AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1212250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212250 MtrunA17_Chr4g0060981 95.890 219 9 0 1 219 1 219 9.62e-149 412
Msa1212250 MtrunA17_Chr5g0406031 40.404 198 111 2 25 219 41 234 9.26e-52 167
Msa1212250 MtrunA17_Chr8g0371291 38.095 210 123 3 8 217 21 223 2.03e-46 153
Msa1212250 MtrunA17_Chr5g0444161 35.437 206 114 6 17 219 41 230 5.37e-27 103
Msa1212250 MtrunA17_Chr5g0444141 35.533 197 112 6 27 219 80 265 8.38e-26 101
Msa1212250 MtrunA17_Chr6g0451051 46.575 73 38 1 148 219 39 111 5.26e-16 72.4
Msa1212250 MtrunA17_Chr3g0128771 33.333 93 59 1 130 219 72 164 2.64e-15 71.2
Msa1212250 MtrunA17_Chr5g0437361 45.833 72 36 1 148 216 74 145 4.63e-15 70.1
Msa1212250 MtrunA17_Chr8g0383941 36.735 98 52 2 122 217 88 177 1.14e-14 70.1
Msa1212250 MtrunA17_Chr8g0383881 43.056 72 39 1 148 217 102 173 3.44e-14 68.9
Msa1212250 MtrunA17_Chr8g0383861 43.056 72 39 1 148 217 112 183 3.83e-14 68.9
Msa1212250 MtrunA17_Chr8g0388231 39.726 73 41 1 149 218 309 381 5.63e-14 70.1
Msa1212250 MtrunA17_Chr7g0246761 55.319 47 21 0 170 216 86 132 5.69e-14 67.8
Msa1212250 MtrunA17_Chr6g0451031 41.667 72 42 0 148 219 43 114 8.33e-14 65.9
Msa1212250 MtrunA17_Chr3g0118071 32.456 114 66 3 106 216 41 146 8.53e-14 67.8
Msa1212250 MtrunA17_Chr3g0120011 38.961 77 45 1 142 216 85 161 1.02e-13 67.4
Msa1212250 MtrunA17_Chr5g0430781 40.000 80 44 2 139 216 75 152 1.40e-13 67.4
Msa1212250 MtrunA17_Chr3g0143301 46.667 75 38 2 145 219 44 116 1.64e-13 65.1
Msa1212250 MtrunA17_Chr7g0274721 36.620 71 45 0 146 216 65 135 1.69e-13 66.2
Msa1212250 MtrunA17_Chr8g0383871 41.667 72 40 1 148 217 111 182 2.35e-13 66.6
Msa1212250 MtrunA17_Chr8g0383921 41.667 72 40 1 148 217 110 181 2.45e-13 66.6
Msa1212250 MtrunA17_Chr7g0244851 41.892 74 38 2 149 219 81 152 4.30e-13 65.1
Msa1212250 MtrunA17_Chr8g0370171 41.333 75 41 1 145 216 66 140 4.98e-13 65.5
Msa1212250 MtrunA17_Chr4g0030831 40.541 74 41 1 149 219 84 157 5.70e-13 64.7
Msa1212250 MtrunA17_Chr6g0486651 43.836 73 38 2 149 219 93 164 5.98e-13 67.0
Msa1212250 MtrunA17_Chr2g0327791 44.118 68 35 1 155 219 113 180 6.17e-13 65.5
Msa1212250 MtrunA17_Chr5g0407431 42.466 73 39 1 148 217 76 148 6.19e-13 65.5
Msa1212250 MtrunA17_Chr8g0383931 50.000 52 26 0 166 217 133 184 7.37e-13 65.5
Msa1212250 MtrunA17_Chr8g0383901 50.000 52 26 0 166 217 123 174 7.41e-13 65.1
Msa1212250 MtrunA17_Chr6g0451041 55.319 47 21 0 173 219 17 63 7.65e-13 62.4
Msa1212250 MtrunA17_Chr7g0248831 38.356 73 42 1 149 218 80 152 9.64e-13 64.3
Msa1212250 MtrunA17_Chr8g0348151 40.000 70 41 1 149 218 90 158 1.15e-12 64.3
Msa1212250 MtrunA17_Chr3g0086801 41.333 75 43 1 145 219 76 149 1.20e-12 63.5
Msa1212250 MtrunA17_Chr2g0295571 39.726 73 43 1 148 219 92 164 1.49e-12 65.5
Msa1212250 MtrunA17_Chr1g0157091 45.070 71 37 2 149 219 48 116 1.57e-12 62.4
Msa1212250 MtrunA17_Chr7g0248841 51.064 47 23 0 171 217 101 147 1.58e-12 63.2
Msa1212250 MtrunA17_Chr5g0426871 38.667 75 42 3 149 219 108 182 2.10e-12 65.5
Msa1212250 MtrunA17_Chr3g0113811 42.105 76 39 2 146 216 73 148 2.33e-12 63.2
Msa1212250 MtrunA17_Chr4g0067591 37.662 77 47 1 143 219 74 149 2.85e-12 64.3
Msa1212250 MtrunA17_Chr3g0118061 31.250 112 67 4 115 216 35 146 3.17e-12 63.5
Msa1212250 MtrunA17_Chr3g0129671 40.845 71 40 1 149 217 109 179 3.38e-12 63.5
Msa1212250 MtrunA17_Chr1g0184631 38.158 76 43 2 146 217 65 140 3.61e-12 62.8
Msa1212250 MtrunA17_Chr7g0274711 38.158 76 43 2 146 217 64 139 3.81e-12 62.4
Msa1212250 MtrunA17_Chr3g0105441 36.364 77 44 1 148 219 101 177 4.03e-12 63.9
Msa1212250 MtrunA17_Chr2g0307411 40.278 72 41 1 148 219 76 145 5.01e-12 62.4
Msa1212250 MtrunA17_Chr1g0167811 39.241 79 38 3 148 219 93 168 5.34e-12 63.9
Msa1212250 MtrunA17_Chr1g0191601 42.373 59 30 1 161 219 118 172 5.67e-12 63.9
Msa1212250 MtrunA17_Chr7g0274741 38.356 73 41 2 148 216 69 141 6.49e-12 62.0
Msa1212250 MtrunA17_Chr5g0441191 35.714 70 44 1 147 216 173 241 7.33e-12 63.5
Msa1212250 MtrunA17_Chr7g0274731 35.211 71 46 0 146 216 86 156 7.53e-12 62.0
Msa1212250 MtrunA17_Chr2g0325141 50.000 46 23 0 174 219 91 136 7.76e-12 60.8
Msa1212250 MtrunA17_Chr7g0273051 47.826 46 24 0 174 219 120 165 9.71e-12 63.5
Msa1212250 MtrunA17_Chr3g0132491 39.474 76 42 2 148 219 106 181 1.11e-11 63.2
Msa1212250 MtrunA17_Chr4g0013561 35.294 68 44 0 149 216 85 152 1.16e-11 62.0
Msa1212250 MtrunA17_Chr5g0409211 41.791 67 33 1 156 216 70 136 1.17e-11 61.2
Msa1212250 MtrunA17_Chr7g0276591 38.710 62 38 0 155 216 82 143 1.18e-11 62.8
Msa1212250 MtrunA17_Chr3g0144241 38.571 70 43 0 148 217 78 147 1.20e-11 61.6
Msa1212250 MtrunA17_Chr1g0198201 38.028 71 42 1 148 216 60 130 1.25e-11 62.0
Msa1212250 MtrunA17_Chr8g0345361 43.056 72 39 2 149 219 92 162 1.30e-11 62.4
Msa1212250 MtrunA17_Chr4g0073131 42.424 66 37 1 155 219 82 147 1.46e-11 60.8
Msa1212250 MtrunA17_Chr1g0184171 37.333 75 43 2 148 219 102 175 1.48e-11 62.8
Msa1212250 MtrunA17_Chr8g0366561 50.000 48 24 0 172 219 140 187 1.51e-11 62.8
Msa1212250 MtrunA17_Chr1g0171791 36.986 73 45 1 147 219 132 203 1.65e-11 62.0
Msa1212250 MtrunA17_Chr5g0409201 40.000 70 36 1 155 218 68 137 2.00e-11 60.1
Msa1212250 MtrunA17_Chr3g0117821 51.163 43 21 0 174 216 85 127 2.04e-11 60.1
Msa1212250 MtrunA17_Chr8g0388201 29.167 96 65 1 125 217 295 390 2.35e-11 62.4
Msa1212250 MtrunA17_Chr1g0169651 39.437 71 40 2 149 219 660 727 2.75e-11 62.4
Msa1212250 MtrunA17_Chr8g0369571 48.889 45 23 0 174 218 90 134 2.76e-11 60.1
Msa1212250 MtrunA17_Chr3g0126611 32.184 87 55 2 133 216 288 373 2.88e-11 62.0
Msa1212250 MtrunA17_Chr4g0073141 32.632 95 53 2 136 219 95 189 3.25e-11 62.0
Msa1212250 MtrunA17_Chr4g0027761 39.130 69 40 1 148 216 99 165 3.34e-11 60.5
Msa1212250 MtrunA17_Chr4g0052671 50.000 44 22 0 174 217 106 149 3.39e-11 59.3
Msa1212250 MtrunA17_Chr6g0470751 36.842 76 44 2 148 219 96 171 3.92e-11 61.2
Msa1212250 MtrunA17_Chr2g0325161 50.000 46 23 0 174 219 123 168 3.97e-11 60.8
Msa1212250 MtrunA17_Chr3g0133001 52.174 46 22 0 174 219 156 201 4.12e-11 61.6
Msa1212250 MtrunA17_Chr8g0350741 51.111 45 22 0 172 216 87 131 4.21e-11 59.7
Msa1212250 MtrunA17_Chr2g0279431 48.936 47 24 0 170 216 157 203 4.41e-11 61.6
Msa1212250 MtrunA17_Chr2g0300331 48.077 52 27 0 168 219 163 214 4.55e-11 60.5
Msa1212250 MtrunA17_Chr5g0427431 52.174 46 22 0 174 219 160 205 4.88e-11 61.6
Msa1212250 MtrunA17_Chr3g0126931 28.571 147 86 4 89 216 23 169 4.89e-11 61.2
Msa1212250 MtrunA17_Chr2g0297011 34.722 72 46 1 149 219 76 147 6.93e-11 58.9
Msa1212250 MtrunA17_Chr5g0396601 38.889 72 41 2 149 217 322 393 9.58e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212250 AT1G74410.1 56.373 204 84 2 17 219 22 221 1.26e-72 220
Msa1212250 AT5G66070.5 41.063 207 116 4 14 219 20 221 3.47e-49 160
Msa1212250 AT5G66070.1 41.063 207 116 4 14 219 20 221 3.47e-49 160
Msa1212250 AT5G66070.2 37.719 228 118 4 14 219 20 245 8.94e-47 155
Msa1212250 AT2G17730.1 38.462 208 120 4 16 219 38 241 1.33e-45 152
Msa1212250 AT4G35840.1 36.667 210 120 3 19 219 31 236 7.86e-44 147
Msa1212250 AT2G17730.2 38.191 199 115 4 16 210 38 232 2.13e-40 139
Msa1212250 AT4G35840.2 31.897 232 116 3 25 219 42 268 4.48e-37 130
Msa1212250 AT3G20395.1 29.825 228 134 5 5 219 2 216 1.26e-29 110
Msa1212250 AT5G66070.4 36.527 167 100 3 14 179 20 181 1.11e-26 101
Msa1212250 AT5G66070.3 33.511 188 101 4 14 179 20 205 2.28e-24 96.3
Msa1212250 AT3G10910.1 40.816 98 49 3 125 219 68 159 1.59e-16 74.7
Msa1212250 AT5G05280.1 41.667 72 42 0 148 219 87 158 1.66e-15 72.0
Msa1212250 AT2G27940.1 42.254 71 41 0 148 218 114 184 3.31e-15 72.4
Msa1212250 AT2G17450.1 44.872 78 35 2 148 217 68 145 1.97e-14 69.3
Msa1212250 AT5G01880.1 44.444 72 39 1 149 219 79 150 2.54e-14 68.6
Msa1212250 AT3G16720.1 38.961 77 47 0 143 219 88 164 6.62e-14 69.7
Msa1212250 AT4G10160.1 39.706 68 41 0 149 216 72 139 1.68e-13 67.4
Msa1212250 AT5G53110.1 37.363 91 51 2 131 218 287 374 2.02e-13 68.9
Msa1212250 AT5G42200.1 42.466 73 41 1 145 216 74 146 2.40e-13 65.9
Msa1212250 AT4G10150.1 38.235 68 42 0 149 216 86 153 4.62e-13 66.6
Msa1212250 AT4G17905.2 42.857 63 34 1 157 219 166 226 8.97e-13 66.6
Msa1212250 AT4G17905.1 42.857 63 34 1 157 219 140 200 9.01e-13 66.6
Msa1212250 AT4G09120.1 37.374 99 51 4 125 219 77 168 9.98e-13 66.6
Msa1212250 AT2G46160.1 39.241 79 39 1 149 218 104 182 1.13e-12 65.1
Msa1212250 AT2G46160.2 39.241 79 39 1 149 218 104 182 1.13e-12 65.1
Msa1212250 AT5G57750.1 37.879 66 39 1 155 218 101 166 1.16e-12 65.1
Msa1212250 AT1G20823.1 41.667 72 40 1 148 217 83 154 1.23e-12 64.7
Msa1212250 AT1G76410.1 35.165 91 57 1 129 217 57 147 1.63e-12 63.9
Msa1212250 AT1G35330.1 42.466 73 38 2 148 216 99 171 2.88e-12 65.1
Msa1212250 AT2G35910.1 40.580 69 40 1 149 216 121 189 3.48e-12 63.9
Msa1212250 AT2G42360.1 41.892 74 41 1 148 219 80 153 4.00e-12 63.9
Msa1212250 AT3G14320.1 52.174 46 22 0 174 219 88 133 4.90e-12 63.2
Msa1212250 AT4G40070.1 47.059 51 27 0 169 219 119 169 5.11e-12 64.3
Msa1212250 AT3G18930.2 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1212250 AT3G18930.3 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1212250 AT3G18930.1 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1212250 AT4G09130.1 38.710 93 50 4 131 219 76 165 5.84e-12 64.3
Msa1212250 AT3G18773.1 38.889 72 42 1 148 217 102 173 7.23e-12 62.8
Msa1212250 AT2G20030.1 39.726 73 41 2 149 219 98 169 7.47e-12 64.3
Msa1212250 AT5G10380.1 41.667 72 41 1 149 219 109 180 8.35e-12 63.5
Msa1212250 AT1G53820.1 50.000 46 23 0 174 219 120 165 9.22e-12 63.5
Msa1212250 AT1G49220.1 40.541 74 42 1 146 217 105 178 9.86e-12 63.2
Msa1212250 AT1G72310.1 37.333 75 47 0 145 219 98 172 1.11e-11 63.5
Msa1212250 AT2G42350.1 42.029 69 40 0 148 216 74 142 1.39e-11 62.0
Msa1212250 AT3G05200.1 52.174 46 22 0 174 219 128 173 1.48e-11 63.5
Msa1212250 AT4G30370.1 40.541 74 39 2 149 219 89 160 1.67e-11 61.2
Msa1212250 AT4G09100.1 34.615 78 47 1 146 219 53 130 1.82e-11 60.1
Msa1212250 AT4G28890.1 39.726 73 41 2 149 219 97 168 2.19e-11 62.8
Msa1212250 AT2G18670.1 39.726 73 42 1 149 219 83 155 2.36e-11 60.8
Msa1212250 AT2G40830.1 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212250 AT2G40830.3 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212250 AT2G40830.4 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212250 AT2G40830.2 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212250 AT1G33480.1 35.294 68 44 0 149 216 75 142 2.86e-11 61.6
Msa1212250 AT1G35625.1 38.889 72 40 3 150 218 207 277 3.32e-11 62.0
Msa1212250 AT1G28040.1 30.973 113 66 3 106 218 197 297 3.99e-11 61.6
Msa1212250 AT2G18650.1 35.616 73 45 1 149 219 99 171 4.00e-11 62.0
Msa1212250 AT3G48030.1 36.986 73 44 2 149 219 180 252 4.77e-11 61.6
Msa1212250 AT2G44578.1 42.308 52 30 0 168 219 64 115 4.77e-11 59.3
Msa1212250 AT4G30400.1 37.143 70 42 1 149 216 107 176 5.44e-11 62.0
Msa1212250 AT3G48030.2 36.986 73 44 2 149 219 102 174 5.56e-11 61.2
Msa1212250 AT1G53010.1 30.233 129 68 4 112 219 48 175 6.89e-11 59.3
Msa1212250 AT1G49200.1 38.961 77 39 2 146 217 106 179 7.58e-11 60.1
Msa1212250 AT5G17600.1 48.837 43 22 0 174 216 142 184 7.78e-11 61.2
Msa1212250 AT1G35630.1 41.667 72 38 3 150 218 207 277 9.07e-11 60.8
Msa1212250 AT2G25410.1 46.000 50 27 0 169 218 322 371 9.45e-11 60.8
Msa1212250 AT5G07040.1 35.616 73 44 1 149 218 66 138 9.91e-11 58.5

Find 78 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACCAAAATAGCATTAT+TGG 0.170704 8_1:+72096391 None:intergenic
GTTTCATTGGGTGCCATTGC+CGG 0.215022 8_1:-72098265 Msa1212250:CDS
CTAGTCCAATAATGCTATTT+TGG 0.245191 8_1:-72096396 Msa1212250:three_prime_UTR
ATTGTAGCTGGGGCCCTTAC+AGG 0.247414 8_1:-72098343 Msa1212250:intron
CAGCAGCTTATGATTGAAAA+TGG 0.286404 8_1:+72099135 None:intergenic
ACGAAACGAAATTCCCTTCT+TGG 0.308335 8_1:-72099044 Msa1212250:CDS
TGGAAGCTTCCCGTGCTTAT+TGG 0.315076 8_1:-72098219 Msa1212250:CDS
AAAGGCGCATAATAGAAATT+TGG 0.316639 8_1:+72099006 None:intergenic
TTTCATTGGGTGCCATTGCC+GGG 0.321224 8_1:-72098264 Msa1212250:CDS
TTCACGCGGCGCGTCTTCTA+TGG 0.328316 8_1:-72098179 Msa1212250:intron
CTCTGATTGGCTATCATTCA+TGG 0.356125 8_1:-72099077 Msa1212250:exon
TTCCATTGATTGATTGTTGA+CGG 0.369847 8_1:-72099213 Msa1212250:five_prime_UTR
CTGTGATATAATGATCATAA+AGG 0.370931 8_1:+72099268 None:intergenic
CGAAACGAAATTCCCTTCTT+GGG 0.382549 8_1:-72099043 Msa1212250:CDS
TTTCTGTGCAATATGTTTGC+AGG 0.383281 8_1:-72096845 Msa1212250:intron
TCCCCGGAGAAAACCACTTT+TGG 0.384936 8_1:+72098288 None:intergenic
TACACATTCTGTCTGCAAAT+AGG 0.395070 8_1:+72096569 None:intergenic
AATTTACAGGACATTGAAGT+TGG 0.400528 8_1:-72096677 Msa1212250:intron
TTTCTCCGGGGAGTTTCATT+GGG 0.418806 8_1:-72098277 Msa1212250:CDS
AGCTGCTGTTGCTGCACCTA+TGG 0.420837 8_1:-72099120 Msa1212250:five_prime_UTR
ACTTGATATGCGTGGACAAA+TGG 0.421325 8_1:-72096613 Msa1212250:CDS
AAAACATGATAACTGAGTAT+TGG 0.436148 8_1:+72096365 None:intergenic
CACCAGCTATAGCTCCTGTA+AGG 0.439321 8_1:+72098329 None:intergenic
TGAATCCCGTGACAATCCTC+TGG 0.451565 8_1:+72096921 None:intergenic
GAATAAGCTACCGCACCATA+TGG 0.453688 8_1:-72096896 Msa1212250:CDS
TGGCTATCATTCATGGAAAC+TGG 0.457921 8_1:-72099070 Msa1212250:exon
TTCCCGTGCTTATTGGTGTA+TGG 0.470182 8_1:-72098212 Msa1212250:CDS
ACGTTTCCACACTCTCTGAT+TGG 0.472939 8_1:-72099090 Msa1212250:five_prime_UTR
CGCATATCAAGTGAAAAGTA+TGG 0.479146 8_1:+72096624 None:intergenic
CATTGCCGGGGCTATACTCT+CGG 0.488812 8_1:-72098251 Msa1212250:CDS
TCATGCCCTTCGAAACCATA+TGG 0.493143 8_1:+72096881 None:intergenic
GCAGCTAAGGCTACCAAAAG+TGG 0.501253 8_1:-72098301 Msa1212250:CDS
GCCCTTACAGGAGCTATAGC+TGG 0.509484 8_1:-72098331 Msa1212250:CDS
ATTTCTCCATGAAGTGGGAG+GGG 0.523573 8_1:-72096462 Msa1212250:three_prime_UTR
TTTCTCCATGAAGTGGGAGG+GGG 0.525103 8_1:-72096461 Msa1212250:three_prime_UTR
TATACTCTCGGTGGAGGTGT+TGG 0.526459 8_1:-72098239 Msa1212250:CDS
TTGTAAGCAGTTAAAATAGC+AGG 0.527550 8_1:+72097889 None:intergenic
TAGAGGAACTTGTTCGTGGA+AGG 0.529453 8_1:-72097933 Msa1212250:CDS
TGCAGTGAGAGTTCCAGAAA+AGG 0.530821 8_1:+72098988 None:intergenic
AGCCGTCAACAATCAATCAA+TGG 0.532462 8_1:+72099211 None:intergenic
TTCATAGAGGAACTTGTTCG+TGG 0.535580 8_1:-72097937 Msa1212250:CDS
CTCCACCGAGAGTATAGCCC+CGG 0.540703 8_1:+72098246 None:intergenic
TCAATGGAAAATGAAAAGTA+AGG 0.543772 8_1:+72099227 None:intergenic
GACAAAACGAAAACCCAAGA+AGG 0.544046 8_1:+72099030 None:intergenic
TTATTTCTCCATGAAGTGGG+AGG 0.545078 8_1:-72096464 Msa1212250:three_prime_UTR
TATTGGTGTATGGAACAAAC+TGG 0.545201 8_1:-72098202 Msa1212250:CDS
ACCGCACCATATGGTTTCGA+AGG 0.553055 8_1:-72096887 Msa1212250:CDS
TATTTCTCCATGAAGTGGGA+GGG 0.558759 8_1:-72096463 Msa1212250:three_prime_UTR
TTCATTGGGTGCCATTGCCG+GGG 0.560934 8_1:-72098263 Msa1212250:CDS
TGGAAACGTTTATGAGCCAT+AGG 0.562502 8_1:+72099104 None:intergenic
CGAGAGTATAGCCCCGGCAA+TGG 0.563534 8_1:+72098252 None:intergenic
TTCCATACACCAATAAGCAC+GGG 0.567244 8_1:+72098210 None:intergenic
TTCGAAGGGCATGAAGGCAG+AGG 0.573221 8_1:-72096872 Msa1212250:CDS
AGCTTATGATTGAAAATGGA+AGG 0.578222 8_1:+72099139 None:intergenic
AATCCAGGCAGATTTCATAG+AGG 0.579193 8_1:-72097950 Msa1212250:intron
GAAAATGAAAAGTAAGGTGT+TGG 0.587260 8_1:+72099233 None:intergenic
TGATAGCCAATCAGAGAGTG+TGG 0.589978 8_1:+72099084 None:intergenic
GTTCCATACACCAATAAGCA+CGG 0.597188 8_1:+72098209 None:intergenic
TATTGGACTAGATTACAGAA+TGG 0.600503 8_1:+72096408 None:intergenic
CTACTGCACTTAGATTTAGT+CGG 0.600879 8_1:+72096303 None:intergenic
CGGGGCTATACTCTCGGTGG+AGG 0.602147 8_1:-72098245 Msa1212250:CDS
TATGGTTTCGAAGGGCATGA+AGG 0.614703 8_1:-72096878 Msa1212250:CDS
AAGGACAAGGAAGATGAAGC+TGG 0.618453 8_1:+72099158 None:intergenic
ACAAAACGAAAACCCAAGAA+GGG 0.623335 8_1:+72099031 None:intergenic
TGGCACCCAATGAAACTCCC+CGG 0.623545 8_1:+72098272 None:intergenic
CTATCATTCATGGAAACTGG+TGG 0.623614 8_1:-72099067 Msa1212250:exon
GTTGGAGTTGCTAATAACGC+TGG 0.623875 8_1:-72096982 Msa1212250:CDS
GAATCCCGTGACAATCCTCT+GGG 0.625719 8_1:+72096922 None:intergenic
ATCACCCAGAGGATTGTCAC+GGG 0.633383 8_1:-72096926 Msa1212250:CDS
ACCAGCTATAGCTCCTGTAA+GGG 0.637027 8_1:+72098330 None:intergenic
CCGCACCATATGGTTTCGAA+GGG 0.637296 8_1:-72096886 Msa1212250:CDS
CCCTTCGAAACCATATGGTG+CGG 0.643401 8_1:+72096886 None:intergenic
TATCACCCAGAGGATTGTCA+CGG 0.646030 8_1:-72096927 Msa1212250:CDS
TGCCGGGGCTATACTCTCGG+TGG 0.658573 8_1:-72098248 Msa1212250:CDS
ATGGAACAAACTGGTTCACG+CGG 0.720989 8_1:-72098193 Msa1212250:CDS
CATAGCTTAGTATCACCCAG+AGG 0.736019 8_1:-72096937 Msa1212250:CDS
TGATTGAAAATGGAAGGACA+AGG 0.736730 8_1:+72099145 None:intergenic
TGAAAAGTATGGTCGCAACG+AGG 0.774791 8_1:+72096635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAAAACAAATTATAAA+TGG + chr8_1:72098392-72098411 None:intergenic 10.0%
!!! TTATTTTGTGTTAATATTTA+CGG - chr8_1:72099048-72099067 Msa1212250:CDS 10.0%
!!! TTTTTTTGTTTGATTTGAAT+TGG - chr8_1:72096750-72096769 Msa1212250:intron 15.0%
!!! TATGAAAGTTTAAGAATTTT+TGG - chr8_1:72096829-72096848 Msa1212250:intron 15.0%
!!! ATGAAAGTTTAAGAATTTTT+GGG - chr8_1:72096830-72096849 Msa1212250:intron 15.0%
!! TCAGAATTTAAATATGATAT+AGG + chr8_1:72098113-72098132 None:intergenic 15.0%
!!! TATTTTGTGTTAATATTTAC+GGG - chr8_1:72099049-72099068 Msa1212250:CDS 15.0%
!!! TGAAAGTTTAAGAATTTTTG+GGG - chr8_1:72096831-72096850 Msa1212250:intron 20.0%
!!! ATAATTTGTTTATCCAAACA+GGG + chr8_1:72096896-72096915 None:intergenic 20.0%
!!! AATAATTTGTTTATCCAAAC+AGG + chr8_1:72096897-72096916 None:intergenic 20.0%
!! TCAAATAAACTAATTCAAAC+AGG - chr8_1:72097154-72097173 Msa1212250:intron 20.0%
!!! AATTGGTTTAAAATATGCAT+CGG + chr8_1:72097603-72097622 None:intergenic 20.0%
!!! AAATTCTGAAACTAAAAAAG+AGG - chr8_1:72098124-72098143 Msa1212250:intron 20.0%
!! TACTATGTGTTAAATTTGTA+TGG + chr8_1:72098238-72098257 None:intergenic 20.0%
!! ATACAAATTTAACACATAGT+AGG - chr8_1:72098237-72098256 Msa1212250:CDS 20.0%
!! ATTGAGTTATGTATATACAT+TGG - chr8_1:72098303-72098322 Msa1212250:CDS 20.0%
!! CATTGAGAAATATAATTTAC+AGG - chr8_1:72098895-72098914 Msa1212250:intron 20.0%
! CTGTGATATAATGATCATAA+AGG + chr8_1:72096320-72096339 None:intergenic 25.0%
! TCAATGGAAAATGAAAAGTA+AGG + chr8_1:72096361-72096380 None:intergenic 25.0%
! TTGCACAAATCAATTTATTC+TGG - chr8_1:72096715-72096734 Msa1212250:intron 25.0%
!! TAAAAGAATTGCTATTGACT+AGG + chr8_1:72096870-72096889 None:intergenic 25.0%
!! TAGTCAATAGCAATTCTTTT+AGG - chr8_1:72096869-72096888 Msa1212250:CDS 25.0%
!! AGTCAATAGCAATTCTTTTA+GGG - chr8_1:72096870-72096889 Msa1212250:CDS 25.0%
! GTTTATCATATAAGCACTTA+CGG - chr8_1:72096938-72096957 Msa1212250:CDS 25.0%
! TTTATCATATAAGCACTTAC+GGG - chr8_1:72096939-72096958 Msa1212250:CDS 25.0%
! GTTTATTTGAGCTTATCTAT+TGG + chr8_1:72097144-72097163 None:intergenic 25.0%
! CTTGACATGCATATATATAT+AGG - chr8_1:72097496-72097515 Msa1212250:intron 25.0%
! GGATTTAAATGTTACAGAAT+TGG + chr8_1:72097620-72097639 None:intergenic 25.0%
! ATTCTGTAACATTTAAATCC+AGG - chr8_1:72097620-72097639 Msa1212250:intron 25.0%
!!! TTTTAAAAGGCAAAAATTGG+GGG + chr8_1:72097752-72097771 None:intergenic 25.0%
!!! CTTTTAAAAGGCAAAAATTG+GGG + chr8_1:72097753-72097772 None:intergenic 25.0%
!!! GCTTTTAAAAGGCAAAAATT+GGG + chr8_1:72097754-72097773 None:intergenic 25.0%
!!! TGCTTTTAAAAGGCAAAAAT+TGG + chr8_1:72097755-72097774 None:intergenic 25.0%
!!! TAGTTTTATGAGCTTCTTTT+GGG - chr8_1:72097980-72097999 Msa1212250:intron 25.0%
! ATCTTTAAATGACCTACATT+GGG + chr8_1:72098475-72098494 None:intergenic 25.0%
! AATCTTTAAATGACCTACAT+TGG + chr8_1:72098476-72098495 None:intergenic 25.0%
! TTTCAGGATTTGTGATTTAA+TGG - chr8_1:72098773-72098792 Msa1212250:intron 25.0%
! ATAGGGAAAATGAAAAATTG+TGG + chr8_1:72098832-72098851 None:intergenic 25.0%
! TATCATCCAAATCTCATATA+GGG + chr8_1:72098849-72098868 None:intergenic 25.0%
! ATATCATCCAAATCTCATAT+AGG + chr8_1:72098850-72098869 None:intergenic 25.0%
!!! TTTTTTATTTCTCCATGAAG+TGG - chr8_1:72099117-72099136 Msa1212250:five_prime_UTR 25.0%
!!! TTTTTATTTCTCCATGAAGT+GGG - chr8_1:72099118-72099137 Msa1212250:five_prime_UTR 25.0%
! TTCTACCAAAATAGCATTAT+TGG + chr8_1:72099197-72099216 None:intergenic 25.0%
! AAAACATGATAACTGAGTAT+TGG + chr8_1:72099223-72099242 None:intergenic 25.0%
GAAAATGAAAAGTAAGGTGT+TGG + chr8_1:72096355-72096374 None:intergenic 30.0%
! TTCCATTGATTGATTGTTGA+CGG - chr8_1:72096372-72096391 Msa1212250:three_prime_UTR 30.0%
! ATGAAGCTGGTTAGATTTTT+GGG + chr8_1:72096417-72096436 None:intergenic 30.0%
AGCTTATGATTGAAAATGGA+AGG + chr8_1:72096449-72096468 None:intergenic 30.0%
! AAAGGCGCATAATAGAAATT+TGG + chr8_1:72096582-72096601 None:intergenic 30.0%
AGGATATTAGAGATTACCAA+AGG + chr8_1:72096630-72096649 None:intergenic 30.0%
AAATCATCATAGATGGTACA+AGG + chr8_1:72096650-72096669 None:intergenic 30.0%
TTGTACCATCTATGATGATT+TGG - chr8_1:72096649-72096668 Msa1212250:CDS 30.0%
AATTACGAAGCTTAAACATG+AGG - chr8_1:72096682-72096701 Msa1212250:intron 30.0%
ATTACGAAGCTTAAACATGA+GGG - chr8_1:72096683-72096702 Msa1212250:intron 30.0%
ATTGGTTGTAGTGAAATTCA+TGG - chr8_1:72096768-72096787 Msa1212250:intron 30.0%
TTGTAGTGAAATTCATGGTT+TGG - chr8_1:72096773-72096792 Msa1212250:intron 30.0%
!!! TTTAAGAATTTTTGGGGTGT+TGG - chr8_1:72096837-72096856 Msa1212250:intron 30.0%
!! GTTATTTCTATAAGCTCTTC+AGG + chr8_1:72097042-72097061 None:intergenic 30.0%
AAACTTATGAAAACAGCTTC+CGG + chr8_1:72097087-72097106 None:intergenic 30.0%
!! TTGTGTTTTTCAATTGTAGC+TGG - chr8_1:72097230-72097249 Msa1212250:intron 30.0%
!! TGTGTTTTTCAATTGTAGCT+GGG - chr8_1:72097231-72097250 Msa1212250:intron 30.0%
! TTCACCTTGATTTTTGTATG+TGG - chr8_1:72097458-72097477 Msa1212250:intron 30.0%
TTGTCCACATACAAAAATCA+AGG + chr8_1:72097465-72097484 None:intergenic 30.0%
TAAGATGCATCTTAAAAACC+AGG + chr8_1:72097554-72097573 None:intergenic 30.0%
!!! TTAAGAGTGTGTTTTTTGAG+TGG - chr8_1:72097571-72097590 Msa1212250:intron 30.0%
! TTTAAAATATGCATCGGAGA+TGG + chr8_1:72097597-72097616 None:intergenic 30.0%
TTGTAAGCAGTTAAAATAGC+AGG + chr8_1:72097699-72097718 None:intergenic 30.0%
!! ACAAAGCTACTGCTTTTAAA+AGG + chr8_1:72097765-72097784 None:intergenic 30.0%
ATATAATAGTGTAGTTGCGA+TGG - chr8_1:72097806-72097825 Msa1212250:intron 30.0%
! ACGGAAAATTTGCATTTTTC+AGG + chr8_1:72097947-72097966 None:intergenic 30.0%
!!! CTAGTTTTATGAGCTTCTTT+TGG - chr8_1:72097979-72097998 Msa1212250:intron 30.0%
! GAGGAGAAAAAAACATTTTC+AGG + chr8_1:72098043-72098062 None:intergenic 30.0%
TGATTACATGAATCTCTTTC+TGG - chr8_1:72098425-72098444 Msa1212250:intron 30.0%
TCTTTAAATGACCTACATTG+GGG + chr8_1:72098474-72098493 None:intergenic 30.0%
!!! TCATTTGTTTTAGTTTGAGC+AGG - chr8_1:72098581-72098600 Msa1212250:intron 30.0%
! CATTTTCCCTATATGAGATT+TGG - chr8_1:72098840-72098859 Msa1212250:intron 30.0%
AATTTACAGGACATTGAAGT+TGG - chr8_1:72098908-72098927 Msa1212250:intron 30.0%
! TATTGGACTAGATTACAGAA+TGG + chr8_1:72099180-72099199 None:intergenic 30.0%
!! CTAGTCCAATAATGCTATTT+TGG - chr8_1:72099189-72099208 Msa1212250:five_prime_UTR 30.0%
! TGAAGCTGGTTAGATTTTTG+GGG + chr8_1:72096416-72096435 None:intergenic 35.0%
! GATGAAGCTGGTTAGATTTT+TGG + chr8_1:72096418-72096437 None:intergenic 35.0%
TGATTGAAAATGGAAGGACA+AGG + chr8_1:72096443-72096462 None:intergenic 35.0%
CAGCAGCTTATGATTGAAAA+TGG + chr8_1:72096453-72096472 None:intergenic 35.0%
ACAAAACGAAAACCCAAGAA+GGG + chr8_1:72096557-72096576 None:intergenic 35.0%
! TTTCTATTATGCGCCTTTTC+TGG - chr8_1:72096584-72096603 Msa1212250:CDS 35.0%
AACAGCCAAATCATCATAGA+TGG + chr8_1:72096657-72096676 None:intergenic 35.0%
!!! TTTTTGTTGGCTTCTTCTCA+TGG - chr8_1:72097206-72097225 Msa1212250:intron 35.0%
!! GTGTTTTTCAATTGTAGCTG+GGG - chr8_1:72097232-72097251 Msa1212250:intron 35.0%
TATTGGTGTATGGAACAAAC+TGG - chr8_1:72097383-72097402 Msa1212250:intron 35.0%
TCTATGGTAAGTGATCGTTA+TGG - chr8_1:72097422-72097441 Msa1212250:intron 35.0%
TCATAAAACTAGAGAAGAGC+GGG + chr8_1:72097971-72097990 None:intergenic 35.0%
CTCATAAAACTAGAGAAGAG+CGG + chr8_1:72097972-72097991 None:intergenic 35.0%
AGGTGCAATGAAAGAAAATG+AGG + chr8_1:72098023-72098042 None:intergenic 35.0%
!!! TTGTTTTAGTTTGAGCAGGT+TGG - chr8_1:72098585-72098604 Msa1212250:intron 35.0%
TTTCTGTGCAATATGTTTGC+AGG - chr8_1:72098740-72098759 Msa1212250:intron 35.0%
! TGCAGGTGATTATTCTTTTC+AGG - chr8_1:72098757-72098776 Msa1212250:intron 35.0%
CGCATATCAAGTGAAAAGTA+TGG + chr8_1:72098964-72098983 None:intergenic 35.0%
! TACTTTTCACTTGATATGCG+TGG - chr8_1:72098964-72098983 Msa1212250:intron 35.0%
TACACATTCTGTCTGCAAAT+AGG + chr8_1:72099019-72099038 None:intergenic 35.0%
AGCCGTCAACAATCAATCAA+TGG + chr8_1:72096377-72096396 None:intergenic 40.0%
TGGAAACGTTTATGAGCCAT+AGG + chr8_1:72096484-72096503 None:intergenic 40.0%
! CTCTGATTGGCTATCATTCA+TGG - chr8_1:72096508-72096527 Msa1212250:three_prime_UTR 40.0%
TGGCTATCATTCATGGAAAC+TGG - chr8_1:72096515-72096534 Msa1212250:three_prime_UTR 40.0%
CTATCATTCATGGAAACTGG+TGG - chr8_1:72096518-72096537 Msa1212250:three_prime_UTR 40.0%
ACGAAACGAAATTCCCTTCT+TGG - chr8_1:72096541-72096560 Msa1212250:three_prime_UTR 40.0%
CGAAACGAAATTCCCTTCTT+GGG - chr8_1:72096542-72096561 Msa1212250:three_prime_UTR 40.0%
GACAAAACGAAAACCCAAGA+AGG + chr8_1:72096558-72096577 None:intergenic 40.0%
!!! AATTCTTTTAGGGCCCTGTT+TGG - chr8_1:72096880-72096899 Msa1212250:CDS 40.0%
GAAACAGCTAATGAACATGC+CGG - chr8_1:72097065-72097084 Msa1212250:intron 40.0%
TCAAAGCAACACTTAGGTCA+GGG + chr8_1:72097180-72097199 None:intergenic 40.0%
!!! GCTTTGAGGTGTGTTTTTGT+TGG - chr8_1:72097193-72097212 Msa1212250:intron 40.0%
! CTACCAAAAGTGGTTTTCTC+CGG - chr8_1:72097294-72097313 Msa1212250:intron 40.0%
! TACCAAAAGTGGTTTTCTCC+GGG - chr8_1:72097295-72097314 Msa1212250:intron 40.0%
TTCCATACACCAATAAGCAC+GGG + chr8_1:72097378-72097397 None:intergenic 40.0%
GTTCCATACACCAATAAGCA+CGG + chr8_1:72097379-72097398 None:intergenic 40.0%
GCAGCACAAATCACAATACA+AGG + chr8_1:72097532-72097551 None:intergenic 40.0%
AATCCAGGCAGATTTCATAG+AGG - chr8_1:72097635-72097654 Msa1212250:intron 40.0%
! TTCATAGAGGAACTTGTTCG+TGG - chr8_1:72097648-72097667 Msa1212250:intron 40.0%
TGACAGTATCAAGAGTCGTT+CGG - chr8_1:72097841-72097860 Msa1212250:intron 40.0%
!!! GTCTGAAGCTTGTTTTGCAA+AGG - chr8_1:72098278-72098297 Msa1212250:CDS 40.0%
GTTGCAAAAATCAACAAGGG+GGG + chr8_1:72098452-72098471 None:intergenic 40.0%
GGTTGCAAAAATCAACAAGG+GGG + chr8_1:72098453-72098472 None:intergenic 40.0%
GGGTTGCAAAAATCAACAAG+GGG + chr8_1:72098454-72098473 None:intergenic 40.0%
GGGGTTGCAAAAATCAACAA+GGG + chr8_1:72098455-72098474 None:intergenic 40.0%
TGGGGTTGCAAAAATCAACA+AGG + chr8_1:72098456-72098475 None:intergenic 40.0%
! GATTTTTGCAACCCCAATGT+AGG - chr8_1:72098460-72098479 Msa1212250:intron 40.0%
TGCTACAAACAAATCAACGC+AGG + chr8_1:72098537-72098556 None:intergenic 40.0%
ACTTGATATGCGTGGACAAA+TGG - chr8_1:72098972-72098991 Msa1212250:intron 40.0%
! TTATTTCTCCATGAAGTGGG+AGG - chr8_1:72099121-72099140 Msa1212250:five_prime_UTR 40.0%
TATTTCTCCATGAAGTGGGA+GGG - chr8_1:72099122-72099141 Msa1212250:five_prime_UTR 40.0%
AAGGACAAGGAAGATGAAGC+TGG + chr8_1:72096430-72096449 None:intergenic 45.0%
ACGTTTCCACACTCTCTGAT+TGG - chr8_1:72096495-72096514 Msa1212250:three_prime_UTR 45.0%
TGATAGCCAATCAGAGAGTG+TGG + chr8_1:72096504-72096523 None:intergenic 45.0%
TGCAGTGAGAGTTCCAGAAA+AGG + chr8_1:72096600-72096619 None:intergenic 45.0%
! CTCACTGCATGTTTTGCCTT+TGG - chr8_1:72096611-72096630 Msa1212250:CDS 45.0%
CTCAAAGCAACACTTAGGTC+AGG + chr8_1:72097181-72097200 None:intergenic 45.0%
!! CTGACCTAAGTGTTGCTTTG+AGG - chr8_1:72097179-72097198 Msa1212250:intron 45.0%
CACACCTCAAAGCAACACTT+AGG + chr8_1:72097186-72097205 None:intergenic 45.0%
ACCAGCTATAGCTCCTGTAA+GGG + chr8_1:72097258-72097277 None:intergenic 45.0%
! ACCAAAAGTGGTTTTCTCCG+GGG - chr8_1:72097296-72097315 Msa1212250:intron 45.0%
! TTTTCTCCGGGGAGTTTCAT+TGG - chr8_1:72097307-72097326 Msa1212250:intron 45.0%
TTTCTCCGGGGAGTTTCATT+GGG - chr8_1:72097308-72097327 Msa1212250:intron 45.0%
TTCCCGTGCTTATTGGTGTA+TGG - chr8_1:72097373-72097392 Msa1212250:intron 45.0%
! ATGGAACAAACTGGTTCACG+CGG - chr8_1:72097392-72097411 Msa1212250:intron 45.0%
! GTATTGTGATTTGTGCTGCC+TGG - chr8_1:72097533-72097552 Msa1212250:intron 45.0%
GTTCCTCTATGAAATCTGCC+TGG + chr8_1:72097641-72097660 None:intergenic 45.0%
! TAGAGGAACTTGTTCGTGGA+AGG - chr8_1:72097652-72097671 Msa1212250:intron 45.0%
TTGGGGGAGAGATATAGAGA+AGG + chr8_1:72097736-72097755 None:intergenic 45.0%
GTATCAAGAGTCGTTCGGTA+CGG - chr8_1:72097846-72097865 Msa1212250:intron 45.0%
AAACTAGAGAAGAGCGGGAA+CGG + chr8_1:72097966-72097985 None:intergenic 45.0%
CACAAGCCAATCAAACTGGA+GGG + chr8_1:72098065-72098084 None:intergenic 45.0%
TCACCACAAGCCAATCAAAC+TGG + chr8_1:72098069-72098088 None:intergenic 45.0%
GTTGGAGTTGCTAATAACGC+TGG - chr8_1:72098603-72098622 Msa1212250:intron 45.0%
CATAGCTTAGTATCACCCAG+AGG - chr8_1:72098648-72098667 Msa1212250:intron 45.0%
TATCACCCAGAGGATTGTCA+CGG - chr8_1:72098658-72098677 Msa1212250:intron 45.0%
GAATAAGCTACCGCACCATA+TGG - chr8_1:72098689-72098708 Msa1212250:intron 45.0%
TCATGCCCTTCGAAACCATA+TGG + chr8_1:72098707-72098726 None:intergenic 45.0%
! TATGGTTTCGAAGGGCATGA+AGG - chr8_1:72098707-72098726 Msa1212250:intron 45.0%
! TGAAAAGTATGGTCGCAACG+AGG + chr8_1:72098953-72098972 None:intergenic 45.0%
ATTTCTCCATGAAGTGGGAG+GGG - chr8_1:72099123-72099142 Msa1212250:five_prime_UTR 45.0%
CACCAGCTATAGCTCCTGTA+AGG + chr8_1:72097259-72097278 None:intergenic 50.0%
! AGCTGGTGCTTTTGCAGCTA+AGG - chr8_1:72097271-72097290 Msa1212250:intron 50.0%
GCAGCTAAGGCTACCAAAAG+TGG - chr8_1:72097284-72097303 Msa1212250:intron 50.0%
! TCCCCGGAGAAAACCACTTT+TGG + chr8_1:72097300-72097319 None:intergenic 50.0%
!! GTTTCATTGGGTGCCATTGC+CGG - chr8_1:72097320-72097339 Msa1212250:intron 50.0%
!! TTTCATTGGGTGCCATTGCC+GGG - chr8_1:72097321-72097340 Msa1212250:intron 50.0%
! TATACTCTCGGTGGAGGTGT+TGG - chr8_1:72097346-72097365 Msa1212250:intron 50.0%
TGGAAGCTTCCCGTGCTTAT+TGG - chr8_1:72097366-72097385 Msa1212250:intron 50.0%
! CGGTACGGCTAATTTGTGAC+AGG - chr8_1:72097861-72097880 Msa1212250:intron 50.0%
TCTCCTCCCTCCAGTTTGAT+TGG - chr8_1:72098056-72098075 Msa1212250:intron 50.0%
AAGCCAATCAAACTGGAGGG+AGG + chr8_1:72098062-72098081 None:intergenic 50.0%
CCACAAGCCAATCAAACTGG+AGG + chr8_1:72098066-72098085 None:intergenic 50.0%
!! CCTCCAGTTTGATTGGCTTG+TGG - chr8_1:72098063-72098082 Msa1212250:intron 50.0%
ATCACCCAGAGGATTGTCAC+GGG - chr8_1:72098659-72098678 Msa1212250:intron 50.0%
GAATCCCGTGACAATCCTCT+GGG + chr8_1:72098666-72098685 None:intergenic 50.0%
TGAATCCCGTGACAATCCTC+TGG + chr8_1:72098667-72098686 None:intergenic 50.0%
ACCGCACCATATGGTTTCGA+AGG - chr8_1:72098698-72098717 Msa1212250:intron 50.0%
CCCTTCGAAACCATATGGTG+CGG + chr8_1:72098702-72098721 None:intergenic 50.0%
CCGCACCATATGGTTTCGAA+GGG - chr8_1:72098699-72098718 Msa1212250:intron 50.0%
TTTCTCCATGAAGTGGGAGG+GGG - chr8_1:72099124-72099143 Msa1212250:five_prime_UTR 50.0%
AAATTCCCCCTCCCACTTCA+TGG + chr8_1:72099132-72099151 None:intergenic 50.0%
AGCTGCTGTTGCTGCACCTA+TGG - chr8_1:72096465-72096484 Msa1212250:three_prime_UTR 55.0%
ATTGTAGCTGGGGCCCTTAC+AGG - chr8_1:72097242-72097261 Msa1212250:intron 55.0%
GCCCTTACAGGAGCTATAGC+TGG - chr8_1:72097254-72097273 Msa1212250:intron 55.0%
TGGCACCCAATGAAACTCCC+CGG + chr8_1:72097316-72097335 None:intergenic 55.0%
!! TTCATTGGGTGCCATTGCCG+GGG - chr8_1:72097322-72097341 Msa1212250:intron 55.0%
CATTGCCGGGGCTATACTCT+CGG - chr8_1:72097334-72097353 Msa1212250:intron 55.0%
! TTCGAAGGGCATGAAGGCAG+AGG - chr8_1:72098713-72098732 Msa1212250:intron 55.0%
! CGAGAGTATAGCCCCGGCAA+TGG + chr8_1:72097336-72097355 None:intergenic 60.0%
! CTCCACCGAGAGTATAGCCC+CGG + chr8_1:72097342-72097361 None:intergenic 60.0%
TTCACGCGGCGCGTCTTCTA+TGG - chr8_1:72097406-72097425 Msa1212250:intron 60.0%
TGCCGGGGCTATACTCTCGG+TGG - chr8_1:72097337-72097356 Msa1212250:intron 65.0%
CGGGGCTATACTCTCGGTGG+AGG - chr8_1:72097340-72097359 Msa1212250:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 72096307 72099300 72096307 ID=Msa1212250;Name=Msa1212250
chr8_1 mRNA 72096307 72099300 72096307 ID=Msa1212250-mRNA-1;Parent=Msa1212250;Name=Msa1212250-mRNA-1;_AED=0.35;_eAED=0.35;_QI=220|1|1|1|1|1|5|261|219
chr8_1 exon 72096307 72096690 72096307 ID=Msa1212250-mRNA-1:exon:24327;Parent=Msa1212250-mRNA-1
chr8_1 exon 72096846 72097013 72096846 ID=Msa1212250-mRNA-1:exon:24326;Parent=Msa1212250-mRNA-1
chr8_1 exon 72097882 72097965 72097882 ID=Msa1212250-mRNA-1:exon:24325;Parent=Msa1212250-mRNA-1
chr8_1 exon 72098180 72098358 72098180 ID=Msa1212250-mRNA-1:exon:24324;Parent=Msa1212250-mRNA-1
chr8_1 exon 72098975 72099300 72098975 ID=Msa1212250-mRNA-1:exon:24323;Parent=Msa1212250-mRNA-1
chr8_1 five_prime_UTR 72099081 72099300 72099081 ID=Msa1212250-mRNA-1:five_prime_utr;Parent=Msa1212250-mRNA-1
chr8_1 CDS 72098975 72099080 72098975 ID=Msa1212250-mRNA-1:cds;Parent=Msa1212250-mRNA-1
chr8_1 CDS 72098180 72098358 72098180 ID=Msa1212250-mRNA-1:cds;Parent=Msa1212250-mRNA-1
chr8_1 CDS 72097882 72097965 72097882 ID=Msa1212250-mRNA-1:cds;Parent=Msa1212250-mRNA-1
chr8_1 CDS 72096846 72097013 72096846 ID=Msa1212250-mRNA-1:cds;Parent=Msa1212250-mRNA-1
chr8_1 CDS 72096568 72096690 72096568 ID=Msa1212250-mRNA-1:cds;Parent=Msa1212250-mRNA-1
chr8_1 three_prime_UTR 72096307 72096567 72096307 ID=Msa1212250-mRNA-1:three_prime_utr;Parent=Msa1212250-mRNA-1
Gene Sequence

>Msa1212250

ATGGAAACTGGTGGACGAAACGAAATTCCCTTCTTGGGTTTTCGTTTTGTCTCCAAATTTCTATTATGCGCCTTTTCTGGAACTCTCACTGCATGTTTTGCCTTTGCTGGGGCCCTTACAGGAGCTATAGCTGGTGCTTTTGCAGCTAAGGCTACCAAAAGTGGTTTTCTCCGGGGAGTTTCATTGGGTGCCATTGCCGGGGCTATACTCTCGGTGGAGGTGTTGGAAGCTTCCCGTGCTTATTGGTGTATGGAACAAACTGGTTCACGCGGCGCGTCTTCTATGGCAGATTTCATAGAGGAACTTGTTCGTGGAAGGCTTGTAGAAGAATCACTTACACCTGCTATTTTAACTGCTTACAATTTGCAATTTGAGCAGGTTGGAGTTGCTAATAACGCTGGCTATGATGAGATTCATGATGTTCATAGCTTAGTATCACCCAGAGGATTGTCACGGGATTCATTGAATAAGCTACCGCACCATATGGTTTCGAAGGGCATGAAGGCAGAGGATACTTTCTGTGCAATATGTTTGCAGGACATTGAAGTTGGAGAAGTTGCAAGAAGCTTACCTCGTTGCGACCATACTTTTCACTTGATATGCGTGGACAAATGGCTTGTCAAGAATGATTCGTGTCCTATTTGCAGACAGAATGTGTAG
Protein sequence

>Msa1212250

METGGRNEIPFLGFRFVSKFLLCAFSGTLTACFAFAGALTGAIAGAFAAKATKSGFLRGVSLGAIAGAILSVEVLEASRAYWCMEQTGSRGASSMADFIEELVRGRLVEESLTPAILTAYNLQFEQVGVANNAGYDEIHDVHSLVSPRGLSRDSLNKLPHHMVSKGMKAEDTFCAICLQDIEVGEVARSLPRCDHTFHLICVDKWLVKNDSCPICRQNV*