_4:49080313-49080332 None:intergenic 40.0%
! TGAAGCTGGCTAGATTTTTG+GGG - chr8_4:49080347-49080366 None:intergenic 40.0%
! GATGAAGCTGGCTAGATTTT+TGG - chr8_4:49080349-49080368 None:intergenic 40.0%
AAGGATAAGGAAGATGAAGC+TGG - chr8_4:49080361-49080380 None:intergenic 40.0%
TGGAAACGTTTATGAGCCAT+AGG - chr8_4:49080415-49080434 None:intergenic 40.0%
! CTCTGATTGGCTATCATTCA+TGG + chr8_4:49080439-49080458 Msa1330050:exon 40.0%
TGGCTATCATTCATGGAAAC+TGG + chr8_4:49080446-49080465 Msa1330050:exon 40.0%
CTATCATTCATGGAAACTGG+TGG + chr8_4:49080449-49080468 Msa1330050:exon 40.0%
ACGAAACGAAATTCCCTTCT+TGG + chr8_4:49080472-49080491 Msa1330050:CDS 40.0%
CGAAACGAAATTCCCTTCTT+GGG + chr8_4:49080473-49080492 Msa1330050:CDS 40.0%
GACAAAACGAAAACCCAAGA+AGG - chr8_4:49080489-49080508 None:intergenic 40.0%
!!! AATTCTTTTAGGGCCCTGTT+TGG + chr8_4:49080809-49080828 Msa1330050:intron 40.0%
GAAACAGCTAATGAACATGC+CGG + chr8_4:49080994-49081013 Msa1330050:intron 40.0%
TCAAAGCAACACTTAGGTCA+GGG - chr8_4:49081109-49081128 None:intergenic 40.0%
!!! GCTTTGAGGTGTGTTTTTGT+TGG + chr8_4:49081122-49081141 Msa1330050:intron 40.0%
! CTACCAAAAGTGGTTTTCTC+CGG + chr8_4:49081223-49081242 Msa1330050:CDS 40.0%
! TACCAAAAGTGGTTTTCTCC+GGG + chr8_4:49081224-49081243 Msa1330050:CDS 40.0%
TTCCATACACCAATAAGCAC+GGG - chr8_4:49081307-49081326 None:intergenic 40.0%
GTTCCATACACCAATAAGCA+CGG - chr8_4:49081308-49081327 None:intergenic 40.0%
GCAGCACAAATCACAATACA+AGG - chr8_4:49081458-49081477 None:intergenic 40.0%
AATCCAGGCAGATTTCATAG+AGG + chr8_4:49081562-49081581 Msa1330050:intron 40.0%
! TTCATAGAGGAACTTGTTCG+TGG + chr8_4:49081575-49081594 Msa1330050:CDS 40.0%
TGACAGTATCAAGAGTCGTT+TGG + chr8_4:49081768-49081787 Msa1330050:intron 40.0%
GTATCAAGAGTCGTTTGGTA+CGG + chr8_4:49081773-49081792 Msa1330050:intron 40.0%
ACCTTTGCAAAACAAGCTTC+AGG - chr8_4:49082209-49082228 None:intergenic 40.0%
GTTGCAAAAATCAACAAGGG+GGG - chr8_4:49082379-49082398 None:intergenic 40.0%
GGTTGCAAAAATCAACAAGG+GGG - chr8_4:49082380-49082399 None:intergenic 40.0%
GGGTTGCAAAAATCAACAAG+GGG - chr8_4:49082381-49082400 None:intergenic 40.0%
GGGGTTGCAAAAATCAACAA+GGG - chr8_4:49082382-49082401 None:intergenic 40.0%
TGGGGTTGCAAAAATCAACA+AGG - chr8_4:49082383-49082402 None:intergenic 40.0%
! GATTTTTGCAACCCCAATGT+AGG + chr8_4:49082387-49082406 Msa1330050:intron 40.0%
TGCTACAAACAAATCAACGC+AGG - chr8_4:49082464-49082483 None:intergenic 40.0%
ACTTGATATGCGTGGACAAA+TGG + chr8_4:49082899-49082918 Msa1330050:CDS 40.0%
! TTATTTCTCCATGAAGTGGG+AGG + chr8_4:49083045-49083064 Msa1330050:three_prime_UTR 40.0%
TATTTCTCCATGAAGTGGGA+GGG + chr8_4:49083046-49083065 Msa1330050:three_prime_UTR 40.0%
ACGTTTCCACACTCTCTGAT+TGG + chr8_4:49080426-49080445 Msa1330050:five_prime_UTR 45.0%
TGATAGCCAATCAGAGAGTG+TGG - chr8_4:49080435-49080454 None:intergenic 45.0%
TGCAGTGAGAGTTCCAGAAA+AGG - chr8_4:49080531-49080550 None:intergenic 45.0%
! CTCACTGCATGTTTTGCCTT+TGG + chr8_4:49080542-49080561 Msa1330050:CDS 45.0%
CTCAAAGCAACACTTAGGTC+AGG - chr8_4:49081110-49081129 None:intergenic 45.0%
!! CTGACCTAAGTGTTGCTTTG+AGG + chr8_4:49081108-49081127 Msa1330050:intron 45.0%
CACACCTCAAAGCAACACTT+AGG - chr8_4:49081115-49081134 None:intergenic 45.0%
ACCAGCTATAGCTCCTGTAA+GGG - chr8_4:49081187-49081206 None:intergenic 45.0%
! ACCAAAAGTGGTTTTCTCCG+GGG + chr8_4:49081225-49081244 Msa1330050:CDS 45.0%
! TTTTCTCCGGGGAGTTTCAT+TGG + chr8_4:49081236-49081255 Msa1330050:CDS 45.0%
TTTCTCCGGGGAGTTTCATT+GGG + chr8_4:49081237-49081256 Msa1330050:CDS 45.0%
TTCCCGTGCTTATTGGTGTA+TGG + chr8_4:49081302-49081321 Msa1330050:CDS 45.0%
! ATGGAACAAACTGGTTCACG+CGG + chr8_4:49081321-49081340 Msa1330050:CDS 45.0%
! GTATTGTGATTTGTGCTGCC+TGG + chr8_4:49081459-49081478 Msa1330050:intron 45.0%
GTTCCTCTATGAAATCTGCC+TGG - chr8_4:49081568-49081587 None:intergenic 45.0%
! TAGAGGAACTTGTTCGTGGA+AGG + chr8_4:49081579-49081598 Msa1330050:CDS 45.0%
TTGGGGGAGAGATATAGAGA+AGG - chr8_4:49081663-49081682 None:intergenic 45.0%
! TGGTACGGCTAATTTGTGAC+AGG + chr8_4:49081788-49081807 Msa1330050:intron 45.0%
AAACTAGAGAAGAGCGGGAA+CGG - chr8_4:49081893-49081912 None:intergenic 45.0%
CACAAGCCAATCAAACTGGA+GGG - chr8_4:49081992-49082011 None:intergenic 45.0%
TCACCACAAGCCAATCAAAC+TGG - chr8_4:49081996-49082015 None:intergenic 45.0%
!!! GCCTGAAGCTTGTTTTGCAA+AGG + chr8_4:49082205-49082224 Msa1330050:intron 45.0%
GTTGGAGTTGCTAATAACGC+TGG + chr8_4:49082530-49082549 Msa1330050:CDS 45.0%
CATAGCTTAGTATCACCCAG+AGG + chr8_4:49082575-49082594 Msa1330050:CDS 45.0%
TATCACCCAGAGGATTGTCA+CGG + chr8_4:49082585-49082604 Msa1330050:CDS 45.0%
GAATAAGCTACCGCACCATA+TGG + chr8_4:49082616-49082635 Msa1330050:CDS 45.0%
TCATGCCCTTCGAAACCATA+TGG - chr8_4:49082634-49082653 None:intergenic 45.0%
! TATGGTTTCGAAGGGCATGA+AGG + chr8_4:49082634-49082653 Msa1330050:CDS 45.0%
! TGAAAAGTATGGTCGCAACG+AGG - chr8_4:49082880-49082899 None:intergenic 45.0%
ATTTCTCCATGAAGTGGGAG+GGG + chr8_4:49083047-49083066 Msa1330050:three_prime_UTR 45.0%
CACCAGCTATAGCTCCTGTA+AGG - chr8_4:49081188-49081207 None:intergenic 50.0%
! AGCTGGTGCTTTTGCAGCTA+AGG + chr8_4:49081200-49081219 Msa1330050:CDS 50.0%
GCAGCTAAGGCTACCAAAAG+TGG + chr8_4:49081213-49081232 Msa1330050:CDS 50.0%
! TCCCCGGAGAAAACCACTTT+TGG - chr8_4:49081229-49081248 None:intergenic 50.0%
!! GTTTCATTGGGTGCCATTGC+CGG + chr8_4:49081249-49081268 Msa1330050:CDS 50.0%
!! TTTCATTGGGTGCCATTGCC+GGG + chr8_4:49081250-49081269 Msa1330050:CDS 50.0%
! TATACTCTCGGTGGAGGTGT+TGG + chr8_4:49081275-49081294 Msa1330050:CDS 50.0%
TGGAAGCTTCCCGTGCTTAT+TGG + chr8_4:49081295-49081314 Msa1330050:CDS 50.0%
TCTCCTCCCTCCAGTTTGAT+TGG + chr8_4:49081983-49082002 Msa1330050:intron 50.0%
AAGCCAATCAAACTGGAGGG+AGG - chr8_4:49081989-49082008 None:intergenic 50.0%
CCACAAGCCAATCAAACTGG+AGG - chr8_4:49081993-49082012 None:intergenic 50.0%
!! CCTCCAGTTTGATTGGCTTG+TGG + chr8_4:49081990-49082009 Msa1330050:intron 50.0%
ATCACCCAGAGGATTGTCAC+GGG + chr8_4:49082586-49082605 Msa1330050:CDS 50.0%
GAATCCCGTGACAATCCTCT+GGG - chr8_4:49082593-49082612 None:intergenic 50.0%
TGAATCCCGTGACAATCCTC+TGG - chr8_4:49082594-49082613 None:intergenic 50.0%
ACCGCACCATATGGTTTCGA+AGG + chr8_4:49082625-49082644 Msa1330050:CDS 50.0%
CCCTTCGAAACCATATGGTG+CGG - chr8_4:49082629-49082648 None:intergenic 50.0%
CCGCACCATATGGTTTCGAA+GGG + chr8_4:49082626-49082645 Msa1330050:CDS 50.0%
TTTCTCCATGAAGTGGGAGG+GGG + chr8_4:49083048-49083067 Msa1330050:three_prime_UTR 50.0%
AAATTCCCCCTCCCACTTCA+TGG - chr8_4:49083056-49083075 None:intergenic 50.0%
AGCTGCTGTTGCTGCACCTA+TGG + chr8_4:49080396-49080415 Msa1330050:five_prime_UTR 55.0%
ATTGTAGCTGGGGCCCTTAC+AGG + chr8_4:49081171-49081190 Msa1330050:intron 55.0%
GCCCTTACAGGAGCTATAGC+TGG + chr8_4:49081183-49081202 Msa1330050:CDS 55.0%
TGGCACCCAATGAAACTCCC+CGG - chr8_4:49081245-49081264 None:intergenic 55.0%
!! TTCATTGGGTGCCATTGCCG+GGG + chr8_4:49081251-49081270 Msa1330050:CDS 55.0%
CATTGCCGGGGCTATACTCT+CGG + chr8_4:49081263-49081282 Msa1330050:CDS 55.0%
! TTCGAAGGGCATGAAGGCAG+AGG + chr8_4:49082640-49082659 Msa1330050:CDS 55.0%
! CGAGAGTATAGCCCCGGCAA+TGG - chr8_4:49081265-49081284 None:intergenic 60.0%
! CTCCACCGAGAGTATAGCCC+CGG - chr8_4:49081271-49081290 None:intergenic 60.0%
TTCACGCGGCGCGTCTTCTA+TGG + chr8_4:49081335-49081354 Msa1330050:CDS 60.0%
TGCCGGGGCTATACTCTCGG+TGG + chr8_4:49081266-49081285 Msa1330050:CDS 65.0%
CGGGGCTATACTCTCGGTGG+AGG + chr8_4:49081269-49081288 Msa1330050:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 49080243 49083225 49080243 ID=Msa1330050;Name=Msa1330050
chr8_4 mRNA 49080243 49083225 49080243 ID=Msa1330050-mRNA-1;Parent=Msa1330050;Name=Msa1330050-mRNA-1;_AED=0.32;_eAED=0.32;_QI=215|1|1|1|1|1|5|259|219
chr8_4 exon 49080243 49080563 49080243 ID=Msa1330050-mRNA-1:exon:14169;Parent=Msa1330050-mRNA-1
chr8_4 exon 49081178 49081356 49081178 ID=Msa1330050-mRNA-1:exon:14170;Parent=Msa1330050-mRNA-1
chr8_4 exon 49081569 49081652 49081569 ID=Msa1330050-mRNA-1:exon:14171;Parent=Msa1330050-mRNA-1
chr8_4 exon 49082521 49082688 49082521 ID=Msa1330050-mRNA-1:exon:14172;Parent=Msa1330050-mRNA-1
chr8_4 exon 49082844 49083225 49082844 ID=Msa1330050-mRNA-1:exon:14173;Parent=Msa1330050-mRNA-1
chr8_4 five_prime_UTR 49080243 49080457 49080243 ID=Msa1330050-mRNA-1:five_prime_utr;Parent=Msa1330050-mRNA-1
chr8_4 CDS 49080458 49080563 49080458 ID=Msa1330050-mRNA-1:cds;Parent=Msa1330050-mRNA-1
chr8_4 CDS 49081178 49081356 49081178 ID=Msa1330050-mRNA-1:cds;Parent=Msa1330050-mRNA-1
chr8_4 CDS 49081569 49081652 49081569 ID=Msa1330050-mRNA-1:cds;Parent=Msa1330050-mRNA-1
chr8_4 CDS 49082521 49082688 49082521 ID=Msa1330050-mRNA-1:cds;Parent=Msa1330050-mRNA-1
chr8_4 CDS 49082844 49082966 49082844 ID=Msa1330050-mRNA-1:cds;Parent=Msa1330050-mRNA-1
chr8_4 three_prime_UTR 49082967 49083225 49082967 ID=Msa1330050-mRNA-1:three_prime_utr;Parent=Msa1330050-mRNA-1
Gene Sequence

>Msa1330050

ATGGAAACTGGTGGACGAAACGAAATTCCCTTCTTGGGTTTTCGTTTTGTCTCCAAATTTCTATTATGCGCCTTTTCTGGAACTCTCACTGCATGTTTTGCCTTTGCTGGGGCCCTTACAGGAGCTATAGCTGGTGCTTTTGCAGCTAAGGCTACCAAAAGTGGTTTTCTCCGGGGAGTTTCATTGGGTGCCATTGCCGGGGCTATACTCTCGGTGGAGGTGTTGGAAGCTTCCCGTGCTTATTGGTGTATGGAACAAACTGGTTCACGCGGCGCGTCTTCTATGGCAGATTTCATAGAGGAACTTGTTCGTGGAAGGCTTGTAGAAGAATCACTTACACCTGCTATTTTAACTGCTTACAATTTGCAATTTGAGCAGGTTGGAGTTGCTAATAACGCTGGCTATGATGAGATTCATGATGTTCATAGCTTAGTATCACCCAGAGGATTGTCACGGGATTCATTGAATAAGCTACCGCACCATATGGTTTCGAAGGGCATGAAGGCAGAGGATACTTTCTGTGCAATATGTTTGCAGGACATTGAAGTTGGAGAAGTTGCAAGAAGCTTACCTCGTTGCGACCATACTTTTCACTTGATATGCGTGGACAAATGGCTTGTCAAGAATGATTCGTGTCCTATTTGCAGACAGAATGTGTAG
Protein sequence

>Msa1330050

METGGRNEIPFLGFRFVSKFLLCAFSGTLTACFAFAGALTGAIAGAFAAKATKSGFLRGVSLGAIAGAILSVEVLEASRAYWCMEQTGSRGASSMADFIEELVRGRLVEESLTPAILTAYNLQFEQVGVANNAGYDEIHDVHSLVSPRGLSRDSLNKLPHHMVSKGMKAEDTFCAICLQDIEVGEVARSLPRCDHTFHLICVDKWLVKNDSCPICRQNV*